CN103430776A - Silkworm pupa-worm grass culture method capable of improving yield and quality - Google Patents
Silkworm pupa-worm grass culture method capable of improving yield and quality Download PDFInfo
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Abstract
The invention discloses a silkworm pupa-worm grass culture method capable of improving yield and quality. The method is characterized by comprising the steps including preparation of liquid spawn, pupa sterilization, inoculation, cultivation in dark, wound treatment of pupas, cultivation in light, harvest and the like. Compared with the conventional culture method, the silkworm pupa-worm grass culture method disclosed by the invention is short in grass germination time, great in fruit body number, vigorous in growth and short in growth period, so that not only can the yield of the worm grass be increased, but also the growth period can be greatly shortened, and thus the economic benefits are greatly increased.
Description
Technical field
The present invention relates to a kind of method that improves silkworm chrysalis Cordyceps sinensis output and quality.
Background technology
The cultural method of traditional silkworm chrysalis Cordyceps sinensis, incubation time is long, generally needs 60-80 days, and stroma quantity is few, is generally the 3-5 bar, and its output and quality all remain further to be improved.
Summary of the invention
The object of the present invention is to provide a kind of method is easy, incubation time is short, output is high raising output and the silkworm chrysalis Cordyceps sinensis cultural method of quality.
Technical solution of the present invention is:
A kind of silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, is characterized in that: comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 10 ~ 30 parts of glucose, potassium dihydrogen phosphate 1-5 part, magnesium sulfate 1-5 part, 5 ~ 10 parts of peptones, yeast soaks powder 2-5 part, vitaminB10 .1 ~ 0.5 part, 1000 parts, water;
The bacterial classification preparation: the liquid nutrient medium prepared is sub-packed in conical flask, 200 ~ 250 milliliters every bottle, sealing, under 120 ℃ ~ 130 ℃ conditions, sterilizing is 18 ~ 30 minutes; Take out medium after sterilizing, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; Under 15 ~ 30 ℃, in shaking table, 5-10 days is cultivated in concussion;
(2) pupal cell sterilization:
Get fresh and alive tussah chrysalis, with alcohol-pickled 1-3 minute, take out and be put into ultraviolet sterilization 15-30 minute on superclean bench;
(3) inoculation:
Liquid spawn is injected in pupal cell to each pupal cell 0.1-0.5 milliliter bacterial classification with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, each bottle 3-5, be placed in constant incubator, cultivate 5-10 days for 18-25 ℃, after it is stiff, take out; Under natural daylight, 18-25 ℃, cultivate 5-10 days under air humidity 50%-65% condition;
(5) the pupal cell wound is processed:
Cut long 2-3 centimetre at the pupal cell outside of belly with scissors, the openning 2-4 bar of dark 2-4 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, temperature 18-25 ℃, continue to cultivate 25-30 days under air humidity 65%-85% condition;
(7) gather
When light is cultured to fruit body and grows to the 5-8 centimeter length, gathered.
When in step (1) prepared by bacterial classification, shaking speed is 120 rev/mins.
Step is pupal cell when sterilization (2), and the mass concentration of alcohol is 75%.
The present invention is when silkworm chrysalis ossifys latter 5-10 days, silkworm chrysalis is carried out to the wound processing, make the mycelia be enclosed in the silkworm chrysalis body touch air and light, processing not only can be shortened going out the grass time of silkworm chrysalis Cordyceps sinensis greatly like this, also can increase grass output amount simultaneously, make fruit body look more slightly more strong, maturation time is shortened greatly.Shorten to 40-50 days from being inoculated into the stroma maturation by normal 60-80 days, more than stroma quantity is increased to the 15-25 bar by the 3-5 bar, length can reach 7-10 centimetre, fruit-body color after oven dry is bright-coloured, neatly, quality is high, and output improves more than 25% than common cultural method.With traditional cultural method relatively this cultural method to have the grass time short, fruit body quantity is many, grows vigorous, growth cycle shortens, and not only can improve the output of Chinese caterpillar fungus, has also greatly shortened growth cycle, and economic benefit is improved significantly simultaneously.
Below in conjunction with embodiment, the invention will be further described.
Embodiment
Embodiment 1:
A kind of silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: glucose 20 grams, potassium dihydrogen phosphate 3 grams, magnesium sulfate 4 grams, peptone 10 grams, vitamin B1 0.3 gram, yeast soaks powder 2 grams, water 1000ml;
The bacterial classification preparation: the liquid nutrient medium prepared is sub-packed in conical flask, 220 milliliters every bottle, sealing, under 130 ℃ of conditions, sterilizing is 18 minutes; Take out medium after sterilizing, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 20 ℃, in the shaking table of 120 rev/mins, concussion is cultivated 7 days;
(2) pupal cell sterilization:
Get tussah chrysalis, with 75% alcohol-pickled 1 minute, take out and be put on superclean bench ultraviolet sterilization 22 minutes;
(3) inoculation:
Liquid spawn is injected in pupal cell to 0.5 milliliter of bacterial classification of each pupal cell with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, and 3, each bottle, be placed in constant incubator, cultivates 10 days for 20 ℃, after it is stiff, takes out; Under natural daylight, 22 ℃, under air humidity 60% condition, cultivate 8 days;
(5) the pupal cell wound is processed:
Cut long 2 centimetres at the pupal cell outside of belly with scissors, 4, the openning of dark 3 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, 22 ℃ of temperature, continue under air humidity 75% condition to cultivate 28 days;
(7) gather
When light is cultured to fruit body and grows to the 5-8 centimeter length, gathered.
Embodiment 2:
A kind of silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: glucose 25 grams, potassium dihydrogen phosphate 5 grams, magnesium sulfate 1 gram, peptone 5 grams, vitamin B1 0.5 gram, yeast soaks powder 5 grams, water 1000ml;
The bacterial classification preparation: the liquid nutrient medium prepared is sub-packed in conical flask, 200 milliliters every bottle, sealing, under 125 ℃ of conditions, sterilizing is 25 minutes; Take out medium after sterilizing, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 25 ℃, in the shaking table of 120 rev/mins, concussion is cultivated 5 days;
(2) pupal cell sterilization:
Get tussah chrysalis, with 75% alcohol-pickled 2 minutes, take out and be put on superclean bench ultraviolet sterilization 30 minutes;
(3) inoculation:
Liquid spawn is injected in pupal cell to 0.3 milliliter of bacterial classification of each pupal cell with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, and 4, each bottle, be placed in constant incubator, cultivates 8 days for 25 ℃, after it is stiff, takes out; Under natural daylight, 25 ℃, under air humidity 65% condition, cultivate 10 days;
(5) the pupal cell wound is processed:
Cut long 3 centimetres at the pupal cell outside of belly with scissors, 2, the openning of dark 4 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, 20 ℃ of temperature, continue under air humidity 67% condition to cultivate 30 days;
(7) gather
When light is cultured to fruit body and grows to the 5-8 centimeter length, gathered.
Embodiment 3:
A kind of silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: glucose 30 grams, potassium dihydrogen phosphate 1 gram, magnesium sulfate 3 grams, peptone 6 grams, vitaminB10 .1 gram, yeast soaks powder 3 grams, water 1000ml;
The bacterial classification preparation: the liquid nutrient medium prepared is sub-packed in conical flask, 230 milliliters every bottle, sealing, under 120 ℃ of conditions, sterilizing is 26 minutes; Take out medium after sterilizing, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 26 ℃, in the shaking table of 120 rev/mins, concussion is cultivated 5 days;
(2) pupal cell sterilization:
Get tussah chrysalis, with 75% alcohol-pickled 2 minutes, take out and be put on superclean bench ultraviolet sterilization 30 minutes;
(3) inoculation:
Liquid spawn is injected in pupal cell to 0.3 milliliter of bacterial classification of each pupal cell with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, and 5, each bottle, be placed in constant incubator, cultivates 7 days for 18 ℃, after it is stiff, takes out; Under natural daylight, 25 ℃, under air humidity 65% condition, cultivate 9 days;
(5) the pupal cell wound is processed:
Cut long 2.5 centimetres at the pupal cell outside of belly with scissors, 3, the openning of dark 3 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, 23 ℃ of temperature, continue under air humidity 68% condition to cultivate 30 days;
(7) gather
When light is cultured to fruit body and grows to the 5-8 centimeter length, gathered.
Claims (3)
1. a silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, is characterized in that: comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 10 ~ 30 parts of glucose, 1 ~ 5 part of potassium dihydrogen phosphate, 1 ~ 5 part, magnesium sulfate, 5 ~ 10 parts of peptones, yeast soaks powder 2-5 part, vitaminB10 .1 ~ 0.5 part, 1000 parts, water;
The bacterial classification preparation: the liquid nutrient medium prepared is sub-packed in conical flask, 200 ~ 250 milliliters every bottle, sealing, under 120 ℃ ~ 130 ℃ conditions, sterilizing is 18 ~ 30 minutes; Take out medium after sterilizing, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; Under 15 ~ 30 ℃, in shaking table, 5-10 days is cultivated in concussion;
(2) pupal cell sterilization:
Get fresh and alive tussah chrysalis, with alcohol-pickled 1-3 minute, take out and be put into ultraviolet sterilization 15-30 minute on superclean bench;
(3) inoculation:
Liquid spawn is injected in pupal cell to each pupal cell 0.1-0.5 milliliter bacterial classification with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, each bottle 3-5, sealing, be placed in constant incubator, cultivates 5-10 days under 18-25 ℃, after it is stiff, takes out; Under natural daylight, 18-25 ℃, cultivate 5-10 days under air humidity 50%-65% condition;
(5) the pupal cell wound is processed:
Cut long 2-3 centimetre at the pupal cell outside of belly with scissors, the openning 2-4 bar of dark 2-4 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, temperature 18-25 ℃, continue to cultivate 25-30 days under air humidity 65%-85% condition;
(7) gather
When light is cultured to fruit body and grows to the 5-8 centimeter length, gathered.
2. the silkworm chrysalis Cordyceps sinensis cultural method of raising output according to claim 1 and quality is characterized in that: when in step (1) prepared by bacterial classification, shaking speed is 120 rev/mins.
3. the silkworm chrysalis Cordyceps sinensis cultural method of raising output according to claim 1 and quality is characterized in that: step is (2) during the pupal cell sterilization, and the mass concentration of alcohol is 75%.
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| CN103688759A (en) * | 2013-12-20 | 2014-04-02 | 凌中鑫 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
| CN103704023A (en) * | 2013-12-24 | 2014-04-09 | 沈阳师范大学 | Method for cultivating functional cordyceps militaris |
| CN103749149A (en) * | 2013-12-20 | 2014-04-30 | 凌中鑫 | Method for cultivating artificial worm grass with mealworm pupa as carrier |
| CN104137731A (en) * | 2013-12-23 | 2014-11-12 | 北京首诚航天农业生物科技有限公司 | Physical methods for controlling primordium differentiation quantity of cordyceps militaris |
| CN104303835A (en) * | 2014-09-29 | 2015-01-28 | 苏州家和农业生物科技研究中心 | Cultivation method of cordyceps sinensis |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN104904493A (en) * | 2015-04-08 | 2015-09-16 | 丹东中天柞蚕生物科技有限公司 | Method for artificially cultivating Chinese-oak-silkworm-pupa cordyceps militaris through yellow Chinese oak silkworm pupae |
| CN105660188A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Cultivation method for cordyceps militaris |
| CN105706738A (en) * | 2016-02-23 | 2016-06-29 | 张家港顺泰元生物科技有限公司 | Cordycepsmilitaris cultivation method |
| CN105724054A (en) * | 2016-02-23 | 2016-07-06 | 张家港顺泰元生物科技有限公司 | Liquid culture solution for dried cordyceps militaris and culture method for dried cordyceps militaris |
| CN105981583A (en) * | 2015-02-10 | 2016-10-05 | 保山学院 | Method for promoting growth of pendulous cordyceps militaris by adding fresh insect protein |
| CN106171526A (en) * | 2016-08-01 | 2016-12-07 | 鲁东大学 | The method cultivating growth Cordyceps militaris (L.) Link. in golden cicada |
| CN108633616A (en) * | 2018-05-11 | 2018-10-12 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving activity substance content in Cordyceps militaris |
| CN110373373A (en) * | 2018-04-13 | 2019-10-25 | 航天(北京)食品技术研究院 | A kind of cultural method of high-content Pentostatin cordyceps militaris link bacterial strain |
| CN110692434A (en) * | 2019-10-15 | 2020-01-17 | 吉林省蚕业科学研究院 | Method for culturing cordyceps sinensis of dichotomous rhinoceros |
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| CN112190602A (en) * | 2020-10-27 | 2021-01-08 | 苏州家和蚕业生物科技有限公司 | Preparation method of silkworm chrysalis cordyceps sinensis tablets |
| CN112205615A (en) * | 2020-10-27 | 2021-01-12 | 苏州家和蚕业生物科技有限公司 | Preparation method of silkworm chrysalis cordyceps sinensis concentrated solution |
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