CN101697739B

CN101697739B - Bactrocera dorsalis hendel attractant

Info

Publication number: CN101697739B
Application number: CN 200910193374
Authority: CN
Inventors: 曾玲; 朱林娟; 许益镌; 陆永跃
Original assignee: South China Agricultural University
Current assignee: South China Agricultural University
Priority date: 2009-10-27
Filing date: 2009-10-27
Publication date: 2013-05-01
Anticipated expiration: 2029-10-27
Also published as: CN101697739A

Abstract

The invention discloses a bactrocera dorsalis hendel attractant, wherein the prescription of the attractant is a hydrolysate obtained by enzyme hydrolyzing soy protein and saccharomyces verevisiae protein according a certain ratio under some condition. The attractant not only is good for the environmental protection and the fruit safety, but also can prevent from environment pollution, and can not threaten human health and natural enemy of the bactrocera dorsalis hendel. The attractant has simple preparation method and low preparation cost, and can lead the hydrolysate to stably keep higher attractive force. The attractant has good attractive function to the female and fame bactrocera dorsalis hendel, thereby being capable of controlling the amount of species group of the bactrocera dorsalis hendel, reducing damage rate, and having good popularization meaning.

Description

A kind of attractant for juxiaoshi fly

Technical field

The invention belongs to field of pest control, be specifically related to a kind of attractant for juxiaoshi fly.

Background technology

Citrus fruit fly (Bactracera dorasalis) is worldwide quarantine pest insect, it remembers that scope is wide, fertility is strong, can cause harm more than 250 kind of fruits and vegetables of 46 sections, the vegetables and flowers such as guava, mango, be a kind of crushing insect, cause serious economic loss at present a plurality of national fruits and vegetables industries, flower industry.

Following several method is mainly adopted in detection and the control of citrus fruit fly both at home and abroad at present:

1, chemosynthesis attractant: found methyl eugenol at present, lure ketone, the number of chemical material such as methoxybenzene butanone all has certain attracting action to citrus fruit fly that what wherein be most widely used is methyl eugenol and isoeugenol.

2, the utilization of natural enemy: found the parasitized larvae honeybee of multiple citrus fruit fly at present, trypetid cocoon honeybee, encyrtid, gall wasps have been arranged, gold chalcid fly etc., abroad some releasing research had been carried out in some areas, and certain effect is arranged.

3, the utilization of sterile technology: by the pupa irradiation cobalt 60 with citrus fruit fly, the infertile male imago that then will sprout wings is discharged in the orchard and goes, and makes female worm infertile.

4, food is lured control: the food attractant that adopts all over the world at present comprises the four large classes such as protein-based, yeast class, molasses class and natural plants.The sexual maturity of citrus fruit fly adult and lay eggs and water soluble protein must be arranged as nutrition, and can lure simultaneously female worm and male worm by the bait of its preparation, be comparatively ideal attractant.

In the said method, the method that relies on chemical control control citrus fruit fly is the most frequently used a kind of, but use in a large number agricultural chemicals to be unfavorable for environmental protection and the edible safety of fruit, ecotope is caused severe contamination and threatens human health, and applying pesticides also causes the citrus fruit fly pesticide resistance to rise continually, should not advocate.Gyplure only has attracting action to the citrus fruit fly male worm, and effect is limited aspect this population of control.And food is lured, and especially the protide inducing substance can be lured the female male worm of citrus fruit fly, thereby reaches its population quantity of direct control, reduce the effect of hazard ratio, and protein hydrolysate is also very safe to environment and citrus fruit fly natural enemy.

Bishopp reported first in 1916 utilize protein hydrolysate to lure Fruit fly, Shorey in 1997 and John report utilize protein hydrolysate to lure citrus fruit fly (Shorey HH, John JM.Chemicalcontrol of insect behavior theory and applicatior[M] .New York Wiley J, Ins S1997,327-344.), and China has also adopted protein hydrolysate class material to prevent and treat citrus fruit fly in the 1950's.After this get more and more and report about research in this respect.Sterier (1952) once reported and found that sugar and protolysate, enzymatic yeast hydrolysate, soya bean hydrolysate had strong attracting action to citrus fruit fly, and to the attracting ability of female worm be better than male worm (Stenier LF.Mehyleugenol as an attractant for theoriental fruitfly[J] .J.Econ Entomol, 1952,45 (2): 241-248).Yet present its allure of existing protein hydrolysate is strong not, and lasting effect is relatively poor, waits further screening and research.

Summary of the invention

The object of the invention is to for the deficiencies in the prior art, provide a kind of the female worm of citrus fruit fly is also had attracting action, and the strong attractant for juxiaoshi fly of allure.

Above-mentioned purpose of the present invention is achieved by following scheme:

A kind of attractant for juxiaoshi fly, this attractant is the food attractant that belongs to protein hydrolysate, in order to obtain the higher rate of luring, especially the female worm of citrus fruit fly also had stronger attracting action, the inventor is after studying numerous protein hydrolysate, discovery with soybean protein and brewer's yeast albumen composite hydrolysis after, the hydrolysate that obtains can reach above-mentioned purpose as attractant.

Attractant for juxiaoshi fly of the present invention can be the hydrolysate that soybean protein and brewer's yeast albumen is carried out obtain after basic hydrolysis, acidic hydrolysis or the enzymatic hydrolysis, the hydrolysate that the preferred enzyme hydrolysis obtains, used enzyme can be for any, such as acid protease etc.

In order to make the gained attractant have better attractant effect, the present invention is optimized hydrolysis substrate prescription and some hydrolysising conditions:

1. hydrolysis substrate soybean protein and brewer's yeast albumen, its mixed proportion is 1: 1,1: 3 or 1: 9;

2. the liquid-solid ratio of hydrolase is 10: 1 (V/W), and the adding total amount of enzyme is 5000 (U/g);

3. soybean protein and the brewer's yeast albumen reaction that under the effect of hydrolase, is hydrolyzed, its reaction temperature is 50 ℃, the reaction time is 2 hours.

Therefore 4. hydrolysate attractant effect under acid pH is better, can be not the whole pH of hydrolysate not be regulated, and also the whole pH of hydrolysate can be adjusted to 3.4 or 4;

5. add 0.5～2.0% preservative and 0.01～0.1% pleocidin (Spinosad) in hydrolysate, aforementioned percentage is the percentage that accounts for the hydrolysate gross mass again.

Said hydrolyzed substrate prescription and hydrolysising condition can also further be optimized, and its scheme is:

1. hydrolysis substrate soybean protein and brewer's yeast albumen, its mixed proportion is 1: 3;

2. the liquid-solid ratio of hydrolase is 10: 1, and the adding total amount of enzyme is 5000U/g;

3. soybean protein and the brewer's yeast albumen reaction that under the effect of hydrolase, is hydrolyzed, its reaction temperature is 50 ℃, the reaction time is 2 hours;

4. the whole pH of hydrolysate is 4;

5. add 0.5% preservative and 0.01% pleocidin in the hydrolysate.

Compared with prior art, the present invention has following beneficial effect:

1. attractant of the present invention adopts the composite hydrolysis protein prescription, not only is beneficial to the edible safety of environmental protection and fruit, and ecotope can not pollute, and also can not threaten the safety of human health and citrus fruit fly natural enemy;

2. attractant of the present invention adopts enzymatic hydrolysis to prepare hydrolysate, by enzyme kinetics and orthogonal test research, just can determine the optimal selection scheme of the reaction conditions such as concentration, hydrolysis time, hydrolysising reacting temperature and pH of enzyme and hydrolysis substrate, not only the preparation method is simple, production cost is low, and can guarantee that hydrolysate can stably keep higher allure;

3. attractant of the present invention has also added pleocidin in its hydrolysate, and pleocidin is a kind of macrolides compound, has the safety of biopesticide and the quick-acting of chemical pesticide concurrently;

Therefore 4. attractant of the present invention all has preferably attracting action to the female male worm of citrus fruit fly, can realize being with the promotion of good sense the purpose controlling the citrus fruit fly population quantity, reduce hazard ratio.

Embodiment

The conditions such as compound protein hydrolysis substrate and product are carried out orthogonal experiment, select best scheme, each factor that participates in orthogonal experiment is as follows:

(1) composite hydrolysis substrate: the proportion of composing of soybean protein and brewer's yeast albumen is 1: 1,1: 3,1: 9;

(2) antiseptic content: 0.5,1.0,2.0;

(3) Spinosad content: 0.01,0.05,0.1;

(4) the whole pH of hydrolysate: adjusting, 3.5,4;

Hydrolase is selected acid protease, and the liquid-solid ratio of enzyme is 10: 1, and the adding total amount of enzyme is 5000 (U/g), and the hydrolysis temperature during hydrolysis is 50 ℃, is hydrolyzed to 2 hours.

Four factors that table 1 is depicted as that orthogonal experiment need to study and numerical value thereof.

Table 1 orthogonal experiment factor level table

Table 2～table 5 is depicted as orthogonal experiment plan and takes into account attractant effect to the female worm of citrus fruit fly 2～3 ages in days, female male worm, and to the attractant effect of the female worm of spawning period citrus fruit fly, female male worm.

Table 2 orthogonal experiment plan is taken into account the female worm attractant effect of citrus fruit fly 2～3 ages in days

Tested number	The composite hydrolysis substrate	Antiseptic content	Spinosad content	The pH value	Lure rate (%)
						1	1	1	1	1	38
2	1	2	2	2	46
						3	1	3	3	3	34
4	2	1	2	3	46
						5	2	2	3	1	42
6	2	3	1	2	52
						7	3	1	3	2	40
8	3	2	1	3	42
						9	3	3	2	1	30
K1	118	130	132	110
						K2	140	124	122	138
K3	112	116	116	122
						k1	39.33	43.33	44.00	36.66
k2	46.67	40.33	40.66	46.00
						k3	37.33	38.66667	38.67	40.67
R	9.33	4.67	5.33	9.33

Table 3 orthogonal experiment plan is taken into account the female male worm attractant effect of citrus fruit fly 2～3 ages in days

Tested number	The composite hydrolysis substrate	Antiseptic content	Spinosad content	The pH value	Lure rate (%)
						1	1	1	1	1	32
2	1	2	2	2	40
						3	1	3	3	3	39
4	2	1	2	3	49
						5	2	2	3	1	37
6	2	3	1	2	45
						7	3	1	3	2	43
8	3	2	1	3	41
						9	3	3	2	1	28
K1	111	124	119	97
						K2	131	118	117	129
K3	112	112	118	128
						k1	37.00	41.33	39.67	32.33
k2	43.67	39.33	39.00	43.00
						k3	37.33	37.33	39.33	42.67
R	6.67	4.00	0.67	10.67

Annotate: K1, K2 and K3 are as a result sum of each factor 1,2,3 levels; R is the extreme difference with k1, k2 and k3 under the factor.

Table 4 orthogonal experiment plan is taken into account the female worm attractant effect of spawning period citrus fruit fly

Tested number	The composite hydrolysis substrate	Antiseptic content	Spinosad content	The pH value	Lure rate (%)
						1	1	1	1	1	54
2	1	2	2	2	50
						3	1	3	3	3	46
4	2	1	2	3	74
						5	2	2	3	1	64
6	2	3	1	2	80
						7	3	1	3	2	44
8	3	2	1	3	66
						9	3	3	2	1	54
K1	150	172	200	172
						K2	218	180	178	174
K3	164	180	154	186
						k1	50.00	57.33	66.67	57.33
k2	72.67	60.00	59.33	58.00
						k3	54.67	60.00	51.33	62.00
R	22.67	2.67	15.33	4.67

Table 5 orthogonal experiment plan is taken into account the female male worm attractant effect of spawning period citrus fruit fly

Tested number	The composite hydrolysis substrate	Antiseptic content	Spinosad content	The pH value	Lure rate (%)
						1	1	1	1	1	59
2	1	2	2	2	38
						3	1	3	3	3	40
4	2	1	2	3	69
						5	2	2	3	1	68
6	2	3	1	2	81
						7	3	1	3	2	44
8	3	2	1	3	64
						9	3	3	2	1	55
K1	137	172	204	182
						K2	218	170	162	163
K3	163	176	152	173
						k1	45.67	57.33	68.00	60.67
k2	72.67	56.67	54.00	54.33
						k3	54.33	58.67	50.67	57.67
R	27.00	2.00	17.33	6.33

Claims

1. an attractant for juxiaoshi fly is characterized in that this attractant is the enzymic hydrolysates of soybean protein and brewer's yeast albumen;

Wherein, described enzyme is acid protease; The liquid-solid ratio of described enzyme is 10:1, and the adding total amount of enzyme is 5000U/g;

The ratio of described soybean protein and brewer's yeast albumen is 1:1,1:3 or 1:9;

The reaction temperature of described hydrolysis is 50 ℃, and the reaction time is 2 hours;

The pH of described hydrolysate is 3.5 or 4.

2. described attractant for juxiaoshi fly according to claim 1, the ratio that it is characterized in that described soybean protein and brewer's yeast albumen is 1:3.

3. described attractant for juxiaoshi fly according to claim 1 is characterized in that also being added with in the described hydrolysate that to account for the hydrolysate gross mass be 0.5～2.0% preservative, accounts for the hydrolysate gross mass and be 0.01～0.1% pleocidin.

4. described attractant for juxiaoshi fly according to claim 3, the addition that it is characterized in that described preservative is 0.5%, the addition of pleocidin is 0.01%.