CN101124892B - Cymbidium edaphic orchids seed aseptic seeding growing seedlings method - Google Patents
Cymbidium edaphic orchids seed aseptic seeding growing seedlings method Download PDFInfo
- Publication number
- CN101124892B CN101124892B CN2007100662308A CN200710066230A CN101124892B CN 101124892 B CN101124892 B CN 101124892B CN 2007100662308 A CN2007100662308 A CN 2007100662308A CN 200710066230 A CN200710066230 A CN 200710066230A CN 101124892 B CN101124892 B CN 101124892B
- Authority
- CN
- China
- Prior art keywords
- cymbidium
- aseptic
- seed
- edaphic
- orchids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method of the aseptic seeding and culturing of the cymbidium aseptic seed, comprising the steps of aseptic seeding, aseptic seedling culturing and seedling hardening, which is characterized in that after the aseptic seeding of the seed, a low temperature and darkness pre-culture step is added. The invention aims at enabling the cymbidium aseptic seed to complete the after ripening process and to promote germination by using the low temperature and dark condition and simulating the proterozonic environment of the cymbidium aseptic seed. Compared with the prior art, the invention improves the average bourgeon rate of the aseptic seeding of the cymbidium aseptic seed by over 10 times and has the advantages of simpleness and practicality, short seed culturing period and convenience for a large-scale production and so on, and the invention lays a firm basis for the novel variety selection of the cymbidium aseptic crossbreed.
Description
Technical field
The present invention relates to a kind of method of cymbidium edaphic orchids seed aseptic seeding growing seedlings.
Background technology
Carry according to " China blue platymiscium " secretary that Liu Zhongjian, Chen Xinqi etc. writes, the whole world has 68 kinds of Cymbidium (Cymbidium) plants approximately, according to life habit give birth to with being divided into, grow nonparasitically upon another plant, saprophytic three classes.Wherein saprophytic Research on kinds is few, both at home and abroad the tissue culture technique of the kinds of growing nonparasitically upon another plant such as Cymbidium hookerianum var. Lowianum (Rchb.f.)Y.S.wu et S.C.chen, Cymbidium hookerianum, cymbidium mastersii, yellow cicada orchid is studied early, and the aseptic seeding growing seedlings technology is comparative maturity also.Ground non-hibernating eggs classes such as lotus lobe orchid, bean cotyledon orchid, Chunlan, spring sword are used conventional Cymbidium epiphytic orchid aseptically sowing seeds technology, after planting seed almost can not be sprouted, the researcher has carried out some improvement to the cymbidium edaphic orchids seed aseptic seeding technology both at home and abroad, comprise seed is carried out ultrasonic oscillation, NaOH or KOH soaks, cuts to break in the seed coat etc. and handle the back inoculation, improve culture medium prescription, optimize condition of culture etc., but the also normal less than 1% even lower of seed germination rate.
Summary of the invention
The objective of the invention is to solve the low problem of present cymbidium edaphic orchids seed aseptic seeding germination rate, a kind of method that improves the cymbidium edaphic orchids seed aseptic seeding germination rate is provided.
The present invention is achieved in that in the process of conventional cymbidium edaphic orchids seed aseptic seeding growing seedlings, comprise that aseptic seeding, aseptic seedling are cultivated, the hardening step, behind aseptically sowing seeds, increase the dark pre-incubation step of low temperature, purpose is to imitate cymbidium edaphic orchids Proterozoic environment, utilizes the low temperature dark to allow cymbidium edaphic orchids seed finish the after-ripening process to promote sprouting.Particular content of the present invention is:
(1) aseptic seeding: take 6～9 maturations (definite from the time of the ripe cracking of capsule of pollinating) and still uncracked capsule according to each kind, cut residual perianth, gynostemium and long carpopodium, the surface is clean with 75% alcohol wipe, changes on the superclean bench, with 0.1%HgCl
2Middle soaking disinfection 15～20 minutes, sterile water wash 4～5 times is cut capsule then open and is taken out seed, broadcast sowing on the sowing medium, culture medium prescription is 1/4MS (a large amount of, trace element all changes 1/4 of MS medium into, and all the other components unchanged are together following)+6-BA 0.1～1mgL
-1+ NAA 1～5mgL
-1+ active carbon 2gL
-1+ coconut palm breast 100mLL
-1
(2) the pre-cultivation: after planting seed is placed under the low temperature dark condition together with culture vessel and cultivate in advance, pre-cultivation temperature is 0～10 ℃, and the time is 2～6 months;
(3) aseptic seedling is cultivated: after finishing pre-cultivation, seed is moved into the illumination cultivation chamber together with culture vessel cultivates, treat that seed germination forms root-like stock after, proliferated culture medium is gone in switching, make the root-like stock fast breeding, culture medium prescription is 1/4MS+6-BA 0.1～0.5mgL
-1+ NAA 0.5～2mgL
-1+ active carbon 2gL
-1, the root-like stock of in batches selecting robust growth changes differential medium over to, and bud is broken up in a large number, and culture medium prescription is 1/4MS+6-BA2～5mgL
-1+ NAA 0.5～1mgL
-1, changing the seedling that differentiates over to the strong plantlets and rootage medium then, culture medium prescription is 1/4MS+6-BA 0.1～0.5mgL
-1+ NAA1～4mgL
-1+ banana puree 50～150gL
-1, all medium pHs are 5.4～5.8, the illumination cultivation room temperature is 22 ± 3 ℃, intensity of illumination 25～30 μ molm
-2S
-1, light application time 14hd
-1
(4) hardening: well-grown aseptic seedling transferred in 15～25 ℃ the hardening canopy and cultivate after 15 days, open bottle cap gradually, transition 2～3 days, take out tissue cultivating seedling then, clean medium, with sphagna parcel root, 10～15 strains are one clump, close and plant in 9cm * 9cm (bore * height) nutritive cube, the initial stage is noted the shading heat and moisture preserving, can be transplanted to after 5 months in the common orchid cultivation matrix.
Method provided by the present invention makes the average germination rate of cymbidium edaphic orchids seed aseptic seeding improve more than 10 times than prior art; increased substantially the efficient of cymbidium edaphic orchids seeding and seedling raising; the effective ways that found a kind of seedling to cultivate; have simple to operate, growing-seedling period short, practical, be beneficial to advantages such as large-scale production, and take a firm foundation for cymbidium edaphic orchids selection cross new varieties.
Embodiment
Embodiment 1:
(1) sowing: take 7 months really blue capsules of lotus lobe in age, cut residual perianth, gynostemium and long carpopodium, the surface is clean with 75% alcohol wipe, changes on the superclean bench, with 0.1%HgCl
2In soaking disinfection 15 minutes, sterile water wash 5 times is cut capsule then open and is taken out seed, broadcast sowing on the sowing medium, culture medium prescription be 1/4MS (a large amount of, trace element all changes 1/4 of MS medium into, all the other components unchanged, down with)+6-BA0.1mgL
-1+ NAA 3mgL
-1+ active carbon 2gL
-1+ coconut palm breast 100mLL
-1
(2) the pre-cultivation: after planting seed is placed pre-the cultivation 3 months under 4 ℃ of constant temperature dark conditions together with culture vessel;
(3) aseptic seedling is cultivated: after finishing pre-cultivation, again seed is moved into the illumination cultivation chamber together with culture vessel and cultivate, seed is sprouted successively after 3 months, treat seed germination form root-like stock after switching go into proliferated culture medium, make the root-like stock fast breeding, culture medium prescription is 1/4MS+6-BA 0.25mgL
-1+ NAA 2mgL
-1+ active carbon 2gL
-1, the root-like stock of in batches selecting robust growth changes differential medium over to, and bud is broken up in a large number, and culture medium prescription is 1/4MS+6-BA4 mgL
-1+ NAA 1mgL
-1, changing the seedling that differentiates over to the strong plantlets and rootage medium then, culture medium prescription is 1/4MS+6-BA 0.1mgL
-1+ NAA 1mgL
-1+ banana puree 150gL
-1, all medium pHs are 5.4～5.8, the illumination cultivation room temperature is 22 ± 3 ℃, intensity of illumination 25～30 μ molm
-2S
-1, light application time 14hd
-1, sow that the sampling statistics seed germination rate is 12.7% after 1 year;
(4) bottle outlet hardening: well-grown aseptic seedling transferred in 20～25 ℃ the hardening canopy and cultivate after 15 days, open bottle cap gradually, tissue cultivating seedling is taken out in transition 2～3 days then, cleans medium, with sphagna parcel root, 10～15 strains are one clump, close and plant in 9cm * 9cm (bore * height) nutritive cube, and the initial stage is noted the shading heat and moisture preserving, can be transplanted to after 5 months in the common orchid cultivation matrix, the hardening survival rate is 95%.
Embodiment 2:
Take 6 months really blue capsules of bean cotyledon in age, 2～8 ℃ of natural alternating temperature dark conditions of aseptically sowing seeds postposition were cultivated 5 months down, and other step is undertaken by the method for embodiment 1, and the sampling statistics seed germination rate is 25.5%.
Embodiment 3:
Take 6.5 months really Chunlan in age * spring sword hybridization capsules, the rearmounted 7 ℃ of constant temperature dark conditions of planting seed were cultivated 4 months down, and other step is undertaken by the method for embodiment 1, and the sampling statistics seed germination rate is 21.4%.
Claims (2)
1. cymbidium edaphic orchids seed aseptic seeding growing seedlings method, comprise that aseptic seeding, aseptic seedling are cultivated, the hardening step, the kind of cymbidium edaphic orchids is lotus lobe orchid, bean cotyledon orchid, Chunlan, spring sword, it is characterized in that increasing the dark pre-incubation step of low temperature behind the aseptically sowing seeds, the dark pre-cultivation temperature of low temperature is 0～10 ℃, and the time is 2～6 months; The aseptic seeding culture medium prescription is 1/4MS+6-BA 0.1～1mgL
-1+ NAA 1～5mgL
-1+ active carbon 2gL
-1+ coconut palm breast 100mLL
-1Described 1/4MS medium is: a large amount of, trace element all changes 1/4 of MS medium into, and all the other compositions are constant.
2. according to the method for cymbidium edaphic orchids seed aseptic seeding growing seedlings described in the claim 1, it is characterized in that adopting and grow in the capsule of 6～9 maturations seed and carry out aseptic seeding and cultivate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100662308A CN101124892B (en) | 2007-09-25 | 2007-09-25 | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100662308A CN101124892B (en) | 2007-09-25 | 2007-09-25 | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101124892A CN101124892A (en) | 2008-02-20 |
| CN101124892B true CN101124892B (en) | 2010-11-10 |
Family
ID=39093097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100662308A Expired - Fee Related CN101124892B (en) | 2007-09-25 | 2007-09-25 | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101124892B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663070B (en) * | 2015-03-06 | 2016-03-30 | 广西壮族自治区农业科学院花卉研究所 | A kind of method promoting Chinese cymbidium seed germination |
| CN105706900B (en) * | 2016-01-26 | 2021-03-12 | 福建省农业科学院作物研究所 | Sterile seeding and seedling raising method for hybrid orchid and Tibet cymbidium hybrid seeds |
| CN106472072A (en) * | 2016-09-28 | 2017-03-08 | 合肥沁牧生态农业有限公司 | A kind of seeding growing seedlings method of kaffir lily |
| CN107493916A (en) * | 2017-09-25 | 2017-12-22 | 贵州大学 | A kind of hardening off method of bamboo shoot orchid tissue culture seedling |
| CN109258377B (en) * | 2018-11-14 | 2022-05-17 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Method for creating germplasm of watercress |
| CN114223545B (en) * | 2022-01-12 | 2022-08-30 | 中国科学院昆明植物研究所 | Culture medium for improving germination rate of Pleione bulbocodioides distant hybrid seeds and application and cultivation method thereof |
| CN115589944A (en) * | 2022-08-25 | 2023-01-13 | 云南省农业科学院花卉研究所（Cn） | Method for culturing cymbidium tortisepalum plants on large scale |
-
2007
- 2007-09-25 CN CN2007100662308A patent/CN101124892B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 杨宁生等.蕙兰（CYMBIDIUM FABERI ROLFE）种子无菌培养的研究.江西科学12 2.1994,12(2),80-84. |
| 杨宁生等.蕙兰(CYMBIDIUM FABERI ROLFE)种子无菌培养的研究.江西科学12 2.1994,12(2),80-84. * |
| 章玉平.兰花种子繁殖技术概况.中国花卉园艺 4.2003,(4),22-24. |
| 章玉平.兰花种子繁殖技术概况.中国花卉园艺 4.2003,(4),22-24. * |
| 陈光禄等.地生兰未成熟种子无菌发芽的研究.福建农业科技 3.1983,(3),27-29. |
| 陈光禄等.地生兰未成熟种子无菌发芽的研究.福建农业科技 3.1983,(3),27-29. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101124892A (en) | 2008-02-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101258835B (en) | Fast reproducing method for high quality seedling of dendrobium officinale | |
| CN101983554B (en) | Reproduction method of Chinese cymbidium seedlings | |
| CN100444722C (en) | Cyrtopterin tissue culturing method and fast reproduction thereof | |
| CN105340747A (en) | Asexual rapid propagation method for radix glycyrrhizae | |
| CN105706900B (en) | Sterile seeding and seedling raising method for hybrid orchid and Tibet cymbidium hybrid seeds | |
| CN101822220B (en) | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii | |
| CN101124892B (en) | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method | |
| CN101926259B (en) | Orchid germchit propagating method | |
| CN105104207A (en) | Method for obtaining regenerated plants of stevia rebaudiana Bertoni | |
| CN103704130A (en) | Chinese orchid and cymbidium hybridum hybrid seedling raising method | |
| CN106417011A (en) | Wild bletilla striata tissue culture rapid propagation method | |
| CN102090327A (en) | Method for quickly breeding Akebia trifoliata Koidz fruit seedling in test tube | |
| CN104429962A (en) | Cultivation method of dendrobium nobile tissue culture seedlings | |
| CN102613083A (en) | North American redwood tissue cultivation method | |
| CN104542283A (en) | Efficient angelica dahurica seed reproduction and seedling cultivation system | |
| CN106386488A (en) | Method for improving seed germination rate of large flower type paphiopedilum and cultivation method of large flower type paphiopedilum | |
| CN108575759B (en) | A method of bletilla striata tissue-culturing rapid propagation is carried out using carbon nanotube | |
| CN102239806B (en) | Artificial cultivation and seedling industrial production method of dendrobium nobile polyploid compound | |
| CN101622922B (en) | Symbiotic germination method of Chinese cymbidium hybrid seeds and bacteria | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN108243959A (en) | It is a kind of using yellow fine strain of millet wood stem section as the highly efficient regeneration method of explant | |
| CN100358413C (en) | Cymbidium plant seed asepsis sprouting and plant cultivating method | |
| CN106857258A (en) | A kind of quick breeding method for tissue culture blue with leaf pocket | |
| CN103975855A (en) | Haploid breeding method of dendrobium candidum | |
| CN104604390B (en) | A kind of method of podocarpus nagi seed sprouting and rooting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101110 Termination date: 20170925 |