CN101066096A - Artificial raising of orange fly larva - Google Patents

Artificial raising of orange fly larva Download PDF

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Publication number
CN101066096A
CN101066096A CNA2007100273511A CN200710027351A CN101066096A CN 101066096 A CN101066096 A CN 101066096A CN A2007100273511 A CNA2007100273511 A CN A2007100273511A CN 200710027351 A CN200710027351 A CN 200710027351A CN 101066096 A CN101066096 A CN 101066096A
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CN
China
Prior art keywords
citrus fruit
fruit fly
man
made feeds
fly larva
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Pending
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CNA2007100273511A
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Chinese (zh)
Inventor
田世尧
阎青云
曾杨
潘建平
林志雄
匡石滋
高强
杨长举
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Pomology Research Institute Guangdong Academy of Agricultural Sciences
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Pomology Research Institute Guangdong Academy of Agricultural Sciences
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Application filed by Pomology Research Institute Guangdong Academy of Agricultural Sciences filed Critical Pomology Research Institute Guangdong Academy of Agricultural Sciences
Priority to CNA2007100273511A priority Critical patent/CN101066096A/en
Publication of CN101066096A publication Critical patent/CN101066096A/en
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Abstract

The present invention discloses artificial feed for citrus fruit fry. The artificial feed is mixture of corn powder 40-48 weight portions, yeast powder 5-10 weight portions, adult oviposition attractant 0.5-1 weight portions, hydrochloric acid 0.01-0.5 weight portions, stuffing 2-5 weight portions, preservative 0.01-0.5 and water 80-90 weight portions. Its adult oviposition attractant can attract female citrus fruit fry to lay eggs for easy collection of citrus fruit fry eggs. The present invention makes it possible to feed great amount of citrus fruit fry of different generations in low cost and without limitation of seasons.

Description

The man-made feeds of citrus fruit fly larva
1, technical field:
The present invention relates to the man-made feeds of a kind of citrus fruit fly larva.
2, background technology:
Citrus fruit fly is commonly called as fruit and chews, and is a kind of universal crop pests, belongs to two class quarantine pest insects, mainly is distributed in south China and southwest in China.Its adult lays eggs in pericarp, larva eats into food in pulp, often cause fruit to rot or underdone yellow earlier and come off, have a strong impact on the output and the quality of fruit, that have even lost edibility, therefore the nearly kind more than 200 of its fruit of causing harm seeks the effectively preventing method, has become important problem both at home and abroad.
In recent years, owing to deepening continuously of entomology research, and the development of control of insect technology, need be supplied in the standardized insect of ten thousand more than one hundred million physiology.
At present, the worm source of citrus fruit fly is mainly from natural raising, but the large-scale citrus fruit fly of natural raising, the cost height easily pollutes, and is subject to seasonal restrictions.And adopt the artificial feeding citrus fruit fly to have huge superiority, and can Artificial Control raise condition, be convenient at awful weather particularly breeding in a large number in the winter environment.It is whole that artificial feeding not only can make insect grow, the physiology unanimity, and be the main path that solves seasonal feed shortage under many circumstances, because most of natural foodstuffs can not utilize, only reliable artificial feeding just can make research continue as winter.But the research of the man-made feeds of citrus fruit fly still relatively lacks at present.
3, summary of the invention:
In order to overcome the deficiencies in the prior art, the purpose of this utility model provides the man-made feeds of a kind of citrus fruit fly larva, and it is the mass rearing citrus fruit fly manually.
The present invention is achieved by the following technical solution:
The man-made feeds of a kind of citrus fruit fly larva is characterized in that: it includes the following raw material system of mixing and forms (in weight portion):
Corn flour: 40~48; Dusty yeast: 5~10; Adult oviposition attractant: 0.5~1; Hydrochloric acid: 0.01~0.5; Filler: 2~5; Anticorrisive agent: 0.01~0.5; Water: 80~90.
In each composition of feed, contents of saccharide is abundant in the corn flour, and is rich in protein and more vitamin, in the artificial feeding of citrus fruit fly, getting of this worm is eaten and the good facilitation of growing.And convenient, cheap, the consumption economy of originating.Dusty yeast can not only satisfy the needs of insect to the B family vitamin, various inorganic salts and rich in protein and carbohydrate etc. can also be provided, can increase considerably the protein content of female pupa within the specific limits, improve the reproductive capacity of adult, and dusty yeast can make feed loose, is convenient to insect's food-taking.The effect of hydrochloric acid is the pH value of regulating man-made feeds, to suppress bacterium and fungi growth.Therefore this feed can utilize the adult oviposition attractant to lure the female adult of citrus fruit fly to land to lay eggs in the above in larva artificial feed, with in gathering bactrocera dorsalis ovum, and be beneficial to the larval feeding that ovum hatches out, can realize a large amount of each citrus fruit fly from generation to generation of artificial feeding.
Above-mentioned described scheme can be further improved by following measure:.
The weight ratio of described each raw material is: corn flour: 45, and dusty yeast: 7, the adult oviposition attractant: 0.88, hydrochloric acid: 0.24, filler: 3, anticorrisive agent: 0.1, water: 85.
Described adult oviposition attractant is a kind of of ethyl acetate, ethyl butyrate, carbonic hydroammonium, phenylmethyl acetic acid, ethyl benzoate.
Described adult oviposition attractant is to be mixed by ethyl butyrate and ethyl benzoate, and the weight ratio of ethyl butyrate and ethyl benzoate is: 7: 3.
Described anticorrisive agent is sorbic acid, Ni Baijin, formaldehyde, benzoic a kind of.
Described dusty yeast is a beer yeast powder.
Described filler is a paper handkerchief.The paper handkerchief that adds appropriate amount in the citrus fruit fly man-made feeds satisfies cellulosic demand, and cellulose is as a kind of filler, to the effect of the helpful digestion of phytophagous citrus fruit fly.
The present invention compared with prior art has following advantage: 1) can realize a large amount of each citrus fruit fly from generation to generation of artificial feeding; 2) can Artificial Control raise condition, not be subjected to seasonal the influence; 3) the source facility of main component such as corn flour is cheap, economical and practical, and prepares easy; 4) contain anticorrisive agent, feed undergoes no deterioration in feeding period, and is also pollution-free.
4, the specific embodiment:
Man-made feeds of the present invention are to be formed by the following raw material system of mixing, and the weight ratio of described each raw material is: corn flour: 45, and beer yeast powder: 7, the adult oviposition attractant: 0.88, concentrated hydrochloric acid: 0.24, cellulose: 3, Ni Bojin: 0.1, water: 85.Wherein water is distilled water, and the adult oviposition attractant is to be formed by ethyl butyrate and the ethyl benzoate system of mixing, and the weight ratio of ethyl butyrate and ethyl benzoate is: 7: 3.Above raw material blending promptly can be made into the man-made feeds of citrus fruit fly larva after autoclaving also cools off.
Present embodiment employing concentration is 36.47% hydrochloric acid.Control the pH value between 4~5.5, like most this scope citrus fruit fly and get food.
By respectively each the worm attitude of citrus fruit fly under man-made feeds and the natural feed raising being observed, note the similarities and differences of two kinds of each worm attitude life habits under the feeds raising, and draw some biological indicators shown in the table 1, wherein selecting banana is the citrus fruit fly natural feed.Test is carried out under the condition of temperature T=25~28 ℃, relative humidity RH=80%.
Table 1 citrus fruit fly accumulated for 5 generations and raises the result
From generation to generation Man-made feeds are raised
Examination borer population (head) Egg hatching rate (%) Survival rate of larvae (%) Average pupa heavy (mg) Percentage of pupation (%) Eclosion rate (%)
1 486 75.0 85.3 15.30 96.6 98.0
2 500 77.8 84.5 15.22 92.2 97.4
3 500 75.8 87.4 15.25 95.5 94.0
4 500 76.4 86.5 15.18 94.0 95.0
5 500 75.0 83.0 15.15 95.2 94.2
Mean value 496 76.0 85.1 15.22 94.7 95.7
Natural raising 500 81.4 90.1 15.12 97.6 98.2
More than the method for each index test as follows:
1) egg hatching rate is measured
200 citrus fruit fly adults (female: male=1: 1) that the back 15 days first generation man-made feeds of sprouting wings are raised are put into a cage (50 * 50 * 50cm), put into ready-made 3 ware larva artificial feeds again and adopt ovum, the record egg laying amount, calculate the egg hatching rate of every batch of ovum, so adopted a deutovum every 10 days, adopt altogether 5 times, obtain the average incubation rate of ovum.
It under similarity condition, serve as that same test, egg hatching rate computing formula are carried out in contrast: egg hatching rate=(newly hatched larvae number/confession tries the ovum sum) * 100 with the banana raising.
2) survival rate of larvae is measured
Choose 600 newly hatched larvaes, if three repetitions, per 200 is that a processing is received respectively on the citrus fruit fly larva artificial feed, note the mature larva quantity before pupating, ask its average, utilize formula to calculate the larva survival rate: larva survival rate=(mature larva/confession examination larva sum) * 100.
3) percentage of pupation is measured
Choose the larva in the above survival rate of larvae determination test, it is added up and observes, write down three groups of pupa numbers of pupating in repeating respectively, calculate percentage of pupation: percentage of pupation=(pupa number/confession examination larva number) * 100 according to formula.
4) average pupa is heavy:
Collect the pupa of being changed in the above percentage of pupation test, being placed in fills in the glass tube that water content is the 5%-10% sand, 30 pupas of every pipe.Note from mature larva and begin to pupate to finish the weighted average of institute's elapsed-time standards, be pupa time to adult eclosion.Mature larva go into husky through 45 hours prepupal period after, the pupa of being changed is weighed, asking for average, to be the average pupa of every pupa heavy.
5) eclosion rate is measured:
After measuring percentage of pupation, the pupa branch is installed in the sand glass tube that 5~10% moisture are housed, handle and contrast and respectively establish 3 repetitions, 30 of every pipes are preserved in the dark, the adult number that record is sprouted wings utilizes formula to calculate: eclosion rate=(sprouting wings into borer population/confession examination pupa number) * 100.
Can find out continuous 5 generations citrus fruit flies some biological indicators in raising from table 1: egg hatching rate=76.0%, survival rate of larvae=84.1%, average pupa is heavy=15.22mg, percentage of pupation=94.7%, eclosion rate=95.7%.The index of the raising of these indexs and natural feed is comparatively approaching, therefore can utilize a large amount of citrus fruit fly of this feed artificial feeding, and the research that can be subjects such as its biology, physiology, pathology, toxicology and science of heredity provides sufficient worm source.
Above-mentioned anticorrisive agent can also adopt sorbic acid, formaldehyde or benzoic a kind of.
The preparation that above-mentioned adult oviposition attractant can also adopt one or more mixed preparing of ethyl acetate, ethyl butyrate, carbonic hydroammonium, phenylmethyl acetic acid, ethyl benzoate to form.

Claims (7)

1, the man-made feeds of a kind of citrus fruit fly larva is characterized in that: it includes the following raw material system of mixing and forms (in weight portion):
Corn flour: 40~48; Dusty yeast: 5~10; Adult oviposition attractant: 0.5~1; Hydrochloric acid: 0.01~0.5; Filler: 2~5; Anticorrisive agent: 0.01~0.5; Water: 80~90.
2, the man-made feeds of citrus fruit fly larva according to claim 1 is characterized in that: the weight ratio of described each raw material is: corn flour: 45, and dusty yeast: 7, the adult oviposition attractant: 0.88, hydrochloric acid: 0.24, filler: 3, anticorrisive agent: 0.1, water: 85.
3, the man-made feeds of citrus fruit fly larva according to claim 1 and 2 is characterized in that: the adult oviposition attractant is a kind of of ethyl acetate, ethyl butyrate, carbonic hydroammonium, phenylmethyl acetic acid, ethyl benzoate.
4, the man-made feeds of citrus fruit fly larva according to claim 1 and 2 is characterized in that: described adult oviposition attractant is to be mixed by ethyl butyrate and ethyl benzoate, and the weight ratio of ethyl butyrate and ethyl benzoate is: 7: 3.
5, the man-made feeds of citrus fruit fly larva according to claim 1 and 2 is characterized in that: described anticorrisive agent is sorbic acid, Ni Baijin, formaldehyde, benzoic a kind of.
6, the man-made feeds of citrus fruit fly larva according to claim 1 and 2 is characterized in that: described dusty yeast is a beer yeast powder.
7, the man-made feeds of citrus fruit fly larva according to claim 1 and 2 is characterized in that: described filler is a paper handkerchief.
CNA2007100273511A 2007-03-30 2007-03-30 Artificial raising of orange fly larva Pending CN101066096A (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101433276B (en) * 2008-12-22 2011-10-05 山东省农业科学院植物保护研究所 Artificial synthetic feed containing leek maggot
CN102630838A (en) * 2012-04-27 2012-08-15 中国计量学院 Preparation scheme and method of citrus fruit fly larva feed
CN101697739B (en) * 2009-10-27 2013-05-01 华南农业大学 Bactrocera dorsalis hendel attractant
CN103300257A (en) * 2013-07-09 2013-09-18 海南出入境检验检疫局热带植物隔离检疫中心 Artificial feed for bactrocera dorsalis larvas
CN106689826A (en) * 2016-12-05 2017-05-24 长沙秋点兵信息科技有限公司 Ceratitis capitata larva feed capable of shortening hatching period
CN107439545A (en) * 2017-08-13 2017-12-08 中国农业科学院植物保护研究所 A kind of lure composition and its application for being used to prevent and treat black cutworm
CN109526960A (en) * 2018-12-14 2019-03-29 大连理工大学 A kind of inducing agent and its application method based on black soldier flies larva olfactory information element
CN111141874A (en) * 2019-03-27 2020-05-12 广西壮族自治区植保站 Method for measuring trapping and killing effect of bactrocera dorsalis adults
CN115886160A (en) * 2022-11-30 2023-04-04 华南农业大学 Feed for larvae of Bactrocera dorsalis in south Asia
CN116019105A (en) * 2022-12-26 2023-04-28 中国农业科学院果树研究所 Composition for improving oviposition of peach fruit borers
CN117617362A (en) * 2024-01-12 2024-03-01 贵州省蚕业研究所(贵州省辣椒研究所) Application of sodium butyrate in improving cocoon yield

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101433276B (en) * 2008-12-22 2011-10-05 山东省农业科学院植物保护研究所 Artificial synthetic feed containing leek maggot
CN101697739B (en) * 2009-10-27 2013-05-01 华南农业大学 Bactrocera dorsalis hendel attractant
CN102630838A (en) * 2012-04-27 2012-08-15 中国计量学院 Preparation scheme and method of citrus fruit fly larva feed
CN103250899A (en) * 2012-04-27 2013-08-21 中国计量学院 Citrus fruit fly artificial-feed and preparation method thereof
CN103300257A (en) * 2013-07-09 2013-09-18 海南出入境检验检疫局热带植物隔离检疫中心 Artificial feed for bactrocera dorsalis larvas
CN106689826A (en) * 2016-12-05 2017-05-24 长沙秋点兵信息科技有限公司 Ceratitis capitata larva feed capable of shortening hatching period
CN107439545A (en) * 2017-08-13 2017-12-08 中国农业科学院植物保护研究所 A kind of lure composition and its application for being used to prevent and treat black cutworm
CN107439545B (en) * 2017-08-13 2020-02-18 中国农业科学院植物保护研究所 Attractant composition for preventing and treating black cutworm and application thereof
CN109526960A (en) * 2018-12-14 2019-03-29 大连理工大学 A kind of inducing agent and its application method based on black soldier flies larva olfactory information element
CN111141874A (en) * 2019-03-27 2020-05-12 广西壮族自治区植保站 Method for measuring trapping and killing effect of bactrocera dorsalis adults
CN115886160A (en) * 2022-11-30 2023-04-04 华南农业大学 Feed for larvae of Bactrocera dorsalis in south Asia
CN115886160B (en) * 2022-11-30 2024-05-28 华南农业大学 Nanya fruit fly larva feed
CN116019105A (en) * 2022-12-26 2023-04-28 中国农业科学院果树研究所 Composition for improving oviposition of peach fruit borers
CN116019105B (en) * 2022-12-26 2024-05-24 中国农业科学院果树研究所 Composition for improving oviposition of peach fruit borers
CN117617362A (en) * 2024-01-12 2024-03-01 贵州省蚕业研究所(贵州省辣椒研究所) Application of sodium butyrate in improving cocoon yield

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Open date: 20071107