CN103329801A - Method for rooting culture of sugarcane tissue culture seedling - Google Patents
Method for rooting culture of sugarcane tissue culture seedling Download PDFInfo
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- CN103329801A CN103329801A CN2013102458857A CN201310245885A CN103329801A CN 103329801 A CN103329801 A CN 103329801A CN 2013102458857 A CN2013102458857 A CN 2013102458857A CN 201310245885 A CN201310245885 A CN 201310245885A CN 103329801 A CN103329801 A CN 103329801A
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- sugarcane tissue
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Abstract
The invention discloses a method for the rooting culture of a sugarcane tissue culture seedling. The method comprises the following steps of: inoculating a multiplication sterile tissue culture seedling in a culture medium of MS (Murashige-Skoog), sodium hypochlorite with the concentration being 800-1200mg/L and 30g/L of white sugar, sealing, placing the mixture in a culture room with the temperature of 27+/-1 DEG C, carrying out the rooting culture by illuminating for 12-16h every day, so as to realize the purpose that a complete root system grows after 20-30 days. According to the method disclosed by the invention, the culture medium does not contains exogenous hormones and does not need to be subjected to high-pressure sterilization, carries out the inoculation without an ultraclean working platform, and is simple and rapid to operate and low in pollution rate; the rooting culture cost can be reduced by 40-60 percent; the cultured rooting seedling grows strongly.
Description
Technical field
The present invention relates to the Plant Tissue Breeding field, specifically is a kind of sugarcane tissue-culture seedling rooting cultured method.
Background technology
Plant Tissue Breeding has: save a large amount of space and time 1.; 2. incubation is not subjected to the influence of the external environment factor, and all can carry out all the year round; 3. three big advantages such as repeatability.Often extensively promoted and be applied to fields such as the breeding in a large number of virus-free seedling, mutation breeding, transgenosis, secondary metabolites production, artificial seed, and produced enormous benefits.But tissue culture technology has strict demand to gnotobasis, causes application cost to raise greatly.Numerous and diverse sport technique segment and the instrument of group training process, for example supplementary measures such as sterile working platform, Biohazard Safety Equipment or high-pressure sterilizing pot are all formulated around preventing from polluting.And the factor that influence is polluted is varied, as the kind of explant, and the season of drawing materials, time, top processing method, the kind of disinfectant, concentration, disinfecting time and sterilization method; Medium and vessel sterilization, the requirement of operating personnel, working environment, the work quality of superclean bench etc. all is closely related with pollution.Therefore, make that pollution control problem is very complicated, and cost dearly, become one of major reason of restriction group training industrialization, also make the tissue culture technology range of application be subjected to great restriction, be unfavorable in conventional agriculture is produced, promoting with universal.Therefore, simplify tissue culture technique, prevent from having polluted into indispensable groundwork in the group training research.
At present, sugarcane tissue culture propagating technology is still carried out in a traditional way.
Summary of the invention
The object of the present invention is to provide a kind of sugarcane tissue-culture seedling rooting cultured method, medium does not contain exogenous hormone, does not need autoclaving, and inoculation does not need by superclean bench, and simple to operate, quick, pollution rate is low, can reduce culture of rootage cost 40-60%; The seedling robust growth of taking root of cultivating.
The present invention achieves the above object by the following technical programs: a kind of sugarcane tissue-culture seedling rooting cultured method, comprise the steps: aseptic group of training of propagation seedling is inserted in the MS+ clorox 800-1200mg/L+ white sugar 30g/L medium, seal, place culturing room to carry out growing in culture of rootage 20-30 days complete root system.
The temperature of described culturing room is 27 ± 1 ℃, illumination every day 12-16 hour.
Described MS is that Murashige and Skoog were in disclosed plant tissue culture media in 1962;
Its chemical formula of described clorox is NaClO.
Described medium does not contain exogenous hormone, does not need autoclaving sterilization.
The technique effect that the present invention gives prominence to is:
The sugarcane tissue-culture seedling rooting need not add exogenous hormone, and medium does not need autoclaving, inoculation not to need by superclean bench, fundamentally simplification group training link, and simple to operate, quick, pollution rate is low, reduces culture of rootage cost 40-60%; The seedling robust growth of taking root of cultivating.
Embodiment
Below by specific embodiment technical scheme of the present invention is described further.
Embodiment 1
Sugarcane tissue-culture seedling rooting cultured method of the present invention comprises the steps:
1. culture vessel is prepared
Be basic container with existing group culture container, it is standby to rinse in the natural airing container in back moisture well;
2. medium preparation
Get running water preparation MS+ clorox 8000mg/L+ white sugar 30g/L liquid nutrient medium, be divided in the culture vessel by a certain amount of then, seal, stand-by.
3. culture of rootage
Group training seedling inserted to be placed in the culturing room in the above-mentioned medium cultivate, cultivate after 20-30 days, group is trained seedling can grow up to complete root system.
4. implementation result
Embodiment 2
Another example of sugarcane tissue-culture seedling rooting cultured method of the present invention comprises the steps:
1. culture vessel is prepared
Be basic container with existing group culture container, it is standby to rinse in the natural airing container in back moisture well;
2. medium preparation
After claiming 6-8 gram agar+200 ml tap waters are boiled by every liter of medium, preparation MS+ clorox 1200mg/L+ white sugar 30g/L medium is mixed thoroughly with running water, is divided in the culture vessel by a certain amount of before culture medium solidifying, seals, and is stand-by.
3. culture of rootage
Group training seedling inserted to be placed in the culturing room in the above-mentioned medium cultivate, cultivate after 20-30 days, group is trained seedling can grow up to complete root system.
4. implementation result
Claims (1)
1. sugarcane tissue-culture seedling rooting cultured method, it is characterized in that: will breed aseptic group of training seedling and insert in the MS+ clorox 800-1200mg/L+ white sugar 30g/L medium, seal, placing culturing room's temperature is 27 ± 1 ℃, and illumination every day carried out growing in culture of rootage 20-30 days complete root system in 12-16 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013102458857A CN103329801A (en) | 2013-06-20 | 2013-06-20 | Method for rooting culture of sugarcane tissue culture seedling |
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| CN2013102458857A CN103329801A (en) | 2013-06-20 | 2013-06-20 | Method for rooting culture of sugarcane tissue culture seedling |
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| CN103329801A true CN103329801A (en) | 2013-10-02 |
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| CN2013102458857A Pending CN103329801A (en) | 2013-06-20 | 2013-06-20 | Method for rooting culture of sugarcane tissue culture seedling |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273030A (en) * | 2014-10-11 | 2015-01-14 | 广西壮族自治区农业科学院甘蔗研究所 | Photoautotrophic rooting method of sugarcane test-tube plantlets |
| CN104885944A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for Saccharum officimarum.L |
| CN104920215A (en) * | 2015-06-08 | 2015-09-23 | 广西壮族自治区农业科学院甘蔗研究所 | Simplified sugarcane test-tube plantlet rooting and seedling method |
| CN108739369A (en) * | 2018-03-16 | 2018-11-06 | 福建农林大学 | A kind of observation sugar-cane tissue culture seedlings root growth model in real time |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628507A (en) * | 2004-04-21 | 2005-06-22 | 单文修 | Method for cultivating plant tissue under non-aseptic condition |
| CN102405842A (en) * | 2011-10-14 | 2012-04-11 | 福建农林大学 | Open type method for cultivating toxin-free seedlings of sugarcanes |
| CN102907328A (en) * | 2012-11-14 | 2013-02-06 | 中国热带农业科学院热带生物技术研究所 | Method for utilizing microelements to accelerate tissue culture seedling of sugarcane |
| CN103081808A (en) * | 2013-02-18 | 2013-05-08 | 广西壮族自治区农业科学院甘蔗研究所 | Sugarcane tissue culture and rapid propagation production method adopting ex vitro rooting |
-
2013
- 2013-06-20 CN CN2013102458857A patent/CN103329801A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628507A (en) * | 2004-04-21 | 2005-06-22 | 单文修 | Method for cultivating plant tissue under non-aseptic condition |
| CN102405842A (en) * | 2011-10-14 | 2012-04-11 | 福建农林大学 | Open type method for cultivating toxin-free seedlings of sugarcanes |
| CN102907328A (en) * | 2012-11-14 | 2013-02-06 | 中国热带农业科学院热带生物技术研究所 | Method for utilizing microelements to accelerate tissue culture seedling of sugarcane |
| CN103081808A (en) * | 2013-02-18 | 2013-05-08 | 广西壮族自治区农业科学院甘蔗研究所 | Sugarcane tissue culture and rapid propagation production method adopting ex vitro rooting |
Non-Patent Citations (2)
| Title |
|---|
| R.A.SAWANT: "Use of Sodium Hypochloriteas Media Sterilantin Sugarcane", 《SUGAR TECH》 * |
| 韦鹏霄: "甘蔗离体培养的胚性细胞团诱导与分化研究", 《广西大学学位论文》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273030A (en) * | 2014-10-11 | 2015-01-14 | 广西壮族自治区农业科学院甘蔗研究所 | Photoautotrophic rooting method of sugarcane test-tube plantlets |
| CN104273030B (en) * | 2014-10-11 | 2016-06-29 | 广西壮族自治区农业科学院甘蔗研究所 | Caulis Sacchari sinensis test tube Seedling photoautotrophy rooting method |
| CN104885944A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for Saccharum officimarum.L |
| CN104885944B (en) * | 2015-05-26 | 2017-01-11 | 福建农林大学 | Chemical disinfection tissue culture method for Saccharum officimarum.L |
| CN104920215A (en) * | 2015-06-08 | 2015-09-23 | 广西壮族自治区农业科学院甘蔗研究所 | Simplified sugarcane test-tube plantlet rooting and seedling method |
| CN108739369A (en) * | 2018-03-16 | 2018-11-06 | 福建农林大学 | A kind of observation sugar-cane tissue culture seedlings root growth model in real time |
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Application publication date: 20131002 |