CN106912295A - A kind of method of use China fir quickly breeding Antrodia camphorata fructification - Google Patents
A kind of method of use China fir quickly breeding Antrodia camphorata fructification Download PDFInfo
- Publication number
- CN106912295A CN106912295A CN201710141697.8A CN201710141697A CN106912295A CN 106912295 A CN106912295 A CN 106912295A CN 201710141697 A CN201710141697 A CN 201710141697A CN 106912295 A CN106912295 A CN 106912295A
- Authority
- CN
- China
- Prior art keywords
- china fir
- antrodia camphorata
- quickly breeding
- days
- selection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The present invention relates to a kind of method of use China fir quickly breeding Antrodia camphorata fructification, it is material to select fresh China fir, coordinates growth nutrient solution, and through packing sterilization, inoculated and cultured can form Antrodia camphorata fruit body primordium in 90 days, be further cultured for harvest for 60 90 days.The method is with short production cycle, and materials are easy, aboundresources, and cultivation highly shortened Antrodia camphorata fructification incubation time, improve annual production, reduce production cost, increase producer's economic benefit.
Description
Technical field
The invention belongs to bacterium industry field, and in particular to a kind of method of use China fir quickly breeding Antrodia camphorata fructification.
Background technology
Antrodia camphorata also known as cinnamomum kanahirai hay mushroom, are a kind of precious medicinal fungis being grown in cinnamomum kanehirai, containing many in Antrodia camphorata
The Multiple components such as sugared body, terpenoid, Sudismase and nucleic acid, with removing toxic substances liver protection, anticancer, reinforced immunological,
The multiple efficacies such as antiallergy, reducing blood lipid, by the very big welcome of people.Antrodia camphorata bacterium is stronger to the selectivity of matrix, is one
Plant the very strong domestomycetes of typical, selectivity.The artificial cultivation method of current Antrodia camphorata is cultivated using dry wood, such as Cinnamomum kanahirai hay
Tree cultivation basswood method, incubation time are long, needs could produce fructification, the high cost of cost, the Antrodia camphorata for obtaining for more than 1 year
Yield poorly.The present invention uses fresh chinese fir culture, with the use of growth nutrient solution, when greatly shortening Antrodia camphorata fruit-body formation
Between, form Antrodia camphorata former base within 90 days or so after general inoculation, harvesting can be connect within 150-180 days, biological transformation ratio is up to 10-20%.I
State's various regions China fir aboundresources, materials are easy, using China fir quickly breeding Cinnamomum kanahirai hay massee fruiting bodies, not only improve to the wild of rareness
The protection of cinnamomum kanehirai resource, promotes the sound development of the traditional Chinese medicine enterprise of Antrodia camphorata raw material again, can bring good Ecological Effect
Should, economic benefits and social effect.
The content of the invention
It is an object of the invention to provide a kind of method of use China fir quickly breeding Antrodia camphorata fructification.The method is produced
Cycle is short, materials are easy, aboundresources, and cultivation highly shortened Antrodia camphorata fructification incubation time, improve annual production, reduce
Production cost, increases producer's economic benefit.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of method of use China fir quickly breeding Antrodia camphorata fructification, comprises the steps of：A. the selection and processing of China fir；
B. bacteria selection and inoculation；C. cultivation management：
A. the selection and processing of China fir：
The fresh China fir of selection, diameter 10-20cm, peeling is cut into 10-20cm segments long or according to the plank block into 5*10*20,
Immersion clear water 6d, changes water once, then autoclaving (121 DEG C, 10-20h) daily, and by material, cross section is stained with battalion end to end for taking-up
Nutrient solution, then packs, then autoclaving (121 DEG C of 2-4h), standby.
Nutrient solution prescription：It is Cinnamomum kanahirai hay wood powder 1.0-5.0wt%, sucrose 1.0-2.0wt%, peptone 0.1-0.3wt% to be formulated,
Ammonium citrate 0.05-0.1wt%, sterilized water 92.6-97.85wt%.Cinnamomum kanahirai hay wood powder is added after first boiling 10 minutes with sterilized water
Other medicines are nutrient solution.
B. bacteria selection and inoculation：
Screening liquid spawn culture medium formula is：Cinnamomum kanahirai hay wood powder 0.1-0.2wt%, potato starch 1.0-1.5wt%, coixlacrymajobi powder 1.0-
1.5wt%, corn flour 1.0-2.0wt%, sucrose 2.0-3.0wt%, peptone 0.1-0.3wt%, potassium dihydrogen phosphate 1.0-1.5wt%,
Magnesium sulfate 0.7-1.0wt%, sterilized water 89-93.1wt%.Using 500ml triangular flasks, per bottled 250ml culture mediums, routinely go out
Bacterium, inoculation；Distant bed shaken cultivation, amplitude 150r/min controls 26 DEG C -28 DEG C of temperature, incubation time 5-7d；Cultivation sowing quantity
Account for the 5-10wt% of matrix weight.
C. cultivation management：
Cultivated in darkroom after inoculation, 28-30 DEG C of room temperature, humidity 60%；After culture 30d, room temperature is controlled at 22 DEG C~25 DEG C, then is trained
At 10 DEG C~15 DEG C, on 20 DEG C~25 DEG C of daytime, relative air humidity is maintained at 70%~80% for nocturnal temperature control after supporting 30d；When
When red patch occurs in stromal surface, former base is initially formed, and unties packaging bag, comes mushroom producing room, and room temperature is controlled 22 DEG C~25
DEG C, relative air humidity is maintained at 90%~95%；Culture 60-90d harvestings.
The invention has the advantages that：There is provided a kind of method of use China fir quickly breeding Antrodia camphorata fructification, using fresh
China fir culture Antrodia camphorata fructification, fruiting morning, annual production is high；Abundant raw material, source is easy, cultivates low cost, economic benefit
Significantly.
Specific embodiment
For further disclosure is of the invention rather than limitation, below in conjunction with example, the present invention is described in further detail.
Embodiment 1
A kind of method of use China fir quickly breeding Antrodia camphorata fructification, comprises the following steps that：
A. the selection and processing of China fir；B. bacteria selection and inoculation；C. cultivation management：
A. the selection and processing of China fir：
20cm segments long are cut into the fresh China fir of selection, diameter 20cm, peeling, soak clear water 6d, water are changed daily once, so
Autoclaving (121 DEG C, 20h) afterwards, take out that cross section is stained with nutrient solution end to end by material, then pack, then autoclaving (121
DEG C 4h), it is standby.
Nutrient solution prescription：Be formulated is Cinnamomum kanahirai hay wood powder 2.0wt%, sucrose 1.0wt%, peptone 0.1wt%, ammonium citrate
0.1wt%, sterilized water 96.8wt%.Cinnamomum kanahirai hay wood powder adds other medicines as nutrient solution after first being boiled 10 minutes with sterilized water.
B. bacteria selection and inoculation：
Screening liquid spawn culture medium formula is：Cinnamomum kanahirai hay wood powder 0.2wt%, potato starch 1.5wt%, coixlacrymajobi powder 1.0wt%, corn
Powder 1.5wt%, sucrose 3.0wt%, peptone 0.2wt%, potassium dihydrogen phosphate 1.5wt%, magnesium sulfate 1.0wt%, sterilized water 96.8wt%；
Using 500ml triangular flasks, per bottled 250ml culture mediums, routinely sterilize, be inoculated with；Distant bed shaken cultivation, amplitude 150r/min,
26 DEG C of temperature of control, incubation time 7d；Every bag of China fir meets bacterium culture medium bacterium solution 70ml.
C. cultivation management：
Cultivated in darkroom after inoculation, 28 DEG C of room temperature, humidity 60%；After culture 30d, room temperature is controlled at 25 DEG C, is further cultured for night after 30d
Between temperature control at 15 DEG C, on 25 DEG C of daytime, relative air humidity is maintained at 75%；When there is red patch in stromal surface, former base
Initially form, untie packaging bag, come mushroom producing room, room temperature is controlled at 24 DEG C, and relative air humidity is maintained at 90%~95%；Culture
60-90 d are harvested.
Embodiment 2
A kind of method of use China fir quickly breeding Antrodia camphorata fructification, comprises the following steps that：
A. the selection and processing of China fir：
The fresh China fir of selection, according to the plank block into 5*10*20, soaks clear water 6d, water is changed daily once, then autoclaving
(121 DEG C, 10h), by material, cross section is stained with nutrient solution end to end for taking-up, then packs, then autoclaving (121 DEG C of 2h), standby.
Nutrient solution prescription：Be formulated is Cinnamomum kanahirai hay wood powder 5.0wt%, sucrose 2.0wt%, peptone 0.1wt%, ammonium citrate
0.1wt%, sterilized water 96.8wt%.Cinnamomum kanahirai hay wood powder adds other medicines as nutrient solution after first being boiled 10 minutes with sterilized water.
B. bacteria selection and inoculation：
Screening liquid spawn culture medium formula is：Cinnamomum kanahirai hay wood powder 0.2wt%, potato starch 1.5wt%, coixlacrymajobi powder 1.0wt%, corn
Powder 1.5wt%, sucrose 3.0wt%, peptone 0.2wt%, potassium dihydrogen phosphate 1.5wt%, magnesium sulfate 1.0wt%, sterilized water 90.1wt%；
Using 500ml triangular flasks, per bottled 250ml culture mediums, routinely sterilize, be inoculated with；Distant bed shaken cultivation, amplitude 150r/min,
26 DEG C of temperature of control, incubation time 7d；Every bag of Chinese fir board meets bacterium culture medium bacterium solution 50ml.
C. cultivation management：
Cultivated in darkroom after inoculation, 30 DEG C of room temperature, humidity 60%；After culture 30d, room temperature is controlled at 25 DEG C, is further cultured for night after 30d
Between temperature control at 15 DEG C, on 25 DEG C of daytime, relative air humidity is maintained at 75%；When there is red patch in stromal surface, former base
Initially form, untie packaging bag, come mushroom producing room, room temperature is controlled at 24 DEG C, and relative air humidity is maintained at 90%~95%；Culture
60-90d is harvested.
Embodiment 3
A kind of method of use China fir quickly breeding Antrodia camphorata fructification, comprises the following steps that：
A. the selection and processing of China fir：
The fresh China fir of selection, according to the plank block into 5*10*20, soaks clear water 6d, water is changed daily once, then autoclaving
(121 DEG C, 10h), by material, cross section is stained with nutrient solution end to end for taking-up, then packs, then autoclaving (121 DEG C of 2h), standby.
Nutrient solution prescription：Be formulated is Cinnamomum kanahirai hay wood powder 3.0wt%, sucrose 2.0wt%, peptone 0.3wt%, ammonium citrate
0.1wt%, sterilized water 96.6wt%.Cinnamomum kanahirai hay wood powder adds other medicines as nutrient solution after first being boiled 10 minutes with sterilized water.
B. bacteria selection and inoculation：
Screening liquid spawn culture medium formula is：Cinnamomum kanahirai hay wood powder 0.1wt%, potato starch 1.5wt%, coixlacrymajobi powder 1.5wt%, corn
Powder 1.5wt%, sucrose 2.0wt%, peptone 0.1wt%, potassium dihydrogen phosphate 1.5wt%, magnesium sulfate 1.0wt%, sterilized water 90.8wt%；
Using 500ml triangular flasks, per bottled 250ml culture mediums, routinely sterilize, be inoculated with；Distant bed shaken cultivation, amplitude 150r/min,
26 DEG C of temperature of control, incubation time 7d；Every bag of Chinese fir board meets bacterium culture medium bacterium solution 50ml.
C. cultivation management：
Cultivated in darkroom after inoculation, 30 DEG C of room temperature, humidity 60%；After culture 30d, room temperature is controlled at 25 DEG C, is further cultured for night after 30d
Between temperature control at 15 DEG C, on 25 DEG C of daytime, relative air humidity is maintained at 75%；When there is red patch in stromal surface, former base
Initially form, untie packaging bag, come mushroom producing room, room temperature is controlled at 24 DEG C, and relative air humidity is maintained at 90%~95%；Culture
60-90d is harvested.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to covering scope of the invention.
Claims (5)
1. a kind of method of use China fir quickly breeding Antrodia camphorata fructification, it is characterised in that：Comprise the steps of：A. China fir
Selection and processing；B. bacteria selection and inoculation；C. cultivation management.
2. the method for a kind of use China fir quickly breeding Antrodia camphorata fructification according to claim 1, it is characterised in that：Institute
State the selection and processing of China fir：The fresh China fir of selection, diameter 10-20cm, peeling is cut into 10-20cm segments long or is sawn into
The plank block of 5*10*20, immersion clear water 6 days, changes water once, then the autoclaving 10-20h at 121 DEG C daily, and taking out will
Cross section is stained with nutrient solution to material end to end, then packs, then the autoclaving 2-4 h at 121 DEG C, standby.
3. the method for a kind of use China fir quickly breeding Antrodia camphorata fructification according to claim 2, it is characterised in that：Institute
Nutrient solution is stated to be made up of following raw material：Cinnamomum kanahirai hay wood powder 1.0-5.0wt%, sucrose 1.0-2.0wt%, peptone 0.1-0.3wt%, lemon
Lemon acid ammonium 0.05-0.1wt% and sterilized water 92.6-97.85wt%；Wherein Cinnamomum kanahirai hay wood powder is first boiled before nutrient solution is prepared with sterilized water
Open 10 minutes.
4. the method for a kind of use China fir quickly breeding Antrodia camphorata fructification according to claim 1, it is characterised in that：Institute
Bacteria selection is stated with inoculation：Using 500ml triangular flasks, per bottled 250ml culture mediums, routinely sterilize, be inoculated with, distant bed vibration
Culture, amplitude 150r/min controls 26 DEG C -28 DEG C of temperature, and incubation time 5-7 days, cultivation sowing quantity accounts for the 5- of matrix weight
10wt%；The culture medium is made up of following raw material：Cinnamomum kanahirai hay wood powder 0.1-0.2wt%, potato starch 1.0-1.5wt%, coixlacrymajobi powder
1.0-1.5wt%, corn flour 1.0-2.0wt%, sucrose 2.0-3.0wt%, peptone 0.1-0.3wt%, potassium dihydrogen phosphate 1.0-
1.5wt%, magnesium sulfate 0.7-1.0wt% and sterilized water 89-93.1wt%.
5. the method for a kind of use China fir quickly breeding Antrodia camphorata fructification according to claim 1, it is characterised in that：Institute
The cultivation management stated：Cultivated in darkroom after inoculation, 28-30 DEG C of room temperature, humidity 60%；Culture 30 days after, room temperature control 22 DEG C~
25 DEG C, at 10 DEG C~15 DEG C, on 20 DEG C~25 DEG C of daytime, relative air humidity is maintained at for nocturnal temperature control after being further cultured for 30 days
70%~80%；When red patch occurs in stromal surface, former base is initially formed, and unties packaging bag, comes mushroom producing room, room temperature control
At 22 DEG C~25 DEG C, relative air humidity is maintained at 90%~95%；Culture is harvested for 60-90 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710141697.8A CN106912295A (en) | 2017-03-10 | 2017-03-10 | A kind of method of use China fir quickly breeding Antrodia camphorata fructification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710141697.8A CN106912295A (en) | 2017-03-10 | 2017-03-10 | A kind of method of use China fir quickly breeding Antrodia camphorata fructification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106912295A true CN106912295A (en) | 2017-07-04 |
Family
ID=59460751
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710141697.8A Pending CN106912295A (en) | 2017-03-10 | 2017-03-10 | A kind of method of use China fir quickly breeding Antrodia camphorata fructification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106912295A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235698A (en) * | 2019-07-23 | 2019-09-17 | 漳州帝宝牛樟芝生物科技股份有限公司 | A kind of cultural method of Antrodia camphorata |
| CN115152529A (en) * | 2022-08-18 | 2022-10-11 | 夏兴林 | Planting and cultivating method of ganoderma lucidum |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191120B (en) * | 2006-11-28 | 2010-05-19 | 蔡达铭 | Culture method for antrodia cinnamomea |
| CN104541977A (en) * | 2015-01-08 | 2015-04-29 | 福建农林大学 | Chinese fir board cultivating method of antrodia cinnamomea |
| CN104756754A (en) * | 2015-02-03 | 2015-07-08 | 上海勤生缘生物科技有限公司 | Method for cultivating Antrodia camphorata body |
| CN105052558A (en) * | 2015-09-08 | 2015-11-18 | 福建农林大学 | Antrodia cinnamomea quick cultivation method |
-
2017
- 2017-03-10 CN CN201710141697.8A patent/CN106912295A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191120B (en) * | 2006-11-28 | 2010-05-19 | 蔡达铭 | Culture method for antrodia cinnamomea |
| CN104541977A (en) * | 2015-01-08 | 2015-04-29 | 福建农林大学 | Chinese fir board cultivating method of antrodia cinnamomea |
| CN104756754A (en) * | 2015-02-03 | 2015-07-08 | 上海勤生缘生物科技有限公司 | Method for cultivating Antrodia camphorata body |
| CN105052558A (en) * | 2015-09-08 | 2015-11-18 | 福建农林大学 | Antrodia cinnamomea quick cultivation method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235698A (en) * | 2019-07-23 | 2019-09-17 | 漳州帝宝牛樟芝生物科技股份有限公司 | A kind of cultural method of Antrodia camphorata |
| CN115152529A (en) * | 2022-08-18 | 2022-10-11 | 夏兴林 | Planting and cultivating method of ganoderma lucidum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103598010B (en) | Original ecological imitative wild cultivation method for inonotus sanghuang | |
| CN102786333A (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
| CN102301912B (en) | Method for culturing Cordyceps militaris by using highland barley as main raw material | |
| CN103250529B (en) | Method of cultivating chrysanthemum by probiotic nutrients | |
| CN101720627A (en) | Method for culturing cordyceps militaris by living silkworm chrysalises | |
| CN101816256A (en) | Method for cultivating microbial strain of phellinus linteus and planting phellinus linteus | |
| CN102224792B (en) | Aquaculture method of selenium-rich hericium erinaceus | |
| CN105052558A (en) | Antrodia cinnamomea quick cultivation method | |
| CN102440148A (en) | Method for industrially planting pleurotus eryngii by utilizing mulberry wood chips | |
| CN102154407A (en) | Corayceps militaris polysaccharide two-stage fermentation synthesis process | |
| CN105706732A (en) | Method for cultivating ganoderma applanatum | |
| CN104541977B (en) | A kind of Chinese fir board culture method of Antrodia camphorata | |
| CN104335820A (en) | Production method of gastrodia elata associated honey fungus strain | |
| CN102948325A (en) | Cordyceps militaris efficient quick cultivation technology | |
| CN102812847A (en) | Cultivating method of pleurotus nebrodensis | |
| CN102318544A (en) | A kind of Cordyceps militaris ciltivating process | |
| CN107333568A (en) | A kind of more batches of culture techniques of Cordyceps militaris constant temperature | |
| CN102318545B (en) | Water culturing method for hericium erinaceus | |
| CN106912295A (en) | A kind of method of use China fir quickly breeding Antrodia camphorata fructification | |
| CN102027859A (en) | Process for culturing mushrooms with wine aroma flavor | |
| CN105255744B (en) | A kind of lucidum strain purification and rejuvenation cultural method and its culture medium | |
| CN103270890A (en) | Method of culturing Taiwanofungus camphoratus by dish | |
| CN103858659A (en) | Bag cultivation method of black fungi | |
| CN103449896B (en) | A kind of culture material of Xinbao mushroom culturing and preparation method | |
| CN107926482A (en) | A kind of method using fluid nutrient medium production fruiting bodies of cordyceps militaris |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170704 |