CN102948322B - Seed propagation method for quickly obtaining healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance - Google Patents
Seed propagation method for quickly obtaining healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance Download PDFInfo
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Abstract
The invention discloses a seed propagation method for quickly obtaining healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance, comprising a step one of performing change-temperature treatment for promotion of embryos maturity; a step two of disposing with a growth regulator to release seed dormancy and accelerating sprouting; and a step three of soaking and sowing the seeds of notopterygium incisum Ting ex H. T. Chang with dormancy release in nutrients to accelerate sprouting of healthy sprouts; after the dormancy breaking, soaking the seeds for 2h in 0.2wt% of mixed liquor of 0.8 to 1.0wt% of monopotassium phosphate and Feilong-brand micro element fertilizer 'Feilong-1'; sowing in a seedbed containing 60% of humus and covering covered with 1-2cm fine earth; and waiting for sprouts and seedlings. The seed propagation method for quickly obtaining the healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance causes the propagation of the healthy seedlings in abundance from the seeds of the notopterygium incisum Ting ex H. T. Chang to be solved, and has the advantages of short propagation time and high propagation efficiency; in addition, the survival seedlings are healthy; and therefore, the great demand of artificial notopterygium incisum Ting ex H. T. Chang cultivation on the seedlings is solved quite well.
Description
Technical field
The cultivation of biology involved in the present invention, belong to notopterygium root (Notopterygium incisum Ting ex H.T.Chang) standardization production technical standard operational procedure (SOP) field, particularly a kind of seed propagation method that obtains in a large number, fast healthy and strong notopterygium root seedling.
Background technology
Notopterygium root is the perennial wild traditional Chinese medicine of Umbelliferae (Umbelliferae), is China endemic species, one of medicinal herbs most in use in Ye Shizhongzang Qiang medicine.Notopterygium root is used as medicine with root-like stock and root, can inducing sweat and dispelling exogenous evils, again can wines used as antirheumatic and pain relieving, and taste is pungent, warm in nature.It is mainly distributed in the high and cold mountain area that economize in Sichuan, Qinghai, Gansu and Tibet etc., always relies on wild excavating.Predatoriness is in recent years excavated and habitat destruction, causes each Genuine producing area to face the threat of resource exhaustion and germ plasm resource forfeiture, has been put into national III level protective plant, and has entered national endangered species Red List.The fundamental way of protection wild resource and solution imbalance between supply and demand is to realize scaled artificial planting.But the artificial planting of notopterygium root scale at present is also faced with the bottleneck of many technical bottlenecks, particularly sapling multiplication.In production, notopterygium root sapling multiplication is used rhizome vegetative propagation and seed sexual propagation conventionally, vegetative propagation efficiency is low, be not easy to obtain seedling in enormous quantities, sexual propagation reproductive efficiency is high, but because notopterygium root seed has the after-ripening of embryo form and physiology after-ripening double combination obstacle, slow long and difficult so germinate, germination rate is low.Therefore inventing a kind of seed propagation method that improves notopterygium root percentage of seedgermination, obtains fast a large amount of, healthy and strong notopterygium root seedling, is the key link that realizes notopterygium root manual scale planting.
Summary of the invention
The object of this invention is to provide a kind of seed propagation method that obtains in a large number, fast healthy and strong notopterygium root seedling, to solve a difficult problem for notopterygium root scaled artificial planting seedling shortage.
Technical scheme of the present invention is:
With field acquisition ripe uncracked notopterygium root seed then, obtain seed and remove after flat grain, empty grain, worm food grain, with the mercuric chloride soaking disinfection of 0.1wt%, then carry out following processing:
The first step is carried out alternating temperature processing, promotes embryo maturation.First be put in 20 ℃ insulating box process 5 days, and then be put in 4 ℃ refrigerator process 5 days, so repeatedly alternating temperature process 5-6 time, promotion embryo maturation.
Second step, growth regulator processing, removes seed dormancy, carries out vernalization.After alternating temperature is processed, with the 6-BA immersion treatment notopterygium root seed of 10mg/L concentration after 1.5 hours, take out notopterygium root seed and be placed in germination alms bowl, wetting filter paper in upper cover, keep seed moistening, then germination alms bowl is placed on to vernalization 20-30 days in 15 ℃ of incubators of temperature.
The 3rd step, carries out nutrition seed soaking, sowing to the notopterygium root seed of breaking dormancy, promotes to send out bud strong.After seed breaking dormancy, with the potassium dihydrogen phosphate of 0.8-1.0wt% and the mixed liquor of the micro-fertilizer of flying dragon board " flying dragon No. 1 " 0.2wt% seed soaking 2h, be then seeded in the seedbed of humus soil content 60%, cover the fine earth of 1-2cm, wait for germination and emergence.
Described notopterygium root is the Original plant of notopterygium root, belongs to Umbelliferae herbaceos perennial notopterygium root (Notopterygium incisum Ting ex H.T.Chang), but not notopterygium forbesii (Notopterygium forbesii Boiss).
The seed germinateing for notopterygium root must be taken from ripe and uncracked fruit, needs through screening after obtaining seed, rejects flat grain, empty grain, worm food grain etc. and loses germinating capacity or the weak seed of germinating capacity.
Notopterygium root seed needs disinfection before germinateing, and the disease reducing in germination process infects.Mercuric chloride with 0.1wt% soaks sterilizing 5 ~ 8 minutes, and aseptic deionized water is standby after rinsing 3 ~ 4 times.
Because notopterygium root seed exists the double grading of the after-ripening of embryo form and physiology after-ripening, therefore in seed treatment process, must first carry out the alternating temperature processing of high and low temperature, then use HORMONE TREATMENT, processing sequence can not be put upside down, the effect of hormone is just obvious like this, and the time of breaking dormancy could shorten greatly.
Notopterygium root is shady plant garden, to high light sensitivity, while therefore growing seedlings in seedbed, after seed breaks ground and emerges, should put up a shed and shade, and available branch or sunshade net shade, and shading rate is at 30%-40%.
For obtaining healthy and strong seedling, after seed breaking dormancy, need to carry out seed soaking, treatment fluid must comprise the micro-element nutritions such as nitrogen, phosphorus, potassium macro-element nutrients and zinc, boron, and this programme is used the mixed liquor of potassium dihydrogen phosphate and the micro-fertilizer of flying dragon board " flying dragon No. 1 " 0.2% effective.Concentration for the treatment of is crucial, can not be too high, otherwise easily wound is planted, low poor effect, the concentration of this programme is for using potassium dihydrogen phosphate 0.8-1.0%, micro-fertilizer " flying dragon No. 1 " 0.2%, concentration height shorten seed soaking time, concentration is low extends seed soaking time, can reach corresponding effect.
The invention has the advantages that:
(1) adopt the method that alternating temperature is processed and HORMONE TREATMENT combines, can improve generation and the activity of α-amylase in seed, promote cell division, can be good at promoting the maturation of notopterygium root embryo, breaking dormancy.
(2) the method adopts potassium dihydrogen phosphate and micro-fertile seed soaking, can obtain healthy and strong notopterygium root seedling, has improved planting percent, has improved the survival rate that large area is planted simultaneously.
(3) solved with notopterygium root seed fast, the method in amount reproduction strong sprout, have advantages of that repoductive time is fast, reproductive efficiency is high, seedling is healthy and strong, can solve the artificial planting notopterygium root demand a large amount of to seedling.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
1, collection screen seed selection: field acquisition late September to mid-October is ripe uncracked notopterygium root (Notopterygium incisum Ting ex H.T.Chang) seed then, removes flat grain, empty grain, worm food grain.
2, seed disinfection, the disease reducing in germination process infects: the mercuric chloride with 0.1wt% soaks sterilizing 5 ~ 8 minutes, and aseptic deionized water is standby after rinsing 3 ~ 4 times.
3, alternating temperature is processed seed, promotes embryo maturation: the insulating box that is first put in 20 ℃ is processed 5 days, and then is put in the refrigerator of 4 ℃ and processes 5 days, and alternating temperature is processed 5-6 time so repeatedly, the maturation of promotion embryo.
4, HORMONE TREATMENT is removed seed dormancy: after alternating temperature is processed, with the 6-BA immersion treatment notopterygium root seed of 10mg/L concentration, after 1.5 hours, be placed in germination alms bowl wetting filter paper in upper cover, keep seed moistening, then germination alms bowl is placed on to vernalization 20-30 days in 15 ℃ of incubators of temperature.
5, seed nutrition seed soaking: after seed breaking dormancy, with the potassium dihydrogen phosphate of 0.8-1.0% and the mixed liquor of the micro-fertilizer of flying dragon board " flying dragon No. 1 " 0.2% seed soaking 2h.
6, sowing is germinateed: the planting seed after seed soaking, in the seedbed of humus soil rich content, is covered to the fine earth of 1-2cm, wait for germination and emergence, germination rate can reach more than 70%.
7, shade after emerging: after seed breaks ground and emerges, scaffolding shades, and available branch or sunshade net shade, and shading rate is at 30%-40%.
The method solved with notopterygium root seed fast, the method in amount reproduction strong sprout, have advantages of that repoductive time is fast, reproductive efficiency is high, seedling is healthy and strong, the seed next year gathering autumn then can be emerged and be obtained healthy and strong seedling, can solve the wilderness demand of artificial planting notopterygium root to seedling.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. a seed propagation method that obtains in a large number, fast healthy and strong notopterygium root seedling, is characterized in that: take field acquisition then ripe uncracked notopterygium root seed as material, remove after flat grain, the first step is carried out alternating temperature and is processed and promote embryo maturation; First be put in 20 ℃ insulating box process 5 days, and then be put in 4 ℃ refrigerator process 5 days, so repeatedly alternating temperature process 5-6 time, promotion embryo maturation; Second step, growth regulator processing, removes seed dormancy, carries out vernalization; After alternating temperature is processed, with the 6-BA immersion treatment notopterygium root seed of 10mg/L concentration, after 1.5 hours, be placed in germination alms bowl, wetting filter paper in upper cover, keeps seed moistening, then germination alms bowl is placed on to vernalization 20-30 days in 15 ℃ of incubators of temperature; The 3rd step, carries out nutrition seed soaking, sowing to the notopterygium root seed of breaking dormancy, promotes to send out bud strong; After seed breaking dormancy, with the potassium dihydrogen phosphate of 0.8-1.0wt% and the mixed liquor of the micro-fertilizer of flying dragon board " flying dragon No. 1 " 0.2wt% seed soaking 2h, be then seeded in the seedbed of humus soil content 60%, cover the fine earth of 1-2cm, wait for germination and emergence; Described notopterygium root is the Original plant of notopterygium root, belongs to Umbelliferae herbaceos perennial notopterygium root (Notopterygium incisum Ting ex H.T.Chang).
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688358A (en) * | 2015-07-23 | 2017-05-24 | 中国中医科学院中药研究所 | Rapid germination of Chinese herb notopterygium forbesii seeds |
| CN106489643A (en) * | 2016-10-19 | 2017-03-15 | 莱州市宏顺梅花种植科技有限公司 | A kind of Prunus mume var.bungo Makino breeding method |
| CN107493723A (en) * | 2017-08-07 | 2017-12-22 | 六安栀子农业科技有限公司 | A kind of processing method of salvia seeds |
| CN109831957A (en) * | 2017-09-07 | 2019-06-04 | 陇南远大生物科技有限公司 | A kind of high yield cultivating method of notopterygium forbesii |
| CN108849499A (en) * | 2018-05-28 | 2018-11-23 | 大连根特生物工程技术有限公司 | A kind of expanding propagation method of the method for preparing Rhizoma Et Radix Notopterygii artificial seed and artificial seed |
| CN109041629A (en) * | 2018-06-21 | 2018-12-21 | 四川千草生物技术股份有限公司 | A kind of Rhizoma Et Radix Notopterygii seed afterripening processing method |
| CN108719072A (en) * | 2018-06-21 | 2018-11-02 | 四川千草生物技术股份有限公司 | Rhizoma Et Radix Notopterygii seed tissue culture fast breeding technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1918972A (en) * | 2005-08-22 | 2007-02-28 | 蒋舜媛 | Angelica tissue culture breeding method |
| CN101049060A (en) * | 2007-04-30 | 2007-10-10 | 四川天农现代中药科技开发有限责任公司 | Breaking sleep and budding of notopterygium seeds |
| CN101416577A (en) * | 2008-11-27 | 2009-04-29 | 陇西中天药业有限责任公司 | Notopterygium forbesii seeds treatment and culture method |
| CN101427680A (en) * | 2007-11-10 | 2009-05-13 | 西宁集源生物科技有限公司 | Notopterygium incisium seed processing method |
-
2012
- 2012-11-19 CN CN201210466726.5A patent/CN102948322B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1918972A (en) * | 2005-08-22 | 2007-02-28 | 蒋舜媛 | Angelica tissue culture breeding method |
| CN101049060A (en) * | 2007-04-30 | 2007-10-10 | 四川天农现代中药科技开发有限责任公司 | Breaking sleep and budding of notopterygium seeds |
| CN101427680A (en) * | 2007-11-10 | 2009-05-13 | 西宁集源生物科技有限公司 | Notopterygium incisium seed processing method |
| CN101416577A (en) * | 2008-11-27 | 2009-04-29 | 陇西中天药业有限责任公司 | Notopterygium forbesii seeds treatment and culture method |
Non-Patent Citations (6)
| Title |
|---|
| 史静等.羌活种子发芽及实生苗生长发育的研究.《中国中药杂志》.2007,第32卷(第18期), |
| 张恩和等.野生羌活种子休眠机理及破除休眠技术研究.《草地学报》.2007,第15卷(第6期), |
| 羌活种子发芽及实生苗生长发育的研究;史静等;《中国中药杂志》;20070930;第32卷(第18期);第1841-1844页 * |
| 羌活规范化栽培技术;陈永刚等;《甘肃农业》;20110131(第1期);第90-91页 * |
| 野生羌活种子休眠机理及破除休眠技术研究;张恩和等;《草地学报》;20071130;第15卷(第6期);第509-514页 * |
| 陈永刚等.羌活规范化栽培技术.《甘肃农业》.2011,(第1期), |
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