CN101049060A - Breaking sleep and budding of notopterygium seeds - Google Patents
Breaking sleep and budding of notopterygium seeds Download PDFInfo
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- CN101049060A CN101049060A CN 200710049022 CN200710049022A CN101049060A CN 101049060 A CN101049060 A CN 101049060A CN 200710049022 CN200710049022 CN 200710049022 CN 200710049022 A CN200710049022 A CN 200710049022A CN 101049060 A CN101049060 A CN 101049060A
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- 230000034303 cell budding Effects 0.000 title 1
- 230000005059 dormancy Effects 0.000 claims abstract description 48
- 230000035784 germination Effects 0.000 claims abstract description 46
- 238000000034 method Methods 0.000 claims abstract description 32
- 238000009331 sowing Methods 0.000 claims abstract description 19
- 238000003475 lamination Methods 0.000 claims description 48
- 210000001161 mammalian embryo Anatomy 0.000 claims description 30
- 238000013517 stratification Methods 0.000 claims description 21
- 239000004576 sand Substances 0.000 claims description 17
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- 241000382951 Hansenia forbesii Species 0.000 claims description 9
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- 229920006395 saturated elastomer Polymers 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 6
- 241000208173 Apiaceae Species 0.000 claims description 3
- 241000533388 Hansenia weberbaueriana Species 0.000 claims description 3
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- 239000001963 growth medium Substances 0.000 abstract 1
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- 230000002618 waking effect Effects 0.000 abstract 1
- 238000011010 flushing procedure Methods 0.000 description 6
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- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
A dormancy waking up and germinating method for the seeds of notopterygium includes properly changing the temp to wake up said seeds in dormanant state, promoting the germination of said seeds, and controlling the temp, culture medium and sowing depth to obtain high-quality seedlings.
Description
Technical field
The present invention relates to the releasing of a kind of high crude drug seed dormancy phase and impel the method for seed sprouting, particularly, the present invention relates to break the notopterygium root seed dormancy and promote the method that the notopterygium root seed is sprouted, germinateed and emerges.
Background technology
Notopterygium root Notopterygium incisum Ting ex H.T.Chang is the herbaceos perennial of the distinctive Umbelliferae Notopterygium of China, be all the basic source plant of medicinal material notopterygium root with the notopterygium forbesii N.forbesii Boiss. that belongs to together, be used as medicine with rhizome and root, be one of medicinal herbs most in use of hiding in the tradition Qiang's medicine, mainly be distributed in the high and cold mountain area that economize in Sichuan, Qinghai, Gansu, Tibet etc., always rely on wild excavating.Predatoriness is in recent years excavated and habitat destruction, causes the producing region, various places to face the threat of resource exhaustion and seed resource forfeiture, has been put into national III level protective plant, and enters the red register of Chinese endangered species.Therefore, the artificial planting by scale is protection wild resource and the fundamental way that solves disparities between supply and demand.
Notopterygium root season oneself to cold, formed strong stress resistance in the plateau particular surroundings such as intensive ultraviolet radiation, hypoxemia, atmospheric depression, temperature drastic change, vegetative period is short, year increment is little, embryo hypoplasia, long and natural germination rate unicellular lower eukaryote of seed dormancy phase characteristic.The notopterygium root seed has after-ripening of embryo form and the dual hibernation feature of physiology after-ripening, and resting stage reaches for 8～October, brings very big difficulty to artificial domesticating cultivation.Lamination method (stratification) is the most frequently used seed dormant method of releasing, to breaking seed dormancy comprehensive effect is arranged, and it can make the kind embryo after-ripening of dormancy, makes the saturating property improvement of kind of skin, makes mortifier level reduction etc.; And method is simple, is convenient to operation, and cost is also lower, is fit to the application in the actual production.The lamination method needing to be particularly useful for form after-ripening and physiology after-ripening, the seed that dormancy time is long, or hard drupe class seed, and the seed and the multifactor seed that causes dormancy that contain germination inhicbitor matter.Under (referring generally to 1～10 ℃) in warm conditions or in the low temperature lamination, the seed that lamination is handled just can germinate.But different plants are different because of hibernation feature, and the lamination Temperature Treatment that need take also has nothing in common with each other.
By retrieval, we have only found the relevant report that utilizes plant hormone to handle the notopterygium forbesii seed, but it exists problems such as germination rate is low, unstable result.By the end of at present, do not see the stable report of high germination rate success that obtains of relevant notopterygium root seed treatment yet.
Summary of the invention
The object of the present invention is to provide and a kind ofly can effectively break the notopterygium root seed dormancy and promote the method that seed sprouting emerges, this method is hidden lamination and is handled by the notopterygium root plant seed being carried out sand, thereby the notopterygium root seed germination rate is stably brought up to more than 60%.
For achieving the above object, the technical solution used in the present invention is as follows:
The broken dormancy and the germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
The notopterygium root seed that collects is removed impurity and flat grain, require physical purity of seed to be not less than 95%, thousand kernel weight is not less than 3.1g, and seed vigor is not less than 75%;
B, seed soaking
To soak seed 12～24 hours to saturated with 10～40 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier river sand is cleaned up, naturally cool to room temperature after 2 hours in 130 ℃ of oven dry, again will be through the notopterygium root seed after step B handles and river sand by 1: 1～3 ratio and even, keeping humidity is 50～80%, then through stage alternating temperature stratification processing releasing seed dormancy; Detect germination rate and germination vigor.
Described stage alternating temperature stratification was undertaken by following three stages:
Lamination is 60～90 days under phase I 10～30 ℃ of conditions, and the embryo rate reaches 10～30%, changes next stage over to;
Lamination is 60～105 days under 6～15 ℃ of conditions of second stage, and the embryo rate reaches 70～90%, changes next stage over to;
Continued lamination 30～155 days under phase III 0～5 ℃ of condition, breaking dormancy.
Preferably:
Lamination is 60～80 days under phase I 15～25 ℃ of conditions, and the embryo rate reaches 10～20%, changes next stage over to;
Lamination is 60～90 days under 7～14 ℃ of conditions of second stage, and the embryo rate reaches 70～90%, changes next stage over to;
Continued lamination 60～90 days under phase III 0～5 ℃ of condition, breaking dormancy.
D, sowing
Will be in short texture, nutrient component is abundant, the content of organic matter is high germination matrix through the notopterygium root planting seed of outstanding kind of the skin of breaking dormancy, radicle, sowing depth 1～1.5cm, germination temperature remain on 10～25 ℃, grow seedling.Wherein,
Preferably: sowing depth 1.5cm, germination temperature remain on 10～15 ℃.
The notopterygium root seed of described steps A is to gather then, or collecting time do not surpass 4 years and be stored in 0～5 ℃ of dry environment under notopterygium root or notopterygium forbesii seed.
Described seed vigor is meant potential ability of seed sprouting or the vitality that embryo has.Adopt 0.1% tetrazole (TTC) method mensuration notopterygium root seed vigor.Method is as follows: 1. preliminary treatment: 6h prewets under 20 ℃ in the running water.2. sample is prepared: to surveying rip cutting, expose embryo and endosperm along seed back central authorities embryo.3.TTC dyeing: take by weighing a certain amount of red tetrazolium and be dissolved in the phosphate buffer (pH=7), be made into concentration and be 0.1% TTC solution.With the 11h that dyes under 30 ℃ of conditions of TTC solution.4. evaluation pre-treatment: TTC solution is rinsed well with running water.5. standard of perfection: embryo portion and endosperm all dye, and embryo portion all dyeing and endosperm dyes to the vitality seed is arranged greater than 4/5.
Described embryo rate=embryo length/endosperm long * 100%.
Described germination rate is meant the notopterygium root seed in suitable germination environment, and chitting piece accounts for the percentage of whole vernalization seed grain numbers; Described germination vigor is meant the notopterygium root seed in suitable germination environment, during by the 7th day chitting piece account for the percentage of whole vernalization seed grain numbers.Detect with filter paper method: the notopterygium root seed of breaking dormancy is sowed in the culture dish that is placed with double-deck filter paper, and filter paper soaks into running water, and keeping the photoperiod is 12/12 little time/dark reaction, keeps 10～25 ℃ temperature.
The germination matrix of described step D is the mixture that equivalent sawdust, vermiculite, peat composed of rotten mosses ash and perlite are formed.
The basic source plant of described notopterygium root seed is Umbelliferae herbaceos perennial notopterygium root Notopterygiumincisum Ting ex H.T.Chang and notopterygium forbesii N.forbesii Boissieu.
The invention has the advantages that:
1, the present invention is by employing stage alternating temperature stratification method, effectively removed the notopterygium root Seed Dormancy, reach more than 60% through the notopterygium root percentage of seedgermination of 220 days left and right sides breaking dormancies of the inventive method is stable, and reach as high as more than 90%, the proper old seed of store method also can reach higher germination rate, far above 0.52% germination rate under the natural conditions, also be higher than existing bibliographical information less than 20% germination rate.
2, by regulation and control to conditions such as temperature, medium, thickness of earth-fill cover in the notopterygium root seed germination process, effectively improved germinating capacity, emergence rate and the planting percent of seed, obtained high-quality seedling.With regard to the notopterygium root seed of breaking dormancy, percentage of seedgermination reaches 61～77%, germination vigor 36～69%, the cotyledon rate 83～89% of being unearthed, and planting percent reaches 56～80%.
3, the present invention need not any chemical HORMONE TREATMENT, ecological, environmental protective, and method is simple, is convenient to operation, and cost is also lower, is suitable for production application.Both can reduce the excavating of wild notopterygium root, the stock number of fast quick-recovery plateau wild stocks, but also already provide stable, abundant medicine source and technical guarantee for middle Tibetan medicine.
Embodiment
Embodiment one
A kind of broken dormancy and germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
Selection ripe then, the fruit that gather in September, 2003 from the Ganzi County, Sichuan do not ftracture as yet, and the wild notopterygium root seed of pericarp green is removed impurity and flat grain, and physical purity of seed is 95.5%, thousand kernel weight 3.49g, vitality 91.50%;
B, seed soaking
To soak seed 12 hours to saturated with 20 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier soil is removed in the river sand flushing, naturally cools to room temperature after 2 hours in 130 ℃ of oven dry, again will through the notopterygium root seed after step B handles and river sand by 1: 3 ratio with spare, carry out the stage alternating temperature stratification then:
Phase I: lamination is 50 days under 22 ℃ of conditions, and the embryo rate reaches 17.38%, changes next stage over to;
Second stage: lamination is 80 days under 10 ℃ of conditions, and the embryo rate reaches 80.23%, changes next stage over to;
Phase III: lamination is 64～104 days under 0～5 ℃ of condition, breaking dormancy.
Lamination to the 224 days, detecting germination rate is (74.66 ± 2.09) %, germination vigor (53.87 ± 1.87) %; Lamination to the is in the time of 234 days, and detecting germination rate is (85.46 ± 1.53) %, germination vigor (82.06 ± 1.13) %.
D, sowing
Will be in the matrix of forming by sawdust, vermiculite, peat composed of rotten mosses ash and perlite mixed in equal amounts through breaking dormancy, the outstanding notopterygium root planting seed of skin of planting of radicle, sowing depth 1.5cm, temperature remains on 15 ℃, grow the notopterygium root seedling, observation obtains, cotyledon rate (89.33 ± 4.16) % that is unearthed, planting percent (80.33 ± 3.79) %, cotyledon is unearthed 10 days phases, 16 days seeding stages.
Embodiment two
A kind of broken dormancy and germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
Selection ripe then, the fruit that gather in September, 2003 from the Ganzi County, Sichuan do not ftracture as yet, and the wild notopterygium root seed of pericarp green is removed impurity and flat grain, and physical purity of seed is 95.5%, thousand kernel weight 3.49g, vitality 91.50%;
B, seed soaking
To soak seed 12 hours to saturated with 20 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier soil is removed in the river sand flushing, naturally cools to room temperature after 2 hours in 130 ℃ of oven dry, again will through the notopterygium root seed after step B handles and river sand by 1: 2 ratio with spare, carry out the stage alternating temperature stratification then:
Phase I: lamination is 84 days under 20 ℃ of conditions, and the embryo rate reaches 20.86%, changes next stage over to;
Second stage: lamination is 80 days under 10 ℃ of conditions, and the embryo rate reaches 76.42%, changes next stage over to;
Phase III: lamination is 64～74 days under 0～5 ℃ of condition, breaking dormancy.
Lamination to the 224 days, detecting germination rate is (68.52 ± 2.31) %, germination vigor (53.33 ± 1.08) %; Lamination to the is in the time of 234 days, and detecting germination rate is (82.20 ± 1.53) %, germination vigor (77.04 ± 1.02) %.
D, sowing
Will be in the matrix of forming by sawdust, vermiculite, peat composed of rotten mosses ash and perlite mixed in equal amounts through breaking dormancy, the outstanding notopterygium root planting seed of skin of planting of radicle, sowing depth 1.0cm, temperature remains on 15 ℃, grow the notopterygium root seedling, observation obtains, cotyledon rate (87.67 ± 1.53) % that is unearthed, planting percent (77.33 ± 2.31) %, cotyledon is unearthed 8 days phases, 14 days seeding stages.
Embodiment three
A kind of broken dormancy and germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
Selection from Ganzi County, state, Ganzi, Sichuan Province in 2002 10 gather and in 0～5 ℃ of dry environment, preserve the ripe wild notopterygium root seed in 1 year, remove impurity and flat grain, physical purity of seed is 98%, thousand kernel weight 3.10g, vitality 76.75%;
B, seed soaking
To soak seed 12 hours to saturated with 30 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier soil is removed in the river sand flushing, naturally cools to room temperature after 2 hours in 130 ℃ of oven dry, again will through the notopterygium root seed after step B handles and river sand by 1: 2 ratio with spare, carry out the stage alternating temperature stratification then:
Phase I: lamination is 75 days under 15 ℃ of conditions, and the embryo rate reaches 19.46%, changes next stage over to;
Second stage: lamination is 103 days under 10 ℃ of conditions, and the embryo rate reaches 84.69%, changes next stage over to;
Phase III: lamination is 32～65 days under 4 ℃ of conditions, breaking dormancy.
Lamination to the is in the time of 210 days, and detecting germination rate is (58.67 ± 2.00) %, germination vigor (42.67 ± 1.21) %; Lamination to the is in the time of 243 days, and detecting germination rate is (70.91 ± 1.02) %, germination vigor (53.33 ± 1.05) %.
D, sowing
Will be in the matrix of forming by equivalent sawdust, vermiculite, peat composed of rotten mosses ash and perlite through the notopterygium root planting seed behind the breaking dormancy, sowing depth 1cm, temperature remains on 20 ℃, grows the notopterygium root seedling.
Embodiment four
A kind of broken dormancy and germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
From Rangtang County, Aba state, Sichuan Province in October, 2002 gather and in 0～5 ℃ of dry environment, preserve ripe wild notopterygium root seed, remove impurity and flat grain, physical purity of seed is 95%, thousand kernel weight 3.19g, vitality 93.00%;
B, seed soaking
To soak seed 15 hours to saturated with 25 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier soil is removed in the river sand flushing, naturally cools to room temperature after 2 hours in 130 ℃ of oven dry, again will through the notopterygium root seed after step B handles and river sand by 1: 2 ratio with spare, carry out the stage alternating temperature stratification then:
Phase I: lamination is 63 days under 20 ℃ of conditions, and the embryo rate reaches 19.57% and changes next stage over to;
Second stage: lamination is 60 days under 12 ℃ of conditions, and the embryo rate reaches 74.36%, changes next stage over to;
Phase III: lamination is 68～155 days under 4 ℃ of conditions, breaking dormancy.
Lamination to the is in the time of 268 days, and detecting germination rate is (74 ± 1.23) %, germination vigor (62.67 ± 2.34) %; Lamination to the is in the time of 278 days, and detecting germination rate is (92.03 ± 2.46) %, germination vigor (66.28 ± 3.1) %.
D, sowing
Will be in the matrix of forming by equivalent sawdust, vermiculite, peat composed of rotten mosses ash and perlite through breaking dormancy, the outstanding notopterygium root planting seed of planting skin of radicle, sowing depth 1.5cm, temperature remains on 25 ℃, grows the notopterygium root seedling.
Embodiment five
A kind of broken dormancy and germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
Ripe then, fruit that selection is gathered from Ganzi County, state, Ganzi, Sichuan Province in September, 2003 are about to cracking, the beige wild notopterygium root seed of pericarp, remove impurity and flat grain, and physical purity of seed is 96.2%, thousand kernel weight 3.3g, vitality 89.50%;
B, seed soaking
To soak seed 24 hours to saturated with 10 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier the river sand flushing is gone soil in 130 ℃ of oven dry room temperature cooling after 2 hours, again will through the notopterygium root seed after step B handles and river sand by 1: 3 ratio with spare, carry out the stage alternating temperature stratification then:
Phase I: lamination is 70 days under 15 ℃ of conditions, and the embryo rate reaches 17.8% and changes next stage over to;
Second stage: lamination is 80 days under 10 ℃ of conditions, and the embryo rate reaches 78.92%, changes next stage over to;
Phase III: lamination is 60～80 days under 0～5 ℃ of condition, breaking dormancy.
Lamination to the is in the time of 215 days, and detecting germination rate is (50.68 ± 0.58) %, germination vigor (36.00 ± 1.00) %; Lamination to the is in the time of 222 days, and detecting germination rate is (60.00 ± 2.00) %, germination vigor (50.00 ± 4.00) %; Lamination to the is in the time of 227 days, and detecting germination rate is (86.77 ± 2.87) %, germination vigor (61.24 ± 6.04) %.
D, sowing
Will be in the matrix of forming by equivalent sawdust, vermiculite, peat composed of rotten mosses ash and perlite through breaking dormancy, the outstanding notopterygium root planting seed of planting skin of radicle, sowing depth 1.5cm, temperature remains on 15 ℃, grows the notopterygium root seedling.
Embodiment six
A kind of broken dormancy and germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
Gather in August, 2004 from the Dege county in selection through the ripe then notopterygium forbesii seed of artificial domestication, removes impurity and flat grain, and physical purity of seed is 97.5%, thousand kernel weight 5.29g, vitality 96.50%;
B, seed soaking
To soak seed 20 hours with 15 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier soil is removed in the river sand flushing, places 130 ℃ of oven dry room temperature cooling after 2 hours, again will through the notopterygium root seed after step B handles and river sand by 1: 3 ratio with spare, carry out the stage alternating temperature stratification then:
Phase I: lamination is 70 days under 20 ℃ of conditions, and the embryo rate reaches 20.13%, changes next stage over to;
Second stage: lamination is 65 days under 13 ℃ of conditions, and the embryo rate reaches 56.08, changes next stage over to;
Phase III: lamination is 60～80 days under 4 ℃ of conditions, breaking dormancy.
Lamination to the is in the time of 195 days, and detecting germination rate is (72.67 ± 4.16) %.
D, sowing
Will be in the matrix of forming by equivalent sawdust, vermiculite, peat composed of rotten mosses ash and perlite through breaking dormancy, the outstanding notopterygium forbesii planting seed of planting skin of radicle, sowing depth 1.5cm, temperature remains on 20 ℃, grows the notopterygium forbesii seedling.
Claims (7)
1, the broken dormancy and the germinating method of notopterygium root seed is characterized in that comprising the following steps:
A, seed selection
With the notopterygium root seed that collects, remove impurity and flat grain, require physical purity of seed to be not less than 95%, thousand kernel weight is not less than 3.1g, and seed vigor is not less than 75%;
B, seed soaking
To soak seed 12～24 hours to saturated with 10～40 ℃ of clear water through the notopterygium root seed that steps A filters out;
C, husky Tibetan alternating temperature stratification and the broken dormancy of seed
Earlier river sand is cleaned up, naturally cool to room temperature after 2 hours in 130 ℃ of oven dry, again will be through the notopterygium root seed after step B handles and river sand by 1: 1～3 ratio and even, keeping humidity is 50～80%, then through stage alternating temperature stratification processing releasing seed dormancy;
D, sowing
Will be in short texture, nutrient component is abundant, the content of organic matter is high germination matrix through the notopterygium root planting seed of outstanding kind of the skin of breaking dormancy, radicle, sowing depth 1～1.5cm, germination temperature remain on 10～25 ℃, grow seedling.
2, the broken dormancy and the germinating method of notopterygium root seed as claimed in claim 1 is characterized in that stage alternating temperature stratification described in the step C was undertaken by following three stages:
Lamination is 60～90 days under phase I 10～30 ℃ of conditions, and the embryo rate reaches 10～30%, changes next stage over to;
Lamination is 60～105 days under 6～15 ℃ of conditions of second stage, and the embryo rate reaches 70～90%, changes next stage over to;
Continued lamination 30～155 days under phase III 0～5 ℃ of condition, breaking dormancy.
3, the broken dormancy and the germinating method of notopterygium root seed as claimed in claim 1 or 2 is characterized in that stage alternating temperature stratification described in the step C was undertaken by following three stages:
Lamination is 60～80 days under phase I 15～25 ℃ of conditions, and the embryo rate reaches 10～20%, changes next stage over to;
Lamination is 60～90 days under 7～14 ℃ of conditions of second stage, and the embryo rate reaches 70～90%, changes next stage over to;
Continued lamination 60～90 days under phase III 0～5 ℃ of condition, breaking dormancy.
4, the broken dormancy and the germinating method of notopterygium root seed as claimed in claim 1 is characterized in that the notopterygium root seed of described steps A is to gather then, or collecting time do not surpass 4 years and be stored in 0～5 ℃ of dry environment under notopterygium root or notopterygium forbesii seed.
5, the broken dormancy and the germinating method of notopterygium root seed as claimed in claim 1, the germination matrix that it is characterized in that described step D are the mixture that equivalent sawdust, vermiculite, peat composed of rotten mosses ash and perlite are formed.
6, the broken dormancy and the germinating method of notopterygium root seed as claimed in claim 1, the sowing depth that it is characterized in that described step D is 1.5cm, germination temperature remains on 10～15 ℃.
7, the broken dormancy and the germinating method of notopterygium root seed as claimed in claim 1 is characterized in that: the basic source plant of described notopterygium root seed is Umbelliferae herbaceos perennial notopterygium root Notopterygium incisum Ting exH.T.Chang and notopterygium forbesii N.forbesii Boissieu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100490227A CN101049060B (en) | 2007-04-30 | 2007-04-30 | Breaking sleep and budding of notopterygium seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100490227A CN101049060B (en) | 2007-04-30 | 2007-04-30 | Breaking sleep and budding of notopterygium seeds |
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| Publication Number | Publication Date |
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| CN101049060A true CN101049060A (en) | 2007-10-10 |
| CN101049060B CN101049060B (en) | 2011-03-30 |
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| CN2007100490227A Expired - Fee Related CN101049060B (en) | 2007-04-30 | 2007-04-30 | Breaking sleep and budding of notopterygium seeds |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416577B (en) * | 2008-11-27 | 2011-07-20 | 陇西中天药业有限责任公司 | Notopterygium forbesii seeds treatment and culture method |
| CN102599026A (en) * | 2012-03-30 | 2012-07-25 | 常熟市常福有机复合肥有限公司 | Tilia miqueliana seedling raising method |
| CN102612969A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Macrocarpium officinalis seedling raising method |
| CN102687613A (en) * | 2012-06-14 | 2012-09-26 | 西南大学 | Method for promoting orchardgrass seed germination |
| CN102948322A (en) * | 2012-11-19 | 2013-03-06 | 四川新荷花中药饮片股份有限公司 | Seed propagation method for quickly obtaining healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance |
| CN105684597A (en) * | 2016-01-28 | 2016-06-22 | 临洮农科教中药材开发有限公司 | Notopterygium root seed processing method and black mulching film land cultivating method of notopterygium roots |
| CN106069333A (en) * | 2016-06-25 | 2016-11-09 | 丽江翠森茂生物科技有限责任公司 | The processing method of a kind of Rhizoma Et Radix Notopterygii seed and seedling culture method |
| CN106171137A (en) * | 2016-08-09 | 2016-12-07 | 甘肃农业大学 | High-cold regions cold season upper soll layer lamination improves the method for caraway |
| CN106688358A (en) * | 2015-07-23 | 2017-05-24 | 中国中医科学院中药研究所 | Rapid germination of Chinese herb notopterygium forbesii seeds |
| CN107896553A (en) * | 2017-11-17 | 2018-04-13 | 融安广兴生态农业投资有限公司 | A kind of breeding method of climbing groundsel |
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2007
- 2007-04-30 CN CN2007100490227A patent/CN101049060B/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416577B (en) * | 2008-11-27 | 2011-07-20 | 陇西中天药业有限责任公司 | Notopterygium forbesii seeds treatment and culture method |
| CN102599026A (en) * | 2012-03-30 | 2012-07-25 | 常熟市常福有机复合肥有限公司 | Tilia miqueliana seedling raising method |
| CN102599026B (en) * | 2012-03-30 | 2013-06-26 | 常熟市常福有机复合肥有限公司 | Tilia miqueliana seedling raising method |
| CN102612969B (en) * | 2012-04-09 | 2013-04-17 | 常熟市海虹苗木花卉有限公司 | Macrocarpium officinalis seedling raising method |
| CN102612969A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Macrocarpium officinalis seedling raising method |
| CN102687613B (en) * | 2012-06-14 | 2013-06-05 | 西南大学 | Method for promoting orchardgrass seed germination |
| CN102687613A (en) * | 2012-06-14 | 2012-09-26 | 西南大学 | Method for promoting orchardgrass seed germination |
| CN102948322A (en) * | 2012-11-19 | 2013-03-06 | 四川新荷花中药饮片股份有限公司 | Seed propagation method for quickly obtaining healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance |
| CN102948322B (en) * | 2012-11-19 | 2014-04-16 | 四川新荷花中药饮片股份有限公司 | Seed propagation method for quickly obtaining healthy notopterygium incisum Ting ex H. T. Chang seedlings in abundance |
| CN106688358A (en) * | 2015-07-23 | 2017-05-24 | 中国中医科学院中药研究所 | Rapid germination of Chinese herb notopterygium forbesii seeds |
| CN105684597A (en) * | 2016-01-28 | 2016-06-22 | 临洮农科教中药材开发有限公司 | Notopterygium root seed processing method and black mulching film land cultivating method of notopterygium roots |
| CN106069333A (en) * | 2016-06-25 | 2016-11-09 | 丽江翠森茂生物科技有限责任公司 | The processing method of a kind of Rhizoma Et Radix Notopterygii seed and seedling culture method |
| CN106069333B (en) * | 2016-06-25 | 2019-09-06 | 丽江翠森茂生物科技有限责任公司 | A kind of processing method and seedling culture method of Rhizoma Et Radix Notopterygii seed |
| CN106171137A (en) * | 2016-08-09 | 2016-12-07 | 甘肃农业大学 | High-cold regions cold season upper soll layer lamination improves the method for caraway |
| CN107896553A (en) * | 2017-11-17 | 2018-04-13 | 融安广兴生态农业投资有限公司 | A kind of breeding method of climbing groundsel |
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