CN101697739A - bactrocera dorsalis hendel attractant - Google Patents
bactrocera dorsalis hendel attractant Download PDFInfo
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- CN101697739A CN101697739A CN200910193374A CN200910193374A CN101697739A CN 101697739 A CN101697739 A CN 101697739A CN 200910193374 A CN200910193374 A CN 200910193374A CN 200910193374 A CN200910193374 A CN 200910193374A CN 101697739 A CN101697739 A CN 101697739A
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- attractant
- hydrolysate
- bactrocera dorsalis
- dorsalis hendel
- citrus fruit
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- 239000005667 attractant Substances 0.000 title claims abstract description 43
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 43
- 241001124181 Bactrocera dorsalis Species 0.000 title abstract 5
- 239000000413 hydrolysate Substances 0.000 claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 12
- 230000007062 hydrolysis Effects 0.000 claims description 19
- 238000006460 hydrolysis reaction Methods 0.000 claims description 19
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 13
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 13
- 235000019710 soybean protein Nutrition 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 108091005508 Acid proteases Proteins 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 235000013399 edible fruits Nutrition 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 229940001941 soy protein Drugs 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 241000255588 Tephritidae Species 0.000 description 26
- 235000020971 citrus fruits Nutrition 0.000 description 25
- 230000000694 effects Effects 0.000 description 13
- 239000000758 substrate Substances 0.000 description 13
- 108010009736 Protein Hydrolysates Proteins 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- 239000002131 composite material Substances 0.000 description 9
- JFLRKDZMHNBDQS-UCQUSYKYSA-N CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C(=C[C@H]3[C@@H]2CC(=O)O1)C)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C.CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C=C[C@H]3C2CC(=O)O1)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C Chemical compound CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C(=C[C@H]3[C@@H]2CC(=O)O1)C)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C.CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C=C[C@H]3C2CC(=O)O1)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C JFLRKDZMHNBDQS-UCQUSYKYSA-N 0.000 description 8
- 239000005930 Spinosad Substances 0.000 description 8
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- 230000002421 anti-septic effect Effects 0.000 description 7
- 108090000604 Hydrolases Proteins 0.000 description 5
- 102000004157 Hydrolases Human genes 0.000 description 5
- ZYEMGPIYFIJGTP-UHFFFAOYSA-N O-methyleugenol Chemical compound COC1=CC=C(CC=C)C=C1OC ZYEMGPIYFIJGTP-UHFFFAOYSA-N 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
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- 229940116837 methyleugenol Drugs 0.000 description 2
- PRHTXAOWJQTLBO-UHFFFAOYSA-N methyleugenol Natural products COC1=CC=C(C(C)=C)C=C1OC PRHTXAOWJQTLBO-UHFFFAOYSA-N 0.000 description 2
- 241000256844 Apis mellifera Species 0.000 description 1
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- 241000317929 Chalcididae Species 0.000 description 1
- GUTLYIVDDKVIGB-OUBTZVSYSA-N Cobalt-60 Chemical compound [60Co] GUTLYIVDDKVIGB-OUBTZVSYSA-N 0.000 description 1
- 241000122995 Cynipoidea Species 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
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- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- NNWHUJCUHAELCL-SNAWJCMRSA-N Methyl isoeugenol Natural products COC1=CC=C(\C=C\C)C=C1OC NNWHUJCUHAELCL-SNAWJCMRSA-N 0.000 description 1
- 241000508269 Psidium Species 0.000 description 1
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- YKPQYKBIAFGWBT-KAMYIIQDSA-N [(Z)-18-hydroxyoctadec-9-en-7-yl] acetate Chemical compound CCCCCCC(C\C=C/CCCCCCCCO)OC(C)=O YKPQYKBIAFGWBT-KAMYIIQDSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
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- 239000003905 agrochemical Substances 0.000 description 1
- LPBNLHQGBCOYOT-UHFFFAOYSA-N anisole;butan-2-one Chemical compound CCC(C)=O.COC1=CC=CC=C1 LPBNLHQGBCOYOT-UHFFFAOYSA-N 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- NNWHUJCUHAELCL-UHFFFAOYSA-N cis-Methyl isoeugenol Natural products COC1=CC=C(C=CC)C=C1OC NNWHUJCUHAELCL-UHFFFAOYSA-N 0.000 description 1
- BJIOGJUNALELMI-ARJAWSKDSA-N cis-isoeugenol Chemical compound COC1=CC(\C=C/C)=CC=C1O BJIOGJUNALELMI-ARJAWSKDSA-N 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactrocera dorsalis hendel attractant, wherein the prescription of the attractant is a hydrolysate obtained by enzyme hydrolyzing soy protein and saccharomyces verevisiae protein according a certain ratio under some condition. The attractant not only is good for the environmental protection and the fruit safety, but also can prevent from environment pollution, and can not threaten human health and natural enemy of the bactrocera dorsalis hendel. The attractant has simple preparation method and low preparation cost, and can lead the hydrolysate to stably keep higher attractive force. The attractant has good attractive function to the female and fame bactrocera dorsalis hendel, thereby being capable of controlling the amount of species group of the bactrocera dorsalis hendel, reducing damage rate, and having good popularization meaning.
Description
Technical field
The invention belongs to field of pest control, be specifically related to a kind of attractant for juxiaoshi fly.
Background technology
Citrus fruit fly (Bactracera dorasalis) is worldwide quarantine pest insect, it remembers that scope is wide, fertility is strong, can cause harm 46 sections more than 250 kind of fruits and vegetables, vegetables and flowers such as guava, mango, be a kind of crushing insect, cause serious economic loss at present a plurality of national fruits and vegetables industries, flower industry.
Following several method is mainly adopted in detection and the control of citrus fruit fly both at home and abroad at present:
1, chemosynthesis attractant: found methyl eugenol at present, lure ketone, number of chemical material such as methoxybenzene butanone all has certain attracting action to citrus fruit fly that what wherein be most widely used is methyl eugenol and isoeugenol.
2, the utilization of natural enemy: found the larva parasitic wasp of multiple citrus fruit fly at present, trypetid cocoon honeybee, encyrtid, gall wasps have been arranged, gold chalcid fly etc., abroad some releasing research had been carried out in some areas, and certain effect is arranged.
3, the utilization of sterile technology: by the pupa irradiation cobalt 60 with citrus fruit fly, the infertile male imago that will sprout wings then is discharged in the orchard and goes, and makes female worm infertile.
4, food is lured control: the food attractant that is adopted all over the world at present comprises four big classes such as protein-based, yeast class, molasses class and natural plants.The sexual maturity of citrus fruit fly adult and lay eggs and water soluble protein must be arranged as nutrition, and can lure female worm and male worm simultaneously by the bait of its preparation, be comparatively ideal attractant.
In the said method, the method that relies on chemical control control citrus fruit fly is the most frequently used a kind of, but use agricultural chemicals to be unfavorable for environmental protection and the edible safety of fruit in a large number, ecotope is caused severe contamination and threatens human health, and applying pesticides also causes the citrus fruit fly pesticide resistance to rise continually, should not advocate.Gyplure only has attracting action to the citrus fruit fly male worm, and effect is limited aspect this population of control.And food is lured, and especially the protide inducing substance can be lured the female male worm of citrus fruit fly, thereby reaches its population quantity of direct control, reduce the effect of hazard ratio, and protein hydrolysate is also very safe to environment and citrus fruit fly natural enemy.
Bishopp reported first in 1916 utilize protein hydrolysate to lure the trypetid class pest, Shorey in 1997 and John report utilize protein hydrolysate to lure citrus fruit fly (Shorey HH, John JM.Chemicalcontrol of insect behavior theory and applicatior[M] .New York Wiley J, Ins S1997,327-344.), and China has also adopted protein hydrolysate class material to prevent and treat citrus fruit fly in the 1950's.After this get more and more and report about research in this respect.Sterier (1952) once reported and found that sugar and protolysate, enzymatic yeast hydrolysate, soya bean hydrolysate all had strong attracting action to citrus fruit fly, and to the attracting ability of female worm be better than male worm (Stenier LF.Mehyleugenol as an attractant for theoriental fruitfly[J] .J.Econ Entomol, 1952,45 (2): 241-248).Yet present its allure of existing protein hydrolysate is strong inadequately, and lasting effect is relatively poor, waits further screening and research.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of the female worm of citrus fruit fly is also had attracting action, and the strong attractant for juxiaoshi fly of allure.
Above-mentioned purpose of the present invention is achieved by following scheme:
A kind of attractant for juxiaoshi fly, this attractant is the food attractant that belongs to protein hydrolysate, in order to obtain the higher rate of luring, especially the female worm of citrus fruit fly also had strong attracting action, the inventor is after studying numerous protein hydrolysate, discovery with soybean protein and brewer's yeast albumen composite hydrolysis after, the hydrolysate that obtains can reach above-mentioned purpose as attractant.
Attractant for juxiaoshi fly of the present invention can be the hydrolysate that soybean protein and brewer's yeast albumen is carried out obtain after basic hydrolysis, acidic hydrolysis or the enzymatic hydrolysis, the hydrolysate that the preferred enzyme hydrolysis obtains, used enzyme can be for any, as acid protease etc.
In order to make the gained attractant have better attractant effect, the present invention optimizes hydrolysis substrate prescription and some hydrolysising conditions:
1. hydrolysis substrate soybean protein and brewer's yeast albumen, its mixed proportion is 1: 1,1: 3 or 1: 9;
2. the liquid-solid ratio of hydrolase is 10: 1 (V/W), and the adding total amount of enzyme is 5000 (U/g);
3. soybean protein and the brewer's yeast albumen reaction that under the effect of hydrolase, is hydrolyzed, its reaction temperature is 50 ℃, the reaction time is 2 hours.
4. hydrolysate attractant effect under acid pH is better, therefore can be not the whole pH of hydrolysate not be regulated, and can be 3.4 or 4 with the whole pH regulator of hydrolysate also;
5. add 0.5～2.0% preservative and 0.01～0.1% pleocidin (Spinosad) in hydrolysate again, aforementioned percentage is the percentage that accounts for the hydrolysate gross mass.
Said hydrolyzed substrate prescription and hydrolysising condition can also further be optimized, and its scheme is:
1. hydrolysis substrate soybean protein and brewer's yeast albumen, its mixed proportion is 1: 3;
2. the liquid-solid ratio of hydrolase is 10: 1, and the adding total amount of enzyme is 5000U/g;
3. soybean protein and the brewer's yeast albumen reaction that under the effect of hydrolase, is hydrolyzed, its reaction temperature is 50 ℃, the reaction time is 2 hours;
4. the whole pH of hydrolysate is 4;
5. add 0.5% preservative and 0.01% pleocidin in the hydrolysate.
Compared with prior art, the present invention has following beneficial effect:
1. attractant of the present invention adopts the composite hydrolysis protein prescription, not only is beneficial to the edible safety of environmental protection and fruit, and ecotope can not pollute, and also can not threaten the safety of human health and citrus fruit fly natural enemy;
2. attractant of the present invention adopts enzymatic hydrolysis to prepare hydrolysate, by enzyme kinetics and orthogonal experiment research, just can determine the optimal selection scheme of the reaction conditions such as concentration, hydrolysis time, hydrolysising reacting temperature and pH of enzyme and hydrolysis substrate, not only the preparation method is simple, production cost is low, and can guarantee that hydrolysate can stably keep higher allure;
3. attractant of the present invention has also added pleocidin in its hydrolysate, pleocidin is a kind of macrolides compound, has the safety of biopesticide and the quick-acting of chemical pesticide concurrently;
Therefore 4. attractant of the present invention all has attracting action preferably to the female male worm of citrus fruit fly, can realize being with the promotion of good sense the purpose controlling the citrus fruit fly population quantity, reduce hazard ratio.
Embodiment
Conditions such as compound protein hydrolysis substrate and product are carried out orthogonal experiment, select optimal scheme, each factor that participates in orthogonal experiment is as follows:
(1) composite hydrolysis substrate: the proportion of composing of soybean protein and brewer's yeast albumen is 1: 1,1: 3,1: 9;
(2) antiseptic content: 0.5,1.0,2.0;
(3) Spinosad content: 0.01,0.05,0.1;
(4) the whole pH of hydrolysate: adjusting, 3.5,4;
Hydrolase is selected acid protease, and the liquid-solid ratio of enzyme is 10: 1, and the adding total amount of enzyme is 5000 (U/g), and the hydrolysis temperature during hydrolysis is 50 ℃, is hydrolyzed to 2 hours.
Four factors that table 1 is depicted as that orthogonal experiment need study and numerical value thereof.
Table 1 orthogonal experiment factor level table
Table 2～table 5 is depicted as orthogonal experiment plan and takes into account attractant effect to the female worm of citrus fruit fly 2～3 ages in days, female male worm, and to the attractant effect of the female worm of spawning period citrus fruit fly, female male worm.
Table 2 orthogonal experiment plan is taken into account the female worm attractant effect of citrus fruit fly 2～3 ages in days
| Tested number | The composite hydrolysis substrate | Antiseptic content | Spinosad content | The pH value | Lure rate (%) |
| ??1 | ??1 | ??1 | ??1 | ??1 | ??38 |
| ??2 | ??1 | ??2 | ??2 | ??2 | ??46 |
| ??3 | ??1 | ??3 | ??3 | ??3 | ??34 |
| ??4 | ??2 | ??1 | ??2 | ??3 | ??46 |
| ??5 | ??2 | ??2 | ??3 | ??1 | ??42 |
| ??6 | ??2 | ??3 | ??1 | ??2 | ??52 |
| ??7 | ??3 | ??1 | ??3 | ??2 | ??40 |
| ??8 | ??3 | ??2 | ??1 | ??3 | ??42 |
| ??9 | ??3 | ??3 | ??2 | ??1 | ??30 |
| ??K1 | ??118 | ??130 | ??132 | ??110 | |
| ??K2 | ??140 | ??124 | ??122 | ??138 |
| Tested number | The composite hydrolysis substrate | Antiseptic content | Spinosad content | The pH value | Lure rate (%) |
| ??K3 | ??112 | ??116 | ??116 | ??122 | |
| ??k1 | ??39.33 | ??43.33 | ??44.00 | ??36.66 | |
| ??k2 | ??46.67 | ??40.33 | ??40.66 | ??46.00 | |
| ??k3 | ??37.33 | ??38.66667 | ??38.67 | ??40.67 | |
| ??R | ??9.33 | ??4.67 | ??5.33 | ??9.33 |
Table 3 orthogonal experiment plan is taken into account the female male worm attractant effect of citrus fruit fly 2～3 ages in days
| Tested number | The composite hydrolysis substrate | Antiseptic content | Spinosad content | The pH value | Lure rate (%) |
| ??1 | ??1 | ??1 | ??1 | ??1 | ??32 |
| ??2 | ??1 | ??2 | ??2 | ??2 | ??40 |
| ??3 | ??1 | ??3 | ??3 | ??3 | ??39 |
| ??4 | ??2 | ??1 | ??2 | ??3 | ??49 |
| ??5 | ??2 | ??2 | ??3 | ??1 | ??37 |
| ??6 | ??2 | ??3 | ??1 | ??2 | ??45 |
| ??7 | ??3 | ??1 | ??3 | ??2 | ??43 |
| ??8 | ??3 | ??2 | ??1 | ??3 | ??41 |
| ??9 | ??3 | ??3 | ??2 | ??1 | ??28 |
| ??K1 | ??111 | ??124 | ??119 | ??97 | |
| ??K2 | ??131 | ??118 | ??117 | ??129 | |
| ??K3 | ??112 | ??112 | ??118 | ??128 | |
| ??k1 | ??37.00 | ??41.33 | ??39.67 | ??32.33 | |
| ??k2 | ??43.67 | ??39.33 | ??39.00 | ??43.00 | |
| ??k3 | ??37.33 | ??37.33 | ??39.33 | ??42.67 |
| Tested number | The composite hydrolysis substrate | Antiseptic content | Spinosad content | The pH value | Lure rate (%) |
| ??R | ??6.67 | ??4.00 | ??0.67 | ??10.67 |
Annotate: K1, K2 and K3 are each factor 1,2,3 level sums as a result; R is the extreme difference with k1, k2 and k3 under the factor.
Table 4 orthogonal experiment plan is taken into account the female worm attractant effect of spawning period citrus fruit fly
| Tested number | The composite hydrolysis substrate | Antiseptic content | Spinosad content | The pH value | Lure rate (%) |
| ??1 | ??1 | ??1 | ??1 | ??1 | ??54 |
| ??2 | ??1 | ??2 | ??2 | ??2 | ??50 |
| ??3 | ??1 | ??3 | ??3 | ??3 | ??46 |
| ??4 | ??2 | ??1 | ??2 | ??3 | ??74 |
| ??5 | ??2 | ??2 | ??3 | ??1 | ??64 |
| ??6 | ??2 | ??3 | ??1 | ??2 | ??80 |
| ??7 | ??3 | ??1 | ??3 | ??2 | ??44 |
| ??8 | ??3 | ??2 | ??1 | ??3 | ??66 |
| ??9 | ??3 | ??3 | ??2 | ??1 | ??54 |
| ??K1 | ??150 | ??172 | ??200 | ??172 | |
| ??K2 | ??218 | ??180 | ??178 | ??174 | |
| ??K3 | ??164 | ??180 | ??154 | ??186 | |
| ??k1 | ??50.00 | ??57.33 | ??66.67 | ??57.33 | |
| ??k2 | ??72.67 | ??60.00 | ??59.33 | ??58.00 | |
| ??k3 | ??54.67 | ??60.00 | ??51.33 | ??62.00 | |
| ??R | ??22.67 | ??2.67 | ??15.33 | ??4.67 |
Annotate: K1, K2 and K3 are each factor 1,2,3 level sums as a result; R is the extreme difference with k1, k2 and k3 under the factor.
Table 5 orthogonal experiment plan is taken into account the female male worm attractant effect of spawning period citrus fruit fly
| Tested number | The composite hydrolysis substrate | Antiseptic content | Spinosad content | The pH value | Lure rate (%) |
| ??1 | ??1 | ??1 | ??1 | ??1 | ??59 |
| ??2 | ??1 | ??2 | ??2 | ??2 | ??38 |
| ??3 | ??1 | ??3 | ??3 | ??3 | ??40 |
| ??4 | ??2 | ??1 | ??2 | ??3 | ??69 |
| ??5 | ??2 | ??2 | ??3 | ??1 | ??68 |
| ??6 | ??2 | ??3 | ??1 | ??2 | ??81 |
| ??7 | ??3 | ??1 | ??3 | ??2 | ??44 |
| ??8 | ??3 | ??2 | ??1 | ??3 | ??64 |
| ??9 | ??3 | ??3 | ??2 | ??1 | ??55 |
| ??K1 | ??137 | ??172 | ??204 | ??182 | |
| ??K2 | ??218 | ??170 | ??162 | ??163 | |
| ??K3 | ??163 | ??176 | ??152 | ??173 | |
| ??k1 | ??45.67 | ??57.33 | ??68.00 | ??60.67 | |
| ??k2 | ??72.67 | ??56.67 | ??54.00 | ??54.33 | |
| ??k3 | ??54.33 | ??58.67 | ??50.67 | ??57.67 | |
| ??R | ??27.00 | ??2.00 | ??17.33 | ??6.33 |
Annotate: K1, K2 and K3 are each factor 1,2,3 level sums as a result; R is the extreme difference with k1, k2 and k3 under the factor.
Claims (10)
1. attractant for juxiaoshi fly, this attractant is the hydrolysate of soybean protein and brewer's yeast albumen.
2. according to the described attractant of claim 1, it is characterized in that described attractant is the enzymic hydrolysates of soybean protein and brewer's yeast albumen.
3. according to the described attractant of claim 2, it is characterized in that described enzyme is an acid protease.
4. according to the described attractant of claim 2, the liquid-solid ratio that it is characterized in that described enzyme is 10: 1, and the adding total amount of enzyme is 5000U/g.
5. according to the described attractant of claim 1, the ratio that it is characterized in that described soybean protein and brewer's yeast albumen is 1: 1,1: 3 or 1: 9.
6. according to the described attractant of claim 5, the ratio that it is characterized in that described soybean protein and brewer's yeast albumen is 1: 3.
7. according to the described attractant of claim 1, it is characterized in that also being added with in the described hydrolysate that to account for the hydrolysate gross mass be 0.5～2.0% preservative, account for the hydrolysate gross mass and be 0.01～0.1% pleocidin.
8. according to the described attractant of claim 7, the addition that it is characterized in that described preservative is 0.5%, and the addition of pleocidin is 0.01%.
9. according to the described attractant of claim 1, the reaction temperature that it is characterized in that described hydrolysis is 50 ℃, and the reaction time is 2 hours.
10. according to the described attractant of claim 1, the pH that it is characterized in that described hydrolysate is 3.5 or 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910193374 CN101697739B (en) | 2009-10-27 | 2009-10-27 | Bactrocera dorsalis hendel attractant |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910193374 CN101697739B (en) | 2009-10-27 | 2009-10-27 | Bactrocera dorsalis hendel attractant |
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| Publication Number | Publication Date |
|---|---|
| CN101697739A true CN101697739A (en) | 2010-04-28 |
| CN101697739B CN101697739B (en) | 2013-05-01 |
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| CN 200910193374 Expired - Fee Related CN101697739B (en) | 2009-10-27 | 2009-10-27 | Bactrocera dorsalis hendel attractant |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487944A (en) * | 2011-12-16 | 2012-06-13 | 东莞市盛唐化工有限公司 | Improved food attractant for citrus fruit fly |
| CN102499269A (en) * | 2011-10-11 | 2012-06-20 | 华南农业大学 | Composite attractant of bactrocera dorsalis hendel |
| CN102754665A (en) * | 2012-07-17 | 2012-10-31 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1810109A (en) * | 2005-12-27 | 2006-08-02 | 张颂声 | Pesticide for killing solenopsis invicta |
| CN101066096A (en) * | 2007-03-30 | 2007-11-07 | 广东省农业科学院果树研究所 | Artificial raising of orange fly larva |
| CN101189974A (en) * | 2007-11-30 | 2008-06-04 | 张颂声 | Safety environmental protection bait agent for red imported fire ant |
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2009
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499269A (en) * | 2011-10-11 | 2012-06-20 | 华南农业大学 | Composite attractant of bactrocera dorsalis hendel |
| CN102487944A (en) * | 2011-12-16 | 2012-06-13 | 东莞市盛唐化工有限公司 | Improved food attractant for citrus fruit fly |
| CN102487944B (en) * | 2011-12-16 | 2013-08-28 | 东莞市盛唐化工有限公司 | Improved food attractant for citrus fruit fly |
| CN102754665A (en) * | 2012-07-17 | 2012-10-31 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
| CN102754665B (en) * | 2012-07-17 | 2014-05-14 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
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| Publication number | Publication date |
|---|---|
| CN101697739B (en) | 2013-05-01 |
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