CN109156115A - A kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout - Google Patents
A kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout Download PDFInfo
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- CN109156115A CN109156115A CN201811131902.3A CN201811131902A CN109156115A CN 109156115 A CN109156115 A CN 109156115A CN 201811131902 A CN201811131902 A CN 201811131902A CN 109156115 A CN109156115 A CN 109156115A
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- seed
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- gymnadenia conopsea
- bacterium colony
- brown
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of fungies that promotion orchidaceae medicinal plant gymnadenia conopsea seed is sprouted, the bacterium colony of the fungi is grown rapidly in PDA culture medium, there is a large amount of cotton-shaped aerial hyphae in the bacterium colony, white brown is presented in the bacterium colony front, the back side presents light brown, dark brown is presented in the aerial hyphae of aging, the colony edge is neat, became uneven is even, the speed of growth of the mycelia is 0.54~0.57mm/h, the diameter of the mycelia is 2.71~3.72 μm, under microscope can clear view to membrane configuration, the nearly right angle of branch, have no that zoogamy spore or asexual chlamydospore generate.Fungi of the present invention can effectively facilitate the sprouting of gymnadenia conopsea seed, can be applied in gymnadenia conopsea seedling breeding from now on.
Description
Technical field
The invention belongs to field of biotechnology more particularly to it is a kind of promote orchidaceae medicinal plant gymnadenia conopsea seed sprout it is true
Bacterium.
Background technique
Orchid family is one of maximum, most multifarious section in flowering plant, and there are about 26,000 kinds for whole world orchid, extensively
It is general to be distributed in various terrestrial ecosystems.The numerous species in orchid have become rare and endangered species at present, are states
The monoid to receive much attention in bio-diversity conservation on border.The Chinese government also attaches great importance to the protection of orchid, orchid
It is put into " national key protected wild plants register " entirely, and " People's Republic of China's conservation of wild plant item will be included in
Example " protection category.
Gymnadenia conopsea is raw type orchid a kind ofly, China be sporadicly distributed in Heilungkiang, Jilin, Liaoning, Hebei, Shanxi,
The grassy marshland of the height above sea level 265-4700m such as Shaanxi, Gansu, Sichuan, Yunnan and the Inner Mongol and Tibet Autonomous Region, hillside, grass plot, river
Between paddy and shrubbery.Gymnadenia conopsea is also the common medicine of anaesthetic and Tibetan medicine as Chinese tradition Chinese medicine material, has very high medicinal valence
Value, modern medicine study shows that gymnadenia conopsea contains various bioactive components, has tonifying kidney and benefiting sperm, anti-oxidant, antiviral, anti-ageing
Always, antiallergy, preventing gastric ulcer, raising immunity, calming soporific, enhancing memory and other effects.Although gymnadenia conopsea setting percentage is high, numerous
It is low to grow rate, and in recent years by exhaustive exploitation, quantity is sharply reduced, the destruction in habitat in addition causes wild resource exhausted, by
" relating to danger wild animals and plants kind international trade pact " (CITES) is classified as II grades of rare medicinal plants, by " Chinese species red name
Record " it is set to nearly danger kind, it can see that the protection of gymnadenia conopsea resource and breeding seem extremely urgent completely.
Research to orchid propagation method mainly has sexual propagation and two kinds of vegetative propagation.And under field conditions (factors),
For gymnadenia conopsea in vegetative propagation, veteran joins root only 1 small gymnadenia conopsea of hyperplasia, and when small gymnadenia conopsea is grown up, veteran's ginseng is withered, average to breed
Rate multiple is lower than 2；Sexual propagation in relation to gymnadenia conopsea, is also rarely reported at present.In order to solve the problems, such as the shortage of resources of gymnadenia conopsea, China
Some scholars attempt the resource regeneration that gymnadenia conopsea is realized by tissue cultures or cell culture mode, however these research distances are true
Positive industrialized production is also that fashion is remote.The seed of orchid is small, itself can not store nutrient, and the sprouting stage fully relies on
Mycorrhizal fungi provides nutrition for it.The characteristics of depending on fungi based on the germination of arethusa seeds stage, research promote orchid family to plant
The work for the excellent fungal bacterial strain (being commonly called as Germination Strain) that species is sprouted just is particularly important.Realize that gymnadenia conopsea is total using Germination Strain
The raw sexual propagation mode sprouted will become the effective means for realizing the child care of gymnadenia conopsea seed and its resources.
Summary of the invention
The purpose of the present invention is obtain a kind of fungi that promotion orchidaceae medicinal plant gymnadenia conopsea seed is sprouted.
The purpose of the present invention is what is be achieved through the following technical solutions, a kind of promotion orchidaceae medicinal plant gymnadenia conopsea seed sprouting
Fungi, the bacterium colony of the fungi grows rapidly in PDA culture medium, and the bacterium colony has a large amount of cotton-shaped aerial hyphae, institute
It states bacterium colony front and white brown is presented, light brown, the aerial hyphae presentation dark brown of aging is presented in the back side, and the colony edge is whole
Together, became uneven is even, and the speed of growth of the mycelia is 0.54~0.57mm/h, and the diameter of the mycelia is 2.71~3.72 μ
M, under microscope can clear view to membrane configuration, the nearly right angle of branch has no that zoogamy spore or asexual chlamydospore generate.
Preferably, the edge of the bacterium colony is relatively thin.
Compared with prior art, modern the invention has the following advantages: the fungi can effectively facilitate the sprouting of gymnadenia conopsea seed
After can be applied in gymnadenia conopsea seedling breeding.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
Embodiment 1
A kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout, the bacterium colony of the fungi are raw in PDA culture medium
Rapidly, there is a large amount of cotton-shaped aerial hyphae in the bacterium colony to length, and white brown is presented in the bacterium colony front, and back side presentation is light brown,
Dark brown is presented in the aerial hyphae of aging, and the colony edge is neat, and became uneven is even, and the speed of growth of the mycelia is 0.54
~0.57mm/h, the diameter of the mycelia are 2.71~3.72 μm, under microscope can clear view to membrane configuration, branch is closely straight
Angle has no that zoogamy spore or asexual chlamydospore generate.
Preferably, the edge of the bacterium colony is relatively thin.
Bacterial strain and gymnadenia conopsea seed are co-cultured, the sprouting of gymnadenia conopsea seed can be effectively facilitated, and the control group gymnadenia conopsea of fungi is not added
Seed does not have sprouting sign.
Used medium: OMA (oat medium)
Condition of culture: 15-22 DEG C
Under microscope, the feature that there are gymnadenia conopsea seed Orchid Seeds to share is observed, seed is small, it can be seen that outside
It plants skin and plants the embryo under suitcase is wrapped up in.Gymnadenia conopsea seed shape is in shuttle-type, in laboratory conditions, 23 days left sides of seed symbiotic germination
It is right, so that it may see that embryo expands, it is prominent to break in the seed coat, protocorm is formed, is sprouted to 2 grades, surface forms epidermal hair or rhizoid phenomenon.
Subsequent protocorm volume constantly increases and starts to differentiate protomeristem, sprouts to 3 grades；Sow about 40 days left sides
The right side differentiates scale or scultellum.
At 2 months, start to differentiate leaf, reaches 4 grades；At 3 months, leaf constantly extends, and differentiates root, forms seedling.
Later, seedling can also constantly continued growth, stretch out two panels leaf.
Fungi of the present invention can effectively facilitate the sprouting of gymnadenia conopsea seed, can be applied in gymnadenia conopsea seedling breeding from now on.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (2)
1. a kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout, characterized in that the bacterium colony of the fungi is cultivated in PDA
It is grown on base rapidly, the bacterium colony has a large amount of cotton-shaped aerial hyphae, and white brown is presented in the bacterium colony front, and the back side presents shallow
Dark brown is presented in the aerial hyphae of brown, aging, and the colony edge is neat, and became uneven is even, the speed of growth of the mycelia
For 0.54~0.57mm/h, the diameter of the mycelia is 2.71~3.72 μm, under microscope can clear view arrive membrane configuration, divide
The nearly right angle of branch has no that zoogamy spore or asexual chlamydospore generate.
2. the fungi according to claim 1 for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout, characterized in that the bacterium colony
Edge it is relatively thin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811131902.3A CN109156115A (en) | 2018-09-27 | 2018-09-27 | A kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811131902.3A CN109156115A (en) | 2018-09-27 | 2018-09-27 | A kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout |
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| CN109156115A true CN109156115A (en) | 2019-01-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811131902.3A Pending CN109156115A (en) | 2018-09-27 | 2018-09-27 | A kind of fungi for promoting orchidaceae medicinal plant gymnadenia conopsea seed to sprout |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111387057A (en) * | 2020-04-24 | 2020-07-10 | 四川省农业科学院生物技术核技术研究所 | Gymnadenia conopsea sterile seeding culture medium |
| CN114456951A (en) * | 2022-01-28 | 2022-05-10 | 中国医学科学院药用植物研究所 | Cucumaria fungal strain for promoting growth of gymnadenia conopsea, and method and application thereof |
-
2018
- 2018-09-27 CN CN201811131902.3A patent/CN109156115A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111387057A (en) * | 2020-04-24 | 2020-07-10 | 四川省农业科学院生物技术核技术研究所 | Gymnadenia conopsea sterile seeding culture medium |
| CN111387057B (en) * | 2020-04-24 | 2022-10-18 | 四川省农业科学院生物技术核技术研究所 | Gymnadenia conopsea sterile seeding culture medium |
| CN114456951A (en) * | 2022-01-28 | 2022-05-10 | 中国医学科学院药用植物研究所 | Cucumaria fungal strain for promoting growth of gymnadenia conopsea, and method and application thereof |
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Application publication date: 20190108 |
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