CN105410032B - A kind of microorganism trapping bait and application - Google Patents
A kind of microorganism trapping bait and application Download PDFInfo
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- CN105410032B CN105410032B CN201510958305.8A CN201510958305A CN105410032B CN 105410032 B CN105410032 B CN 105410032B CN 201510958305 A CN201510958305 A CN 201510958305A CN 105410032 B CN105410032 B CN 105410032B
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- 244000005700 microbiome Species 0.000 title claims abstract description 48
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 31
- 241000255588 Tephritidae Species 0.000 claims abstract description 27
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 13
- 235000009566 rice Nutrition 0.000 claims abstract description 13
- 241001517923 Douglasiidae Species 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 239000004480 active ingredient Substances 0.000 claims abstract description 4
- 230000002265 prevention Effects 0.000 claims description 13
- 240000007594 Oryza sativa Species 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 235000020971 citrus fruits Nutrition 0.000 abstract description 18
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 abstract description 10
- 241001124181 Bactrocera dorsalis Species 0.000 abstract description 7
- 239000003814 drug Substances 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 7
- 230000009471 action Effects 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 241000209094 Oryza Species 0.000 abstract 2
- ZYEMGPIYFIJGTP-UHFFFAOYSA-N O-methyleugenol Chemical compound COC1=CC=C(CC=C)C=C1OC ZYEMGPIYFIJGTP-UHFFFAOYSA-N 0.000 description 34
- 229940116837 methyleugenol Drugs 0.000 description 17
- PRHTXAOWJQTLBO-UHFFFAOYSA-N methyleugenol Natural products COC1=CC=C(C(C)=C)C=C1OC PRHTXAOWJQTLBO-UHFFFAOYSA-N 0.000 description 17
- 230000000694 effects Effects 0.000 description 14
- 239000000243 solution Substances 0.000 description 14
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- 239000005667 attractant Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 8
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- 241000607479 Yersinia pestis Species 0.000 description 7
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- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
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- 229920002472 Starch Polymers 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- YKPQYKBIAFGWBT-KAMYIIQDSA-N [(Z)-18-hydroxyoctadec-9-en-7-yl] acetate Chemical compound CCCCCCC(C\C=C/CCCCCCCCO)OC(C)=O YKPQYKBIAFGWBT-KAMYIIQDSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
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- 238000005516 engineering process Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
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- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
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- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
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- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
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- UMIKWXDGXDJQJK-UHFFFAOYSA-N Cuelure Chemical compound CC(=O)CCC1=CC=C(OC(C)=O)C=C1 UMIKWXDGXDJQJK-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000256626 Pterygota <winged insects> Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 241000975357 Salangichthys microdon Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000255632 Tabanus atratus Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
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- 244000042314 Vigna unguiculata Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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- 238000009395 breeding Methods 0.000 description 1
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- 239000001390 capsicum minimum Substances 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
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- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
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- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000003694 hair properties Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
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- 230000004899 motility Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
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- UJMBCXLDXJUMFB-UHFFFAOYSA-K trisodium;5-oxo-1-(4-sulfonatophenyl)-4-[(4-sulfonatophenyl)diazenyl]-4h-pyrazole-3-carboxylate Chemical group [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a kind of microorganism trapping bait and application.The active ingredient of the trapping bait is bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010 KC CGMCC No.5722 zymotic fluids, living bacteria count Han Liang≤3 × 109cfu/ml.The special odor that the trapping bait gives out can lure trypetid, drosophila and Americal rice leaf miner feeding, and male and female with catching, grow by the action period, cost is low, any chemical toxic medicament is not contained, does not kill and does not kill any other non-target organism, belong to environmentally friendly organism trapping agent.Trapping to dacus dorsalis：Than control increase by 18.81~23.71%, increase by 37.78%~30 times than compareing the female adult lured.Trapping to citrus fruit fly：Than control increase by 64.43%, traping female borer population increases by 33.25 times.Trapping to Americal rice leaf miner：Female adult rate accounts for 55~60%.
Description
Technical field
The invention belongs to agricultural sciences injurious insect control technical field, and in particular to a kind of microorganism trapping bait, and luring
Catch the application in male and female fruit fly, liriomyza bryoniae.
Background technology
Diptera (Diptera) is Arthropoda (Arthropoda), Mandibulata (Mandibulata), Insecta
(Insecta), 1 mesh of Pterigota (Pterygota), is that coleoptera, Lepidoptera, the 4th of Hymenoptera the are only second in Insecta
Big mesh.85000 kinds known to the world, distribution on global.More than 4000 plant known to China.Dipteral insect has a pair of of wing and a pair of of balance
Rod, including mosquito, fly, horsefly.Some monoids of common Diptera such as Hylemyia Platura Meigen, leaf are dived the larva of fly, fruit fly, wheat cecidomyiia etc., are all agricultures
The important pests of industry.Agromyzidae is caused harm a variety of legumes；The numerous species of Tephritidae are caused harm citrus, pears, peach etc..
Fruit fly belongs to Diptera Tephritidae (Tephritidae), is to cause heavy losses to fruits and vegetables class crop
A kind of important pests.Tephritidae hitherto known about 500 belongs to, and more than 4500 plant, wherein, fruits and vegetables class is harmful to the monoid of trypetid up to 15
Category, 22 subgenus, more than 150 are planted, and [Bactrocera dorsalis (Hendel)], melonfly are closed with dacus dorsalis in China
[Bactrocera cucurbitae (Coqil Lett)], citrus fruit fly [Bactrocera minax (Enderlein)] etc.
It is relatively conventional, it is distributed mainly on Hunan, Hubei, Sichuan, Chongqing, Guizhou, Fujian, Guangdong, Guangxi, Hainan, Yunnan, Taiwan etc.
Save.The host range of harmful trypetid is very wide, is related to economic fruit class plant and amounts to 24 sections, 42 categories：4 section of vegetable plants, 16
Belong to (wild host plant is not included).Fruit fly reproductive capacity is strong, harmfulness is big.In trypetid distribution or Endemic Area, post
Main fruit percentage of injury average out to 25-45%, up to 80% when serious, or even total crop failure, bring great economy to fruits and vegetables class crop
Loss, has seriously affected peasant and has increased production extra earning.
At present, the method for preventing Fruit fly mainly has cultural control, chemical prevention, physical control and biological control
Deng.Wherein cultural control turns over the pupa that goes out etc. including periodically removing the real band wormed fruit in plantation, fruit bagging and winter；Chemistry is anti-
Control measure and mainly spray Bait on Killing trypetid；Physical control measure uses the technology that trypetid trapping bait and poison bait are combined, mesh
Before, the trapping bait for preventing trypetid is mainly chemical synthesis trapping bait, such as lures ketone (cue lure), methyl eugenol
(methyleugenol) etc..Since cultural control measure is time consuming, effect is bad；Chemical prevention not only easily produces trypetid
The problem of giving birth to the resistance to the action of a drug, and people, animal be harmful to, are also easy to produce the environmental pollutions such as pesticide residue；Property trapping bait in practical applications
There is the problems such as volatilization is fast, the lasting period is short, medication is frequent again, do not reach the prevention purpose of control borer fruit rate, and apply at present
Most extensive trapping bait methyl eugenol only lures male worm etc., its application is restricted.
There are sugar-vinegar liquid, female hormone, protein three major types lure for trypetid both at home and abroad.Sugar-vinegar liquid is time-consuming, laborious, cost
It is higher；Female hormone can only lure male worm, and since male worm energy manages it multiple mating, preventive effect is often not ideal；Protein-based trapping bait can
To lure female male worm, effect is good.But the protein lure on current overseas market is pure animal protein, production cost is high.
Americal rice leaf miner (Liriomyza sativae Blanchard) falls within Diptera Agromyzidae
(Agromyzidae) Liriomyza (Liriomyza), is commonly called as vegetable leafminer, map worm, scrawly handwriting etc., is that leaf mining is caused harm
Dangerous pest.China finds most earlier than in December, 1993 first in Hainan Province Sanya, and subsequent Americal rice leaf miner is gradually northwards
Diffusion, occurs, is broken out in Shandong, Beijing within 1996 in Kunming, Yunnan occurring and damage, nineteen ninety-five for 1994 on Shanghai and Nanjing and other places
Cause disaster, occur in Henan, Shanxi within 1997, cause disaster in northeast, the Inner Mongol within 1998, in addition to Tibet, the whole nation is distributed.It
Host plant up to more than 200 is planted, and wherein vegetables are aggrieved with solanaceous vegetables (except pimento, capsicum), beans, melon, Carrot family etc.
It is most heavy.
Adult of Liriomyza Sativae Blanchard is small-sized flies, body black, and body grows 1.3~2.3 millimeters, 8.7~12.6 days service lifes, per female
70~125 pieces of all one's life oviposition, incubation rate is up to more than 90%.Larva milky is to faint yellow, long 3 millimeters, year breeding 15
~20 generations.Winter enters in heliogreenhouse, can endanger in the four seasons, mainly there is 2 hazard booms, respectively appear in 5~June and 9~
In October, year is from generation to generation up to 20 more than generation.Adult, larva can endanger, female adult pest acanthopore and feeding wherein in host's blade
And oviposition, larva potential food mesophyll or petiole after blade table is subcutaneously hatched, irregular snakelike white gallery is produced, destroys blade
Chlorophyll, influences photosynthesis, and the withered and yellow even whole strain that comes off of blade is withered when serious；Seedling stage causes to ruin seedling.Endanger serious field
Aggrieved strain 100%, blade aggrieved 70%.Phase cucurbita pepo is abounded with, after finding to endanger, mesophyll is suffered all within 7 days or so, ruins within 15 days or so
Go out.Larva and adult can also propagate some virosis by feeding.
At present, with the change of cropping system, the continuous expansion of warm winter phenomenon and open country and vegetable at protected field area is continued,
Liriomyza has become endangers one of especially big, exceptionally poverty-stricken difficult disastrous pest of prevention in various vegetables, whole year occurs more from generation to generation and weighs
Folded confusion, its spread in china is fast, and the annual generation of crop field the end of spring and the beginning of summer autumn protecting field is especially serious.The Protectorate cultivation of vegetables is vegetable
Dish liriomyza bryoniae provides good hibernacle.The worm not only can be overwintering in greenhouse, and can in canopy occurrence injury, become
The important worm sources in outdoor vegetable field.Therefore, doing the prevention in greenhouse well can effectively prevent the worm after canopy is taken off to outdoor vegetable
Diffusion harm.
Because the worm generation overlap phenomenon is serious, the resistance to the action of a drug is strong, more difficult prevention in production.At present, dive in production to America spot
The prevention of fly is still mainly based on chemical prevention, in order to solve the food-safety problem that chemical pesticide for many years is brought, Ying Li
Insect pest is controlled with bio-control method, but benefit is not also really applied and played to biological prevention in production, finds
With strong points, preventive effect is high, and to people, animal safety, it is extremely urgent without a kind of new lure of environmental pollution.
The content of the invention
For the needs of the prior art, the object of the present invention is to provide one kind be suitable for trapping trypetid and drosophila class pest,
Can the same novel microbial trapping bait caught of male and female.
For achieving the above object, technical scheme is as follows：
1. a kind of microorganism trapping bait, its active ingredient is bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) the zymotic fluid of YN2010-KC CGMCC No.5722.
2. a kind of microorganism trapping bait according to technical solution 1, the bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) zymotic fluid of YN2010-KC CGMCC No.5722 is prepared by the following method to obtain：
(1) by bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC CGMCC
After No.5722 activation, by 5~10% inoculum concentrations, by the bacillus amyloliquefaciens (Bacillus of activation
Amyloliquefaciens) YN2010-KC CGMCC No.5722 are inoculated into seed culture medium, in 35 DEG C of cultivation temperature, rotating speed
Under the conditions of 120rpm/min, 36~48h of shaking table culture, obtains primary seed solution, and the seed culture medium is LB liquid medium,
PH 7.0~7.2；
(2) 10~12% inoculum concentrations are pressed, secondary seed medium primary seed solution being transferred in seeding tank, 28~
37 DEG C, rotating speed 100rpm/min, throughput 1000L/h, tank pressure cultivates 36~48h under the conditions of being maintained at 0.02~0.03MPa,
Obtain secondary seed solution；The secondary seed medium is made of the following component of following mass fraction：Corn flour 0.3~
0.5%, analysis for soybean powder 1~1.5%, sucrose 0.2~0.5%, peptone 0.1~0.3%, fish meal 0.1~0.3%, MgSO4·
7H2O 0.02~0.04%, CaCO30.4~0.8%, (NH4)2SO40.03~0.06%, surplus is water, pH 7.0~7.2；
(3) 10~12% inoculum concentrations, fermentation medium secondary seed solution being inoculated into fermentation tank, 28~37 are pressed
DEG C, rotating speed 100rpm/min, throughput 10000L/h, tank pressure cultivate 48~72h under the conditions of being maintained at 0.02~0.03MPa,
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC CGMCC in zymotic fluid after culture
You effects Jun Shuo≤3 × 10 living of No.57229During cfu/ml, which is the bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) the zymotic fluid of YN2010-KC CGMCC No.5722, the fermentation medium and step (2) are described
Secondary seed medium is identical.
3. the microorganism trapping bait described in technical solution 1 or 2 is preventing the application of Fruit fly.
4. the microorganism trapping bait described in technical solution 1 or 2 is preventing the application of Americal rice leaf miner.
5. microorganism trapping bait according to technical solution 3 is in the application of prevention Fruit fly, the application
The microorganism trapping bait is loaded in plastic bottle, every bottle of charge weight is 50~100mL, bottle cap is screwed, in the liquid in bottle
Uniformly distributed 4 holes in the bottle wall of 1~3cm of face, the aperture in each hole is 4~6mm；Plastic bottle is hung on from the ground 0.5~1.5
At rice, plastic bottle 8~10, adjacent 5~7m of plastic bottle interval are hung per acre；One was added into plastic bottle every 20~30 days
25~50mL of the secondary microorganism trapping bait.
Compared with prior art, the invention has the advantages that：
1st, the special odor that product of the present invention gives out can lure trypetid and drosophila feeding, can male and female with catching, the action period
It is long, disposably feed intake can holding effect in two weeks, and cost is low, has stronger competitiveness.Dacus dorsalis is traped：It is more auspicious than fruit
Special drosophila attractant increase by 23.71%, than methyl eugenol increase by 18.81%.The female adult that spy's drosophila attractant more auspicious than fruit lures
Increase by 37.78%, increases by 30 times than the female adult that methyl eugenol lures.Citrus fruit fly is traped：Than methyl eugenol increase
64.43%, the citrus fruit fly female adult quantity of trapping increases by 33.25 times than methyl eugenol.Trapping to Americal rice leaf miner：It is female
Worm rate accounts for 55~60%, generates unexpected technique effect.Methyl eugenol is lured less than liriomyza bryoniae, can only be with sugar-vinegar liquid
Control, and the latter can also lure female male worm, simply total worm amount is more much lower than this product.
2nd, any chemical toxic medicament is not contained in product of the present invention, does not kill and does not kill any other non-target life
Thing, belongs to environmentally friendly fruit fly trapping bait.
3rd, product of the present invention can greatly reduce chemical pesticide dosage, so that reduction with substituted chemistry insecticide completely
Learn to farm negative effect of the medicine to natural enemy insect, no resistance to the action of a drug.
4th, product of the present invention can improve agricultural production quality and yield, and to ecological safety, ecological benefits are notable, using simple.
Bacillus amyloliquefaciens Bacillus amyloliquefaciens YN2010-KC of the present invention are in 2012
On January 12, in is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as：CGMCC), preserving number
For CGMCC No.5722, and it is detected as surviving on January 12nd, 2012.The common micro-organisms center address：Court of city of BeiJing, China
The positive institute 3 of area's North Star West Road 1, postcode:100101.
Embodiment
A kind of microorganism trapping bait provided by the invention is described in detail with reference to embodiment, but not to this hair
Bright to be construed as limiting, those skilled in the art's modified technique parameter on this basis, changes specific auxiliary material type etc. and replaces and change
It is dynamic, still belong to protection scope of the present invention.Each embodiment is conventional method without specified otherwise.
The acquisition of 1 bacillus amyloliquefaciens of embodiment (Bacillus amyloliquefaciens) YN2010-KC, mirror
Fixed and preservation
1st, the acquisition of bacillus amyloliquefaciens YN2010-KC of the present invention
1.1 material
Pick up from the soil around the Rhizosphere of Crops of Yunnan Prov Agriculture University Hou Shan farms.
It is prepared by 1.2 culture mediums
LB solid mediums and PSA culture mediums (potato sucrose agar medium) are melted, pour into sterilizing culture respectively
Ware, cooling, is made LB and PSA tablets.
1.3 it is separately cultured
Using dilution plate partition method and plate streaking separation, a strain was named YN2010- of gained will be isolated and purified
KC。
2nd, the identification of bacillus amyloliquefaciens YN2010-KC
2.1st, Morphological Identification
Take bacterial strain YN2010-KC to be cultivated on LB culture mediums, when 37 DEG C of cultures 36 are small, carry out morphologic observation identification, thalline
For rod-shaped, end circle, peritrichous, tool motility, produce oval gemma；Bacterium colony circle, weak tea are brown on LB and PSA tablets
Color, bacterium colony central color are slightly deep, protuberance, dry tack free have fold.With《Common bacteria system identification handbook》(east show pearl etc. is compiled
Write, Science Press, 2001) described in bacillus morphological feature it is basically identical, tentatively judge bacterial strain YN2010-KC
Belong to bacillus (Bacillus spp.).
2.2nd, Physiology and biochemistry is identified
Bacterial strain Gram's staining, spore staining, Starch Hydrolysis, glucose utilization, citrate utilization, V-P reactions, contact
Enzyme reaction, nitrate reduction, gelatin liquefaction etc. are positive.
3rd, the preservation of bacillus amyloliquefaciens YN2010-KC
Show that the bacterial strain of separated acquisition is bacillus amyloliquefaciens (Bacillus through above-mentioned identify
Amyloliquefaciens), its code name is YN2010-KC, the bacillus amyloliquefaciens Bacillus
Amyloliquefaciens YN2010-KC were deposited in China Committee for Culture Collection of Microorganisms on January 12nd, 2012
Common micro-organisms center is (referred to as：CGMCC), preserving number is CGMCC No.5722, and is detected as surviving on January 12nd, 2012.
The common micro-organisms center address：City of BeiJing, China Chaoyang District North Star West Road 1 institute 3, postcode:100101.
The preparation method of the microorganism trapping bait of the present invention of embodiment 2, comprises the following steps：
(1) by bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC of -80 DEG C of preservations
CGMCC No.5722 are seeded on LB liquid medium, 35 DEG C of culture 24h, obtain the bacillus amyloliquefaciens (Bacillus of activation
Amyloliquefaciens) YN2010-KC CGMCC No.5722, by 10% inoculum concentration, by the solution starch gemma of activation
Bacillus (Bacillus amyloliquefaciens) YN2010-KC CGMCC No.5722 are inoculated into seed culture medium, are training
Under the conditions of supporting 35 DEG C of temperature, rotating speed 120rpm/min, shaking table culture 36h obtains primary seed solution, and the seed culture medium is liquid
Body LB culture mediums, pH 7.0~7.2；
(2) 10% inoculum concentration is pressed, secondary seed medium primary seed solution being transferred in seeding tank, at 35 DEG C, turns
Fast 100rpm/min, throughput 1000L/h, tank pressure cultivate 36~48h under the conditions of being maintained at 0.02~0.03MPa, obtain two
Level seed liquor；Obtain secondary seed solution；The secondary seed medium is made of the following component of following mass fraction：Corn flour
0.5%, analysis for soybean powder 1%, sucrose 0.5%, peptone 0.3%, fish meal 0.1%, MgSO4·7H2O 0.02%, CaCO3
0.4%, (NH4)2SO40.03%, surplus is water, pH 7.0~7.2；The secondary seed medium is in 121 DEG C of sterilizings
30min, waits to be cooled to 30 DEG C, accesses primary seed solution；
(3) 10% inoculum concentration is pressed, fermentation medium secondary seed solution being inoculated into fermentation tank, at 35 DEG C, rotating speed
100rpm/min, throughput 10000L/h, tank pressure cultivate 48~72h under the conditions of being maintained at 0.02~0.03MPa, after culture
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC CGMCC No.5722 have in zymotic fluid
Effect Jun Shuo≤3 × 10 living9During cfu/ml, zymotic fluid is packed with medicinal plastic bottle, as the microorganism trapping bait, the hair
Ferment culture medium is identical with step (2) described secondary seed medium.
Microorganism trapping bait of the present invention is prepared by the preparation method of the microorganism trapping bait described in embodiment 2.
The contrast test that the microorganism trapping bait of the present invention of embodiment 3 traps dacus dorsalis with other lures
In Yuanjiang county citrus orchard carry out trap effect experiment, while with " fruit Rui Te " drosophila attractants, (belong to egg
It is white bait formulation, commercially available), methyl eugenol (attribute lure, commercially available) and water (blank control) contrasted.Microorganism trapping of the present invention
50ml/ bottles of agent dosage, auspicious special 50ml/ bottles of the drosophila attractant dosage of fruit, 3ml/ bottles of methyl eugenol dosage.
A kind of above-mentioned attractant of each plastic bottle, screws bottle cap, is being evenly distributed with 4 in the bottle wall of liquid level 2cm in bottle
Hole, the aperture in each hole is 5mm.Field adaptability is carried out in noon on May 16th, 2015, plastic bottle is hung on from citrus
At 0.5-1.5 meters.Dacus dorsalis quantity (head) is lured to be shown in Table 1 two days later.As it can be seen that microorganism trapping bait of the present invention lures borer population amount
The auspicious special drosophila attractant of fruit and methyl eugenol are above, and female adult rate is up to 51.67%.Microorganism trapping bait of the present invention lures worm
Quantity special drosophila attractant increase by 23.71% more auspicious than fruit, than methyl eugenol increase by 18.81%.Microorganism trapping bait of the present invention
The female adult increase by 37.78% that spy's drosophila attractant more auspicious than fruit lures, increases by 30 times than the female adult that methyl eugenol lures.
The microorganism trapping bait of the present invention of table 1 is to dacus dorsalis trapping experiment
The microorganism trapping bait of the present invention of embodiment 4 is to citrus fruit trypetid trapping experiments
Fruit fly trapping experiments, test reagent are carried out on Yunnan Province Hekou County Nanxi farm：Microorganism trapping bait of the present invention.
The plastic bottle and same as Example 3,50ml/ bottles of the microorganism trapping bait dosage of the present invention, July 5 in 2014 of operation of installed reagents
The plastic bottle of day noon equipped with reagent is hung at 0.5-1.5 meters from citrus, investigates each bottle within 1 day, 2 days and 3 days after feeding intake certainly
Middle worm amount is shown in Table 2, it is seen then that trapping bait of the present invention fermentation produces the compounds such as volatility is protein-based and lures fruit fly to eat, and imitates
Fruit is notable, and male and female lure together.
Table 2 trapping of microorganism trapping bait fruit fly (citrus fruit fly, small trypetid, Bactrocera correcta, melonfly of the present invention
Deng) effect
Contrast test of the microorganism trapping bait of the present invention of embodiment 5 to citrus fruit fly prevention effect
Trapping citrus fruit fly experiment is carried out in the citrus orchard of Mengzi County, Yunnan Province, while is carried out pair with methyl eugenol
Than.50ml/ bottles of microorganism trapping bait dosage of the present invention, 50ml/ bottles of methyl eugenol dosage.
It is same as Example 3 to fill plastic bottle and the operation of each reagent, in noon on December 4th, 2014 by the modeling equipped with reagent
Material bottle is hung at 0.5-1.5 meter from citrus, is investigated worm amount in each bottle and is shown in Table 3 within 1 day, 2 days and 3 days after feeding intake certainly, it is seen then that use
Microorganism trapping bait of the present invention fermentation produces the compounds such as volatility is protein-based and lures citrus fruit fly to eat, and lures except first day
Worm amount is less than outside gyplure methyl eugenol, and the effect of the big trypetid of trapping is superior to gyplure methyl eugenol in other time,
And male and female lure together.In three days, microorganism trapping bait trapping citrus fruit fly quantity of the present invention is than methyl eugenol increase
64.43%, the citrus fruit fly female adult quantity of trapping increases by 33.25 times than methyl eugenol.
The effect of the microorganism trapping bait trapping citrus fruit fly of the present invention of table 3
The microorganism trapping bait of the present invention of embodiment 6 lures worm contrast test with yellow plate field
Trap effect experiment is carried out on the tomato vegetable base of Mengzi County, Yunnan Province, is carried out with field and 30 × 30cm Huangs plate
Contrast.Each bottled 50ml of yellow plastic microorganism trapping baits of the present invention, screw bottle cap, in the bottle in bottle at liquid level 2cm
Uniformly distributed 4 holes on wall, the aperture in each hole is 5mm.Field adaptability is carried out in noon on June 16th, 2015, plastic bottle is hanged
0.5~0.8 meter away from ground height is hung on, hangs plastic bottle 8, adjacent 5~10m of plastic bottle interval per acre；In same field, Jiang Huangban
Hang on high away from ground 0.5-0.8 meters, hang yellow plate 8, adjacent yellow 5~10m of plate interval per acre.It is every to lure two days later to America
Liriomyza bryoniae quantity (head) is shown in Table 4.Borer population amount is lured to be above yellow plate daily as it can be seen that microorganism trapping bait of the present invention is average, in 8 days,
Microorganism trapping bait of the present invention lures worm 1988, yellow trap 379, is 5.2 times of yellow trap amount.
The contrast test that the microorganism trapping bait of the present invention of table 4 and yellow plate lure Americal rice leaf miner
Implement 7 microorganism trapping baits of the present invention to Americal rice leaf miner male and female quantity trapping experiments
In Yunnan Province Jiangchuan County Niu Mo villages, Vegetable Base carries out cowpea Americal rice leaf miner trapping experiments, and the 50ml present invention is micro-
Organism trapping agent is directly added into outer wall and posts in yellow adhesive tape plastic bottle, screws bottle cap, in the bottle wall in bottle at liquid level 2cm
Upper to be evenly distributed with 4 holes, the aperture in each hole is 5mm.Hung in 2 months 3 noons in 2015 apart from 0.5~0.8 meter of ground height,
Investigate worm amount in each bottle and be shown in Table 5 within 1 day, 2 days and 3 days from after feeding intake, it is seen then that waved with what microorganism trapping bait of the present invention fermentation produced
The hair property compound such as protein-based lures Americal rice leaf miner to eat, significant effect, and male and female are with luring, and female adult rate is up to 55~60%.
The microorganism trapping bait of the present invention of table 5 catches male and female Americal rice leaf miner statistics
Claims (3)
1. a kind of microorganism trapping bait is to solve in the application of prevention Fruit fly, the active ingredient of the microorganism trapping bait
The zymotic fluid of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC CGMCC No.5722.
2. application according to claim 1, it is characterised in that：The application is to load the microorganism trapping bait to mould
Expect in bottle, every bottle of charge weight is 50~100mL, screws bottle cap, is being evenly distributed with 4 in the bottle wall of 1~3cm of liquid level in bottle
Hole, the aperture in each hole is 4~6mm；Plastic bottle is hung on from the ground at 0.5~1.5 meter, hang per acre plastic bottle 8~
10, adjacent 5~7m of plastic bottle interval；Added once into plastic bottle every 20~30 days the microorganism trapping bait 25~
50mL。
3. a kind of microorganism trapping bait is to solve in the application of prevention Americal rice leaf miner, the active ingredient of the microorganism trapping bait
The zymotic fluid of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC CGMCC No.5722.
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| PCT/CN2016/110474 WO2017107872A1 (en) | 2015-12-21 | 2016-12-16 | Microbial attractant, and use |
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| CN105410032B (en) * | 2015-12-21 | 2018-04-24 | 云南省微生物发酵工程研究中心有限公司 | A kind of microorganism trapping bait and application |
| GB2551121B (en) * | 2016-06-02 | 2021-01-06 | Fungialert Ltd | In situ detection of pathogens |
| CN107593620B (en) * | 2017-09-30 | 2024-03-22 | 河南农业大学 | Biological brick for growth and propagation of hermetia illucens and manufacturing and using methods thereof |
| CN108377819A (en) * | 2018-02-05 | 2018-08-10 | 云南省烟草公司普洱市公司 | A method of prevention tobacco black shank |
| CN109320368A (en) * | 2018-11-08 | 2019-02-12 | 北京泰克美高新技术有限公司 | The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae |
| CN111513083A (en) * | 2019-04-22 | 2020-08-11 | 泰山医学院 | Novel housefly specific long-acting bacterial attractant and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN105385638A (en) * | 2015-12-21 | 2016-03-09 | 云南省微生物发酵工程研究中心有限公司 | Microbial phosphorus-dissolving preparation and preparation method and application thereof |
| CN105494445A (en) * | 2015-12-21 | 2016-04-20 | 云南农业大学 | Preparation for preventing and controlling pepper blight and application |
| CN105519582A (en) * | 2015-12-21 | 2016-04-27 | 云南省微生物发酵工程研究中心有限公司 | Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2239548B1 (en) * | 2004-03-11 | 2007-02-01 | Universidad Politecnica De Valencia | A FOOD BIT WITH SPECIFIC ATTRACTOR TO FIGHT TEFRITIDED INSECTS. |
| CN101584346A (en) * | 2008-11-19 | 2009-11-25 | 南京林业大学 | Method for preparing biological preparation for efficiently poisoning plant nematodes |
| CN101589728B (en) * | 2009-06-30 | 2011-12-14 | 福建农林大学 | Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof |
| CN102154156B (en) * | 2010-12-28 | 2013-02-13 | 南京农业大学 | Antagonists capable of preventing and controlling tomato bacterial wilt or knot nematode and microbial organic fertilizer thereof |
| CN102754665B (en) * | 2012-07-17 | 2014-05-14 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
| CN102952768B (en) * | 2012-11-05 | 2015-03-11 | 云南省烟草农业科学研究院 | Bacillus, bacterial agent, preparation method and applications thereof |
| CN104498040B (en) * | 2014-11-10 | 2018-08-31 | 杨凌绿都生物科技有限公司 | Application of the bacillus amyloliquefaciens as soil conditioner |
| CN104371959B (en) * | 2014-11-18 | 2017-12-05 | 吴山功 | Biological deodorizing fly eradication composite bacteria agent and its application |
| CN105410032B (en) * | 2015-12-21 | 2018-04-24 | 云南省微生物发酵工程研究中心有限公司 | A kind of microorganism trapping bait and application |
-
2015
- 2015-12-21 CN CN201510958305.8A patent/CN105410032B/en active Active
-
2016
- 2016-12-16 WO PCT/CN2016/110474 patent/WO2017107872A1/en active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN105385638A (en) * | 2015-12-21 | 2016-03-09 | 云南省微生物发酵工程研究中心有限公司 | Microbial phosphorus-dissolving preparation and preparation method and application thereof |
| CN105494445A (en) * | 2015-12-21 | 2016-04-20 | 云南农业大学 | Preparation for preventing and controlling pepper blight and application |
| CN105519582A (en) * | 2015-12-21 | 2016-04-27 | 云南省微生物发酵工程研究中心有限公司 | Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| "一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究";权春善 等，;《微生物学报》;20060204;第46卷(第1期);第7-12页 * |
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Denomination of invention: A Microbial Trapping Agent and Its Application Effective date of registration: 20231120 Granted publication date: 20180424 Pledgee: Kunming Dongfeng Sub branch of Bank of China Ltd. Pledgor: MICROBIAL FERMENTATION ENGINEERING RESEARCH CENTER Co.,Ltd. OF YUNNAN PROVINCE Registration number: Y2023530000066 |