CN104430194A - Method for artificially feeding euseius nicholsi in pollen room - Google Patents

Method for artificially feeding euseius nicholsi in pollen room Download PDF

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Publication number
CN104430194A
CN104430194A CN201410647940.XA CN201410647940A CN104430194A CN 104430194 A CN104430194 A CN 104430194A CN 201410647940 A CN201410647940 A CN 201410647940A CN 104430194 A CN104430194 A CN 104430194A
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China
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pollen
nissl
mite
raises
peaceful
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CN201410647940.XA
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Inventor
李鸿筠
王邦祥
冉春
刘浩强
胡军华
姚廷山
丛林
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CITRUS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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CITRUS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Priority to CN201410647940.XA priority Critical patent/CN104430194A/en
Publication of CN104430194A publication Critical patent/CN104430194A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Environmental Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Insects & Arthropods (AREA)
  • Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a method for artificially feeding euseius nicholsi in a pollen room. The method comprises the following steps that a, collected pollen is refrigerated and stored after being dried in the air; b, the euseius nicholsi is placed on a feeding table, on which the pollen is placed, in the feeding room, the temperature in the feeding room is kept at 20 DEG C to 25 DEG C, the relative humidity is 70%-85%, the illumination time ranges from 8 hours to 12 hours, the illumination intensity is 600-800 lux, and an egg laying cluster used for collecting eggs is placed on the feeding table; c, the collected eggs are placed on another feeding table on which the pollen is placed, hatched, fed and collected. The method has the advantages that food sources are convenient to obtain, durable, safe and reliable, multiple generations can be fed continuously, reproduction of the nicholsi is not influenced, the nicholsi is the same as that growing under the natural condition and can be effectively prevented from escaping during the cultivation period, and the survival rate is high.

Description

Pollen indoors artificial raises the method for the very peaceful mite of Nissl
Technical field
The present invention relates to field of agricultural sciences, particularly a kind of pollen indoors artificial raises the method for the very peaceful mite of Nissl.
Background technology
The very peaceful mite of Nissl is widely distributed in China, have a kind of Important Natural Enemy of stronger Natural control action to evil mite, can prey on citrus red mite (crm), the citrus beginning tetranychid, Tetranychus urticae, Tetranychus cinnabarinus, Polyphagotarsonemus latus Banks, panonychus ulmi, the multiple harmful mite such as pink tea rust mite.Nissl really pacifies mite when being applied as natural enemy by artificial method, to citrus beginning Panonychus citri, the citrus beginning tetranychid, Tetranychus cinnabarinus, the evil mite such as Tetranychus urticae have good control effects.Because this mite development duration is short, reproduction speed is fast, and the female one-tenth mite life-span is long, and predation scope is wide, heavy, carries out biological control, can obviously reduce Pesticide use amount, reduce the persticide residue in agricultural product, improve agricultural output and quality with it to evil mite.Using the Nissl of artificial propagation very peaceful acarid control evil mite, is the biological control method to eco-friendly, and, organic farm products especially green in agricultural production has broad application prospects in producing.Application number is 201210232815.3 rearing methods disclosing the very peaceful mite of a kind of Nissl, adopt the culturing room that three mutually isolated, cultivate Tetranychus urticae respectively, the very peaceful mite of Kidney bean and Nissl, using Kidney bean as the food of Tetranychus urticae, food again using Tetranychus urticae as the very peaceful mite of Nissl, the complex steps of cultivating, and food source is inconvenient, can not ensure that cultivated food is natural pollution-free disease-free, therefore the safety of the food of the very peaceful mite of Nissl cannot be ensured, and adopt different food to cultivate required nutrition and quality in the very peaceful whole growth cycle of mite of very difficult guarantee Nissl, and the problem of the very peaceful mite escape of Nissl cannot be solved, survival rate is undesirable, because food source host plant cultivated by needs, the cultivation of plant can climate season etc. factor impact, many cultures cannot be carried out by continuous print, and needs need the migration through three culturing room in the nurturing period of mite, not only complex operation, also can affect to mite.
Therefore, need the method for the very peaceful mite of a kind of new artificial feeding Nissl, having food source facilitates lasting security reliable, many generations can be carried out continuously raise and do not affect the reproduction of mite, can be the same with the growth under natural conditions, and effectively can prevent the escape problem of mite in the training period, and the characteristic that survival rate is high.
Summary of the invention
In view of this, a kind of pollen indoors artificial is the object of the present invention is to provide to raise the method for the very peaceful mite of Nissl, having food source facilitates lasting security reliable, many generations can be carried out continuously raise and do not affect the reproduction of mite, can be the same with the growth under natural conditions, and effectively can prevent the escape problem of mite in the training period, and the characteristic that survival rate is high.
Pollen indoors artificial of the present invention raises the method for the very peaceful mite of Nissl, comprises the following steps:
A. the pollen of collection is dried rear freezing;
B. mite of really being pacified by Nissl is placed on the polliniferous raising platform of placement of receptacle, receptacle temperature is kept to be 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8-12 hour, intensity of illumination 600-800 lux, and platform places for collecting laying eggs bunch of ovum raising;
C. the ovum of collection is positioned over after another to place on polliniferous raising platform hatching and raises and adopt ovum.
Further, in step a, described pollen is robur pollen, dries in the shade or dry after the robur florescence gathers micro-dew yellow-white flower fringe, puts into the refrigerator storage that temperature is less than-4 DEG C after then loading test tube;
Further, described raising platform is polyurethane foam and the black cloth that fully water suction put into by porcelain dish after sterilization or culture dish, the smooth scraps of paper are put repave last layer transparent plastic film after boiling water shower after, then evenly tremble the pollen of preservation on the scraps of paper, and surround the water segregation raising platform of surrounding formation with moistening cotton thread;
Further, lay eggs bunch for some cotton silks or bar colligation are made described in;
Further, within 2 days ~ 3 days, 1 pollen is changed;
Further, described moist closet body of wall insulation material, glass two-layer warm-keeping glass, and avoid direct sunlight, first whitewashes with lime water before raising, then uses 3% formalin spraying disinfection, under 25 DEG C of conditions airtight more than 24 hours;
Further, receptacle temperature is kept to be 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8 hours, intensity of illumination 667 lux;
Further, described Nissl is really pacified, and to perch plant leaf blade be one or both in Japan, purple perilla to mite, gathers the more Nissl such as Japan, purple perilla in worm source mite of really pacifying and perch plant leaf blade, with No. 0 each worm state of writing brush picking, put down gently on water segregation raising platform from field;
Further, also comprise rejuvenation: gather the very peaceful mite of a certain amount of Nissl every year to field and become mite, raise together with the mite of indoor propagation, thus population is maintained vigour.
Beneficial effect of the present invention: pollen indoors artificial of the present invention raises the method for the very peaceful mite of Nissl, utilize pollen as the food source of the very peaceful mite of Nissl, material is easy to get, supply of long duration can be met, safe and reliable, many generations can be carried out continuously and raise and do not affect the reproduction of mite, can be the same with the growth under natural conditions, and effectively can prevent the escape problem of mite in the training period, and the characteristic that survival rate is high.
Embodiment
Pollen indoors artificial of the present invention raises the method for the very peaceful mite of Nissl, comprises the following steps:
A. the pollen of collection is dried rear freezing; Be convenient to the long-term preservation of food and never degenerate, nutrient component does not run off.
B. mite of really being pacified by Nissl is placed on the polliniferous raising platform of placement of receptacle, receptacle temperature is kept to be 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8-12 hour, intensity of illumination 600-800 lux, and platform places for collecting laying eggs bunch of ovum raising; To lay eggs bunch for mesh-like is convenient to collect.
C. the ovum of collection is positioned over after another to place on polliniferous raising platform hatching and raises and adopt ovum.Be convenient to breed continuously.
In the present embodiment, in step a, described pollen is robur pollen, dries in the shade or dry after the robur florescence gathers micro-dew yellow-white flower fringe, puts into the refrigerator storage that temperature is less than-4 DEG C after then loading test tube.
In the present embodiment, described raising platform is polyurethane foam and the black cloth that fully water suction put into by porcelain dish after sterilization or culture dish, the smooth scraps of paper are put repave last layer transparent plastic film after boiling water shower after, then put the pollen of preservation, and surround the water segregation raising platform of surrounding formation with moistening cotton thread; Avoid the very peaceful mite of Nissl to escape, and be beneficial to the certain humidity of maintenance, improve survival rate.
In the present embodiment, described in lay eggs bunch for some cotton silks or bar colligation being made cotton bunch (get one little cotton, scoop up with a hand, cotton bunch).
In the present embodiment, within 2 days ~ 3 days, change 1 pollen.
In the present embodiment, described moist closet body of wall insulation material, glass two-layer warm-keeping glass, and avoid direct sunlight, first whitewashes with lime water before raising, then uses 3% formalin spraying disinfection, under 25 DEG C of conditions airtight more than 24 hours.
In the present embodiment, receptacle temperature is kept to be 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8 hours, intensity of illumination 667 lux.
In the present embodiment, described Nissl is really pacified, and to perch plant leaf blade be one or both in Japan, purple perilla to mite, gather the very peaceful mite of the more Nissl such as Japan, purple perilla in worm source from field and perch plant leaf blade, by No. 0 each worm state of writing brush picking, put down gently and raise on platform at water segregation.
In the present embodiment, also comprise rejuvenation: gather the very peaceful mite of a certain amount of Nissl every year to field and become mite, raise together with the mite of indoor propagation, thus population is maintained vigour.
Below by specific embodiment, the present invention is further elaborated.
1 feed
Robur pollen.
1.1 pollen collection
Start mid-March to observe robur flowering time, once bloom, namely adopt lap waste fringe.Coming into bloom, gathering micro-dew yellow-white flower fringe, turn white, yellow person then abandons it.
1.2 pollen airings
Be placed on after Hua Sui adopts back in bamboo sieve or other ventilation object object, thin layer airing, dries in the shade or dries, pollen covers gauze, both avoiding direct sunlight, avoids again pollen to be blown away.
1.3 pollen storage
Pollen dries rear loading test tube, mouth of pipe plug tampon, puts into-4 DEG C of refrigerator storages.
2 raise
2.1 live boxs need condition
Receptacle needs well-ventilated, drying, health, avoids direct sunlight, air can be kept fresh, without poisoning, water damage, the plague of rats, cockroach etc.Body of wall insulation material, glass two-layer warm-keeping glass.Sweep before raising, clear up, must not foreign material be stacked, first whitewash with lime water, then use 3% formalin spraying disinfection, under 25 DEG C of conditions airtight 24 hours.Breeding facility 75% alcohol disinfecting.Receptacle needs personal management, regulates temperature, humidity, and note doing sanitation and hygiene well, timing keeps a record.
Raise temperature required 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8 hours, intensity of illumination 667 lux.
2.2 raise equipment
By putting into polyurethane foam and the black cloth of fully water suction with the porcelain dish after sterilization or culture dish, drench one time with boiling water, repave last layer transparent plastic film, and surround surrounding with moistening cotton thread, form water segregation and raise platform.
2.3 worm sources
Gather the very peaceful mite of the more Nissl such as Japan, purple perilla in worm source from field and perch plant leaf blade, take back each worm state of indoor No. 0 writing brush picking, put down gently and raise on platform at water segregation.
2.4 raise
Put laying eggs bunch of the happy shape (making brush handle shape structure by cotton) be twisted into cotton raising platform surrounding, to collect ovum, and in dish, put several the smooth scraps of paper, it is trembled pollen as feed.Within 2 days ~ 3 days, change 1 pollen, and bundle of laying eggs is collected in the raising platform of brand-new, treat that it is hatched, give feed, make its breeding of growing.Continue to adopt ovum.
Collect before 2.5 releases
Discharge first 5 days ~ 7 days, with No. 0 each movable mite of writing brush picking about 300, (because mite flocks together, and writing brush end is soft can not damaging mite, tens of mites once can be chosen to obtain) with the writing brush end of happy shape, move in paper packaging bags, powder of inside letting off fireworks, bag mouth sealing.Collect ovum to be undertaken by 2.4 methods, put plastics into and support in scolite, often pipe 500 ~ 600.
2.6 conservation
Winter, conservation can be carried out in the insectary that insulation material makes body of wall, two-layer warm-keeping glass, left the very peaceful mite conservation of a small amount of Nissl and survived the winter.
2.7 rejuvenation
Gather the very peaceful mite of a certain amount of Nissl every year to field and become mite, raise together with the mite of indoor propagation, thus population is maintained vigour.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.

Claims (9)

1. pollen indoors artificial raises a method for the very peaceful mite of Nissl, it is characterized in that: comprise the following steps:
A. the pollen of collection is dried rear freezing;
B. mite of really being pacified by Nissl is placed on the polliniferous raising platform of placement of receptacle, receptacle temperature is kept to be 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8-12 hour, intensity of illumination 600-800 lux, and platform places for collecting laying eggs bunch of ovum raising;
C. the ovum of collection is positioned over after another to place on polliniferous raising platform hatching and raises and adopt ovum.
2. pollen indoors artificial according to claim 1 raises the method for the very peaceful mite of Nissl, it is characterized in that: in step a, described pollen is robur pollen, dries in the shade or dry after the robur florescence gathers micro-dew yellow-white flower fringe, puts into the refrigerator storage that temperature is less than-4 DEG C after then loading test tube.
3. pollen indoors artificial according to claim 1 raises the method for the very peaceful mite of Nissl, it is characterized in that: described raising platform is polyurethane foam and the black cloth that fully water suction put into by porcelain dish after sterilization or culture dish, the smooth scraps of paper are put repave last layer transparent plastic film after boiling water shower after, then evenly tremble the pollen of preservation on the scraps of paper, and surround the water segregation raising platform of surrounding formation with moistening cotton thread.
4. pollen indoors artificial according to claim 1 is raised Nissl and really to be pacified the method for mite, it is characterized in that: described in the cotton bunch that lays eggs bunch for some cotton silks or bar colligation are made.
5. pollen indoors artificial according to claim 1 raises the method for the very peaceful mite of Nissl, it is characterized in that: within 2 days ~ 3 days, change 1 pollen.
6. the pollen indoors artificial according to the arbitrary claim of claim 1-5 raises the method for the very peaceful mite of Nissl, it is characterized in that: described moist closet body of wall insulation material, glass two-layer warm-keeping glass, and avoid direct sunlight, first whitewash with lime water before raising, use 3% formalin spraying disinfection again, under 25 DEG C of conditions airtight more than 24 hours.
7. pollen indoors artificial according to claim 6 raises the method for the very peaceful mite of Nissl, it is characterized in that: keep receptacle temperature to be 20 ~ 25 DEG C, relative moisture 70% ~ 85%, light application time 8 hours, intensity of illumination 667 lux.
8. pollen indoors artificial according to claim 7 raises the method for the very peaceful mite of Nissl, it is characterized in that: described Nissl is really pacified, and to perch plant leaf blade be one or both in Japan, purple perilla to mite, gather the very peaceful mite of the more Nissl such as Japan, purple perilla in worm source from field and perch plant leaf blade, by No. 0 each worm state of writing brush picking, put down gently and raise on platform at water segregation.
9. pollen indoors artificial according to claim 1 raises the method for the very peaceful mite of Nissl, it is characterized in that: also comprise rejuvenation: gather the very peaceful mite of a certain amount of Nissl every year to field and become mite, raise together with, thus population is maintained vigour with the mite of indoor propagation.
CN201410647940.XA 2014-11-14 2014-11-14 Method for artificially feeding euseius nicholsi in pollen room Pending CN104430194A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107410224A (en) * 2017-09-09 2017-12-01 首伯农(北京)生物技术有限公司 A kind of Phyloseiulus nersimilis that obtains is cut open the belly the method for ovum
CN111351785A (en) * 2020-04-22 2020-06-30 磐安柔曼微生物科技有限公司 Different mite treatment methods effect detection device
CN113287576A (en) * 2021-05-13 2021-08-24 南昌大学 Method for artificial feeding of euseiulus nissei in simulated habitat

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CN103858828A (en) * 2014-03-27 2014-06-18 李庆 Method for breeding neoseiulus californicus through apricot blossom pollen

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Publication number Priority date Publication date Assignee Title
CN107410224A (en) * 2017-09-09 2017-12-01 首伯农(北京)生物技术有限公司 A kind of Phyloseiulus nersimilis that obtains is cut open the belly the method for ovum
CN111351785A (en) * 2020-04-22 2020-06-30 磐安柔曼微生物科技有限公司 Different mite treatment methods effect detection device
CN113287576A (en) * 2021-05-13 2021-08-24 南昌大学 Method for artificial feeding of euseiulus nissei in simulated habitat

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Application publication date: 20150325