CN105706738A

CN105706738A - Cordycepsmilitaris cultivation method

Info

Publication number: CN105706738A
Application number: CN201610097334.4A
Authority: CN
Inventors: 吴建军; 史余娟
Original assignee: Zhangjiagang Shuntaiyuan Biotechnology Co Ltd
Current assignee: Zhangjiagang Shuntaiyuan Biotechnology Co Ltd
Priority date: 2016-02-23
Filing date: 2016-02-23
Publication date: 2016-06-29

Abstract

The invention discloses a cordycepsmilitaris cultivation method.The method includes the steps of injecting strains in the body surfaces of the abdomens of live silkworms 5 years old, ensuring that no bacterium liquid flows out, sterilizing the bodies of the silkworms after inoculation is completed, putting the bodies of the silkworms in a constant-temperature sterile dark room with the temperature of 15-20 DEG C and the humidity of 50-70% for spawn running, removing necrotic bodies after the bodies of the silkworms are inactivated, sterilizing the complete bodies of the silkworms again, putting the bodies in a bottle to be sealed, conducting cultivation at the humidity of 75% to 80% and the illumination of 200-250 lx, conducting harvesting 35-45 days after light sensing and grass germinating, and conducting drying to obtain cordycepsmilitaris.According to the method, by conducting inoculation on the body surfaces of the abdomens of the bodies of the silkworms, the necrosis rate and grass germination rate of the bodies of the silkworms both reach 99% or above, and the input cost of live silkworms is greatly reduced; meanwhile, chemical components and nutrient components of the cultivated cordycepsmilitaris are close to those of wild cordycepsmilitaris, and the nutrition value is high.

Description

A kind of breeding method of silkworm grass

Technical field

The present invention relates to a kind of Cordyceps Cultivating techniques field, particularly to the breeding method of a kind of silkworm grass.

Background technology

Wild cordyceps is rare Chinese medicine, and its natural resources is rare, and price value is expensive, it is difficult to universal, and its distributional region is narrow, and natural parasitic rate is low, and resource is very limited.Artificial destruction is suffered in Cordyceps main product ground ecological environment in recent years, and resource reduces increasingly, causes yield to fall sharply, price sharp rises, and the market demand breach of Cordyceps constantly expands.And silkworm grass artificial culture and large-scale production can be good at alleviating above-mentioned contradiction.Silkworm grass artificial culture is the production, teaching & research agricultural high-tech project with relatively high-tech content and broad mass market prospect, is also consistent with the emerging strategic biological industry project of help from government policy.

And in the cultivating process of silkworm grass, because inoculating the problem of maneuver, the survival rate inoculating strain on live body silkworm body is relatively low, thus adding the production cost of silkworm grass.

Summary of the invention

It is an object of the invention to overcome the deficiencies in the prior art, it is provided that the breeding method of a kind of silkworm grass, adopt this breeding method, be effectively improved the rigid rate of silkworm grass, go out carelessness, reduce production cost.

For reaching above-mentioned purpose, the technical solution used in the present invention is: the breeding method of a kind of silkworm grass, comprises the steps: to be expelled to strain the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out；After inoculation, disinfection of silkworm body is placed in temperature be 15 ~ 20 DEG C, humidity be 50 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then loads in bottle and seal, humidity be 75 ~ 80%, illumination cultivates when being 2000 ~ 2500lx, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.

Preferably, the concentration of described strain is 200 ~ 300mg/L, and inoculum concentration is 1 ~ 2ml.

Preferably, the temperature sent out in bacterium process controls at 16 ~ 18 DEG C, and humid control is between 55 ~ 65%.

Preferably, the disinfectant solution adopted during disinfection of silkworm body is Methylpartricin Sodium Laurylsulfate, and its concentration is 0.5 ~ 0.7g/L.

Utilization due to technique scheme, the present invention compared with prior art has the advantage that the breeding method of the silkworm grass of the present invention, in seeded process, strain is seeded on the abdomen body-surface of live body 5 silkworm in age, thus the rigid rate of Bombyxmori Linnaeus can be made, and goes out carelessness and reaches more than 99%, and cultivate in an aseptic environment, largely avoided the pollution of miscellaneous bacteria, greatly reduce the input cost of live body Bombyxmori Linnaeus simultaneously.

Detailed description of the invention

Below in conjunction with specific embodiment, technical scheme is further elaborated.

Embodiment 1

The breeding method of a kind of silkworm grass, comprises the steps: to be inoculated into strain by the mode injected the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out；After inoculation, by disinfection of silkworm body, be placed in temperature be 16 ~ 18 DEG C, humidity be 50 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then load in bottle and seal, humidity be 75 ~ 80%, illumination cultivate when being 2000 ~ 2500lx, period, light application time is every 2 ~ 3 days, irradiate 48 ~ 72 hours, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.

Wherein, the strain adopted derives from wild Cordyceps, and during inoculation, the concentration of strain is 200mg/L, and inoculum concentration is 1ml.During disinfection of silkworm body, the disinfectant solution adopted is concentration is the Methylpartricin Sodium Laurylsulfate of 0.5g/L.

After tested, in embodiment 1, the rigid rate of silkworm body is 100%, to go out carelessness be 100%, without miscellaneous bacteria, containing cordycepin 8.06mg/g in prepared silkworm grass, and Cordyceps adenosine 1.80mg/g, polypeptide 488mg/g.

Embodiment 2

The breeding method of a kind of silkworm grass, comprises the steps: to be inoculated into strain by the mode injected the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out；After inoculation, by disinfection of silkworm body, be placed in temperature be 15 ~ 20 DEG C, humidity be 55 ~ 65% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then load in bottle and seal, humidity be 75 ~ 80%, illumination cultivate when being 2000 ~ 2500lx, period, light application time is every 3 days, irradiate 72 hours, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.

Wherein, the strain adopted derives from wild Cordyceps, and during inoculation, the concentration of strain is 250mg/L, and inoculum concentration is 1.5ml.During disinfection of silkworm body, the disinfectant solution adopted is concentration is the Methylpartricin Sodium Laurylsulfate of 0.7g/L.

After tested, in embodiment 2, the rigid rate of silkworm body is 100%, to go out carelessness be 99.9%, without miscellaneous bacteria, containing cordycepin 8.25mg/g in prepared silkworm grass, and Cordyceps adenosine 1.95mg/g, polypeptide 510mg/g.

Embodiment 3

The breeding method of a kind of silkworm grass, comprises the steps: to be inoculated into strain by the mode injected the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out；After inoculation, by disinfection of silkworm body, be placed in temperature be 16 ~ 20 DEG C, humidity be 55 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then load in bottle and seal, humidity be 75 ~ 80%, illumination cultivate when being 2300 ~ 2500lx, period, light application time is every 2 ~ 3 days, irradiate 48 ~ 72 hours, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.

Wherein, the strain adopted derives from wild Cordyceps, and during inoculation, the concentration of strain is 220mg/L, and inoculum concentration is 2ml.During disinfection of silkworm body, the disinfectant solution adopted is concentration is the Methylpartricin Sodium Laurylsulfate of 0.5g/L.

After tested, in embodiment 3, the rigid rate of silkworm body is 100%, to go out carelessness be 100%, without miscellaneous bacteria, containing cordycepin 8.12mg/g in prepared silkworm grass, and Cordyceps adenosine 2.01mg/g, polypeptide 508mg/g.

Above-described embodiment is only for illustrating technology design and the feature of the present invention; its object is to allow person skilled in the art will appreciate that present disclosure and to be carried out; can not limit the scope of the invention with this; all equivalences made according to spirit of the invention change or modify, and all should be encompassed in protection scope of the present invention.

Claims

1. the breeding method of a silkworm grass, it is characterised in that comprise the steps: to be expelled to strain the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out；After inoculation, disinfection of silkworm body is placed in temperature be 15 ~ 20 DEG C, humidity be 50 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then loads in bottle and seal, humidity be 75 ~ 80%, illumination cultivates when being 2000 ~ 2500lx, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.

2. the breeding method of silkworm according to claim 1 grass, it is characterised in that the concentration of described strain is 200 ~ 300mg/L, and inoculum concentration is 1 ~ 2ml.

3. the breeding method of silkworm according to claim 1 grass, it is characterised in that the temperature sent out in bacterium process controls at 16 ~ 18 DEG C, and humid control is between 55 ~ 65%.

4. the breeding method according to the arbitrary described silkworm grass of claims 1 to 3, it is characterised in that the disinfectant solution adopted during disinfection of silkworm body is Methylpartricin Sodium Laurylsulfate, and its concentration is 0.5 ~ 0.7g/L.