CN105850747A - Rapid propagation method for tissue of succulent sedum rubrotinctum and sedum rubrotinctum cultured with method - Google Patents

Rapid propagation method for tissue of succulent sedum rubrotinctum and sedum rubrotinctum cultured with method Download PDF

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Publication number
CN105850747A
CN105850747A CN201610317835.9A CN201610317835A CN105850747A CN 105850747 A CN105850747 A CN 105850747A CN 201610317835 A CN201610317835 A CN 201610317835A CN 105850747 A CN105850747 A CN 105850747A
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China
Prior art keywords
rainbow
jade
sedum
rubrotinctum
tissue
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CN201610317835.9A
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CN105850747B (en
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金华
程文琼
郭鹏
李琳琳
邹吉祥
张颖
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Dalian Minzu University
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Dalian Nationalities University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy

Abstract

The invention relates to a rapid propagation method for tissue of succulent sedum rubrotinctum, and sedum rubrotinctum cultured with the method, and belongs to the technical field of plant tissue culture. The main technical scheme adopted by the invention is as follows: selection and disinfection of explant: taking a leaf or a stem of sedum rubrotinctum as the explant, using ethyl alcohol for soaking the expant whose surface is washed cleanly, and using aqueous solution of mercuric chloride for soaking to obtain sterile explant; inoculating an inducted cluster bud culture medium with the sterile explant, and culturing for 2-3 weeks to obtain a complete plant. According to the method, a seedling of sedum rubrotinctum is obtained by tissue culture of the leaf or the stem of sedum rubrotinctum, materials are obtained easily, the disinfection is convenient, the rate of survival is high, the production cycle is short, the propagation coefficient and the propagation speed of sedum rubrotinctum are improved, the strain advantages of sedum rubrotinctum can be kept better, a large amount of seedlings of sedum rubrotinctum can be rapidly propagated, and the production requirement is met.

Description

The tissue method for quickly breeding of the jade of a kind of succulent rainbow and the jade of the rainbow of cultivation thereof
Technical field
The present invention relates to the method for quickly breeding of a kind of plant tissue, be specially a kind of succulent The tissue method for quickly breeding of the jade of rainbow and the jade of the rainbow of cultivation thereof, belong to Plant Tissue Breeding skill Art field.
Background technology
Rainbow beautiful (Sedum rubrotinctum) is the succulent of Crassulaceae Sedum, many Year raw meat matter herbaceous plant, the speed of growth is than very fast, and spring and summer is primary growth season, summer 35 degree of high temperature need to shelter from heat or light, its plant height 10-20 centimetre, multi-branched, and fleshy leaf expands alternate, Cylindrical shape is to avette, and long 2 centimetres, green, epidermis is bright, without white powder, sun-drenched Under the conditions of transfer bronzing to, the yellowish redness of little Hua, form is lovely, is deeply liked by everybody, views and admires It is worth height.The cultivation of the jade of rainbow is usually taken cuttage, i.e. takes off intact leaf from stem, puts Cuttage again after putting 3 days, uses the method that leaf injects row breeding, although breeding coefficient is big, but becomes Type is relatively slow, and growth period is long, there is presently no the correlation technique of the jade Fast-propagation about rainbow.
Summary of the invention
For the deficiencies in the prior art, the present invention provides the tissue of the jade that a kind of succulent is red fast Speed propagation method and the jade of rainbow of cultivation thereof, the method has easily operation, cultivation cycle is short, become The feature that motility rate is high.
Technical scheme is as follows: the tissue Fast-propagation side of the jade of a kind of succulent rainbow Method, the method comprises the steps: selection and the sterilization of (1) outer implant: with the jade blade of rainbow Or stem section is outer implant, the alcohol of outer implant 70vt%-75vt% clean for surface clean is soaked The bubble 20-30 second, then soak 3-10 minute with the mercuric chloride solution of 0.1wt%, soak every time All wash with sterilized water after finishing, obtain aseptic outer implant;
(2) induction of Multiple Buds: aseptic outer implant is inoculated in induced bundle and sprouts in culture medium, with When the stem section of the jade of rainbow does outer implant, inoculate after first stem section is cut into the segment of about 1cm In culture medium, 23-26 DEG C, intensity of illumination 800-1500lux, light application time be 24 After cultivating 3-7 days in the environment of hour/day, relative humidity 55%-65%, Mr. is known from experience in outer planting Root, continues to cultivate after 3-5 days and obtains Multiple Buds, and induced bundle culture medium of sprouting is to cultivate with MS Base is minimal medium, adds 0.6-1.5mg 6-benzyl gland fast in every liter of minimal medium Purine, 0.6-1.5mg heteroauxin, 25-30g sucrose, 8.0-8.4g agar, 3-7g activity Charcoal, NaOH regulation pH value is 5.80-6.0, is preferably: 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, NaOH regulates pH value It is 5.82;
(3) subculture: the young shoot of Multiple Buds step (2) cultivation obtained cuts and is transferred to subculture training Support in base, cultivate and 2-3 week i.e. can get whole plant;Subculture medium is with MS culture medium For minimal medium, add in every liter of minimal medium 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, pH value is 5.82.
(4) transplant: on its root of whole plant that will obtain through squamous subculture, the culture medium of residual rinses Totally, In Shade air-dry after transplant to sand soil, shade, temperature be 18-26 DEG C, Relative humidity is to grow under 55%-65%.
The present invention is claimed the jade cultivating the succulent rainbow obtained according to the method described above simultaneously.
Beneficial effects of the present invention is as follows: the present invention is by carrying out group to the jade blade of rainbow or stem section Knitting cultivation and obtain the jade seedling of rainbow, the method material is easy to get, and sterilization is convenient, and survival rate is high, With short production cycle, improve breeding coefficient and the reproduction speed of the jade of rainbow, and can preferably keep The strain advantage of the jade of rainbow, energy Fast-propagation goes out the jade seedling of a large amount of excellent rainbow being suitable for and transplanting, Meet Production requirement;Induced bundle in present invention culture medium of sprouting with the addition of the work of suitable concentration Property charcoal absorption harmful substance not only externally the brown stain of implant have certain inhibitory action, and then improve Improve the breeding coefficient of the jade of rainbow, reproduction speed and survival rate of plant, improve grower's Economic benefit.
Accompanying drawing explanation
Fig. 1 is the photo that the blade that embodiment 1 obtains is taken root;
Fig. 2 is the photo of the Multiple Buds that embodiment 2 obtains;
Fig. 3 is the whole plant that embodiment 1 is transplanted in soil;
Fig. 4 is the photo of comparative example blade;
Fig. 5 is the photo of comparative example blade generation brown stain.
Detailed description of the invention
The present invention is described in more detail with embodiment below in conjunction with the accompanying drawings, but the guarantor of the present invention The scope of protecting is not limited only to these embodiments, and if no special instructions, the present invention is raw materials used the most commercially available Can obtain.
Embodiment 1
In the present embodiment: induced bundle culture medium of sprouting is with MS culture medium as minimal medium, Every liter of minimal medium adds 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g Sucrose, 8.0g agar, 5g activated carbon is formulated, then is 5.82 by NaOH regulation pH value;
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, then be 5.82 by NaOH regulation pH value.
(1) selection of outer implant and sterilization
Choose the jade plant of healthy rainbow, wash plant surface earth and rinse 15 with flowing water again Minute.First with the jade plant of the alcohol-pickled rainbow that volume fraction is 75% on superclean bench 30 seconds, then with sterilized water washing 3 times, each 2 minutes;Take the blade conduct of the jade of rainbow Outer implant, proceeds to outer implant soak 3 points in the mercuric chloride solution that mass fraction is 0.1% Clock, then washs 6 times with sterilized water, each 2 minutes, obtains aseptic outer implant;
(2) induction of Multiple Buds
The induced bundle that aseptic outer implant is inoculated in tissue culture bottle is sprouted in culture medium, every bottle graft Kinds 4,25 DEG C, intensity of illumination 1200lux, light application time be 24 hours/day, phase Outer implant after cultivating 6 days in the environment of humidity 55% is taken root, germinates after continuing to cultivate 3 days Obtain Multiple Buds;Through above-mentioned 9 days Fiber differentiation, the jade blade induced synthesis of rainbow was grown thickly Bud;
(3) subculture: Multiple Buds is accessed and continues to cultivate obtain for 3 weeks taking root complete in subculture medium Whole plant;
(4) transplant: the culture medium of residual on band whole plant root is rinsed well, In Shade Air-dry 1 day, transplant in sand soil, shade, temperature be 22 DEG C, relative humidity be 55% Lower growth.
Embodiment 2
In the present embodiment: induced bundle culture medium of sprouting is with MS culture medium as minimal medium, Every liter of minimal medium adds 0.6mg 6-benzyladenine, 0.6mg heteroauxin, 25g Sucrose, 8.0g agar, 3g activated carbon is formulated, then is 5.80 by NaOH regulation pH value;
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, then be 5.82 by NaOH regulation pH value.
(1) selection of outer implant and sterilization
Choose the jade plant of healthy rainbow, wash plant surface earth and rinse 15 with flowing water again Minute.First with the jade plant of the alcohol-pickled rainbow that volume fraction is 70% on superclean bench 30 seconds, then with sterilized water washing 3 times, each 2 minutes;Take the stem section conduct of the jade of rainbow Outer implant, proceeds to outer implant soak 3 points in the mercuric chloride solution that mass fraction is 0.1% Clock, then washs 6 times with sterilized water, each 2 minutes, obtains aseptic outer implant;
(2) induction of Multiple Buds
Inoculate in culture medium after first stem section is cut into the segment of about 1cm, every bottle graft Kinds 3 sections, 26 DEG C, intensity of illumination 1500lux, light application time be 24 hours/day, phase Outer implant after cultivating 6 days in the environment of humidity 55% is taken root, germinates after continuing to cultivate 5 days Obtain Multiple Buds;Through above-mentioned 11 days Fiber differentiation, the jade blade induced synthesis clump of rainbow Sprout, see Fig. 2;
(3) subculture: Multiple Buds is accessed and continues to cultivate obtain for 2 weeks taking root complete in subculture medium Whole plant;
(4) transplant: the culture medium of residual on band whole plant root is rinsed well, In Shade Air-dry 1 day, transplant in sand soil, shade, temperature be 22 DEG C, relative humidity be 55% Lower growth.
Embodiment 3
In the present embodiment: induced bundle culture medium of sprouting is with MS culture medium as minimal medium, Every liter of minimal medium adds 1.5mg 6-benzyladenine, 1.5mg heteroauxin, 30g Sucrose, 8.4g agar, 7g activated carbon is formulated, then is 6.0 by NaOH regulation pH value;
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, then be 5.82 by NaOH regulation pH value.
(1) selection of outer implant and sterilization
Choose the jade plant of healthy rainbow, wash plant surface earth and rinse 15 with flowing water again Minute.First with the jade plant of the alcohol-pickled rainbow that volume fraction is 70% on superclean bench 30 seconds, then with sterilized water washing 3 times, each 2 minutes;Take the blade conduct of the jade of rainbow Outer implant, proceeds to soak in the mercuric chloride solution that mass fraction is 0.1% 10 by outer implant Minute, then wash 6 times with sterilized water, each 2 minutes, obtain aseptic outer implant;
(2) induction of Multiple Buds
The induced bundle that aseptic outer implant is inoculated in tissue culture bottle is sprouted in culture medium, every bottle graft Kinds 4,24 DEG C, intensity of illumination 1000lux, light application time be 24 hours/day, phase Outer implant after cultivating 6 days in the environment of humidity 65% is taken root, germinates after continuing to cultivate 5 days Obtain Multiple Buds;Through above-mentioned 11 days Fiber differentiation, the jade blade induced synthesis clump of rainbow Sprout;
(3) subculture: Multiple Buds is accessed and continues to cultivate obtain for 2 weeks taking root complete in subculture medium Whole plant;
(4) transplant: the culture medium of residual on band whole plant root is rinsed well, In Shade Air-dry 1 day, transplant in sand soil, shade, temperature be 22 DEG C, relative humidity be 65% Lower growth.
Comparative example
In comparative example: induced bundle culture medium of sprouting is with MS culture medium as minimal medium, often Rise and minimal medium adds 1.5mg 6-benzyladenine, 1.5mg heteroauxin, 30g Sucrose, 8.4g agar are formulated, then are 6.0 by NaOH regulation pH value;
Subculture medium is with MS culture medium as minimal medium, in every liter of minimal medium Add 1.0mg6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, Regulating pH value with NaOH again is 5.82.
Comparative example is outer implant with the blade of the jade of rainbow, and cultural method, condition are complete with embodiment 1 Exactly the same.In the Induction Process of Multiple Buds, do not take root when cultivating to 6 days, the most do not send out Bud, cultivates to partial blade generation brown stain in 10 days, sees Fig. 4-5.And use the present invention to add The culture medium of activated carbon, grows whole plant through induction and the squamous subculture of Multiple Buds, sees figure 1、3.In sum, the induced bundle in the present invention sprouts culture medium and subculture medium with the addition of The charcoal absorption harmful substance of suitable concentration, the brown stain of external implant has certain inhibitory action, And then improve the breeding coefficient of the jade of rainbow, reproduction speed and survival rate of plant, improve The economic benefit of grower.
Above the specific embodiment of the invention and application effect are explained in detail, but this Bright it is not limited to above-mentioned embodiment, in the ken that those skilled in the art are possessed, Can also various changes can be made without departing from the inventive concept of the premise.

Claims (7)

1. the tissue method for quickly breeding of the jade of a succulent rainbow, it is characterised in that the method bag Include following steps:
(1) selection of outer implant and sterilization: by the jade 70vt%-75vt% of rainbow clean for surface clean The alcohol-pickled 20-30 second, the jade blade or the stem section that take rainbow are outer implant, outer implant are used The mercuric chloride solution of 0.1wt% soaks 3-10 minute, all uses sterilized water after having soaked every time Washing, obtains aseptic outer implant;
(2) induction of Multiple Buds: aseptic outer implant is inoculated in induced bundle and sprouts in culture medium, 23-26 DEG C, intensity of illumination 800-1500lux, light application time be 24 hours/day, the wettest Multiple Buds is obtained after cultivating 6-12 days in the environment of degree 55%-65%;Induction adventitious shoots culture Base is with MS culture medium as minimal medium, adds in every liter of minimal medium 0.6-1.5mg 6-benzyladenine, 0.6-1.5mg heteroauxin, 25-30g sucrose, 8.0-8.4g agar, 3-7g activated carbon, pH value is 5.80-6.0;
(3) subculture, transplanting.
2. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, its feature Being, described induced bundle culture medium of sprouting is with MS culture medium as minimal medium, to often Rise and minimal medium adds 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g sugarcane Sugar, 8.0g agar, 5g activated carbon, pH value is 5.82.
3. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, its feature Being, described step (2) is inoculated in after the stem section of the jade of rainbow is cut into the segment of 1 centimetre Induced bundle is sprouted in culture medium.
4. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, it is special Levying and be, described subculture is: cut by the young shoot that step (2) cultivates the Multiple Buds obtained It is transferred in subculture medium, cultivates and 2-3 week i.e. can get whole plant.
5. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 4, its feature Being, described subculture medium is with MS culture medium as minimal medium, basic to every liter In culture medium add 1.0mg 6-benzyladenine, 1.0mg heteroauxin, 30g sucrose, 8.0g agar, 5g activated carbon, pH value is 5.82.
6. the tissue method for quickly breeding of the jade of succulent rainbow as claimed in claim 1, its feature Being, described transplanting is: the training of residual on its root of whole plant that will obtain through squamous subculture Foster base is rinsed well, In Shade air-dry after transplant to sand soil, shade, in temperature For 18-26 DEG C, relative humidity be to grow under 55%-65%.
7. one kind obtains many according to any one described tissue method for quickly breeding of claim 1-6 The jade of meat plant rainbow.
CN201610317835.9A 2016-05-13 2016-05-13 A kind of jade of the tissue rapid propagation method of the jade of succulent rainbow and its rainbow of culture Active CN105850747B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106577294A (en) * 2016-12-24 2017-04-26 河南中医药大学 In-vitro rapid propagation method for haworthia cooperi v. truncata
CN107182487A (en) * 2017-05-31 2017-09-22 柳州市盛顺种植场 A kind of beautiful butterfly breeding method
CN108719065A (en) * 2018-05-21 2018-11-02 句容市茂润苗木有限公司 A kind of succulent rapid propagation method

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106577294A (en) * 2016-12-24 2017-04-26 河南中医药大学 In-vitro rapid propagation method for haworthia cooperi v. truncata
CN107182487A (en) * 2017-05-31 2017-09-22 柳州市盛顺种植场 A kind of beautiful butterfly breeding method
CN108719065A (en) * 2018-05-21 2018-11-02 句容市茂润苗木有限公司 A kind of succulent rapid propagation method

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