CN103651270A - Breeding method of small new neoseiulus californicus - Google Patents
Breeding method of small new neoseiulus californicus Download PDFInfo
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- CN103651270A CN103651270A CN201310671751.1A CN201310671751A CN103651270A CN 103651270 A CN103651270 A CN 103651270A CN 201310671751 A CN201310671751 A CN 201310671751A CN 103651270 A CN103651270 A CN 103651270A
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Abstract
The invention discloses a breeding method of small new neoseiulus californicus, and belongs to the field of bioengineering. The method comprises the steps that mature sword bean leaves are selected, cut to be of a preset graph and placed into a culture dish with wet cotton laid at the bottom; eggs of neoseiulus californicus are added, eggs, generated within a first preset period, of the small new neoseiulus californicus are placed on the sword bean leaves, and apple pollen is thrown into the culture dish; the culture dish is sealed through a preservative film, and the pollen is replaced according to the first preset period. By breeding of more than ten kinds of pollen, the apple pollen is screened out as the excellent goal pollen, the edible period of the pollen is prolonged by improving the breeding environment, and waste caused when the pollen goes bad is reduced. The small new neoseiulus californicus is bred through the apple pollen, can be bred in a transgenerational mode, and can normally grow, develop, mate and lay eggs, and laid eggs can smoothly hatch.
Description
Technical field
The present invention relates to bioengineering field, particularly the new little peaceful mite raising method in a kind of California.
Background technology
The new little peaceful mite in California can prey on the multiple harmful mites such as Tetranychus cinnabarinus, oranges and tangerines beginning tetranychid, panonychus citri, and Using predatory mite to control pest mites is significant for reducing residue of pesticide and green agriculture.
Predatory Mites carry out that biological control uses abroad relatively extensively and technology also comparatively ripe.China is being also in the primary stage aspect application Predatory Mites control evil mite, also more limited in the area of application.Comparatively ripe kind has amblyseius cucumeris oudeman and Amblyseius barkeri, but at Sichuan province amblyseius cucumeris oudeman owing to not surviving the winter, need to manually discharge every year, expenses for prevention and control increases, waste time and energy.And the new little peaceful mite in California has the certain control ability of evil mite, and suitable to Sichuan Overwintering.
In general breeding of Predatory Mites have natural prey method for breeding, substitute prey method for breeding, pollen method for breeding, four kinds of approach of artificial feed method for breeding.Natural prey method for breeding ratio is easier to successfully, but but scale breeding time can run into many technical barriers, the reproductive process of natural prey is generally the cultivation of natural prey host plant, inoculates natural prey, a plurality of links such as inoculation Predatory Mites, separated packing, storage transportation.Wherein separated Predatory Mites need to specifically be washed mite machine, and due to business, technique is maintained secrecy, and is difficult to obtain.While throwing in Predatory Mites, remaining harmful mite harm prevention target is also a very large risk.And substitute prey, be applicable to the wider Predatory Mites of recipe, and the new little peaceful mite in California does not belong to this.
The new little peaceful mite in California belongs to Phytoseiidae, and phytoseiid mite can utilize pollen as transition food conventionally.But not that each pollen is all applicable to Predatory Mites and takes food.We turn to three levels by the suitability of pollen, and worst one-level is that Predatory Mites will not be searched for food.The second is to take food, but can not complete successive propagation, can not mate and oviposit.The third is to help Predatory Mites to complete whole Development History, can mate and oviposit, and ovum can be hatched smoothly.
Through screening, existing Apple Pollen effect is more satisfactory, but there are the following problems: 1. the rotten problem of pollen.When raising the new little peaceful mite in California with pollen, need surround circle with wet cotton bar, to prevent and treat it and escape, pollen easily makes moist and becomes sticky thickly, or while changing pollen, airborne spore intrusion pollen, goes mouldy it, is all unfavorable for taking food.2. the problem that the new little peaceful mite in California escapes.Raise after a period of time, the new female mite of little peaceful mite in the California in egg-laying period can climb wet cotton bar.3. the collection of pollen and seasonal problem.Specific pollen has certain acquisition time restriction, and need to expend suitable time energy.The Apple Pollen using is at present the pollination pollen of buying, some costliness of price, and also the consumability period of pollen is shorter.
Summary of the invention
In order to solve the California required pollen of new little peaceful mite in prior art, go bad and the shorter problem of consumability period.For the as above problem running in prior art, the present invention proposes following technical scheme:
The new little peaceful mite raising method in California, described method comprises the steps:
Choose ripe sword bean blade, be cut into default figure, and put into the culture dish that cotton is laid in bottom, described cotton is moistening;
The ovum of the new little peaceful mite in access California, is placed in the ovum of the new little peaceful mite producing in the first predetermined period on described sword bean blade, and drops into Apple Pollen;
Described culture dish is sealed with preservative film, and follow and change pollen by described the first predetermined period.
The present invention raises California genus amblyseius berlese by planting pollen with more than ten, and statistics indices, filters out good object pollen---Apple Pollen, and by improving feeding environment, extends pollen consumability period, reduces the waste producing because of rotten.While raising the new little peaceful mite in California, can successive propagation, can normal growth growth, mating, lay eggs, the ovum producing can be hatched smoothly.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The embodiment of the present invention provides a kind of California new little peaceful mite raising method, and the method comprises the steps:
1, choose ripe sword bean blade, be cut into default figure, and put into the culture dish that cotton is laid in bottom, this cotton is moistening;
In an embodiment of the present invention, cotton water is moistening, and is close to moistening cotton bar around at sword bean blade, to prevent that the new little peaceful mite in California from escaping.
As a preferred embodiment of the present invention, default figure is circular.
The diameter of culture dish is 9cm, bottom pad one deck cotton, and water is moistening, but can not ponding too much, keeps moistening.Choose ripe thick sword bean blade and be cut into circle, be close to wet cotton, both can delay blade withered, wettable environment can be used as the barrier that stops Predatory Mites to be escaped again.Again cotton bar is closely trapped among around circular sword bean leaf, moistening, do not stay gap, but can not make water droplet enter blade.
2, the ovum of the new little peaceful mite in access California, is placed in the ovum of the new little peaceful mite producing in the first predetermined period on sword bean blade, and drops into Apple Pollen;
In an embodiment of the present invention, the first predetermined period is 24 hours.
3, culture dish is sealed with preservative film, and follow and change pollen by the first predetermined period.
In an embodiment of the present invention, on preservative film, be with porose.
The new ovum of little peaceful mite in the institute California of producing, is placed in circular blade in picking some 24h, drops into a small amount of Apple Pollen, with preservative film, culture dish is sealed, and pricks several apertures, for taking a breath on preservative film.Every 12h observes the developmental state of a Predatory Mites, and every 24h changes a pollen.
Concrete feeding process is as follows:
Plantation sword bean, as the food source of the natural prey Tetranychus cinnabarinus of California genus amblyseius berlese, this is because sword bean leaf breeding Tetranychus cinnabarinus is rapid, and sword bean is easy to plantation, simple and convenient management.Choose aging thicker blade, clean and dry, put into and raise box, access Tetranychus cinnabarinus, is prepared against upward in the back side.Under sword bean leaf, need to spread paper or the cotton that water absorbing force is strong, often add water and keep paper or cotton moistening, can prevent acarid escape, maintain the required air humidity of acarid and wet environment, delay the withered of sword bean leaf.With stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp its population and change.After access Tetranychus cinnabarinus, to note controlling population quantity, too much can cause sword bean leaf to consume too fast.Can regulate the population of Tetranychus cinnabarinus to change by changing temperature, Tetranychus cinnabarinus be in preference temperature, and the higher growth and breeding of temperature is faster, suitably reduces temperature and can delay its growth rate.When Tetranychus cinnabarinus population reaches some, with small size writing brush access California genus amblyseius berlese, the access quantity of California genus amblyseius berlese can not be too much, otherwise excessively predation causes Tetranychus cinnabarinus population to decline rapidly, and raising is by unsustainable.
The embodiment of the present invention is raised California genus amblyseius berlese by planting pollen with more than ten, and statistics indices, filters out good object pollen---Apple Pollen, and by improving feeding environment, extends pollen consumability period, reduces the waste producing because of rotten.While raising the new little peaceful mite in California, can successive propagation, can normal growth growth, mating, lay eggs, the ovum producing can be hatched smoothly.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (5)
1. the new little peaceful mite raising method in California, is characterized in that, described method comprises the steps:
Choose ripe sword bean blade, be cut into default figure, and put into the culture dish that cotton is laid in bottom, described cotton is moistening;
The ovum of access California genus amblyseius berlese, is placed in the ovum of the new little peaceful mite in the described California producing in the first predetermined period on described sword bean blade, and drops into Apple Pollen;
Described culture dish is sealed with preservative film, and on bundle, some apertures are used as to breathe freely, and follow and change pollen by described the first predetermined period.
2. the method for claim 1, is characterized in that, described cotton water is moistening, and is close to the described water-moistened cotton of using around at sword bean blade.
3. the method for claim 1, is characterized in that, described default figure is circular.
4. the method for claim 1, is characterized in that, described the first predetermined period is 24 hours.
5. the method for claim 1, is characterized in that, is with porose on described preservative film.
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| CN201310671751.1A CN103651270A (en) | 2013-12-03 | 2013-12-03 | Breeding method of small new neoseiulus californicus |
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| CN201310671751.1A CN103651270A (en) | 2013-12-03 | 2013-12-03 | Breeding method of small new neoseiulus californicus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858828A (en) * | 2014-03-27 | 2014-06-18 | 李庆 | Method for breeding neoseiulus californicus through apricot blossom pollen |
| CN104430194A (en) * | 2014-11-14 | 2015-03-25 | 中国农业科学院柑桔研究所 | Method for artificially feeding euseius nicholsi in pollen room |
| CN104472437A (en) * | 2014-11-20 | 2015-04-01 | 中国农业科学院柑桔研究所 | Indoor isolation feeder for spider mites |
| CN105660539A (en) * | 2016-01-26 | 2016-06-15 | 贵阳学院 | Method for reproducing stegobium paniceum (L.) by utilizing semi-artificial feed |
| CN107466972A (en) * | 2017-09-19 | 2017-12-15 | 南昌大学 | A kind of conservation method for breeding of Tetranychus cinnabarinus |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858828A (en) * | 2014-03-27 | 2014-06-18 | 李庆 | Method for breeding neoseiulus californicus through apricot blossom pollen |
| CN104430194A (en) * | 2014-11-14 | 2015-03-25 | 中国农业科学院柑桔研究所 | Method for artificially feeding euseius nicholsi in pollen room |
| CN104472437A (en) * | 2014-11-20 | 2015-04-01 | 中国农业科学院柑桔研究所 | Indoor isolation feeder for spider mites |
| CN105660539A (en) * | 2016-01-26 | 2016-06-15 | 贵阳学院 | Method for reproducing stegobium paniceum (L.) by utilizing semi-artificial feed |
| CN105660539B (en) * | 2016-01-26 | 2018-06-15 | 贵阳学院 | Utilize the method for meridic diet procreation Stegobium paniceum |
| CN107466972A (en) * | 2017-09-19 | 2017-12-15 | 南昌大学 | A kind of conservation method for breeding of Tetranychus cinnabarinus |
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Application publication date: 20140326 |