CN104663070B - A kind of method promoting Chinese cymbidium seed germination - Google Patents
A kind of method promoting Chinese cymbidium seed germination Download PDFInfo
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- CN104663070B CN104663070B CN201510099892.XA CN201510099892A CN104663070B CN 104663070 B CN104663070 B CN 104663070B CN 201510099892 A CN201510099892 A CN 201510099892A CN 104663070 B CN104663070 B CN 104663070B
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- chinese cymbidium
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000732800 Cymbidium Species 0.000 title claims abstract description 28
- 230000007226 seed germination Effects 0.000 title claims abstract description 10
- 230000001737 promoting effect Effects 0.000 title abstract description 5
- 238000005406 washing Methods 0.000 claims description 12
- 239000002775 capsule Substances 0.000 claims description 8
- 239000012869 germination medium Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 235000020415 coconut juice Nutrition 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 230000035784 germination Effects 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract description 2
- 241000233855 Orchidaceae Species 0.000 description 6
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 2
- 241000173252 Cymbidium sinense Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000028446 budding cell bud growth Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of method promoting Chinese cymbidium seed germination, it is characterized in that, the method comprises the following steps: seed selection, the surface of the seed process, seed soaking and sprouting are cultivated.Method of the present invention has the effect of sprout time and raising germination rate ahead of time for Chinese cymbidium seed.
Description
Technical field
The present invention relates to technical field of flower cultivation, what be specifically related to is a kind of method promoting Chinese cymbidium seed germination.
Background technology
Chinese cymbidium (Cymbidiumsinense) is the Terrestrial orchid that a kind of ornamental value is higher.Blooms around the Spring Festival because mostly being, therefore also known as report township orchid, be one of representational kind of most in state orchid, be domesticly mainly distributed in the ground such as Anhui, Jiangxi, Fujian, Guangdong, Guangxi, Hainan, Yunnan, Hong Kong, Taiwan.The same with other orchids; due to habitat destruction with excessively excavate; wild Chinese cymbidium is endangered; belong to the object of protection in " wild animals and plants Convention on International Trade in Endangered Species " (ConventiononInternationalTradeinEndangeredspeciesofWildf aunaandFlora, CITES) annex I and be prohibited trade.
Chinese cymbidium is the same with other orchids, and embryo development is complete, and without endosperm, seed is extremely difficult under field conditions (factors) to be sprouted, and germination rate is low, and sprout time is long especially, generally needs individual month of 8-12 or longer time to sprout.Chinese cymbidium often adopts offshoot to breed, but due to its tiller few (3-4 bud/strain in 1 year), bud growth rate is slow, significantly limit the exploitation of high-quality Chinese cymbidium resource.And by animal nutrition to orchid non-symbiosis germination, effectively can improve the germination rate of seed and shorten sprout time, being conducive to seeling industry obtains successfully, can produce good economic benefit.Therefore, promote that the non-symbiosis germination time of Chinese cymbidium seed and germination rate can reach the object of producing seedling fast by biotechnology.
Summary of the invention
The object of this invention is to provide a kind of method promoting Chinese cymbidium seed germination, it is characterized in that, the method comprises the following steps: seed selection, the surface of the seed process, seed soaking and sprouting are cultivated.Method of the present invention has the effect of sprout time and raising germination rate ahead of time for Chinese cymbidium seed.
Technical scheme provided by the present invention is:
Promote a method for Chinese cymbidium seed germination, it is characterized in that, the method comprises the following steps:
(1) seed is selected: the Chinese cymbidium capsule choosing about 210d kind age;
(2) the surface of the seed process; Chinese cymbidium capsule after surface cleaning, is placed in superclean bench, after anhydrous filter paper blots surface moisture after plucking.After washing aseptic after the alcohol-pickled 5min of 75% 3 times, then after the mercury chloride surface sterilizing 10min using 0.1%, after aseptic washing 5-8 time;
(3) soak seed: capsule is cut open in the tea-bag sealed that Chinese cymbidium seed threading sterilization treatment is crossed; Then 10%H is adopted
2o
2aseptic washing 3 times after process 10min, 6-BA soaks 15min;
(4) sprout cultivation: the Chinese cymbidium seed after process is made the aqueous solution and sowed uniformly on germination medium, continues under being transposed to illumination after dark treatment 15d, cultivation temperature 25 DEG C, cultivate light application time 12h/d.
As preferably, germination medium described in step (3) is 1/2MS+0.05mg/LNAA+200mg/L Coconut Juice+20g/L sucrose+4g/L agar.
As preferably, the pH of described germination medium is 5.4-5.8.
Compared with prior art, the beneficial effect that the present invention has is:
Method of the present invention has the effect of sprout time and raising germination rate ahead of time for Chinese cymbidium seed.
Embodiment
Below in conjunction with specific embodiment, the present invention is further detailed explanation.
embodiment 1: distinct methods compares the sprout time of Chinese cymbidium seed and germination rate
(1) experimental procedure
1., after Chinese cymbidium capsule is plucked, after surface cleaning, be placed in superclean bench, after anhydrous filter paper blots surface moisture.After washing aseptic after the alcohol-pickled 5min of 75% 3 times, then after the mercury chloride surface sterilizing 10min using 0.1%, after aseptic washing 5-8 time;
2. capsule is cut open and be divided into 4 parts, in the tea-bag sealed that threading sterilization treatment is crossed.Process by the following method:
A: space management;
Aseptic washing 3-5 time after B:0.1NHCL process 10min, 6-BA soaks 15min;
C:10%H
2o
2aseptic washing 3-5 time after process 10min, 6-BA soaks 15min;
Aseptic washing 3-5 time after D:10%NaCLO process 10min, 6-BA soaks 15min;
E:6-BA soaks 15min;
3. the Chinese cymbidium seed after process is made the aqueous solution and is sowed (medium: 1/2MS+0.05mg/LNAA+200ml/L Coconut Juice+20g/L sucrose+4g/L agar on germination medium uniformly; PH:5.4-5.8), continue under being transposed to illumination after dark treatment 15d, cultivation temperature 25 DEG C, incubation time 12h/d;
4. the seed germination time, namely planting seed is to starting the time of sprouting in sky (d).Germination rate statistics is the germination rate of seed to 180d from planting seed.Formula is as follows:
Seed germination rate=(son sum is planted experimentally in the germination seed number/confession in unit are) × 100%.
(2) experimental result
The seed germination time of table 1 different disposal and germination rate
As shown in Table 1, C process (i.e. 10%H
2o
2aseptic washing 3 times after process 10min, 6-BA soaks 15min) after the sprout time of Chinese cymbidium seed can be advanced to about 30d, germination rate can reach 87%.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (1)
1. promote a method for Chinese cymbidium seed germination, it is characterized in that, the method comprises the following steps:
(1) seed is selected: the Chinese cymbidium capsule choosing about 210d kind age;
(2) the surface of the seed process: Chinese cymbidium capsule after surface cleaning, is placed in superclean bench, after anhydrous filter paper blots surface moisture after plucking; After washing aseptic after the alcohol-pickled 5min of 75% 3-5 time, then after the mercury chloride surface sterilizing 10min using 0.1%, after aseptic washing 5-8 time;
(3) soak seed: capsule is cut open in the tea-bag sealed that Chinese cymbidium seed threading sterilization treatment is crossed; Then 10%H is adopted
2o
2aseptic washing 3 times after process 10min, 6-BA soaks 15min;
(4) sprout cultivation: the Chinese cymbidium seed after process is made the aqueous solution and sowed uniformly on germination medium, continues under being transposed to illumination after dark treatment 15d, cultivation temperature 25 DEG C, cultivate light application time 12h/d; Described germination medium is 1/2MS+0.05mg/LNAA+200mg/L Coconut Juice+20g/L sucrose+4g/L agar; The pH of described germination medium is 5.4-5.8.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510099892.XA CN104663070B (en) | 2015-03-06 | 2015-03-06 | A kind of method promoting Chinese cymbidium seed germination |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510099892.XA CN104663070B (en) | 2015-03-06 | 2015-03-06 | A kind of method promoting Chinese cymbidium seed germination |
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| Publication Number | Publication Date |
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| CN104663070A CN104663070A (en) | 2015-06-03 |
| CN104663070B true CN104663070B (en) | 2016-03-30 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799336A (en) * | 2005-12-16 | 2006-07-12 | 江苏阳光生态农林开发股份有限公司 | Method for cultivating cymbidium goeringii seed by tissue culture |
| CN101124892A (en) * | 2007-09-25 | 2008-02-20 | 云南省农业科学院高山经济植物研究所 | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method |
| CN101983554A (en) * | 2010-03-19 | 2011-03-09 | 西南林学院 | Reproduction method of Chinese cymbidium seedlings |
-
2015
- 2015-03-06 CN CN201510099892.XA patent/CN104663070B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799336A (en) * | 2005-12-16 | 2006-07-12 | 江苏阳光生态农林开发股份有限公司 | Method for cultivating cymbidium goeringii seed by tissue culture |
| CN101124892A (en) * | 2007-09-25 | 2008-02-20 | 云南省农业科学院高山经济植物研究所 | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method |
| CN101983554A (en) * | 2010-03-19 | 2011-03-09 | 西南林学院 | Reproduction method of Chinese cymbidium seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 提高墨兰繁殖速度的研究;朱根发等;《中国科协第二届青年学术年会，园艺学论文集》;19951231;第595-598页 * |
| 野生惠兰无菌播种及繁育技术研究;卜朝阳等;《种子》;20110930;第30卷(第9期);第18-21页 * |
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