CN102948325A

CN102948325A - Cordyceps militaris efficient quick cultivation technology

Info

Publication number: CN102948325A
Application number: CN 201110250298
Authority: CN
Inventors: 王再飞
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-08-29
Filing date: 2011-08-29
Publication date: 2013-03-06

Abstract

The invention discloses a cordyceps militaris efficient quick cultivation technology. The raw materials of a culture medium include rice, corn, bran coat, silkworm chrysalis meal and a nutrient solution prepared from water, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, tri-ammonium citrate and vitamin B. The formula of the nutrient solution is as follows: 10g of glucose, 5g of peptone, 2g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 1g of tri-ammonium citrate and 10mg of vitamin B are added into every 1,000ml of water; and the raw materials of the culture medium include 20-25% of rice, 10-15% of corn, 4-8% of bran coat, 1-2% of silkworm chrysalis meal and 50-55% of nutrient solution by weight. The technology disclosed by the invention can regenerate the cordyceps resources on the verge of extinction so that the cordyceps can be produced in a large scale to meet the needs of people and markets; and meanwhile, thousands of farmers become rich and workers get work.

Description

The efficient quick cultivating technology of a kind of northern Chinese caterpillar fungus

Technical field

The present invention relates to a kind of Chinese caterpillar fungus quick cultivating technology, relate in particular to a kind of northern Chinese caterpillar Fungus high-yield culture technique.

Background technology

China is worm grass resources big country, and Chinese caterpillar fungus is the general designation of Cordyceps sinensis fungus, and northern Chinese caterpillar Fungus is also named Cordyceps militaris, northern Chinese caterpillar fungus.Cordyceps militaris plantation succeeds in the many areas of China, but existing culture technique output is extremely low, and existing process technology also can only be produced several medicines, and the demand of this and domestic and international market differs greatly.Always the wait in expectation cordyceps cultivation technology of a good quality and high output of people; Cordyceps militaris food, beverage, the functional food of the series that waits in expectation move towards market satisfying people to new nutraceutical, the demand of functional food, and this is insurmountable problem always for a long time.

Summary of the invention

For the defective of prior art, the object of the present invention is to provide a kind of efficient quick cultivating technology of a kind of northern Chinese caterpillar fungus fast, that survival rate is high, growth cycle is short of breeding.

To achieve these goals, the technical solution adopted in the present invention is: the efficient quick cultivating technology of a kind of northern Chinese caterpillar fungus, the raw material that adopts is rice, corn, cavings, dried silkworm chrysalis meal and by water, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, Triammonium citrate, the formulated nutrient solution of Cobastab, and the prescription of nutrient solution is: add glucose 10 grams, peptone 5 grams, potassium dihydrogen phosphate 2 grams, magnesium sulfate 1 gram, Triammonium citrate 1 gram, vitaminB10 milligram in per 1000 ml waters.Culture medium raw material composition weight proportioning is: rice 20-25%, corn 10-15%, cavings 4-8%, dried silkworm chrysalis meal 1-2%, nutrient solution 50-55%.

Breeding method of the present invention (step) is:

1, the making of medium: in container, add according to the above ratio rice, corn, cavings, dried silkworm chrysalis meal and nutrient solution, container closure, for subsequent use with cooling after the conventional method sterilization;

2, inoculation: under without the bacterium condition, operate, aweto is accessed in the medium;

3, bacteria: the container that will access bacterium places under 12.17 degrees centigrade the lucifuge environment and cultivated 20-25 days, until white hypha covers with medium more than 2/3;

4, stimulate the generation of fruit body primordium: stimulated 10 days under the condition of day and night temperature more than 10 degrees centigrade behind the annesl, media surface produces a large amount of fruit body primordiums.Day and night temperature scope: 5.25 degrees centigrade, in the sealing part perforation, make the culture space proper ventilation simultaneously, to supply with sufficient oxygen;

5, sporophore growth: under 18-25 degree centigrade, the condition of relative moisture 85.90%, the stable cultivation 20-25 days, fruit body covers with container, and length reaches 6.7cm;

6, pluck receipts: open sealing, fruit body is shoveled down gently In Shade airing or oven dry from root with the little spades with elbow;

The present invention can make and be on the verge of exhausted worm grass resources and obtain new life, thereby the urgent need in people and market is satisfied in large-scale production, can make thousands of farmer richness, workman on duty simultaneously.

Embodiment

Embodiment: the efficient quick cultivating technology of a kind of northern Chinese caterpillar fungus, medium is: rice 25%, corn 15%, cavings 8%, dried silkworm chrysalis meal 2%, nutrient solution 55%, incubation step: 1, preparation medium, 2, inoculation, 3, the bacteria environmental temperature is 17 degrees centigrade, lucifuge was cultivated 25 days, 4, annesl, 5, stimulate fruit body primordium to produce, day and night temperature 10 degrees centigrade (15 degrees centigrade of daytimes, 25 degrees centigrade of nights) stimulated 10 days, and 6, sporophore growth is 25 degrees centigrade of temperature, cultivated 7, pluck the receipts fruit body under relative moisture 90% ventilation condition 25 days.

Claims

1. efficient quick cultivating technology of northern Chinese caterpillar fungus, raw material is rice, corn, cavings, dried silkworm chrysalis meal and by water, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, Triammonium citrate, the formulated nutrient solution of Cobastab, the proportioning of nutrient solution is: add glucose 10 grams, peptone 5 grams, potassium dihydrogen phosphate 2 grams, magnesium sulfate 1 gram, Triammonium citrate 1 gram, vitaminB10 milligram in per 1000 ml waters, culture medium raw material composition weight proportioning is: rice 20-25%, corn 10-15%, cavings 4-8%, dried silkworm chrysalis meal 1-2%, nutrient solution 50-55%.