CN103782963A - Amblyseius cucumeris artificial propagation method - Google Patents

Amblyseius cucumeris artificial propagation method Download PDF

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Publication number
CN103782963A
CN103782963A CN201210433421.4A CN201210433421A CN103782963A CN 103782963 A CN103782963 A CN 103782963A CN 201210433421 A CN201210433421 A CN 201210433421A CN 103782963 A CN103782963 A CN 103782963A
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CN
China
Prior art keywords
amblyseius cucumeris
artificial
cucumeris oudeman
amblyseius
oudeman
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Pending
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CN201210433421.4A
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Chinese (zh)
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李先琼
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Individual
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Individual
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Priority to CN201210433421.4A priority Critical patent/CN103782963A/en
Publication of CN103782963A publication Critical patent/CN103782963A/en
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Abstract

The invention provides an amblyseius cucumeris artificial propagation method which includes the steps of firstly, using Tenebrio molitor larvae or pupae, fine wheat bran and rape flower pollen as raw materials; secondly, cultivating amblyseius cucumeris. The method has the advantages that amblyseius cucumeris can propagate massively in a short time, and the method is simple in tool, easy to obtain artificial feed, low in cost, and capable of being widely popularized.

Description

The artificial fecundation method of amblyseius cucumeris oudeman
Technical field
The invention belongs to pest biological prevention field, particularly a kind of artificial fecundation method of amblyseius cucumeris oudeman.
Background technology
Amblyseius cucumeris oudeman ( amblyseius cucumeris(Oudemans) [Neoseiulus cucumeris]) belong to Phytoseiidae (Phytoseiid) Amblysius ( amblyseius) animal, can prey on two spotted spider mite on various farm-forestry crops, flowers, gardens, forest, fruit thing, tealeaves, blister mite, aleyrodid, thrips etc.Concrete prey has citrus red mite (crm), citrus rust mite, the citrus beginning tetranychid, Tetranychus urticae, Mite, Turkestan tetranychid, tetranychus viennensis, panonychus ulmi, Polyphagotarsonemus latus Banks, pink tea rust mite, coffee unguiculus mite, Schizotetranychus Nanjingensis, Phyllostachys pubscens Mazel, SchizotetranychusnanjingensisMaetYuan, bamboo thorn goitre mite, dust mite, alfalfa thrips, onion thrips, tyrophagus putrescentiae, the bright thrips of melon, primrose herbivore mite, yellow tea mite, priet mite, wood louse, two spotted spider mite, yellow twig, Bemisia tabaci, Frankliniella occidentalis, kamisawa tetranychus, than breathing out little melon mite, the full melon mite of apple, cotton spider mites, Pi Shi tetranychid, lupulus two spotted spider mite, and fungi etc.The plant being endangered, often has large-area blade withered, causes vegetables economic worth to reduce, and fruits output and quality decline, and reduce the commodity value of timber, has brought serious loss to plantation family.
The controlling object of amblyseius cucumeris oudeman has peach, Chinese chestnut, apple, corn, citrus, cotton, bergamot pear, lupulus, jujube, tealeaves, mao bamboon, rose, cucumber, capsicum, eggplant, tomato, pimento, leek, chrysanthemum, primrose, strawberry, pears, cherry, hawthorn, Lee, apricot, Malus spectabilis, Chinese pear-leaved crabapple, flowering plum, Chinese sorghum, beans, potato, shepherd's purse, water spinach, japanese bearbind, radish, Chinese cabbage, grape knotweed, Australia a thousand li wood, paper mulberry, mulberry tree, locust tree, elm etc.
At present, propagating artificially of amblyseius cucumeris oudeman obtained larger achievement, also a lot of about the research of its artificial feed, the breeding way of production, to have a liking for the mite classes such as squama mite, tyrophagus putrescentiae, Acarus siro as its feed, cultivating link is more loaded down with trivial details, is not easy to promote.
Summary of the invention
The present invention, on the basis of above technical deficiency, has proposed a kind of artificial fecundation method of the amblyseius cucumeris oudeman that is beneficial to popularization.
The present invention seeks to be achieved through the following technical solutions:
The artificial propagation techniques of amblyseius cucumeris oudeman of the present invention comprises the steps:
1), the configuration of artificial feed: utilize bloom first larva or pupa, thin wheat bran, rape petal pollen as raw material;
2), breeding amblyseius cucumeris oudeman.
The artificial feed of said method are that bloom first larva or pupa, thin wheat bran and rape petal pollen are as raw material, thin wheat bran with 15% bloom first larva or pupa+15% rape petal pollen+70% mixes, after configuring, be put under 6-8 ℃ of condition and store, before feeding, thaw to room temperature.
The artificial feed of said method are before configuration, and bloom first larva or pupa need be with 75% alcohol disinfecting 6min, the thin wheat bran 2h that need sterilize at 120 ℃, and rape petal pollen need grind with stone roller alms bowl.
The breeding of the amblyseius cucumeris oudeman of said method is, is inverted as hydrophobic platform with the interior culture dish of diameter 120mm or 150mm, and outer culture dish adds distilled water or cold boiling water, and the thickness of cultivation is 1-3cm.
1, the cultivating condition of said method is, temperature 27-33 ℃, and humidity 30-50%, ventilates, and light is even.Can raise (range of temperature 25-34 ℃, range of humidity variation 31-67%) summer in more nice and cool room.
Concrete modes of reproduction:
The artificial fecundation method of this enforcement amblyseius cucumeris oudeman comprises the following steps:
1, artificial feed
1), the raw-material preparation of artificial feed
Buy bloom first larva or pupa, in 75% alcohol, soak 6min, blot for subsequent use with blotting paper.The thin wheat bran 2h that sterilizes in 120 ℃ of baking ovens, cooling for subsequent use.Rape petal pollen is pulverized with stone roller alms bowl, pack in brown bottle for subsequent use.
2), the configuration of artificial feed
Take 15% bloom first larva or pupa, stir adult slurry with agitator, add 15% rape petal pollen, and 70% thin wheat bran mixes, pack in brown bottle, be put under 6-8 ℃ of condition and store, before feeding, thaw to room temperature.
2, the preparation of amblyseius cucumeris oudeman breeding utensil
75% alcohol disinfecting for the culture dish of diameter 120mm or 150mm, after drying, interior culture dish is inverted in outer culture dish.In culture dish, add outside distilled water or the cold boiling water of 1-2mm.
3, the breeding of amblyseius cucumeris oudeman
In interior culture dish, add appropriate amblyseius cucumeris oudeman, along with the feed of the 5-20g that increases input of quantity, keep thickness at 1-3cm.Cultivation ware is placed on to temperature 27-33 ℃, and humidity 30-50%, ventilates, and light is indoor uniformly, or in more nice and cool room, raises (range of temperature 25-34 ℃, range of humidity variation 31-67%) summer.Add feed every day, culture dish outside 2 days clean, and change the water in outer culture dish.In the time that thickness exceedes 3cm, point dish cultivation.Its culture efficiency is useful photo record all.
The above; only for preferably embodiment of the present invention, but protection scope of the present invention is not limited to this, is anyly familiar with those skilled in the art in the scope that the present invention relates to; the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.

Claims (7)

1. the artificial fecundation method of amblyseius cucumeris oudeman, its feature comprises following step:
1), the configuration of artificial feed: utilize bloom first larva or pupa, thin wheat bran, rape petal pollen as raw material;
2), breeding amblyseius cucumeris oudeman.
2. the artificial fecundation method of amblyseius cucumeris oudeman as claimed in claim 1, is characterized in that artificial feed are that the thin wheat bran of 15% bloom first larva or pupa slurry+15% rape petal pollen+70% mixes.
3. the artificial fecundation method of amblyseius cucumeris oudeman as claimed in claim 1, the cultivation diameter that it is characterized in that amblyseius cucumeris oudeman is the culture dish cultivation of 120mm or 150mm, with after 75% sterilization, interior culture dish is inverted as hydrophobic platform, in outer culture dish, add distilled water or cold boiling water.
4. the artificial fecundation method of amblyseius cucumeris oudeman as claimed in claim 1 or 2, is characterized in that in artificial feed that bloom first larva or pupa need be with after 75% alcohol disinfecting 6min, stirs into pulpous state with agitator; The thin wheat bran 2h that sterilizes in the baking oven of 120 ℃; Rape petal pollen grinds with stone roller alms bowl.
5. the artificial fecundation method of amblyseius cucumeris oudeman as claimed in claim 1 or 2, is characterized in that being placed under 6-8 ℃ of condition and storing after artificial feed configure; Before feeding, thaw to room temperature.
6. the artificial fecundation method of the amblyseius cucumeris oudeman as described in claim 1 or 3, the cultivating condition that it is characterized in that amblyseius cucumeris oudeman is temperature 27-33 ℃, and humidity 30-50%, ventilates, and light is even; Cultivation thickness is 1-3cm.
7. can in more nice and cool room, raise (range of temperature 25-34 ℃, range of humidity variation 31-67%) summer.
CN201210433421.4A 2012-11-05 2012-11-05 Amblyseius cucumeris artificial propagation method Pending CN103782963A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201210433421.4A CN103782963A (en) 2012-11-05 2012-11-05 Amblyseius cucumeris artificial propagation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201210433421.4A CN103782963A (en) 2012-11-05 2012-11-05 Amblyseius cucumeris artificial propagation method

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CN103782963A true CN103782963A (en) 2014-05-14

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104351146A (en) * 2014-11-26 2015-02-18 曾少兰 Method for culturing neoseiulus cucumeris oudermans through pollen mixture
CN104430194A (en) * 2014-11-14 2015-03-25 中国农业科学院柑桔研究所 Method for artificially feeding euseius nicholsi in pollen room
CN105746254A (en) * 2016-03-14 2016-07-13 福建艳璇生物防治技术有限公司 Method for preventing red spiders, thrips and aleyrodids on strawberries by amblyseius cucumeris

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104430194A (en) * 2014-11-14 2015-03-25 中国农业科学院柑桔研究所 Method for artificially feeding euseius nicholsi in pollen room
CN104351146A (en) * 2014-11-26 2015-02-18 曾少兰 Method for culturing neoseiulus cucumeris oudermans through pollen mixture
CN105746254A (en) * 2016-03-14 2016-07-13 福建艳璇生物防治技术有限公司 Method for preventing red spiders, thrips and aleyrodids on strawberries by amblyseius cucumeris

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Application publication date: 20140514