CN102754665A - Application of hansenula anomala in preparing citrus fruit fly bait - Google Patents
Application of hansenula anomala in preparing citrus fruit fly bait Download PDFInfo
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- CN102754665A CN102754665A CN2012102458227A CN201210245822A CN102754665A CN 102754665 A CN102754665 A CN 102754665A CN 2012102458227 A CN2012102458227 A CN 2012102458227A CN 201210245822 A CN201210245822 A CN 201210245822A CN 102754665 A CN102754665 A CN 102754665A
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Abstract
The invention discloses an application of hansenula anomala in preparing citrus fruit fly bait. The invention also discloses citrus fruit fly bait which consists of hansenula anomala hydrolysis fermentation broth and auxiliary bait, wherein the auxiliary bait includes polypeptone, brown sugar, table vinegar or ammonium acetate, preservative and water composition or sweet and sour liquid. The citrus fruit fly bait disclosed by the invention realizes a good effect on inducing bactrocera minax and bactrocera dorsalis as well as a good effect on inducing both the females and males of the bactrocera minax and bactrocera dorsalis, is safe and non-toxic and harmless to human bodies and other beneficial insects, and avoids the residues.
Description
Technical field
The invention belongs to the oranges and tangerines field of pest control, be specifically related to a kind of unusual Hansenula yeast bacterium (
Hansenula anomala) lure the application in the bait formulation in preparation oranges and tangerines trypetid class, the invention still further relates to oranges and tangerines trypetid class and lure bait formulation.
Background technology
Citrus fruit fly
Bactrocera (Tetradacus) minax, have another name called the big trypetid of tangerine, belong to diptera, Tephritidae, few hair on the neck trypetid subfamily is commonly called as " mandarin orange maggot ", is a kind of crushing insect of oranges and tangerines.A generation took place in 1 year in citrus fruit fly, and killed fruit is called maggot fruit or maggot mandarin orange.Citrus fruit fly survives the winter in soil with pupa, generally begins to sprout wings in mid or late May and is unearthed.After the adult sexual maturity, mating is laid eggs on the tangerine that flies to, and female adult worm is laid eggs in the endodermis of fruit, and ovum 13-17 grain is generally arranged in each oviporous orifice, can reach 35 at most.Killed fruit from hard to soft and flexible, underdone yellow earlier, majority comes off, rots.
Because the citrus fruit fly adult lays eggs in fruit, larva eats into food in citrus fruit causes harm, and is difficult to reach insecticidal effect so spray medicament, and chemical pesticide is poisonous with other beneficial insects to the people, is prone to produce the pesticide resistance of residue of pesticide and insect.The adult baiting technique is one of important step of the comprehensive prevention and control of citrus fruit fly, and at present, the bait formulation of luring of citrus fruit fly mainly contains sweet and sour mixing bait formulation and albumen bait formulation, and is limited to the citrus fruit fly attractant effect.
Dacus dorsalis (
Bactrocera dorsailsHendel); Have another name called citrus fruit fly, belong to diptera Tephritidae fruit fly and belong to, have another name called orient fruit fly; Harm carambola, guava, oranges and tangerines, mango, banana; Bajiao banana, grape and 250 various vegetables and fruit such as melon are a kind of global quarantine pest insects, also are the important agricultural property pests of China simultaneously.In 3-6 generation, approximately take place in China 1 year in citrus fruit fly, do not have the strict process of surviving the winter, and generation overlap causes the various worm attitudes of areal and deposits, and brings a lot of difficulties for the integrated control of citrus fruit fly.
The same with citrus fruit fly, citrus fruit fly is mainly laid eggs through adult to the harm of fruit, and the larva after the hatching is caused fruit abscission, rots at the inner harm of fruit pulp, has a strong impact on the output and the quality of fruit, brings tremendous loss to agricultural production.The citrus fruit fly inducing substance mainly contains at present: (1) protein hydrolysate class.Such material all has effect to the male and female individuality, and the scope of trapping is wider.(2) plant resource class.This type material derives from the secondary metabolite of plant or plant, generally the male and female individuality is all had attracting action, but effect is limited.(3) chemosynthesis class.Wherein methyl eugenol is present most widely used attractant, but only lures male worm.
Summary of the invention
The object of the present invention is to provide a kind of unusual Hansenula yeast bacterium to lure the application in the bait formulation, another object of the present invention is to provide two kinds of good oranges and tangerines trypetid classes of attractant effect to lure bait formulation in preparation oranges and tangerines trypetid class.
Above-mentioned purpose realizes through following technical scheme:
Unusual Hansenula yeast bacterium is lured the application in the bait formulation in preparation oranges and tangerines trypetid class.
Described unusual Hansenula yeast bacterium belongs to Ascomycetes, Endomycetale, Mucoraceae, unusual Hansenula.
Unusual rounded, the milky of Hansenula yeast bacterium colony, smooth, center are swelled yeast appearance, tarnish slightly, the surface have sensation of dryness, edge slightly whole, be shallow corrugated appearance bacterium colony all around, have from the bacterium colony center to the radiation ridge shape protuberance of periphery.Its main application increases alcoholic drink fragrance for producing fat, can utilize alcohol to form epithelium for carbon source in beverage surface, is harmful bacterium that drinks is brewageed, and can cause the rotten of food such as syrup, jam, fruit juice.Because of producing ethyl acetate, also can be used for the flavouring of food.
A kind of oranges and tangerines trypetid class provided by the invention is lured bait formulation, is made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 5-20%;
Brown sugar 8-20%;
Vinegar 10-20% or ammonium acetate 1.2-3%;
Preservative 0.1-0.3%;
Water surplus,
Wherein: preservative is Sodium Benzoate or methyl p-hydroxybenzoate,
The volume ratio of said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:0.5-2.
Preferably, the volume ratio of said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:1.
Preferably, said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 15%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Water surplus.
Another kind of oranges and tangerines trypetid class provided by the invention is lured bait formulation, is made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and its volume ratio is 1:1, and said auxiliary bait formulation is a sugar-vinegar liquid, and the mass ratio of sugar, vinegar, water is 1:1:8 in the said sugar-vinegar liquid.
The invention has the beneficial effects as follows:
Oranges and tangerines trypetid class of the present invention lures bait formulation good to the attractant effect of citrus fruit fly and citrus fruit fly; And female worm and male worm to citrus fruit fly and citrus fruit fly all have good attractant effect, and safety non-toxic, noresidue, harmless to people and other useful insect.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
A kind of oranges and tangerines trypetid class is lured bait formulation, is made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and the volume ratio of said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:1.
Said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 15%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Water surplus.
The preparation method may further comprise the steps:
1, material is prepared
Unusual Hansenula yeast bacterial strain is purchased (the Agricultural Culture Collection of China in Chinese agriculture microorganism fungus kind preservation administrative center; English abbreviation ACCC), the multivalence peptone is available from Qingdao GaoKeYuan Hai Bo Bioisystech Co., Ltd; Brown sugar, vinegar, ammonium acetate, preservative are the commercially available prod, and wherein vinegar is light-coloured vinegar or mature vinegar or mature vinegar.
YPD medium component (quality %): 1% yeast soaks powder (Beijing bispin microbiological culture media products factory), 2% tryptone (Beijing bispin microbiological culture media products factory), 2% glucose; If make solid culture medium, then add 2% agar powder (Biosharp company) again.Compound method:
(1) dissolving 10g yeast soaks powder, and the 20g tryptone as is made the dull and stereotyped 20g of adding agar powder in 900ml water.
(2) high pressure is 121 ℃, 20min.
(3) add 100ml 10% glucose (glucose sugar juice sterilization back adds).
Annotate: glucose, yeast soaks powder, and chemical reaction at high temperature may take place after mixing in tryptone solution, causes medium component to change, so mix after will sterilizing respectively again.
2, the recovery of bacterial strain is cultivated
(1) draw 0.3-0.5ml appropriate liquid medium with aseptic straw, splash in the ampoul tube, light shaking makes the dissolving of freeze-drying thalline be suspension.
(2) draw thallus suspension liquid, transplant on suitable agar slant or plating medium, under the temperature of suggestion, cultivate then.Advise an ampoul tube bacterial classification 2 to 3 inclined-planes of only transferring.All bacterium powder must disposable using up.
Points for attention:
(1) before the actication of culture, ampoul tube is kept under 6-10 ℃ the environment.
(2) after some bacterial classification was preserved through freeze drying, period of delay was longer, needed double successive transfer culture ability normal growth.
3, the preparation of fermented liquid
Prepare the shaking table cultivation and use fermentation medium, with the preparation of 250ml conical flask, every bottle of specification 200ml medium, 121 ℃ of sterilizations of high pressure 20min.With the oese of the bacterium of going out picking 2-3 ring thalline on all right flat board of growth or the slant medium, be inoculated in the fermentation medium, concussion is evenly.The conical flask that has connect kind is put into the constant temperature shaking table, and 220rpm cultivated 3 days for 30 ℃, and it is subsequent use to take out 4 ℃ of refrigerations.
4, oranges and tangerines trypetid class is lured the preparation of bait formulation
(1) the hydrolysed ferment liquid of the unusual Hansenula yeast bacterium of preparation: hydrolysising condition is a pH value 6.0,50 ℃ of hydrolysis temperatures, hydrolysis time 18h, the enzyme concentration 2.0mg/mL of papain.
(2) the auxiliary bait formulation of preparation: get multivalence peptone, brown sugar, ammonium acetate, methyl p-hydroxybenzoate, add water to the full dose of prescription after, fully stirring and dissolving mixes.
(3) be matrix with unusual Hansenula yeast bacterium hydrolysed ferment liquid, add auxiliary bait formulation, mix.
Embodiment 2
A kind of oranges and tangerines trypetid class is lured bait formulation, is made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and the volume ratio of said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:0.5.
Said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 20%;
Brown sugar 8%;
Vinegar 10%;
Sodium Benzoate 0.1%;
Water surplus.
The preparation method is with embodiment 1.
Embodiment 3
A kind of oranges and tangerines trypetid class is lured bait formulation, is made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and the volume ratio of said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:2.
Said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 5%;
Brown sugar 20%;
Vinegar 20%;
Sodium Benzoate 0.3%;
Water surplus.
The preparation method is with embodiment 1.
Embodiment 4 oranges and tangerines trypetid classes lure bait formulation that the field attractant effect of citrus fruit fly is measured
1, test is divided into groups:
(1) unusual Hansenula yeast bacterium hydrolysed ferment liquid
(2) albumen bait formulation (the auxiliary bait formulation among the embodiment 1)
(3) the oranges and tangerines trypetid class of embodiment 1,2,3 is lured bait formulation (unusual Hansenula yeast bacterium hydrolysed ferment liquid+albumen bait formulation)
(4) unusual Hansenula yeast bacterium hydrolysed ferment liquid+sugar-vinegar liquid (volume ratio 1:1, the mass ratio of sugar, vinegar, water is 1:1:8 in the sugar-vinegar liquid)
(5) fruit auspicious special fruit fly attractant dilution (purchase in Hubei Grea Biotech Co., Ltd., dilution ratio is 1:2)
(6) sugar-vinegar liquid (mass ratio of sugar, vinegar, water is 1:1:8).
2, test method: get respectively and respectively handle 50ml and put into yellow trapping jar, hang in the shade liftoff 1.5m place, mandarin tree tree crown middle part with iron wire, each handles 3 repetitions.The even at random 20m at interval of different disposal is suspended in the experiment orangery.Observed 3 days the citrus fruit fly male and female number that record is lured continuously.Do contrast with sugar-vinegar liquid, calculate the lure coefficient of each bait formulation citrus fruit fly according to formula.
Lure the borer population (head) that the borer population (head) that coefficient=bait formulation to be measured lures/the contrast sugar-vinegar liquid lures
3, result of the test
Table 1: the different disposal bait formulation is to the citrus fruit fly attractant effect
Annotate: numeral is " mean+SD " in the table, and each is listed as and is illustrated in P < significant difference on 0.05 the level between different lowercases.
The bait formulation of different formulations is to the attractant effect significant difference (table 1) of citrus fruit fly.What attractant effect was best is unusual Hansenula yeast bacterium hydrolysed ferment liquid+albumen bait formulation prescription, is 3.52 times of control group sugar-vinegar liquid, and the male and female worm is all had attractant effect preferably.Simultaneously, unusual Hansenula yeast bacterium hydrolysed ferment liquid+sugar-vinegar liquid is better than the attractant effect of separately unusual Hansenula yeast fermented liquid, and significant difference explains that sugar-vinegar liquid has synergistic effect.Albumen bait formulation, unusual Hansenula yeast bacterium hydrolysed ferment liquid+sugar-vinegar liquid and unusual Hansenula yeast bacterium hydrolysed ferment liquid+all the attractant effect than fruit Rui Te is good for the albumen bait formulation, significant difference.
Embodiment 5 oranges and tangerines trypetid classes lure bait formulation that the field attractant effect of dacus dorsalis is measured
1, test is divided into groups:
(1) unusual Hansenula yeast bacterium hydrolysed ferment liquid
(2) unusual Hansenula yeast bacterium hydrolysed ferment liquid+sugar-vinegar liquid (volume ratio 1:1, the mass ratio of sugar, vinegar, water is 1:1:8 in the sugar-vinegar liquid)
(3) unusual Hansenula yeast bacterium hydrolysed ferment liquid+methyl eugenol (methyl eugenol volume ratio 1%)
(4) the oranges and tangerines trypetid class of embodiment 1,2,3 is lured bait formulation (unusual Hansenula yeast bacterium hydrolysed ferment liquid+albumen bait formulation)
(5) albumen bait formulation (the auxiliary bait formulation among the embodiment 1)
(6) methyl eugenol paraffin mixed liquor (methyl eugenol volume fraction 1%)
(7) sugar-vinegar liquid (mass ratio of sugar, vinegar, water is 1:1:8).
2, test method: measure the lure of 5ml respectively with pipettor, process with absorbent cotton absorption and lure core.Trapper is purchased in Ningbo NewCon Co., Ltd..Trapper is hung on tangerine tree crown middle part shady spot with iron wire, and each handles 3 repetitions, and different disposal is suspension at interval evenly.Per 2 days record experimental datas, the male and female citrus fruit fly quantity that statistics is lured, and change lure, as contrast, calculate the lure coefficient of each bait formulation with sugar-vinegar liquid to citrus fruit fly according to formula.
Lure the borer population (head) that the borer population (head) that coefficient=bait formulation to be measured lures/the contrast sugar-vinegar liquid lures
3, result of the test
Table 2: the different disposal bait formulation is to the dacus dorsalis attractant effect
Annotate: numeral is " mean+SD " in the table, and each is listed as and is illustrated in P < significant difference on 0.05 the level between different lowercases.
The bait formulation of different formulations is to citrus fruit fly attractant effect significant difference.Oranges and tangerines trypetid class of the present invention is lured bait formulation attractant effect 2.7 times for the contrast sugar-vinegar liquid, also is superior to albumen bait formulation prescription simultaneously.Though unusual Hansenula yeast bacterium hydrolysed ferment liquid+methyl eugenol lures maximum borer populations; But the borer population there was no significant difference of luring bait formulation to lure with this experiment oranges and tangerines trypetid class; And it is bad for female attractant effect; Independent methyl eugenol paraffin mixed liquor can only lure male worm, takes all factors into consideration oranges and tangerines trypetid class and lures the attractant effect of bait formulation best.And there is significant difference in independent unusual Hansenula yeast bacterium hydrolysed ferment liquid with the attractant effect that has added the unusual Hansenula yeast bacterium hydrolysed ferment liquid of sugar-vinegar liquid, explains that sugar-vinegar liquid has synergistic effect to it.
Claims (5)
1. unusual Hansenula yeast bacterium (
Hansenula anomala) lure the application in the bait formulation in preparation oranges and tangerines trypetid class.
2. an oranges and tangerines trypetid class is lured bait formulation, it is characterized in that being made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 5-20%;
Brown sugar 8-20%;
Vinegar 10-20% or ammonium acetate 1.2-3%;
Preservative 0.1-0.3%;
Water surplus,
Wherein: preservative is Sodium Benzoate or methyl p-hydroxybenzoate,
The volume ratio of said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:0.5-2.
3. oranges and tangerines trypetid class as claimed in claim 2 is lured bait formulation, and the volume ratio that it is characterized in that said unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation is 1:1.
4. lure bait formulation like claim 2 or 3 described oranges and tangerines trypetid classes, it is characterized in that said auxiliary bait formulation is made up of the composition of following mass percent:
Multivalence peptone 15%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Water surplus.
5. an oranges and tangerines trypetid class is lured bait formulation, it is characterized in that being made up of unusual Hansenula yeast bacterium hydrolysed ferment liquid and auxiliary bait formulation, and its volume ratio is 1:1, and said auxiliary bait formulation is a sugar-vinegar liquid, and the mass ratio of sugar, vinegar, water is 1:1:8 in the said sugar-vinegar liquid.
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Cited By (8)
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| CN103740603A (en) * | 2014-01-26 | 2014-04-23 | 北京绿环国际科技有限公司 | Abnormal pichia anomala and application thereof |
| CN104542720A (en) * | 2014-12-19 | 2015-04-29 | 邓仁双 | Method for biologically controlling Bactrocera minax (Enderlein) |
| CN104892090A (en) * | 2015-04-30 | 2015-09-09 | 河北双星种业有限公司 | Multifunctional insecticidal and antibacterial nutrient solution and use method thereof |
| WO2017107872A1 (en) * | 2015-12-21 | 2017-06-29 | 云南省微生物发酵工程研究中心有限公司 | Microbial attractant, and use |
| CN107821392A (en) * | 2017-10-11 | 2018-03-23 | 华中农业大学 | A kind of long-acting tablets for having high epigamic to citrus trypetid and preparation method thereof |
| CN111449088A (en) * | 2019-04-22 | 2020-07-28 | 泰山医学院 | Novel lucilia sericata specific long-acting bacterial attractant and preparation method thereof |
| CN112602706A (en) * | 2021-01-09 | 2021-04-06 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
| CN115444007A (en) * | 2022-09-14 | 2022-12-09 | 漳州市美创生物科技有限公司 | Bactrocera dorsalis attractant and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103740603A (en) * | 2014-01-26 | 2014-04-23 | 北京绿环国际科技有限公司 | Abnormal pichia anomala and application thereof |
| CN103740603B (en) * | 2014-01-26 | 2015-08-05 | 北京绿环国际科技有限公司 | A kind of Abnormal pichia anomala and application thereof |
| CN104542720A (en) * | 2014-12-19 | 2015-04-29 | 邓仁双 | Method for biologically controlling Bactrocera minax (Enderlein) |
| CN104892090A (en) * | 2015-04-30 | 2015-09-09 | 河北双星种业有限公司 | Multifunctional insecticidal and antibacterial nutrient solution and use method thereof |
| WO2017107872A1 (en) * | 2015-12-21 | 2017-06-29 | 云南省微生物发酵工程研究中心有限公司 | Microbial attractant, and use |
| CN107821392A (en) * | 2017-10-11 | 2018-03-23 | 华中农业大学 | A kind of long-acting tablets for having high epigamic to citrus trypetid and preparation method thereof |
| CN107821392B (en) * | 2017-10-11 | 2020-09-29 | 华中农业大学 | Long-acting tablet with high attractability to citrus fruit flies and preparation method thereof |
| CN111449088A (en) * | 2019-04-22 | 2020-07-28 | 泰山医学院 | Novel lucilia sericata specific long-acting bacterial attractant and preparation method thereof |
| CN112602706A (en) * | 2021-01-09 | 2021-04-06 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
| CN112602706B (en) * | 2021-01-09 | 2022-02-01 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
| CN115444007A (en) * | 2022-09-14 | 2022-12-09 | 漳州市美创生物科技有限公司 | Bactrocera dorsalis attractant and preparation method and application thereof |
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