CN103609223A - Method Paris polyphylla var.chinensis through gibberellin and wet sand stratification - Google Patents

Method Paris polyphylla var.chinensis through gibberellin and wet sand stratification Download PDF

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Publication number
CN103609223A
CN103609223A CN201310342231.6A CN201310342231A CN103609223A CN 103609223 A CN103609223 A CN 103609223A CN 201310342231 A CN201310342231 A CN 201310342231A CN 103609223 A CN103609223 A CN 103609223A
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seed
seeds
wet sand
paris polyphylla
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CN103609223B (en
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宋发军
刘学群
王春台
黄宗华
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South Central University for Nationalities
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Abstract

The invention provides a The concrete steps comprises: picked seeds are subjected to pretreatment, immersion with distilled water, treatment of the seeds with a gibberellin solution, disinfection of the seeds, wet sand variable temperature stratification treatment of the seeds for 240 days; the seeds after the wet sand variable temperature stratification treatment are planted in soil directly, or the seeds after the wet sand variable temperature stratification treatment are remained in the wet sand and the seeds are cultured for 120 days at a constant temperature of 18-22 DEG C; the radicles of the seeds grow into taproots after lignification; then the germinated seeds are planted in a field for breeding. Compared with existing seedling raising technologies for Paris polyphylla var.chinensis, the method realizes that the germination time is shortened greatly when high germination rate is ensured. In the method, the seeds after germination in a lab are planted in a big field directly, the seeds can emerge after 1 year, the germination rate can reach 98.51%, and the emergence rate of the seeds planted in a field is above 98%.

Description

The long-pending method that paris polyphylla seed quick-speed germination is bred of gibberellin and wet sand bed
Technical field
The present invention relates to a kind ofly by the long-pending method that paris polyphylla seed quick-speed germination is bred of gibberellin and wet sand bed, belong to planting technical field.
Background technology
Paris polyphylla (Paris polyphylla var.chinensis) makes again Paris polyphylla, schefflera arboricola, flea stop etc., what as far back as < < Sheng Nong's herbal classic > >, record is the name " flea stops " with medicinal part root-like stock, it is the general name (Chinese Pharmacopoeia Commission, 2005) of Liliaceae (Liliaceae) Paris (Paris) herbaceos perennial.
The plant height of paris polyphylla is 30 ~ 100cm, and 5 ~ 11 pieces, blade is shaped as oval shape lanceolar, narrow avette, and a yellowish green peanut is longer than the stem top of verticillate leaves sheet; The single vertical growth of stem, is white green and aubergine more, is generally 275 ~ 376 days the period of growing; Brown, there is oblique link, grow wild, loose and normal crooked root-like stock grow in underground, diameter 1.3 ~ 3cm, long 3 ~ 8cm, has stem residue or the bud trace of depression, and has contractile root on its top; Florescence is generally in 5 ~ July; The fruit phase, fruit was capsule in 8 ~ October, and capsule splits, and seed is most red meat chylema fruit, and seed is from breeding medicinal needs 5 ~ 10 years, and resource regeneration is very slow.Cause the resource of medicinal wild paris polyphylla to be on the verge of exhaustion.Therefore, Chinese scholars starts the introducing and planting of extensive concern paris polyphylla, the tissue of different explants is cultivated and the fast-germination technology of seed.
In prior art, to study paris polyphylla modes of reproduction be mainly sexual propagation and nourish and generate.Paris polyphylla seed has the characteristic of " secondary dormancy ", makes its breeding cycle long, and the key of experiment is to break seed dormancy, impels seed quick-speed germination.Adopt at present the rhizome cutting propagation of more rhizome, not only consumed medicinal part, and easily cause germ plasm resource to degenerate, resistance reduces.Research shows, high temperature and low temperature can suppress the sprouting of Paris polyphylla seed and the growth of radicle, and the low temperature of 9 ℃, its root of hair rate is 0.The topology discovery of research paris polyphylla seed, the embryo differentiation of mature seed not exclusively, seed has the physiological property of " secondary dormancy ", if use separately high temperature and low temperature, seed dormant releasing is not had to effect, only have by the alternate combinations of high temperature and low temperature, just can break the dormancy of seed, promote the sprouting of seed and grow seedling.Alternately there is obvious facilitation 22 ℃ of the temperature of Yunnan Rhizoma Paridis earthing of seeds and number of days are combined as 14 days 4 ℃ and 14 days to breaking dormancy in totally 112 days.Chinese scholars is to adopt the lamination mode of alternating temperature to process seed to the research major part of paris polyphylla seed at present, and breaking dormancy, promotes seed quick-speed germination.Conventional plant hormone is as GA 3, CTK, growth hormone and chemical agent PEG have obvious facilitation to the sprouting of different seeds, research is extensively and receive much concern.
Research to paris polyphylla seed is also found, pluck the paris polyphylla seed of ripe cracking annual 9 ~ October, seed is placed in to 4 ℃ of preservations of low temperature after removing red exosper, in 1 year as a result, during experiment, seed can be taken at any time, and this just brings very large convenience to test.But, by the detection test to new seed and year old seeds germination rate, to find, the germination rate of new seed is higher than the year old seeds of preserving 1 year.Seed stores at cryogenic conditions, although may cause the variation of intracellular some Physiology and biochemistry or break the physiological equilibrium of embryo, thereby can strengthen the ability of the dedifferentiation of seed, but the time of earthing of seeds is excessively of a specified duration, also in possibility cell, the physiological structure of some enzyme is destroyed, thereby has affected the germination rate of seed.
Can find out from the above analysis, there is following deficiency in paris polyphylla seed germination propagation technique at present: the one, sprout repoductive time longer, and the 2nd, germination rate is not high, and the 3rd, be not suitable for large-scale production.Therefore be necessary the technology that research and development better impel paris polyphylla seed quick-speed germination to breed.
Technical scheme
For overcoming in existing paris polyphylla seed germination technology, the shortcoming that seed treatment troublesome poeration and time are long, the invention provides a kind of gibberellin and processes with wet husky alternating temperature stratification the method that paris polyphylla seed quick-speed germination is bred.The method is compared with the seedling growing process of existing paris polyphylla, having realized sprout time in the situation that guaranteeing that germination rate is higher shortens dramatically, and use the inventive method to be directly seeded into land for growing field crops after sprouting in laboratory, germination rate can reach 98.51%, and plantation is arrived field emergence rate up to more than 98%.
Realizing the technical scheme that the object of the invention adopts is that concrete steps are as follows with processing with gibberellin and wet husky alternating temperature stratification the method that paris polyphylla seed quick-speed germination is bred:
(1) seed of plucking is carried out to pretreatment: the ripe paris polyphylla seed of plucking is removed after clever shell, the sealing of dress sealed bag, put into 0 ~ 4 ℃ of preservation, when observing, when starting to decay, takes out the exosper of seed seed, red exosper is removed in stranding, remaining milky seed, dries moisture, be positioned over 0 ~ 4 ℃ standby;
(2) by Gibberellins solution, process seed: under the constant incubator and low light condition of 18 ~ 22 ℃, first use distilled water immersion seed 24 ~ 48h; By the Gibberellins solution that concentration is 200 mg/L~500 mg/L, soak milky seed again, outwell Gibberellins solution after soaking 24 ~ 48h, water rinses the seed after soaking, until clean residual gibberellin;
(3) under aseptic condition, be seed sterilizing: in superclean bench, first use aseptic washing seed 3 ~ 5 times, service property (quality) percentage concentration 70 ~ 80% alcohol disinfecting 30 ~ 45s then, aseptic washing 3 ~ 5 times, use again mass percentage concentration 0.1 ~ 0.15% mercuric chloride to soak 10 ~ 15 minutes, aseptic washing 3 ~ 5 times.
(4) wet husky alternating temperature stratification of seed is processed: by sterilizing completely seed bury in wet sand, by burying seed in wet sand, be placed in turn under 0 ~ 4 ℃, 18 ~ 22 ℃, 0 ~ 4 ℃, 18 ~ 22 ℃ conditions and respectively cultivate respectively 45 ~ 60 days, wetly husky alternating temperature stratification total time reach 180 ~ 240 days;
(5) sprout and breed: the seed after directly wet husky alternating temperature stratification being processed is in soil, or the seed after wet husky alternating temperature stratification is processed is stayed in wet sand, under 18 ~ 22 ℃ of conditions, continue constant temperature culture 90 ~ 120 days, the radicle lignification of seed develops into main root, then the seed of sprouting is planted to field and breeds.
It is first with distilled water seed soaking 24 ~ 48h before step (2) is processed seed by Gibberellins solution that technique scheme is further limited; (4) wet husky alternating temperature stratification.
The present invention processes by the seed of harvesting being removed to pretreatment, the distilled water immersion of exosper as shown from the above technical solution, after Gibberellins solution immersion treatment, sterilization treatment, the wet husky alternating temperature stratification that seed is carried out 180 ~ 240 days is processed, seed after finally directly wet husky alternating temperature stratification being processed makes it breed in soil, or the seed after wet husky alternating temperature stratification is processed is stayed in wet sand, under 18 ~ 22 ℃ of conditions, continue constant temperature culture 90 ~ 120 days, the radicle lignification of seed develops into main root, then the seed of sprouting is planted to field and breeds.Through experiment statistics, adopt this method, seed germination rate reaches as high as 98.51%, and plantation is emerged then to field, and emergence rate reaches more than 98%.
Visible, the present invention is to provide the paris polyphylla seminal propagation technology that a kind of sprout time is short, germination rate is high.After sprouting in laboratory, be directly seeded into land for growing field crops, just can emerge then.Compare with other the seedling growing process of paris polyphylla, sprout time shortens dramatically.Spring sowing then 7, just can emerge August, for the production of paris polyphylla medicinal material, at nursery stage, can save the more than 1 year time, has great importance and economic worth in production practices.
Embodiment
By the seed of the paris polyphylla maturation of plucking, artificial removal's grain husk shell, pack plastic sack into, with rope, tie, put into 4 ℃, laboratory refrigerator, the exosper of a period of time seed starts to decay, with hand rubbing, remove red exosper, and remove exosper with fine ga(u)ge screen sieve, remaining is milky seed, dry moisture, be placed on 4 ℃ of refrigerators standby.
Get paris polyphylla seed, by Gibberellins solution, soak 48h, distilled water compares.Seed is buried by a certain percentage in the wet sand of 150mm culture dish, then being placed on 0 ~ 4 ℃ of condition processes 60 days, temperature being transferred to 18 ~ 20 ℃ places 60 days again, be adjusted to again 0 ~ 4 ℃ of low temperature 60 days, being placed on 20 ~ 22 ℃ processes 60 days, seed is cultivated after 240 days by damp sand alternating temperature stratification, and a part directly sowing is sprouted in soil, and another part seed continues lamination in wet sand.
Seed for direct sowing in soil, 100mg/LGA 3the germination rate that solution soaks 48h seed is 95.35%; 150mg/LGA 3the germination rate that soaks seed 48h is 98.51%; 600mg/LGA 3soaking seed 48h germination rate is only 55.81%.
For continuing to stay seed in wet sand, 18~22 ℃ of constant temperature culture, after 120 days, the radicle of seed lignification develops into main root, the kind shell of some seeds comes off, the cotyledon that exposes parcel, differentiates lateral bud at each rhizome position, plants the seed in sand, the nutrition of endosperm exhausts gradually, kind of skin becomes black gradually, naturally comes off, and obviously like this sees cotyledon.
The paris polyphylla seed of sprouting is above planted to field, and after 2 months, root-like stock forms, and emerges neat after 4 months, and emergence rate reaches more than 98%, and upgrowth situation is good.
Known by embodiment, the invention provides the technology of the paris polyphylla seminal propagation that a kind of sprout time is short, germination rate is high.After sprouting in laboratory, be directly seeded into land for growing field crops, within 1 year, just can emerge, germination rate can reach 98.51%, and plantation is arrived field emergence rate up to more than 98%.The scaled artificial planting that the present invention can be paris polyphylla provides stable germ plasm resource, guarantees success and the economic benefit of artificial cultivation plantation.

Claims (2)

1. the method that gibberellin and wet husky alternating temperature stratification are bred paris polyphylla seed quick-speed germination, is characterized in that adopting following steps:
(1) seed of plucking is carried out to pretreatment: the ripe paris polyphylla seed of plucking is removed after clever shell, the sealing of dress sealed bag, put into 0 ~ 4 ℃ of preservation, when observing, when starting to decay, takes out the exosper of seed seed, red exosper is removed in stranding, remaining milky seed, dries moisture, be positioned over 0 ~ 4 ℃ standby;
(2) by Gibberellins solution, process seed: under the constant incubator and low light condition of 18 ~ 22 ℃, first use distilled water immersion seed 24 ~ 48h; By the Gibberellins solution that concentration is 200 mg/L~500 mg/L, soak milky seed again, outwell Gibberellins solution after soaking 24 ~ 48h, water rinses the seed after soaking, until clean residual gibberellin;
(3) under aseptic condition, be seed sterilizing: in superclean bench, first use aseptic washing seed 3 ~ 5 times, then service property (quality) percentage concentration 70 ~ 80% alcohol disinfecting 30 ~ 45s, aseptic washing 3 ~ 5 times, re-use mass percentage concentration 0.1 ~ 0.15% mercuric chloride and soak 10 ~ 15 minutes, aseptic washing 3 ~ 5 times.
(4) wet husky alternating temperature stratification is processed: by sterilizing completely seed bury in wet sand, by burying seed in wet sand, be placed in turn under 0 ~ 4 ℃, 18 ~ 22 ℃, 0 ~ 4 ℃, 18 ~ 22 ℃ conditions and respectively cultivate respectively 45 ~ 60 days, wetly husky alternating temperature stratification total time reach 180 ~ 240 days;
(5) sprout and breed: the seed after directly wet husky alternating temperature stratification being processed makes its sprouting breed in soil, or the seed after wet husky alternating temperature stratification is processed is stayed in wet sand, under 18 ~ 22 ℃ of conditions, continue constant temperature culture 90 ~ 120 days, the radicle lignification of seed develops into main root, then the seed of sprouting is planted to field and breeds.
2. the long-pending method that paris polyphylla seed quick-speed germination is bred of gibberellin according to claim 1 and wet sand bed, is characterized in that: in step (2), by Gibberellins solution, process seed before first with distilled water seed soaking 24 ~ 48h; (3) seed sterilizing; (4) wet sand bed is long-pending 180 ~ 240 days.
CN201310342231.6A 2013-08-06 2013-08-06 Gibberellin and wet sand bed amass the method that paris polyphylla seed quick-speed germination is bred Expired - Fee Related CN103609223B (en)

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Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104206148A (en) * 2014-08-22 2014-12-17 迪庆州迪姆生态高新产业股份有限公司 Alternate cooling and heating paris polyphylla seed propagation method
CN104380942A (en) * 2014-09-27 2015-03-04 王伟 Processing method allowing seeds of Paris polyphylla var. yunnanensis to grow roots in four months and to grow leaves in eight months
CN105210647A (en) * 2015-10-28 2016-01-06 云南祥仁生物科技有限公司 The method for massively culturing of a kind of Yunnan Paris polyphylla seedling
CN105325094A (en) * 2015-11-26 2016-02-17 四川佳凯科技开发有限公司 Method for releasing dormancy of paris polyphylla seeds
CN105746129A (en) * 2016-03-03 2016-07-13 湖南利诺生物药业有限公司 Rapid propagation method for polyphylla seeds
CN106105481A (en) * 2016-07-01 2016-11-16 文山苗乡三七科技有限公司 A kind of germination accelerating method of Rhizoma Paridis seed
CN106416655A (en) * 2016-08-02 2017-02-22 景谷雄剑重楼种植专业合作社 Rhizoma Paridis artificial seedling growing method
CN106560017A (en) * 2016-08-04 2017-04-12 丽江十邦生物工程有限责任公司 Plantation method improving Rhizoma Paridis budding rate
CN106612713A (en) * 2016-11-07 2017-05-10 广西壮族自治区农业科学院花卉研究所 Method for promoting aseptic budding of Chinese rose seeds
CN106817952A (en) * 2016-12-23 2017-06-13 广西壮族自治区药用植物园 The method for promoting Paris polyphylla germination
CN107318303A (en) * 2017-08-14 2017-11-07 中南民族大学 A kind of method for promoting Paris polyphylla seed quick-speed germination seedling
CN107318624A (en) * 2017-08-17 2017-11-07 金秀圣康生态农业开发有限公司 A kind of paris polyphylla seed seedling-raising method
CN107371720A (en) * 2017-08-17 2017-11-24 金秀圣康生态农业开发有限公司 A kind of paris polyphylla efficient cultivation method
CN108650926A (en) * 2017-03-30 2018-10-16 浙江中医药大学 Method of the exogeneous growth conditioning agent to abolishing magnificent Paris polyphylla seed dormancy
CN109169016A (en) * 2018-09-25 2019-01-11 福建省农业科学院农业生物资源研究所 A kind of mating system that paris polyphylla seed quickly comes into leaves
CN109220062A (en) * 2018-11-26 2019-01-18 辽宁省经济作物研究所 A method of promoting Smilacina japonica germination
CN109526652A (en) * 2018-12-28 2019-03-29 天全县远航农业有限公司 A kind of novel Paris polyphylla high efficiency seedling cultivating method based on low-temperature treatment and enzymatic treatment
CN110140472A (en) * 2019-05-23 2019-08-20 中国林业科学研究院亚热带林业实验中心 A method of promote paris polyphylla seed to sprout

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Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104206148A (en) * 2014-08-22 2014-12-17 迪庆州迪姆生态高新产业股份有限公司 Alternate cooling and heating paris polyphylla seed propagation method
CN104380942A (en) * 2014-09-27 2015-03-04 王伟 Processing method allowing seeds of Paris polyphylla var. yunnanensis to grow roots in four months and to grow leaves in eight months
CN105210647B (en) * 2015-10-28 2017-12-26 云南祥仁生物科技有限公司 A kind of method for massively culturing of Yunnan Paris polyphylla seedling
CN105210647A (en) * 2015-10-28 2016-01-06 云南祥仁生物科技有限公司 The method for massively culturing of a kind of Yunnan Paris polyphylla seedling
CN105325094A (en) * 2015-11-26 2016-02-17 四川佳凯科技开发有限公司 Method for releasing dormancy of paris polyphylla seeds
CN105746129A (en) * 2016-03-03 2016-07-13 湖南利诺生物药业有限公司 Rapid propagation method for polyphylla seeds
CN105746129B (en) * 2016-03-03 2018-06-29 湖南利诺生物药业有限公司 Paris polyphylla seed fast breeding method
CN106105481A (en) * 2016-07-01 2016-11-16 文山苗乡三七科技有限公司 A kind of germination accelerating method of Rhizoma Paridis seed
CN106416655A (en) * 2016-08-02 2017-02-22 景谷雄剑重楼种植专业合作社 Rhizoma Paridis artificial seedling growing method
CN106560017A (en) * 2016-08-04 2017-04-12 丽江十邦生物工程有限责任公司 Plantation method improving Rhizoma Paridis budding rate
CN106612713A (en) * 2016-11-07 2017-05-10 广西壮族自治区农业科学院花卉研究所 Method for promoting aseptic budding of Chinese rose seeds
CN106817952A (en) * 2016-12-23 2017-06-13 广西壮族自治区药用植物园 The method for promoting Paris polyphylla germination
CN108650926A (en) * 2017-03-30 2018-10-16 浙江中医药大学 Method of the exogeneous growth conditioning agent to abolishing magnificent Paris polyphylla seed dormancy
CN107318303A (en) * 2017-08-14 2017-11-07 中南民族大学 A kind of method for promoting Paris polyphylla seed quick-speed germination seedling
CN107371720A (en) * 2017-08-17 2017-11-24 金秀圣康生态农业开发有限公司 A kind of paris polyphylla efficient cultivation method
CN107318624A (en) * 2017-08-17 2017-11-07 金秀圣康生态农业开发有限公司 A kind of paris polyphylla seed seedling-raising method
CN109169016A (en) * 2018-09-25 2019-01-11 福建省农业科学院农业生物资源研究所 A kind of mating system that paris polyphylla seed quickly comes into leaves
CN109220062A (en) * 2018-11-26 2019-01-18 辽宁省经济作物研究所 A method of promoting Smilacina japonica germination
CN109526652A (en) * 2018-12-28 2019-03-29 天全县远航农业有限公司 A kind of novel Paris polyphylla high efficiency seedling cultivating method based on low-temperature treatment and enzymatic treatment
CN110140472A (en) * 2019-05-23 2019-08-20 中国林业科学研究院亚热带林业实验中心 A method of promote paris polyphylla seed to sprout
CN110140472B (en) * 2019-05-23 2021-03-23 中国林业科学研究院亚热带林业实验中心 Method for promoting germination of paris polyphylla seeds

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