CN102754665B - Application of hansenula anomala in preparing citrus fruit fly bait - Google Patents
Application of hansenula anomala in preparing citrus fruit fly bait Download PDFInfo
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- CN102754665B CN102754665B CN201210245822.7A CN201210245822A CN102754665B CN 102754665 B CN102754665 B CN 102754665B CN 201210245822 A CN201210245822 A CN 201210245822A CN 102754665 B CN102754665 B CN 102754665B
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Abstract
The invention discloses an application of hansenula anomala in preparing citrus fruit fly bait. The invention also discloses citrus fruit fly bait which consists of hansenula anomala hydrolysis fermentation broth and auxiliary bait, wherein the auxiliary bait includes polypeptone, brown sugar, table vinegar or ammonium acetate, preservative and water composition or sweet and sour liquid. The citrus fruit fly bait disclosed by the invention realizes a good effect on inducing bactrocera minax and bactrocera dorsalis as well as a good effect on inducing both the females and males of the bactrocera minax and bactrocera dorsalis, is safe and non-toxic and harmless to human bodies and other beneficial insects, and avoids the residues.
Description
Technical field
The invention belongs to citrus pest prevention and control field, be specifically related to a kind of Hansenula anomala (
hansenula anomala) lure the application in bait formulation preparing oranges and tangerines trypetid class, the invention still further relates to oranges and tangerines trypetid class and lure bait formulation.
Background technology
Citrus fruit fly
bactrocera (Tetradacus) minax, have another name called the large trypetid of tangerine, belong to diptera, Tephritidae, Dacinae, is commonly called as " mandarin orange maggot ", is the crushing insect of one of oranges and tangerines.There is a generation for 1 year in citrus fruit fly, killed fruit is called maggot fruit or maggot mandarin orange.Citrus fruit fly survives the winter in soil with pupa, generally starts to sprout wings in mid or late May and is unearthed.After adult sexual maturity, on the tangerine that flies to, mating is laid eggs, and female Adult worms producting eggs, in the endodermis of fruit, has ovum 13-17 grain in general each oviporous orifice, can reach at most 35.From hard to soft and flexible, underdone first yellow, majority comes off, rots killed fruit.
Because citrus fruit fly Adult worms producting eggs is in fruit, larva is damage in citrus fruit, is difficult to reach insecticidal effect so spray medicament, and chemical pesticide is poisonous to people and other beneficial insects, easily produces the pesticide resistance of residue of pesticide and insect.Adult baiting technique is one of important step of the comprehensive prevention and control of citrus fruit fly, and at present, the bait formulation of luring of citrus fruit fly mainly contains sweet and sour mixing bait formulation and albumen bait formulation, limited to citrus fruit fly attractant effect.
Dacus dorsalis (
bactrocera dorsailshendel), have another name called citrus fruit fly, belong to diptera Tephritidae Bactrocera, have another name called orient fruit fly, harm carambola, guava, oranges and tangerines, mango, banana, bajiao banana, grape and 250 various vegetables and the fruit such as melon, be a kind of global quarantine pest insect, the important agricultural pest of Ye Shi China simultaneously.Approximately there is 3-6 generation in 1 year in China in citrus fruit fly, without strict During Overwintering Period, generation overlap, causes the various worm states in areal and deposit, and brings a lot of difficulties to the integrated control of citrus fruit fly.
The same with citrus fruit fly, citrus fruit fly is to the harm of fruit mainly by Adult worms producting eggs, and the larva after hatching, at the inner harm of fruit pulp, is caused fruit abscission, rots, and has a strong impact on output and the quality of fruit, brings huge loss to agricultural production.Citrus fruit fly inducing substance mainly contains at present: (1) protein hydrolysate class.Such material has effect to male and female individuality, and the scope of trapping is wider.(2) plant resource class.This class Material Source, in the secondary metabolite of plant or plant, generally male and female individuality is all had to attracting action, but effect is limited.(3) chemosynthesis class.Wherein methyl eugenol is current most widely used attractant, but only lures male worm.
Summary of the invention
The object of the present invention is to provide a kind of Hansenula anomala to lure the application in bait formulation preparing oranges and tangerines trypetid class, another object of the present invention is to provide two kinds of good oranges and tangerines trypetid classes of attractant effect to lure bait formulation.
Above-mentioned purpose is achieved through the following technical solutions:
Hansenula anomala is lured the application in bait formulation preparing oranges and tangerines trypetid class.
Described Hansenula anomala, belongs to Ascomycetes, Endomycetale, and Mucoraceae, Hansenula anomala belongs to.
Rounded, milky that Hansenula anomala falls, yeast sample, tarnish are slightly swelled in smooth, center, and surface has that sensation of dryness, edge are slightly not whole, surrounding is shallow corrugated sample bacterium colony, has the protuberance of the radiation ridge shape to periphery from bacterium colony center.Its main application, for producing fat, increases alcoholic drink fragrance, can utilize alcohol for carbon source forms epithelium in beverage surface, is the harmful bacteria that drinks is brewageed, and can cause the rotten of the food such as syrup, jam, fruit juice.Because producing ethyl acetate, also can be used for the flavouring of food.
A kind of oranges and tangerines trypetid class provided by the invention is lured bait formulation, is made up of Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation, and described auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 5-20%;
Brown sugar 8-20%;
Vinegar 10-20% or ammonium acetate 1.2-3%;
Preservative 0.1-0.3%;
Water surplus,
Wherein: preservative is Sodium Benzoate or methyl p-hydroxybenzoate,
The volume ratio of described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:0.5-2.
Preferably, the volume ratio of described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:1.
Preferably, described auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 15%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Water surplus.
Another kind of oranges and tangerines trypetid class provided by the invention is lured bait formulation, is made up of Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation, and its volume ratio is 1:1, and described auxiliary bait formulation is sugar-vinegar liquid, and in described sugar-vinegar liquid, the mass ratio of sugar, vinegar, water is 1:1:8.
The invention has the beneficial effects as follows:
Oranges and tangerines trypetid class of the present invention lures bait formulation good to the attractant effect of citrus fruit fly and citrus fruit fly, and female worm and male worm to citrus fruit fly and citrus fruit fly all have good attractant effect, and safety non-toxic, noresidue, harmless to people and other useful insect.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
A kind of oranges and tangerines trypetid class is lured bait formulation, is made up of Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation, and the volume ratio of described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:1.
Described auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 15%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Water surplus.
Preparation method comprises the following steps:
1, material is prepared
Hansenula anomala strain is purchased from Chinese agriculture microorganism fungus kind preservation administrative center (Agricultural Culture Collection of China, english abbreviation ACCC), multivalence peptone is purchased from Qingdao GaoKeYuan Hai Bo Bioisystech Co., Ltd, brown sugar, vinegar, ammonium acetate, preservative are commercially available prod, and wherein vinegar is light-coloured vinegar or mature vinegar or mature vinegar.
YPD medium component (quality %): 1% yeast soaks powder (Beijing bispin microbiological culture media products factory), 2% tryptone (Beijing bispin microbiological culture media products factory), 2% glucose, if solid culture medium processed, adds 2% agar powder (Biosharp company) again.Compound method:
(1) dissolve 10g yeast and soak powder, 20g tryptone is in 900ml water, as flat board processed adds 20g agar powder.
(2) 121 ℃ of high pressure, 20min.
(3) add 100ml 10% glucose (adding after the sterilizing of glucose sugar juice).
Note: glucose, yeast soaks powder, and at high temperature may there is chemical reaction after mixing in tryptone solution, cause medium component to change, so mix after will distinguishing sterilizing again.
2, the renewal cultivation of bacterial strain
(1) draw the suitable liquid nutrient medium of 0.3-0.5ml with aseptic straw, splash in ampoul tube, light shaking, dissolves freeze-drying thalline and is suspension.
(2) draw thallus suspension liquid, transplant on suitable agar slant or plating medium, then at the temperature of suggestion, cultivate.Advise an ampoul tube bacterial classification 2 to 3 inclined-planes of only transferring.All bacterium powder must disposable being finished.
Points for attention:
(1), before actication of culture, ampoul tube is kept under the environment of 6-10 ℃.
(2) some bacterial classification is after freeze drying is preserved, and period of delay is longer, needs the double subculture normal growth that cultivates one's ability.
3, the preparation of fermented liquid
Prepare shaking table cultivation fermentation medium, with the preparation of 250ml conical flask, every bottle of specification 200ml medium, 121 ℃ of sterilizing 20min of high pressure.With sterilized oese picking 2-3 ring thalline from all right flat board of growth or slant medium, be inoculated in fermentation medium, concussion is evenly.Finished conical flask is put into constant-temperature table, and 220rpm, cultivates 3 days for 30 ℃, takes out 4 ℃ of refrigerations for subsequent use.
4, oranges and tangerines trypetid class is lured the preparation of bait formulation
(1) prepare the hydrolysed ferment liquid of Hansenula anomala: hydrolysising condition is pH value 6.0,50 ℃ of hydrolysis temperatures, hydrolysis time 18h, the enzyme concentration 2.0mg/mL of papain.
(2) the auxiliary bait formulation of preparation: get multivalence peptone, brown sugar, ammonium acetate, methyl p-hydroxybenzoate, add water to after the full dose of formula, fully stirring and dissolving mixes.
(3) take Hansenula anomala hydrolysed ferment liquid as matrix, add auxiliary bait formulation, mix.
Embodiment 2
A kind of oranges and tangerines trypetid class is lured bait formulation, is made up of Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation, and the volume ratio of described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:0.5.
Described auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 20%;
Brown sugar 8%;
Vinegar 10%;
Sodium Benzoate 0.1%;
Water surplus.
Preparation method is with embodiment 1.
Embodiment 3
A kind of oranges and tangerines trypetid class is lured bait formulation, is made up of Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation, and the volume ratio of described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:2.
Described auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 5%;
Brown sugar 20%;
Vinegar 20%;
Sodium Benzoate 0.3%;
Water surplus.
Preparation method is with embodiment 1.
Embodiment 4 oranges and tangerines trypetid classes lure bait formulation to measure the field attractant effect of citrus fruit fly
1, test grouping:
(1) Hansenula anomala hydrolysed ferment liquid
(2) albumen bait formulation (the auxiliary bait formulation in embodiment 1)
(3) the oranges and tangerines trypetid class of embodiment 1,2,3 is lured bait formulation (Hansenula anomala hydrolysed ferment liquid+albumen bait formulation)
(4) Hansenula anomala hydrolysed ferment liquid+sugar-vinegar liquid (volume ratio 1:1, in sugar-vinegar liquid, the mass ratio of sugar, vinegar, water is 1:1:8)
(5) the auspicious special fruit fly attractant dilution of fruit (be purchased from Hubei Grea Biotech Co., Ltd., dilution ratio is 1:2)
(6) sugar-vinegar liquid (mass ratio of sugar, vinegar, water is 1:1:8).
2, test method: get respectively each processing 50ml and put into yellow trapping tank, hang in the shade liftoff 1.5m place, mandarin tree tree crown middle part, 3 repetitions of each processing with iron wire.The random evenly interval 20m of different disposal is suspended in experiment orangery.Continuous Observation 3 days, the citrus fruit fly male and female number that record is lured.Contrast with sugar-vinegar liquid, calculate according to the following formula the lure coefficient of each bait formulation to citrus fruit fly.
The borer population (head) of luring the borer population (head) that coefficient=bait formulation to be measured lures/contrast sugar-vinegar liquid to lure
3, result of the test
Table 1: different disposal bait formulation is to citrus fruit fly attractant effect
Note: in table, numeral is " mean+SD ", is respectively listed as significant difference in the level that is illustrated in P<0.05 between different lowercases.
The attractant effect significant difference (table 1) of the bait formulation of different formulations to citrus fruit fly.What attractant effect was best is Hansenula anomala hydrolysed ferment liquid+albumen bait formulation formula, is 3.52 times of control group sugar-vinegar liquid, and female male worm is all had to good attractant effect.Meanwhile, Hansenula anomala hydrolysed ferment liquid+sugar-vinegar liquid is better than the attractant effect of independent Hansenula anomala zymotic fluid, and significant difference illustrates that sugar-vinegar liquid has synergistic effect.Albumen bait formulation, Hansenula anomala hydrolysed ferment liquid+sugar-vinegar liquid and Hansenula anomala hydrolysed ferment liquid+albumen bait formulation are all good than the attractant effect of fruit Rui Te, significant difference.
Embodiment 5 oranges and tangerines trypetid classes lure bait formulation to measure the field attractant effect of dacus dorsalis
1, test grouping:
(1) Hansenula anomala hydrolysed ferment liquid
(2) Hansenula anomala hydrolysed ferment liquid+sugar-vinegar liquid (volume ratio 1:1, in sugar-vinegar liquid, the mass ratio of sugar, vinegar, water is 1:1:8)
(3) Hansenula anomala hydrolysed ferment liquid+methyl eugenol (methyl eugenol volume ratio 1%)
(4) the oranges and tangerines trypetid class of embodiment 1,2,3 is lured bait formulation (Hansenula anomala hydrolysed ferment liquid+albumen bait formulation)
(5) albumen bait formulation (the auxiliary bait formulation in embodiment 1)
(6) methyl eugenol paraffin mixed liquor (methyl eugenol volume fraction 1%)
(7) sugar-vinegar liquid (mass ratio of sugar, vinegar, water is 1:1:8).
2, test method: measure respectively the lure of 5ml with pipettor, make and lure core with absorbent cotton absorption.Trapper is purchased from Ningbo NewCon Co., Ltd..Trapper is hung on to tangerine tree crown middle part shady spot with iron wire, 3 repetitions of each processing, the even interval of different disposal hangs.Within every 2 days, record experimental data, the male and female citrus fruit fly quantity that statistics is lured, and change lure, with sugar-vinegar liquid in contrast, calculate according to the following formula the lure coefficient of each bait formulation to citrus fruit fly.
The borer population (head) of luring the borer population (head) that coefficient=bait formulation to be measured lures/contrast sugar-vinegar liquid to lure
3, result of the test
Table 2: different disposal bait formulation is to dacus dorsalis attractant effect
Note: in table, numeral is " mean+SD ", is respectively listed as significant difference in the level that is illustrated in P<0.05 between different lowercases.
The bait formulation of different formulations is to citrus fruit fly attractant effect significant difference.It is contrast 2.7 times of sugar-vinegar liquid that oranges and tangerines trypetid class of the present invention is lured bait formulation attractant effect, is also better than albumen bait formulation formula simultaneously.Although Hansenula anomala hydrolysed ferment liquid+methyl eugenol lures maximum borer populations, but the borer population there was no significant difference of luring bait formulation to lure with this experiment oranges and tangerines trypetid class, and bad for female attractant effect, independent methyl eugenol paraffin mixed liquor can only lure male worm, considers oranges and tangerines trypetid class and lures the attractant effect of bait formulation best.And independent Hansenula anomala hydrolysed ferment liquid exists significant difference with the attractant effect of the Hansenula anomala hydrolysed ferment liquid that has added sugar-vinegar liquid, illustrate that sugar-vinegar liquid has synergistic effect to it.
Claims (3)
1. oranges and tangerines trypetid class is lured a bait formulation, it is characterized in that being made up of Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation, and described auxiliary bait formulation is made up of the composition of following mass percent:
Wherein: preservative is Sodium Benzoate or methyl p-hydroxybenzoate,
The volume ratio of described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:0.5-2.
2. oranges and tangerines trypetid class as claimed in claim 1 is lured bait formulation, and the volume ratio that it is characterized in that described Hansenula anomala hydrolysed ferment liquid and auxiliary bait formulation is 1:1.
3. oranges and tangerines trypetid class as claimed in claim 1 or 2 is lured bait formulation, it is characterized in that described auxiliary bait formulation is made up of the composition of following mass percent:
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| CN103740603B (en) * | 2014-01-26 | 2015-08-05 | 北京绿环国际科技有限公司 | A kind of Abnormal pichia anomala and application thereof |
| CN104542720A (en) * | 2014-12-19 | 2015-04-29 | 邓仁双 | Method for biologically controlling Bactrocera minax (Enderlein) |
| CN104892090A (en) * | 2015-04-30 | 2015-09-09 | 河北双星种业有限公司 | Multifunctional insecticidal and antibacterial nutrient solution and use method thereof |
| CN105410032B (en) * | 2015-12-21 | 2018-04-24 | 云南省微生物发酵工程研究中心有限公司 | A kind of microorganism trapping bait and application |
| CN107821392B (en) * | 2017-10-11 | 2020-09-29 | 华中农业大学 | Long-acting tablet with high attractability to citrus fruit flies and preparation method thereof |
| CN111449088B (en) * | 2019-04-22 | 2021-11-09 | 山东第一医科大学(山东省医学科学院) | Novel lucilia sericata specific long-acting bacterial attractant and preparation method thereof |
| CN112602706B (en) * | 2021-01-09 | 2022-02-01 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
| CN115444007A (en) * | 2022-09-14 | 2022-12-09 | 漳州市美创生物科技有限公司 | Bactrocera dorsalis attractant and preparation method and application thereof |
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