CN104969750A - Fungus inoculation method for improving survival rate of cuttage of Chinese yews and quality of seedlings - Google Patents
Fungus inoculation method for improving survival rate of cuttage of Chinese yews and quality of seedlings Download PDFInfo
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- CN104969750A CN104969750A CN201510359280.XA CN201510359280A CN104969750A CN 104969750 A CN104969750 A CN 104969750A CN 201510359280 A CN201510359280 A CN 201510359280A CN 104969750 A CN104969750 A CN 104969750A
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- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a rhizosphere fungus inoculation method for improving the survival rate of the cuttage of Chinese yews and the quality of seedlings. The fungus inoculation method for improving the survival rate of the cuttage of the Chinese yews and the quality of the seedlings comprises the steps of the cultivation and the growth of trichoderma Trichoderma sp. bacterial strains and IRB54 bacterial strains in corn straws, the preparation of a mycelium seedbed containing the IRB54 bacterial strains, the clipping and the preparation of appropriate cuttings, the rooting power treatment on the cuttings, the cuttage of the cuttings onto the prepared seedbed containing mycelia, sufficient flood irrigation or spraying, and final normal cutting seedling management until the front cuttings grow into suitable heights according with the strong seedling transplantation standard. Compared with a traditional cuttage method, by means of the fungus inoculation method for improving the survival rate of the cuttage of the Chinese yews and the quality of the seedlings, the survival rate of the cuttage is improved, the quality of the seedlings is improved, the survival rate of transplanting and the growth vigor and the growth rate of the transplanted seedlings are improved, and the disease resistance of the seedlings is improved.
Description
Technical field
The invention belongs to technical field of plant cultivation, be specifically related to a kind of fungi inoculation method improving Yew cuttage survival rate and seedling quality.
Background technology
Taxus is in first-grade state protection plant, and wild quantity is extremely rare, and it not only has important scientific research value; also be the raw material that the important chemotherapeutic drug Paclitaxel of cancer extracts simultaneously; but due to it, the amount of producing seeds is few naturally, and poor growth, make its field child care and artificial propagation speed slow.Therefore, cuttage becomes the Main Means of Chinese yew artificial propagation, but, the breeding of current Yew cuttage exists that cuttage branch survival rate is low, seedling growing way is weak, transplanting survival rate is low, growing way is weak and the problem such as easy infection root disease, therefore, how to overcome by technological means the deficiency solving above-mentioned cuttage seeding and become Chinese yew seeling industry key issue urgently to be resolved hurrily.
Wood is mould in recent years always by the important fungi as biocontrol of plant disease, it is harmless to root system of plant, growth is fast, and appropriate plant auxins material can be produced, promote the growth of root system of plant, but in actual production, also do not see about Trichoderma being inoculated into the report carrying out cuttage in matrix.
Summary of the invention
The object of this invention is to provide a kind of fungi inoculation method improving Yew cuttage survival rate and seedling quality, solve that the Yew cuttage branch survival rate existed in prior art is low, growing way is weak, transplanting survival rate is low and the problem of easy infection root disease.
The technical solution adopted in the present invention is, a kind of fungi inoculation method improving Yew cuttage survival rate and seedling quality, comprises the following steps:
1), pretreatment is carried out to maize straw;
2), in maize straw, fungi strain cultivation is carried out;
3) fungi strain after cultivating is inoculated in cuttage seedbed, the suitable branch of clip is also cut into suitable cutting, carries out cuttage after root-inducing powder process in the seedbed of inoculated fungi, then water once permeable to cutting;
4) carry out general cuttage and seedling culture management, transplant until cuttage seeding grows to meet or go out garden requirement.
Feature of the present invention is also,
Fungi is wooden mould Trichoderma sp. bacterial strain IRB54.
The pretreatment of maize straw for pulverize maize straw, then autoclaving 1 hour under 120 DEG C of conditions.
Seedbed matrix composition be the peat composed of rotten mosses and river sand, and with 0.5% potassium permanganate thorough disinfection; Fully mix with bacterial classification after the abundant airing of matrix after sterilization, then make wide 120 centimetres, long 10 meters of seedbeds.
Branch for cuttage is the raw lignification branch of 1-2.
The length of cutting is 10-15 centimetre, and lower cut is cut into the shape of a hoof.
The concentration of root-inducing powder is 200ppm.
The proportion of composing of matrix is the peat composed of rotten mosses: river sand=1ml:2ml.
Abundant airing refers to the 5-7 days that to be fully exposed to the sun under the sun.
The thickness of medium of seedling bed is 15-20 centimetre, does substrate with apertured plastic cloth.
The invention has the beneficial effects as follows: the present invention is by the wooden mould method of inoculation in Chinese yew matrix, and improve the rooting rate of Yew cuttage branch, survival rate and seedling quality, method is simple, successful.The present invention inoculates wood and mouldly also improves Yew cuttage transplantation of seedlings survival rate and disease resistance in Chinese yew matrix, and after its cuttage seeding is transplanted, growing way is due to general cuttage seeding.
Accompanying drawing explanation
Fig. 1 is that fungus colony of the present invention is at 23-24 DEG C of upgrowth situation figure.
Wherein, A) be the colonial morphology of growth after 24 hours; B) be the colony growth colonial morphology of 72 hours; C) be the growth colonial morphology of 96 hours.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
The invention provides a kind of fungi inoculation method improving Yew cuttage survival rate and seedling quality, comprise the following steps:
1), to maize straw carry out pretreatment, be specially: maize straw is pulverized, then autoclaving 1 hour under 120 DEG C of conditions;
2), in maize straw, fungi strain cultivation is carried out, wherein, fungi is that wooden mould Trichoderma sp. bacterial strain IRB54 is (open, bibliography: Li et al. (2015) Isolation and Identification ofa 10-Deacetyl Baccatin-III-Producing Endophyte from Taxus wallichiana.Appl.Biochem.Biotechnol.175:2224-2231), fungus colony is at 23-24 DEG C of upgrowth situation figure as shown in Figure 1;
3) fungi strain after cultivation is inoculated in cuttage seedbed, the suitable branch of clip is also cut into suitable cutting, cuttage after root-inducing powder process is carried out in the seedbed of inoculated fungi to cutting, then water once permeable, wherein, seedbed matrix composition be the peat composed of rotten mosses and river sand, and with 0.5% potassium permanganate thorough disinfection; Fully mix with bacterial classification after the abundant airing of matrix after sterilization, then make wide 120 centimetres, long 10 meters of seedbeds; Branch for cuttage is the raw lignification branch of 1-2; The length of cutting is 10-15 centimetre, and lower cut is cut into the shape of a hoof; The concentration of root-inducing powder is 200ppm; The proportion of composing of matrix is the peat composed of rotten mosses: river sand (volume ratio)=1:2; Abundant airing refers to the 5-7 days that to be fully exposed to the sun under the sun, remains to eliminate potassium permanganate as far as possible; The thickness of medium of seedling bed is 15-20 centimetre, does substrate with apertured plastic cloth.
4) carry out general cuttage and seedling culture management, transplant until cuttage seeding grows to meet or go out garden requirement.
Why Trichoderma can improve Yew cuttage shoot survival percent, rooting rate, seedling quality, improve cuttage seeding survival rate, growth potential and disease resistance mainly because following reason: first trichoderma mycelia can discharge a certain amount of growth hormone in process of growth, it can induce the adventive root of cuttage branch to produce, increase the number of adventive root root, and accelerate the growth of new root, and therefore facilitate cuttage seeding aerial growth and transplanting survival rate.Woodenly in addition mould disease is not formed to root system of plant; and have and nourish and grow fast in the mould tool of wood; a Trichoderma protective layer can be formed at plant root rapidly, so just prevent other harmful fungoids to the parasitism of seedling root, thus enhance the disease resistance of cuttage seeding.
Table 1. inoculate the cuttage seedbed of Trichoderma produce cuttage seeding and contrast seedbed produce the comparison of cuttage seeding
This table the data obtained is all from the mean value of repeated test (comprise process and contrast each 5 repeated tests), as can be seen from Table 1, the cuttage seedbed of inoculation Trichoderma produce in cuttage seeding and prior art contrast seedbed produce the comparison of cuttage seeding, its survival rate, rooting rate, transplanting survival rate etc. are by being significantly improved.
The present invention by inoculating the matrix environment that Trichoderma improves the branch of cuttage in Chinese yew cutting cutting medium, not only increase rooting rate and the survival rate of cuttage branch, also improve seedling quality, the transplanting simultaneously also improving Yew cuttage seedling becomes rate, transplants rear growing way and disease resistance.
Claims (10)
1. improve a fungi inoculation method for Yew cuttage survival rate and seedling quality, it is characterized in that, comprise the following steps:
1), pretreatment is carried out to maize straw;
2), in maize straw, fungi strain cultivation is carried out;
3) fungi strain after cultivating is inoculated in cuttage seedbed, the suitable branch of clip is also cut into suitable cutting, carries out cuttage after root-inducing powder process in the seedbed of inoculated fungi, then water once permeable to cutting;
4) carry out general cuttage and seedling culture management, transplant until cuttage seeding grows to meet or go out garden requirement.
2. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, described fungi is wooden mould Trichoderma sp. bacterial strain IRB54.
3. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the pretreatment of described maize straw for pulverize maize straw, then autoclaving 1 hour under 120 DEG C of conditions.
4. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the matrix in described seedbed composition is the peat composed of rotten mosses and river sand, and with 0.5% potassium permanganate thorough disinfection; Fully mix with bacterial classification after the abundant airing of matrix after sterilization, then make wide 120 centimetres, long 10 meters of seedbeds.
5. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the described branch for cuttage is the raw lignification branch of 1-2.
6. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the length of described cutting is 10-15 centimetre, and lower cut is cut into the shape of a hoof.
7. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the concentration of described root-inducing powder is 200ppm.
8. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the proportion of composing of described matrix is the peat composed of rotten mosses: river sand=1ml:2ml.
9. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, described abundant airing refers to the 5-7 days that to be fully exposed to the sun under the sun.
10. the fungi inoculation method of raising Yew cuttage survival rate according to claim 1 and seedling quality, is characterized in that, the thickness of described medium of seedling bed is 15-20 centimetre, does substrate with apertured plastic cloth.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105767012A (en) * | 2016-04-18 | 2016-07-20 | 杭州富阳飞博科技有限公司 | Accelerant facilitating plant cutting rooting |
| CN106518350A (en) * | 2016-10-27 | 2017-03-22 | 六安市振清生态农业有限公司 | Nutritive organic matrix for taxus chinensis planting |
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| JPS62262918A (en) * | 1986-05-10 | 1987-11-16 | ロンタイ株式会社 | Vegetation plate and its production |
| CN1965638A (en) * | 2005-11-15 | 2007-05-23 | 方立成 | Compact type substrate seedling nursery culture pan |
| CN101254999A (en) * | 2008-03-28 | 2008-09-03 | 中国科学院过程工程研究所 | Preparation of belt seeding carrier by steam explosive-crushing straw solid-state fermentation |
| CN102960149A (en) * | 2012-11-27 | 2013-03-13 | 普定县绿源苗业开发有限公司 | Taxus chinensis cuttage cultivation method |
| CN104170558A (en) * | 2014-08-12 | 2014-12-03 | 安徽永兴岛生态农业科技有限公司 | Salvia miltiorrhiza planting method |
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2015
- 2015-06-25 CN CN201510359280.XA patent/CN104969750A/en active Pending
Patent Citations (5)
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| JPS62262918A (en) * | 1986-05-10 | 1987-11-16 | ロンタイ株式会社 | Vegetation plate and its production |
| CN1965638A (en) * | 2005-11-15 | 2007-05-23 | 方立成 | Compact type substrate seedling nursery culture pan |
| CN101254999A (en) * | 2008-03-28 | 2008-09-03 | 中国科学院过程工程研究所 | Preparation of belt seeding carrier by steam explosive-crushing straw solid-state fermentation |
| CN102960149A (en) * | 2012-11-27 | 2013-03-13 | 普定县绿源苗业开发有限公司 | Taxus chinensis cuttage cultivation method |
| CN104170558A (en) * | 2014-08-12 | 2014-12-03 | 安徽永兴岛生态农业科技有限公司 | Salvia miltiorrhiza planting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105767012A (en) * | 2016-04-18 | 2016-07-20 | 杭州富阳飞博科技有限公司 | Accelerant facilitating plant cutting rooting |
| CN105767012B (en) * | 2016-04-18 | 2018-03-27 | 河南莱泰园林股份有限公司 | A kind of accelerator beneficial to plant cuttage root-taking |
| CN106518350A (en) * | 2016-10-27 | 2017-03-22 | 六安市振清生态农业有限公司 | Nutritive organic matrix for taxus chinensis planting |
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