CN102499269A - Composite attractant of bactrocera dorsalis hendel - Google Patents
Composite attractant of bactrocera dorsalis hendel Download PDFInfo
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- CN102499269A CN102499269A CN2011103061747A CN201110306174A CN102499269A CN 102499269 A CN102499269 A CN 102499269A CN 2011103061747 A CN2011103061747 A CN 2011103061747A CN 201110306174 A CN201110306174 A CN 201110306174A CN 102499269 A CN102499269 A CN 102499269A
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Abstract
The invention discloses a composite attractant of bactrocera dorsalis hendel, which comprises aqueous solution of a molasses, aqueous solution of a complex protein hydrolyzate and a fruit flavor. The mass ratio of the aqueous solution of the molasses to the aqueous solution of the complex protein hydrolyzate is 4:1; the complex protein hydrolyzate is the hydrolysate which is obtained by mixing soy protein and brewer's yeast protein at the weight ratio of 1:1-9, adding 5,000U/g of acidic protease, 0.5-5.0 parts by weight of preservative and 0.01-0.1 part by weight of spinosad, and hydrolyzing at 50 DEG C for 2hours. The formula of the composite attractant of the bactrocera dorsalis hendel is simple, and the raw materials are cheap and easy to obtain. The composite attractant has good effect of attracting the male and female adult bactrocera dorsalis hendel, and particularly, the composite attractant can attract more than 25% of bactrocera dorsalis hendel of 10-11 days old. The raw materials used for preparing the composite attractant do not contain any harmful chemical substances, have no toxic effects on people and animals and meet the environmental protection requirements.
Description
Technical field
The present invention relates to the plant protection field, be specifically related to the compound attractant of a kind of citrus fruit fly.
Background technology
Citrus fruit fly (
Bactrocera dorsalisHendel), being subordinate to diptera (Diptera), is the torrid zone and the main fruits and vegetables insect in subtropical zone, dangerous great.Its host range is very wide, and 250 various crop fruits of 46 sections such as main harm mango, oranges and tangerines, carambola, papaya papaw are main with the torrid zone and semi-tropical fruit-vegetable plant.Citrus fruit fly is annual the generation in China south, and generation overlap is serious, and have 2 hazard booms an every year in Guangdong; Since May the adult generating capacity increase gradually; 1 bigger peak of occurrence occurs to August, occur 1 peak November again, up to the just slightly decline of adult generating capacity in December.The citrus fruit fly adult life-span reaches 1 year, can survive in the open air 4 ~ 5 months, and harm is serious.
Mainly rely on the harm of this worm of chemical control control all the time, but on producing unreasonable continually applying pesticides, the pesticide resistance of citrus fruit fly constantly strengthens, and causes the citrus fruit fly pesticide resistance to rise, and also threatens food security and human health simultaneously.The gyplure attractant of methyl eugenol (as contain) trapping is only worked to the citrus fruit fly male worm, and is invalid to female worm, aspect this population of control effect limited, and the control cost is high.Searching is the difficult point of research to the material that the citrus fruit fly female adult worm has attracting action always.
All can use methyl eugenol about attractant for juxiaoshi fly in the prior art, as 200510048764.9,200410051506.1,200610037490.8 etc.The hydrolysate of patent of invention 200910193374.9 usefulness soybean proteins and brewer's yeast albumen is mixed with attractant for juxiaoshi fly, and the male and female worm of citrus fruit fly is all had attracting action preferably.Application for a patent for invention 200710029157.7 discloses a kind of citrus fruit fly food attractant, and fruit aroma material 1 ~ 10% is contained in the inside, solvent 10 ~ 50%, nutritional agents 40 ~ 80%, preservative 1 ~ 10%.Wherein nutritional agents is one or more in carbohydrate, protein hydrolysate class, the bacterium class material, and carbohydrate is granulated sugar, sugar nibs, brown granulated sugar, brown sugar, brown powdered sugar, honey, molasses or fructose, and the protein hydrolysate class is casein, gelatin or Saccharomyces cerevisiae.This attractant has extremely strong attractant effect to citrus fruit fly male and female adult, and so the female-male proportion of being lured meets citrus fruit fly even by natural population sex ratio.But search out the big difficulty of attractant existence that the citrus fruit fly female insect has strong attracting action; This patent emphasis is to this problem, and the optimal proportion that obtains molasses, protein hydrolysate and fruit essence in the compound attractant is to reach the best attractant effect to female worm.
Summary of the invention
The objective of the invention is the problems referred to above, a kind of new compound attractant of citrus fruit fly that female worm and male worm is all had attractant effect is provided to attractant for juxiaoshi fly in the prior art.
The present invention realizes above-mentioned purpose through following technical scheme:
The compound attractant of a kind of citrus fruit fly is made up of molasses water solution, composite hydrolysis protein solution and fruit essence, and the mass ratio of said molasses water solution and composite hydrolysis protein solution is 4:1.
Said composite hydrolysis albumen is soybean protein to be mixed (preferred 1:1,1:3, three ratios of 1:9 by weight 1:1 ~ 9 with brewer's yeast albumen; Wherein 1:3 is best); Add acid protease 5000U/g; The product that hydrolysis obtained in 2 hours under 50 ℃ of conditions wherein also adds preservative 0.5 ~ 5.0 weight portion, pleocidin Spinosad 0.01 ~ 0.1 weight portion.
Above-mentioned composite hydrolysis albumen belongs to the present invention's self-control, and best preparation process is following:
(1) the hydrolysis substrate soybean protein mixes by weight 1:3 with brewer's yeast albumen.Add stirring and evenly mixing and become hydrolysis substrate solution.
(2) add acid protease (optimum pH that refers to enzyme is 3) in the said hydrolyzed substrate, the solid-to-liquid ratio of addition and protein hydrolysate substrate solution is 1:10, and the work of acid protease enzyme is preferably 5000U/g.
(3) hydrolysis substrate added behind the acid protease under 50 ℃ of conditions hydrolysis 2 hours, obtained hydrolysate, and product final pH value is 3.5 ~ 4, or it is also passable not regulate the pH value.
(4) the said hydrolyzed product adds preservative 0.5 ~ 2.0 weight portion again (calculating by 100 parts by weight with hydrolysate, is 100g like hydrolysate, then adds preservative 0.5 ~ 2.0g), adds pleocidin Spinosad 0.01 ~ 0.1 weight portion.Finally prepare said composite hydrolysis albumen.
Above-mentioned soybean protein is meant: the three-dimensional soybean protein in Shandong Co., Ltd produces, and is to be a kind of full price protide food additives of raw material production with the big dregs of beans of low temperature desolventizing.Protein content wherein: 68% ~ 70%, fat 1%, moisture 10%.
Brewer's yeast albumen is meant: brewery produces by the Zhujiang River, is to be raw material with the draft beer yeast, the animals ' protein forage that utilizes biological new and high technology and modern crafts to process.
Acid protease (Acid proteinase): unit of activity 100,000 U/g, the effective pH of enzyme is 2 ~ 4.0, effective temperature is 35-60 ℃.(Nanning east permanent magnificent road biotechnology Co., Ltd)
Preservative is meant Sodium Benzoate.
Among the present invention, molasses are meant that in the sugar industry process, remaining by product is uncrystallizable behind the crystallization of sucrose, but still contain the liquid residue than polysaccharide, are a kind of thickness, pitchy, are semifluid object.
The fruit essence addition is 1% in the final mass percentage concentration of compound attractant solution preferably.
Fruit essence is meant the volatile essential oil that fruit obtains through distillation extraction.Fruit essence is preferably carambola essence, mango essence, flavoring pineapple essence, guava essence, flavoring banana essence or orange flavor.In above-mentioned several kinds of essence, best with the attractant effect of flavoring pineapple essence.
Above-mentioned molasses water solution best in quality percentage concentration is 50%, and composite hydrolysis protein solution mass percentage concentration the best is 60%.
Compared with prior art, the present invention has following beneficial effect:
The compound attractant prescription of citrus fruit fly of the present invention is simple, and low in raw material cost is easy to get, and is very good to the female heavy attractant effect of citrus fruit fly.Show that through experiment the compound attractant of this citrus fruit fly is to the citrus fruit fly of 10 ~ 11 ages in days, the rate of always luring is more than 25%.The raw material that the compound attractant of citrus fruit fly of the present invention uses does not contain any harmful chemical, to humans and animals nonhazardous effect, compliance with environmental protection requirements.
Description of drawings
Fig. 1. contain the attracting action of the compound attractant of different proportioning flavoring pineapple essences to citrus fruit fly; Wherein CK is not for adding the blank of any essence, and the same letter on the post figure representes that different disposal lures rate through variance analysis difference not significantly (DMRT) on 0.05 level to female worm or male worm.
Fig. 2. compound attractant of the present invention and Sheng Tang Company products are lured rate (* * represent two kinds of attractants lure rate relatively through t-check significant difference on 0.01 level) relatively to citrus fruit fly.
Embodiment
The used molasses of following examples are provided by Guangzhou millipede sugar industry Co., Ltd, and fruit essence is provided by the Guangzhou City Meiyi Spice Co., Ltd.
Preparation composite hydrolysis albumen:
(1) the hydrolysis substrate soybean protein mixes by weight 1:3 with brewer's yeast albumen.Add the water stirring and evenly mixing and become hydrolysis substrate solution.
(2) add acid protease (optimum pH that refers to enzyme is 3) in the said hydrolyzed substrate, the solid-to-liquid ratio of addition and protein hydrolysate substrate solution is 1:10, and the work of acid protease enzyme is preferably 5000U/g.
(3) hydrolysis substrate added behind the acid protease under 50 ℃ of conditions hydrolysis 2 hours, obtained hydrolysate, and product final pH value is 3.5 ~ 4, or it is also passable not regulate the pH value.
(4) the said hydrolyzed product adds preservative 0.5 ~ 2.0 weight portion again (calculating by 100 parts by weight with hydrolysate, is 100g like hydrolysate, then adds preservative 0.5 ~ 2.0g), adds pleocidin Spinosad 0.01 ~ 0.1 weight portion.Finally prepare said composite hydrolysis albumen.
Being 50% molasses water solution with mass percentage concentration is mixed into the protein hydrolysate molasses mixture with 60% composite hydrolysis protein solution according to the ratio of 4: 1 quality.Add various fruit essences and make the shared mass fraction of essence be 4%, obtain compound attractant.
The results showed that the compound attractant that obtains according to optimization of C in the present embodiment is better than other prescriptions to the attracting action of citrus fruit fly adult.Test data is seen table 1 ~ 3.The result of table 1-3 shows; Compare with composite hydrolysis albumen+molasses; Though the compound attractant of composite hydrolysis albumen+molasses+fruit essence does not show stronger attracting action to the citrus fruit fly in 7 ages in days, stronger attractant effect is arranged for the adult behind 10 ages in days (egg-laying period).The result of Fig. 1 shows in addition, and the proportioning of adjustment essence can further improve the attractant effect of compound attractant, and the shared mass fraction of flavoring pineapple essence is that 1% o'clock rate of luring to the male and female worm all is higher than other concentration, and is wherein especially obvious to the attractant effect of female worm; The presentation of results of Fig. 2 is compared with the attractant of commercially available Sheng Tang company and is had more attracting action.
The compound attractant of table 1 is to the attracting action of citrus fruit fly (2 ~ 3 age in days)
| Compound attractant | Male worm is lured rate (%) | Female worm is lured rate (%) | Always lure rate (%) |
| Carambola essence+composite hydrolysis albumen+molasses | 13.33±0.96b | 13.89±1.11bc | 13.65±0.70b |
| Mango essence+composite hydrolysis albumen+molasses | 4.44±0.56c | 3.89±0.56e | 4.17±0.48d |
| Flavoring pineapple essence+composite hydrolysis albumen+molasses | 20.56±2.00a | 22.22±2.42a | 21.39±0.74a |
| Guava essence+composite hydrolysis albumen+molasses | 7.22±2.22c | 10.00±2.55cd | 8.61±2.37c |
| Flavoring banana essence+composite hydrolysis albumen+molasses | 8.89±0.56bc | 10.00±0.97cd | 9.45±0.28c |
| Orange flavor+composite hydrolysis albumen+molasses | 13.33±1.93b | 6.11±1.11de | 9.72±1.47c |
| Composite hydrolysis albumen+molasses | 21.67±1.93a | 18.89±2.42ab | 20.28±0.28a |
Same letter is represented through variance analysis difference not significantly (DMRT) on 0.05 level after the same column data, and the shared mass fraction of fruit essence is 4% (down with).
The compound attractant of table 2 is to the attracting action of citrus fruit fly (6 ~ 7 age in days)
| Compound attractant | Male worm is lured rate (%) | Female worm is lured rate (%) | Always lure rate (%) |
| Carambola essence+composite hydrolysis albumen+molasses | 11.89±0.11b | 12.83±0.60ab | 12.76±0.32a |
| Mango essence+composite hydrolysis albumen+molasses | 12.33±1.45b | 8.50±0.76b | 10.61±0.61ab |
| Flavoring pineapple essence+composite hydrolysis albumen+molasses | 11.11±1.11bc | 15.50±2.29a | 12.90±1.84a |
| Guava essence+composite hydrolysis albumen+molasses | 6.61±1.33d | 9.83±1.30b | 8.15±1.33b |
| Flavoring banana essence+composite hydrolysis albumen+molasses | 8.50±0.76cd | 8.50±1.80b | 8.45±1.27b |
| Orange flavor+composite hydrolysis albumen+molasses | 13.28±0.43b | 11.33±1.33ab | 12.32±0.73a |
| Composite hydrolysis albumen+molasses | 16.44±0.99a | 10.50±1.89ab | 13.60±1.38a |
The compound attractant of table 3 is to the attracting action of citrus fruit fly (10 ~ 11 age in days)
| Compound attractant | Male worm is lured rate (%) | Female worm is lured rate (%) | Always lure rate (%) |
| Carambola essence+composite hydrolysis albumen+molasses | 11.11±2.42b | 12.78±1.11bc | 11.94±1.39b |
| Mango essence+composite hydrolysis albumen+molasses | 9.44±1.11b | 6.67±0.96d | 8.06±0.73bc |
| Flavoring pineapple essence+composite hydrolysis albumen+molasses | 11.11±0.56b | 21.11±2.00a | 16.11±0.74a |
| Guava essence+composite hydrolysis albumen+molasses | 7.22±1.47b | 10.00±1.67bcd | 8.61±1.00bc |
| Flavoring banana essence+composite hydrolysis albumen+molasses | 24.45±4.01a | 11.11±2.78bcd | 17.78±2.74a |
| Orange flavor+composite hydrolysis albumen+molasses | 8.33±0.96b | 15.00±0.96b | 11.67±0.48b |
| Composite hydrolysis albumen+molasses | 5.00±0.96b | 7.78±1.47cd | 6.39±1.11c |
Claims (6)
1. the compound attractant of citrus fruit fly is characterized in that being made up of molasses water solution, composite hydrolysis protein solution and fruit essence, and the mass ratio of said molasses water solution and composite hydrolysis protein solution is 4:1;
Said composite hydrolysis albumen is that soybean protein is mixed by weight 1:1 ~ 9 with brewer's yeast albumen; Add acid protease 5000U/g; The product that hydrolysis obtained in 2 hours under 50 ℃ of conditions wherein also adds preservative 0.5 ~ 5.0 weight portion, pleocidin Spinosad 0.01 ~ 0.1 weight portion.
2. according to the compound attractant of the said citrus fruit fly of claim 1, it is characterized in that the mass percentage concentration of said fruit essence in compound attractant is 1%.
3. according to the compound attractant of the said citrus fruit fly of claim 1, it is characterized in that said fruit essence is the volatile essential oil that obtains through distillation extraction with fruit.
4. according to the compound attractant of the said citrus fruit fly of claim 3, it is characterized in that said fruit essence is carambola essence, mango essence, flavoring pineapple essence, guava essence, flavoring banana essence or orange flavor.
5. according to the compound attractant of the said citrus fruit fly of claim 1, the mass percentage concentration that it is characterized in that molasses in the molasses water solution is 50%.
6. according to the compound attractant of the said citrus fruit fly of claim 1, the mass percentage concentration that it is characterized in that composite hydrolysis albumen in the composite hydrolysis protein solution is 60%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304337A (en) * | 2014-09-05 | 2015-01-28 | 沧州科惠物理防虫研究所 | Plant source biotype fruit fly bisexual trapping and killing agent |
| CN104996485A (en) * | 2015-08-13 | 2015-10-28 | 广东省昆虫研究所 | Bactrocera dorsalis slow-release type attractant |
| CN111109306A (en) * | 2019-12-06 | 2020-05-08 | 四川省农业科学院植物保护研究所 | Method for preventing and treating bactrocera dorsalis |
| CN118240829A (en) * | 2024-05-21 | 2024-06-25 | 华南农业大学 | Application of NompC gene in control of bactrocera dorsalis |
Citations (2)
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|---|---|---|---|---|
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN101697739A (en) * | 2009-10-27 | 2010-04-28 | 华南农业大学 | bactrocera dorsalis hendel attractant |
-
2011
- 2011-10-11 CN CN2011103061747A patent/CN102499269A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN101697739A (en) * | 2009-10-27 | 2010-04-28 | 华南农业大学 | bactrocera dorsalis hendel attractant |
Non-Patent Citations (3)
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| 申建梅,等: "香蕉果实挥发物的化学成分比较分析", 《环境昆虫学报》 * |
| 胡黎明,等: "桔小实蝇对甜橙精油的行为反应及精油化学成分分析", 《广东农业科学》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304337A (en) * | 2014-09-05 | 2015-01-28 | 沧州科惠物理防虫研究所 | Plant source biotype fruit fly bisexual trapping and killing agent |
| CN104304337B (en) * | 2014-09-05 | 2017-10-10 | 沧州佳和生物科技有限公司 | Plant source bion trypetid both sexes attractant |
| CN104996485A (en) * | 2015-08-13 | 2015-10-28 | 广东省昆虫研究所 | Bactrocera dorsalis slow-release type attractant |
| CN111109306A (en) * | 2019-12-06 | 2020-05-08 | 四川省农业科学院植物保护研究所 | Method for preventing and treating bactrocera dorsalis |
| CN118240829A (en) * | 2024-05-21 | 2024-06-25 | 华南农业大学 | Application of NompC gene in control of bactrocera dorsalis |
| CN118240829B (en) * | 2024-05-21 | 2024-07-23 | 华南农业大学 | Application of NompC gene in control of bactrocera dorsalis |
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Application publication date: 20120620 |