CN105706738A - Cordycepsmilitaris cultivation method - Google Patents

Cordycepsmilitaris cultivation method Download PDF

Info

Publication number
CN105706738A
CN105706738A CN201610097334.4A CN201610097334A CN105706738A CN 105706738 A CN105706738 A CN 105706738A CN 201610097334 A CN201610097334 A CN 201610097334A CN 105706738 A CN105706738 A CN 105706738A
Authority
CN
China
Prior art keywords
silkworm
grass
silkworms
bodies
cordycepsmilitaris
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610097334.4A
Other languages
Chinese (zh)
Inventor
吴建军
史余娟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhangjiagang Shuntaiyuan Biotechnology Co Ltd
Original Assignee
Zhangjiagang Shuntaiyuan Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhangjiagang Shuntaiyuan Biotechnology Co Ltd filed Critical Zhangjiagang Shuntaiyuan Biotechnology Co Ltd
Priority to CN201610097334.4A priority Critical patent/CN105706738A/en
Publication of CN105706738A publication Critical patent/CN105706738A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The invention discloses a cordycepsmilitaris cultivation method.The method includes the steps of injecting strains in the body surfaces of the abdomens of live silkworms 5 years old, ensuring that no bacterium liquid flows out, sterilizing the bodies of the silkworms after inoculation is completed, putting the bodies of the silkworms in a constant-temperature sterile dark room with the temperature of 15-20 DEG C and the humidity of 50-70% for spawn running, removing necrotic bodies after the bodies of the silkworms are inactivated, sterilizing the complete bodies of the silkworms again, putting the bodies in a bottle to be sealed, conducting cultivation at the humidity of 75% to 80% and the illumination of 200-250 lx, conducting harvesting 35-45 days after light sensing and grass germinating, and conducting drying to obtain cordycepsmilitaris.According to the method, by conducting inoculation on the body surfaces of the abdomens of the bodies of the silkworms, the necrosis rate and grass germination rate of the bodies of the silkworms both reach 99% or above, and the input cost of live silkworms is greatly reduced; meanwhile, chemical components and nutrient components of the cultivated cordycepsmilitaris are close to those of wild cordycepsmilitaris, and the nutrition value is high.

Description

A kind of breeding method of silkworm grass
Technical field
The present invention relates to a kind of Cordyceps Cultivating techniques field, particularly to the breeding method of a kind of silkworm grass.
Background technology
Wild cordyceps is rare Chinese medicine, and its natural resources is rare, and price value is expensive, it is difficult to universal, and its distributional region is narrow, and natural parasitic rate is low, and resource is very limited.Artificial destruction is suffered in Cordyceps main product ground ecological environment in recent years, and resource reduces increasingly, causes yield to fall sharply, price sharp rises, and the market demand breach of Cordyceps constantly expands.And silkworm grass artificial culture and large-scale production can be good at alleviating above-mentioned contradiction.Silkworm grass artificial culture is the production, teaching & research agricultural high-tech project with relatively high-tech content and broad mass market prospect, is also consistent with the emerging strategic biological industry project of help from government policy.
And in the cultivating process of silkworm grass, because inoculating the problem of maneuver, the survival rate inoculating strain on live body silkworm body is relatively low, thus adding the production cost of silkworm grass.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that the breeding method of a kind of silkworm grass, adopt this breeding method, be effectively improved the rigid rate of silkworm grass, go out carelessness, reduce production cost.
For reaching above-mentioned purpose, the technical solution used in the present invention is: the breeding method of a kind of silkworm grass, comprises the steps: to be expelled to strain the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out;After inoculation, disinfection of silkworm body is placed in temperature be 15 ~ 20 DEG C, humidity be 50 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then loads in bottle and seal, humidity be 75 ~ 80%, illumination cultivates when being 2000 ~ 2500lx, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.
Preferably, the concentration of described strain is 200 ~ 300mg/L, and inoculum concentration is 1 ~ 2ml.
Preferably, the temperature sent out in bacterium process controls at 16 ~ 18 DEG C, and humid control is between 55 ~ 65%.
Preferably, the disinfectant solution adopted during disinfection of silkworm body is Methylpartricin Sodium Laurylsulfate, and its concentration is 0.5 ~ 0.7g/L.
Utilization due to technique scheme, the present invention compared with prior art has the advantage that the breeding method of the silkworm grass of the present invention, in seeded process, strain is seeded on the abdomen body-surface of live body 5 silkworm in age, thus the rigid rate of Bombyxmori Linnaeus can be made, and goes out carelessness and reaches more than 99%, and cultivate in an aseptic environment, largely avoided the pollution of miscellaneous bacteria, greatly reduce the input cost of live body Bombyxmori Linnaeus simultaneously.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme is further elaborated.
Embodiment 1
The breeding method of a kind of silkworm grass, comprises the steps: to be inoculated into strain by the mode injected the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out;After inoculation, by disinfection of silkworm body, be placed in temperature be 16 ~ 18 DEG C, humidity be 50 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then load in bottle and seal, humidity be 75 ~ 80%, illumination cultivate when being 2000 ~ 2500lx, period, light application time is every 2 ~ 3 days, irradiate 48 ~ 72 hours, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.
Wherein, the strain adopted derives from wild Cordyceps, and during inoculation, the concentration of strain is 200mg/L, and inoculum concentration is 1ml.During disinfection of silkworm body, the disinfectant solution adopted is concentration is the Methylpartricin Sodium Laurylsulfate of 0.5g/L.
After tested, in embodiment 1, the rigid rate of silkworm body is 100%, to go out carelessness be 100%, without miscellaneous bacteria, containing cordycepin 8.06mg/g in prepared silkworm grass, and Cordyceps adenosine 1.80mg/g, polypeptide 488mg/g.
Embodiment 2
The breeding method of a kind of silkworm grass, comprises the steps: to be inoculated into strain by the mode injected the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out;After inoculation, by disinfection of silkworm body, be placed in temperature be 15 ~ 20 DEG C, humidity be 55 ~ 65% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then load in bottle and seal, humidity be 75 ~ 80%, illumination cultivate when being 2000 ~ 2500lx, period, light application time is every 3 days, irradiate 72 hours, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.
Wherein, the strain adopted derives from wild Cordyceps, and during inoculation, the concentration of strain is 250mg/L, and inoculum concentration is 1.5ml.During disinfection of silkworm body, the disinfectant solution adopted is concentration is the Methylpartricin Sodium Laurylsulfate of 0.7g/L.
After tested, in embodiment 2, the rigid rate of silkworm body is 100%, to go out carelessness be 99.9%, without miscellaneous bacteria, containing cordycepin 8.25mg/g in prepared silkworm grass, and Cordyceps adenosine 1.95mg/g, polypeptide 510mg/g.
Embodiment 3
The breeding method of a kind of silkworm grass, comprises the steps: to be inoculated into strain by the mode injected the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out;After inoculation, by disinfection of silkworm body, be placed in temperature be 16 ~ 20 DEG C, humidity be 55 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then load in bottle and seal, humidity be 75 ~ 80%, illumination cultivate when being 2300 ~ 2500lx, period, light application time is every 2 ~ 3 days, irradiate 48 ~ 72 hours, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.
Wherein, the strain adopted derives from wild Cordyceps, and during inoculation, the concentration of strain is 220mg/L, and inoculum concentration is 2ml.During disinfection of silkworm body, the disinfectant solution adopted is concentration is the Methylpartricin Sodium Laurylsulfate of 0.5g/L.
After tested, in embodiment 3, the rigid rate of silkworm body is 100%, to go out carelessness be 100%, without miscellaneous bacteria, containing cordycepin 8.12mg/g in prepared silkworm grass, and Cordyceps adenosine 2.01mg/g, polypeptide 508mg/g.
Above-described embodiment is only for illustrating technology design and the feature of the present invention; its object is to allow person skilled in the art will appreciate that present disclosure and to be carried out; can not limit the scope of the invention with this; all equivalences made according to spirit of the invention change or modify, and all should be encompassed in protection scope of the present invention.

Claims (4)

1. the breeding method of a silkworm grass, it is characterised in that comprise the steps: to be expelled to strain the abdomen body-surface of live body 5 silkworm in age, it is ensured that sterile liquid flows out;After inoculation, disinfection of silkworm body is placed in temperature be 15 ~ 20 DEG C, humidity be 50 ~ 70% the aseptic darkroom of constant temperature in carry out sending out a bacterium, after silkworm body ossifys, choose except downright bad silkworm body, intact silkworm body is carried out secondary sterilization, then loads in bottle and seal, humidity be 75 ~ 80%, illumination cultivates when being 2000 ~ 2500lx, photosensitive grow grass 35 ~ 45 days after gather, carry out drying prepared silkworm grass afterwards.
2. the breeding method of silkworm according to claim 1 grass, it is characterised in that the concentration of described strain is 200 ~ 300mg/L, and inoculum concentration is 1 ~ 2ml.
3. the breeding method of silkworm according to claim 1 grass, it is characterised in that the temperature sent out in bacterium process controls at 16 ~ 18 DEG C, and humid control is between 55 ~ 65%.
4. the breeding method according to the arbitrary described silkworm grass of claims 1 to 3, it is characterised in that the disinfectant solution adopted during disinfection of silkworm body is Methylpartricin Sodium Laurylsulfate, and its concentration is 0.5 ~ 0.7g/L.
CN201610097334.4A 2016-02-23 2016-02-23 Cordycepsmilitaris cultivation method Pending CN105706738A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610097334.4A CN105706738A (en) 2016-02-23 2016-02-23 Cordycepsmilitaris cultivation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610097334.4A CN105706738A (en) 2016-02-23 2016-02-23 Cordycepsmilitaris cultivation method

Publications (1)

Publication Number Publication Date
CN105706738A true CN105706738A (en) 2016-06-29

Family

ID=56156043

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610097334.4A Pending CN105706738A (en) 2016-02-23 2016-02-23 Cordycepsmilitaris cultivation method

Country Status (1)

Country Link
CN (1) CN105706738A (en)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101849477A (en) * 2009-03-30 2010-10-06 张天宝 Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof
CN102037856A (en) * 2009-10-16 2011-05-04 北京农业生物技术研究中心 Simple cordyceps militaris strain rejuvenation method
CN102440149A (en) * 2011-10-10 2012-05-09 江苏科技大学 Method for breeding cordyceps militaris by utilizing silkworm larvae
CN103430776A (en) * 2013-09-04 2013-12-11 鲁东大学 Silkworm pupa-worm grass culture method capable of improving yield and quality
CN103636408A (en) * 2013-12-18 2014-03-19 钱国琛 Factory-like production method of silkworm cordyceps
CN103798058A (en) * 2014-02-26 2014-05-21 山东省蚕业研究所 Method for cultivating aseptic cordyceps militaris
CN104429596A (en) * 2014-11-13 2015-03-25 北京天地同源生物科技有限公司 Method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms
CN104823714A (en) * 2015-05-04 2015-08-12 聂晶石 Large-scale cultivation method for cordyceps militaris
CN104871827A (en) * 2015-06-11 2015-09-02 孙建然 Artificially cultivated cordyceps sinensis and cultivation method thereof

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101849477A (en) * 2009-03-30 2010-10-06 张天宝 Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof
CN102037856A (en) * 2009-10-16 2011-05-04 北京农业生物技术研究中心 Simple cordyceps militaris strain rejuvenation method
CN102440149A (en) * 2011-10-10 2012-05-09 江苏科技大学 Method for breeding cordyceps militaris by utilizing silkworm larvae
CN103430776A (en) * 2013-09-04 2013-12-11 鲁东大学 Silkworm pupa-worm grass culture method capable of improving yield and quality
CN103636408A (en) * 2013-12-18 2014-03-19 钱国琛 Factory-like production method of silkworm cordyceps
CN103798058A (en) * 2014-02-26 2014-05-21 山东省蚕业研究所 Method for cultivating aseptic cordyceps militaris
CN104429596A (en) * 2014-11-13 2015-03-25 北京天地同源生物科技有限公司 Method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms
CN104823714A (en) * 2015-05-04 2015-08-12 聂晶石 Large-scale cultivation method for cordyceps militaris
CN104871827A (en) * 2015-06-11 2015-09-02 孙建然 Artificially cultivated cordyceps sinensis and cultivation method thereof

Similar Documents

Publication Publication Date Title
CN104164367B (en) Dried silkworm cordyceps militaris and culture method thereof
CN101463325A (en) Industrialized cultivation method for north aweto
CN102181389B (en) Method for breeding Bacillaceae with high spore yield
CN105660189A (en) Cultivation method for cordyceps militaris
CN105638244A (en) Silkworm cordyceps militaris culturing method
CN103858676A (en) Method for cultivating liquid cultures of cordyceps militaris
CN107896751A (en) A kind of preventing disease and pest method of strawberry seedling
CN107996404A (en) A kind of tendril-leaved fritillary bulb method for tissue culture
CN108373978B (en) Microbial preparation and application thereof
CN109302946A (en) A kind of cultivating method for edible fungi
CN103820122A (en) Microbial preparation for restoring and improving soil and degrading pesticide residue, and preparation method thereof
CN103329801A (en) Method for rooting culture of sugarcane tissue culture seedling
CN104938466B (en) A kind of environment-friendly type greenhouse disinfection method
CN103881930A (en) Solid culture medium and culture method of beauveria
CN105918132A (en) Rapid breeding method of clerodendrum trichotomum thunb
CN105706738A (en) Cordycepsmilitaris cultivation method
CN110550985A (en) method for producing organic fertilizer by fermenting shrimp shells with microorganisms
CN106069789B (en) One kind is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture
CN103849592A (en) Production method for streptomycete spores
CN103444440A (en) Lucid ganoderma production method
CN105670939A (en) Method for culturing cordyceps militaris mother strain
CN113455468A (en) Industrialized breeding method of sterile cordyceps sinensis host larvae
CN102487723B (en) Method for industrially producing bottle-cultivated or bag-cultivated lentinus giganteus berk without earthing
CN103858764A (en) Centipeda minima tissue culture rapid propagation medium and centipeda minima one-time seedling formation tissue culture rapid propagation method
CN104759445B (en) A kind of shield shell mould fermentation technique harmless treatment dreg method

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160629