CN102511452B - Novel method for artificial mass rearing of Amblyseius orientalis - Google Patents
Novel method for artificial mass rearing of Amblyseius orientalis Download PDFInfo
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Abstract
The invention relates to a novel method for artificial mass rearing of Amblyseius orientalis, which is a method for artificial mass rearing of Amblyseius orientalis using Carpoglyphus lactis or composition of the Carpoglyphus lactis with other food. The other food, exclusive of the Carpoglyphus lactis, includes mites in reverse materials, eggs of insects, pollen, sugar, honey, yeast and the like. Using the method solves the problem that Amblyseius orientalis is difficult to rear and high in rearing cost and can only be reared with mites, pollen and honey water for long. The method has the advantages that the method breaks through predecessors' misconception that Amblyseius orientalis is a type of strict predatory mites, unpredicted technical effects are achieved, using the brand-new rearing scheme achieves simple, low-cost and efficient mass rearing of Amblyseius orientalis, a new era of development and utilization of Amblyseius orientalis is started, more and more efficient native natural enemies can be provided for safety production of agriculture and forestry products, and positive contribution is made to environmental safety.
Description
Technical field
This invention relates to the new method of a kind of artificial mass rearing Amblyseius Orientalis Ehara (Amblyseius orientalis), belongs to the biological prevention field of pest.
Technical background
Amblyseius Orientalis Ehara belongs to Phytoseiidae (Phytoseiidae), genus amblyseius berlese subfamily (Amblyseiinae), Amblysius (Amblyseius), can effectively prevent and treat citrus red mite (crm) (Panonychus citri), panonychus ulmi (Panonychusulmi), tetranychus viennensis (Tetranychus viennensis), cypress unguiculus mite (Oligonychus perditus) etc., be the considerable advantage kind Predatory Mites in Control of Pest Mites.
The advantage that Amblyseius Orientalis Ehara also has is as follows:
Amblyseius Orientalis Ehara is a kind of effective Dominant Species of Natural Enemies in Citrus Orchard
[1], fine to the control effect of citrus red mite.Yang Ziqi etc.
[2]on tangerine tree, discharge the test of Amblyseius Orientalis Ehara control citrus red mite and show, discharge 5 days and check afterwards, citrus red mite (crm) insect population declines and reaches 68.86%; Very remarkable to tetranychid control action on apple tree.Zhang Shouyou etc.
[3]at apple orchard release Amblyseius Orientalis Ehara control panonychus ulmi and tetranychus viennensis test, show, in natural situation, Amblyseius Orientalis Ehara can be controlled these two kinds harmful mites completely, and control efficiency reaches 93.4%.
With two kinds of large-scale production specially the Predatory Mites of food tetranychids the genus amblyseius berlese (Amblyseiuspseudolongispinosus) of intending becoming mildewed compare with the genus amblyseius berlese that becomes mildewed (Amblyseius longispinosus); Amblyseius Orientalis Ehara is suitable with it to the predation amount of tetranychid, and this has shown the powerful prey ability that Amblyseius Orientalis Ehara has equally to tetranychid.Research shows, at 24-28 ℃, citrus red mite (crm), becoming mite amount is under the condition of 10-20 head, and Amblyseius Orientalis Ehara and the plan genus amblyseius berlese that becomes mildewed is compared, and its female one-tenth mite becomes the day predation amount of mite to citrus red mite (crm)
[4,5]apparently higher than intending the day predation amount of genus amblyseius berlese to citrus red mite (crm) that become mildewed
[6], the former predation amount is 4-11 head, and the latter's predation amount is only 2-3 head.Supplying with under the condition of 20 Tetranychus urticae children mites, the predation amount of above-mentioned three kinds of Predatory Mites is similar, there is no significant difference, is 16-18 head.Because intend becoming mildewed, genus amblyseius berlese and the genus amblyseius berlese that becomes mildewed can only be raised with tetranychid at present, there is no simpler, cheap raising method, if so can realize, Amblyseius Orientalis Ehara is simple, cheap, a large amount of production efficiently, it will can replace expensive plan become mildewed genus amblyseius berlese and the genus amblyseius berlese that becomes mildewed completely in Control of Pest Mites, become the first-selected Predatory Mites of control tetranychid
Compare with amblyseius cucumeris oudeman (Amblyseiuscucumeris) with the Amblyseius barkeri of commercially producing (Amblyseius barkeri), the advantage that Amblyseius Orientalis Ehara has is:
(1) compare with Amblyseius barkeri, Amblyseius Orientalis Ehara is stronger to the prey ability of citrus red mite (crm), Tetranychus urticae, Tetranychus cinnabarinus.Gan Ming etc.
[5]the female one-tenth mite of Amblyseius Orientalis Ehara of report under 16-28 ℃ of condition to citrus red mite (crm) become mite day maximum predation amount apparently higher than Ling Peng etc.
[7]the female one-tenth mite of Amblyseius barkeri of report becomes the day maximum predation amount of mite under 16-28 ℃ of condition to citrus red mite (crm), the former day maximum predation amount is 14.6-28.8 head, and the latter's day maximum predation amount is only 5.78-9.71 head; We compare Amblyseius Orientalis Ehara and Amblyseius barkeri in laboratory, find that Amblyseius Orientalis Ehara is significantly higher than Amblyseius barkeri predation amount under equal conditions to the predation amount of above-mentioned several tetranychids.
(2) compare with amblyseius cucumeris oudeman, Amblyseius Orientalis Ehara is also very strong to the prey ability of citrus red mite (crm), Tetranychus urticae, Tetranychus cinnabarinus.Gan Ming etc.
[5]it is 6-13 head (when prey amount is 20-32) that the female one-tenth mite of Amblyseius Orientalis Ehara of report becomes a day predation amount for mite to citrus red mite (crm) under 24-28 ℃ of condition, apparently higher than Zhang Yanxuan etc.
[8]the amblyseius cucumeris oudeman of report is the day predation amount 4-5 head to citrus red mite (crm) under 25-26 ℃ of condition; We compare Amblyseius Orientalis Ehara and amblyseius cucumeris oudeman in laboratory, find that Amblyseius Orientalis Ehara is significantly higher than amblyseius cucumeris oudeman predation amount under equal conditions to the predation amount of above-mentioned several tetranychids.
In addition, the adaptability of Amblyseius Orientalis Ehara and colonization ability are stronger.Amblyseius Orientalis Ehara is this soil species of China, widely distributed in China, Jiu You Hebei, distribution province, Tianjin, Liaoning, Jiangsu, Anhui, Fujian, Jiangxi, Shandong, Hubei, Hunan, Guangdong and the Guizhou etc. now reported, and amblyseius cucumeris oudeman is from external introduction, adaptive capacity is poor, a little less than competitive ability.Field investigation not yet finds that Gai Man China grows surely, and the amblyseius cucumeris oudeman of release cannot find in field after producing season at one.
Therefore, compare Amblyseius barkeri and amblyseius cucumeris oudeman, Amblyseius Orientalis Ehara is to the prey ability of tetranychid is stronger, adaptability is wider, is more suitable for the control of tetranychid.
Yet although Amblyseius Orientalis Ehara has some above-mentioned advantages, China is sweet bright from calendar year 2001
[5]deng reporting " research of Amblyseius Orientalis Ehara to citrus red mite (crm) predation effect " 10 years so far later, remove Zheng Fangqiang etc.
[10]outer nobody carried out research report to Amblyseius Orientalis Ehara again, and also no one is applied to agricultural production by Amblyseius Orientalis Ehara, and more nobody utilizes its commercialization, and its basic reason is that a large-scale production difficult problem for the Cheap highly effective of Amblyseius Orientalis Ehara do not capture all the time.
The food of the raised Amblyseius Orientalis Ehara of having reported has pollen, tetranychid, honey, as sponge gourd pollen, India canna pollen, Ageratum conyzoides pollen, the numb pollen etc. of reviving, also have citrus red mite (crm), panonychus ulmi, tetranychus viennensis, cypress unguiculus mite (Oligonychusperditus) etc., and the combination food of castor-oil plant pollen and honey, citrus red mite (crm) and molasses water, in addition the combination food of pollen, two spotted spider mite, syrup.In these foods, pollen cost is high, difficult gathers, and raising complicated operation, the cost of tetranychid are high, energy consumption is large, efficiency is low, take up room large, and therefore existing raising method is unsuitable for the scale of Amblyseius Orientalis Ehara and commercially produces.
In finding new raising method, we are faced with the same predicament of forefathers, have also run into unprecedented problem.
First, in the literature, except the pollen of having reported, tetranychid, molasses water, do not find that Amblyseius Orientalis Ehara can prey on any report and the evidence of other prey, the existing unique authorized Predatory Mites of this point and China is raised patent and is compared very unfortunate.Authorized patent-" a kind of rearing method as Predatory Mites-amblyseius cucumeris oudeman ", this patent application is the relevant method of utilizing flour mite to raise amblyseius cucumeris oudeman, propose applied for flour mite in its original application file in, comprise tyrophagus putrescentiae (Tyrophagusputrescentiae), yet relevantly utilize the fact that tyrophagus putrescentiae can raise amblyseius cucumeris oudeman as far back as " functional response of Amblyseius cucumeris (Amblyseius cucumeris) to tyrophagus putrescentiae (Tyrophagusputrescentiae) ovum " of in June, 2000 report such as the Li Xinyi of Fudan University, October calendar year 2001, Fudan University just published in " Amblyseius cucumeris is the female functional response that becomes mite and Egg of Tyrophagus Putrescentiae to yellow tea mite " of the relation of temperature " Amblyseius cucumeris (Amblyseius cucumeris) development duration with " of report such as assistant qin etc. and the report such as the Li Xinyi of Fudan University in November calendar year 2001, can think that these three pieces of articles that Fudan University delivers have certain direct suggesting effect to this patent application.What is more, the documents and materials that just can utilize flour mite to raise relevant for Amblyseius cucumeris as far back as the eighties in last century abroad
[9].Yet but not any prompting of relevant Amblyseius Orientalis Ehara raising method.
Secondly, phytoseiid mite has the feature of kind diversity, feeding habits complexity, and not every Predatory Mites all available reserve substance mite class is raised.For example intend becoming mildewed genus amblyseius berlese, the genus amblyseius berlese that becomes mildewed, Chilean little phytoseiid mite (Phytoseiulus persimilis) only can be raised with tetranychid at present, can not raise by the reserve substance mite classes such as tyrophagus putrescentiae, oval baking soda mite (Aleuroglyphus ovatus) of raising amblyseius cucumeris oudeman.We attempt utilizing tyrophagus putrescentiae, oval baking soda mite to raise Amblyseius Orientalis Ehara, fail, and perhaps Amblyseius Orientalis Ehara is also the special feeding habits natural enemy of tetranychid as intend becoming mildewed genus amblyseius berlese, the genus amblyseius berlese that becomes mildewed, Chilean little phytoseiid mite, can only raise with tetranychid.
Finally, the feeding habits tool vicious understanding of forefathers to Amblyseius Orientalis Ehara, thinks that Amblyseius Orientalis Ehara is the Predatory Mites of specially eating tetranychid, can not take food other prey except tetranychid.Contained according to existing documents and materials, Amblyseius Orientalis Ehara is only preyed on tetranychid and as the biological control thing of tetranychid, therefore, it is habitually thought the special feeding habits natural enemy of tetranychid always, this viewpoint one piece of paper " quantitative analysis of Fuji apple orchard insect and Natural Enemies " that domestic one-level academic journal < < Chinese Journal of Applied Ecology > > delivered in 2009
[10]in also admitted, supported.Therefore, the wrong understanding of Amblyseius Orientalis Ehara feeding habits has further been limited again to our new exploration to the new raising method of the new prey of Amblyseius Orientalis Ehara.
The problem of mentioning based on above, can simple, cheap, the efficient raising method that realize Amblyseius Orientalis Ehara just unknown.
Summary of the invention
The new method that the object of this invention is to provide a kind of artificial mass rearing Amblyseius Orientalis Ehara; the method can be completely free of the existing problem such as high, the complicated operation of existing cost while utilizing tetranychid, pollen, sugar, honey etc. directly to raise Amblyseius Orientalis Ehara, can produce by large-scale breeding simpler, cheap, that realize efficiently Amblyseius Orientalis Ehara.
Technical scheme provided by the present invention is: under 16-35 ℃, relative moisture 60%-95% condition, utilize the method for the artificial mass rearing Amblyseius Orientalis Ehara of composition of hesperidium mite or hesperidium mite and other food.
Concrete operations are first to raise, prepare hesperidium mite, if use other prey in the feeding process of genus amblyseius berlese in the Orient, also to prepare in advance this kind of food, within containing raising medium or the raising container without raising medium, utilize again the composition of hesperidium mite or hesperidium mite and other food to raise Amblyseius Orientalis Ehara, wherein the initial access amount of Amblyseius Orientalis Ehara is thrown according to the expected volume of Amblyseius Orientalis Ehara, in feeding process, need monthly to add 1-6 hesperidium mite and breeding feed thereof, raise 15-45 days, during amount >=10 of Amblyseius Orientalis Ehara in breeding feed/gram, get final product packed products, expand numerous raising or cryopreservation, wherein:
(1) described raising hesperidium mite refers to and utilizes the feed of hesperidium mite to raise hesperidium mite, and wherein the feed of hesperidium mite can be that pollen, yeast, sugar, honey, royal jelly, molasses water etc. are multiple;
(2) described preparation hesperidium mite refers to the insect density according to hesperidium mite, is ready to enough hesperidium mites for raising Amblyseius Orientalis Ehara, and wherein the insect density of hesperidium mite is >=0.1/gram, but preferred 200-500 head/gram;
(3) described in, contain and raise medium or refer in the process of raising Amblyseius Orientalis Ehara, to have the medium of raising also can be without raising medium without raising medium, but preferably have raising medium and raising media water-bearing 5%-30%, raising container inner height, be 1-15 centimetre, preferred heights is 3-7 centimetre, wherein raise medium and refer to the medium of producing while raising Amblyseius Orientalis Ehara, such as wheat bran, vermiculite, perlite, straw crushed material, wood chip, rice hulls, coconut palm chaff, wheat bran, buckwheat shell, green gram spermoderm, blade etc., various media are separately, two or more mixes use;
(4) refer to can be for raising any device, equipment, the utensil of Amblyseius Orientalis Ehara for described raising container, such as the raising platform that utilizes the manufacture of water pond method to form, crisper, bucket etc., has good gas to exchange but raising container needs effectively to stop mite class to escape and ensure in container;
(5) composition of described hesperidium mite and other food refers in feeding process and can add other food, but in the feeding process of genus amblyseius berlese, at least used hesperidium mite in the Orient, wherein said other food refers to the food except hesperidium mite, such as the ovum of the mite class in reserve substance, insect and pollen, molasses water, tetranychid, yeast etc.;
(6) the described composition of hesperidium mite or hesperidium mite and other food that utilizes is raised the raising operating process that Amblyseius Orientalis Ehara refers to Amblyseius Orientalis Ehara, concrete operations are in hesperidium mite and Amblyseius Orientalis Ehara >=1: 1 ratio but preferably 10: 1-100: 1 ratio is added hesperidium mite and Amblyseius Orientalis Ehara to raise in container to, raise in container and also can add other food, wherein genus amblyseius berlese and hesperidium mite add in the process of raising container in the Orient, can add together with raising medium, breeding feed;
(7) described in, monthly add 1-6 hesperidium mite and breeding feed thereof and refer to every 5-30 days and add 1 hesperidium mite and breeding feed thereof, preferably monthly add 2-3 time;
(8) described packing refers to that Amblyseius Orientalis Ehara can use separately the composition of papery, irony, plastic material or multiple material to pack, and packaged form can be packed, bottled, canned etc.;
(9) described finished product refers to using Amblyseius Orientalis Ehara as a kind of product, and this product is the biological control that is applied to insect.
Adopt this method raising Amblyseius Orientalis Ehara to solve from Amblyseius Orientalis Ehara the difficulty of the raising since discovery (Ehara 1959) on the Plants in Japanese beech section, high, the necessary difficult problem that relies on tetranychid, pollen, molasses water raising of cost.
Meaning of the present invention is:
It is a kind of wrong understanding of special feeding habits Predatory Mites to Amblyseius Orientalis Ehara that the present invention has broken through forefathers, obtained unexpected technique effect, first passage practice confirms: Amblyseius Orientalis Ehara is not only the special feeding habits Predatory Mites of tetranychid, its except tetranychid, available hesperidium mite mass rearing also!
The present invention has realized simple, cheap, the efficient mass rearing of Amblyseius Orientalis Ehara, this brand-new raising scheme, in the Orient unprecedented precedent in the breeding technology history of genus amblyseius berlese!
The present invention has started new era for developing Amblyseius Orientalis Ehara!
Enforcement of the present invention, can also provide more, more efficient endemic species natural enemy for the control of the evil mites such as citrus red mite (crm), panonychus ulmi, tetranychus viennensis, for safety in production, the Environmental security of agriculture and forestry product are made positive contribution.
Embodiment
Following embodiment is only in order conveniently to understand the present invention, is not limitation of the present invention.
The method for optimizing of embodiment 1 artificial mass rearing Amblyseius Orientalis Ehara
At 20-30 ℃, under relative moisture 70%-90% condition, utilize separately hesperidium mite as raising the artificial mass rearing Amblyseius Orientalis Ehara of prey, concrete operations are first to raise, preparing insect density is the breeding feed of 200-500 head/gram fruit mite, within containing the raising container of raising medium, in hesperidium mite and the Amblyseius Orientalis Ehara ratio of 50: 1, add with raising the Amblyseius Orientalis Ehara of medium and with the hesperidium mite of breeding feed again, amount after the initial access breeding feed of Amblyseius Orientalis Ehara is 1/gram, 1 hesperidium mite of interpolation in every 10 days and breeding feed thereof in feeding process, feeding period is 30 days, the insect density of Amblyseius Orientalis Ehara in breeding feed gets final product >=20/gram, now Amblyseius Orientalis Ehara gets final product packed products, expand numerous raising or cryopreservation, can be with paper bag packing also available plastic bottle packing during packing, wherein:
Described raising media water-bearing 10%-20%, being highly 3-7 centimetre, is wheat bran and the vermiculite composition of 1: 1 by volume;
Described raising container refers to that floor space is that 0.1 square metre, height are the cylindrical salable crisper of 0.15 meter, and on crisper, there is the blow vent of one 0.01 square metres on top, gauze or paper sealing for blow vent.
Embodiment 2 utilizes hesperidium mite, application water pond method to raise the method for Amblyseius Orientalis Ehara
In the crisper of high 10 centimetres, wide 10 centimetres, long 10 centimetres, put into the high sponge that is respectively 8 centimetres, 8 centimetres, 5 centimetres of length and width, add again water in crisper, sponge keeps water high after fully absorbing water be 4 centimetres, then on sponge, put a circular filter paper, filter paper completely on sponge, then is put a circular black plastic film paper to filter paper central authorities, and black plastic film paper is slightly less than filter paper.On black plastic film paper, access 10 female one-tenth mites of Amblyseius Orientalis Ehara and 100-200 hesperidium mite, put again 1-2 sheet and eat female raw cook, crisper is placed under 27 ℃, the condition of relative moisture 75%, within every 7 days, add the female raw cook of 1 secondary eclipse, hesperidium mite, note adding the isolated water in crisper, can realize the raising of Amblyseius Orientalis Ehara and expand numerous.
Embodiment 3 utilizes the method for optimizing of the artificial mass rearing Amblyseius Orientalis Ehara of the composition of hesperidium mite and other food
At 16-35 ℃, under relative moisture 60%-95% condition, first raise, prepare hesperidium mite and prepare other prey, within containing the raising container of raising medium, in hesperidium mite and the Amblyseius Orientalis Ehara ratio of 50: 1, add with raising the Amblyseius Orientalis Ehara of medium and with the hesperidium mite of breeding feed again, amount after the initial access breeding feed of Amblyseius Orientalis Ehara is 1/gram, 1 hesperidium mite of interpolation in every 10 days and breeding feed thereof in feeding process, feeding period is 30 days, wherein utilizing hesperidium mite raising Amblyseius Orientalis Ehara in container, to throw in other mentioned prey again after 15 days, during amount >=10 of Amblyseius Orientalis Ehara in feed/gram, get final product packed products, expand numerous raising or cryopreservation, wherein:
Described raising medium is wheat bran and the vermiculite composition of 1: 1 by volume, and raising media water-bearing 5%-30%, the height in raising container are 3-7 centimetre;
Described raising container refers to can be for raising any device, equipment, the utensil of Amblyseius Orientalis Ehara, such as the raising platform that utilizes the manufacture of water pond method to form, crisper, bucket etc., needs effectively to stop mite class to escape and guarantees that in raising container, having good gas exchanges but raise container;
Described other food refers to the food except hesperidium mite, such as the ovum of the mite class in reserve substance, insect and pollen, molasses water, tetranychid, yeast etc.;
Described packing refers to that Amblyseius Orientalis Ehara can use separately the composition of papery, irony, plastic material or multiple material to pack, and packaged form can be packed, bottled, canned etc.
The impact that 1 hesperidium mite grows on Amblyseius Orientalis Ehara is implemented in test
Test method:
Test is carried out in 27 ℃, relative moisture 75%, complete dark incubator, in the ovum access test cell that Amblyseius Orientalis Ehara in 24 hours is produced, repeat 13 times, test cell is put in the crisper that contains moistening sponge, crisper is put in incubator again, if Amblyseius Orientalis Ehara becomes by ovum the random worm state of hesperidium mite that starts to feed after mite, until Amblyseius Orientalis Ehara is developed to into mite.
Result:
(1) impact that hesperidium mite grows on the female mite of Amblyseius Orientalis Ehara the results are shown in Table 1
As shown in Table 1, while feeding hesperidium mite, Amblyseius Orientalis Ehara ovum is only 6.6 ± 0.70 days to the phase of going through of female one-tenth mite, the hesperidium mite of feeding, and the female mite of Amblyseius Orientalis Ehara can complete and grow.
(2) impact that hesperidium mite grows on the male mite of Amblyseius Orientalis Ehara the results are shown in Table 2
As shown in Table 2, the hesperidium mite of feeding, the normal development of Amblyseius Orientalis Ehara ovum energy becomes mite to male, development duration is 6.3 ± 0.58 days, be slightly shorter than be developed to female one-tenth mite go through the phase.
(3) survival rate of Amblyseius Orientalis Ehara while feeding hesperidium mite
In 13 times that test repeats, while feeding hesperidium mite, the survival rate of Amblyseius Orientalis Ehara is 100%.
2 impacts of hesperidium mite on Amblyseius Orientalis Ehara egg laying amount are implemented in test
Test method:
Test is carried out in 27 ℃, relative moisture 75%, complete dark incubator, by raising with in hesperidium mite, the Amblyseius Orientalis Ehara access test cell of laying eggs, continues to raise with hesperidium mite, repeats 8 times.Test cell is put in the crisper that contains moistening sponge, and crisper is put in incubator again, and Continuous Observation 3 days, records egg laying amount.
The results are shown in Table 3.
From table 3, Amblyseius Orientalis Ehara takes food hesperidium mite and can lay eggs, and every per day egg laying amount is 1.29 ± 0.21.
The impact that table 1 hesperidium mite grows on the female mite of Amblyseius Orientalis Ehara (my god)
The impact that table 2 hesperidium mite grows on the male mite of Amblyseius Orientalis Ehara (my god)
The egg laying amount of Amblyseius Orientalis Ehara (grain) when table 3 takes food hesperidium mite
The impact of 3 tyrophagus putrescentiaes on Amblyseius Orientalis Ehara egg laying amount implemented in test
Test method:
Test is carried out in 27 ℃, relative moisture 75%, complete dark incubator, to raise with in hesperidium mite, the Amblyseius Orientalis Ehara access test cell of laying eggs, hungry 24 hours, choose lay eggs, for continuing, the random worm state of tyrophagus putrescentiae is raised, and repeats 10 times, test cell is put in the crisper that contains moistening sponge, crisper is put in incubator again, and Continuous Observation 8 days, records egg laying amount.
The results are shown in Table 4.
From table 4, while utilizing tyrophagus putrescentiae to feed Amblyseius Orientalis Ehara, Amblyseius Orientalis Ehara only had the phenomenon of laying eggs at the 2nd day, did not find afterwards to lay eggs always, fed after 9 days, and Amblyseius Orientalis Ehara lethality is up to 40%.Illustrate that to utilize the tyrophagus putrescentiae Amblyseius Orientalis Ehara feeding effect of feeding bad, and wherein the 2nd day have a phenomenon of laying eggs, that Amblyseius Orientalis Ehara has the situation of predation tyrophagus putrescentiae after hungry 24 hours most probably, supplemented portion of energy, impel the established ovum output of script external, but can not maintain Amblyseius Orientalis Ehara, continue output.This result is compared with utilizing the feed egg laying amount of Amblyseius Orientalis Ehara of hesperidium mite, and significant difference is very large, thereby also embody, utilizes the hesperidium mite Amblyseius Orientalis Ehara successful of feeding very good, poor effect while raising Amblyseius Orientalis Ehara with tyrophagus putrescentiae.
The egg laying amount of Amblyseius Orientalis Ehara when table 4 is raised with tyrophagus putrescentiae
Note: in table, D represents that Amblyseius Orientalis Ehara is dead.
List of references:
[1] Zhu Zhimin, Lai Yongfang. Chinese research with utilize Predatory Mites overview, spider shape journal, 1992.
[2] Yang Ziqi, Cao Kejia, Li Weiping. Amblyseius Orientalis Ehara First Report of Studies, Natural Enemies of Insects, 1987.
[3] Zhang Shouyou, Cao Xinwen, Han Zhiqiang, Wu Weinan. Amblyseius Orientalis Ehara is to the tetranychid Natural control action research of two kinds, apple orchard, Natural Enemies of Insects, 1992.
[4] Lai Yongfang, Zhu Zhimin. the predation of Amblyseius Orientalis Ehara to citrus red mite (crm) and cypress unguiculus mite, Jiangxi plant protection, 1991.
[5] sweet bright, Li Minghui, Hu Siqin. the research of Amblyseius Orientalis Ehara to citrus red mite (crm) predation effect, University Of Nanchang's journal, 2001.
[6] Zou Ping, Gao Jianrong, Ma Enpei. intend becoming mildewed genus amblyseius berlese predatism and predation quantity research, Natural Enemies of Insects, 1986.
[7] Ling Peng, Xia Bin, Li Pengxin, happy, Zhong Ling, Li Aihua. the predation usefulness of Amblyseius barkeri to citrus red mite (crm), spider shape journal, 2008.
[8] Zhang Yanxuan, woods is faithful, Ji Jie, Chen Chuanpei, Lin Zhang. and amblyseius cucumeris oudeman is controlled oranges and tangerines harmful mite study progress, Insects in China association Annual Conference in 2002,2002.
[9]Ramarkers，1983.Mass?production?and?introduction?of?Amblyseius?mckenziei?and?A.cucumeris.SROP/WPRS?Bull.6(3)，203-206.
[10] Zheng Fangqiang, Zhang Xiaohua, Qu Chenghuai, Liu Xueqian, Qu Shujuan. the quantitative analysis of Fuji apple orchard insect and Natural Enemies, Chinese Journal of Applied Ecology, 2009.
Claims (2)
1. the method for an artificial mass rearing Amblyseius Orientalis Ehara, it is characterized in that at 16-35 ℃, under relative moisture 60%-95% condition, utilize the artificial mass rearing Amblyseius Orientalis Ehara of composition of hesperidium mite or hesperidium mite and other food, concrete operations are first to raise, prepare hesperidium mite, within containing raising medium or the raising container without raising medium, utilize again the composition of hesperidium mite or hesperidium mite and other food to raise Amblyseius Orientalis Ehara, wherein the initial access amount of Amblyseius Orientalis Ehara is thrown according to the expected volume of Amblyseius Orientalis Ehara, in feeding process, need monthly to add 1-6 hesperidium mite and breeding feed thereof, raise 15-45 days, during amount >=10 of Amblyseius Orientalis Ehara in breeding feed/gram, get final product packed products, expand numerous raising or cryopreservation, described preparation hesperidium mite refers to the insect density according to hesperidium mite, be ready to enough hesperidium mites for raising Amblyseius Orientalis Ehara, wherein the insect density of hesperidium mite is >=0.1/gram, adding proportion >=1 of hesperidium mite and Amblyseius Orientalis Ehara: 1.
2. the method for artificial mass rearing Amblyseius Orientalis Ehara according to claim 1, is characterized in that described other food refers to mite class, tetranychid, the ovum of insect, pollen, sugar, honey, the yeast in reserve substance.
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| CN103563855A (en) * | 2013-05-20 | 2014-02-12 | 中国农业科学院植物保护研究所 | New method for feeding prey mites and predatory mites |
| CN103651270A (en) * | 2013-12-03 | 2014-03-26 | 四川农业大学 | Breeding method of small new neoseiulus californicus |
| CN103750016B (en) * | 2013-12-26 | 2015-11-25 | 中山大学 | A kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof |
| CN105724321A (en) * | 2016-03-09 | 2016-07-06 | 中国农业科学院植物保护研究所 | Amblyseius tsugawai breeding method and application of amblyseius tsugawai |
| CN105794725B (en) * | 2016-03-24 | 2018-11-27 | 中国农业科学院植物保护研究所 | Predatory Mites and its method for breeding of prey mite |
| CN109699578A (en) * | 2017-10-25 | 2019-05-03 | 石河子大学 | The method for raising the new small peaceful mite of double tails using prey flour mite |
| IL257892A (en) * | 2018-03-05 | 2018-05-02 | Bio Bee Sde Eliyahu Ltd | Novel methods for rearing and controlled release of biological control agents |
| CN112753661B (en) * | 2021-02-01 | 2022-01-11 | 内蒙古农业大学 | Application of neoseiulus striatus in prevention and control of small sap-sucking pests and propagation method thereof |
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| CN101049098A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Culture medium for preserving species of mites |
| CN101965822B (en) * | 2010-10-29 | 2015-02-11 | 中国农业科学院植物保护研究所 | Method for artificially breeding mass Carpoglyphus lactis |
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