CN117084123B - Water culture hericium erinaceus cultivation method - Google Patents
Water culture hericium erinaceus cultivation method Download PDFInfo
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- 240000000588 Hericium erinaceus Species 0.000 title claims abstract description 104
- 235000007328 Hericium erinaceus Nutrition 0.000 title claims abstract description 104
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 82
- 238000012364 cultivation method Methods 0.000 title claims abstract description 11
- 235000015097 nutrients Nutrition 0.000 claims abstract description 39
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 11
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 11
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 11
- 239000011781 sodium selenite Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 40
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 20
- 239000001888 Peptone Substances 0.000 claims description 20
- 108010080698 Peptones Proteins 0.000 claims description 20
- 239000008103 glucose Substances 0.000 claims description 20
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 20
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 20
- 235000019319 peptone Nutrition 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- 239000013207 UiO-66 Substances 0.000 claims description 16
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 16
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 8
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 8
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 8
- 235000001727 glucose Nutrition 0.000 claims description 8
- 229940099596 manganese sulfate Drugs 0.000 claims description 8
- 235000007079 manganese sulphate Nutrition 0.000 claims description 8
- 239000011702 manganese sulphate Substances 0.000 claims description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 8
- 229960001763 zinc sulfate Drugs 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 229930003270 Vitamin B Natural products 0.000 claims description 6
- 229930003451 Vitamin B1 Natural products 0.000 claims description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 229960003495 thiamine Drugs 0.000 claims description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 6
- 235000019156 vitamin B Nutrition 0.000 claims description 6
- 239000011720 vitamin B Substances 0.000 claims description 6
- 239000011691 vitamin B1 Substances 0.000 claims description 6
- 235000010374 vitamin B1 Nutrition 0.000 claims description 6
- 238000010189 synthetic method Methods 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 235000013877 carbamide Nutrition 0.000 claims description 2
- 229960000355 copper sulfate Drugs 0.000 claims description 2
- 229960003390 magnesium sulfate Drugs 0.000 claims description 2
- 229910007932 ZrCl4 Inorganic materials 0.000 claims 1
- DUNKXUFBGCUVQW-UHFFFAOYSA-J zirconium tetrachloride Chemical compound Cl[Zr](Cl)(Cl)Cl DUNKXUFBGCUVQW-UHFFFAOYSA-J 0.000 claims 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract description 22
- 239000011669 selenium Substances 0.000 abstract description 22
- 229910052711 selenium Inorganic materials 0.000 abstract description 22
- 229940091258 selenium supplement Drugs 0.000 abstract description 22
- 241000233866 Fungi Species 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 57
- 230000000052 comparative effect Effects 0.000 description 7
- 239000011259 mixed solution Substances 0.000 description 6
- 239000013208 UiO-67 Substances 0.000 description 5
- 229910007926 ZrCl Inorganic materials 0.000 description 4
- 239000003446 ligand Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012621 metal-organic framework Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- LFBALUPVVFCEPA-UHFFFAOYSA-N 4-(3,4-dicarboxyphenyl)phthalic acid Chemical compound C1=C(C(O)=O)C(C(=O)O)=CC=C1C1=CC=C(C(O)=O)C(C(O)=O)=C1 LFBALUPVVFCEPA-UHFFFAOYSA-N 0.000 description 1
- FZTIWOBQQYPTCJ-UHFFFAOYSA-N 4-[4-(4-carboxyphenyl)phenyl]benzoic acid Chemical compound C1=CC(C(=O)O)=CC=C1C1=CC=C(C=2C=CC(=CC=2)C(O)=O)C=C1 FZTIWOBQQYPTCJ-UHFFFAOYSA-N 0.000 description 1
- 241001133760 Acoelorraphe Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 241000123222 Hericium Species 0.000 description 1
- 241000251511 Holothuroidea Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 208000019790 abdominal distention Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000014106 fortified food Nutrition 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention relates to the field of edible fungus production, in particular to a water culture hericium erinaceus cultivation method. According to the method, the hericium erinaceus is subjected to water culture, and sodium selenite and MOF materials are added into the water culture nutrient solution, so that the hericium erinaceus are short in production period, fast in hypha growth and high in yield; MOF materials are added into the culture medium, so that the stability of selenium in the culture solution is improved, the utilization rate and conversion rate of selenium element are improved, and the selenium content in the finally produced hericium erinaceus entity is improved.
Description
Technical Field
The invention relates to the field of edible fungus production, in particular to a water culture hericium erinaceus cultivation method.
Background
Hericium erinaceus (academic name: hericium erinaceus (Bull.) Pers.) is fungus of genus Hericium of family odontology. The fruiting body is medium, large or large, has a diameter of 3.5-10 (30) cm, and meat quality, and has a head shape or inverted egg shape similar to that of monkey, so the fruiting body is named as Hericium erinaceus. The basal portion is narrow in the place where the basal portion is planted, and the basal portion of the manually cultivated hericium erinaceus is usually in a handle shape because of being longer than the bottle mouth or the plastic bag mouth. Except for the base, zhou Tiwai is covered with a bacterial spike. The length of the fungus thorn is 1-5 cm, the needle shape is thick 1-2 mm. The spores are on the surface of the fungus thorns, spherical, with the diameter of (5.5-7.5) micrometers (5-6) micrometers, oil drops are contained, and the spores are piled white. Hericium erinaceus are widely distributed in nature, mainly in broadleaf forests or needle-leaved and broadleaf mixed forests in the North temperate zone, such as western European, north American, japanese, russian and the like. In China, the water treatment agent is mainly distributed in the northeast great and small Khingan, the northwest Tianshan, the Altaishan, the west Himalayan mountain and the forest areas of the southwest transverse mountain, including the provinces and autonomous areas of Heilongjiang, jilin, inner Mongolia, hebei, henan, shanxi, gansu, sichuan, hubei, hunan, guangxi, yunnan, tibet, zhejiang, fujian and the like. Hericium erinaceus is not only an edible precious product in China, but also an important medicinal fungus. Hericium erinaceus is one of the eight major "delicacies" in China, and has the ancient theory of "delicacies Hericium erinaceus and sea-taste nidus Collocaliae", and is parallel to bear's palm, sea cucumber and shark fin to form four famous dishes, and the obtained dish is tender in meat, delicious, rich in nutrition and superior in color, smell and taste. In addition, the hericium erinaceus is also a traditional and valuable Chinese medicinal material in China, and has the functions of nourishing and building body, helping digestion and benefiting five viscera. Modern researches have shown that it contains active ingredients such as polypeptide, polysaccharide, fat and protein, etc., and has certain curative effects on digestive tract tumor, gastric ulcer, duodenal ulcer, gastritis, abdominal distention, etc.
Selenium-enriched foods are increasingly attracting attention as good supplements for selenium, and for example, GB1903.22-2016 also specifies that the total selenium content in selenium-enriched edible fungus powder is 180-400 mg/kg. In the prior art, publication No. CN109337895B discloses a production method of high-quality high-yield selenium-enriched hericium erinaceus strains, but the method is complex in process and requires high-voltage electrostatic field treatment, and meanwhile, components in a fermentation medium are complex and difficult to obtain.
Disclosure of Invention
The invention aims to provide a water culture hericium erinaceus cultivation method which is used for solving the problem that the selenium content in edible fungi, particularly hericium erinaceus, is not high.
The invention is realized by the following technical scheme:
a water culture Hericium erinaceus cultivation method mainly comprises the following steps:
step 1, preparing a hericium erinaceus strain culture solution;
step 2, inoculating a hericium erinaceus strain into the culture solution, performing shading and airtight culture at the culture temperature of 24 ℃ for 7 days to obtain a hericium erinaceus liquid strain;
step 3, filling the hericium erinaceus water culture nutrient solution into a water culture container, and inoculating a hericium erinaceus liquid strain into the water culture container in a sterile environment after sterilization, wherein the mass of the hericium erinaceus liquid strain is 10% -20% of that of the hericium erinaceus water culture nutrient solution; placing the inoculated water culture container into a culture chamber at 25-27.5 ℃ for fermentation culture for 7-15 days; shaking for 1-2 times daily in the first 7 days, and standing for culturing after 7 days until white particles appear; after white particles appear, harvesting after 7-10 days of illumination in daytime;
the formula of the hericium erinaceus water culture nutrient solution comprises glucose, peptone, potassium dihydrogen phosphate, urea, EDTA, manganese sulfate, copper sulfate, zinc sulfate, magnesium sulfate, calcium nitrate, sodium selenite UiO-66 and water.
Further, the preparation of the hericium erinaceus strain culture solution comprises the following steps: adding vitamin B1, glucose, magnesium sulfate, potassium dihydrogen phosphate and peptone into 500mL water, heating to 40deg.C until the components are completely dissolved, cooling to room temperature, adjusting pH to 7.4, metering volume to 1000mL with water, and sterilizing the culture solution; the mass percentages of the components in each 1000mL strain culture solution are 0.8 percent of peptone, 2 percent of glucose, 0.1 percent of magnesium sulfate, 0.1 percent of monopotassium phosphate and 0.002 percent of vitamin B.
Further, each 1000mL of nutrient solution contains 20g of glucose, 8g of peptone, 0.7g of potassium dihydrogen phosphate, 0.05g of urea, 0.0122 g of EDTAs, 0.016g of manganese sulfate, 0.002g of copper sulfate, 0.002g of zinc sulfate, 0.38g of magnesium sulfate, 1.0g of calcium nitrate, 0.2g of sodium selenite and 0.2g of UiO-66, and the solvent is water.
Further, the synthetic method of the UiO-66 comprises the following steps: 2.3g ZrCl was taken 4 Adding the mixture into 300mL of DMF, adding the mixture into 10 mL fuming hydrochloric acid, stirring, and adding 10mmol of terephthalic acid; 0.25mL of H was added 2 O and 50mL DMF, the mixed solution was heated at 95℃for 100h, cooled to room temperature, and the mixed solution was filtered and dried to give UiO-66.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the invention utilizes the liquid culture technology to produce the hericium erinaceus, and has short production period, quick hypha growth and high yield; MOF (UiO-66) materials are added into the culture medium, so that the stability of selenium in the culture solution is improved, the utilization rate and conversion rate of selenium element are improved, and the selenium content in the finally produced hericium erinaceus entity is improved; meanwhile, the culture process is simple, and complex equipment is not needed.
Detailed Description
The present invention will be described in further detail with reference to the following examples, for the purpose of making the objects, technical solutions and advantages of the present invention more apparent, and the description thereof is merely illustrative of the present invention and not intended to be limiting.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
Example 1
A water culture Hericium erinaceus cultivation method mainly comprises the following steps:
step 1, preparing a hericium erinaceus strain culture solution: adding 500mL of water into vitamin B1, glucose, magnesium sulfate, potassium dihydrogen phosphate and peptone, heating to 40 ℃ until the components are completely dissolved, cooling to room temperature, adjusting the pH to 7.4, fixing the volume to 1000mL with water, and sterilizing the culture solution; the mass percentages of the components in each 1000mL of strain culture solution are 0.8 percent of peptone, 2 percent of glucose, 0.1 percent of magnesium sulfate, 0.1 percent of potassium dihydrogen phosphate and 0.002 percent of vitamin B;
step 2, inoculating a hericium erinaceus strain into the culture solution, performing shading and airtight culture at the culture temperature of 24 ℃ for 7 days to obtain a hericium erinaceus liquid strain;
step 3, filling the hericium erinaceus water culture nutrient solution into a water culture container, and inoculating a hericium erinaceus liquid strain into the water culture container in a sterile environment after sterilization, wherein the mass of the hericium erinaceus liquid strain is 10% -20% of that of the hericium erinaceus water culture nutrient solution; placing the inoculated water culture container into a culture chamber at 25-27.5 ℃ for fermentation culture for 7-15 days; shaking for 1-2 times daily in the first 7 days, and standing for culturing after 7 days until white particles appear; after white particles appear, harvesting after 7-10 days of illumination in daytime.
The formula of the hericium erinaceus water culture nutrient solution comprises the following components:
each 1000mL nutrient solution contains 20g of glucose, 8g of peptone, 0.7g of monopotassium phosphate, 0.05g of urea, 0.012g of EDTA0.016 g of manganese sulfate, 0.002g of copper sulfate, 0.002g of zinc sulfate, 0.38g of magnesium sulfate, 1.0g of calcium nitrate, 0.2g of sodium selenite and 0.2g of UiO-66, and the solvent is water.
The synthetic method of the UiO-66 comprises the following steps: 2.3g (10 mmol ZrCl) was taken 4 ) To 300mL of DMF was added, followed by 10 mL fuming hydrochloric acid, stirred, and 10mmol of terephthalic acid was added. 0.25mL of H was added 2 O and 50mL DMF, the mixed solution was heated at 95℃for 100h, cooled to room temperature, and the mixed solution was filtered and dried to give UiO-66.
The collected Hericium erinaceus is measured according to GB 5009.93-2017, and the selenium content in the Hericium erinaceus is 93mg/kg.
Comparative example 1
The formula of the hericium erinaceus water culture nutrient solution is different from that of the hericium erinaceus water culture nutrient solution in the comparative example compared with the hericium erinaceus water culture nutrient solution in the example 1, and the hericium erinaceus water culture nutrient solution is specifically as follows.
A water culture Hericium erinaceus cultivation method mainly comprises the following steps:
step 1, preparing a hericium erinaceus strain culture solution: adding 500mL of water into vitamin B1, glucose, magnesium sulfate, potassium dihydrogen phosphate and peptone, heating to 40 ℃ until the components are completely dissolved, cooling to room temperature, adjusting the pH to 7.4, fixing the volume to 1000mL with water, and sterilizing the culture solution; the mass percentages of the components in each 1000mL of strain culture solution are 0.8 percent of peptone, 2 percent of glucose, 0.1 percent of magnesium sulfate, 0.1 percent of potassium dihydrogen phosphate and 0.002 percent of vitamin B;
step 2, inoculating a hericium erinaceus strain into the culture solution, performing shading and airtight culture at the culture temperature of 24 ℃ for 7 days to obtain a hericium erinaceus liquid strain;
step 3, filling the hericium erinaceus water culture nutrient solution into a water culture container, and inoculating a hericium erinaceus liquid strain into the water culture container in a sterile environment after sterilization, wherein the mass of the hericium erinaceus liquid strain is 10% -20% of that of the hericium erinaceus water culture nutrient solution; placing the inoculated water culture container into a culture chamber at 25-27.5 ℃ for fermentation culture for 7-15 days; shaking for 1-2 times daily in the first 7 days, and standing for culturing after 7 days until white particles appear; after white particles appear, harvesting after 7-10 days of illumination in daytime.
The formula of the hericium erinaceus water culture nutrient solution comprises the following components:
each 1000mL of nutrient solution contains 20g of glucose, 8g of peptone, 0.7g of monopotassium phosphate, 0.05g of urea, 0.012g of EDTA0.016 g of manganese sulfate, 0.002g of copper sulfate, 0.002g of zinc sulfate, 0.38g of magnesium sulfate, 1.0g of calcium nitrate and 0.2g of sodium selenite, and the solvent is water.
The collected Hericium erinaceus is measured according to GB 5009.93-2017, and the selenium content in the Hericium erinaceus is 23mg/kg.
Comparative example 2
The formula of the hericium erinaceus water culture nutrient solution is different from that of the hericium erinaceus water culture nutrient solution in the comparative example compared with the hericium erinaceus water culture nutrient solution in the example 1, and the hericium erinaceus water culture nutrient solution is specifically as follows.
A water culture Hericium erinaceus cultivation method mainly comprises the following steps:
step 1, preparing a hericium erinaceus strain culture solution: adding 500mL of water into vitamin B1, glucose, magnesium sulfate, potassium dihydrogen phosphate and peptone, heating to 40 ℃ until the components are completely dissolved, cooling to room temperature, adjusting the pH to 7.4, fixing the volume to 1000mL with water, and sterilizing the culture solution; the mass percentages of the components in each 1000mL of strain culture solution are 0.8 percent of peptone, 2 percent of glucose, 0.1 percent of magnesium sulfate, 0.1 percent of potassium dihydrogen phosphate and 0.002 percent of vitamin B;
step 2, inoculating a hericium erinaceus strain into the culture solution, performing shading and airtight culture at the culture temperature of 24 ℃ for 7 days to obtain a hericium erinaceus liquid strain;
step 3, filling the hericium erinaceus water culture nutrient solution into a water culture container, and inoculating a hericium erinaceus liquid strain into the water culture container in a sterile environment after sterilization, wherein the mass of the hericium erinaceus liquid strain is 10% -20% of that of the hericium erinaceus water culture nutrient solution; placing the inoculated water culture container into a culture chamber at 25-27.5 ℃ for fermentation culture for 7-15 days; shaking for 1-2 times daily in the first 7 days, and standing for culturing after 7 days until white particles appear; after white particles appear, harvesting after 7-10 days of illumination in daytime.
The formula of the hericium erinaceus water culture nutrient solution comprises the following components:
each 1000mL nutrient solution contains 20g of glucose, 8g of peptone, 0.7g of monopotassium phosphate, 0.05g of urea, 0.012g of EDTA0.016 g of manganese sulfate, 0.002g of copper sulfate, 0.002g of zinc sulfate, 0.38g of magnesium sulfate, 1.0g of calcium nitrate, 0.2g of sodium selenite and 0.2g of UiO-67, and the solvent is water.
The synthetic method of the UiO-67 comprises the following steps: 2.3g (10 mmol ZrCl) was taken 4 ) To 300mL of DMF was added, followed by 10 mL fuming hydrochloric acid, stirred, and 10mmol of 4,4' -biphthalic acid was added. 0.25mL of H was added 2 O and 50mL DMF, the mixed solution was heated at 95℃for 100h, cooled to room temperature, and the mixed solution was filtered and dried to give UiO-67.
The collected Hericium erinaceus is measured according to GB 5009.93-2017, and the selenium content in the Hericium erinaceus is 33mg/kg.
Comparative example 3
The formula of the hericium erinaceus water culture nutrient solution is different from that of the hericium erinaceus water culture nutrient solution in the comparative example compared with the hericium erinaceus water culture nutrient solution in the example 1, and the hericium erinaceus water culture nutrient solution is specifically as follows.
A water culture Hericium erinaceus cultivation method mainly comprises the following steps:
step 1, preparing a hericium erinaceus strain culture solution: adding 500mL of water into vitamin B1, glucose, magnesium sulfate, potassium dihydrogen phosphate and peptone, heating to 40 ℃ until the components are completely dissolved, cooling to room temperature, adjusting the pH to 7.4, fixing the volume to 1000mL with water, and sterilizing the culture solution; the mass percentages of the components in each 1000mL of strain culture solution are 0.8 percent of peptone, 2 percent of glucose, 0.1 percent of magnesium sulfate, 0.1 percent of potassium dihydrogen phosphate and 0.002 percent of vitamin B;
step 2, inoculating a hericium erinaceus strain into the culture solution, performing shading and airtight culture at the culture temperature of 24 ℃ for 7 days to obtain a hericium erinaceus liquid strain;
step 3, filling the hericium erinaceus water culture nutrient solution into a water culture container, and inoculating a hericium erinaceus liquid strain into the water culture container in a sterile environment after sterilization, wherein the mass of the hericium erinaceus liquid strain is 10% -20% of that of the hericium erinaceus water culture nutrient solution; placing the inoculated water culture container into a culture chamber at 25-27.5 ℃ for fermentation culture for 7-15 days; shaking for 1-2 times daily in the first 7 days, and standing for culturing after 7 days until white particles appear; after white particles appear, harvesting after 7-10 days of illumination in daytime.
The formula of the hericium erinaceus water culture nutrient solution comprises the following components:
each 1000mL nutrient solution contains 20g of glucose, 8g of peptone, 0.7g of monopotassium phosphate, 0.05g of urea, 0.012g of EDTA0.016 g of manganese sulfate, 0.002g of copper sulfate, 0.002g of zinc sulfate, 0.38g of magnesium sulfate, 1.0g of calcium nitrate, 0.2g of sodium selenite and 0.2g of UiO-68, and the solvent is water.
The synthetic method of the UiO-68 comprises the following steps: 2.3g (10 mmol ZrCl) was taken 4 ) To 300mL of DMF was added, followed by 10 mL fuming hydrochloric acid, and 10mmol of 1, 4-bis (4-carboxyphenyl) benzene was added with stirring. Then, 0.25mL H2O and 50mL DMF were added, the mixture was heated at 95℃for 100h, cooled to room temperature, and the mixture was filtered and dried to give UiO-68.
The collected Hericium erinaceus is measured according to GB 5009.93-2017, and the selenium content in the Hericium erinaceus is 21mg/kg.
From the data in example 1 and comparative examples 2-3, it can be seen that the addition of MOF material to a sodium selenite-containing nutrient solution does not necessarily result in an increase in selenium content in Hericium erinaceus, wherein the increase in selenium content is significant when UiO-66 is added, and that UiO-67 and UiO-68 are not particularly significant increases as compared to UiO-66. The ligand in UiO-66 is presumed to be caused by terephthalic acid, and the ligand in UiO-66 has shorter length than UiO-67 and UiO-68, which is more favorable for absorption of sodium selenite by Hericium erinaceus in nutrient solution. The patent publication No. CN109337895B in the prior art discloses a production method of high-quality high-yield selenium-enriched hericium erinaceus strains, wherein the total selenium content of the hericium erinaceus is 90.38mg/kg, and the selenium content of the hericium erinaceus is 93mg/kg in the embodiment using UiO-66 in the invention, and compared with the prior art, the selenium content of the hericium erinaceus is higher.
Claims (2)
1. A water culture hericium erinaceus cultivation method, which comprises the following steps: step 1, preparing a hericium erinaceus strain culture solution; step 2, inoculating a hericium erinaceus strain into the culture solution, performing shading and airtight culture at the culture temperature of 24 ℃ for 7 days to obtain a hericium erinaceus liquid strain; step 3, filling the hericium erinaceus water culture nutrient solution into a water culture container, and inoculating a hericium erinaceus liquid strain into the water culture container in a sterile environment after sterilization, wherein the mass of the hericium erinaceus liquid strain is 10% -20% of that of the hericium erinaceus water culture nutrient solution; placing the inoculated water culture container into a culture chamber at 25-27.5 ℃ for fermentation culture for 7-15 days; shaking for 1-2 times daily in the first 7 days, and standing for culturing after 7 days until white particles appear; after white particles appear, harvesting after 7-10 days of illumination in daytime; wherein the formula of the hericium erinaceus water culture nutrient solution comprises glucose, peptone, potassium dihydrogen phosphate, urea, EDTA, manganese sulfate, copper sulfate, zinc sulfate, magnesium sulfate, calcium nitrate, sodium selenite, uiO-66 and water;
each 1000mL of the nutrient solution contains 20g of glucose, 8g of peptone, 0.7g of potassium dihydrogen phosphate, 0.05g of urea, 0.0120 g of EDTAs, 0.016g of manganese sulfate, 0.002g of copper sulfate, 0.002g of zinc sulfate, 0.38g of magnesium sulfate, 1.0g of calcium nitrate, 0.2g of sodium selenite and 0.2g of UiO-66, and the solvent is water;
the synthetic method of the UiO-66 comprises the following steps: 2.3g ZrCl4 was taken and added to 300mL DMF, then to 10 mL fuming hydrochloric acid, stirred, and 10mmol terephthalic acid was added; then, 0.25mL H2O and 50mL DMF were added, the mixture was heated at 95℃for 100h, cooled to room temperature, and the mixture was filtered and dried to give UiO-66.
2. The method for cultivating water-cultured hericium erinaceus according to claim 1, wherein preparing the culture solution of hericium erinaceus strains comprises the following steps: adding vitamin B1, glucose, magnesium sulfate, potassium dihydrogen phosphate and peptone into 500mL water, heating to 40deg.C until the components are completely dissolved, cooling to room temperature, adjusting pH to 7.4, metering volume to 1000mL with water, and sterilizing the culture solution; the mass percentages of the components in each 1000mL strain culture solution are 0.8 percent of peptone, 2 percent of glucose, 0.1 percent of magnesium sulfate, 0.1 percent of monopotassium phosphate and 0.002 percent of vitamin B.
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