CN102224792B - Aquaculture method of selenium-rich hericium erinaceus - Google Patents
Aquaculture method of selenium-rich hericium erinaceus Download PDFInfo
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Abstract
The invention discloses an aquaculture method of selenium-rich hericium erinaceus and belongs to the production field of edible fungi. The is characterized in that a selenium-rich hericium erinaceus culture medium is a nutritive liquid which contains sodium selenite. The aquaculture method mainly comprises the following steps: (1) preparing a strain; (2) selecting a wide-mouth bottle as an aquaculture container; (3) preparing the aquaculture nutritive liquid by utilizing 0.05g of sodium selenite, 20g of glucose, 8g of peptone, 3g of magnesium sulfate, 3g of monopotassium phosphate and 0.1g of vitamin B; (4) sterilizing and inoculating; and (5) culturing and managing. The method provided by the invention has the advantages of simplicity in operation, less investment and low cost; the production period is 1/2-1/3 of that based on a solid culture media; and the method is suitable for industrial production and wide in application prospect. The content of the selenium in the per kilogram of the dried hericium erinaceus product produced by the method can reach 0.3-0.5 milligram, wherein the contents of the organic selenide which can be easily absorbed by a human body is 65-85% which is far higher than the selenium content indicator in the national standard of the selenium-rich rice.
Description
Technical field
The present invention relates to a kind of cultivating method for mushroom, particularly relate to the ciltivating process of a kind of rich selenium Hericium erinaceus, belong to the Edible Fungi field.
Background technology
Selenium is trace element essential among the human life activity, and the laudatory title of " kindling material of life " is arranged, though content is little in human body, it is to the regulation and control of human physiological functions and keep the great role of human body best nutritional state.Selenium have remove free radical, anti-ageing, improve function such as immunity.Human body mainly obtains from food the absorption of selenium, and the Se content in the food has determined to mend the effect of selenium, and occurring in nature, some biology have selenium-rich function, can improve the Se content of food.Experiment showed, that in a large number some edible mushrooms have selenium-rich function, Hericium erinaceus is exactly one of rich selenium mushroom.(paper of the He Donglan that delivers referring to " hubei agricultural science " 2005 the 4th phases " influences of hericium mycelium growths such as sodium selenite ", the paper " comparisons of four kinds of edible mushroom selenium rich abilities " of the Shang Dejing that 1999 the 3rd phases " edible mushroom journal " deliver).
Hericium erinaceus is the famous food medicine dual-purpose fungi of China, is called " king of mushroom " usually, and it and bear's paw, bird's nest, shark's fin are listed as four big famous dishes, are described as " mountain delicacy " since ancient times.
Hericium erinaceus belongs to wood decay fungi, and wild Hericium erinaceus generally is grown on the withered tree, and the artificial cultivation Hericium erinaceus is mainly cultivated with solid materials such as wood chip, cotton seed hulls, corncobs, generally adopts horizontal being thrown on mushroom bed or the mushroom frame of bacterium bag to carry out field production.Chinese patent CN 101803536 A disclose a kind of field production Hericium erinaceus method; Chinese patent CN 101919338 A disclose a kind of industrialized cultivation method for hericium, are primary raw material with corncob, wood chip, cotton seed hulls, adopt the batch production production technology to carry out the Hericium erinaceus cultivation.In the prior art, the cultivation matrix of Hericium erinaceus all is a solid culture medium, applicant autumn in 2010; In the process of carrying out the edible fungi liquid strain test, chance on the Hericium erinaceus liquid spawn of inoculation, after putting a period of time; The surface of liquid spawn has grown hericium erinaceus fruiting body unexpectedly, and unexpected the discovery excited applicant's research enthusiasm; Spring in 2011, the applicant carries out the test of Hericium erinaceus liquid strain cultivation specially, and the result has grown Hericium erinaceus for every bottle.The characteristic that has selenium-rich function according to Hericium erinaceus; The applicant has carried out rich selenium Hericium erinaceus water culture experiment again; In mill water culture nutrient solution, add sodium selenite, make that Se content reaches the 30-50 microgram in the 100 gram fresh Hericium erinaceus fruit bodys, wherein human body organic selenides of being easy to absorb accounts for 65-85%.
Summary of the invention
The ciltivating process that the purpose of this invention is to provide a kind of rich selenium Hericium erinaceus.
Technical scheme of the present invention realizes in the following manner:
The ciltivating process of a kind of rich selenium Hericium erinaceus is characterized in that: the cultivation matrix of rich selenium Hericium erinaceus is a nutrient solution, contains sodium selenite in the said nutrient solution; Said ciltivating process mainly comprises following steps:
1, the preparation of rich selenium hedgehog fungus bacterial
1) the female kind: in the PDA of every 1000ml medium, add the sodium selenite of 50-100mg, cultivate processing absorption and the conversion capability of hericium mycelium initial stage to selenium.
2) Hericium erinaceus produces kind of an employing liquid spawn, and the liquid spawn culture medium prescription is: contain sodium selenite 100-200mg, Cobastab in every 1000ml culture fluid
150-200mg, peptone 5-8g, glucose 15-20g, magnesium sulfate 1-3g, potassium dihydrogen phosphate 1-3g; Medium pH value 6.5-7.5.
2, rich selenium Hericium erinaceus water planting container selection
Select wide-mouth bottle as rich selenium Hericium erinaceus water planting container, be convenient to the harvesting of hericium erinaceus fruiting body.
3, the preparation of rich selenium Hericium erinaceus mill water culture nutrient solution
The prescription of rich selenium Hericium erinaceus mill water culture nutrient solution is: contain potato 100-200g, wheat bran 50-100g, rice bran 50-100g, corn flour 50-100g, analysis for soybean powder 50-100g, cotton seed hulls 50-500g in every 1000ml nutrient solution; Also contain sodium selenite 0.2-0.5g, glucose 15-20g, peptone 6-8g, magnesium sulfate 2-4g, potassium dihydrogen phosphate 2-4g, Cobastab 0.05-0.2g.
The compound method of rich selenium Hericium erinaceus mill water culture nutrient solution is: the quantity of preparing nutrient solution as required; Take by weighing potato, wheat bran, rice bran, corn flour, analysis for soybean powder, cotton seed hulls in proportion respectively, earlier the potato decortication stripping and slicing is poured in the pot, again other raw materials are poured in the pot; After adding the water stirring; Slow fire boiled 20-30 minute, and 8 layers of filtered through gauze are extracted its juice, and filtrating adds water and supplies required preparation quantity; Take by weighing sodium selenite, glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, Cobastab more in proportion; The filtrating of supplying water was simmered in water 3-10 minute again, and boiled back adds sodium selenite, glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, vitamin, treats that all nutriments all get final product after the dissolving.
4, dress liquid sterilization
The Hericium erinaceus mill water culture nutrient solution for preparing in the step 3) is respectively charged in the water planting container, carries out sterilization treatment behind the container closure.
5, inoculation
To pass through the step 4) sterilization treatment, have the rich selenium Hericium erinaceus water planting container of nutrient solution, put into superclean bench or inoculating hood, in gnotobasis just in the step 1) cultured Hericium erinaceus liquid spawn insert in the water planting container, inoculum concentration is 10-15%.
6, cultivate management
Step 5) is connected the rich selenium Hericium erinaceus water planting container of bacterial classification, put into 25-27.5 ℃ culturing room and send out bacterium and cultivated 7-15 days; In 1-7 days, rock 1-2 every day, leaves standstill after 7 days to cultivate 3-5 days; Water planting container nutrient solution surface will grow a large amount of aerial hyphaes, and the white particle of grain of rice shape occurs, suitably give illumination after white particle will develop into hericium erinaceus fruiting body; Can gather in sporophore growth 7-10 days; This fruit body is to utilize the selenium-rich function of hericium mycelium to obtain, and Se content reaches the 30-50 microgram in the 100 gram fresh Hericium erinaceus fruit bodys, and wherein human body organic selenides of being easy to absorb accounts for 65-85%.
The cultivation temperature of said Hericium erinaceus liquid spawn is 23-26 ℃, and cell age is 5-8 days, is no more than 10 days.
Said water planting container wide-mouth bottle is Cans, Clear glass bottles and jars or non-toxic transparent plastic bottle; Its volume is 250-1000ml, and bore is 50-150mm.
PH after the said rich selenium Hericium erinaceus mill water culture nutrient solution sterilization is 5.5-6.5.
The charge weight of said water planting container nutrient solution is the 1/3-1/2 of vessel volume.
Said container closure material is brown paper or gauze, is convenient to air exchange.
Beneficial effect of the present invention:
1, the rich selenium Hericium erinaceus ciltivating process of the present invention's original creation is easy and simple to handle, small investment, and cost is low.
2, water culture is sent out bacterium time much shorter than solid culture medium, and its production cycle has only the 1/2-1/3 of solid culture medium, is convenient to carry out suitability for industrialized production.
3, can change the traditional concept that wood decay fungi must rely on wooden medium growth.
4, pass through biological transformation and the selenium-rich function of Hericium erinaceus; The hericium erinaceus fruiting body of the present invention's cultivation; The per kilogram Hericium erinaceus does that Se content reaches the 0.3-0.5 milligram in the fruit body, and wherein human body organic selenides of being easy to absorb accounts for 65-85%, is higher than the Se content index of selenium-rich rice national standard far away.
Embodiment
Below in conjunction with specific embodiment; Further illustrate the present invention; Should understand this embodiment only be used to the present invention is described and be not used in the restriction scope of the present invention; After having read the present invention, those skilled in the art all fall within the application's accompanying claims institute restricted portion to the modification of the various equivalent form of values of the present invention.
Embodiment 1: a kind of rich selenium Hericium erinaceus ciltivating process mainly comprises following steps:
1, the preparation of rich selenium hedgehog fungus bacterial
A. female kind the: in the PDA of every 1000ml medium, add 100 milligrams sodium selenite, cultivate the processing absorption and the conversion capability of hericium mycelium initial stage to selenium.
B. Hericium erinaceus produces kind of an employing liquid spawn, and the liquid spawn culture medium prescription is: contain sodium selenite 150mg, Cobastab in every 1000ml culture fluid
150mg, peptone 8g, glucose 20g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g; Medium pH value 6.5-7.5.
2, rich selenium Hericium erinaceus water planting container selection
The selection volume be 500ml, bore be the wide-mouth bottle of 750mm as rich selenium Hericium erinaceus water planting container, be convenient to the harvesting of hericium erinaceus fruiting body.
3, the preparation of rich selenium Hericium erinaceus mill water culture nutrient solution
The prescription of rich selenium Hericium erinaceus mill water culture nutrient solution is: contain potato 200g, wheat bran 100g, rice bran 50g, corn flour 100g, analysis for soybean powder 50g, cotton seed hulls 200g in every 1000ml nutrient solution; Also contain sodium selenite 0.5g, glucose 20g, peptone 8g, magnesium sulfate 3g, potassium dihydrogen phosphate 3g, Cobastab 0.1g.
The compound method of rich selenium Hericium erinaceus mill water culture nutrient solution is: the quantity of preparing nutrient solution as required; Take by weighing potato, wheat bran, rice bran, corn flour, analysis for soybean powder, cotton seed hulls in proportion respectively, earlier the potato decortication stripping and slicing is poured in the pot, again other raw materials are poured in the pot; After adding the water stirring; Slow fire boiled 20-30 minute, and 8 layers of filtered through gauze are extracted its juice, and filtrating adds water and supplies required preparation quantity; Take by weighing sodium selenite, glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, Cobastab more in proportion; The filtrating of supplying water is simmered in water about 5 minutes again, and boiled back adds sodium selenite, glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, vitamin, treats that all nutriments all get final product after the dissolving.
4, dress liquid sterilization: the rich selenium Hericium erinaceus mill water culture nutrient solution for preparing in the step 3) is respectively charged in the water planting container, and the charge weight of nutrient solution is 1/2 of a vessel volume, and container carries out sterilization treatment after adopting brown paper or gauze to seal, general 1.1kg/cm
2Kept about 1 hour; PH after the mill water culture nutrient solution sterilization is 5.5-6.5.
5, inoculation: will pass through the step 4) sterilization treatment, have the rich selenium Hericium erinaceus water planting container of nutrient solution; Put into superclean bench or inoculating hood; In gnotobasis just in the step 1) cultured Hericium erinaceus liquid spawn insert in the water planting container, inoculum concentration is 10%.
6, cultivate management: step 5) is connected the rich selenium Hericium erinaceus water planting container of bacterial classification, and bacterium cultivation 7-15 days are sent out by the culturing room that puts into about 25 ℃; In 1-7 days, rock 1-2 every day, leaves standstill after 7 days to cultivate 3-5 days; Water planting container nutrient solution surface will grow a large amount of aerial hyphaes; And the white particle of grain of rice shape appears, suitably give illumination after white particle will develop into hericium erinaceus fruiting body, can gather in sporophore growth 7-10 days.
Claims (6)
1. the ciltivating process of a rich selenium Hericium erinaceus, it is characterized in that: the cultivation matrix of rich selenium Hericium erinaceus is a nutrient solution, contains sodium selenite in the said nutrient solution; Said ciltivating process mainly comprises following steps:
1) preparation of rich selenium hedgehog fungus bacterial
A. female kind the: in the PDA of every 1000ml medium, add the sodium selenite of 50-100mg, cultivate the processing absorption and the conversion capability of hericium mycelium initial stage to selenium;
B. Hericium erinaceus produces kind of an employing liquid spawn, and the liquid spawn culture medium prescription is: contain sodium selenite 100-200mg, Cobastab in every 1000ml culture fluid
150-200mg, peptone 5-8g, glucose 15-20g, magnesium sulfate 1-3g, potassium dihydrogen phosphate 1-3g; Medium pH value 6.5-7.5;
2) rich selenium Hericium erinaceus water planting container selection
Select wide-mouth bottle as rich selenium Hericium erinaceus water planting container, be convenient to the harvesting of hericium erinaceus fruiting body;
3) preparation of rich selenium Hericium erinaceus mill water culture nutrient solution
The prescription of rich selenium Hericium erinaceus mill water culture nutrient solution is: contain potato 100-200g, wheat bran 50-100g, rice bran 50-100g, corn flour 50-100g, analysis for soybean powder 50-100g, cotton seed hulls 50-500g in every 1000ml nutrient solution; Also contain sodium selenite 0.2-0.5g, glucose 15-20g, peptone 6-8g, magnesium sulfate 2-4g, potassium dihydrogen phosphate 2-4g, Cobastab 0.05-0.2g;
The compound method of rich selenium Hericium erinaceus mill water culture nutrient solution is: the quantity of preparing nutrient solution as required; Take by weighing potato, wheat bran, rice bran, corn flour, analysis for soybean powder, cotton seed hulls in proportion respectively, earlier the potato decortication stripping and slicing is poured in the pot, again other raw materials are poured in the pot; After adding the water stirring; Slow fire boiled 20-30 minute, and 8 layers of filtered through gauze are extracted its juice, and filtrating adds water and supplies required preparation quantity; Take by weighing sodium selenite, glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, Cobastab more in proportion; The filtrating of supplying water was simmered in water 3-10 minute again, and boiled back adds sodium selenite, glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, vitamin, treats that all nutriments all get final product after the dissolving;
4) dress liquid sterilization
The Hericium erinaceus mill water culture nutrient solution for preparing in the step 3) is respectively charged in the water planting container, carries out sterilization treatment behind the container closure;
5) inoculation
To pass through the step 4) sterilization treatment, have the rich selenium Hericium erinaceus water planting container of nutrient solution, and put into superclean bench or inoculating hood, and in gnotobasis, cultured Hericium erinaceus liquid spawn in the step 1) inserted in the water planting container, inoculum concentration is 10-15%;
6) cultivate management
Step 5) is connected the rich selenium Hericium erinaceus water planting container of bacterial classification, put into 25-27.5 ℃ culturing room and send out bacterium and cultivated 7-15 days; In 1-7 days, rock 1-2 every day, leaves standstill after 7 days to cultivate 3-5 days; Water planting container nutrient solution surface will grow a large amount of aerial hyphaes, and the white particle of grain of rice shape occurs, suitably give illumination after white particle will develop into hericium erinaceus fruiting body; Can gather in sporophore growth 7-10 days; This fruit body is to utilize the selenium-rich function of hericium mycelium to obtain, and Se content reaches the 30-50 microgram in the 100 gram fresh Hericium erinaceus fruit bodys, and wherein human body organic selenides of being easy to absorb accounts for 65-85%.
2. according to the ciltivating process of the said a kind of rich selenium Hericium erinaceus of claim 1, it is characterized in that: the cultivation temperature of said Hericium erinaceus liquid spawn is 23-26 ℃, and cell age is 5-8 days.
3. according to the ciltivating process of the said a kind of rich selenium Hericium erinaceus of claim 1, it is characterized in that: said water planting container wide-mouth bottle is Cans, Clear glass bottles and jars or non-toxic transparent plastic bottle; Its volume is 250-1000ml, and bore is 50-150mm.
4. according to the ciltivating process of the said a kind of rich selenium Hericium erinaceus of claim 1, it is characterized in that: the pH after the said rich selenium Hericium erinaceus mill water culture nutrient solution sterilization is 5.5-6.5.
5. according to the ciltivating process of the said a kind of rich selenium Hericium erinaceus of claim 1, it is characterized in that: the charge weight of said water planting container nutrient solution is the 1/3-1/2 of vessel volume.
6. according to the ciltivating process of the said a kind of rich selenium Hericium erinaceus of claim 1, it is characterized in that: said container closure material is brown paper or gauze, is convenient to air exchange.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102835251B (en) * | 2012-09-11 | 2013-12-11 | 史长山 | Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid |
| CN103755417B (en) * | 2013-12-27 | 2015-09-30 | 内蒙古科技大学 | A kind of production method of Hericium erinaceus (Bull. Ex Fr.) Pers. Rich in selenium mycelium liquid fermentation |
| CN105165407A (en) * | 2015-10-20 | 2015-12-23 | 上海智农生物科技有限公司 | Quantitative selenium added edible fungus liquid strain preparation mehtod and quantitative organic selenium edible fungus cultivation method |
| CN107739230B (en) * | 2017-11-24 | 2020-06-23 | 上海市农业科学院 | Method for improving hericium erinaceus polysaccharide yield and enhancing activity of macrophage in producing NO |
| CN108012758A (en) * | 2017-12-05 | 2018-05-11 | 罗成喜 | A kind of cultural method of Hericium erinaceus |
| CN108142205A (en) * | 2017-12-28 | 2018-06-12 | 江西省森旺现代农业生态科技开发有限公司 | A kind of selenium-enriched hericium erinaceus breeding method |
| CN108684439A (en) * | 2018-05-22 | 2018-10-23 | 福建农林大学 | A kind of culture medium of cracking rice cultivates method and the application of Hericium erinaceus |
| CN117084123B (en) * | 2023-10-19 | 2024-01-02 | 中国农业科学院都市农业研究所 | Water culture hericium erinaceus cultivation method |
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Address after: 212364 Danyang Jiangnan Town, Jiangsu City, the Yangtze River in Jiangsu Jiangnan Biotechnology Co., Ltd. Patentee after: Zhenjiang City Edible Fungus Research Institute Address before: 212008 Jiangsu city of Zhenjiang province Zongze Road No. 118 Zhenjiang Institute of edible fungi Patentee before: Zhenjiang City Edible Fungus Research Institute |
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Address after: 212300 Danyang hi tech Pioneer Park, South Third Ring Road, Danyang City, Zhenjiang City, Jiangsu Province Patentee after: Zhenjiang Sunong Institute of edible fungi Address before: 212364, Jiangsu City, Danyang Province Huang Tang Jiang Jiangnan Jiangsu Biotechnology Co., Ltd. Patentee before: ZHENJIANG EDIBLE FUNGUS Research Institute |
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Effective date of registration: 20211118 Address after: 212300 beside 312 National Road, Jiangshu Town, huangtang Town, Danyang City, Zhenjiang City, Jiangsu Province Patentee after: JIANGSU JIANGNAN BIOTECH CO.,LTD. Address before: 212300 Danyang hi tech Pioneer Park, South Third Ring Road, Danyang City, Zhenjiang City, Jiangsu Province Patentee before: Zhenjiang Sunong Institute of edible fungi |