CN105850747A - Rapid propagation method for tissue of succulent sedum rubrotinctum and sedum rubrotinctum cultured with method - Google Patents
Rapid propagation method for tissue of succulent sedum rubrotinctum and sedum rubrotinctum cultured with method Download PDFInfo
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- CN105850747A CN105850747A CN201610317835.9A CN201610317835A CN105850747A CN 105850747 A CN105850747 A CN 105850747A CN 201610317835 A CN201610317835 A CN 201610317835A CN 105850747 A CN105850747 A CN 105850747A
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- rainbow
- jade
- sedum
- rubrotinctum
- culture medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Botany (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a rapid propagation method for tissue of succulent sedum rubrotinctum, and sedum rubrotinctum cultured with the method, and belongs to the technical field of plant tissue culture. The main technical scheme adopted by the invention is as follows: selection and disinfection of explant: taking a leaf or a stem of sedum rubrotinctum as the explant, using ethyl alcohol for soaking the expant whose surface is washed cleanly, and using aqueous solution of mercuric chloride for soaking to obtain sterile explant; inoculating an inducted cluster bud culture medium with the sterile explant, and culturing for 2-3 weeks to obtain a complete plant. According to the method, a seedling of sedum rubrotinctum is obtained by tissue culture of the leaf or the stem of sedum rubrotinctum, materials are obtained easily, the disinfection is convenient, the rate of survival is high, the production cycle is short, the propagation coefficient and the propagation speed of sedum rubrotinctum are improved, the strain advantages of sedum rubrotinctum can be kept better, a large amount of seedlings of sedum rubrotinctum can be rapidly propagated, and the production requirement is met.
Description
Technical field
The present invention relates to the method for quickly breeding of a kind of plant tissue, be specially a kind of succulent
The tissue method for quickly breeding of the jade of rainbow and the jade of the rainbow of cultivation thereof, belong to Plant Tissue Breeding skill
Art field.
Background technology
Rainbow beautiful (Sedum rubrotinctum) is the succulent of Crassulaceae Sedum, many
Year raw meat matter herbaceous plant, the speed of growth is than very fast, and spring and summer is primary growth season, summer
35 degree of high temperature need to shelter from heat or light, its plant height 10-20 centimetre, multi-branched, and fleshy leaf expands alternate,
Cylindrical shape is to avette, and long 2 centimetres, green, epidermis is bright, without white powder, sun-drenched
Under the conditions of transfer bronzing to, the yellowish redness of little Hua, form is lovely, is deeply liked by everybody, views and admires
It is worth height.The cultivation of the jade of rainbow is usually taken cuttage, i.e. takes off intact leaf from stem, puts
Cuttage again after putting 3 days, uses the method that leaf injects row breeding, although breeding coefficient is big, but becomes
Type is relatively slow, and growth period is long, there is presently no the correlation technique of the jade Fast-propagation about rainbow.
Summary of the invention
For the deficiencies in the prior art, the present invention provides the tissue of the jade that a kind of succulent is red fast
Speed propagation method and the jade of rainbow of cultivation thereof, the method has easily operation, cultivation cycle is short, become
The feature that motility rate is high.
Technical scheme is as follows: the tissue Fast-propagation side of the jade of a kind of succulent rainbow
Method, the method comprises the steps: selection and the sterilization of (1) outer implant: with the jade blade of rainbow
Or stem section is outer implant, the alcohol of outer implant 70vt%-75vt% clean for surface clean is soaked
The bubble 20-30 second, then soak 3-10 minute with the mercuric chloride solution of 0.1wt%, soak every time
All wash with sterilized water after finishing, obtain aseptic outer implant；
(2) induction of Multiple Buds: aseptic outer implant is inoculated in induced bundle and sprouts in culture medium, with
When the stem section of the jade of rainbow does outer implant, inoculate after first stem section is cut into the segment of about 1cm
In culture medium, 23-26 DEG C, intensity of illumination 800-1500lux, light application time be 24
After cultivating 3-7 days in the environment of hour/day, relative humidity 55%-65%, Mr. is known from experience in outer planting
Root, continues to cultivate after 3-5 days and obtains Multiple Buds, and induced bundle culture medium of sprouting is to cultivate with MS
Base is minimal medium, adds 0.6-1.5mg 6-benzyl gland fast in every liter of minimal medium
Purine, 0.6-1.5mg heteroauxin, 25-30g sucrose, 8.0-8.4g agar, 3-7g activity
Charcoal, NaOH regulation pH value is 5.80-6.0, is preferably: 1.0mg 6-benzyladenine,
1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, NaOH regulates pH value
It is 5.82；
(3) subculture: the young shoot of Multiple Buds step (2) cultivation obtained cuts and is transferred to subculture training
Support in base, cultivate and 2-3 week i.e. can get whole plant；Subculture medium is with MS culture medium
For minimal medium, add in every liter of minimal medium 1.0mg 6-benzyladenine,
1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, pH value is 5.82.
(4) transplant: on its root of whole plant that will obtain through squamous subculture, the culture medium of residual rinses
Totally, In Shade air-dry after transplant to sand soil, shade, temperature be 18-26 DEG C,
Relative humidity is to grow under 55%-65%.
The present invention is claimed the jade cultivating the succulent rainbow obtained according to the method described above simultaneously.
Beneficial effects of the present invention is as follows: the present invention is by carrying out group to the jade blade of rainbow or stem section
Knitting cultivation and obtain the jade seedling of rainbow, the method material is easy to get, and sterilization is convenient, and survival rate is high,
With short production cycle, improve breeding coefficient and the reproduction speed of the jade of rainbow, and can preferably keep
The strain advantage of the jade of rainbow, energy Fast-propagation goes out the jade seedling of a large amount of excellent rainbow being suitable for and transplanting,
Meet Production requirement；Induced bundle in present invention culture medium of sprouting with the addition of the work of suitable concentration
Property charcoal absorption harmful substance not only externally the brown stain of implant have certain inhibitory action, and then improve
Improve the breeding coefficient of the jade of rainbow, reproduction speed and survival rate of plant, improve grower's
Economic benefit.
Accompanying drawing explanation
Fig. 1 is the photo that the blade that embodiment 1 obtains is taken root；
Fig. 2 is the photo of the Multiple Buds that embodiment 2 obtains；
Fig. 3 is the whole plant that embodiment 1 is transplanted in soil；
Fig. 4 is the photo of comparative example blade；
Fig. 5 is the photo of comparative example blade generation brown stain.
Detailed description of the invention
The present invention is described in more detail with embodiment below in conjunction with the accompanying drawings, but the guarantor of the present invention
The scope of protecting is not limited only to these embodiments, and if no special instructions, the present invention is raw materials used the most commercially available
Can obtain.
Embodiment 1
In the present embodiment: induced bundle culture medium of sprouting is with MS culture medium as minimal medium,
Every liter of minimal medium adds 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g
Sucrose, 8.0g agar, 5g activated carbon is formulated, then is 5.82 by NaOH regulation pH value；
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium
Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar,
5g activated carbon, then be 5.82 by NaOH regulation pH value.
(1) selection of outer implant and sterilization
Choose the jade plant of healthy rainbow, wash plant surface earth and rinse 15 with flowing water again
Minute.First with the jade plant of the alcohol-pickled rainbow that volume fraction is 75% on superclean bench
30 seconds, then with sterilized water washing 3 times, each 2 minutes；Take the blade conduct of the jade of rainbow
Outer implant, proceeds to outer implant soak 3 points in the mercuric chloride solution that mass fraction is 0.1%
Clock, then washs 6 times with sterilized water, each 2 minutes, obtains aseptic outer implant；
(2) induction of Multiple Buds
The induced bundle that aseptic outer implant is inoculated in tissue culture bottle is sprouted in culture medium, every bottle graft
Kinds 4,25 DEG C, intensity of illumination 1200lux, light application time be 24 hours/day, phase
Outer implant after cultivating 6 days in the environment of humidity 55% is taken root, germinates after continuing to cultivate 3 days
Obtain Multiple Buds；Through above-mentioned 9 days Fiber differentiation, the jade blade induced synthesis of rainbow was grown thickly
Bud；
(3) subculture: Multiple Buds is accessed and continues to cultivate obtain for 3 weeks taking root complete in subculture medium
Whole plant；
(4) transplant: the culture medium of residual on band whole plant root is rinsed well, In Shade
Air-dry 1 day, transplant in sand soil, shade, temperature be 22 DEG C, relative humidity be 55%
Lower growth.
Embodiment 2
In the present embodiment: induced bundle culture medium of sprouting is with MS culture medium as minimal medium,
Every liter of minimal medium adds 0.6mg 6-benzyladenine, 0.6mg heteroauxin, 25g
Sucrose, 8.0g agar, 3g activated carbon is formulated, then is 5.80 by NaOH regulation pH value；
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium
Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar,
5g activated carbon, then be 5.82 by NaOH regulation pH value.
(1) selection of outer implant and sterilization
Choose the jade plant of healthy rainbow, wash plant surface earth and rinse 15 with flowing water again
Minute.First with the jade plant of the alcohol-pickled rainbow that volume fraction is 70% on superclean bench
30 seconds, then with sterilized water washing 3 times, each 2 minutes；Take the stem section conduct of the jade of rainbow
Outer implant, proceeds to outer implant soak 3 points in the mercuric chloride solution that mass fraction is 0.1%
Clock, then washs 6 times with sterilized water, each 2 minutes, obtains aseptic outer implant；
(2) induction of Multiple Buds
Inoculate in culture medium after first stem section is cut into the segment of about 1cm, every bottle graft
Kinds 3 sections, 26 DEG C, intensity of illumination 1500lux, light application time be 24 hours/day, phase
Outer implant after cultivating 6 days in the environment of humidity 55% is taken root, germinates after continuing to cultivate 5 days
Obtain Multiple Buds；Through above-mentioned 11 days Fiber differentiation, the jade blade induced synthesis clump of rainbow
Sprout, see Fig. 2；
(3) subculture: Multiple Buds is accessed and continues to cultivate obtain for 2 weeks taking root complete in subculture medium
Whole plant；
(4) transplant: the culture medium of residual on band whole plant root is rinsed well, In Shade
Air-dry 1 day, transplant in sand soil, shade, temperature be 22 DEG C, relative humidity be 55%
Lower growth.
Embodiment 3
In the present embodiment: induced bundle culture medium of sprouting is with MS culture medium as minimal medium,
Every liter of minimal medium adds 1.5mg 6-benzyladenine, 1.5mg heteroauxin, 30g
Sucrose, 8.4g agar, 7g activated carbon is formulated, then is 6.0 by NaOH regulation pH value；
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium
Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar,
5g activated carbon, then be 5.82 by NaOH regulation pH value.
(1) selection of outer implant and sterilization
Choose the jade plant of healthy rainbow, wash plant surface earth and rinse 15 with flowing water again
Minute.First with the jade plant of the alcohol-pickled rainbow that volume fraction is 70% on superclean bench
30 seconds, then with sterilized water washing 3 times, each 2 minutes；Take the blade conduct of the jade of rainbow
Outer implant, proceeds to soak in the mercuric chloride solution that mass fraction is 0.1% 10 by outer implant
Minute, then wash 6 times with sterilized water, each 2 minutes, obtain aseptic outer implant；
(2) induction of Multiple Buds
The induced bundle that aseptic outer implant is inoculated in tissue culture bottle is sprouted in culture medium, every bottle graft
Kinds 4,24 DEG C, intensity of illumination 1000lux, light application time be 24 hours/day, phase
Outer implant after cultivating 6 days in the environment of humidity 65% is taken root, germinates after continuing to cultivate 5 days
Obtain Multiple Buds；Through above-mentioned 11 days Fiber differentiation, the jade blade induced synthesis clump of rainbow
Sprout；
(3) subculture: Multiple Buds is accessed and continues to cultivate obtain for 2 weeks taking root complete in subculture medium
Whole plant；
(4) transplant: the culture medium of residual on band whole plant root is rinsed well, In Shade
Air-dry 1 day, transplant in sand soil, shade, temperature be 22 DEG C, relative humidity be 65%
Lower growth.
Comparative example
In comparative example: induced bundle culture medium of sprouting is with MS culture medium as minimal medium, often
Rise and minimal medium adds 1.5mg 6-benzyladenine, 1.5mg heteroauxin, 30g
Sucrose, 8.4g agar are formulated, then are 6.0 by NaOH regulation pH value；
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium
Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar,
Regulating pH value with NaOH again is 5.82.
Comparative example is outer implant with the blade of the jade of rainbow, and cultural method, condition are complete with embodiment 1
Exactly the same.In the Induction Process of Multiple Buds, do not take root when cultivating to 6 days, the most do not send out
Bud, cultivates to partial blade generation brown stain in 10 days, sees Fig. 4-5.And use the present invention to add
The culture medium of activated carbon, grows whole plant through induction and the squamous subculture of Multiple Buds, sees figure
1、3.In sum, the induced bundle in the present invention sprouts culture medium and subculture medium with the addition of
The charcoal absorption harmful substance of suitable concentration, the brown stain of external implant has certain inhibitory action,
And then improve the breeding coefficient of the jade of rainbow, reproduction speed and survival rate of plant, improve
The economic benefit of grower.
Above the specific embodiment of the invention and application effect are explained in detail, but this
Bright it is not limited to above-mentioned embodiment, in the ken that those skilled in the art are possessed,
Can also various changes can be made without departing from the inventive concept of the premise.
Claims (7)
1. the tissue method for quickly breeding of the jade of a succulent rainbow, it is characterised in that the method bag
Include following steps:
(1) selection of outer implant and sterilization: by the jade 70vt%-75vt% of rainbow clean for surface clean
The alcohol-pickled 20-30 second, the jade blade or the stem section that take rainbow are outer implant, outer implant are used
The mercuric chloride solution of 0.1wt% soaks 3-10 minute, all uses sterilized water after having soaked every time
Washing, obtains aseptic outer implant；
(2) induction of Multiple Buds: aseptic outer implant is inoculated in induced bundle and sprouts in culture medium,
23-26 DEG C, intensity of illumination 800-1500lux, light application time be 24 hours/day, the wettest
Multiple Buds is obtained after cultivating 6-12 days in the environment of degree 55%-65%；Induction adventitious shoots culture
Base is with MS culture medium as minimal medium, adds in every liter of minimal medium
0.6-1.5mg 6-benzyladenine, 0.6-1.5mg heteroauxin, 25-30g sucrose,
8.0-8.4g agar, 3-7g activated carbon, pH value is 5.80-6.0；
(3) subculture, transplanting.
2. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, its feature
Being, described induced bundle culture medium of sprouting is with MS culture medium as minimal medium, to often
Rise and minimal medium adds 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g sugarcane
Sugar, 8.0g agar, 5g activated carbon, pH value is 5.82.
3. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, its feature
Being, described step (2) is inoculated in after the stem section of the jade of rainbow is cut into the segment of 1 centimetre
Induced bundle is sprouted in culture medium.
4. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, it is special
Levying and be, described subculture is: cut by the young shoot that step (2) cultivates the Multiple Buds obtained
It is transferred in subculture medium, cultivates and 2-3 week i.e. can get whole plant.
5. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 4, its feature
Being, described subculture medium is with MS culture medium as minimal medium, basic to every liter
In culture medium add 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g sucrose,
8.0g agar, 5g activated carbon, pH value is 5.82.
6. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, its feature
Being, described transplanting is: the training of residual on its root of whole plant that will obtain through squamous subculture
Foster base is rinsed well, In Shade air-dry after transplant to sand soil, shade, in temperature
For 18-26 DEG C, relative humidity be to grow under 55%-65%.
7. one kind obtains many according to any one described tissue method for quickly breeding of claim 1-6
The jade of meat plant rainbow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610317835.9A CN105850747B (en) | 2016-05-13 | 2016-05-13 | A kind of jade of the tissue rapid propagation method of the jade of succulent rainbow and its rainbow of culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610317835.9A CN105850747B (en) | 2016-05-13 | 2016-05-13 | A kind of jade of the tissue rapid propagation method of the jade of succulent rainbow and its rainbow of culture |
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| CN105850747A true CN105850747A (en) | 2016-08-17 |
| CN105850747B CN105850747B (en) | 2018-03-16 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577294A (en) * | 2016-12-24 | 2017-04-26 | 河南中医药大学 | In-vitro rapid propagation method for haworthia cooperi v. truncata |
| CN107182487A (en) * | 2017-05-31 | 2017-09-22 | 柳州市盛顺种植场 | A kind of beautiful butterfly breeding method |
| CN108719065A (en) * | 2018-05-21 | 2018-11-02 | 句容市茂润苗木有限公司 | A kind of succulent rapid propagation method |
| CN111066654A (en) * | 2019-12-11 | 2020-04-28 | 云南爱花多肉花卉有限公司 | Tissue culture rapid propagation method of succulent plants |
| CN112997881A (en) * | 2021-02-02 | 2021-06-22 | 中信建设有限责任公司 | Sedum aizoon explant sterilization method, callus induction culture medium and induction method |
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| CN101444185A (en) * | 2006-12-15 | 2009-06-03 | 浙江大学 | Method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit |
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| CN101444185A (en) * | 2006-12-15 | 2009-06-03 | 浙江大学 | Method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577294A (en) * | 2016-12-24 | 2017-04-26 | 河南中医药大学 | In-vitro rapid propagation method for haworthia cooperi v. truncata |
| CN107182487A (en) * | 2017-05-31 | 2017-09-22 | 柳州市盛顺种植场 | A kind of beautiful butterfly breeding method |
| CN108719065A (en) * | 2018-05-21 | 2018-11-02 | 句容市茂润苗木有限公司 | A kind of succulent rapid propagation method |
| CN111066654A (en) * | 2019-12-11 | 2020-04-28 | 云南爱花多肉花卉有限公司 | Tissue culture rapid propagation method of succulent plants |
| CN112997881A (en) * | 2021-02-02 | 2021-06-22 | 中信建设有限责任公司 | Sedum aizoon explant sterilization method, callus induction culture medium and induction method |
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