CN103508788A - Domestication cultivation medium for bletilla striata tissue culture seedlings - Google Patents
Domestication cultivation medium for bletilla striata tissue culture seedlings Download PDFInfo
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- CN103508788A CN103508788A CN201310392377.1A CN201310392377A CN103508788A CN 103508788 A CN103508788 A CN 103508788A CN 201310392377 A CN201310392377 A CN 201310392377A CN 103508788 A CN103508788 A CN 103508788A
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Abstract
The invention provides a domestication cultivation medium for bletilla striata tissue culture seedlings. The domestication cultivation medium is compounded by mixing disease-free loam, humus soil and perlite according to certain volume percentages and stirring. The medium of the invention is reasonable in formula material selection and ratio, comprises the simple and efficient components, gives full play to water retaining, ventilation and nutrition supplement which are needed by domestication cultivation, is wide in employed material resource and substantially reduced in cost, and possesses extremely strong popularization value. The bletilla striata tissue culture seedlings bred by employing the domestication cultivation medium of the invention are good in growth vigor, are strengthened and aligned, and the finally obtained bletilla striata plants are flourished in rhizome and strong. The domestication time by employing the domestication cultivation medium is relatively short, and only after 2-4 months, the seedlings can be transplanted into a field for cultivation with a survival rate of 85% or more, and the production efficiency is substantially improved.
Description
Technical field
The present invention relates to a kind of bletilla striata tissue cultured seedling domesticating and cultivating matrix.
Background technology
The bletilla striata is the orchid family bletilla striata platymiscium, with stem tuber, is used as medicine, and has the effects such as tonifying lung hemostasis, detumescence and promoting granulation.In recent years, along with going deep into of bletilla striata efficacy study, the market requirement is increasing, and the destroyed property of wild resource ground is excavated, and can not meet market demand far away.Bletilla striata division propagation efficiency is low, of poor benefits.Through research, the artificial medium aseptic seeding technology of the bletilla striata is quite ripe, and germination rate is higher.But in domestication link, because matrix selection is improper or component proportions is not right, after bletilla striata tissue cultured seedling bottle outlet, growth conditions is bad, surviving rate is lower, and this has greatly limited and has utilized tissue culture technique large-scale production bletilla striata seedling.Therefore, extremely urgent for the research of bletilla striata tissue cultured seedling domestication growth period condition.
A kind of bletilla group as disclosed in CN103222421A is trained bulb acclimation method, discloses the domestication matrix of the bletilla striata, and the component of domestication matrix is wood sawdust, vermiculite and the broken bark becoming thoroughly decomposed, and the mass ratio of wood sawdust, vermiculite and broken bark is 3:1:1.Adopt the disclosed domestication matrix of this scheme, must transplant and can carry out field production in 3～6 months, the domestication time is longer.
Summary of the invention
In order to solve the problems of the technologies described above, the present invention solves the problem that lacks efficient bletilla striata tissue cultured seedling domesticating and cultivating matrix in prior art.
A tissue cultured seedling domesticating and cultivating matrix is to be formed according to following volume percent is composite by anosis loam, vegetable mould, perlite:
Anosis loam 20%～30%, vegetable mould 20%～30%, perlite 40%～60%.
Further, the volume percent of described raw material is:
Anosis loam 22.5%～27.5%, vegetable mould 22.5%～27.5%, perlite 45%～55%.
Further, the volume percent of described raw material is:
Anosis loam 25%, vegetable mould 25%, perlite 50%.
The preparation method of above-mentioned bletilla striata tissue cultured seedling domesticating and cultivating matrix is: by each raw material according to volume percent mixing and stirring.
Before using this matrix, can adjust EC value lower than 1.0ms/cm, then apply by measuring EC value, be conducive to improve the survival rate of bletilla striata tissue cultured seedling.EC value is for measuring the soluble ion concentration of planting medium (domesticating and cultivating matrix of the present invention), because the solubility salt of high density can make bletilla striata tissue cultured seedling sustain damage or cause the death of bletilla striata tissue cultured seedling root system.EC value, higher than 1.0ms/cm, may form reverse osmotic pressure, and the moisture in root system is cemented out, and makes tip of a root browning or withered, unfavorable to the g and D of the bletilla striata tissue cultured seedling in seedling stage.Regulate the conductivity that the method for EC value is test matrix, to adjust its soluble salt concentration, make its EC value lower than 1.0ms/cm.
The present invention's anosis loam used is commercially available prod.Because bletilla striata tissue cultured seedling resistibility is poor, easily infected, need a large amount of nutrition to grow simultaneously, thus must can be transplanted into land for growing field crops through domesticating and cultivating, otherwise survival rate is not high; Therefore, the domestication matrix of bletilla striata tissue cultured seedling need to possess the feature without infectious and trophicity.The anosis loam that the present invention adopts is both for bletilla striata tissue cultured seedling provides good soil fertility condition, the situation of having avoided again the germ in common loam to cause bletilla striata tissue cultured seedling to be died of illness.Anosis loam of the present invention is taken from the higher soil of nutrient that the equal unrooted stem disease evil of front two batches of crops of planting on land for growing field crops occurs.
The present invention's perlite used is commercially available prod.Perlite is a kind of cleaning, neutral dielectric material almost, has light weight, characteristic that ventilation property is strong.Perlitic stable physical-chemical indexes, a large amount of moisture and nutritive ingredient can be preserved in ，Qi space simultaneously, supply with for a long time bletilla striata tissue cultured seedling growth needs, and can effectively control fertilizer efficiency and fertile degree, and (being suitable for and anosis loam collocation), can be used for permanent cultivated plant.Perlitic porousness has greatly promoted root growth and the growth of bletilla striata tissue cultured seedling, and bletilla striata tissue cultured seedling is had to fabulous fixed action.
The present invention's vegetable mould used is commercially available prod.Vegetable mould is to screen out thick or meticulous waste material after being mixed by the plant material rotting and all kinds of organic waste, and the mixture using after pyrogenic distillation and disinfection, for example vegetable mould of the residual leaf of the deadwood of topsoil trees through forming after putrid fermentation over a long time in forest.Complicated component humous, slant acidity, has the features such as trophic function and sharp water moisture-keeping, fertilizer-keeping.Because water-retentivity humous is strong, so its ventilation property is not ideal enough.This is that its venting capability is poor because of the material of common good water-retaining property.Vegetable mould and perlite are share, can guarantee that domesticating and cultivating matrix has water-retentivity and ventilation property concurrently.The perlite that proportioning is suitable and vegetable mould can make matrix obtain appropriate water-retentivity and ventilation property, and suitable perlite, vegetable mould and the anosis loam of proportioning can make matrix obtain good water-retentivity, ventilation property and fertility.The reasonable raw material proportioning that the present invention adopts is effective, can guarantee that the water-retentivity of domesticating and cultivating matrix and ventilation property are suitable, can meet bletilla striata demand to moisture and oxygen in cycle process.
Angle from habit, the bletilla striata is mainly grown under hillside thick grass, cheuch and the sparse woods of high Mountain area of height above sea level 500～1500m, and how the environment of liking warm, cool place to fail in love, grow on fertile, loose and well-drained sand loam or detritus loam.Visible, improve the survival rate of bletilla striata tissue cultured seedling, can imitate the matrix that soil in the self-sow environment of the bletilla striata is prepared domesticating and cultivating.The present invention has adopted sandy soil (perlite is alternative) and the detritus soil (vegetable mould) that is suitable for bletilla striata growth just, and the good anosis loam of fertility, by suitable proportioning, obtains a kind of high-quality, efficient bletilla striata tissue cultured seedling domesticating and cultivating matrix.
Compared with prior art, the beneficial effect that the present invention reaches is: substrate formula selection and reasonable ratio, form simple and efficient, can give full play to the required water conservation of domesticating and cultivating, ventilative and trophism, and material therefor wide material sources, cost significantly reduces, and has very strong promotional value.The bletilla striata tissue cultured seedling growth potential that adopts domesticating and cultivating matrix of the present invention to breed is good, and seedling is strong, seedling is neat, and the final bletilla striata plant roots and stems obtaining is flourishing, robust plant.Adopt domesticating and cultivating matrix of the present invention to tame required time shorter, only need within 2～4 months, can move into field production, and surviving rate reaches more than 85%, greatly improved production efficiency.
Embodiment
In order to deepen that the present invention is understood, below in conjunction with embodiment, the present invention is described in further detail, and this embodiment only, for explaining the present invention, does not form limiting the scope of the present invention.The modification of making on basis of the present invention or improvement, if it will be apparent to those skilled in the art that, belong to the scope of protection of present invention.
Embodiment 1
Substrate formula: anosis loam 20%, vegetable mould 20%, perlite 60%.
Preparation method: by each raw material according to volume percent mixing and stirring after, measure EC value, adjustment EC value is lower than 1.0ms/cm.
Application: bletilla striata tissue cultured seedling is implanted in domesticating and cultivating matrix, cultivated about 4 months, can be transplanted into large Tanaka.
Embodiment 2
Substrate formula: anosis loam 30%, vegetable mould 30%, perlite 40%.
Preparation method: by each raw material according to volume percent mixing and stirring.
Application: bletilla striata tissue cultured seedling is implanted in domesticating and cultivating matrix; Or measure the EC value of matrix and adjust EC value lower than 1.0ms/cm after, by bletilla striata tissue cultured seedling implantation domesticating and cultivating matrix; Cultivate about 4 months, can be transplanted into large Tanaka.
Embodiment 3
Substrate formula: anosis loam 25%, vegetable mould 25%, perlite 50%.
Preparation method: by each raw material according to volume percent mixing and stirring.
Application: bletilla striata tissue cultured seedling is implanted in domesticating and cultivating matrix; Or measure the EC value of matrix and adjust EC value lower than 1.0ms/cm after, by bletilla striata tissue cultured seedling implantation domesticating and cultivating matrix; Cultivate about 2 months, can be transplanted into large Tanaka.
Embodiment 4
Substrate formula: anosis loam 22.5%, vegetable mould 22.5%, perlite 55%.
Preparation method: by each raw material according to volume percent mixing and stirring after, measure EC value, adjustment EC value is lower than 1.0ms/cm.
Application: bletilla striata tissue cultured seedling is implanted in domesticating and cultivating matrix; Or measure the EC value of matrix and adjust EC value lower than 1.0ms/cm after, by bletilla striata tissue cultured seedling implantation domesticating and cultivating matrix; Cultivate about 3 months, can be transplanted into large Tanaka.
Embodiment 5
Substrate formula: anosis loam 27.5%, vegetable mould 27.5%, perlite 45%.
Preparation method: by each raw material according to volume percent mixing and stirring.
Application: bletilla striata tissue cultured seedling is implanted in domesticating and cultivating matrix; Or measure the EC value of matrix and adjust EC value lower than 1.0ms/cm after, by bletilla striata tissue cultured seedling implantation domesticating and cultivating matrix; Cultivate about 3 months, can be transplanted into large Tanaka.
Adopt the bletilla striata tissue cultured seedling of matrix domesticating and cultivating of the present invention after transplanting, surviving rate is up to more than 97%, and average yield per mu reaches 200～300 kilograms, by 100 yuan of calculating of fresh goods per kilogram, per mu yield value reaches 20000～30000 yuan, and economic benefit is good, has promotional value.
Test example
300 strains of bletilla striata tissue cultured seedling are divided into 6 groups at random, every group of 50 strains; Wherein, bletilla striata tissue cultured seedling in group 1 is implanted in the disclosed bletilla striata domestication matrix of CN103222421A (contrast media), bletilla striata tissue cultured seedling in 6 of group 2～organize is implanted respectively in the domesticating and cultivating matrix described in the embodiment of the present invention 1～5, after 3 months, each group bletilla striata tissue cultured seedling is transplanted to respectively to large Tanaka from matrix, statistics survival rate, the result obtaining is as shown in table 1.
After the domestication of table 1 bletilla striata tissue cultured seedling, transplant survival rate
| Group | Matrix | Survival number (strain) | Survival rate (%) |
[0044]?
| 1 | Contrast media | 40 | 80% |
| 2 | Embodiment 1 | 43 | 86% |
| 3 | Embodiment 2 | 43 | 86% |
| 4 | Embodiment 3 | 45 | 90% |
| 5 | Embodiment 4 | 44 | 88% |
| 6 | Embodiment 5 | 44 | 88% |
As shown in Table 1, the survival rate of organizing 1～organizing the bletilla striata tissue cultured seedling in 6 is all higher, illustrates that contrast media and domesticating and cultivating matrix of the present invention all have more outstanding domestication ability; But the survival rate of organizing 2～organizing bletilla striata tissue cultured seedling in 6 is higher.This may be because, contrast media is suitable for use as the domesticating and cultivating of bletilla striata bulb, and domesticating and cultivating matrix in the embodiment of the present invention 1～6 is more suitable for the domesticating and cultivating of bletilla striata tissue cultured seedling, the formula of this and matrix presents dependency; May be also because the domestication of domesticating and cultivating matrix of the present invention is shorter, and the domestication of contrast media is longer than the domestication of matrix of the present invention, and bletilla striata tissue cultured seedling has been caused to impact.In group 2～organize 6, survival rate: organize 4 > and organize 5/ group of 6 > and organize 2/ group 3, meaning is the substrate formula that the substrate formula in the embodiment of the present invention 3 is better than embodiment 4 and 5, and the substrate formula in embodiment 3,4,5 is better than the substrate formula in embodiment 1,2.Therefore, the optimum formula of bletilla striata domesticating and cultivating matrix of the present invention is: anosis loam 25%, vegetable mould 25%, perlite 50%; More excellent formula is: anosis loam 22.5%～27.5%, vegetable mould 22.5%～27.5%, perlite 45%～55%; Suboptimum formula is: anosis loam 20%～30%, vegetable mould 20%～30%, perlite 40%～60%.
Claims (5)
1. a bletilla striata tissue cultured seedling domesticating and cultivating matrix is to be formed according to following volume percent is composite by anosis loam, vegetable mould, perlite:
Anosis loam 20%～30%, vegetable mould 20%～30%, perlite 40%～60%.
2. a kind of bletilla striata tissue cultured seedling domesticating and cultivating matrix according to claim 1, is characterized in that: the volume percent of described raw material is:
Anosis loam 22.5%～27.5%, vegetable mould 22.5%～27.5%, perlite 45%～55%.
3. a kind of bletilla striata tissue cultured seedling domesticating and cultivating matrix according to claim 1, is characterized in that: the volume percent of described raw material is:
Anosis loam 25%, vegetable mould 25%, perlite 50%.
4. the preparation method of a kind of bletilla striata tissue cultured seedling domesticating and cultivating matrix according to claim 1, is characterized in that: be according to volume percent mixing and stirring by each raw material.
5. the preparation method of a kind of bletilla striata tissue cultured seedling domesticating and cultivating matrix according to claim 1, is characterized in that: be by each raw material according to volume percent mixing and stirring after, adjust EC value lower than 1.0ms/cm.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126398A (en) * | 2014-07-29 | 2014-11-05 | 郎溪县紫兰药材种植专业合作社 | Compound cultivation method for interplanting of traditional Chinese medicine bletilla striata in pine forest |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN106305055A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Domestication method for rhizoma bletillae seedlings |
| CN106396965A (en) * | 2016-09-19 | 2017-02-15 | 湖南新杰农业开发有限公司 | Nutrition soil for promoting transplanting survival of large bletilla striata seedlings and preparation method thereof |
| CN108055997A (en) * | 2017-12-05 | 2018-05-22 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of high density temporary planting method of sugar-cane tissue culture seedlings |
| CN109496782A (en) * | 2018-12-26 | 2019-03-22 | 广西壮族自治区农业科学院生物技术研究所 | A kind of temporary planting matrix and preparation method thereof being conducive to the growth of bletilla tissue-cultured seedling |
| CN114667857A (en) * | 2022-02-08 | 2022-06-28 | 贵州省农作物品种资源研究所 | Bletilla striata seedling domestication optimization method and domesticated bletilla striata seedlings |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126398A (en) * | 2014-07-29 | 2014-11-05 | 郎溪县紫兰药材种植专业合作社 | Compound cultivation method for interplanting of traditional Chinese medicine bletilla striata in pine forest |
| CN104126398B (en) * | 2014-07-29 | 2016-08-24 | 郎溪县紫兰药材种植专业合作社 | The pine forest interplanting Chinese medicine bletilla striata is combined breeding method |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN106305055A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Domestication method for rhizoma bletillae seedlings |
| CN106396965A (en) * | 2016-09-19 | 2017-02-15 | 湖南新杰农业开发有限公司 | Nutrition soil for promoting transplanting survival of large bletilla striata seedlings and preparation method thereof |
| CN108055997A (en) * | 2017-12-05 | 2018-05-22 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of high density temporary planting method of sugar-cane tissue culture seedlings |
| CN109496782A (en) * | 2018-12-26 | 2019-03-22 | 广西壮族自治区农业科学院生物技术研究所 | A kind of temporary planting matrix and preparation method thereof being conducive to the growth of bletilla tissue-cultured seedling |
| CN114667857A (en) * | 2022-02-08 | 2022-06-28 | 贵州省农作物品种资源研究所 | Bletilla striata seedling domestication optimization method and domesticated bletilla striata seedlings |
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Application publication date: 20140115 |