CN109042342A - A kind of method for tissue culture of Chunlan - Google Patents
A kind of method for tissue culture of Chunlan Download PDFInfo
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- CN109042342A CN109042342A CN201811296576.1A CN201811296576A CN109042342A CN 109042342 A CN109042342 A CN 109042342A CN 201811296576 A CN201811296576 A CN 201811296576A CN 109042342 A CN109042342 A CN 109042342A
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- culture
- rhizomes
- seedling
- chunlan
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of method for tissue culture of Chunlan, removing, Stem tip induction rhizomes, the proliferation of rhizomes, rhizomes differentiation budding, strong seedling culture and the seedling rooting culture of disinfection and stem apex including explant and etc., it goes to be cultivated using different culture mediums in Stem tip induction rhizomes, the proliferation of rhizomes, rhizomes differentiation budding, strong seedling culture and seedling rooting culture, meets nutrition and the hormonal requirement in each period.The invention can be obtained more Chunlan plant faster, be met the production requirement of Chunlan large-scale commercial applications by the way of vegetative propagation.
Description
Technical field
The present invention relates to plant biological fields, obtain plant, in particular to a kind of spring by the vegetative propagation of tissue cultures
Blue method for tissue culture.
Background technique
Chunlan, alias piece piece perfume (or spice), round trip flight swallow, cymbidium, grass element, mountain flower, originates in China, is mainly distributed on Zhejiang, Anhui, river
The ground such as south, Gansu, Sichuan, Yunnan are most colourful one kind of kind in Chinese cymbidium large family.Chunlan is that China's orchid family is planted
Most wide, the most abundant a kind of orchid of kind is distributed in object.Nowadays domestic to have formed one " the blue heat of state ", push orchid industry
It rapidly develops, also results in the excessive exploitation and serious destruction of orchid resource, cause Chunlan to have become endangered species, " sky-high price is blue
Flower " is reported in media frequently.Due to the deterioration of excessive excavation and environment, for Chunlan resource by serious destruction, conventional plant division is numerous
The excellent kind of breeding and popularization of cymbidium varieties are grown and are unfavorable for, not only the period is long for existing Chunlan method for tissue culture, and rhizomes
Bud Differentiation is few, and the big seedling rooting that bud grows up to is difficult, and high production cost, return rate are low.For these problems, the present invention provides a kind of spring
Blue rapid propagation method.
Summary of the invention
In view of the deficiencies of the prior art, the purpose of the present invention is to provide a kind of method for tissue culture of Chunlan, improve root
Shape stem breaks up the probability of budding, small seedling rooting.
The technical scheme adopted by the invention is as follows:
A kind of method for tissue culture of Chunlan, the removing of disinfection and stem apex including explant, Stem tip induction rhizomes, root
Proliferation, rhizomes differentiation budding, strong seedling culture and the seedling rooting culture of shape stem and etc.:
In the selection disinfection and the removing of stem apex of explant, the bud for taking Chunlan blade not open is explant, uses flowing water
The bud for rinsing Chunlan is carried out disinfection processing with alcohol, bleaching water, repeatedly with aseptic water washing, using different disappear is used for multiple times
The mode of toxic agent carries out disinfection processing to explant, it is ensured that the explant of acquisition is no thallus；Then outer blade is cut off, then
With mercuric chloride, sterile water process, sterile scalpel is finally used, strips out stem apex, the stem apex guaranteed will not be contaminated, after raising
The survival rate of phase culture.
The stem apex stripped is subjected to induction rhizomes culture, stem apex is inoculated into, precious No. 1+potato juice 500- is spent by the U.S.
The first culture medium training of 1500mg/L+ bananas juice 500-1500mg/L+ peptone 500-1500mg/L+ active carbon 1g/L composition
It supports, each component and proportion in this culture medium meet the nutritional need of Chunlan stem apex just, can get rhizomes after cultivating the several months.
Then rhizomes is seeded to by MS+6- benzyl aminoadenine 1-5mg/L+ methyl α-naphthyl acetate 0.1-5mg/L+ active carbon
Multiplying culture is carried out in the proliferated culture medium of the rhizomes of 1g/L composition, according to experimental result, in the training of this ingredient and ratio
It supports and is cultivated in base, a rhizomes can be proliferated 4-5 times, can get a large amount of rhizomes, facilitate the later period in the form of the increasing of index
Obtain Chunlan plant.
Next rhizomes is subjected to differentiation budding culture, rhizomes is seeded to by MS+6- benzyl aminoadenine 1-
Differentiation budding is carried out in the rhizomes differentiation budding culture medium of 5mg/L+ methyl α-naphthyl acetate 0.1-5mg/L+ kinetin 1-10mg/L composition
Culture, nutrition and hormone needed for the component and content that rhizomes breaks up in budding culture medium can satisfy rhizomes differentiation budding
Demand can preferably promote the differentiation of rhizomes, obtain budlet；According to experimental result, every rhizomes can differentiate 3-5
Budlet.
After rhizomes differentiates multiple budlets, then strong seedling culture is carried out, budlet is seeded to by MS+6- benzyl aminoadenine
It is trained in the strong seedling culture base of 1-5mg/L+ methyl α-naphthyl acetate 0.1-2mg/L+ kinetin 1-10mg/L+ bananas juice 500-1500mg/L composition
It supports, 6- benzyl aminoadenine, methyl α-naphthyl acetate, the kinetin for adding proper ratio in the medium can preferably promote cell point
It splits, adds a certain amount of nutriment, can preferably promote budlet differential growth, after cultivating a period of time, can obtain
Seedling.
The seedling of acquisition is subjected to seedling rooting culture, seedling is seeded to, precious No. 1+methyl α-naphthyl acetate 0.1-5mg/L+ is spent by the U.S.
Indolebutyric acid 1-5mg/L+ bananas juice 500-1500mg/L+ potato juice 500-1500mg/L+ peptone 500-1500mg/L+ is living
Property charcoal 1g/L composition seedling rooting culture medium in carry out seedling rooting culture, adding methyl α-naphthyl acetate and indolebutyric acid in the medium can be with
Preferably promote the induction long root of Chunlan seedling, can get the Chunlan seedling taken root after a period of time.
Preferably, first being impregnated 30-35 seconds, being used with the alcohol that concentration is 70%-75% in the selection disinfection of explant
It is rinsed 3-4 times when aseptic water washing, removes the alcohol remained on explant；Then using the bleaching water sterilization for diluting 10 times
It 10-15 minutes, is rinsed 3-4 times with when aseptic water washing, after outer blade is cut off, then uses concentration for 0.1%-0.2%'s
Mercuric chloride sterilizes 10-15 minutes, is rinsed 3-4 times with when aseptic water washing, aseptic paper suck dry moisture strips out unpolluted stem apex.
The size for paying attention to stem apex stripping, is advisable with the height of 0.4-0.8cm, and too big disinfection is not thorough, and later period culture is easy death；Too little Rong
Easily when cultivating in the later period, it is easy gradually Necrosis.
Preferably, being cultivated in the first culture medium 6 months or so time, preceding 30 in Stem tip induction rhizomes culture
Its stem apex is cultivated in the environment of complete darkness, prevents from starting stem apex Necrosis under strong light action when culture, later in light
Under the conditions of intensity 1000lux, daily illumination 12 hours, temperature is cultivates in 25 ± 1 DEG C of environment, foundation experimental result, and one
A stem apex can get 3-5 rhizomes.
Precious No. 1+potato juice 500-1500mg/L+ banana is spent by the U.S. preferably, being forwarded to the rhizomes of acquisition
Continue to cultivate in second culture medium of juice 500-1500mg/L+ active carbon 1g/L composition, root shape can be given in this culture medium
Stem provides more nutriments needed for it, allows the better growth and development of rhizomes；It is shown according to experimental result, when cultivating one section
Between after, can get more sturdy, the good rhizomes to grow fine.
Preferably, good rhizomes is forwarded to the proliferated culture medium of rhizomes in the Multiplying culture of rhizomes,
Daily illumination 12-14 hours, intensity of illumination 1000lux, 25 ± 1 DEG C of temperature, the time of culture 6 months or so can get a large amount of
Rhizomes, according to experimental result, a rhizomes can be proliferated 4-5 times, using good rhizomes progress Multiplying culture
Proliferation rate is greater than the proliferation rate for directlying adopt the rhizomes progress Multiplying culture of Stem tip induction acquisition.
Preferably, good rhizomes is seeded to rhizomes differentiation budding training when rhizomes breaks up budding culture
After supporting base, daily illumination 12 hours, intensity of illumination 1000lux, 25 ± 1 DEG C of temperature, the time of culture 6 months or so, rhizomes
Budlet is differentiated, a rhizomes can break up 3-5 budlet.
Preferably, budlet is seeded in strong seedling culture base when strong seedling culture, every bottle culture medium inoculated 15 clumps, totally 45
A bud daily illumination 12 hours, intensity of illumination 1000lux, 25 ± 1 DEG C of temperature, is cultivated 4 months, can finally obtain more than 20 plants
Seedling.
Preferably, height is used to carry out seedling rooting culture for the seedling of 3cm or more, seedling has higher rooting rate, according to
According to experimental analysis, the height of seedling will affect the rooting rate of seedling, and when the height of seedling is lower than 3cm, most seedling is not taken root, and is taken root
Rate be lower than 10%, and the height of seedling be higher than 3cm when, rooting rate be greater than 60%.The Chunlan seedling that will be taken root, after carrying out hardening treatment,
Transplanting cultivation is carried out, the survival rate of plantation is up to 95% or more.
The beneficial effects of the present invention are:
(1) present invention is improved and is passed using the growth coefficient for improving Chunlan of the technology high degree of Vegetative tissue culture
The division propagation of system is only capable of 3 buds of breeding for 1 year, it is difficult to meet this defect of the needs of large-scale production, and use the present invention
Method, breeding amount exponentially increases again, is able to satisfy Chunlan and commercially produces demand.
(2) present invention is disappeared in the disinfecting process of explant using a variety of disinfectants such as alcohol, bleaching water, mercuric chloride
Poison, and repeatedly rinsed using sterile water, it can be ensured that the stem apex stripped out is unpolluted, so that stem apex is with higher
Survival rate.
(3) present invention goes to cultivate stem apex in Stem tip induction rhizomes culture using two kinds of culture mediums, relative to
It goes to cultivate using a kind of culture medium, the growth result for the rhizomes that the present invention obtains is more ideal, there is more newborn points；And
The initial stage of the first culture medium culture cultivates 30 days in the environment of complete darkness, can significantly reduce the brown of stem apex
Become dead, improves the survival rate of stem apex.
(4) seedling is filled in the present invention and different culture mediums is respectively adopted in seedling rooting, first obtain higher seedling, then carry out seedling
Culture of rootage improves seedling rooting rate, and solution seedling is towards rhizomes wraparound, without taking root, the defects such as survival rate is low, and obtained root is raw
For seedling when transplanting culture in the later period, the survival rate of plantation may be up to 95%, can satisfy the demand of Chunlan large-scale commercial.
Specific embodiment
It, below will be with specific real to keep above-mentioned technical problem, technical solution and the advantage of the invention solved clearer
Example is applied to be described in detail.
The present invention relates to a kind of method for tissue culture of Chunlan, removing, stem apex including disinfection and stem apex to explant
Induction rhizomes, the proliferation of rhizomes, rhizomes differentiation budding, strong seedling culture and seedling rooting culture and etc. obtain the spring taken root
Lan Miao:
In explant when selecting disinfection and the removing of stem apex: the bud for taking Chunlan blade not open is explant, first with stream
Water rinses the budlet of Chunlan, some dust for being attached to budlet is removed, then impregnated 30-45 seconds with the alcohol of 70-75%, to explant
Body carries out the disinfection treatment of first time, then uses aseptic water washing 3-4 times, removes the alcohol remained on explant；Then it uses
It is that spreader-sticker is handled 10-15 minute that a drop dish washing liquid, which is simultaneously added, in the bleaching water of 10 times of dilution, carries out second disinfection, then with sterile
Water rinses repeatedly, cuts away outer blade；Again with the mercuric chloride of 0.1%-0.2% and add one drop dish washing liquid be spreader-sticker, disinfection treatment
It 10-15 minutes, sufficiently shakes, carries out third time disinfection, then use aseptic water washing 3-4 times, be placed on suck dry moisture on aseptic paper；
Sterile scalpel is finally used, unpolluted stem apex is stripped out.In stem apex stripping, the stem apex of the height of 0.4-0.8cm is preferably stripped,
The too big possible disinfection of stem apex is not thorough, and is easy contaminated death；Stem apex is too small, then is easy to be easy gradually brown stain when cultivating in the later period
Death, therefore the height for preferably stripping stem apex is 0.4-0.8cm.
In Stem tip induction rhizomes culture: the stem apex stripped being forwarded to and spends precious No. 1+potato juice 500- by the U.S.
First culture medium culture of 1500mg/L+ bananas juice 500-1500mg/L+ peptone 500-1500mg/L+ active carbon 1g/L composition
Middle culture, at first 30 days, stem apex was in the environment of complete darkness and cultivates, and it is brown under strong light action to prevent from starting stem apex when culture
Becoming dead, the first culture medium is gone under the illumination condition that intensity is 1000luxd later, daily illumination 12 hours, and temperature is 25 ±
1 DEG C, 6 months or so time is co-cultured, 3-5 rhizomes can be grown by being found through experiments that on a stem apex, then will be with
The stem apex of rhizomes is forwarded to the U.S. and spends precious No. 1+potato juice 500-1500mg/L+ bananas juice 500-1500mg/L+ active carbon
In the second culture medium of 1g/L, the more good good rhizomes of more sturdy, growing way can be obtained from experimental result discovery.
In the Multiplying culture of rhizomes: good rhizomes is inoculated by MS+6- benzyl aminoadenine 1-5mg/L+
In the proliferated culture medium of the rhizomes of methyl α-naphthyl acetate 0.1-5mg/L+ active carbon 1g/L composition, 11 root shapes of every bottle of culture medium inoculated
Stem, intensity be 1000lux illumination condition under, daily illumination 12 hours, temperature be 25 ± 1 DEG C, culture 6 months or so when
Between, it can get the very good rhizomes of a large amount of growth conditions from experimental result discovery, a rhizomes can be proliferated 4-5 times.
When rhizomes breaks up budding culture: rhizomes is inoculated by MS+6- benzyl aminoadenine 1-5mg/L+ naphthalene second
In the rhizomes differentiation budding culture medium of sour 0.1-5mg/L+ kinetin 1-10mg/L composition, daily illumination 12 hours, illumination is strong
1000lux is spent, 25 ± 1 DEG C of temperature, after cultivating 6 months or so time, experimental result finds that every rhizomes can differentiate
3-5 budlet
In strong seedling culture: the budlet differentiated by rhizomes is inoculated by MS+6- benzyl aminoadenine 1-5mg/L+
In the strong seedling culture base of methyl α-naphthyl acetate 0.1-2mg/L+ kinetin 1-10mg/L+ bananas juice 500-1500mg/L composition, every bottle of culture
Base is inoculated with 15 clumps, shares 45 buds, and daily illumination 12 hours, intensity of illumination 1000lux, 25 ± 1 DEG C of temperature, by 4 months
The incubation time of left and right wherein has more than 20 young plants from experimental result discovery, grows up to seedling.
In seedling rooting culture: will be forwarded to by strong seedling culture culture seedlings obtained and spend precious No. 1+methyl α-naphthyl acetate by the U.S.
0.1-5mg/L+ indolebutyric acid 1-5mg/L+ bananas juice 500-1500mg/L+ potato juice 500-1500mg/L+ peptone 500-
It is as can be seen from Table 1 3cm's or more preferably with height in the seedling rooting culture medium of 1500mg/L+ active carbon 1g/L composition
Seedling is put into seedling rooting culture medium culture, has 60% or more seedling and can bear 6 to 10 roots, and seedling is in good condition；And
Culture of rootage is carried out using 1-2cm or 1cm seedling below, the rooting rate of seedling is lower than 10%, most seedling not president's root, but
Blade can turn yellow when returning, and cultivated to the later period towards rhizomes direction, can not be taken root and seedling in good condition.
Table 1, influence of the height of seedling to seedling rooting
Note: each processing investigates 10 bottles, every bottle of 10 young plants of inoculation
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art can easily think of the change or the replacement in technical scope disclosed by the invention, should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (8)
1. a kind of method for tissue culture of Chunlan, characterized by the following steps:
(1) removing for selecting disinfection with stem apex of explant: the bud for taking Chunlan blade not open is explant, rinses the spring with flowing water
Blue bud is carried out disinfection processing with alcohol, bleaching water, repeatedly uses aseptic water washing, then cut off outer blade, then with liter
Mercury, sterile water process finally use sterile scalpel, strip out stem apex；
(2) Stem tip induction rhizomes culture: stem apex is inoculated into, precious No. 1+potato juice 500-1500mg/L+ banana is spent by the U.S.
It is cultivated the several months in first culture medium of juice 500-1500mg/L+ peptone 500-1500mg/L+ active carbon 1g/L composition, obtains root
Shape stem；
(3) Multiplying culture of rhizomes: rhizomes is seeded to by MS+6- benzyl aminoadenine 1-5mg/L+ methyl α-naphthyl acetate 0.1-
Multiplying culture is carried out in the proliferated culture medium of the rhizomes of 5mg/L+ active carbon 1g/L composition, a rhizomes is proliferated 4-5 times；
(4) rhizomes differentiation budding culture: rhizomes is seeded to by MS+6- benzyl aminoadenine 1-5mg/L+ methyl α-naphthyl acetate 0.1-
Differentiation budding culture, a rhizomes point are carried out in the rhizomes differentiation budding culture medium of 5mg/L+ kinetin 1-10mg/L composition
Change 3-5 bud；
(5) strong seedling culture culture: budlet is seeded to and is swashed by MS+6- benzyl aminoadenine 1-5mg/L+ methyl α-naphthyl acetate 0.1-2mg/L+
It is cultivated in the strong seedling culture base of therbligs 1-10mg/L+ bananas juice 500-1500mg/L composition, obtains seedling；
(6) seedling rooting culture: seedling is seeded to, precious No. 1+methyl α-naphthyl acetate 0.1-5mg/L+ indolebutyric acid 1-5mg/L+ is spent by the U.S.
The seedling of bananas juice 500-1500mg/L+ potato juice 500-1500mg/L+ peptone 500-1500mg/L+ active carbon 1g/L composition
Seedling rooting culture is carried out in root media, obtains the Chunlan seedling taken root.
2. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: sterilized in the selection of explant
When, it is first impregnated 30-35 seconds with the alcohol that concentration is 70%-75%, with aseptic water washing 3-4 times, then using 10 times of dilution
Bleaching water sterilization 10-15 minutes, with aseptic water washing 3-4 times, after outer blade is cut off, then uses concentration for 0.1%-
0.2% mercuric chloride sterilizes 10-15 minutes, is rinsed 3-4 times with when aseptic water washing, aseptic paper suck dry moisture, finally strips out height
For the stem apex of 0.4-0.8cm.
3. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: in the Fiber differentiation of rhizomes
When, it is cultivated 6 months in the first culture medium, preceding 30 days stem apexs are cultivated in the environment of complete darkness, and daily illumination 12 is small later
When, intensity of illumination 1000lux, 25 ± 1 DEG C of temperature.
4. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: the root for obtaining Stem tip induction
Shape stem, which is forwarded to, spends precious No. 1+potato juice 500-1500mg/L+ bananas juice 500-1500mg/L+ active carbon 1g/L group by the U.S.
At the second culture medium in continue to cultivate, obtain good rhizomes.
5. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: in the Multiplying culture of rhizomes
When, rhizomes is transferred in the proliferated culture medium of rhizomes, daily illumination 12-14 hours, intensity of illumination 1000lux, temperature
It 25 ± 1 DEG C, cultivates 6 months.
6. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: break up budding training in rhizomes
Support when, by rhizomes be seeded to rhizomes differentiation budding culture medium, daily illumination 12 hours, intensity of illumination 1000lux, temperature 25
It ± 1 DEG C, cultivates 6 months, differentiates budlet.
7. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: when strong seedling culture, by budlet
It is seeded in strong seedling culture base, daily illumination 12 hours, intensity of illumination 1000lux, 25 ± 1 DEG C of temperature, cultivates 4 months, obtain
Seedling.
8. a kind of method for tissue culture of Chunlan according to claim 1, it is characterised in that: use height for 3cm or more
Seedling carry out seedling rooting culture.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112335545A (en) * | 2020-11-05 | 2021-02-09 | 贵州大学 | Culture medium for cymbidium rhizome tissue culture and tissue culture method |
| CN115316270A (en) * | 2022-07-12 | 2022-11-11 | 北京农业职业学院(中国共产党北京市委员会农村工作委员会党校) | Tissue culture induction method for cymbidium sinense |
| CN115669540A (en) * | 2022-09-29 | 2023-02-03 | 华南农业大学 | Cultivation method of orchid seedlings without endophyte pollution |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07107871A (en) * | 1993-10-08 | 1995-04-25 | Nakano Vinegar Co Ltd | Culture of orchidales plant |
| CN101822220A (en) * | 2010-05-11 | 2010-09-08 | 浙江大学 | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii |
| CN103999776A (en) * | 2014-06-16 | 2014-08-27 | 南京工业大学大丰海洋产业研究院 | Orchid rapid-propagation culture medium formula |
| KR20150103879A (en) * | 2014-03-04 | 2015-09-14 | 고려대학교 산학협력단 | Producing method of orchid seedlings |
| CN106035090A (en) * | 2016-07-07 | 2016-10-26 | 武汉生物工程学院 | Cymbidium rhizome tissue culture rapid propagation method |
| CN107750949A (en) * | 2015-07-22 | 2018-03-06 | 浙江传化生物技术有限公司 | The method that hybrid cymbidium seedling is efficiently bred using stem-tip tissue |
-
2018
- 2018-11-01 CN CN201811296576.1A patent/CN109042342A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07107871A (en) * | 1993-10-08 | 1995-04-25 | Nakano Vinegar Co Ltd | Culture of orchidales plant |
| CN101822220A (en) * | 2010-05-11 | 2010-09-08 | 浙江大学 | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii |
| KR20150103879A (en) * | 2014-03-04 | 2015-09-14 | 고려대학교 산학협력단 | Producing method of orchid seedlings |
| CN103999776A (en) * | 2014-06-16 | 2014-08-27 | 南京工业大学大丰海洋产业研究院 | Orchid rapid-propagation culture medium formula |
| CN107750949A (en) * | 2015-07-22 | 2018-03-06 | 浙江传化生物技术有限公司 | The method that hybrid cymbidium seedling is efficiently bred using stem-tip tissue |
| CN106035090A (en) * | 2016-07-07 | 2016-10-26 | 武汉生物工程学院 | Cymbidium rhizome tissue culture rapid propagation method |
Non-Patent Citations (1)
| Title |
|---|
| 李洪林 杨波: "春兰根状茎离体培养与快速繁殖(简报)", 《亚热带植物科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112335545A (en) * | 2020-11-05 | 2021-02-09 | 贵州大学 | Culture medium for cymbidium rhizome tissue culture and tissue culture method |
| CN115316270A (en) * | 2022-07-12 | 2022-11-11 | 北京农业职业学院(中国共产党北京市委员会农村工作委员会党校) | Tissue culture induction method for cymbidium sinense |
| CN115669540A (en) * | 2022-09-29 | 2023-02-03 | 华南农业大学 | Cultivation method of orchid seedlings without endophyte pollution |
| CN115669540B (en) * | 2022-09-29 | 2023-11-10 | 华南农业大学 | Cultivation method of orchid seedlings free of endophyte pollution |
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Application publication date: 20181221 |