CN104604390B - A kind of method of podocarpus nagi seed sprouting and rooting - Google Patents
A kind of method of podocarpus nagi seed sprouting and rooting Download PDFInfo
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- CN104604390B CN104604390B CN201510084138.9A CN201510084138A CN104604390B CN 104604390 B CN104604390 B CN 104604390B CN 201510084138 A CN201510084138 A CN 201510084138A CN 104604390 B CN104604390 B CN 104604390B
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Abstract
The present invention relates to a kind of methods of podocarpus nagi seed sprouting and rooting, the podocarpus nagi seed of fresh mature will be removed exosper, are put into 4oIt is refrigerated in C wind cooling refrigerator, taking-up when hand takes seed to have kernel to shake feeling is successively cleaned with detergent solution, sterile water；Successively alcohol, Hgcl on superclean bench again2Water-soluble liquid disinfectant is simultaneously net with aseptic water washing, the suck dry moisture on aseptic filter paper；Then shell rupture will be planted with pliers or be crushed, take out decladding seed with tweezers, be inoculated into culture medium and cultivate；The culture medium is 1/2MS culture medium, is added with sucrose, agar powder, PH5.5-5.8；Condition of culture: 25 ± 2 DEG C of temperature, is then used the illumination of 1500-2700Lux white light 12 hours first dark culture 7 days daily；To 50-55 days, blade 7-8 piece, highly reach 8-12cm seedling when, open bottle cap hardening 3-6 days, edulcoration root culture medium, implantation Light media culture bag in.
Description
Technical field
The invention belongs to botanical seedling culturing technical fields, and in particular to the method for podocarpus nagi seed sprouting and rooting.
Background technique
Podocarpus nagi seedling, treelet or potted landscape are verdant elegant because its leaf is like bamboo, while being that a kind of indoor sight leaf or air are net again
It is turned into object, by everybody popular welcome, sales volume increases severely in recent years；Podocarpus nagi seedling slow growth, from winter collect seed go exosper,
Refrigeration to the second spring is sowed, until growing 7-8 piece leaf needs 15 months or so；Not only slow growth, and by season shadow
It rings greatly, efficiency is lower；And in the production of the basin skill of podocarpus nagi, a large amount of seedlings are required throughout the year, therefore routinely seeding and seedling raising
Mode can not meet the needs of basin skill construction production production at all；Therefore it is badly in need of establishing in production a kind of utilizing the quick of seed
Raising plantlet, and the technology that seedling size, time are controllable are subject to seasonal restrictions, seedling slow growth and difficulty with solving seeding and seedling raising
The problem of control.
Summary of the invention
The object of the present invention is to provide a kind of methods of podocarpus nagi seed sprouting and rooting, using tissue culture technique not by season
Raw seedling leaf 7-8 piece can be improved by 95% or more, two month in section limitation, percentage of seedgermination, planting percent, highly reaches 8-12cm；It can be with
When basin skill constructs, throughout the year to the needs of a large amount of podocarpus nagi seedlings；And not by time restriction.
The technical scheme is that a kind of method of podocarpus nagi seed sprouting and rooting, includes the following steps:
A, the podocarpus nagi seed of fresh mature is removed into exosper, is put into 4oIt is refrigerated in C wind cooling refrigerator, takes seed to feel have to hand
When kernel shaking sound, seed is taken out；
B, it will be put into tap water and be rinsed only by the seed of A processing, and sufficiently be rinsed only with sterile water；
C, it will be placed on superclean bench by the seed of B processing, seed be placed in 75% alcohol and impregnates 20-30s
Afterwards, then with 0.1% Hgcl2Then aqueous solution soaking 5-13min is used aseptic water washing 5 times, is placed on aseptic filter paper and blots water
Point；
D, shell rupture or broken will will be planted by the processed seed pliers of C, be taken out decladding seed with tweezers, be inoculated into
It is cultivated in culture medium, which is 1/2MS culture medium, then adds sucrose 18-21g/L, agar powder 5.3-5.7g/L, adjusts PH
To 5.5-5.8；Condition of culture be at 25 ± 2 DEG C of temperature, first dark culture 7 days, then daily with 1500-2700Lux white light light
According to 12 hours；
E, it will pass through above-mentioned culture 50-55 days, and blade 7-8 piece can be obtained, highly up to 8-12cm seedling, open bottle cap hardening
3-6 days, edulcoration root culture medium was implanted into Light media culture bag.
Simple using the above method, convenient, easy to operate compared with current seeding and seedling raising, emergence rate is high, after 15 days, sees
Observe percentage of seedgermination, planting percent is increased to 95% or more, two months raw small seedling leaf 7-8 pieces, height 8-12cm；Than existing
The method of seeding and seedling raising compare, the time 10 months can be shortened, provide a newest technology for quick apply in production
System.This method is not subject to seasonal restrictions using tissue culture technique, and not by time restriction, seedling size is controllable, can be with 1 year four
To a large amount of needs of seedling when meeting podocarpus nagi basin skill construction season.
The method of the present invention is described further below with reference to embodiment.
Embodiment 1
The podocarpus nagi seed of fresh mature is removed into exosper, is put into 4oIt is refrigerated one month or so in C wind cooling refrigerator, takes kind to hand
When son has kernel to shake feeling, podocarpus nagi seed is put into tap water and is rinsed only, and sufficiently rinsed only with sterile water；In ultra-clean work
Make on platform, seed is placed on after impregnating 30s in 75% alcohol, then with 0.1% Hgcl2Aqueous solution soaking 5min, then uses nothing
Bacterium water rinses 5 times, is placed in suck dry moisture on aseptic filter paper；Shell rupture will be planted with pliers or will be crushed, and take out decladding seed with tweezers,
It is inoculated into 1/2MS culture medium and cultivates, addition sucrose 20g/L, agar powder 5.5g/L, active carbon 1.5g/L in culture medium, then plus
Enter 6-BA0.5mg/L, NAA0.3mg/L and KT0.5mg/L, adjusts PH to 5.7.Condition of culture is at 25 ± 2 DEG C of temperature, first
Dark culture 7 days, then 1500Lux white light illumination 12 hours daily.
After 15 days, observe that percentage of seedgermination, planting percent are increased to 95% or more；50 days or so by above-mentioned culture are i.e.
The seedling of blade 7-8 piece, height 8-12cm can be obtained, is opened bottle cap hardening 3-6 days, edulcoration root culture medium, implantation Light media training
It supports in bag.
Embodiment 2
The podocarpus nagi seed of fresh mature is removed into exosper, is put into 4oIt is refrigerated one month or so in C wind cooling refrigerator, takes kind to hand
When son has kernel to shake feeling, podocarpus nagi seed is put into tap water and is rinsed only, and sufficiently rinsed only with sterile water；In ultra-clean work
Make on platform, seed is placed on after impregnating 20s in 75% alcohol, then with 0.1% Hgcl2Aqueous solution soaking 13min, then uses nothing
Bacterium water rinses 5 times, is placed in suck dry moisture on aseptic filter paper；Shell rupture will be planted with pliers or will be crushed, and take out decladding seed with tweezers,
It is inoculated into 1/2MS culture medium and cultivates, addition sucrose 20g/L, agar powder 5.5g/L, active carbon 1.5g/L in culture medium, then plus
Enter 6-BA0.7mg/L, NAA0.5mg/L and KT0.7mg/L, adjusts PH to 5.7.Condition of culture is at 25 ± 2 DEG C of temperature, first
Dark culture 7 days, then 2700Lux white light illumination 12 hours daily.
After 15 days, observe that percentage of seedgermination, planting percent are increased to 95% or more；By above-mentioned culture 50 days, leaf can be obtained
The seedling of piece 7-8 piece, height 8-12cm is opened bottle cap hardening 3-6 days, edulcoration root culture medium, is implanted into Light media culture bag
?.
Claims (1)
1. a kind of method of podocarpus nagi seed sprouting and rooting, feature includes five steps of following A, B, C, D, E:
A, the podocarpus nagi seed of fresh mature is removed into exosper, is put into 4oIt is refrigerated in C wind cooling refrigerator, takes seed to have kernel shaking to hand
When feeling, seed is taken out；
B, it will be put into tap water and be rinsed only by the seed of A processing, and sufficiently be cleaned with sterile water；
C, it will be placed on superclean bench, seed be placed on after impregnating 20-30s in 75% alcohol by the seed of B processing, then
With 0.1% HgCl2Then aqueous solution soaking 5-13min uses aseptic water washing 5 times, is placed in suck dry moisture on aseptic filter paper；
D, shell rupture or broken will will be planted by the processed seed pliers of C, be taken out decladding seed with tweezers, be inoculated into culture
Cultivated in base, which is 1/2MS culture medium, then adds sucrose 18-21g/L, agar powder 5.3-5.7g/L, adjust pH to
5.5-5.8；Condition of culture is at 25 ± 2 DEG C of temperature, first dark culture 7 days, then 1500-2700Lux white light illumination 12 daily
Hour；
E, by 50-55 days Jing Guo above-mentioned culture, blade 7-8 piece, highly reach 8-12cm seedling when, open bottle cap hardening 3-6 days,
Edulcoration root culture medium is implanted into Light media culture bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510084138.9A CN104604390B (en) | 2015-02-16 | 2015-02-16 | A kind of method of podocarpus nagi seed sprouting and rooting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510084138.9A CN104604390B (en) | 2015-02-16 | 2015-02-16 | A kind of method of podocarpus nagi seed sprouting and rooting |
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| CN104604390A CN104604390A (en) | 2015-05-13 |
| CN104604390B true CN104604390B (en) | 2019-02-19 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969310A (en) * | 2017-12-19 | 2018-05-01 | 陈江海 | A kind of podocarpus nagi and red skin banyan Mixed forestation method |
| CN113303155A (en) * | 2021-05-03 | 2021-08-27 | 福建农林大学 | Method for dibbling and culturing young container seedlings by using bamboo and cypress seeds |
| CN113303123A (en) * | 2021-05-03 | 2021-08-27 | 福建农林大学 | LED auxiliary light cultivation method for promoting growth of bamboo cypress seedlings |
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2015
- 2015-02-16 CN CN201510084138.9A patent/CN104604390B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 竹柏的育苗技术;陈语亮;《林业科技》;20080630;第36卷(第2期) |
| 竹柏苗木繁育技术;彭自移等;《湖南林业科技》;20091231;第36卷(第6期);第43－44页 |
| 长叶竹柏的播种育苗;吴小英等;《林业实用技术》;20081231(第7期);第25－26页 |
| 顽拗性竹柏种子的贮藏特性;计明月等;《植物分类与资源学报》;20150122;第37卷(第1期);第64－65页第1.2.1、1.2.2、1.2.4、1.2.7节 |
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