CN106386445A - Rooting method of Hylotelephium erythrostictum by using water culture and cutting - Google Patents
Rooting method of Hylotelephium erythrostictum by using water culture and cutting Download PDFInfo
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- CN106386445A CN106386445A CN201611108211.2A CN201611108211A CN106386445A CN 106386445 A CN106386445 A CN 106386445A CN 201611108211 A CN201611108211 A CN 201611108211A CN 106386445 A CN106386445 A CN 106386445A
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- hylotelephium erythrostictum
- herba hylotelephii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The invention discloses a rooting method of Hylotelephium erythrostictum by using water culture and cutting, which comprises the steps: intercepting a stem section of Hylotelephium erythrostictum without plant diseases and insect pests, inserting the base of the stem portion into a rooting culture medium, and culturing at 18 to 25 DEG C for 1 to 3 weeks, wherein the rooting culture medium is a basic nutrient solution to which a plant growth regulator is added, the plant growth regulator comprises auxin analogue and a cytokinin analogue at a concentration ratio of 1:1, and the basic nutrient solution is a 50% Hoagland nutrient solution. The quick rooting method of Hylotelephium erythrostictum by using water culture and cutting greatly improves the rooting rate and root quantity of Hylotelephium erythrostictum by using water culture and cutting, and solves the key technical problems about rapid propagation of Hylotelephium erythrostictum and has great significance for rapid propagation and industrialized nursery and production of Hylotelephium erythrostictum.
Description
Technical field
The invention belongs to crassulaceae plants Rapid Rooting technical field, more particularly to a kind of life of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture
Method for root.
Background technology
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is Crassulaceae, Sedum perennial meat herbaceous plant, also known as magnificent Herba hylotelephii erythrosticti, Sedum spectabilis great Ye scape
It etc..The subterraneous stem of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is plump, and aerial stem of plant cluster is raw, sturdy and upright, and Herb becomes celadon, plant height 30-50cm, impeller
Raw or opposite, obovate are in meat, and have wave-formed mouth.The intensive such as flat-head shape of corymb, inflorescence footpath 10~13cm, flower is light powder
Color, also has white, aubergine, rose kind, is pattern species the most gorgeous in Crassulaceae.Hylotelephium erythrostictum (Miq.) H.Ohba is commonly used therefore in gardens
Herba hylotelephii erythrosticti, as coordinating other Flowers'Arrangement flower beds, flower border or planting in flakes and do bank protection cover plant, has in afforestation field
Wide application prospect.
Meanwhile, in recent years, heavy metal pollution of soil has become the serious environmental problem that the whole world faces.At present, China's agriculture
Field The Heavy Metal Contaminated Soil is serious, is shown according to First National Soil Pollution Investigation result, China's arable soil point position
Exceeding standard rate is 19.4%, wherein slightly, slightly, moderate and serious pollution point position ratio be respectively 13.7%, 2.8%, 1.8% and
1.1%;And from the point of view of the exceeded situation of pollutant, heavy metal cadmium point position exceeding standard rate is 7.0%.China is subject to heavy metal pollution now
About 25,000,000 hectares of agricultural land soil, up to 12,000,000 tons of grain contaminated by heavy metals every year, the direct economic loss causing surpasses
Cross 10,000,000,000 yuan.And Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti has stronger accumulation ability to heavy metal in soil cadmium, there is restoration of soil polluted by heavy metal
Potentiality, so as to be applied to the reparation tool of heavy-metal contaminated soil the features such as it is adaptable, growth is rapid, Biomass is big
Have that rehabilitation cost is relatively low, little to environmental perturbation, non-secondary pollution and the advantage such as sustainability is strong, have the work beautified the environment concurrently simultaneously
With, thus hold out broad prospects.
In view of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti has a extensive future, its seedling demand is also gradually increased.And Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti kind grain is small, broadcast
Kind of seedling-raising technique is loaded down with trivial details, and the cycle is longer and seedling is slow, produces frequently with Technique on Cuttage Propagation, and cuttage often due to
Take root slow, lead to Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti growth and breeding slower, affect its popularizing planting on a large scale.Therefore, how to solve Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti
A Rapid Rooting difficult problem, be the key technique of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Fast-propagation, this by for Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Fast-propagation and
Industrial seedling rearing produces significant.
Content of the invention
In view of this, it is contemplated that proposing a kind of method that crassulaceae plants Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture is taken root, to
Solve at least a portion technical problem present in above-mentioned prior art.
In order to solve above-mentioned technical problem, the present invention provides following technical proposals.
The present invention provides a kind of method that Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture is taken root, and it includes:Intercept the Hylotelephium erythrostictum (Miq.) H.Ohba scape of no disease and pests harm
It stem section, the base portion of described stem section is inserted in root culture liquid, cultivates 1~3 week under the conditions of 18-25 DEG C;
Wherein, described root culture liquid is the basic nutrient solution being added with plant growth regulator, and described plant gives birth to
It is 1 that long regulator includes concentration ratio:1 auxin analog thing similar with the basic element of cell division, described basic nutrient solution is
50% gram nutritional solution suddenly.
Wherein, described 50% suddenly gram nutritional solution be that this area is conventional described, it includes the acid of 2.5mmol/L tetra- liquid glauber salt
Calcium, 2.5mmol/L potassium chloride, 0.5mmol/L potassium dihydrogen phosphate, 0.5mmol/L Magnesium sulfate heptahydrate, 25 μm of ol/L boric acid, 2.25 μ
Mol/L manganese sulfate monohydrate, 1.9 μm of ol/L zinc sulphate heptahydrate, 0.15 μm of ol/L copper sulphate pentahydrate, 0.05mmol/L ammonium heptamolybdate and
5 μm of ol/L sodium iron ethylenediaminetetraacetate.
The described preferred Nafusaku of auxin analog, the described basic element of cell division is similar to the preferred butyric acid potassium of thing.
Described plant growth regulator preferably includes the Nafusaku of 0.2mg/L and the butyric acid potassium of 0.2mg/L.
Wherein, the length of described stem section preferably 8~10cm, and the upper end of described stem section is scabbled, the base portion of described stem section is cut
Become the inclined-plane shape of a hoof.
Described stem section inserts the length preferably 2~3cm in described root culture liquid.
As this area is conventional, described root culture liquid is usually contained in glass or plastic containers and need to be after sterilizing
Reuse it is preferable that described root culture liquid is contained in glass or plastic containers and after sterilized is used for cultivating described stem section,
And the vessel port of described glass or plastic containers wraps up shading with lower portion with light screening material, to guarantee inserting described stem section
After culture, the plant stem section some visible light of only external container, the equal black light of remainder.
The preferred masking foil of described light screening material or black cloth.
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti used in technical solution of the present invention has stronger accumulation ability to heavy metal in soil cadmium, utilizes
The features such as restoring cadmium polluted agricultural land soil of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti also has that input cost is low, extensive management, winter can prevent soil bareness,
Simultaneously because pattern is gorgeous, the florescence is longer, has preferable ornamental value and economic benefit, and therefore its reparative potential and popularization should
Have a extensive future, and the method that Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti water planting rapid cuttage proposed by the invention is taken root, substantially increase Hylotelephium erythrostictum (Miq.) H.Ohba scape
Take root speed and the root amount of its Water culture, solves the key technology difficulty of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Fast-propagation, to Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti
Fast-propagation and industrial seedling rearing production popularization are significant.
Brief description
Fig. 1 shows the method flow diagram that a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti water planting rapid cuttage according to embodiments of the present invention is taken root;
Fig. 2 is shown in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti under the conditions of different disposal and takes root the results contrast figure of situation.
Specific embodiment
For making the object, technical solutions and advantages of the present invention become more apparent, below in conjunction with specific embodiment, and reference
Accompanying drawing, the present invention is described in further detail.
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti has stronger accumulation ability to the heavy metal cadmium in soil, using the restoring cadmium polluted agriculture of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti
Field soil has that input cost is low, extensive management, winter can prevent the advantages such as soil bareness, simultaneously because pattern is gorgeous, the florescence
Longer, there is having a extensive future of preferable ornamental value and economic benefit, therefore its reparative potential and popularization and application.In view of Hylotelephium erythrostictum (Miq.) H.Ohba
The having a extensive future of Herba hylotelephii erythrosticti, its seedling demand is also gradually increased.And Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti kind grain is small, seeding and seedling raising technology is numerous
Trivial, the cycle is longer and seedling is slow, produces frequently with Technique on Cuttage Propagation, and cuttage, often due to taking root slow, is led
Cause Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti growth and breeding slower, affect its popularizing planting on a large scale.For this reason, present inventor probes into through substantial amounts of
Test and through practical proof repeatedly it is proposed that technical scheme, solve the technology making Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Rapid Rooting
A difficult problem.In the course of the study, inventor finds, carries out water planting root culture to Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti and cannot simply indiscriminately imitate being applied to it
The method of his plant, therefore, inventor has carried out bold innovation and practice, finally obtains the present invention.
The method that the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture of the present invention is taken root, including:Intercept the stem section of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti of no disease and pests harm,
The base portion of described stem section is inserted in root culture liquid, cultivates 1~3 week under the conditions of 18-25 DEG C;Wherein, described take root
Culture fluid is the basic nutrient solution being added with plant growth regulator, and it is 1 that described plant growth regulator includes concentration ratio:1
Auxin analog thing similar with the basic element of cell division, described basic nutrient solution is 50% gram nutritional solution suddenly.
Wherein, described 50% suddenly gram nutritional solution be that this area is conventional described, it includes the acid of 2.5mmol/L tetra- liquid glauber salt
Calcium, 2.5mmol/L potassium chloride, 0.5mmol/L potassium dihydrogen phosphate, 0.5mmol/L Magnesium sulfate heptahydrate, 25 μm of ol/L boric acid, 2.25 μ
Mol/L manganese sulfate monohydrate, 1.9 μm of ol/L zinc sulphate heptahydrate, 0.15 μm of ol/L copper sulphate pentahydrate, 0.05mmol/L ammonium heptamolybdate and
5 μm of ol/L sodium iron ethylenediaminetetraacetate.
Inventor, through substantial amounts of screening and practical proof repeatedly, finds that described auxin analog is naphthalene second
Sour sodium, it is butyric acid potassium that the described basic element of cell division is similar to thing, and it enables optimal rooting efficiency.Especially, work as Nafusaku
With the concentration of butyric acid potassium be better when 0.2mg/L.
In culture, the length of described stem section preferably intercepts as 8~10cm, and the upper end of described stem section scabble, described stem
The base portion of section is whittled into the inclined-plane shape of a hoof.
Described stem section inserts the length preferably 2~3cm in described root culture liquid.
As this area is conventional, described root culture liquid is usually contained in glass or plastic containers and need to be after sterilizing
Reuse it is preferable that described root culture liquid is contained in glass or plastic containers and after sterilized is used for cultivating described stem section,
And the vessel port of described glass or plastic containers is wrapped up with light screening material (such as can use masking foil or black cloth) with lower portion
Shading, to guarantee after inserting described stem section culture, the plant stem section some visible light of only external container, remainder is all not
Visible ray.
Name several specific embodiments, to better illustrate to the enforcement of the present invention and technique effect.
Embodiment 1
As shown in figure 1, the method flow diagram that in its display the present embodiment, Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti water planting rapid cuttage is taken root, now specifically
It is described as follows.
Using the test method of indoor water planting, basic nutrient solution is 50% gram nutritional solution suddenly, and it includes 2.5mmol/L
Four water-calcium nitrate, 2.5mmol/L potassium chloride, 0.5mmol/L potassium dihydrogen phosphate, 0.5mmol/L Magnesium sulfate heptahydrate, 25 μm of ol/L boron
Acid, 2.25 μm of ol/L manganese sulfate monohydrates, 1.9 μm of ol/L zinc sulphate heptahydrate, 0.15 μm of ol/L copper sulphate pentahydrate, 0.05mmol/L seven
Ammonium molybdate and 5 μm of ol/L sodium iron ethylenediaminetetraacetate.Plant growth regulator Nafusaku and Yin is added in basic nutrient solution
Diindyl potassium butyrate is so as to concentration is respectively 0.2mg/L and 0.2mg/L.
Specifically, configure basic nutrient solution first:It is respectively configured mother solution one (0.25mol/L four water-calcium nitrate), mother solution two
(0.05mol/L Magnesium sulfate heptahydrate, 0.25mol/L potassium chloride), mother solution three (0.025mol/L potassium dihydrogen phosphate), mother solution four
(0.01125mol/L manganese sulfate monohydrate, 0.0095mol/L zinc sulphate heptahydrate, 0.00075mol/L copper sulphate pentahydrate), mother solution five
(0.025mol/L boric acid, 0.05mol/L ammonium heptamolybdate) and mother solution six (0.0125mol/L sodium iron ethylenediaminetetraacetate).
Then, prepare plant growth regulator mother solution:For Nafusaku, Nafusaku 0.1g is taken to be dissolved in ultra-pure water,
Constant volume, to 1L, obtains the mother solution of 0.1g/L;For butyric acid potassium, take 0.0842g indolebutyric acid and 0.0232g potassium hydroxide molten
Yu Shuizhong, after fully dissolving, constant volume, to 1L, obtains the mother solution of 0.1g/L.
Afterwards, with each mother solution of basic nutrient solution and plant growth regulator mother liquor root culture liquid:Take basis respectively
Nutritional solution mother solution one arrives mother solution six 10mL, 10mL, 20mL, 0.2mL, 1mL and 0.4mL respectively, then takes plant growth regulator
Nafusaku mother solution and butyric acid potassium mother solution some (making its final concentration be respectively 0.2mg/L and 0.2mg/L), each component is filled
Divide and mix, be diluted to 1L, standby.
, for 1L transparent plastic basin as culture vessel, container outer wrapping masking foil shading is to keep away for the capacity of selection 18 × 12cm
Exempt to grow chlorella, with the 84 medicining liquid dipping 25-40min of 0.15-0.3%, clear water is rinsed well standby.With the poly- second that 1cm is thick
The cuttage plate that alkene cystosepiment is taken root as Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti water planting rapid cuttage, is cut into bottleneck size, and each cuttage plate makes a call to 4
0.5cm about hole be used for cuttage branch.
Choose the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti of annual robust growth no disease and pests harm, intercept stem section and be cut into 8-10cm length, stem section upper end is cut
Flat, lower end is whittled into the inclined-plane shape of a hoof, altogether 40 branches of clip.
The root culture liquid of 2/3 volume (about 600mL) is added, each cuttage plate inserts 4 Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrostictis in culture vessel
Branch, 1/3 in cutting insertion foam plate hole, is fixed with sponge, cystosepiment is put on bottle or in basin, makes cutting base portion 2-3cm's
Stem section can touch root culture liquid.Then cultivate under the conditions of 18-25 DEG C, the condition of rooting of continuous observation Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti.
Result shows, cultivates under conditions of the present embodiment, and the stem section of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti started to take root at one week, cultivates three
Zhou Hougen amount is very abundant, and after transfer cutting, healthy growth is rapid.
Comparative example 1~9
All with embodiment 1, difference is the plant growth regulator adding to other implementation processes of comparative example 1~9
Ratio different with concentration, specifically refer to table 1.
The plant growth regulator concentration of table 1 different experiments group
Wherein, comparative example 1 only provides basal nutrient material using Gram nutritional solution suddenly, without Nafusaku and Yin
Diindyl potassium butyrate.
After cultivating under identical condition of culture, the result of taking root of each comparative example and embodiment 1 is as shown in Fig. 2 wherein may be used
To find out, the amount of taking root of embodiment 1 is the abundantest, and root length is the longest, i.e. Nafusaku and butyric acid potassium ratio in root culture liquid
Example is with 1:1 is optimal, and adding concentration is optimal with 0.2mg/L and 0.2mg/L.Remaining each comparative example display Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti water planting life
, for the proportioning of plant growth regulator and concentration sensitive, small adjustment can appreciable impact result of the test for root.
Particular embodiments described above, has carried out detailed further to the purpose of the present invention, technical scheme and beneficial effect
Describing in detail bright it should be understood that the foregoing is only the specific embodiment of the present invention, being not limited to the present invention, all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement done etc., should be included in the protection of the present invention
Within the scope of.
Claims (8)
1. a kind of method that Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture is taken root is it is characterised in that include:Intercept the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti of no disease and pests harm
Stem section, the base portion of described stem section is inserted in root culture liquid, cultivates 1~3 week under the conditions of 18-25 DEG C;
Wherein, described root culture liquid is the basic nutrient solution being added with plant growth regulator, and described plant growing is adjusted
It is 1 that section agent includes concentration ratio:1 auxin analog thing similar with the basic element of cell division, described basic nutrient solution is for 50% suddenly
Gram nutritional solution.
2. the method that Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 1 is taken root it is characterised in that wherein, described 50%
Suddenly gram nutritional solution include 2.5mmol/L four water-calcium nitrate, 2.5mmol/L potassium chloride, 0.5mmol/L potassium dihydrogen phosphate,
0.5mmol/L Magnesium sulfate heptahydrate, 25 μm of ol/L boric acid, 2.25 μm of ol/L manganese sulfate monohydrates, 1.9 μm of ol/L zinc sulphate heptahydrate,
0.15 μm of ol/L copper sulphate pentahydrate, 0.05mmol/L ammonium heptamolybdate and 5 μm of ol/L sodium iron ethylenediaminetetraacetate.
3. Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 1 is taken root method is it is characterised in that described auxins
It is Nafusaku like thing, it is butyric acid potassium that the described basic element of cell division is similar to thing.
4. Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 1 is taken root method is it is characterised in that described plant growing
Regulator includes the Nafusaku of 0.2mg/L and the butyric acid potassium of 0.2mg/L.
5. the method that Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 1 is taken root it is characterised in that wherein, described stem section
Length be 8~10cm, and the upper end of described stem section is scabbled, the base portion of described stem section is whittled into the inclined-plane shape of a hoof.
6. the method that Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 1 is taken root is it is characterised in that described stem section inserts institute
Stating the length in root culture liquid is 2~3cm.
7. Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 1 is taken root method is it is characterised in that described root culture liquid
It is contained in glass or plastic containers and after sterilized and be used for cultivating described stem section, and the container of described glass or plastic containers
Mouth is wrapped up with light screening material with lower portion.
8. Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti Water culture according to claim 7 is taken root method is it is characterised in that described light screening material
For masking foil or black cloth.
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CN111149673A (en) * | 2020-02-24 | 2020-05-15 | 宿迁市博雅农业科技发展有限公司 | Method for promoting water culture cutting rooting of crassulaceae succulent plants in hazy moon |
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CN111374033A (en) * | 2020-04-22 | 2020-07-07 | 广西壮族自治区中国科学院广西植物研究所 | Water planting cutting seedling method for giant reed |
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