CN106359454A - Preparation method of empedobacter brevis pesticide - Google Patents
Preparation method of empedobacter brevis pesticide Download PDFInfo
- Publication number
- CN106359454A CN106359454A CN201610790708.0A CN201610790708A CN106359454A CN 106359454 A CN106359454 A CN 106359454A CN 201610790708 A CN201610790708 A CN 201610790708A CN 106359454 A CN106359454 A CN 106359454A
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- Prior art keywords
- empedobacter brevis
- pesticide
- empedobacter
- brevis
- gxw15
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Abstract
The invention relates to a preparation method of empedobacter brevis pesticide and belongs to the technical field of microorganisms. After the prepared empedobacter brevis pesticide is sprayed to plant epidermis, empedobacter brevis GXW 15-4 is sucked into plant injurious insects through mouths, plant lepidoptera pests die due to lesion within 24 h. The empedobacter brevis pesticide is safe to use, does not pollute the environment and has no hazardous effect on plants, natural enemies of pests, mammals and aquatic animals.
Description
The application is the divisional application of Empedobacter brevis pesticide and its manufacture method, the applying date of original application: August 9 in 2011
Day, application number: 201110234995.4, title: Empedobacter brevis pesticide and its manufacture method.
Technical field
The present invention relates to a kind of preparation method of Empedobacter brevis pesticide, belong to microbial technology field.
Background technology
The primary pest of various plants is lepidoptera pest, for example Pieris rapae, diamondback moth, Prodenia litura, water cnaphalocrocis
Worm, pink rice borer, striped rice borer, yellow rice borer, cotton sandfly worm, fruit tree Limacodidae.It is the major measure of preventing and treating plant insect to plant applying pesticides.
With the progress of social development and the mankind, people increasingly worry to agricultural product chemical contamination, urgently expect with biological pesticide
Substitute chemical pesticide.For this reason, a lot of developed countries put into sizable manpower, financial resources development biological pesticide in the world, but biological
Antibacterial product development more, biopesticide products development progress is unhappy.The just pickles stronger to chemical pesticide Drug resistance
For the biological pesticide development and application of the great lepidoptera pest such as moth, Prodenia litura, rice leaf folder, main on domestic and international market
Take product how remain German Bel (berliner) found before 100 years and study manufacture successful Bacillus thuringiensis parasite killing
Agent (bt), not yet has other more preferable substitute products so far, and more not useful Empedobacter brevis gxw15-4 manufacture insecticide
Precedent.It is noted that in recent years, domestic and international pesticide market occurs in that some plant insecticide kinds, but because of plant
Insecticide is subject to plant variety and resource limit, and industrialization production difficulty is larger, and because plant insecticide development cost is universal
Higher, peasant household selects the enthusiasm using plant insecticide not high.
Content of the invention
The present invention be directed to plant Severe pests produce certain Drug resistance and people to using chemical agriculture to chemical pesticide
The present situation that medicine is generally worried, proposes a kind of preparation method of Empedobacter brevis pesticide.Using the pesticide obtained by biofermentation technique
Using safety, environmentally safe, make to plant itself, to pest natural enemy, to mammal and aquatic animal all nonhazardouss
With.
The present invention solves a kind of technical problem: preparation method of Empedobacter brevis pesticide by the following technical programs, including with
Lower step:
Step one, dissect fall ill death insect corpse, separate, extract Empedobacter brevis gxw15-4 bacterial strain；
Step 2, by separating, the bacterial strain that extracts test tube lb slant culture 24～36 hours, 24～30 DEG C of temperature, light
According to 3000～5000lx, ph value 7～7.2；
Step 3, the Prodenia litura that Empedobacter brevis gxw15-4 turned out are inoculated on field crop high instar larvae body
Inside carry out field domestication, then screening Empedobacter brevis gxw15-4 are excellent from death rate fast Prodenia litura advanced age larva corpse
Bacterial strain, is inoculated in spawn culture bottle, carries out one grade fermemtation with shaking table culture method, fermentation condition is identical with step 2；
Step 4, the three grade fermemtation device being 10 times with volume gradient fermentation, plus suitable quantity of water and Empedobacter brevis gxw15-4 are former
Kind, stir into mixed liquor, condition of culture is identical with step 2, form Empedobacter brevis suspension, hang through bottled formation Empedobacter brevis
Floating agent product.
The solution that described Empedobacter brevis suspension is directly diluted to 1-1.3 hundred million spores/ml content is used for plant Lepidoptera
The preventing and treating of insect.Described suspension is to hang containing the milky more than or equal to 10,000,000,000 spores/ml Empedobacter brevis gxw15-4 live body
Supernatant liquid.
In the described step 4 of above method, the culture matrix of fermentation is using the Semen sojae atricolor powder after sterilizing or soy milk powder
4.5%th, dried silkworm chrysalis meal 3%, calcium powder 1%, Sal 0.5%.
The present invention further provides the preparation method of Empedobacter brevis mother's medicine, based on the above method, also include following
One grade fermemtation product in Empedobacter brevis suspension manufacture process or second order fermentation product, are placed at a high speed at step 5 by step
Carry out premature dehydration process in centrifuge；Step 6, the product three-level freezer dryer after high speed centrifugation processed is entered
Row dried, temperature controls in -5 DEG C, -15 DEG C, -75 DEG C respectively so as to become the powder that water content is less than 5%, packs shape
Become Empedobacter brevis mother's medicine.
The solution that described Empedobacter brevis mother's medicine is directly diluted to 1-1.3 hundred million spores/ml content is used for plant insect to be prevented and treated,
Also other dosage forms of Empedobacter brevis pesticide can be formed through deep processing, also can control other disease pest of plant for simultaneous after compounding with chemical pesticide
Evil.Described Empedobacter brevis mother's medicine is the pale yellow powder having more than or equal to 30,000,000,000 spores/mg Empedobacter brevis gxw15-4 live body.
Empedobacter brevis (empedobacter brevis) gxw15-4 is Zhenjiang Runyu Biological Science and Technology Development Co., Ltd.
A kind of plant insect cause of disease that senior agronomist Gao little Wen finds in the Prodenia litura corpse that calendar year 2001 September dissects death of falling ill
Bacterium.This bacterium is deposited in China typical culture collection center (abbreviation cctcc), deposit number cctcc on December 11st, 2002
No:m202046, preservation address Wuhan, China Wuhan University.
Empedobacter brevis gxw15-4 individual morphology is characterized as: thalline is in rod-short, long 2.6-3.8 μm, wide 0.8-1.1 μm, two
End blunt circle, has about 1 μm with scanning electron microscopic observation inside it3Quadratic crystal shape.
According to Institute of Microorganism, Academia Sinica's Testing and appraisal report ([2009] micro- searching the 175th), Empedobacter brevis
Gxw15-4 Physicochemical test the results are shown in Table 1:
Table 1 Empedobacter brevis pesticide Physicochemical test result (biolig gn2) table
Empedobacter brevis gxw15-4 regeneration capacity is strong, can under the conditions of batch production amount reproduction, its propagation substrate mainly adopts
Semen sojae atricolor powder after sterilizing, soy milk powder, dried silkworm chrysalis meal etc., main matrix raw material is that on Renewable resource, and domestic and international market, the source of goods is rich
Richness, completes to meet the needs of industrialization production.
After plant epidermis, the oral inspiration of Empedobacter brevis gxw15-4 enters to plant the Empedobacter brevis pesticide application that the present invention is obtained
In thing insect body, in 24 hours, lead to plant lepidoptera pest pathological changes dead.Using safety, environmentally safe, to plant basis
Body, to pest natural enemy, to mammal and aquatic animal nonhazardouss effect.
The present invention selects microorganism fermentation process to manufacture Empedobacter brevis suspending agent, select fermentable, dewatering process system
Make Empedobacter brevis mother's medicine, using safety, environmentally safe, to plant itself, to pest natural enemy, to mammal and aquatic
Animal all nonhazardouss effects.
Brief description
Fig. 1 is the schematic flow sheet of the inventive method.
Specific embodiment
Embodiment
The present embodiment contains suspending agent and the preparation method of female medicine, as shown in figure 1, specific as follows:
1st, Empedobacter brevis pesticide manufacture method
Empedobacter brevis gxw15-4 can adopt microorganism three grade fermemtation technology amount reproduction.
Empedobacter brevis gxw15-4 tunning can directly be packaged into Empedobacter brevis suspending agent, and its manufacturing step is:
(1) dissect the plant insect corpse of death of falling ill, separate, extract Empedobacter brevis gxw15-4 bacterial strain.
(2) by separating, the bacterial strain that extracts test tube lb slant culture 24～36 hours, 24～30 DEG C of temperature, illumination
3000～5000lx, ph value 7～7.2.
(3) select the energetic strain excellent in test tube lb inclined-plane, with shaking table culture method amplification culture, condition of culture and step
(2) identical.
(4) the three grade fermemtation device being 10 times with volume gradient ferments, using the Semen sojae atricolor powder (or soy milk powder) after sterilizing
4.5%th, dried silkworm chrysalis meal 3%, calcium powder 1%, Sal 0.5% etc. is as culture matrix, plus suitable quantity of water and Empedobacter brevis gxw15-4 former
Kind, stir into mixed liquor, condition of culture is identical with step (2), form Empedobacter brevis suspension, hang through the bottled Empedobacter brevis that are
Floating agent product.
Empedobacter brevis mother's medicine is through high speed centrifugation with Empedobacter brevis gxw15-4 one grade fermemtation product or second order fermentation product
The powdered product that dehydration, three-level lyophilization are formed, its manufacturing step is:
(1) by the one grade fermemtation product in Empedobacter brevis suspension manufacture process or second order fermentation product place at a high speed from
Carry out premature dehydration process in scheming；
(2) the product three-level freezer dryer after high speed centrifugation processed is dried process, temperature is controlled respectively
In -5 DEG C, -15 DEG C, -75 DEG C so as to become the powder that water content is less than 5%, after comminution by gas stream, packaging forms short steady bar to system
Starter medicine.
The aqueous solution that Empedobacter brevis suspending agent can directly be diluted to 1-1.3 hundred million spores/ml content uniformly sprays in axis
The positive and negative epidermis with blade, after the oral inspiration of lepidoptera pest enters in vivo, in 24 hours, just pathological changes are dead.
Empedobacter brevis mother's medicine can directly be diluted to 1-1.3 hundred million spores/ml content aqueous solution uniformly spray in axis and
The positive and negative epidermis of blade is used for the preventing and treating to plant lepidoptera pest.
Empedobacter brevis mother's medicine also deep processing can form the Empedobacter brevis pesticide of other dosage forms for preventing to lepidoptera pest
Control, also can control other pest and disease damages of plant for simultaneous after compounding with chemical pesticide.
2nd, Empedobacter brevis agricultural insecticide effect
Measure through Agricultural University Of Nanjing, Empedobacter brevis suspending agent has obvious insecticidal activity to diamondback moth larvae.Through environment
Protection portion Nanjing Institute of Environmental Science tests, and Empedobacter brevis suspending agent field is normal to be used to Brachydanio rerio, large-scale, bead
Algae, Apiss, Carnis Coturnicis japonicae are low-risk.Through Jiangsu Prov. Disease Preventing and Controlling Center's inspection, Empedobacter brevis suspending agent is to male and female rat
Belong to micro- poison level, to rabbit no skin irritation, belong to weak sensitizer.Through CDC occupational health and poisoning control
System is checked, and Empedobacter brevis suspending agent is to human body no pathogenicity.
China Agricultural University's agronomy and Biotechnology Institute, Institute of Plant Protection of academy of agricultural sciences of Henan Province, Institute of Plant Protection of academy of agricultural sciences of Shandong Province,
The test report summarized results that four authoritative institutions such as Jiangsu Institute for Pesticides provide show: Empedobacter brevis suspending agent is after medicine
In 7 days, the fatality rate of diamondback moth, Spodoptera litura larvae is improved day by day.The 7th day after medicine, 10,000,000,000 spores/ml Empedobacter brevis suspend
800 times of liquid of agent, 900 times of liquid are higher than all that 16000iu/mg Bacillus thuringiensis are wettable to the fatality rate of diamondback moth, Spodoptera litura larvae
Property 1000 times of liquid of powder；10000000000 spores/1000 times of liquid of ml Empedobacter brevis suspending agent to diamondback moth, Spodoptera litura larvae lethal
Rate is slightly below 1000 times of liquid of 16000iu/mg Bacillus thuringiensis wettable powder.
According to another plant protection master station of Anhui Province, plant protection station of Jiangsu Province respectively at 2010 to vegetable-crop pest-insect insecticidal effect
Demonstration test report collects, and 10,000,000,000 spores/600 times of liquid of ml Empedobacter brevis suspending agent, 800 times of liquid, 1000 times of liquid are right in medicine 7 days
The median lethal rate of diamondback moth larvae is respectively 81.78%, 77.17%, 70.88%, is all better than 16000iu/mg Su Yun gold bar
500 times of liquid (being shown in Table 2) of bacterium wettable powder；10000000000 spores/600 times of liquid of ml Empedobacter brevis suspending agent, 800 times of liquid are right in medicine 7 days
The median lethal rate of Spodoptera litura larvae is respectively 85.11%, 83.30%, is above 16000iu/mg Bacillus thuringiensis wettable
Property 500 times of liquid of powder；10000000000 spores/1000 times of liquid drug effects of ml Empedobacter brevis suspending agent and 16000iu/mg Bacillus thuringiensis can
500 times of liquid phases of WP imitate (being shown in Table 3).
Table 2 two places demonstration test is to diamondback moth insecticidal effect summary sheet (%)
Table 3 two places demonstration test is to Prodenia litura insecticidal effect summary sheet (%)
In addition to above-mentioned enforcement, the present invention can also have other embodiment.All formed using equivalent or equivalent transformation
Technical scheme, all fall within the protection domain of application claims.
Claims (7)
1. a kind of preparation method of Empedobacter brevis pesticide, comprises the following steps:
Step one, dissect fall ill death insect corpse, separate, extract Empedobacter brevis gxw15-4 bacterial strain；
Step 2, by separating, the bacterial strain that extracts test tube lb slant culture 24～36 hours, 24～30 DEG C of temperature, illumination
3000～5000lx, ph value 7～7.2；
Enter in step 3, the Prodenia litura that Empedobacter brevis gxw15-4 turned out are inoculated on field crop high instar larvae body
Row field is tamed, then screens Empedobacter brevis gxw15-4 strain excellent from the fast Prodenia litura advanced age larva corpse of death rate,
It is inoculated in spawn culture bottle, carries out one grade fermemtation with shaking table culture method, fermentation condition is identical with step 2；
Step 4, the three grade fermemtation device being 10 times with volume gradient fermentation, plus suitable quantity of water and Empedobacter brevis gxw15-4 original seed,
Stir into mixed liquor, condition of culture is identical with step 2, form Empedobacter brevis suspension, through bottled formation Empedobacter brevis suspending agent
Product.
2. according to claim 1 Empedobacter brevis pesticide preparation method it is characterised in that: in described step 4 fermentation training
Foster substrate is using the Semen sojae atricolor powder after sterilizing or soy milk powder 4.5%, dried silkworm chrysalis meal 3%, calcium powder 1%, Sal 0.5%.
3. according to claim 1 Empedobacter brevis pesticide preparation method it is characterised in that: further comprising the steps of, step
5th, the one grade fermemtation product in Empedobacter brevis suspension manufacture process or second order fermentation product are placed in high speed centrifuge
Row premature dehydration is processed；Step 6, the product three-level freezer dryer after high speed centrifugation processed is dried process,
Temperature controls in -5 DEG C, -15 DEG C, -75 DEG C respectively so as to become the powder that water content is less than 5%, it is female that packaging forms Empedobacter brevis
Medicine.
4. according to claim 1 Empedobacter brevis pesticide preparation method it is characterised in that: described Empedobacter brevis suspension is straight
Connect the preventing and treating being diluted to the solution of 1-1.3 hundred million spores/ml content for plant lepidoptera pest.
5. according to claim 4 Empedobacter brevis pesticide preparation method it is characterised in that: described suspension is containing being more than
Milk-white coloured suspension equal to 10,000,000,000 spores/ml Empedobacter brevis gxw15-4 live body.
6. according to claim 1 Empedobacter brevis pesticide preparation method it is characterised in that: described Empedobacter brevis mother's medicine is direct
Be diluted to 1-1.3 hundred million spores/ml content solution be used for plant insect prevent and treat, also can through deep processing formed Empedobacter brevis pesticide its
His dosage form, also can control other pest and disease damages of plant for simultaneous after compounding with chemical pesticide.
7. according to claim 6 Empedobacter brevis pesticide preparation method it is characterised in that: described Empedobacter brevis mother's medicine is tool
There is the pale yellow powder more than or equal to 30,000,000,000 spores/mg Empedobacter brevis gxw15-4 live body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610790708.0A CN106359454A (en) | 2011-08-09 | 2011-08-09 | Preparation method of empedobacter brevis pesticide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102349954A CN102342299A (en) | 2011-08-09 | 2011-08-09 | Empedobacter brevis pesticide and preparation method thereof |
| CN201610790708.0A CN106359454A (en) | 2011-08-09 | 2011-08-09 | Preparation method of empedobacter brevis pesticide |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011102349954A Division CN102342299A (en) | 2011-08-09 | 2011-08-09 | Empedobacter brevis pesticide and preparation method thereof |
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| CN106359454A true CN106359454A (en) | 2017-02-01 |
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| CN201610790708.0A Pending CN106359454A (en) | 2011-08-09 | 2011-08-09 | Preparation method of empedobacter brevis pesticide |
| CN2011102349954A Pending CN102342299A (en) | 2011-08-09 | 2011-08-09 | Empedobacter brevis pesticide and preparation method thereof |
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| WO (1) | WO2013020350A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353899A (en) * | 2018-02-09 | 2018-08-03 | 广东省林业科学研究院 | The compounded pesticides and its application of green muscardine fungus and Empedobacter brevis |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106359454A (en) * | 2011-08-09 | 2017-02-01 | 镇江市润宇生物科技开发有限公司 | Preparation method of empedobacter brevis pesticide |
| CN103333831A (en) * | 2013-07-01 | 2013-10-02 | 镇江市润宇生物科技开发有限公司 | Method for outdoor domestication and purification of empedobacter brevis GXW15-4 |
| CN103409344A (en) * | 2013-07-18 | 2013-11-27 | 镇江市润宇生物科技开发有限公司 | Soymilk matrix and method for fermenting Empedobacter brevis GXW15-4 through using it |
| CN105316250B (en) * | 2014-11-28 | 2018-08-03 | 浙江大学 | One plant of Empedobacter brevis and its application in preparing chiral alcohol |
| CN106489994B (en) * | 2016-08-31 | 2019-03-29 | 江苏天容集团股份有限公司 | Pesticidal combination containing Empedobacter brevis and dimehypo |
| CN106376598B (en) * | 2016-08-31 | 2019-02-12 | 江苏天容集团股份有限公司 | Pesticidal combination containing Empedobacter brevis and Cupric sulfate |
| CN111296472A (en) * | 2020-04-01 | 2020-06-19 | 京博农化科技有限公司 | Insecticidal composition containing indoxacarb and Empedobacter brevis and application thereof |
| CN116925943A (en) * | 2022-03-29 | 2023-10-24 | 扬州大学 | Pseudomonas and application thereof |
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| CN106359454A (en) * | 2011-08-09 | 2017-02-01 | 镇江市润宇生物科技开发有限公司 | Preparation method of empedobacter brevis pesticide |
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2011
- 2011-08-09 CN CN201610790708.0A patent/CN106359454A/en active Pending
- 2011-08-09 CN CN2011102349954A patent/CN102342299A/en active Pending
-
2012
- 2012-04-27 WO PCT/CN2012/000563 patent/WO2013020350A1/en active Application Filing
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| CN1397520A (en) * | 2002-07-26 | 2003-02-19 | 福建省尤溪县绿地生物制品有限公司 | Composite bacterial microbe fertilizer and its preparing process |
| CN1431296A (en) * | 2003-01-28 | 2003-07-23 | 高小文 | Insect pestilence germ and method of application for breeding destroy intestinal worms |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353899A (en) * | 2018-02-09 | 2018-08-03 | 广东省林业科学研究院 | The compounded pesticides and its application of green muscardine fungus and Empedobacter brevis |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013020350A1 (en) | 2013-02-14 |
| CN102342299A (en) | 2012-02-08 |
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Application publication date: 20170201 |