CN104604390A - Method for rapidly obtaining seedlings from podocarpus nagi seeds - Google Patents
Method for rapidly obtaining seedlings from podocarpus nagi seeds Download PDFInfo
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- CN104604390A CN104604390A CN201510084138.9A CN201510084138A CN104604390A CN 104604390 A CN104604390 A CN 104604390A CN 201510084138 A CN201510084138 A CN 201510084138A CN 104604390 A CN104604390 A CN 104604390A
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Abstract
The invention relates to a method for rapidly obtaining seedlings from podocarpus nagi seeds. The method for rapidly obtaining the seedlings from the podocarpus nagi seeds comprises the following steps: removing testae of fresh and mature podocarpus nagi seeds, placing the obtained podocarpus nagi seeds into a 40 DEG C air-cooled refrigerator to be refrigerated, taking out the podocarpus nagi seeds when a worker feels that kernels shake on hands, sequentially washing the podocarpus nagi seeds with washing powder solution and sterile water; then sequentially disinfecting the podocarpus nagi seeds with ethyl alcohol and HgCl2 aqueous solution on an ultra-clean working platform, washing with sterile water, and sucking moisture on sterile filter papers until the podocarpus nagi seeds are dry; and then fracturing or crushing podocarpus nagi seed shells by adopting pliers, taking out shelled podocarpus nagi seeds by adopting tweezers, and inoculating the podocarpus nagi seeds into a culture medium to be cultured, wherein the culture medium is 1/2MS culture medium, cane sugar and agar powder are added, and pH is 5.5-5.8; and culture conditions comprise that dark culture is carried out for 7 days firstly at the temperature of 25+/-2 DEG C, then illumination is carried out for 12 hours every day by utilizing 1500-2700Lux white light, bottle caps are opened and seedlings are hardened for 3-6 days when plantlets which have 7-8 blades and 8-12cm high are obtained at the 50th-55th days, the culture medium at the roots are cleaned and the plantlets are implanted into light medium culture bags.
Description
Technical field
The invention belongs to botanical seedling culturing technical field, be specifically related to the method for podocarpus nagi seed sprouting and rooting.
Background technology
Podocarpus nagi seedling, treelet or potted landscape are because its leaf is like bamboo, verdant elegant, are again a kind of indoor sight leaf or air cleaning crop simultaneously, are subject to everybody popular welcome, and sales volume increases severely in recent years; Podocarpus nagi seedling poor growth, removes exosper from seed collecting in winter, refrigerates to sowing in the second spring, until grow 7-8 sheet leaf to need about 15 months; Not only poor growth, and large by seasonal effect, and efficiency is lower; And in the basin skill of podocarpus nagi makes, all need a large amount of seedling throughout the year, therefore Mode of sowing and seedling propagation cannot meet the needs that basin skill structure makes production at all routinely; Therefore produce upper urgent need and will set up a kind of sprouting and rooting technology utilizing seed, and the technology that seedling size, time are controlled, be subject to seasonal restrictions to solve seeding and seedling raising, seedling poor growth and problem rambunctious.
Summary of the invention
The object of this invention is to provide a kind of method of podocarpus nagi seed sprouting and rooting, it utilizes tissue culture technique not to be subject to seasonal restrictions, and percentage of seedgermination, planting percent can bring up to more than 95%, and two months raw seedling leaf 7-8 sheets, highly reach 8-12cm; Can basin skill structure time, throughout the year to the needs of a large amount of podocarpus nagi seedling; And not by time restriction.
Technical scheme of the present invention is: a kind of method of podocarpus nagi seed sprouting and rooting, comprises the steps:
A, the podocarpus nagi seed of fresh mature is removed exosper, be put into 4
orefrigerate in C wind cooling refrigerator, when hand-held seed feels have kind of benevolence to rock sound, take out seed;
B, the seed through A process put into running water and rinse clean, and fully rinse only with sterile water;
C, the seed through B process is placed on superclean bench, seed is placed on after soaking 20-30s in 75% alcohol, then uses the Hgcl of 0.1%
2aqueous solution soaking 5-13min, then uses aseptic water washing 5 times, is placed in suck dry moisture on aseptic filter paper;
D, to be broken by kind of a shell by the seed pliers through C process or broken, take out with tweezers the seed that shells, be inoculated in medium and cultivate, this medium is 1/2MS medium, then adds sucrose 18-21g/L, agar powder 5.3-5.7g/L, regulates PH to 5.5-5.8; Condition of culture be at temperature 25 ± 2 DEG C, first light culture 7 days, then every day 1500-2700Lux white light illumination 12 hours;
E, through above-mentioned cultivation 50-55 days, will can to obtain blade 7-8 sheet, highly reach 8-12cm seedling, open bottle cap hardening 3-6 days, edulcoration root medium, to implant in Light media culture bag.
Adopt said method simple, convenient, compared with current seeding and seedling raising, simple to operate, emergence rate is high, after 15 days, observes percentage of seedgermination, planting percent brings up to more than 95%, the little seedling leaf 7-8 sheet of life in two months, height 8-12cm; Compare than the method for existing seeding and seedling raising, 10 months time can be shortened, for the quick application on producing provides a up-to-date technical system.The method utilizes tissue culture technique not to be subject to seasonal restrictions, and not by time restriction, seedling size is controlled, can meet needs a large amount of to seedling when podocarpus nagi basin skill constructs throughout the year.
Below in conjunction with embodiment, the inventive method is described further.
embodiment 1
The podocarpus nagi seed of fresh mature is removed exosper, is put into 4
orefrigerate about one month in C wind cooling refrigerator, when hand-held seed has kind of benevolence to rock sensation, podocarpus nagi seed is put into running water and rinse clean, and fully rinse only with sterile water; On superclean bench, seed is placed on after soaking 30s in 75% alcohol, then uses the Hgcl of 0.1%
2aqueous solution soaking 5min, then uses aseptic water washing 5 times, is placed in suck dry moisture on aseptic filter paper; With pliers, kind of a shell is broken or fragmentation, take out with tweezers the seed that shells, be inoculated in 1/2MS medium and cultivate, in medium, add sucrose 20g/L, agar powder 5.5g/L, active carbon 1.5g/L, add 6-BA0.5mg/L, NAA0.3mg/L and KT0.5mg/L again, regulate PH to 5.7.Condition of culture be at temperature 25 ± 2 DEG C, first light culture 7 days, then 1500Lux white light illumination every day 12 hours.
After 15 days, observe percentage of seedgermination, planting percent brings up to more than 95%; Through about 50 days of above-mentioned cultivation blade 7-8 sheet, height 8-12cm seedling, open bottle cap hardening 3-6 days, edulcoration root medium, implant in Light media culture bag.
embodiment 2
The podocarpus nagi seed of fresh mature is removed exosper, is put into 4
orefrigerate about one month in C wind cooling refrigerator, when hand-held seed has kind of benevolence to rock sensation, podocarpus nagi seed is put into running water and rinse clean, and fully rinse only with sterile water; On superclean bench, seed is placed on after soaking 20s in 75% alcohol, then uses the Hgcl of 0.1%
2aqueous solution soaking 13min, then uses aseptic water washing 5 times, is placed in suck dry moisture on aseptic filter paper; With pliers, kind of a shell is broken or fragmentation, take out with tweezers the seed that shells, be inoculated in 1/2MS medium and cultivate, in medium, add sucrose 20g/L, agar powder 5.5g/L, active carbon 1.5g/L, add 6-BA0.7mg/L, NAA0.5mg/L and KT0.7mg/L again, regulate PH to 5.7.Condition of culture be at temperature 25 ± 2 DEG C, first light culture 7 days, then 2700Lux white light illumination every day 12 hours.
After 15 days, observe percentage of seedgermination, planting percent brings up to more than 95%; Through above-mentioned cultivation 50 days, blade 7-8 sheet, height 8-12cm seedling, open bottle cap hardening 3-6 days, edulcoration root medium, implant in Light media culture bag.
Claims (1)
1. a method for podocarpus nagi seed sprouting and rooting, its feature comprises following A, B, C, D, E five steps:
A, the podocarpus nagi seed of fresh mature is removed exosper, be put into 4
orefrigerate in C wind cooling refrigerator, until hand get seed have kind of benevolence to rock sensation time, take out seed;
B, the seed through A process put into running water and rinse clean, and fully clean with sterile water;
C, the seed through B process is placed on superclean bench, seed is placed on after soaking 20-30s in 75% alcohol, then uses the Hgcl of 0.1%
2aqueous solution soaking 5-13min, then uses aseptic water washing 5 times, is placed in suck dry moisture on aseptic filter paper;
D, to be broken by kind of a shell by the seed pliers through C process or broken, take out with tweezers the seed that shells, be inoculated in medium and cultivate, this medium is 1/2MS medium, then adds sucrose 18-21g/L, agar powder 5.3-5.7g/L, regulates PH to 5.5-5.8; Condition of culture be at temperature 25 ± 2 DEG C, first light culture 7 days, then 1500-2700Lux white light illumination every day 12 hours;
E, by through 50-55 days of above-mentioned cultivation, blade 7-8 sheet, highly reach 8-12cm seedling, open bottle cap hardening 3-6 days, edulcoration root medium, implant in Light media culture bag.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510084138.9A CN104604390B (en) | 2015-02-16 | 2015-02-16 | A kind of method of podocarpus nagi seed sprouting and rooting |
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| CN201510084138.9A CN104604390B (en) | 2015-02-16 | 2015-02-16 | A kind of method of podocarpus nagi seed sprouting and rooting |
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| CN104604390A true CN104604390A (en) | 2015-05-13 |
| CN104604390B CN104604390B (en) | 2019-02-19 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969310A (en) * | 2017-12-19 | 2018-05-01 | 陈江海 | A kind of podocarpus nagi and red skin banyan Mixed forestation method |
| CN113303123A (en) * | 2021-05-03 | 2021-08-27 | 福建农林大学 | LED auxiliary light cultivation method for promoting growth of bamboo cypress seedlings |
| CN113303155A (en) * | 2021-05-03 | 2021-08-27 | 福建农林大学 | Method for dibbling and culturing young container seedlings by using bamboo and cypress seeds |
-
2015
- 2015-02-16 CN CN201510084138.9A patent/CN104604390B/en not_active Expired - Fee Related
Non-Patent Citations (5)
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| 吴小英等: "长叶竹柏的播种育苗", 《林业实用技术》 * |
| 彭自移等: "竹柏苗木繁育技术", 《湖南林业科技》 * |
| 计明月等: "顽拗性竹柏种子的贮藏特性", 《植物分类与资源学报》 * |
| 谢云: "《园林苗木生产技术手册》", 31 January 2012 * |
| 陈语亮: "竹柏的育苗技术", 《林业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969310A (en) * | 2017-12-19 | 2018-05-01 | 陈江海 | A kind of podocarpus nagi and red skin banyan Mixed forestation method |
| CN113303123A (en) * | 2021-05-03 | 2021-08-27 | 福建农林大学 | LED auxiliary light cultivation method for promoting growth of bamboo cypress seedlings |
| CN113303155A (en) * | 2021-05-03 | 2021-08-27 | 福建农林大学 | Method for dibbling and culturing young container seedlings by using bamboo and cypress seeds |
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| CN104604390B (en) | 2019-02-19 |
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