CN109769805A - A kind of emulsifiable pulvis of beauveria bassiana and preparation method thereof - Google Patents
A kind of emulsifiable pulvis of beauveria bassiana and preparation method thereof Download PDFInfo
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- CN109769805A CN109769805A CN201910244121.3A CN201910244121A CN109769805A CN 109769805 A CN109769805 A CN 109769805A CN 201910244121 A CN201910244121 A CN 201910244121A CN 109769805 A CN109769805 A CN 109769805A
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- beauveria bassiana
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- pulvis
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Abstract
The present invention relates to pesticide processing fields, in particular to emulsifiable pulvis of a kind of beauveria bassiana and preparation method thereof.The weight percentage of the emulsifiable pulvis is as follows: beauveria bassiana conidia powder 5%~15%；Solvent 3%~7%；Wetting agent 3%~7%；Dispersing agent 3%~7%；Carrier surplus.Its is safe and environment-friendly, storage is stable, good drug efficacy.
Description
Technical field
The present invention relates to pesticide processing fields, in particular to a kind of emulsifiable pulvis of beauveria bassiana and its preparation
Method.
Background technique
Rice water weevil (Lissorhoptrus oryzophilus Kuschel) belongs to coleoptera, Curculionidae, national two classes inspection
Epidemic disease pest is the important plant quarantine object in China.It originates in southeastern US open country and mountain forest, belongs to Strategy of Alien Invasive Species,
It is distributed in China rice producing region, is the primary pest in Rice Production, seriously affects the yield of rice.Rice water weevil harm
Caused production loss is 10%~30% in the U.S., is to be only second to planthopper and rice two in Japan up to 41%~67%
Change the important pests of snout moth's larva.Beauveria bassiana (Beauveria bassiana) is that the important Insect Pathogenic of research and application at most is true
Bacterium, host range is wide, can parasitize more than 15 purposes 700 insect.Beauveria bassiana, which has, does not generate pollution to environment, to people
Poultry safety can recycle the advantages that infecting pest again, it has also become one of hot spot of biological control research.Beauveria bassiana is biology
The best entomogenous fungi of rice water weevil is prevented and treated, the muscardine product of different dosage forms is widely applied in rice main producing region.
The formulation of microbial bacterial agent is one of biological pesticide key application technology, is easy to use and improves drug effect,
Convenient for standardized production and management.But biological pesticide is especially or body preparation must obtain stable physical behavior, survives and anti-
Certain time will be had by controlling effect.
In view of this, the present invention is specifically proposed.
Summary of the invention
Inventor of the invention is dedicated to the research of the emulsifiable pulvis of beauveria bassiana, and its purpose is to provide a kind of peaces
Entirely, environmentally friendly, storage stabilization, good drug efficacy the emulsifiable pulvis of beauveria bassiana.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
The present invention relates to a kind of emulsifiable pulvis of beauveria bassiana, weight percentage is as follows:
Beauveria bassiana conidia powder 5%~15%；
Solvent 3%~7%；
Wetting agent 3%~7%；
Dispersing agent 3%~7%；
Carrier surplus.
According to an aspect of the present invention, the invention further relates to the preparation sides of the emulsifiable pulvis of beauveria bassiana as described above
Method, comprising:
Each material component is mixed.
According to an aspect of the present invention, the invention further relates to the emulsifiable pulvis of beauveria bassiana as described above to prevent and treat
Application on cereal crops in pest.
Compared with prior art, the invention has the benefit that
(1) a small amount of organic solvent is only used in formula, and less toxic using solvent, both saved a large amount of organic solvent, avoided
Harm of the organic solvent in production and use to people and environment, and the safety of production, storage, transport can be improved；
(2) the emulsifiable pulvis bioactivity prepared is high, and safety is good, good drug efficacy；
(3) preparation method is simple, good product mobility, does not bond, does not agglomerate；
(4) product storge quality is good.
Specific embodiment
The present invention relates to a kind of emulsifiable pulvis of beauveria bassiana, weight percentage is as follows:
Beauveria bassiana conidia powder 5%~15%；
Solvent 3%~7%；
Wetting agent 3%~7%；
Dispersing agent 3%~7%；
Carrier surplus.
Preferably, the emulsifiable pulvis of beauveria bassiana as described above, weight percentage are as follows:
Beauveria bassiana conidia powder 7%~13%；
Solvent 4%~6%；
Wetting agent 4%~6%；
Dispersing agent 4%~6%；
Carrier surplus.
It is furthermore preferred that the emulsifiable pulvis of beauveria bassiana as described above, weight percentage are as follows:
Beauveria bassiana conidia powder 10%；
Solvent 5%；
Wetting agent 5%；
Dispersing agent 5%；
Carrier surplus.
Preferably, the emulsifiable pulvis of beauveria bassiana as described above, the solvent be selected from Span, Triton X-100,
One of tween, vegetable oil are a variety of；
It is furthermore preferred that the Span in Span 20, Span 40, Span 60, Span 80 or Span 100 at least one
Kind；
It is furthermore preferred that the tween is selected from least one of polysorbas20, polysorbate40, polysorbate60, polysorbate65 or Tween 80；
Preferably, the vegetable oil be selected from Semen setariae oil, peanut oil, soybean oil, olive oil, rilanit special, Canola oil,
At least one of rice bran oil, grape seed oil, sunflower oil, capric acid and palmitinic acid.
It is furthermore preferred that weight percentage, the solvent is selected from 1%~2% Tween 80 and 2%~5% plant
Oil.
Preferably, the emulsifiable pulvis of beauveria bassiana as described above, the wetting agent are selected from methyl glycol fatty acid ester, alkane
At least one of base phenol polyethenoxy ether, sucrose fatty ester, fatty acid esters of sorbitan and polyglyceryl fatty acid ester.
Preferably, the emulsifiable pulvis of beauveria bassiana as described above, the dispersing agent are selected from the south Wei Laihui KDS95, henry
This steps at least one of T-1004, NP-10, sodium butylnaphthalenesulfonate salt, polycarboxylate, Dispersant MF.
Preferably, the emulsifiable pulvis of beauveria bassiana as described above, the carrier are selected from diatomite, kaolin, bumps
At least one of stick soil, bentonite, precipitated calcium carbonate, clay and White Carbon black.
Preferably, the emulsifiable pulvis of beauveria bassiana as described above, the spore count in the beauveria bassiana conidia powder
Amount >=107cfu/g；More preferably 107~109cfu/g。
According to an aspect of the present invention, the invention further relates to the preparation sides of the emulsifiable pulvis of beauveria bassiana as described above
Method, comprising:
Each material component is mixed.
Preferably, method as described above adds remaining raw material after first mixing beauveria bassiana conidia powder and solvent
It mixes.
According to an aspect of the present invention, the invention further relates to the emulsifiable pulvis of beauveria bassiana as described above in prevention and treatment grain
Application on food crop in pest.
Preferably, as described above to apply, pest includes rice water weevil, rice-stem borer, corn on the cereal crops
Snout moth's larva, eating-core bean worm, sucking pest aleyrodid, aphid, grub, locust, colorado potato bug.
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
It can be with conventional products that are commercially available.
Specific embodiment
Examples 1 to 7, as the following table 1 takes the component of various contents to be prepared.The mode of preparation can be with are as follows:
Beauveria bassiana conidia powder, solvent are first stirred 30s~60s in high-speed mixer mixes well it, then plus
Enter wetting agent, dispersing agent and carrier, stirring 30s~60s to obtain the final product.
Table 1 prepares component and its content (weight g) used in the emulsifiable pulvis of beauveria bassiana
Comparative example
Using publication number CN101564047A, publication date is made in the embodiment 4 for the patent of invention on October 28th, 2009
The standby emulsifiable pulvis of obtained beauveria bassiana.
The measurement result of 2 product all technical of table
The preparation of emulsifiable pulvis is different from missible oil and wettable powder, it is by effective component or dissolution or to be diluted in suitable
Suitable has in organic solvent, then is adsorbed on water-soluble polymer powder or other kinds of water-soluble or water-insoluble powder carrier
It is formulated by processing, it is desirable that in the research of the dosage form, should consider that effective component is dissolved in (solubility in which kind of solvent
It is big), selected auxiliary agent can guarantee the stability of dilution, consider which kind of carrier is the effective component of dissolution be dispersed in again
On.The present invention is by optimizing each ingredient, so that the emulsifiable pulvis dispersion stabilization of the beauveria bassiana being prepared is good, heat storage is not
Easily decompose.
The emulsifiable pulvis of 2 beauveria bassiana of experimental example verifies the preventive effect of rice water weevil
1 test material
Strains tested: Strain of Beauveria bassiana
For trying plant: rice cultivar Ji round-grained rice 88
For trying insect: rice water weevil
2 test methods
The emulsifiable pulvis of 2.1 beauveria bassianas is prepared: being prepared by 4 method of embodiment
2.2 rice water weevils a situation arises investigation
Rice water weevil Incidence investigation is carried out to experimental plot before the application, 3 points are randomly selected in cell, each point is adjusted
100 cave rice are looked into, rice water weevil quantity are recorded, as insect population.
2.3 chemical applied to plants
The emulsifiable pulvis of prepared beauveria bassiana is configured to medical fluid by 150g/ mus of+3L water, it is equal with power spraye
It is even to spray in the cell, and set up blank control cell.
2.4 investigation
3d, 7d, 15d after administration randomly select 3 points for investigation, each points for investigation in processing cell and check plot
100 cave rice are investigated, rice water weevil quantity is recorded.Using the progress data preparation analysis of Microsoft Excel software, and according to
The formula death rate=dead borer population × 100/ insect population, corrected mortality=(the processing group death rate-control group death rate) ×
100/ (the 100- control group death rate) calculates corrected mortality.
3 results and analysis
Beauveria bassiana prevents and treats rice water weevil test result:
Rice water weevil quantity after table 3 is administered
4 control efficiency of table
| The processing group death rate (%) | The control group death rate (%) | Corrected mortality (%) | |
| 3d | 24.14 | 7.14 | 18.30 |
| 7d | 58.62 | 21.43 | 47.34 |
| 15d | 72.41 | 17.86 | 66.42 |
From in table 3 and table 4 as can be seen that the emulsifiable pulvis of beauveria bassiana prevention and treatment rice water weevil on have preferable prevention and treatment
Effect, 3 days corrected mortalities reach 18.30% after administration, close to 20%；7d corrected mortality has reached after administration
47.34%；The application 15d post-equalization death rate has been up to 66.42%, can be guaranteed with the harm of effectively preventing rice water weevil
Rice yield.
The emulsifiable pulvis of 3 beauveria bassiana of experimental example verifies the preventive effect of corn borer
1 test material
Reagent agent: the emulsifiable pulvis of muscardine prepared by embodiment 1.
For trying plant: corn planting kind lucky single 558
For trying insect: corn borer
2) test method:
Test sets 2 processing, respectively medicament group and blank control group, 3 repetitions of each processing.Medicament group presses 200g/
Mu+15L aqua amount is configured to medical fluid, uniformly sprays in the cell.Control group does not use any control measure.
3) investigation method
Using investigation after autumn, each 5 points of random searching of repetition, 100 plants of plants of every investigation, respondent is worm living
Number, channel number, killed strain number.
4) test result:
Revision insect recluced rate, worm channel decline rate, killed strain decline rate and control efficiency are calculated according to following formula:
Revision insect recluced rate is calculated by formula:
Revision insect recluced rate=(check plot insect population rate-treatment region insect population rate) × 100%/check plot insect population rate
Worm channel decline rate is calculated by formula:
Worm channel decline rate=(check plot worm channel rate-treatment region worm channel rate) × 100%/check plot worm channel rate
Killed strain decline rate is calculated by formula:
Killed strain decline rate=(check plot attacked trees rate-treatment region attacked trees rate) × 100%/check plot attacked trees rate
Average control efficiency is calculated by formula:
Average control efficiency=(Revision insect recluced rate+worm channel decline rate+killed strain decline rate)/3
The test result of each processing prevention and treatment corn borer is shown in Table 5.
Table 5 respectively handles the test result of prevention and treatment corn borer
As can be seen from Table 5, medicament group is significantly lower than control group at 3 borer population living, channel number, killed strain number aspects,
Face is above beauveria bassiana granular formulation and significant difference, and it is fine that this just illustrates that the emulsifiable pulvis of muscardine has corn field corn borer
Control efficiency.
The emulsifiable pulvis of 4 beauveria bassiana of experimental example verifies the preventive effect of rice-stem borer
The emulsifiable pulvis of 4 muscardine of embodiment is used for the prevention and treatment of rice-stem borer, observes the prevention and treatment to rice-stem borer
Effect.
2) test material:
Reagent agent: the emulsifiable pulvis of muscardine prepared by embodiment 4.
For trying plant: rice cultivar Ji round-grained rice 88
For trying insect: rice-stem borer
3) test method:
2 processing of test setting, respectively medicament group and control group, 3 repetitions of each processing.Medicament group by 200g/ mus+
15L aqua amount is configured to medical fluid, is administered in rice tillering peak period and heading stage, uniformly sprays in cell respectively, control group is not
With any control measure.
4) investigation method:
Randomly select in cell at 5 points, every 100 cave rice of investigation.Withered sheath number, withered calculation are investigated at heading stage；Pregnant
Ear period investigates dead ears number.
5) test result:
Withered heart rate preventive effect, withered sheath rate preventive effect, the calculation formula of dead ears rate preventive effect and average preventive effect are as follows:
Withered heart rate preventive effect is calculated by formula:
Withered heart rate preventive effect=(check plot withered heart rate-treatment region withered heart rate) × 100%/check plot withered heart rate
Withered sheath rate preventive effect is calculated by formula:
Withered sheath rate preventive effect=(the withered withered sheath rate of sheath rate-treatment region in check plot) withered sheath rate in × 100%/check plot
Dead ears rate preventive effect is calculated by formula:
Dead ears rate preventive effect=(check plot dead ears rate-treatment region dead ears rate) × 100%/check plot dead ears rate
Average control efficiency is calculated by formula:
Average control efficiency=(dead ears rate preventive effect+withered heart rate preventive effect+withered sheath rate preventive effect)/3
The test result of each processing prevention and treatment rice-stem borer is shown in Table 6.
Table 6 respectively handles the test result of prevention and treatment rice-stem borer
As can be seen from Table 5, medicament group is significantly lower than rice-stem borer in terms of withered heart rate, withered sheath rate, dead ears rate
Control group illustrates that the emulsifiable pulvis of muscardine has preferable control efficiency to rice-stem borer.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, but those skilled in the art should understand that: its
It is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features
It is equivalently replaced；And these are modified or replaceed, various embodiments of the present invention skill that it does not separate the essence of the corresponding technical solution
The range of art scheme.
Claims (10)
1. a kind of emulsifiable pulvis of beauveria bassiana, which is characterized in that its weight percentage is as follows:
Beauveria bassiana conidia powder 5%~15%；
Solvent 3%~7%；
Wetting agent 3%~7%；
Dispersing agent 3%~7%；
Carrier surplus.
2. the emulsifiable pulvis of beauveria bassiana according to claim 1, which is characterized in that its weight percentage is as follows:
Beauveria bassiana conidia powder 7%~13%；
Solvent 4%~6%；
Wetting agent 4%~6%；
Dispersing agent 4%~6%；
Carrier surplus.
3. the emulsifiable pulvis of beauveria bassiana according to claim 1 or 2, which is characterized in that the solvent be selected from Span,
One of Triton X-100, tween, vegetable oil are a variety of；
Preferably, the Span is selected from least one of Span 20, Span 40, Span 60, Span 80 or Span 100；
Preferably, the tween is selected from least one of polysorbas20, polysorbate40, polysorbate60, polysorbate65 or Tween 80；
Preferably, the vegetable oil is selected from Semen setariae oil, peanut oil, soybean oil, olive oil, rilanit special, Canola oil, rice bran
At least one of oil, grape seed oil, sunflower oil, capric acid and palmitinic acid.
4. the emulsifiable pulvis of beauveria bassiana according to claim 1 or 2, which is characterized in that the wetting agent is selected from third
Diol fatty acid ester, alkyl phenol polyoxyethylene ether, sucrose fatty ester, fatty acid esters of sorbitan and polyglycerol fatty acid
At least one of ester.
5. the emulsifiable pulvis of beauveria bassiana according to claim 1 or 2, which is characterized in that the dispersing agent is selected from prestige
Carry out favour south KDS95, Hensel steps at least one of T-1004, NP-10, sodium butylnaphthalenesulfonate salt, polycarboxylate, Dispersant MF.
6. the emulsifiable pulvis of beauveria bassiana according to claim 1 or 2, which is characterized in that the carrier is selected from diatom
At least one of soil, kaolin, attapulgite, bentonite, precipitated calcium carbonate, clay and White Carbon black.
7. the emulsifiable pulvis of beauveria bassiana according to claim 1 or 2, which is characterized in that the beauveria bassiana spore
Spore quantity >=10 in sub- powder7cfu/g。
8. the preparation method of the emulsifiable pulvis of the described in any item beauveria bassianas of claim 1~7 characterized by comprising
Each material component is mixed.
9. according to the method described in claim 8, it is characterized in that, after first beauveria bassiana conidia powder and solvent are mixed, then
The mixing of remaining raw material is added.
10. the emulsifiable pulvis of the described in any item beauveria bassianas of claim 1~7 answering in pest on prevention and treatment cereal crops
With；
Preferably, pest includes rice water weevil, rice-stem borer, corn borer, eating-core bean worm, pierce-suck type on the cereal crops
Pest aleyrodid, aphid, grub, locust, colorado potato bug.
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| CN201910244121.3A CN109769805A (en) | 2019-03-28 | 2019-03-28 | A kind of emulsifiable pulvis of beauveria bassiana and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415210A (en) * | 2002-12-16 | 2003-05-07 | 张泽华 | Wettability powder of fungi spore |
| WO2009008260A1 (en) * | 2007-07-10 | 2009-01-15 | Nichigo-Mowinyl Co., Ltd. | Aqueous synthetic-resin emulsion for cement mortar admixture, re-emulsifiable powder for cement mortar admixture from aqueous synthetic-resin emulsion, and cement mortar admixture comprising the same |
| CN101564047A (en) * | 2009-05-27 | 2009-10-28 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN104770397A (en) * | 2015-01-24 | 2015-07-15 | 吉林省农业科学院 | Beauveria bassiana pulvis and application thereof |
| CN106879636A (en) * | 2017-04-17 | 2017-06-23 | 吉林省农业科学院 | Muscardine wettable powder and its application |
-
2019
- 2019-03-28 CN CN201910244121.3A patent/CN109769805A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415210A (en) * | 2002-12-16 | 2003-05-07 | 张泽华 | Wettability powder of fungi spore |
| WO2009008260A1 (en) * | 2007-07-10 | 2009-01-15 | Nichigo-Mowinyl Co., Ltd. | Aqueous synthetic-resin emulsion for cement mortar admixture, re-emulsifiable powder for cement mortar admixture from aqueous synthetic-resin emulsion, and cement mortar admixture comprising the same |
| CN101564047A (en) * | 2009-05-27 | 2009-10-28 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN104770397A (en) * | 2015-01-24 | 2015-07-15 | 吉林省农业科学院 | Beauveria bassiana pulvis and application thereof |
| CN106879636A (en) * | 2017-04-17 | 2017-06-23 | 吉林省农业科学院 | Muscardine wettable powder and its application |
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