CN101965822B - Method for artificially breeding mass Carpoglyphus lactis - Google Patents
Method for artificially breeding mass Carpoglyphus lactis Download PDFInfo
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- CN101965822B CN101965822B CN201010524994.9A CN201010524994A CN101965822B CN 101965822 B CN101965822 B CN 101965822B CN 201010524994 A CN201010524994 A CN 201010524994A CN 101965822 B CN101965822 B CN 101965822B
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000009395 breeding Methods 0.000 title claims abstract description 24
- 230000001488 breeding effect Effects 0.000 title claims abstract description 24
- 241000260546 Carpoglyphus lactis Species 0.000 title claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000238876 Acari Species 0.000 claims abstract description 14
- 235000000346 sugar Nutrition 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 22
- 230000000384 rearing effect Effects 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 230000003442 weekly effect Effects 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 235000021552 granulated sugar Nutrition 0.000 claims description 2
- 239000010451 perlite Substances 0.000 claims description 2
- 235000019362 perlite Nutrition 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 238000009923 sugaring Methods 0.000 claims description 2
- 239000002023 wood Substances 0.000 claims description 2
- -1 wooden Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000003715 nutritional status Nutrition 0.000 abstract description 2
- 230000017448 oviposition Effects 0.000 abstract description 2
- 235000013312 flour Nutrition 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 241000254127 Bemisia tabaci Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241001206567 Neoseiulus barkeri Species 0.000 description 2
- 241001455175 Neoseiulus cucumeris Species 0.000 description 2
- 241000611866 Tyrophagus putrescentiae Species 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 241001553884 Aleuroglyphus ovatus Species 0.000 description 1
- 241001664794 Amblyseius swirskii Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention relates to a method for artificially breeding mass Carpoglyphus lactis. In the method, the Carpoglyphus lactis is bred by 1 to 50 percent of sugar, 1 to 50 percent of yeast, 0 to 50 percent of breeding medium and 0 to 35 percent of water in an environment with the temperature of between 15 and 35 DEG C and the relative humidity of 65 to 95 percent. The method comprises the following steps of: after combining breeding materials, throwing the combined breeding materials into a breeding container; inoculating the Carpoglyphus lactis, wherein the Carpoglyphus lactis is inoculated in an amount of more than or equal to one Carpoglyphus lactis per milliliter; and breeding the Carpoglyphus lactis for 10 to 40 days to realize mass breeding. The Carpoglyphus lactis is bred by the sugar, the yeast, the breeding medium and the water, so the Carpoglyphus lactis can be efficiently bred, the method is easy to operate and has low cost, the bred Carpoglyphus lactis has the characteristics of good nutritional status, low death rate, high egg laying amount, high population density and the like, and the method is more suitable for low-cost commercial production of predatory mites.
Description
Technical field
This invention relates to a kind of method of artificial mass rearing hesperidium mite [Carpoglyphus lactis (Linnaeus, 1758)].
Technical background
Hesperidium mite individuality is small, widely distributed, can be used for the raising of multiple Predatory Mites, the little blind peaceful mite (Typhlodromips swirskii) of Si Shi and domestic and international now commercial amblyseius cucumeris oudeman (Amblyseius cucumeris), Amblyseius barkeri (Amblyseius barkeri) as having fine control action to Bemisia tabaci (Bemisia tabaci) all can be raised with hesperidium mite.Hesperidium mite has important value in the scale of Predatory Mites and industrialization producer mask.
Hesperidium mite feeding habits compared with other flour mite are special, and can not work the mischief to the crops grown and Predatory Mites release environment, be a kind of feed prey of safety.Current most Predatory Mites with flour mite as tyrophagus putrescentiae (Tyrophagus putrescentiae), (Aleuroglyphus ovatu etc. are feed prey mass rearing to ellipse dispersion, simultaneously its subject matter be discharged in environment with Predatory Mites, potential harm is produced, when particularly quantity is larger to crop.If hesperidium mite replaces the raising Predatory Mites prey that flour mite becomes first-selected, then can reduce the harm again to release areas.Particularly in Edible Fungi Pests control, accompany the flour mite of putting definitely will forbid with Predatory Mites.
The hesperidium mite raising method now reported mainly concentrates on and utilizes Saccharomyces cerevisiae raise the method (patent publication No. CN101111148A, CN101111149A) of hesperidium mite and utilize feed (being generally ground rice, pollen) 5% ~ 60%, yeast 0.5% ~ 10%, vitamin 0.01% ~ 2%, sugar 0.5% ~ 10%, antibiotic 0.01 ~ 0.5% and medium to raise the method (patent publication No. CN101268772A) of hesperidium mite.There is the defect that feeding cost is high, growth rate is slow in the method for raising hesperidium mite in the first two publication (CN101111148A, CN101111149A), the Saccharomyces cerevisiae mentioned of the method brings a high price China is domestic, is not suitable as a kind of hesperidium mite breeding feed of cheapness; The method 22 DEG C ~ 25 DEG C, raise hesperidium mite under the relative humidities of 85 ~ 90%, quantity only increases by 2 ~ 4 times weekly usually, and hesperidium mite growth rate is slower.There is batching complexity, complex operation, cost is high, growth rate is slow defect in the method for raising hesperidium mite in a rear publication (CN101268772A), needed for the method, breeding feed constituent has 6 kinds, prepares more complicated; The method that the method every 1-7 days adds 1 defective material requires all to carry out continued operation weekly, operates relatively loaded down with trivial details; Pollen used, ground rice, vitamin and antibiotic cost are all very high, and the cheaper commodityization not being suitable for Predatory Mites is produced; Hesperidium mite raising method mentioned in this patent embodiment, under the condition of temperature 25 DEG C, relative moisture 75%, after being raised, reaches 300/g, illustrates 3 weeks and only breed 15 times by early stage 20/g through 3 weeks.
Summary of the invention
Object of the present invention is just to provide a kind of method of practicality, cheapness, the artificial mass rearing hesperidium mite that simple, workload is low.
The present invention is under the environment of temperature 15 ~ 35 DEG C, relative moisture 65 ~ 95%, utilizes sugar 1% ~ 50%, yeast 1% ~ 50%, raises the method for medium 0 ~ 50% and water 0 ~ 35% raising hesperidium mite, specifically comprise the following steps:
1) the getting the raw materials ready of hesperidium mite breeding feed: sugar is selected from glucose, cane suger, beet sugar or white granulated sugar, yeast is selected from brewer's yeast, fodder yeast, dried yeast or eats female raw cook, raise medium and be selected from wheat bran, vermiculite, perlite, rice bran, wheat bran, wood chip or crops straw crushed material, wet concentration is from running water, cold water or mineral water;
2) process of hesperidium mite breeding feed: sugar and yeast, through deinsectization process, raise medium through autoclave sterilization process;
3) preparation and the process of container is raised: raise the high 5 ~ 25cm of container, material is plastics, wooden, glass or irony, raise that container can seal completely, there is seal cover upper end, seal cover has ventilation mouth, ventilation mouth gauze, cloth or the paper sealing that can effectively stop hesperidium mite to escape, raises container through deinsectization sterilization treatment before using;
4) raising method of hesperidium mite: directly join after breeding feed is fully mixed and raise in container, or first sugaring adds water, yeast, medium more successively in raising container, breeding feed thickness be raise container high 1/5 ~ 4/5, preferably 3 ~ 8cm, hesperidium mite is accessed again after access breeding feed, hesperidium mite initially access amount >=1/ml, hesperidium mite or hesperidium mite can be added weekly raise and expect and raise in container, raise 10 ~ 40 days, namely hesperidium mite insect density >=100/ml can be used for the raising of Predatory Mites or continues to expand numerous raising.
The raising ratio of sugar, yeast, raising medium and water in hesperidium mite breeding feed used refers to mass ratio.
In hesperidium mite breeding feed used various get the raw materials ready selection source can separately, two kinds or two or more used in combination.
The present invention be utilize be easy to get, method that cheap sugar and yeast, raising medium, water raise hesperidium mite.The efficient raising of hesperidium mite can not only be realized, and simple to operate, cost is cheaper, support hesperidium mite there is the features such as nutritional status is good, lethality is low, egg laying amount is high, insect density is large, be more suitable for the merchandized handling of Predatory Mites.
Embodiment
Following instance is only the citing in the present invention, is not limitation of the present invention.
The artificial mass rearing method of embodiment 1 hesperidium mite
In the climatic cabinate of temperature 25 ± 1 DEG C, relative moisture 90 ± 1%, connect 200 hesperidium mites in the culture vessel containing white sugar 9g, the female raw cook of the food that grinds (calculating with dried yeast) 1g, wheat bran 7g, vermiculite 3g and water 5g, repeat 5 times, wherein raising container is the small size circular crisper of toy adopting Quanzhou, Fujian city Feng Zehongxing plastic cement everyday articles Co., Ltd to produce, process is by the partial removal under lid central fovea, and seal with 2 layer of 350 object nylon gauze, wheat bran and vermiculite use after 120 DEG C of deinsectization sterilizations 2 hours, cooling.Raised through 30 days, hesperidium mite has bred nearly 1400 times, illustrates that the method is the fine method (table 1) of one of raising hesperidium mite.
Embodiment 2 is anhydrous, without the method for mass rearing hesperidium mite under ambient condition
Under the condition of temperature 25 ± 1 DEG C, relative moisture 85 ± 5%, in the ratio mixing 20g of glucose, dried yeast mass ratio 1: 1, access 200 hesperidium mites, raise 20 days, hesperidium mite can expand numerous 100 times.
The raising proliferation results of table 1 hesperidium mite after 30 days
Note: in table, average data is mean+SD (means ± SD).
Claims (1)
1. the method for an artificial mass rearing hesperidium mite (Carpoglyphus lactis), it is characterized in that under the environment of temperature 15 ~ 35 DEG C, relative moisture 65 ~ 95%, sugar 36%, yeast 4%, raising medium 40% and water 20% is utilized to raise the method for hesperidium mite, the ratio of described sugar, yeast, raising medium and water refers to mass ratio, specifically comprises the following steps:
1) the getting the raw materials ready of hesperidium mite breeding feed: sugar is selected from glucose, cane suger, beet sugar, white granulated sugar, yeast is selected from brewer's yeast, fodder yeast, dried yeast, the female raw cook of food, raise medium and be selected from wheat bran, vermiculite, perlite, rice bran, wheat bran, wood chip, crops straw crushed material, wet concentration is from running water, cold water, mineral water, and in described breeding feed, various selection source of getting the raw materials ready refers to independent or two or more used in combination;
2) process of hesperidium mite breeding feed: sugar and yeast, through deinsectization process, raise medium through autoclave sterilization process;
3) preparation and the process of container is raised: raise the high 5 ~ 25cm of container, material is plastics, wooden, glass or irony, raise that container can seal completely, there is seal cover upper end, seal cover has ventilation mouth, ventilation mouth gauze, cloth or the paper sealing that can effectively stop hesperidium mite to escape, raises container through deinsectization sterilization treatment before using;
4) raising method of hesperidium mite: directly join after breeding feed is fully mixed and raise in container, or first sugaring adds water, yeast, medium more successively in raising container, breeding feed thickness be raise container high 1/5 ~ 4/5, hesperidium mite is accessed again after access breeding feed, hesperidium mite initially access amount >=1/ml, interpolation hesperidium mite or hesperidium mite are raised and expect in raising container weekly, raise 10 ~ 40 days, namely hesperidium mite density >=100/ml can be used for the raising of Predatory Mites or continues to expand numerous raising.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010524994.9A CN101965822B (en) | 2010-10-29 | 2010-10-29 | Method for artificially breeding mass Carpoglyphus lactis |
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| CN201010524994.9A CN101965822B (en) | 2010-10-29 | 2010-10-29 | Method for artificially breeding mass Carpoglyphus lactis |
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| CN101965822A CN101965822A (en) | 2011-02-09 |
| CN101965822B true CN101965822B (en) | 2015-02-11 |
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| CN103299958A (en) * | 2013-05-20 | 2013-09-18 | 中国农业科学院植物保护研究所 | Novel method for feeding prey flour mites and predatory mites |
| CN103299960B (en) * | 2013-05-20 | 2016-04-27 | 中国农业科学院植物保护研究所 | The artificial mass rearing method of thin mouth mite |
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| CN108633842A (en) * | 2018-04-10 | 2018-10-12 | 首伯农(北京)生物技术有限公司 | A method of raising tarsonemid |
| FR3081678B1 (en) * | 2018-06-01 | 2021-10-29 | Ynsect | LAYING MEDIA FOR INSECTS CONTAINING A SOLID SUBSTRATE |
| FR3081679B1 (en) * | 2018-06-01 | 2021-11-26 | Ynsect | LAYING MEDIA FOR INSECTS CONTAINING A TEXTURING SUBSTRATE |
| CN111543397A (en) * | 2020-06-23 | 2020-08-18 | 安徽国际旅行卫生保健中心(合肥海关口岸门诊部) | Rapid propagation culture method for Tyrophagus putrescentiae |
| CN111543398A (en) * | 2020-06-24 | 2020-08-18 | 潮州中天农业科技有限公司 | Predatory mite and prey mite feeding method |
| CN111685082B (en) * | 2020-07-01 | 2023-01-13 | 福建农林大学 | Method for artificially and compositely feeding bacteria-macrochelia and dygorgia serrulata |
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Inventor after: Wang Boming Inventor after: Dong Jie Inventor after: Qiao Yan Inventor after: Wang Weiqing Inventor after: Wu Shengyong Inventor after: Jiang Junqi Inventor after: Wang Beibei Inventor after: Xu Xuenong Inventor after: Jiang Xiaohuan Inventor after: Fan Qinghai Inventor after: Wang Endong Inventor after: Lv Jiale Inventor after: Luo Jia Inventor after: Wu Meixiang Inventor after: Li Guiting Inventor before: Wang Boming Inventor before: Wang Beibei Inventor before: Xu Xuenong Inventor before: Jiang Xiaohuan Inventor before: Fan Qinghai Inventor before: Wang Endong Inventor before: Luo Jia Inventor before: Wu Meixiang Inventor before: Li Guiting Inventor before: Wu Shengyong |
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Free format text: CORRECT: INVENTOR; FROM: WANG BOMING XU XUENONG JIANG XIAOHUAN FAN QINGHAI WANG ENDONG LUO JIA WU MEIXIANG LI GUITING WU SHENGYONG WANG BEIBEI TO: WANG BOMING XU XUENONG JIANG XIAOHUAN FAN QINGHAI WANG ENDONG LV JIALE LUO JIA WU MEIXIANG LI GUITING DONG JIE QIAO YAN WANG WEIQING WU SHENGYONG JIANG JUNQI WANG BEIBEI |
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Effective date of registration: 20141202 Address after: 100193 Beijing Old Summer Palace West Road, Haidian District, No. 2 Applicant after: Institute of Plant Protection, Chinese Academy of Agricultural Sciences Applicant after: SHOUBONONG (BEIJING) BIOTECHNOLOGY CO., LTD. Applicant after: Anhui Agricultural University Address before: 100193 Beijing Old Summer Palace West Road, Haidian District, No. 2 Applicant before: Institute of Plant Protection, Chinese Academy of Agricultural Sciences Applicant before: Wang Boming |
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