CN106069333A - The processing method of a kind of Rhizoma Et Radix Notopterygii seed and seedling culture method - Google Patents
The processing method of a kind of Rhizoma Et Radix Notopterygii seed and seedling culture method Download PDFInfo
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- CN106069333A CN106069333A CN201610474418.5A CN201610474418A CN106069333A CN 106069333 A CN106069333 A CN 106069333A CN 201610474418 A CN201610474418 A CN 201610474418A CN 106069333 A CN106069333 A CN 106069333A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
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Abstract
The present invention relates to a kind of processing method and the seedling culture method of Rhizoma Et Radix Notopterygii seed, described method for treating seeds includes: A1: pretreatment, obtains the first pretreatment seed；A2: the first lamination pretreatment stage；A3: the second lamination pretreatment stage；A4: the processing stage of final；Described seedling culture method includes: B1: implement the Rhizoma Et Radix Notopterygii seed treatment of above-mentioned steps A1 A4, obtains the Rhizoma Et Radix Notopterygii seed for directly sowing；B2: the plastics in seedling growth greenhouse are cultivated and are loaded compound material and mixed soil humous in bag, Rhizoma Et Radix Notopterygii seed points are sowed in described cultivation bag；B3: keeping the percent mass water content cultivating the mixed soil in bag is 30 50%, and to maintain the relative humidity of air be 70 80%, shade rate is 70 85%；B4: wait emerge neat after, just will cultivate bag integral transplanting to field, thus complete the cultivation of Rhizoma Et Radix Notopterygii seedling.Described method has good application potential and promotion prospect.
Description
Technical field
The present invention relates to processing method and the breeding method of this seedling of a kind of medical herb seed, more specifically, relate to
And the processing method of a kind of Rhizoma Et Radix Notopterygii seed and seedling culture method, belong to Seeds of Medicinal Plants and process and seedling culture field.
Background technology
Rhizoma Et Radix Notopterygii (Classification system: Notopterygium incisum Ting ex H.T.Chang), be a kind of Umbelliferae,
The medical herb of Notopterygium, is the distinctive rare medicinal herbs of China.Rhizoma Et Radix Notopterygii belongs to psychrophyte, China be mainly distributed on Sichuan,
, there are strict requirements in the High aititude region of the 2500-3700 rice on the ground such as Qinghai, Gansu and Tibet for growing environment and depend on
Rely.
Find through research, containing substantial amounts of pharmacy activity component, predominantly volatile oil, coumarin in the root of Rhizoma Et Radix Notopterygii, stem
The compound such as compounds, saccharide.A long time ago, China i.e. uses Rhizoma Et Radix Notopterygii to be used as medicine, be mainly used to treat flu, cold, headache,
Rheumatism, paralysis, joint ache, edema, various sores etc., be the famousst and precious a kind of Chinese medicine.
Having the most notable pharmaceutically active of Rhizoma Et Radix Notopterygii just, the most increasing to its demand, price goes up the most day by day.But with
This is corresponding, owing to the wild supply of Rhizoma Et Radix Notopterygii is the least, causes on the one hand its supply seriously to lag behind demand, it is impossible to
Meet normal demand；On the other hand, due to the rise of price, cause wild Rhizoma Et Radix Notopterygii resource to suffer excessive coyoting, close to exhausted,
Make Rhizoma Et Radix Notopterygii wild resource suffer serious, be difficult to the destruction recovered, make normal natural environment and wild resource excessively be broken
Bad, it is difficult to normally to recover.
Being based on artificial propagation, ethnic group plantation, protection wild resource etc. to consider, people are carried out for the breeding of Rhizoma Et Radix Notopterygii
Substantial amounts of research, achieves certain achievement.
But another aspect, Rhizoma Et Radix Notopterygii has that embryo form after-ripening is specific and physiology after-ripening characteristic, and this causes artificial propagation and training
Educate and there is great difficulty, there is many defects such as low, the poor repeatability of germination percentage.
In order to overcome these defects, people have carried out numerous studies for the process of Rhizoma Et Radix Notopterygii seed, and achieve certain
Achievement, such as:
CN101427680A discloses a kind of notopterygium incisium seed processing method, the steps include: first, prepares treatment fluid, processes
Formula of liquid is, by weight percentage: auxin 0.005-0.05%, gibberellins 0.01-0.1%, dehydrated alcohol 3%, and remaining is
Water；By the auxin weighed according to the above ratio, gibberellins mixing, with the anhydrous alcohol solution of aforementioned proportion, add water mixing；
Then weigh dry Rhizoma Et Radix Notopterygii seed, and take the treatment fluid of Rhizoma Et Radix Notopterygii grain weight 20%, the two is mixed thoroughly, locate under the conditions of 5-10 DEG C
Manage 190-210 days.
CN101049060A discloses broken dormancy and the germinating method of a kind of Rhizoma Et Radix Notopterygii seed, and it comprises the following steps: A, seed selection
The Rhizoma Et Radix Notopterygii seed that will collect, removes the removal of impurity and flat grain, it is desirable to physical purity of seed is not less than 95%, and mass of 1000 kernel is not less than 3.1g, plants
Sub-vitality is not less than 75%；B, seed soaking were by 10-40 DEG C of Seed soaking of Rhizoma Et Radix Notopterygii seed of filtering out through step A 12-24 hour
To saturated；River sand is first cleaned up by C, husky Tibetan alternating temperature stratification and broken dormancy of seed, naturally cools to after drying 2 hours in 130 DEG C
Room temperature, then by Rhizoma Et Radix Notopterygii seed and the river sand after step B processes in the ratio of 1:1-3 and even, holding humidity is 50-80%, so
Process by stage alternating temperature stratification and release seed dormancy；The Rhizoma Et Radix Notopterygii seed highlighting seed coat through breaking dormancy, radicle is broadcast by D, sowing
Kind in the Germination textures that short texture, rich in nutrition content, the content of organic matter are high, depth of planting 1-1.5cm, germination temperature is protected
Hold at 10-25 DEG C, grow seedling.
CN101416577A discloses seed treatment and the breeding method of a kind of Radix et Rhizoma Notopterygii, and the method includes that seed is adopted
The sport technique segments such as receipts, seed treatment, soil treatment, date of seeding, the amount of broadcasting, seedbed management.Use the process Radix et Rhizoma Notopterygii of the method
Emergence rate is more than 65%, and tradition directly nursery emergence rate, generally below 10%, is more than 6 times of tradition directly nursery.
CN102948322A discloses a kind of seed propagation method a large amount of, the quickly healthy and strong Rhizoma Et Radix Notopterygii seedling of acquisition, the first step
Carry out intermittent warming and promote that embryo is ripe；Second step, growth regulator processes, and releases seed dormancy, carries out accelerating germination；3rd step is right
The Rhizoma Et Radix Notopterygii seed of breaking dormancy carries out nutrition seed soaking, sowing, the bud that promotes to send out strong；After seed breaking dormancy, with the phosphorus of 0.8-1wt%
The mixed liquor seed soaking 2h of acid dihydride potassium and the micro-fertilizer of flying dragon board " flying dragon No. 1 " 0.2wt%, is then seeded in fertile soil content 60%
In seedbed, cover the fine earth of 1-2cm, wait germination and emergence.The method solve with Rhizoma Et Radix Notopterygii seed quickly, amount reproduction strong sprout
Method, has the advantage that repoductive time is fast, reproductive efficiency is high, seedling is healthy and strong, can solve artificial growth Rhizoma Et Radix Notopterygii well to seedling
Substantial amounts of demand.
CN104041221A discloses a kind of notopterygium incisium seed processing method, the method particularly sprouting pre-treatment, the method
Comprise the following steps: weigh 1000 grams of Rhizoma Et Radix Notopterygii seeds；With 50-5000ppm auxin by the 20% of Rhizoma Et Radix Notopterygii grain weight, admix Rhizoma Et Radix Notopterygii
Seed, mixes thoroughly, in 5-25 DEG C, after processing 90 days, by the 25% of Rhizoma Et Radix Notopterygii grain weight, admixes 50-500ppm gibberellins, mixes thoroughly,
Process 60 days under the conditions of 10-25 DEG C.Number takes 3 parts of Rhizoma Et Radix Notopterygii seed after process, 1000 every part, sprouts 1 week for 20 DEG C, and statistics is germinateed
Rate.After described method uses auxin and two kinds of plant treatment of gibberellins, Rhizoma Et Radix Notopterygii percentage of seedgermination can be significantly improved so that it is reach
More than 95%.The technical problem of key is solved for realizing the artificial standardized planting of Rhizoma Et Radix Notopterygii.
CN105284238A discloses the dormancy breaking method of a kind of Rhizoma Et Radix Notopterygii seed, and it specifically comprises the following steps that 1, Seeds preprocess:
Clean threshing Rhizoma Et Radix Notopterygii seed, remove the removal of impurity and flat grain, 25-30 DEG C of distilled water of preferred Rhizoma Et Radix Notopterygii seed is sealed seed soaking 5-10 little
Time；Take out, natural drying；2, husky Tibetan breaks dormancy: choosing smooth ground, desert, water pipe ending vertical is pointed to ground, water slug is husky
Get hole, Rhizoma Et Radix Notopterygii seed seed soaking completed is mixed homogeneously with substrate and is placed in hole, gently covers one layer of 1-1.5cm sandy soil,
The mode using drip irrigation to irrigate is carried out once for every two days, drips speed 3-5 and drips/s, and holding sand water content is 70-80%, husky Tibetan 60-90
My god, take out, filter, remove substrate, the Rhizoma Et Radix Notopterygii seed slept must be broken.
As it has been described above, prior art discloses the multiple method processing Rhizoma Et Radix Notopterygii seed, but for novel Qiang
Method for treating seeds alive, still suffers from continuing necessity and the demand of research, and this is for solving the artificial propagation of Rhizoma Et Radix Notopterygii and cultivating required
In a large amount of seedlings and nature, the contradiction of wild resource exhaustion problem has great importance, and this is also current Rhizoma Et Radix Notopterygii artificial culture
Study hotspot in technical field and emphasis, the power place that the present invention is accomplished especially and basis are leaned on.
Summary of the invention
In order to seek the new processing method of Rhizoma Et Radix Notopterygii seed and its seedling culture method, present inventor has performed substantial amounts of deeply
Enter research, after having paid creative work, thus complete the present invention.
The invention mainly relates to following aspects.
First aspect, the present invention relates to the processing method of a kind of Rhizoma Et Radix Notopterygii seed, the treating method comprises following steps:
A1: pretreatment, obtains the first pretreatment seed
Choose ripe, full Rhizoma Et Radix Notopterygii seed, successively soak 5-7 hour at a temperature of T1 with ammonia spirit, use deionization
Water soaks at normal temperatures 3-6 hour and soaks 8-12 hour at a temperature of T2 with dilute acid solution, obtains pretreatment seed；
A2: the first lamination pretreatment stage
Described pretreatment seed is pulled out, washs 2-3 time with clear water, and naturally dry to dry tack free, then stir with river sand
Mixing uniformly, cover 1cm thickness river sand, then lamination 40-50 days at 5-10 DEG C again in top, period keeps the quality hundred of river sand
Point water content is W1, completes the first lamination pretreatment；
A3: the second lamination pretreatment stage
Liter high-temperature, to 15-20 DEG C, continues lamination 30-35 days at such a temperature, and period still keeps the percent mass of river sand
Water content is W1, completes the second lamination pretreatment；
A4: the processing stage of final
After completing above-mentioned second lamination pretreatment, spray diluted alcohol aqueous solution in good time, aqueous with the percent mass keeping river sand
Amount is W2, after 8-12 days, terminates, completes finally to process, thus obtain the Rhizoma Et Radix Notopterygii seed being disposed.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A1, the Rhizoma Et Radix Notopterygii seed used, permissible
Select the seed that Rhizoma Et Radix Notopterygii raw for 4-5 is tied, row filter of going forward side by side, remove the seed of the existing defects such as shrivelled, damage, thus obtain
Ripe, full Rhizoma Et Radix Notopterygii seed.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A1, described ammonia spirit is according to NH3Meter,
Its mass percent concentration is 5-9%, such as, can be 5%, 6%, 7%, 8% or 9%.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, the acid in described step A1, in described dilute acid solution
For citric acid, acetic acid, propanoic acid, hydrochloric acid or sulphuric acid, most preferably citric acid.
Wherein, the mass percent concentration of described dilute acid solution is 4-8%, can be such as 4%, 5%, 6%, 7% or
8%.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A1, described T1 temperature is 10-14 DEG C,
Can be such as 10 DEG C, 11 DEG C, 12 DEG C, 13 DEG C or 14 DEG C, most preferably 12 DEG C.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A1, described T2 temperature is 18-24 DEG C,
Can be such as 18 DEG C, 20 DEG C, 22 DEG C or 24 DEG C.
Inventor finds, preferably T2 temperature and the difference of T1 temperature, i.e. T2-T1=7-9 DEG C, most preferably T2-T1=8 DEG C,
Now can obtain the most excellent technique effect.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A2, described river sand is in river
The tiny grains of sand, such as, can be to be 10% time in the aqueous percentage amounts of quality, and hands clenches and the pockets of grains of sand, and those skilled in the art can
Suitably selecting and determine, this is the conventional capability that should possess in this area, is no longer described in detail at this.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A2, described pretreatment seed with carry out
The mass ratio of the river sand (the most not including carrying out later the river sand thick for 1cm of top layer covering) of stirring is 1:2-3, can be such as 1:2,
1:2.5 or 1:3.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A2, the percent mass of described river sand contains
Water yield W1 is 50-60%, such as, can be 50%, 55% or 60%.This percent mass water content can be by timing detection river sand
Water content, if less than this W1, then carrying out uniform moisture spray, so that it is in the whole phase of the first lamination pretreatment stage
Between be held in the range of 50-60%.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A2, Stratification time is 40-50 days, example
As being 40 days, 45 days or 50 days.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A3, Stratification time is 30-35 days, and
All keep the percent mass water content of river sand still for W1 (i.e. 50-60%) during the second whole lamination pretreatment, but temperature by
5-10 DEG C of step A2 is increased to 15-20 DEG C.
The inventors discovered that, although the percent mass water content of river sand is constant, but by the rising of temperature, but can obtain more
For excellent technique effect.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, the alcohol in described step A4, in described diluted alcohol aqueous solution
For any one in methanol, ethanol, isopropanol, normal propyl alcohol, isobutanol, sec-butyl alcohol or n-butyl alcohol, most preferably n-butyl alcohol.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A4, the quality of described diluted alcohol aqueous solution
Percent concentration is 3-7%, such as, can be 3%, 4%, 5%, 6% or 7%, most preferably 5%.
In the described processing method of the Rhizoma Et Radix Notopterygii seed of the present invention, in described step A4, described percent mass water content is
65-80%, such as, can be 65%, 70%, 75% or 80%, most preferably 70%.
As it has been described above, the invention provides the processing method of a kind of Rhizoma Et Radix Notopterygii seed, by above-mentioned steps A1-A4 place
Reason, obtained Rhizoma Et Radix Notopterygii seed can carry out at once germinateing and cultivate, through being processed as (the limit of the most plurality of technical characteristic
Fixed and comprehensive synergism) the Rhizoma Et Radix Notopterygii seed that obtains, there is the effect of many excellences, such as excellent germination percentage, germinating energy etc.,
And the time of process is short, highly shortened the self heating time of seed, meets the urgent needs of implant mass.
Second aspect, the invention still further relates to the breeding method of a kind of Rhizoma Et Radix Notopterygii seedling, and described breeding method includes walking as follows
Rapid:
B1: implement the Rhizoma Et Radix Notopterygii seed treatment of above-mentioned steps A1-A4, thus obtain the Rhizoma Et Radix Notopterygii seed for directly sowing；
B2: the plastics in seedling growth greenhouse are cultivated and are loaded compound material in bag (can be such as degradable plastic bag) with humous
Mixed soil, is then sowed at Rhizoma Et Radix Notopterygii seed points in described cultivation bag；
B3: keeping the percent mass water content cultivating the mixed soil in bag is 30-50%, and maintains the wettest of air
Degree is 70-85% for 70-80%, shade rate；
B4: wait emerge neat after, just will cultivate bag integral transplanting to field, thus complete the cultivation of Rhizoma Et Radix Notopterygii seedling.
In the breeding method of the described Rhizoma Et Radix Notopterygii seedling of the present invention, in described step B2, described compound material and fertile soil
Mass ratio be 1:3-4, can be such as 1:3,1:3.5 or 1:4.
Wherein, described compound material in parts by weight, the concrete component including following:
In described compound material, the weight portion of described Modification of kaolin fertilizer slow release granule is 4-6 part, can be such as 4 parts,
5 parts or 6 parts.
Described Modification of kaolin fertilizer slow release granule is to obtain according to the preparation method comprised the steps:
C1: Kaolin is calcined 50-70 minute at 500-600 DEG C, is crushed to 50-100 mesh after natural cooling, is forged
Powder after burning；
C2: powder after described calcining is joined in the mixed aqueous solution of potassium nitrate, ammonium phosphate and ammonium chloride three, and
Energy density be 2-3Kw/L ultrasound condition under dispersed with stirring 10-20 minute, obtain dispersion soln；
C3: by dispersion soln under agitation, is evaporated in 50-60 DEG C, then carries out pelletize, and obtaining granularity is 1-
The granule of 1.5mm, dries completely, obtains described Modification of kaolin fertilizer slow release granule.
Wherein, in step C2, in described mixed aqueous solution, the mass percent of potassium nitrate, ammonium phosphate and ammonium chloride
Concentration is respectively 15-25%, 8-15% and 20-30%.
Wherein, in step C2, after described calcining, powder is 1:3-5 with the mass ratio of mixed aqueous solution, such as, can be 1:
3,1:4 or 1:5.
Wherein, in step C3, prilling process when carrying out pelletize is very known routine techniques means, this area skill
Art personnel can carry out suitably selected and determine (such as just can carry out pelletize when moisture is evaporated to be suitable for pelletize), at this not
It is described in detail again.
In described compound material, described plant fermentation become thoroughly decomposed material weight portion be 30-40 part, can be such as 30 parts, 35 parts
Or 40 parts.
The described plant fermentation material that becomes thoroughly decomposed is to obtain according to the preparation method comprised the steps:
D1: by 30 weight portion rice straw powder, 4 weight portion peanut hull meals, 6 weight portion Dry chick faeces, 2 weight portion Semen arachidis hypogaeaes
Cake, 2 weight portion drying bagasses, 1.5 parts by weight Corn powder, 1 weight portion bean cake, 0.5 parts sulfuric acid potassium, 15 weight parts waters enter
Row mixing, after mix homogeneously, is stacked in long 5 meters, the fermentation tank of wide 1.5 meters, and ulking thickness is 1 meter, obtains stockpile；
D2: 0.1 weight portion bacillus cereus and 0.05 weight portion yeast are put in 10 weight parts waters, after being uniformly dispersed,
Uniformly spray on stockpile；Then stockpile heats up naturally, and control centre's temperature, after 50-55 DEG C of bottom fermentation 2-3 days, is fully turned over
Material, obtains Preliminary fermentation material；
D3: join in 10 weight parts waters by 0.04 weight portion EM bacterium, after being uniformly dispersed, uniformly spray is to Preliminary fermentation material
On, after central temperature reaches 75 DEG C, maintain this temperature to fluctuate up and down less than 3 DEG C, and heap fermentation 30-35 hour, finally
Go out groove, dry, obtain described plant fermentation and become thoroughly decomposed material.
In described compound material, the weight portion of described floats is 5-10 part, such as, can be 5 parts, 7 parts, 9 parts or 10
Part.
Wherein, the granularity of described floats is 50-100 mesh.
In described compound material, the weight portion of described dry sawdust is 1-2 part, such as, can be 1 part, 1.5 parts or 2 parts.
The kind of this sawdust is not particularly limited, such as, can be the sawdust of multiple timber, or be wood-working factory
In the mixing wood shavings that obtains.
In described compound material, the weight portion of described trace element mixture is 0.06-0.1 part, can be such as 0.06 part,
0.08 part or 0.1 part.
Wherein, described trace element mixture is the mixing of the magnesium sulfate of mass ratio 1:1:0.5, zinc nitrate and sodium selenate
Thing.
In the breeding method of the described Rhizoma Et Radix Notopterygii seedling of the present invention, the preparation method of the described compound material in step B2 is such as
Under:
E1: weigh the Modification of kaolin fertilizer slow release granule of respective weight portion respectively, material, apatite are become thoroughly decomposed in plant fermentation
Powder, dry sawdust and trace element mixture；
E2: each component above-mentioned be thoroughly mixed, obtains described compound material.
In the breeding method of the described Rhizoma Et Radix Notopterygii seedling of the present invention, in step B3, keep cultivating the mixed soil in bag
Percent mass water content be 30-50%, can be such as 30%, 40% or 50%；The relative humidity maintaining air is 70-
80%, can be such as 70%, 75% or 80%；Protection degree is 70-85%, such as, can be 70%, 75%, 80% or 85%.
In the breeding method of the described Rhizoma Et Radix Notopterygii seedling of the present invention, after having implemented the outplanting of step B4, just can be by
Daily management is carried out according to normal Rhizoma Et Radix Notopterygii cultivation technique, until results.
As it has been described above, present invention also offers the breeding method of a kind of Rhizoma Et Radix Notopterygii seedling, described method is by using above-mentioned side
The Rhizoma Et Radix Notopterygii seed that method obtains, and use specific compound material, thus it is remarkably improved seedling percent and healthy and strong degree, after the transfer
Survival rate is high, well-grown, it is to avoid the generation of high death rate, it is possible to provide substantial amounts of children for normal Rhizoma Et Radix Notopterygii artificial culture
Seedling, has significant advantage in actual planting process, thus has a good application prospect, and solves current Rhizoma Et Radix Notopterygii seedling tight
The defect scarce, survival rate is low.
Detailed description of the invention
Below by concrete, process example/the present invention is described in detail for cultivation example, but these exemplary embodiments
Purposes and purpose be only used for enumerating the present invention, not the real protection scope of the present invention is constituted any type of any limit
Fixed, more non-protection scope of the present invention is confined to this.
The process of Rhizoma Et Radix Notopterygii seed
Process example 1:T1 and the investigation of T2 temperature
A1: pretreatment, obtains the first pretreatment seed
Choosing maturation, full Rhizoma Et Radix Notopterygii seed that Rhizoma Et Radix Notopterygii raw for 4-5 is tied, successively with ammonia spirit, (described ammonia is molten
Liquid is according to NH3Meter, its mass percent concentration is 7%) 12 DEG C soak 6 hours, soak at normal temperatures with deionized water 4.5 hours
Soak 10 hours with at a temperature of the aqueous citric acid solution that mass percent concentration is 6% in table 1 below shown T2, obtain pre-place
Reason seed；
A2: the first lamination pretreatment stage
Described pretreatment seed is pulled out, washs 2-3 time with clear water, and naturally dry to dry tack free, according still further to quality
Stirring with river sand than 1:2.5, cover 1cm thickness river sand, then lamination 45 days at 8 DEG C again in top, period keeps river
Husky percent mass water content is 55%, completes the first lamination pretreatment；
A3: the second lamination pretreatment stage
Liter high-temperature, to 18 DEG C, continues lamination 32 days at such a temperature, and period still keeps the percent mass water content of river sand
It is 55%, completes the second lamination pretreatment；
A4: the processing stage of final
After completing above-mentioned second lamination pretreatment, spray mass percent concentration is the n-butanol aqueous solution of 5% in good time, with
The percent mass water content keeping river sand is 70%, after 10 days, terminates, completes finally to process, thus obtain the Qiang being disposed
Seed alive.
Wherein, when temperature T2 in step A1 is the different temperatures in table 1 below, obtain the Rhizoma Et Radix Notopterygii seed that numbering is different,
Taking 500 the most respectively, germination percentage when using filter paper method to carry out the 5th day and germinating energy test, this filter paper method is seed germination
The most popular method of test, (double-layer filter paper, is positioned in culture dish, and distilled water infiltration is protected to belong to very known technological means
Wet, temperature is 20 DEG C), concrete outcome see table 1.
Table 1
As can be seen here, in the notopterygium incisium seed processing method of the present invention, when the difference of T2-T1 is 8 DEG C, it is possible to obtain
High germination percentage and best germinating energy, and when deviation 8 DEG C is the biggest, then germination percentage and germinating energy are all significantly reduced.
In order to proceed deep investigation, inventor has carried out the investigation of all combinations to temperature T1 and T2 simultaneously, needs
It should be noted that in addition to temperature T1 and T2, other operation the most constant (being i.e. entirely identical to above step A1-A4), specifically
Result see table 2.
Table 2
From upper table 2 and the data that combine table 1, for T2-T1, even if having identical difference, but work as
When T1 is 12 DEG C, it is possible to obtain best technique effect while being other temperature (be better than T1).And for any temperature T1 and
Speech, when T2-T1=8 DEG C, no matter what temperature value T1 takes, be effect when 8 DEG C be all better than other T2-T1 difference time effect
Really.
Thus demonstrate: 1, T1 is most preferably 12 DEG C；2, T2-T1 is most preferably 8 DEG C.The two condition is met when simultaneously
Time, it is possible to obtain the most excellent germination percentage and germinating energy, also demonstrate both process temperature can significantly, unpredictable
Affect the sprouting performance of Rhizoma Et Radix Notopterygii seed.
Process example 2: the investigation of acid
A1: pretreatment, obtains the first pretreatment seed
Choosing maturation, full Rhizoma Et Radix Notopterygii seed that Rhizoma Et Radix Notopterygii raw for 4-5 is tied, successively with ammonia spirit, (described ammonia is molten
Liquid is according to NH3Meter, its mass percent concentration is 7%) 12 DEG C soak 6 hours, soak at normal temperatures with deionized water 4.5 hours
Soak 10 hours at 20 DEG C with aqueous acids different in the table 3 below that mass percent concentration is 6%, obtain pretreatment kind
Son；
A2: the first lamination pretreatment stage
Described pretreatment seed is pulled out, washs 2-3 time with clear water, and naturally dry to dry tack free, according still further to quality
Stirring with river sand than 1:2.5, cover 1cm thickness river sand, then lamination 45 days at 8 DEG C again in top, period keeps river
Husky percent mass water content is 55%, completes the first lamination pretreatment；
A3: the second lamination pretreatment stage
Liter high-temperature, to 18 DEG C, continues lamination 32 days at such a temperature, and period still keeps the percent mass water content of river sand
It is 55%, completes the second lamination pretreatment；
A4: the processing stage of final
After completing above-mentioned second lamination pretreatment, spray mass percent concentration is the n-butanol aqueous solution of 5% in good time, with
The percent mass water content keeping river sand is 70%, after 10 days, terminates, completes finally to process, thus obtain the Qiang being disposed
Seed alive.
Carry out germination percentage and the germinating energy test of Rhizoma Et Radix Notopterygii seed according to the same procedure processing example 1, result see table 3.
Table 3
As can be seen here, even if under the double condition of most preferred temperature T1 and T2-T1, the kind of acid is similarly for
Whole germination index has significant impact, and citric acid can obtain best technique effect, other acid all cause germination percentage and
Germinating energy is significantly reduced, and the especially germinating energy of sulphuric acid has and the most significantly reduces.
The kind thus demonstrating acid selects there is uncertain impact for final Germination performance.
Process the investigation of example 3:W1 and W2
A1: pretreatment, obtains the first pretreatment seed
Choosing maturation, full Rhizoma Et Radix Notopterygii seed that Rhizoma Et Radix Notopterygii raw for 4-5 is tied, successively with ammonia spirit, (described ammonia is molten
Liquid is according to NH3Meter, its mass percent concentration is 7%) 12 DEG C soak 6 hours, soak at normal temperatures with deionized water 4.5 hours
Soak 10 hours at 20 DEG C with the aqueous citric acid solution that mass percent concentration is 6%, obtain pretreatment seed；
A2: the first lamination pretreatment stage
Described pretreatment seed is pulled out, washs 2-3 time with clear water, and naturally dry to dry tack free, according still further to quality
Stirring with river sand than 1:2.5, cover 1cm thickness river sand, then lamination 45 days at 8 DEG C again in top, period keeps river
Husky percent mass water content is 55%, completes the first lamination pretreatment；
A3: the second lamination pretreatment stage
Liter high-temperature, to 18 DEG C, continues lamination 32 days at such a temperature, and period still keeps the percent mass water content of river sand
It is 55%, completes the second lamination pretreatment；
A4: the processing stage of final
After completing above-mentioned second lamination pretreatment, spray mass percent concentration is the n-butanol aqueous solution of 5% in good time, with
The percent mass water content keeping river sand is the W2 numerical value shown in table 3 below, after 10 days, terminates, completes finally to process, thus obtain
The Rhizoma Et Radix Notopterygii seed being disposed.
Carry out germination percentage and the germinating energy test of Rhizoma Et Radix Notopterygii seed according to the same procedure processing example 1, result see table 4.
Table 4
Note: Z28 that " * " is referred to is actual is exactly Z3.
From upper table 4, during the numerical value of W2 selects, best technique effect can be obtained when for 70%.When for other
During numerical value, the sprouting performance of seed is all caused to decrease.
When the W1 in this process example is replaced with 50%, 60% respectively, repeat this process example, find its rule and
Variation tendency is identical with table 4, remains and can obtain best technique effect when W2 is 70%, specific as follows:
1, when W1 be 50%, W2 be 70% time, germination percentage and germinating energy are respectively 91.9% and 89.1%.
2, when W1 be 60%, W2 be 70% time, germination percentage and germinating energy are respectively 91.5% and 88.9%.
3, it is 50% (and being 60%) as W1, when W2 is respectively 65%, 68%, 72%, 75% and 80%, germination percentage and sending out
Bud gesture is 50% than W1 respectively, W2 reduces 2-8% and 2-10% when being 70%.
All these all demonstrates W2 and is most preferably 70%.
Process example 4: the investigation of alcohol
A1: pretreatment, obtains the first pretreatment seed
Choosing maturation, full Rhizoma Et Radix Notopterygii seed that Rhizoma Et Radix Notopterygii raw for 4-5 is tied, successively with ammonia spirit, (described ammonia is molten
Liquid is according to NH3Meter, its mass percent concentration is 7%) 12 DEG C soak 6 hours, soak at normal temperatures with deionized water 4.5 hours
Soak 10 hours at 20 DEG C with the aqueous citric acid solution that mass percent concentration is 6%, obtain pretreatment seed；
A2: the first lamination pretreatment stage
Described pretreatment seed is pulled out, washs 2-3 time with clear water, and naturally dry to dry tack free, according still further to quality
Stirring with river sand than 1:2.5, cover 1cm thickness river sand, then lamination 45 days at 8 DEG C again in top, period keeps river
Husky percent mass water content is 55%, completes the first lamination pretreatment；
A3: the second lamination pretreatment stage
Liter high-temperature, to 18 DEG C, continues lamination 32 days at such a temperature, and period still keeps the percent mass water content of river sand
It is 55%, completes the second lamination pretreatment；
A4: the processing stage of final
After completing above-mentioned second lamination pretreatment, spray different alcohol in the table 5 below that mass percent concentration is 5% in good time
Aqueous solution, to keep the percent mass water content of river sand for 70%, after 10 days, terminates, completes finally to process, thus processed
Complete Rhizoma Et Radix Notopterygii seed.
Carry out germination percentage and the germinating energy test of Rhizoma Et Radix Notopterygii seed according to the same procedure processing example 1, result see table 5.
Table 5
As can be seen here, even if under the multiple optimal conditions of most preferred temperature T1, T2-T1 and W1 and W2, alcohol
Kind has significant impact similarly for final germination index, and wherein n-butyl alcohol can obtain best technique effect, other
Alcohol all causes germination percentage to be significantly reduced with germinating energy (even if the isobutanol similar with n-butyl alcohol and sec-butyl alcohol), especially
It is that the germinating energy of sulphuric acid has and the most significantly reduces.
The kind thus demonstrating alcohol selects there is uncertain impact for final Germination performance.
The breeding method of Rhizoma Et Radix Notopterygii seedling
Preparation example 1: the preparation of Modification of kaolin fertilizer slow release granule
C1: Kaolin is calcined 60 minutes at 550 DEG C, is crushed to 50-100 mesh after natural cooling, powder after being calcined
End；
C2: powder after described calcining is joined the mixed aqueous solution (nitric acid of potassium nitrate, ammonium phosphate and ammonium chloride three
The mass percent concentration of potassium, ammonium phosphate and ammonium chloride is respectively 18%, 12% and 25%) in, and in energy density be
Under the ultrasound condition of 2.5Kw/L, dispersed with stirring 15 minutes, obtain dispersion soln；
C3: by dispersion soln under agitation, is evaporated in 55 DEG C, then carries out pelletize, and obtaining granularity is 1-1.5mm's
Granule, dries completely, obtains Modification of kaolin fertilizer slow release granule, is named outer GLT.
Contrast preparation example 1: the preparation of Modification of kaolin fertilizer slow release granule
(being the most only stirred disperseing 15 minutes) in addition to being omitted by the ultrasound condition in step C2, other operation is all
Constant, thus repeat to implement preparation example 1, the Modification of kaolin named GLT1 of fertilizer slow release granule that will obtain.
Contrast preparation example 2: the preparation of Modification of kaolin fertilizer slow release granule
In addition to the Kaolin in step C1 is not carried out calcining, (i.e. directly kaolin powder is broken to 50-100 mesh), other behaviour
Make the most constant, thus repeat to implement preparation example 1, the Modification of kaolin named GLT2 of fertilizer slow release granule that will obtain.
Preparation example 2: plant fermentation become thoroughly decomposed material preparation
D1: by 30 weight portion rice straw powder, 4 weight portion peanut hull meals, 6 weight portion Dry chick faeces, 2 weight portion Semen arachidis hypogaeaes
Cake, 2 weight portion drying bagasses, 1.5 parts by weight Corn powder, 1 weight portion bean cake, 0.5 parts sulfuric acid potassium, 15 weight parts waters enter
Row mixing, after mix homogeneously, is stacked in long 5 meters, the fermentation tank of wide 1.5 meters, and ulking thickness is 1 meter, obtains stockpile；
D2: 0.1 weight portion bacillus cereus and 0.05 weight portion yeast are put in 10 weight parts waters, after being uniformly dispersed,
Uniformly spray on stockpile；Then stockpile heats up naturally, and control centre's temperature, after 50-55 DEG C of bottom fermentation 2-3 days, is fully turned over
Material, obtains Preliminary fermentation material；
D3: join in 10 weight parts waters by 0.04 weight portion EM bacterium, after being uniformly dispersed, uniformly spray is to Preliminary fermentation material
On, after central temperature reaches 75 DEG C, maintain this temperature to fluctuate up and down less than 3 DEG C, and heap fermentation 33 hours, finally go out
Groove, dries, and obtains plant fermentation and becomes thoroughly decomposed material.
Compound material preparation example 1: the preparation of compound material
E1: weigh the GLT of 5 weight portions respectively, material, 8 weight portion phosphorus are become thoroughly decomposed in plant fermentation that 35 weight portion preparation examples 2 obtain
Limestone flour (granularity is 50-100 mesh), 1.5 weight portions be dried sawdust and 0.08 weight portion trace element mixture (for mass ratio 1:
The mixture of the magnesium sulfate of 1:0.5, zinc nitrate and sodium selenate)；
E2: each component above-mentioned be thoroughly mixed, obtains compound material, named FHL1.
Compound material preparation example 2: the preparation of compound material
In addition to the GLT in step E1 of above-mentioned compound material preparation example 1 is replaced with GLT1, other operation is the most constant, thus
Repeat to implement compound material preparation example 1, the named FHL2 of compound material that will obtain.
Compound material preparation example 3: the preparation of compound material
In addition to the GLT in step E1 of above-mentioned compound material preparation example 1 is replaced with GLT2, other operation is the most constant, thus
Repeat to implement compound material preparation example 1, the named FHL3 of compound material that will obtain.
Implement the breeding method of Rhizoma Et Radix Notopterygii seedling below, and investigate the impact for seedling percent etc. of the complex fertilizer produced by different material.
Cultivate example
B1: enforcement processes the notopterygium incisium seed processing method of step A1-A4 in example 1, thus obtains for directly sowing
Rhizoma Et Radix Notopterygii seed (specially implements to obtain the step A1-A4 method of seed Z3)；
B2: the plastics in seedling growth greenhouse are cultivated and are loaded compound material FHL1 and mixed soil humous (compound material in bag
FHL1 and mass ratio humous are 1:3.5), then Rhizoma Et Radix Notopterygii seed points is sowed in described cultivation bag；
B3: keeping the percent mass water content cultivating the mixed soil in bag is 40%, and maintains the relative humidity of air
Be 75%, shade rate be 75%；
B4: wait emerge neat after, just will cultivate bag integral transplanting to field, thus complete the cultivation of Rhizoma Et Radix Notopterygii seedling.
Comparative example 1
B1: enforcement processes the notopterygium incisium seed processing method of step A1-A4 in example 1, thus obtains for directly sowing
Rhizoma Et Radix Notopterygii seed (specially implements to obtain the step A1-A4 method of seed Z3)；
B2: the plastics in seedling growth greenhouse are cultivated and are loaded compound material FHL2 and mixed soil humous (compound material in bag
FHL2 and mass ratio humous are 1:3.5), then Rhizoma Et Radix Notopterygii seed points is sowed in described cultivation bag；
B3: keeping the percent mass water content cultivating the mixed soil in bag is 40%, and maintains the relative humidity of air
Be 75%, shade rate be 75%；
B4: wait emerge neat after, just will cultivate bag integral transplanting to field, thus complete the cultivation of Rhizoma Et Radix Notopterygii seedling.
Comparative example 2
B1: enforcement processes the notopterygium incisium seed processing method of step A1-A4 in example 1, thus obtains for directly sowing
Rhizoma Et Radix Notopterygii seed (specially implements to obtain the step A1-A4 method of seed Z3)；
B2: the plastics in seedling growth greenhouse are cultivated and are loaded compound material FHL3 and mixed soil humous (compound material in bag
FHL3 and mass ratio humous are 1:3.5), then Rhizoma Et Radix Notopterygii seed points is sowed in described cultivation bag；
B3: keeping the percent mass water content cultivating the mixed soil in bag is 40%, and maintains the relative humidity of air
Be 75%, shade rate be 75%；
B4: wait emerge neat after, just will cultivate bag integral transplanting to field, thus complete the cultivation of Rhizoma Et Radix Notopterygii seedling.
Comparative example 3
Except in step B2 only in addition to plastics are cultivated and are loaded fertile soil in bag, other operation is the most constant, thus repeats reality
Execute cultivation example, obtain comparative example 3.
In seedling growth greenhouse, starting from germinateing, record reaches the time needed for the emergence rate of 90%；And investigate be transplanted to field
Seedling rate alive when latter 1 year and growth apparent condition, result see table 6.
Table 6
Wherein, " √ √ √ " represents the most healthy and strong, and " √ √ " represents more healthy and strong, and " √ " represents that healthy and strong degree to be weaker than
" √ √ √ " and " √ √ ".
As can be seen here, in the Rhizoma Et Radix Notopterygii seedling culture method of the present invention, by specific compound material, (have its source in compound material
In the specific preparation method of concrete component) use, the technique effect of excellence can be obtained, be in particular in: 1, the present invention
Cultivating example and reach the shortest time of 90% emergence rate, the second rate alive after 1 year is the highest, and outward appearance growth conditions is good；2 and when multiple
The Kaolin of the Modification of kaolin fertilizer slow release granule in batch mixing does not carries out the ultrasonic disperse of particular energy density and (should be to maintain
The long-time of fertilizer controls release and uniformly release, thus can provide stable source of nutrition in a long time) and specifically
During calcination processing (enhancing the absorption to fertilizer component and release), all cause 90% emergence rate required time, Seedling rate of living after 1 year
(this demonstrate that the unobviousness of particular procedure means and the unpredictability of effect) has been deteriorated with growth conditions；3 and
When not using the compound material of the present invention, then each index worst (see comparative example 3).
In sum, the invention provides processing method and Rhizoma Et Radix Notopterygii seedling culture method, the described kind of a kind of Rhizoma Et Radix Notopterygii seed
Sub-processing method is by the integrated use of specific technological means and selection, thus can significantly improve its Germination performance.And in Qiang
Live in the breeding method of seedling, be further advanced by the specific compound material of use, be substantially shorter the neat Seedling time, and have excellence
1 year after live Seedling rate and outward appearance growth conditions.Thus, the method for treating seeds of the present invention and seedling culture method are the people of Rhizoma Et Radix Notopterygii
Work cultivation technique provides the seed and seedling that breeding is rapid, survival rate is high, growth conditions is good, has significantly application latent
Power.
Should be appreciated that the purposes of these embodiments is merely to illustrate the present invention and is not intended to limit the protection model of the present invention
Enclose.Additionally, it will also be appreciated that after the technology contents having read the present invention, the present invention can be made respectively by those skilled in the art
Planting change, amendment and/or modification, all these equivalent form of value falls within the guarantor that the application appended claims is limited equally
Within the scope of protecting.
Claims (10)
1. a processing method for Rhizoma Et Radix Notopterygii seed, the treating method comprises following steps:
A1: pretreatment, obtains the first pretreatment seed
Choose ripe, full Rhizoma Et Radix Notopterygii seed, successively at a temperature of T1, soak 5-7 hour with ammonia spirit, exist with deionized water
Soak 3-6 hour under room temperature and soak 8-12 hour at a temperature of T2 with dilute acid solution, obtaining pretreatment seed；
A2: the first lamination pretreatment stage
Described pretreatment seed is pulled out, washs 2-3 time with clear water, and naturally dry to dry tack free, then with river sand stirring all
Even, cover 1cm thickness river sand, then lamination 40-50 days at 5-10 DEG C again in top, period keeps the percent mass of river sand to contain
The water yield is W1, completes the first lamination pretreatment；
A3: the second lamination pretreatment stage
Rising high-temperature to 15-20 DEG C, continue lamination 30-35 days at such a temperature, period still keeps the percent mass of river sand aqueous
Amount is W1, completes the second lamination pretreatment；
A4: the processing stage of final
After completing above-mentioned second lamination pretreatment, spray diluted alcohol aqueous solution, to keep the percent mass water content of river sand to be in good time
After W2,8-12 days, terminate, complete finally to process, thus obtain the Rhizoma Et Radix Notopterygii seed being disposed.
2. processing method as claimed in claim 1, it is characterised in that: the acid in described step A1, in described dilute acid solution
For citric acid, acetic acid, propanoic acid, hydrochloric acid or sulphuric acid, most preferably citric acid.
3. processing method as claimed in claim 1 or 2, it is characterised in that: in described step A1, described T1 temperature is 10-14
DEG C, described T2 temperature is 18-24 DEG C, and preferably T2-T1=7-9 DEG C, most preferably T2-T1=8 DEG C.
4. the processing method as described in any one of claim 1-3, it is characterised in that: in described step A2, the matter of described river sand
Amount single-detector W1 is 50-60%.
5. the processing method as described in any one of claim 1-4, it is characterised in that: in described step A4, described dilute alcohol is water-soluble
Alcohol in liquid is any one in methanol, ethanol, isopropanol, normal propyl alcohol, isobutanol, sec-butyl alcohol or n-butyl alcohol, most preferably
N-butyl alcohol.
6. the processing method as described in any one of claim 1-5, it is characterised in that: in described step A4, described percent mass
Water content is 65-80%, such as, can be 65%, 70%, 75% or 80%, most preferably 70%.
7. a breeding method for Rhizoma Et Radix Notopterygii seedling, described breeding method comprises the steps:
B1: according to the processing method of any one of claim 1-6, thus obtain the Rhizoma Et Radix Notopterygii seed for directly sowing；
B2: the plastics in seedling growth greenhouse are cultivated and are loaded compound material and mixed soil humous in bag, then by Rhizoma Et Radix Notopterygii seed points
It is sowed in described cultivation bag；
B3: keeping the percent mass water content cultivating the mixed soil in bag is 30-50%, and maintain the relative humidity of air to be
70-80%, shade rate are 70-85%；
B4: wait emerge neat after, just will cultivate bag integral transplanting to field, thus complete the cultivation of Rhizoma Et Radix Notopterygii seedling.
8. breeding method as claimed in claim 7, it is characterised in that: in described step B2, described compound material is with weight portion
Meter, the concrete component including following:
9. breeding method as claimed in claim 7, it is characterised in that: described Modification of kaolin fertilizer slow release granule is according to bag
Include what the preparation method of following steps obtained:
C1: Kaolin is calcined 50-70 minute at 500-600 DEG C, is crushed to 50-100 mesh after natural cooling, after being calcined
Powder；
C2: powder after described calcining is joined in the mixed aqueous solution of potassium nitrate, ammonium phosphate and ammonium chloride three, and at energy
Density be 2-3Kw/L ultrasound condition under dispersed with stirring 10-20 minute, obtain dispersion soln；
C3: by dispersion soln under agitation, is evaporated in 50-60 DEG C, then carries out pelletize, and obtaining granularity is 1-1.5mm's
Granule, dries completely, obtains described Modification of kaolin fertilizer slow release granule.
10. as claimed in claim 8 or 9 breeding method, it is characterised in that: described plant fermentation become thoroughly decomposed material be according to include as
The preparation method of lower step obtains:
D1: by 30 weight portion rice straw powder, 4 weight portion peanut hull meals, 6 weight portion Dry chick faeces, 2 weight portion peanut cakes, 2 weights
Amount part drying bagasse, 1.5 parts by weight Corn powder, 1 weight portion bean cake, 0.5 parts sulfuric acid potassium, 15 weight parts waters mix
Closing, after mix homogeneously, be stacked in long 5 meters, the fermentation tank of wide 1.5 meters, ulking thickness is 1 meter, obtains stockpile；
D2: 0.1 weight portion bacillus cereus and 0.05 weight portion yeast are put in 10 weight parts waters, after being uniformly dispersed, uniformly
Spray on stockpile；Then stockpile heats up naturally, control centre's temperature after 50-55 DEG C of bottom fermentation 2-3 days, abundant stirring,
To Preliminary fermentation material；
D3: join in 10 weight parts waters by 0.04 weight portion EM bacterium, after being uniformly dispersed, uniformly sprays on Preliminary fermentation material,
After central temperature reaches 75 DEG C, this temperature is maintained to fluctuate up and down less than 3 DEG C, and heap fermentation 30-35 hour, finally go out
Groove, dries, and obtains described plant fermentation and becomes thoroughly decomposed material.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980520A (en) * | 2017-12-07 | 2018-05-04 | 白兰香 | A kind of notopterygium root cultural method |
| CN108719072A (en) * | 2018-06-21 | 2018-11-02 | 四川千草生物技术股份有限公司 | Rhizoma Et Radix Notopterygii seed tissue culture fast breeding technology |
| CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049060A (en) * | 2007-04-30 | 2007-10-10 | 四川天农现代中药科技开发有限责任公司 | Breaking sleep and budding of notopterygium seeds |
| CN102657085A (en) * | 2012-05-10 | 2012-09-12 | 中国科学院西北高原生物研究所 | In-vitro rapid propagation method for tissue culture of broad-leafed notopterygium |
| CN102812839A (en) * | 2012-09-03 | 2012-12-12 | 四川新荷花中药饮片股份有限公司 | Cultivating method of notopterygium root |
| KR101432471B1 (en) * | 2014-03-27 | 2014-08-21 | 전라북도(농업기술원) | Bed soil for growing Gastrodia elata Blume and the method for preparing thereof and the method of growing Gastrodia elata Blume using the same |
| CN105684597A (en) * | 2016-01-28 | 2016-06-22 | 临洮农科教中药材开发有限公司 | Notopterygium root seed processing method and black mulching film land cultivating method of notopterygium roots |
-
2016
- 2016-06-25 CN CN201610474418.5A patent/CN106069333B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049060A (en) * | 2007-04-30 | 2007-10-10 | 四川天农现代中药科技开发有限责任公司 | Breaking sleep and budding of notopterygium seeds |
| CN102657085A (en) * | 2012-05-10 | 2012-09-12 | 中国科学院西北高原生物研究所 | In-vitro rapid propagation method for tissue culture of broad-leafed notopterygium |
| CN102812839A (en) * | 2012-09-03 | 2012-12-12 | 四川新荷花中药饮片股份有限公司 | Cultivating method of notopterygium root |
| KR101432471B1 (en) * | 2014-03-27 | 2014-08-21 | 전라북도(농업기술원) | Bed soil for growing Gastrodia elata Blume and the method for preparing thereof and the method of growing Gastrodia elata Blume using the same |
| CN105684597A (en) * | 2016-01-28 | 2016-06-22 | 临洮农科教中药材开发有限公司 | Notopterygium root seed processing method and black mulching film land cultivating method of notopterygium roots |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980520A (en) * | 2017-12-07 | 2018-05-04 | 白兰香 | A kind of notopterygium root cultural method |
| CN108719072A (en) * | 2018-06-21 | 2018-11-02 | 四川千草生物技术股份有限公司 | Rhizoma Et Radix Notopterygii seed tissue culture fast breeding technology |
| CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
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