CN107455259A - A kind of breeding method of beautiful millettia root - Google Patents
A kind of breeding method of beautiful millettia root Download PDFInfo
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- CN107455259A CN107455259A CN201710767249.9A CN201710767249A CN107455259A CN 107455259 A CN107455259 A CN 107455259A CN 201710767249 A CN201710767249 A CN 201710767249A CN 107455259 A CN107455259 A CN 107455259A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention belongs to agricultural plantation technology field.A kind of breeding method of beautiful millettia root, comprises the following steps：A, trimmed between the collection of seed and processing, b, tissue cultures, c, seeding and seedling raising, d, transplanting, e, water and fertilizer management and f, seedling.The breeding method of the beautiful millettia root of the present invention, beautiful millettia root tissue-culturing rapid propagation is carried out by bud Propagation Methods using seed as material, cultivates to obtain seedling by the step of Initial culture, proliferation and subculture culture, strong seedling culture and culture of rootage, seedling is not in de-, the beautiful millettia root that the present invention cultivates grows well, plant height and stalwartness, seedlings root is flourishing, and rattan is sturdy, growth is fast, it is more to tie potato, management is simple, and premunition is strong.
Description
Technical field
The invention belongs to agricultural plantation technology field, and in particular to a kind of breeding method of beautiful millettia root.
Background technology
Beautiful millettia root is Papilionaceae chicken blood Calamus medical and edible dual purpose plant, also known as beautiful millettia, Tianniudali, take off mountain tiger,
Potato, beautiful millettia root, beautiful millettia root are used as medicine with root energetically, and main component is protein, starchiness and alkaloid etc..Beautiful millettia root cures mainly lung
Heat, cough due to deficiency of the lung, pulmonary tuberculosis, rheumatic arthritis, lumbar muscle strain, chronic bronchitis, chronic hepatitis etc., be production it is a variety of in
The primary raw material of patent medicine.It is mainly produced in China Guangdong, Guangxi, Hainan and Vietnam, different beautiful millettia root introduces a collection blades, flower, fruit
And potato form, growing way performance it is different, root system penetration power is strong, and radiation scope is big.Beautiful millettia root is wild mainly by excavating at present, so far
Artificial growth is seldom, greatly develops artificial growth, can both make up wild resource, can create good economic benefits again, have a high potential.
In recent years, it is also gradually deeply and comprehensive to the technical research of beautiful millettia root seedling propagation as the market demand and day increase severely, mainly broadcast
Kind nursery, cuttage and seedling culture and one of tissue cultivating seedling, effective ways of the tissue cultivating seedling as beautiful millettia root biological control, at present
Tender stem segmentses are used as explant, sterilization is relatively difficult, and it is quick to carry out tissue culture by callus approach using seed as material more
Breeding has been studied, there is not yet passing through by material of seed with the report of bud Propagation Methods progress beautiful millettia root tissue-culturing rapid propagation research.
In addition, often there is phenomena such as dead seedling, yellow leaf, transplanting seedling time are long, do not grow in artificial growth beautiful millettia root, beautiful millettia root integral into
Motility rate is not high.In order to promote artificial growth beautiful millettia root, the economic benefit of artificial growth is improved, there is provided a kind of plantation side of beautiful millettia root
Method is people's technical problem urgently to be resolved hurrily.
The content of the invention
Instant invention overcomes the shortcomings that above-mentioned technical problem, there is provided one kind is simple and reasonable, friction ratio is high, energy is firm
Clamp the clamp for carbon block of various shapes.
A kind of breeding method of beautiful millettia root, comprises the following steps：
A, the collection and processing of seed
Growth selection is healthy and strong after fruit maturation, the elite stand of no disease and pests harm, peels off shell after collecting fruit, is used after taking out seed
40~50 DEG C of clear water soaks 2~4 days, then with concentration be 2.5~3.5% it is suitable happy when suspending agent 10~15min of stirring, in nothing
With 0.1~0.2%HgCl on bacterium workbench28~10min of solution disinfection, then 10~15s is soaked with 75~85% ethanol, most
Aseptic water washing is used afterwards 4~6 times, it is standby；
B, tissue cultures
B.1 Initial culture：Seed after step a processing is seeded in germination medium, axenic germination 7~9 days, daily
12~15h of illumination, temperature are 22~26 DEG C；The composition of the germination medium is：DKW+4~6mg/L6-BA+0.2~
0.3mg/L IBA+25g/L sucrose+6g/L agar；
B.2 proliferation and subculture culture：Seed in treating step b.1 sprouts seedling length to 5~6cm, clip top 4cm length band axillary buds
Stem section, 2 sections are cut into, 2 axillary buds of every section of band, traverse is seeded to proliferated culture medium and carries out Multiplying culture, and incubation time is 20~25
My god, obtain proliferation and subculture bud；16~18h of daily illumination, temperature are 23~27 DEG C；
B.3 strong seedling culture：B.2, the proliferation and subculture bud that step obtains is inoculated on strong seedling culture base, the culture medium is put
In carrying out strong seedling culture in culturing room, incubation time is 22~26 days；The strong seedling culture base is MS+0.1~0.4mg/L6-BA
+ 0.2~0.4mg/LNAA+30g/L sucrose+8g/L agar, long 3~4cm healthy and strong Multiple Buds are selected, it is standby；
B.4 culture of rootage：B.3, the healthy and strong Multiple Buds that step is obtained are transferred on root media, and the culture medium is put
In carrying out culture of rootage in culturing room, incubation time is 22~28d, obtains rooted seedling；
C, seeding and seedling raising
C.1 4~5cm Nutrition Soil is initially charged with Seedling bag, spray concentration is 2.5~3.5% on the Nutrition Soil
Carbendazim solution, the rooted seedling program request that b.4 step is obtained covers 0.8~1.2cm's again in the Seedling bag, on upper strata
Nutrition Soil, the Seedling bag after program request are put into warmhouse booth and put into furrow, per the native ridging of furrow surrounding, and are filled with enough water successively；
C.2 when the long unearthed face of seedling whole, when lateral root starts elongation, applied fertilizer, at interval of 15~20d fertilisings once,
The fertilizer of fertilising is calcium and magnesium phosphorus composite fertilizer, and clear water is sprayed after fertilising and cleans blade；
D, transplant
When seedling is grown to 20~30cm, that is, transplanted；
E, water and fertilizer management
At interval of water drenching in 7~10 days once, it is annual to apply fertilizer 3 times, the mixing of calcium and magnesium phosphorus composite fertilizer and farm manure is applied for the first time
Thing, fertilization time be rudiment before, apply calcium and magnesium phosphorus composite fertilizer for the second time, fertilization time is young sprout growth period, applies N P and K for the third time
Composite fertilizer, fertilization time are bud stage；
F, trimmed between seedling：In the overstocked place of seedling growth, weak seedling, sick seedling are removed, retains strong sprout, controls rattan length,
Branch is trimmed successively more than 50-60cm.
Further, the Nutrition Soil is prepared by following methods：By weight, by 100~150 parts of fertile soil, 20
~50 parts of peat soils and 80~100 parts take vermiculite mixing, after being sterilized with 0.5~2.5 part of 35% pentachloronitrobenzene, add
0.2~0.8 part of EM bacteria agent and 0.1~0.3 part of root-inducing powder are mixed evenly, and obtain the Nutrition Soil.
Further, the composition of the proliferated culture medium is：DKW+2~4mg/L6-BA+0.2mg/L IBA+8~10g/L
Cysteine+10g/L agar.
Further, the composition of the root media is：MS+0.2~0.5mg/LIBA+0.3~0.5mg/LNAA+
30g/L sucrose.
Further, Seedling bag is 10cmX10cm degradable Seedling bag in the step c.
Further, during the step d transplantings, selection is sunny, soil layer is deep, draining is good, loose fertile sand
Matter soil, by 18~20cm of line-spacing, 10~15cm of spacing in the rows kinds plant seedling, and planting number is 480~550/mu.
Further, during the step e water and fertilizer managements, the calcium and magnesium phosphorus composite fertilizer's consumption of applying fertilizer for the first time is 30~
45kg/ mus, the amount of application of farm manure is 50~60kg/ mus；The dosage of second of fertilising calcium and magnesium phosphorus composite fertilizer is 60~65kg/
Mu；The dosage of third time fertilising nitrogen-phosphorus-potassium compound fertilizer is 65~70kg/ mus.
In summary, the present invention has the advantages that as a result of such scheme：
1st, a kind of breeding method of beautiful millettia root of the present invention, plant during initial point selection is ripe, can identify and select
To with growing way is healthy and strong, plant type is good, better resistance plant as female parent material, the seedling obtained after being bred by tissue cultures
Can have and maternal plant identical merit.The beautiful millettia root that the present invention cultivates grows well, plant height and stalwartness, children
Seedling well developed root system, rattan is sturdy, and growth is fast, and knot potato is more, and management is simple, and premunition is strong.
2nd, a kind of breeding method of beautiful millettia root of the invention carries out beautiful millettia root group using seed as material by bud Propagation Methods
Training is fast numerous, cultivates to obtain seedling by the step of Initial culture, proliferation and subculture culture, strong seedling culture and culture of rootage, seedling is not
Dehydrating phenomena occurs, without seedling-slowing stage, the hardening time is short, has the characteristics of cultural method is simple, and survival rate is high, suitable for batch
Production.
3rd, the breeding method for the beautiful millettia root that the present invention cultivates, seedling is cultivated by tissue cultures, it is possible to prevente effectively from seed
Because arid dehydration is not germinateed, using Seedling bag seeding and seedling raising, good growing environment can be provided for seedling, what tradition used educates
Seedling bag is smaller, can influence root growth, and root system has grown Seedling bag, does not remove Seedling bag during transplanting, and Root Absorption moisture is limited,
If removing Seedling bag, easily cause the root system of beautiful millettia root seedling to be broken during transplanting, damaged to plant, and the present invention uses
10cmX10cm degradable Seedling bag seeding and seedling raising, can solve above mentioned problem.And the Nutrition Soil warp in Seedling bag
Multiple development test to be crossed to be made, above-mentioned Nutrition Soil is mainly mixed by the fertile soil by sterilizing, peat soil and expense vermiculite, and
Appropriate EM bacteria agent and root-inducing powder are added in Nutrition Soil, makes loosing soil, humus enriches, and nutrition is comprehensive, it is ensured that Niu great
The quality of power.Wherein, fertile soil, peat soil and take and contain substantial amounts of organic matter, N P and K and trace element in leech, have into
Sheet is low, meets the features such as environmental requirement, and EM bacteria agent is grown altogether with symbiosis such as actinomyces in soil, and organic components are sent out through beneficial bacterium
Ferment, energy equilibrated ph value, forms good crop growth environment, improves soil fertilizer matter；Root-inducing powder is used to cultivate seedling, can promote
Take root, shorten rootage duration, improve rooting rate.When seedling is grown to 20~30cm, that is, transplanted, and pass through later stage liquid manure
The step such as trimming between management and seedling, the beautiful millettia root high yield cultivated, stable yields, general rattan branch are received for 2 years one, and every plant can receive 1-2 public affairs
Jin, fresh sweet potato are received for 4 years one, and every plant can receive more than 2 kilograms, and the beautiful millettia root quality better that the present invention cultivates, potato wedge is big, and color is pale, powder
Matter is strong, meets the market demand.
Embodiment
Technical scheme is further described in detail with reference to embodiment.
Embodiment 1
A kind of breeding method of beautiful millettia root, comprises the following steps：The collection of seed and processing, tissue cultures, seeding and seedling raising,
Trimmed between transplanting, water and fertilizer management and seedling.
A, the collection and processing of seed
Growth selection is healthy and strong after fruit maturation, the elite stand of no disease and pests harm, peels off shell after collecting fruit, is used after taking out seed
40 DEG C of clear water soaks 2 days, then with concentration be 2.5% it is suitable happy when suspending agent stirring 10min, used on aseptic working platform
0.1%HgCl28~10min of solution disinfection, then 10s is soaked with 75% ethanol, it is standby finally with aseptic water washing 4 times.
B, tissue cultures
B.1 Initial culture：Seed after step a processing is seeded in germination medium, axenic germination 7 days, per the daylight
According to 12h, temperature is 22 DEG C；The composition of the germination medium is：DKW+4mg/L6-BA+0.2mg/L IBA+25g/L sucrose+
6g/L agar；
B.2 proliferation and subculture culture：Seed in treating step b.1 sprouts seedling length to 5cm, clip top 4cm length band axillary bud stems
Section, is cut into 2 sections, 2 axillary buds of every section of band, and traverse is seeded to proliferated culture medium and carries out Multiplying culture, the proliferated culture medium into
It is divided into：DKW+2mg/L6-BA+0.2mg/L IBA+8g/L cysteine+10g/L agar, incubation time are 20 days, are bred
Subculture bud；Daily illumination 16h, temperature are 23 DEG C；
B.3 strong seedling culture：B.2, the proliferation and subculture bud that step obtains is inoculated on strong seedling culture base, the culture medium is put
In carrying out strong seedling culture in culturing room, incubation time is 22 days；The strong seedling culture base is MS+0.1mg/L6-BA+0.2mg/
LNAA+30g/L sucrose+8g/L agar, long 3cm healthy and strong Multiple Buds are selected, it is standby；
B.4 culture of rootage：B.3, the healthy and strong Multiple Buds that step is obtained are transferred on root media, and the culture medium is put
In carrying out culture of rootage in culturing room, the composition of the root media is：MS+0.2mg/LIBA+0.3mg/LNAA+30g/L
Sucrose, incubation time 22d, obtains rooted seedling.
C, seeding and seedling raising
C.1 4cm Nutrition Soil is initially charged with Seedling bag, Seedling bag is 10cmX10cm degradable Seedling bag, in institute
The carbendazim solution that spray concentration on Nutrition Soil is 2.5% is stated, the rooted seedling program request that b.4 step is obtained is in the Seedling bag
It is interior, 0.8cm Nutrition Soil is covered again on upper strata, and the Seedling bag after program request is put into warmhouse booth and puts into furrow, per furrow surrounding soil
Ridging, and water is filled with enough successively；Wherein, the Nutrition Soil is prepared by following methods：By weight, by 100 parts of fertile soil, 20
Part peat soil and 80 parts takes vermiculite mixing, after being sterilized with 0.5 part of 35% pentachloronitrobenzene, addition 0.2 part of EM bacteria agent and 0.1
Part root-inducing powder is mixed evenly, and obtains the Nutrition Soil.
C.2 when the long unearthed face of seedling whole, when lateral root starts elongation, applied fertilizer, at interval of 15d fertilisings once, applied fertilizer
Fertilizer be calcium and magnesium phosphorus composite fertilizer, after fertilising spray clear water clean blade.
D, transplant
When seedling is grown to 20cm, that is, transplanted, selection is sunny, soil layer is deep, draining is good, loose fertile
Sandy soil, by line-spacing 18cm, spacing in the rows 10cm kinds plant seedling, and planting number is 480/mu.
E, water and fertilizer management
At interval of water drenching in 7 days once, the annual mixture for applying fertilizer 3 times, applying calcium and magnesium phosphorus composite fertilizer and farm manure for the first time, calcium
Magnesium phosphorus composite fertilizer's consumption is 30kg/ mus, and the amount of application of farm manure is 50kg/ mus；Fertilization time be rudiment before, apply calcium and magnesium for the second time
Phosphorus composite fertilizer, the dosage of calcium and magnesium phosphorus composite fertilizer is 60kg/ mus；Fertilization time is young sprout growth period, and it is compound to apply N P and K for the third time
Fertilizer, the dosage of nitrogen-phosphorus-potassium compound fertilizer is 65kg/ mus, and fertilization time is bud stage.
F, trimmed between seedling：In the overstocked place of seedling growth, weak seedling, sick seedling are removed, retains strong sprout, controls rattan length,
Branch is trimmed successively more than 50cm.
Embodiment 2
A kind of breeding method of beautiful millettia root, comprises the following steps：The collection of seed and processing, tissue cultures, seeding and seedling raising,
Trimmed between transplanting, water and fertilizer management and seedling.
A, the collection and processing of seed
Growth selection is healthy and strong after fruit maturation, the elite stand of no disease and pests harm, peels off shell after collecting fruit, is used after taking out seed
50 DEG C of clear water soaks 4 days, then with concentration be 3.5% it is suitable happy when suspending agent stirring 15min, used on aseptic working platform
0.2%HgCl2Solution disinfection 10min, then 15s is soaked with 85% ethanol, it is standby finally with aseptic water washing 6 times.
B, tissue cultures
B.1 Initial culture：Seed after step a processing is seeded in germination medium, axenic germination 9 days, per the daylight
According to 15h, temperature is 26 DEG C；The composition of the germination medium is：DKW+6mg/L6-BA+0.3mg/L IBA+25g/L sucrose+
6g/L agar；
B.2 proliferation and subculture culture：Seed in treating step b.1 sprouts seedling length to 6cm, clip top 4cm length band axillary bud stems
Section, is cut into 2 sections, 2 axillary buds of every section of band, and traverse is seeded to proliferated culture medium and carries out Multiplying culture, the proliferated culture medium into
It is divided into：DKW+4mg/L6-BA+0.2mg/L IBA+10g/L cysteine+10g/L agar, incubation time are 25 days, are increased
Grow subculture bud；Daily illumination 18h, temperature are 27 DEG C；
B.3 strong seedling culture：B.2, the proliferation and subculture bud that step obtains is inoculated on strong seedling culture base, the culture medium is put
In carrying out strong seedling culture in culturing room, incubation time is 26 days；The strong seedling culture base is MS+0.4mg/L6-BA+0.4mg/
LNAA+30g/L sucrose+8g/L agar, long 4cm healthy and strong Multiple Buds are selected, it is standby；
B.4 culture of rootage：B.3, the healthy and strong Multiple Buds that step is obtained are transferred on root media, and the culture medium is put
In carrying out culture of rootage in culturing room, the composition of the root media is：MS+0.5mg/LIBA+0.5mg/LNAA+30g/L
Sucrose, incubation time 28d, obtains rooted seedling.
C, seeding and seedling raising
C.1 5cm Nutrition Soil is initially charged with Seedling bag, Seedling bag is 10cmX10cm degradable Seedling bag, in institute
The carbendazim solution that spray concentration on Nutrition Soil is 3.5% is stated, the rooted seedling program request that b.4 step is obtained is in the Seedling bag
It is interior, 1.2cm Nutrition Soil is covered again on upper strata, and the Seedling bag after program request is put into warmhouse booth and puts into furrow, per furrow surrounding soil
Ridging, and water is filled with enough successively；Wherein, the Nutrition Soil is prepared by following methods：By weight, by 150 parts of fertile soil, 50
Part peat soil and 100 parts takes vermiculite mixing, after being sterilized with 2.5 part of 35% pentachloronitrobenzene, add 0.8 part of EM bacteria agent with
0.3 part of root-inducing powder is mixed evenly, and obtains the Nutrition Soil.
C.2 when the long unearthed face of seedling whole, when lateral root starts elongation, applied fertilizer, at interval of 20d fertilisings once, applied fertilizer
Fertilizer be calcium and magnesium phosphorus composite fertilizer, after fertilising spray clear water clean blade.
D, transplant
When seedling is grown to 30cm, that is, transplanted, selection is sunny, soil layer is deep, draining is good, loose fertile
Sandy soil, by line-spacing 20cm, spacing in the rows 15cm kinds plant seedling, and planting number is 550/mu.
E, water and fertilizer management
At interval of water drenching in 10 days once, the annual mixture for applying fertilizer 3 times, applying calcium and magnesium phosphorus composite fertilizer and farm manure for the first time,
Calcium and magnesium phosphorus composite fertilizer's consumption is 45kg/ mus, and the amount of application of farm manure is 60kg/ mus；Fertilization time be rudiment before, apply calcium for the second time
Mei Lin composite fertilizers, the dosage of calcium and magnesium phosphorus composite fertilizer is 65kg/ mus；Fertilization time is young sprout growth period, applies azophoska for the third time
Hefei, the dosage of nitrogen-phosphorus-potassium compound fertilizer is 70kg/ mus, and fertilization time is bud stage.
F, trimmed between seedling：In the overstocked place of seedling growth, weak seedling, sick seedling are removed, retains strong sprout, controls rattan length,
Branch is trimmed successively more than 60cm.
Embodiment 3
A kind of breeding method of beautiful millettia root, comprises the following steps：The collection of seed and processing, tissue cultures, seeding and seedling raising,
Trimmed between transplanting, water and fertilizer management and seedling.
A, the collection and processing of seed
Growth selection is healthy and strong after fruit maturation, the elite stand of no disease and pests harm, peels off shell after collecting fruit, is used after taking out seed
50 DEG C of clear water soaks 3 days, then with concentration be 3% it is suitable happy when suspending agent stirring 12min, with 0.15% on aseptic working platform
HgCl2Solution disinfection 9min, then 12s is soaked with 80% ethanol, it is standby finally with aseptic water washing 5 times.
B, tissue cultures
B.1 Initial culture：Seed after step a processing is seeded in germination medium, axenic germination 8 days, per the daylight
According to 13h, temperature is 25 DEG C；The composition of the germination medium is：DKW+5mg/L6-BA+0.25mg/L IBA+25g/L sucrose+
6g/L agar；
B.2 proliferation and subculture culture：Seed in treating step b.1 sprouts seedling length to 5.5cm, clip top 4cm length band axillary buds
Stem section, 2 sections are cut into, 2 axillary buds of every section of band, traverse is seeded to proliferated culture medium and carries out Multiplying culture, the proliferated culture medium
Composition is：DKW+3mg/L6-BA+0.2mg/L IBA+9g/L cysteine+10g/L agar, incubation time are 22 days, are increased
Grow subculture bud；Daily illumination 17h, temperature are 25 DEG C；
B.3 strong seedling culture：B.2, the proliferation and subculture bud that step obtains is inoculated on strong seedling culture base, the culture medium is put
In carrying out strong seedling culture in culturing room, incubation time is 22~26 days；The strong seedling culture base is MS+0.1~0.4mg/L6-BA
+ 0.2~0.4mg/LNAA+30g/L sucrose+8g/L agar, long 3.5cm healthy and strong Multiple Buds are selected, it is standby；
B.4 culture of rootage：B.3, the healthy and strong Multiple Buds that step is obtained are transferred on root media, and the culture medium is put
In carrying out culture of rootage in culturing room, the composition of the root media is：MS+0.3mg/LIBA+0.4mg/LNAA+30g/L
Sucrose, incubation time 25d, obtains rooted seedling.
C, seeding and seedling raising
C.1 4.5cm Nutrition Soil is initially charged with Seedling bag, Seedling bag is 10cmX10cm degradable Seedling bag,
Spray concentration is 3% carbendazim solution on the Nutrition Soil, and the rooted seedling program request that b.4 step is obtained is in the Seedling bag
It is interior, 1.1cm Nutrition Soil is covered again on upper strata, and the Seedling bag after program request is put into warmhouse booth and puts into furrow, per furrow surrounding soil
Ridging, and water is filled with enough successively；Wherein, the Nutrition Soil is prepared by following methods：By weight, by 120 parts of fertile soil, 40
Part peat soil and 90 parts takes vermiculite mixing, after being sterilized with 2 part of 35% pentachloronitrobenzene, 0.5 part of EM bacteria agent of addition and 0.2 part
Root-inducing powder is mixed evenly, and obtains the Nutrition Soil.
C.2 when the long unearthed face of seedling whole, when lateral root starts elongation, applied fertilizer, at interval of 18d fertilisings once, applied fertilizer
Fertilizer be calcium and magnesium phosphorus composite fertilizer, after fertilising spray clear water clean blade.
D, transplant
When seedling is grown to 25cm, that is, transplanted, selection is sunny, soil layer is deep, draining is good, loose fertile
Sandy soil, by line-spacing 19cm, spacing in the rows 12cm kinds plant seedling, and planting number is 500/mu.
E, water and fertilizer management
At interval of water drenching in 9 days once, the annual mixture for applying fertilizer 3 times, applying calcium and magnesium phosphorus composite fertilizer and farm manure for the first time, calcium
Magnesium phosphorus composite fertilizer's consumption is 35kg/ mus, and the amount of application of farm manure is 55kg/ mus；Fertilization time be rudiment before, apply calcium and magnesium for the second time
Phosphorus composite fertilizer, the dosage of calcium and magnesium phosphorus composite fertilizer is 62kg/ mus；Fertilization time is young sprout growth period, and it is compound to apply N P and K for the third time
Fertilizer, the dosage of nitrogen-phosphorus-potassium compound fertilizer is 68kg/ mus, and fertilization time is bud stage.
F, trimmed between seedling：In the overstocked place of seedling growth, weak seedling, sick seedling are removed, retains strong sprout, controls rattan length,
Branch is trimmed successively more than 55cm.
Test case
The inventive method passes through a large amount of field tests, takes cottage method, seed direct respectively of a sort beautiful millettia root
Plantation and 1-3 of the embodiment of the present invention breeding method are planted, the average production situation of record beautiful millettia root root block after 3 years,
It is compared again.Beautiful millettia root root block growing state is shown in Table 1.
Table 1
In summary, shown by planting result record data, the breeding method of beautiful millettia root of the present invention and traditional cuttage
The directly plantation of method, seed is compared, and beautiful millettia root length is relatively neat, emergence rate is high, and drought resisting, resistance against diseases are strong after culture transferring, stem section without
Rot, and transplant income height after plantation, and traditional breeding method young plant growth is also irregular, yield income is relatively low.In addition,
The seedling obtained after being bred using the present invention by tissue cultures is planted, and beautiful millettia root grows well, plant height and strong
Strong, seedlings root is flourishing, and rattan is sturdy, and growth is fast, and knot potato is more, and premunition is strong.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of breeding method of beautiful millettia root, it is characterised in that comprise the following steps：
A, the collection and processing of seed
Growth selection is healthy and strong after fruit maturation, the elite stand of no disease and pests harm, and shell is peeled off after collecting fruit, take out after seed with 40~
50 DEG C of clear water soaks 2~4 days, then with concentration be 2.5~3.5% it is suitable happy when suspending agent 10~15min of stirring, in sterile work
Make on platform with 0.1~0.2%HgCl28~10min of solution disinfection, then 10~15s is soaked with 75~85% ethanol, finally use
Aseptic water washing 4~6 times, it is standby；
B, tissue cultures
B.1 Initial culture：Seed after step a processing is seeded in germination medium, axenic germination 7~9 days, daily illumination
12~15h, temperature are 22~26 DEG C；The composition of the germination medium is：DKW+4~6mg/L6-BA+0.2~0.3mg/L
IBA+25g/L sucrose+6g/L agar；
B.2 proliferation and subculture culture：Seed in treating step b.1 sprouts seedling length to 5~6cm, clip top 4cm length band axillary bud stems
Section, 2 sections being cut into, 2 axillary buds of every section of band, traverse is seeded to proliferated culture medium and carries out Multiplying culture, and incubation time is 20~25 days,
Obtain proliferation and subculture bud；16~18h of daily illumination, temperature are 23~27 DEG C；
B.3 strong seedling culture：B.2, the proliferation and subculture bud that step obtains is inoculated on strong seedling culture base, the culture medium is placed in training
Indoor carry out strong seedling culture is supported, incubation time is 22~26 days；The strong seedling culture base is MS+0.1~0.4mg/L6-BA+0.2
~0.4mg/LNAA+30g/L sucrose+8g/L agar, long 3~4cm healthy and strong Multiple Buds are selected, it is standby；
B.4 culture of rootage：B.3, the healthy and strong Multiple Buds that step is obtained are transferred on root media, and the culture medium is placed in into training
Indoor carry out culture of rootage is supported, incubation time is 22~28d, obtains rooted seedling；
C, seeding and seedling raising
C.1 4~5cm Nutrition Soil is initially charged with Seedling bag, on the Nutrition Soil spray concentration be 2.5~3.5% it is more
Bacterium spirit solution, the rooted seedling program request that b.4 step is obtained cover 0.8~1.2cm nutrition again in the Seedling bag, on upper strata
Soil, the Seedling bag after program request are put into warmhouse booth and put into furrow, per the native ridging of furrow surrounding, and are filled with enough water successively；
C.2 when the long unearthed face of seedling whole, when lateral root starts elongation, applied fertilizer, at interval of 15~20d fertilisings once, applied fertilizer
Fertilizer be calcium and magnesium phosphorus composite fertilizer, after fertilising spray clear water clean blade；
D, transplant
When seedling is grown to 20~30cm, that is, transplanted；
E, water and fertilizer management
At interval of water drenching in 7~10 days once, it is annual to apply fertilizer 3 times, the mixture of calcium and magnesium phosphorus composite fertilizer and farm manure is applied for the first time, is applied
The fertile time be rudiment before, apply calcium and magnesium phosphorus composite fertilizer for the second time, fertilization time is young sprout growth period, and it is compound to apply N P and K for the third time
Fertilizer, fertilization time are bud stage；
F, trimmed between seedling：In the overstocked place of seedling growth, weak seedling, sick seedling are removed, retains strong sprout, controls rattan length, branch
Trimmed successively more than 50-60cm.
A kind of 2. breeding method of beautiful millettia root according to claim 1, it is characterised in that：The Nutrition Soil is by following sides
It is prepared by method：By weight, 100~150 parts of fertile soil, 20~50 parts of peat soils and 80~100 parts are taken into vermiculite to mix,
After being sterilized with 0.5~2.5 part of 35% pentachloronitrobenzene, add 0.2~0.8 part of EM bacteria agent and 0.1~0.3 part of root-inducing powder mixing is stirred
Mix uniformly, obtain the Nutrition Soil.
A kind of 3. breeding method of beautiful millettia root according to claim 1, it is characterised in that：The composition of the proliferated culture medium
For：DKW+2~4mg/L6-BA+0.2mg/L IBA+8~10g/L cysteine+10g/L agar.
A kind of 4. breeding method of beautiful millettia root according to claim 1, it is characterised in that：The composition of the root media
For：MS+0.2~0.5mg/LIBA+0.3~0.5mg/LNAA+30g/L sucrose.
A kind of 5. breeding method of beautiful millettia root according to claim 1, it is characterised in that：Seedling bag is in the step c
10cmX10cm degradable Seedling bag.
A kind of 6. breeding method of beautiful millettia root according to claim 1, it is characterised in that：During the step d transplantings, selection
It is sunny, soil layer is deep, draining is good, loose fertile sandy soil, by 18~20cm of line-spacing, 10~15cm of spacing in the rows kinds are planted
Seedling, planting number are 480~550/mu.
A kind of 7. breeding method of beautiful millettia root according to claim 1, it is characterised in that：The step e water and fertilizer management mistakes
Cheng Zhong, calcium and magnesium phosphorus composite fertilizer's consumption of applying fertilizer for the first time is 30~45kg/ mus, and the amount of application of farm manure is 50~60kg/ mus；Second
The dosage of secondary fertilising calcium and magnesium phosphorus composite fertilizer is 60~65kg/ mus；The dosage of third time fertilising nitrogen-phosphorus-potassium compound fertilizer is 65~70kg/
Mu.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108101610A (en) * | 2018-01-30 | 2018-06-01 | 东兰县委荣村伟造林下药材种植合作社 | A kind of special root system fertilizer of beautiful millettia root and preparation method |
| CN111280004A (en) * | 2019-11-26 | 2020-06-16 | 广西壮族自治区农业科学院 | Asparagus cochinchinensis planting method |
| CN112335509A (en) * | 2020-10-26 | 2021-02-09 | 广东省中药研究所 | Cultivation method of beautiful millettia root |
| CN113396820A (en) * | 2021-02-23 | 2021-09-17 | 广州麦林种业有限公司 | Method for cultivating new variety of beautiful millettia root by molecular marker crossbreeding method |
| CN114902924A (en) * | 2022-04-26 | 2022-08-16 | 广西富睿思科技有限公司 | Planting method of beautiful millettia root |
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| CN106688893A (en) * | 2017-01-15 | 2017-05-24 | 黄振忠 | Beautiful millettia root tissue culture seedling raising method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101610A (en) * | 2018-01-30 | 2018-06-01 | 东兰县委荣村伟造林下药材种植合作社 | A kind of special root system fertilizer of beautiful millettia root and preparation method |
| CN111280004A (en) * | 2019-11-26 | 2020-06-16 | 广西壮族自治区农业科学院 | Asparagus cochinchinensis planting method |
| CN112335509A (en) * | 2020-10-26 | 2021-02-09 | 广东省中药研究所 | Cultivation method of beautiful millettia root |
| CN113396820A (en) * | 2021-02-23 | 2021-09-17 | 广州麦林种业有限公司 | Method for cultivating new variety of beautiful millettia root by molecular marker crossbreeding method |
| CN114902924A (en) * | 2022-04-26 | 2022-08-16 | 广西富睿思科技有限公司 | Planting method of beautiful millettia root |
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