CN107493916A - A kind of hardening off method of bamboo shoot orchid tissue culture seedling - Google Patents
A kind of hardening off method of bamboo shoot orchid tissue culture seedling Download PDFInfo
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- CN107493916A CN107493916A CN201710876297.1A CN201710876297A CN107493916A CN 107493916 A CN107493916 A CN 107493916A CN 201710876297 A CN201710876297 A CN 201710876297A CN 107493916 A CN107493916 A CN 107493916A
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- Prior art keywords
- hardening
- product
- bamboo shoot
- tissue culture
- culture seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Mechanical Engineering (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of hardening off method of bamboo shoot orchid tissue culture seedling, step includes：A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 2 ~ 3 days at room temperature, then placement 2 ~ 3 days of uncapping, obtain A product；B, A product are cleaned, soaked with carbendazim solution, dry surface moisture, obtain B product；C, wood sawdust, fertile soil are mixed as hardening matrix, after hardening matrix sterilization, adds rapeseed cake fertilizer, obtain C product；D, C product are layered on seedbed, B product is then transplanted to hardening 85 95 days on C product.The present invention can improve the survival rate of bamboo shoot orchid tissue culture seedling, up to more than 95%, can promote seedling development, accelerate the speed of growth, in 3 months can seedling, it is and simple to operate.
Description
Technical field
The present invention relates to plant hardening technical field, particularly a kind of hardening off method of bamboo shoot orchid tissue culture seedling.
Background technology
The also known as logical orchid of bamboo shoot orchid (Thunia alba (Lindl.) Rchb.f.), belongs to orchid family (Orchidaceae) bamboo shoot Cymbidium
(Thunia) give birth to or the herbaceous plant that grows nonparasitically upon another plant.Bamboo shoot orchid summer-flowering, basidixed raceme, spend big and beautiful, tool fragrance.Main point
Cloth, to south and Eastern Tibet, is born on sylvan life rock or crotch recess in the Yunnan Province of China west and south.It is blue to bamboo shoot in the market
Demand be mainly manifested in view and admire with medicinal two aspect, the flowers cultural and historical of China orchid is long, orchid have it is higher
Ornamental value, be called one of four gentleman in spending；Bamboo shoot orchid is also Ethnic crude drugs (seedling medicine) simply, with all herbal medicine, the property of medicine is light,
It is flat, there is the effect of promoting blood circulation, dissolving stasis, synthetism, myogenic.Current bamboo shoot orchid wild resource is exhausted, and existing resource can not meet currently
The market demand, artificial growth will be the important channel that solves its shortage of resources, and one of blue key technology of artificial growth bamboo shoot
It is seedling fostering and hardening.
Bamboo shoot orchid tissue-cultured seedling is to pass through the tissue cultures such as Multiplying culture, plant induction, culture of rootage using bamboo shoot orchid stem-tip tissue
The seedling that process is formed, seedling can be used as to carry out hardening.But conventionally carrying out hardening, (hardening matrix is soil, certainly
Right condition lower refining seedling), have that bamboo shoot orchid tissue culture seedling percent is low, and in the range of 50%-60%, the speed of growth is slow, it usually needs
The problem of crop field being just transplanted in 5-6 months.
The content of the invention
It is an object of the present invention to provide a kind of hardening off method of bamboo shoot orchid tissue culture seedling.The present invention can improve bamboo shoot orchid group
Train the survival rate of seedling, up to more than 95%, accelerate the speed of growth, in 3 months can seedling, it is and simple to operate.
Technical scheme：A kind of hardening off method of bamboo shoot orchid tissue culture seedling, step are as follows：
A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 2~3 days at room temperature, then placement of uncapping
2~3 days, obtain A product；
B, A product are cleaned, after being soaked with carbendazim solution, dries surface moisture, obtain B product；
C, wood sawdust, fertile soil are mixed and adds rapeseed cake fertilizer as hardening matrix, after hardening matrix sterilization,
Obtain C product；
D, C product are layered on seedbed, B product is then transplanted to hardening 85-95 days on C product.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step a, selected bamboo shoot orchid tissue culture seedling, is seedling
High more than 4cm, 3~5, blade, number 15~30 of taking root, 5~7cm of root long degree.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step b, the concentration of carbendazim solution is dilution 500
2g/L carbendazim solutions again, soak time is 25~35min.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step c, wood sawdust, part by weight humous are
1:1, rapeseed cake fertilizer addition is 1~2kg/100kg hardening matrix.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, the rapeseed cake fertilizer addition are 1.5kg/100kg hardening matrix.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step c, disinfection way is using diluting 200 times
Carbendazim solution carries out disinfection processing.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, it is in described step d, C product are uniform with 20~25cm thickness
It is layered on seedbed.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step d, existed with a diameter of 1.0~1.5cm waddy
The transplanting cave that depth is 2~3cm is beaten on C product, then B product are transplanted in the transplanting cave on C product and carry out hardening.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step d, each transplant transplants 2~3 plants of B in cave
Product.
The hardening off method of foregoing bamboo shoot orchid tissue culture seedling, in described step d, hardening temperature is 15~28 DEG C, and humidity is
70%~85%；It is 60%~70% that C product water content is kept during hardening.
It is compared with the prior art, bamboo shoot orchid tissue culture seedling is placed in preferred hardening matrix by the present invention, in the specific bar of the present invention
Hardening is carried out under part, bamboo shoot orchid tissue culture seedling percent can be improved up to more than 95%；Its speed of growth can be accelerated, in 3 months
Seedling；It is simple to operate and whole process method is simple.
Inventor's comparative analysis 1. wood sawdust, 2. fertile soil, 3. wood sawdust+fertile soil (part by weight 1:1)+vegetable seed
The three kinds of influences of matrix to bamboo shoot orchid tissue culture seedling percent of cake fertilizer (1~2kg/100kg hardenings matrix), survival rate %=are survived
Number/always transplant number × 100%, as a result record is shown for 30 days into live data：Wood sawdust+fertile soil (part by weight 1:1)+dish
Seedcake fertilizer (1~2kg/100kg hardenings matrix) test group, bamboo shoot orchid tissue culture seedling percent highest (being shown in Table 1).
Bamboo shoot orchid tissue culture seedling percent statistical form under the different substrates of table 1
Note：Every 5 days records once survive strain number after bamboo shoot orchid tissue culture seedling replanting to matrix.Temperature is average in same day greenhouse
Temperature.
Embodiment
With reference to embodiment, the present invention is further illustrated, but is not intended as the foundation limited the present invention.
A kind of hardening off method of bamboo shoot orchid tissue culture seedling, step are as follows：
A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 2~3 days at room temperature, then placement of uncapping
2~3 days, obtain A product；Selected bamboo shoot orchid tissue culture seedling is more than height of seedling 4cm, 3~5, blade, number 15~30 of taking root, root long
Spend 5~7cm；
B, A product are cleaned, after being soaked with carbendazim solution, dries surface moisture, obtain B product；The carbendazim solution it is dense
Spend to dilute 500 times of 2g/L carbendazim solutions, soak time is 25~35min；
C, wood sawdust, fertile soil are mixed as hardening matrix, after hardening matrix sterilization, add rapeseed cake fertilizer,
Obtain C product；Described wood sawdust, part by weight humous are 1:1, rapeseed cake fertilizer addition is 1~2kg/100kg hardening bases
Matter, wherein preferable rapeseed cake fertilizer addition is 1.5kg/100kg hardening matrix；The disinfection way is using 200 times of dilution
Carbendazim solution carry out disinfection processing；
D, C product are layered on seedbed, B product is then transplanted to hardening 85-95 days on C product；It is preferred that by C product with 20~25cm
Thickness be uniformly layered on seedbed, with a diameter of 1.0~1.5cm waddy beaten on C product depth be 2~3cm transplanting cave, so
B product are transplanted in the transplanting cave on C product afterwards and carry out hardening, each transplant transplants 2~3 plants of B product in cave；The hardening temperature
Spend for 15~28 DEG C, humidity is 70%~85%；It is 60%~70% that C product water content is kept during hardening.
Embodiment 1
A kind of hardening off method of bamboo shoot orchid tissue culture seedling, step are as follows：
A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 2 days at room temperature, then placement 2 of uncapping
My god, obtain A product；Selected bamboo shoot orchid tissue culture seedling is more than height of seedling 4cm, 3, blade, number 15 of taking root, root long degree 5cm；
B, A product are cleaned up, after being soaked with carbendazim solution, dries surface moisture, obtain B product；The carbendazim solution
Concentration be 500 times of 2g/L carbendazim solutions of dilution, soak time 25min；
C, wood sawdust, fertile soil are mixed as hardening matrix, after hardening matrix sterilization, add rapeseed cake fertilizer,
Obtain C product；Described wood sawdust, part by weight humous are 1:1, rapeseed cake fertilizer addition is 1kg/100kg hardening matrix；Institute
Disinfection way is stated as the processing that carried out disinfection using the carbendazim solution for diluting 200 times；
D, C product are uniformly layered on seedbed with 20cm thickness, it is 2cm that depth is beaten on C product with a diameter of 1.0cm waddy
Transplanting cave, then B product are transplanted in the transplanting cave on C product and carry out hardening, hardening 85 days, each transplant is moved in cave
Plant 2 plants of B product；The hardening temperature is 15 DEG C, humidity 70%；It is 60% that C product water content is kept during hardening.
Embodiment 2
A kind of hardening off method of bamboo shoot orchid tissue culture seedling, step are as follows：
A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 3 days at room temperature, then placement 3 of uncapping
My god, obtain A product；Selected bamboo shoot orchid tissue culture seedling is more than height of seedling 4cm, 5, blade, number 30 of taking root, root long degree 7cm；
B, A product are cleaned up, after being soaked with carbendazim solution, dries surface moisture, obtain B product；The carbendazim solution
Concentration be 500 times of 2g/L carbendazim solutions of dilution, soak time 35min；
C, wood sawdust, fertile soil are mixed as hardening matrix, after hardening matrix sterilization, add rapeseed cake fertilizer,
Obtain C product；Described wood sawdust, part by weight humous are 1:1, rapeseed cake fertilizer addition is 1.5kg/100kg hardening matrix；
The disinfection way is the processing that carried out disinfection using the carbendazim solution for diluting 200 times；
D, C product are uniformly layered on seedbed with 25cm thickness, it is 3cm that depth is beaten on C product with a diameter of 1.5cm waddy
Transplanting cave, then B product are transplanted in the transplanting cave on C product and carry out hardening, hardening 95 days, each transplant is moved in cave
Plant 3 plants of B product；The hardening temperature is 28 DEG C, humidity 85%；It is 70% that C product water content is kept during hardening.
Embodiment 3
A kind of hardening off method of bamboo shoot orchid tissue culture seedling, step are as follows：
A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 2.5 days at room temperature, then placement of uncapping
2.5 days, obtain A product；Selected bamboo shoot orchid tissue culture seedling is more than height of seedling 4cm, 4, blade, number 23 of taking root, root long degree 6cm；
B, A product are cleaned up, after being soaked with carbendazim solution, dries surface moisture, obtain B product；The carbendazim solution
Concentration be 500 times of 2g/L carbendazim solutions of dilution, soak time 30min；
C, wood sawdust, fertile soil are mixed as hardening matrix, after hardening matrix sterilization, add rapeseed cake fertilizer,
Obtain C product；Described wood sawdust, part by weight humous are 1:1, rapeseed cake fertilizer addition is 2kg/100kg hardening matrix；Institute
Disinfection way is stated as the processing that carried out disinfection using the carbendazim solution for diluting 200 times；
D, C product are uniformly layered on seedbed with 22cm thickness, beating depth on C product with a diameter of 1.2cm waddy is
2.5cm transplanting cave, then B product are transplanted in the transplanting cave on C product and carry out hardening, hardening 90 days, each transplant hole
3 plants of B product are transplanted in cave；The hardening temperature is 22 DEG C, humidity 78%；It is 65% that C product water content is kept during hardening.
Claims (10)
- A kind of 1. hardening off method of bamboo shoot orchid tissue culture seedling, it is characterised in that：Step includes：A, choose and grow consistent bamboo shoot orchid tissue culture seedling, in natural light, cover place 2 ~ 3 days at room temperature, then placement 2 ~ 3 of uncapping My god, obtain A product；B, A product are cleaned, after being soaked with carbendazim solution, dries surface moisture, obtain B product；C, wood sawdust, fertile soil are mixed as hardening matrix, after hardening matrix sterilization, adds rapeseed cake fertilizer, obtain C Product；D, C product are layered on seedbed, B product is then transplanted to hardening 85-95 days on C product.
- 2. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：In described step a, institute The bamboo shoot orchid tissue culture seedling of selection, is more than the cm of height of seedling 4,3 ~ 5, blade, number 15 ~ 30 of taking root, the cm of root long degree 5 ~ 7.
- 3. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：It is more in described step b For the concentration of bacterium spirit solution to dilute 500 times of 2g/L carbendazim solutions, soak time is 25 ~ 35 min.
- 4. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：In described step c, saw Wood chip, part by weight humous are 1:1, rapeseed cake fertilizer addition is 1 ~ 2 kg/100kg hardening matrix.
- 5. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 4, it is characterised in that：The rapeseed cake fertilizer addition Measure as 1.5kg/100kg hardening matrix.
- 6. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：In described step c, disappear Malicious mode is the processing that carried out disinfection using the carbendazim solution for diluting 200 times.
- 7. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：, will in described step d C product are uniformly layered on seedbed with 20 ~ 25 cm thickness.
- 8. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：In described step d, use A diameter of 1.0 ~ 1.5cm waddy beats the transplanting cave that depth is 2 ~ 3cm on C product, and B product then are transplanted into the transplanting on C product Hardening is carried out in cave.
- 9. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 8, it is characterised in that：In described step d, often 2 ~ 3 plants of B product are transplanted in individual transplanting cave.
- 10. according to the hardening off method of the bamboo shoot orchid tissue culture seedling described in claim 1, it is characterised in that：In described step d, Hardening temperature is 15 ~ 28 DEG C, and humidity is 70% ~ 85%；It is 60% ~ 70% that C product water content is kept during hardening.
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| CN201710876297.1A CN107493916A (en) | 2017-09-25 | 2017-09-25 | A kind of hardening off method of bamboo shoot orchid tissue culture seedling |
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| CN201710876297.1A CN107493916A (en) | 2017-09-25 | 2017-09-25 | A kind of hardening off method of bamboo shoot orchid tissue culture seedling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771486A (en) * | 2019-11-14 | 2020-02-11 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771486A (en) * | 2019-11-14 | 2020-02-11 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
| CN110771486B (en) * | 2019-11-14 | 2022-03-01 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
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Application publication date: 20171222 |