CN1762205A - Generation method in the bottle of oriental hybrid lily detoxified small seed ball - Google Patents
Generation method in the bottle of oriental hybrid lily detoxified small seed ball Download PDFInfo
- Publication number
- CN1762205A CN1762205A CN 200410079513 CN200410079513A CN1762205A CN 1762205 A CN1762205 A CN 1762205A CN 200410079513 CN200410079513 CN 200410079513 CN 200410079513 A CN200410079513 A CN 200410079513A CN 1762205 A CN1762205 A CN 1762205A
- Authority
- CN
- China
- Prior art keywords
- ball
- bottle
- small seed
- lily
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a kind of interior generation method of bottle of oriental hybrid lily detoxified small seed ball, its is selected, sterilizes through outer tree planting body, and inducing culture obtains the detoxification stem apex, and again through enrichment culture, successive transfer culture directly generates detoxified small seed ball in bottle.It is long to the present invention is directed to the oriental hybrid lily balling-up cycle, and virus is difficult point such as accumulation easily, makes oriental hybrid lily direct generation detoxified small seed ball in bottle, can directly plant down behind the bottle outlet, and the transplanting survival rate height.By the production routine of whole lily ball being adjusted and being optimized; simplified the cultivation program of detoxification kind ball; shortened the cultivation time of oriental hybrid lily detoxification greatly; when having reduced production cost, improved kind ball quality; strengthened the homemade market competitiveness, be suitable for the large-scale production of oriental hybrid lily detoxification kind ball from the breeding ball.
Description
Technical field
The present invention relates to a kind of method of in blake bottle, producing the oriental hybrid lily detoxified small seed ball, belong to lily ball raising technology field.
Background technology
Lily (Lilium) is the perennial herb bulb bearing plant of Liliaceae lilium, and is elegant because it spends big look U.S., is world-renowned ornamental flower, and for many years, its cut-flower sales volume has been occupied preceding four of global fresh cut-flowers sales volume always.And oriental hybrid lily hybrid system (Oriental hybrids) is the maximum and the most beautiful big class of flower in the lily hybrid class, the flower type is bowl type or starlike bowl type, can give out pleasant fragrance, so be referred to as Fragrant Lily again, very popular, be that a big class lily hybrid of extensively cultivating in the world is.In the cultivating process of lily ball, the production cycle of lilium oriental is longer, needs 2.5～3 years by the scale cottage propagation to the cultivation of the commodity ball of blooming.Virus accumulation in the conventional vegetative propagation simultaneously is the big factor that restriction kind of ball breeding, cultivation and cut-flower are produced, so the production of virus-elimination seedlings (ball) is one of the most basic key measure of control lily disease viral disease with using.In the production process of virus-elimination seedlings (ball), existing report is all bred and is produced with detoxic seedling, does not see the report that directly generates detoxified small seed ball in bottle.
Summary of the invention
The object of the present invention is to provide and a kind ofly can effectively shorten cultivation period, reduce the chance of viral cross-infection, improve generation method in the bottle of oriental hybrid lily detoxified small seed ball of lily ball quality.
The present invention finishes by following technical proposal: generation method in a kind of bottle of oriental hybrid lily detoxified small seed ball is characterized in that through the following step:
1), the selection of explant and sterilization: select no damage by disease and insect, robust growth, do not detect the lily ball of CMV (cucumber mosaic virus) and LSV (lily conceals syndrome virus), under 3～6 ℃ low temperature, dark condition, preserve the back taking-up of 6～8 weeks, stripping scale limit, limit is washed with running water, after removing outside 2～3 layers of scale that wraps up consolidation not, with concentration is that 0.1% washing powder water logging bubble is after 2～5 minutes, clean up mercuric chloride (Hgcl 0.1～0.2% with running water
2) sterilized 15～25 minutes in the solution, in 1～3% liquor natrii hypochloritis, sterilized 10～15 minutes then, with aseptic washing 3～5 times;
2), inducing culture: under aseptic condition, the scale stripping and slicing after the sterilization being inoculated in the following inducing culture and cultivating, is 25 ± 2 ℃ in temperature, light application time 10～12h/d, under the environmental condition of intensity of illumination 2000～3000Lx, behind cultivation 20～25d, generate indefinite bud in incision:
The MS basic culture solution
6-benzylaminopurine (6-BA) 1.0～2.0mg/L
Methyl (NAA) 0.1～0.5mg/L
Sucrose 30000～40000mg/L
Agar 5000～5500mg/L
pH 5.5～6.0
3), obtain the detoxification stem apex: above-mentioned indefinite bud of inducing generation after 36～40 ℃ of condition therapeutic method to keep the adverse qi flowing downward heat are cultivated 10～30 days, is stripped the stem apex that obtains 0.2～0.5mm under anatomical lens;
4), enrichment culture: under aseptic condition, the detoxification stem apex that step 3) is obtained changes in the following proliferated culture medium:
The MS basic culture solution
6-benzylaminopurine (6-BA) 1.0～2.0mg/L
Methyl (NAA) 0.2～0.5mg/L
Sucrose 30000～40000mg/L
Agar 5000～6000mg/L
pH 5.5～6.0
In temperature is 26 ± 2 ℃, and light application time 10～12 hours/day under the condition of intensity of illumination 1500～2000Lx, is cultivated 20～25d, makes 1 stem apex grow into a plurality of indefinite buds;
5), successive transfer culture: the indefinite bud of above-mentioned propagation divided be cut to simple bud or 2～3 clump under aseptic condition, insert in the same proliferated culture medium, under identical culture environment, cultivate, can obtain a large amount of unrooted indefinite buds, the leaf look of propagation seedling is dark green, robust growth, base portion are expanded into bulb shape;
6), directly generate detoxified small seed ball in the bottle: the indefinite bud that subculture is formed cuts blade, and base portion and scale stripping and slicing insert in the following green-ball medium:
The MS basic culture solution
Methyl (NAA) 0.05～0.1mg/L
Sucrose 80000～120000mg/L
Agar 4500～6000mg/L
Active carbon 500～600mg/L
pH 5.5～6.0
In temperature is 20 ± 2 ℃, under the dark condition, cultivates 60～70d, directly generates diameter 0.8～1.2cm around the indefinite bud stripping and slicing reaches, the small seed ball of girth diameter 3.0～3.5cm, and each stripping and slicing can generate 2～4 of small seed balls.
The invention solves following technological difficulties:
1, long at the oriental hybrid lily balling-up cycle, virus is the difficult point of accumulation easily, the present invention directly generates small seed ball by said method in bottle, its look white, the scale consolidation, on-bladed can directly be planted down behind the bottle outlet, and diameter, girth diameter have reached conventional tissue cultivating seedling at the small seed ball of field growing more than 3 months, shorten the cultivation period of detoxification kind ball greatly, reduced production cost.Small seed ball on-bladed behind the bottle outlet is easy to plant preceding refrigeration and handles simultaneously, and survival rate is higher after the field planting.
2, in Plant Tissue Breeding, the plant in the blake bottle is all based on heterotrophic growth, and sugar is the main source that plant carries out the carbonizable substance accumulation.Second difficult point that the present invention solves is: by add the sucrose (8～12g/L) of high concentration in solid or liquid nutrient medium, the soluble sugar that indefinite bud is absorbed is accumulate in the small seed ball rapidly, detoxified small seed ball in the bottle has obtained high-speed rapid growth, has improved the quality of small seed ball simultaneously.
3, in the group of routine training detoxic seedling is cultivated, indefinite bud need induce the cultivation of the plant that takes root again behind the blade.Yet usually because the deficiency of the interior CO2 gas of blake bottle, make the tissue cultivating seedling blade in the blake bottle not only can not normally carry out photosynthesis accumulation photosynthetic product, also opposite because the existence of blade has consumed the nutrient that base portion seed ball is accumulated, the growth of group training small seed ball is suppressed.In addition, the illumination that provides in the conventional condition of tissue culture only is that the chlorophyll in the maintenance group training blade generates, and does not have value in the small seed ball of lily generates.Therefore in the present invention, the 3rd difficult point that solves is: take to carry out the cultivation of detoxified small seed ball under complete dark condition, suppress leaf growth, avoid nutrient consumption, thereby the nutrient in the medium all is accumulate in the small seed ball, has improved the growth rate of small seed ball.
4, the present invention is at different cultivation stages, according to different culturing purposes, key factors such as medium, sucrose concentration, interpolation active carbon and condition of culture are regulated and control, both reached high efficiently multiplying, guaranteed the quality of small seed ball again, especially in the direct green-ball stage, adopt complete dark condition to cultivate 60～70d, not only saved the consumption of the energy, and culture environment approaches the environment that the scale ball " is buried sheet " and generates in the lily cuttage, handle easily behind the seed ball bottle outlet, transplanting survival rate is high and be beneficial to late growing stage.
5, the present invention utilizes in the tissue culture clean environment, makes a bottle interior detoxified small seed ball not have damage by disease and insect, and transplant survival is easy, is convenient to the international even at home interchange of lily germ plasm resource, has avoided the exchange of sick worm by germplasm that great outburst takes place.
The present invention has following advantage and effect: by directly generating the detoxified small seed ball technology in the bottle of oriental hybrid lily; the production routine of whole lily ball is adjusted and optimize; simplified the cultivation program of detoxification kind ball; shortened the cultivation time of oriental hybrid lily detoxification greatly; when having reduced production cost, improved kind ball quality; strengthened the homemade market competitiveness, be suitable for the large-scale production of oriental hybrid lily detoxification kind ball from the breeding ball.
The present invention studies the key factor that directly generates in this step of detoxified small seed ball in the oriental hybrid lily bottle emphatically, and specifically, just from the regulation and control two broad aspect research experiment of concentration of sucrose and culture environment, its result is reported as follows:
1, test material: the kind ball of getting oriental hybrid lily kind " Tiber " is as supplying the examination material.
2, method and result
The same embodiment of whole link of the acquisition of the selection of explant, sterilization, detoxification stem apex and propagation, subculture, primary study the direct generation of detoxified small seed ball in bottle.
(1) sucrose concentration is to the influence of small seed ball generation in the bottle
Test different sucrose concentrations to directly generating the influence of small seed ball in the bottle, designed 2%～14% a series of gradient concentration, under dark fully condition of culture, cultivate 60d, observe the growing state and the seed ball size of seed ball, the measurement of average diameter is after measuring the diameter of 20 small seed balls with ruler, average, the measurement of average girth diameter measures numerical value with ruler more earlier with the maximum of thin cotton thread around the seed ball, get the mean value of 20 small seed balls equally, result of the test sees Table 1.
By above comparative test, draw sucrose concentration 10% when following, the increase and the sucrose concentration of seed ball size are proportionate, all reached optimum to 10% o'clock every index, the seed nodule number amount of production is moderate, and diameter and girth diameter have reached maximum, after surpassing 10%, though the every formed seed nodule number of explant amount increases to some extent, the index of single seed ball descends on the contrary to some extent, and the shape of growing thickly, be unfavorable for the separation and the transplanting of seed ball, to 14% o'clock, existing part seed ball distorted flat.Therefore, in the Orient in the lily bottle directly in the method for generation detoxified small seed ball, more suitable to add 8%～12% sucrose in water ratio.
(2) regulation and control of culture environment
Adopt the above-mentioned the suitableeest sucrose concentration that mixes, stripping and slicing is inserted in the green-ball medium of MS+NAA0.1+ sucrose 10%+ active carbon 0.6%, different below condition of culture (mainly being the adjustment of illumination), temperature is to cultivate in 20 ± 2 ℃ the environment, measure every index of seed ball behind the 60d, concrete outcome sees Table 2.
Table 1: the upgrowth situation of different sucrose seeding down ball
| Sucrose concentration % | The every average small seed ball quantity that explant generates | Average diameter cm | Average girth diameter cm | Upgrowth situation |
| 2 | 1.1 | 0.68 | 0.78 | Radical is few and thick long, 2～3 on leaf is arranged on every young plant, the scale parcel of seed ball is a solid, not like normal lily bulb |
| 4 | 1.6 | 0.80 | 1.12 | Radical is few and thick long, 2～3 on leaf is arranged, the visible squamose differentiation of seed ball on every young plant |
| 6 | 2.3 | 0.92 | 2.02 | Root is few and elongated, and 1～2 on leaf is arranged on every young plant, and the scale of seed ball differentiation is more, approaches normal bulb |
| 8 | 2.8 | 1.01 | 3.17 | Root is many and elongated, and 1～2 on leaf is arranged on every young plant, and each seed ball differentiates 10～15 scales |
| 10 | 3.7 | 1.12 | 3.45 | Root is many and elongated, does not form blade, and each seed ball differentiates 15～20 scales, scale carnification, white |
| 12 | 4.1 | 0.96 | 2.58 | Root is slightly lacked, and does not form blade, and each seed ball differentiates 10～12 scales, and quantity is more, but smaller |
| 14 | 5.8 | 0.78 | 1.07 | Root is slightly lacked, do not formed blade, each seed ball only differentiates 6～7 scales, the seed ball is from giving birth to shape, shaped flat and fractional distortion, quantity is a lot, but smaller |
The condition of culture that finally draws the generation of oriental hybrid lily detoxified small seed ball by above test is better to carry out culture effect under the condition of complete dark, and the seed nodule number amount of generation is moderate, and girth diameter is the highest in test combinations greater than the ratio of 3cm simultaneously, seed ball Functionality, quality and appealing design.
Table 2: the upgrowth situation of detoxified small seed ball under the different condition of culture
| Light intensity lx | Light application time h/d | The every average small seed ball quantity that explant generates | Girth diameter is greater than the shared ratio % of 3 cm seed balls | Upgrowth situation |
| 2000 | 24 | 4.02 | 34 | The seed ball is dark green and some is rubescent, the small seed ball quantity that forms is many, but girth diameter is in the majority less than the quantity of 1 cm, some greening of root, 2～3 on leaf is arranged, dark green leaf color on every young plant |
| 2000 | 12 | 4.56 | 61 | The seed ball is dark green, and the small seed ball quantity of formation is many, and 1～2 on leaf is arranged on every young plant, and the leaf look light is slightly |
| 0 | 0 | 3.42 | 87 | Seed ball white, more sturdy, the scale consolidation, the small seed ball quantity of formation is moderate, on-bladed |
Embodiment
For flesh and blood of the present invention is described better, provide embodiments of the invention below, but content of the present invention is not limited in these.
Embodiment 1
1), the selection of explant and sterilization: preferred growth stalwartness, blooming property Like is good, no damage by disease and insect, the kind ball of the oriental hybrid lily kind " Tiber " of virus-free symptom performance is as explant, before drawing materials, with the bulb of preparing inoculation low temperature at 4 ℃, dark condition is the back taking-up of 8 week of storage down, stripping scale limit, limit is washed with running water, after removing bulb crust and outside 2～3 layers of scale that wraps up consolidation not, with concentration is 0.1% washing powder water logging bubble 2 minutes, running water cleans up, cut a little top and base portion, shell into the single scale of band portion basal disc, mercuric chloride (Hgcl 0.1%
2) sterilization 25 minutes in the solution, sterilization is 15 minutes among 1% the liquor natrii hypochloritis, behind the aseptic water washing 3 times, and scale stripping and slicing inoculation;
2), inducing culture: under aseptic condition, the scale stripping and slicing after the sterilization is inoculated in the following inducing culture:
The MS basic culture solution
6-benzylaminopurine (6-BA) 1.0mg/L
Methyl (NAA) 0.1mg/L
Sucrose 30000mg/L
Agar 5000mg/L
pH 5.5
In temperature is 25 ± 2 ℃, and light application time 12h/d under the environmental condition of intensity of illumination 2000Lx, cultivates 25d, directly generates indefinite bud in incision, or forms indefinite bud by the callus dedifferentiation;
3), the acquisition of detoxification stem apex: the above-mentioned indefinite bud of generation of inducing was cultivated 30 days 36 ℃ of condition therapeutic method to keep the adverse qi flowing downward heat, under anatomical lens, stripped the detoxification stem apex that obtains 0.2～0.5mm;
4), enrichment culture: under aseptic condition, with the 3rd) the detoxification stem apex of step acquisition changes in the following proliferated culture medium:
The MS basic culture solution
6-benzylaminopurine (6-BA) 1.0mg/L
Methyl (NAA) 0.2mg/L
Sucrose 30000mg/L
Agar 5000mg/L
pH 5.5
In temperature is 26 ± 2 ℃, and light application time 10 hours/day under the condition of intensity of illumination 2000Lx, is cultivated 20d, can make stem apex be grown to indefinite bud, and can on average breed by 1 is 4;
5), successive transfer culture: with the 4th) the resulting indefinite bud of step propagation divides under aseptic condition and is cut to one clump of simple bud or 2～3 bud, insert in the same proliferated culture medium, under identical culture environment, cultivate, can obtain a large amount of unrooted indefinite buds, the leaf look of propagation seedling is dark green, robust growth, base portion are expanded into bulb shape;
6), directly generate detoxified small seed ball in the bottle: the indefinite bud that subculture is formed cuts blade, and base portion and scale stripping and slicing insert following green-ball medium:
The MS basic culture solution
Methyl (NAA) 0.05mg/L
Sucrose 80000mg/L
Agar 4500mg/L
Active carbon 500mg/L
pH 5.5
In temperature is 20 ± 2 ℃, under the dark condition, cultivates 70d, and the indefinite bud stripping and slicing can directly generate small seed ball around reaching, and average diameter reaches 1cm, and average girth diameter reaches more than the 3.0cm, and each stripping and slicing on average can generate 2.8 of small seed balls.
Embodiment 2
1), preferred growth stalwartness, good, the no damage by disease and insect of the proterties of blooming, the kind ball of the oriental hybrid lily kind " Acapulco " of virus-free symptom performance is as explant, before drawing materials, with the bulb of preparing inoculation low temperature at 6 ℃, dark condition is the back taking-up of 8 week of storage down, stripping scale limit, limit is washed with running water, after removing bulb crust and outside 2～3 layers of scale that wraps up consolidation not, steeped 5 minutes with 0.1% washing powder water logging, running water cleans up, cut a little top and base portion, shell into the single scale of band portion basal disc, mercuric chloride (Hgcl 0.2%
2) handled 15 minutes in the solution, handled 10 minutes among 3% the liquor natrii hypochloritis, behind the aseptic water washing 5 times, scale stripping and slicing inoculation;
2), inducing culture: under aseptic condition, the scale stripping and slicing after the sterilization is inoculated in the following inducing culture:
The MS basic culture solution
6-benzylaminopurine (6-BA) 2.0mg/L
Methyl (NAA) 0.5mg/L
Sucrose 40000mg/L
Agar 5500mg/L
pH 6.0
In temperature is 25 ± 2 ℃, and light application time 10h/d under the environmental condition of intensity of illumination 3000Lx, cultivates 20d, directly generates indefinite bud in incision, or forms indefinite bud by the callus dedifferentiation;
3), the acquisition of detoxification stem apex: with the 2nd) indefinite bud that produces of one-step inducing makes material, in the lily plantation, produce the bigger virus disease of harm, cultivate 10 days by 40 ℃ of gas heat after, under anatomical lens, strip the detoxification stem apex that obtains 0.2～0.5mm;
4), enrichment culture: under aseptic condition, with the 3rd) the detoxification stem apex of step acquisition changes in the following proliferated culture medium:
The MS basic culture solution
6-benzylaminopurine (6-BA) 2.0mg/L
Methyl (NAA) 0.5mg/L
Sucrose 40000mg/L
Agar 6000mg/L
pH 6.0
In temperature is 26 ± 2 ℃, and light application time 12 hours/day under the condition of intensity of illumination 1500Lx, is cultivated 25d, makes stem apex be grown to indefinite bud, and can on average breed by 1 is 5;
5), successive transfer culture: with the 4th) the resulting indefinite bud of step propagation divides under aseptic condition and is cut to one clump of simple bud or 2～3 bud, insert in the same proliferated culture medium, under identical culture environment, cultivate, can obtain a large amount of unrooted indefinite buds, the leaf look of propagation seedling is dark green, robust growth, base portion are expanded into bulb shape;
6), directly generate detoxified small seed ball in the bottle: the indefinite bud that subculture is formed cuts blade, and base portion and scale stripping and slicing insert following green-ball medium:
The MS basic culture solution
Methyl (NAA) 0.1mg/L
Sucrose 120000mg/L
Agar 6000mg/L
Active carbon 600mg/L
pH 6.0
In temperature is 20 ± 2 ℃, under the dark condition, cultivates 70d, directly generates little young ball around the indefinite bud stripping and slicing reaches, and average diameter is 0.96cm, and average girth diameter 2.58cm on average can generate 4 of small seed balls.
Claims (1)
1, generation method in a kind of bottle of oriental hybrid lily detoxified small seed ball is characterized in that through the following step:
1), the selection of explant and sterilization: select no damage by disease and insect, robust growth, do not detect the lily ball of CMV (cucumber mosaic virus) and LSV (lily conceals syndrome virus), under 3～6 ℃ low temperature, dark condition, preserve the back taking-up of 6～8 weeks, stripping scale limit, limit is washed with running water, after removing outside 2～3 layers of scale that wraps up consolidation not, with concentration is that 0.1% washing powder water logging bubble is after 2～5 minutes, clean up mercuric chloride (Hgcl 0.1～0.2% with running water
2) sterilized 15～25 minutes in the solution, in 1～3% liquor natrii hypochloritis, sterilized 10～15 minutes then, with aseptic washing 3～5 times;
2), inducing culture: under aseptic condition, the scale stripping and slicing after the sterilization being inoculated in the following inducing culture and cultivating, is 25 ± 2 ℃ in temperature, light application time 10～12h/d, under the environmental condition of intensity of illumination 2000～3000Lx, behind cultivation 20～25d, generate indefinite bud in incision:
The MS basic culture solution
6-benzylaminopurine (6-BA) 1.0～2.0mg/L
Methyl (NAA) 0.1～0.5mg/L
Sucrose 30000～40000mg/L
Agar 5000～5500mg/L
pH 5.5～6.0
3), obtain the detoxification stem apex: above-mentioned indefinite bud of inducing generation after 36～40 ℃ of condition therapeutic method to keep the adverse qi flowing downward heat are cultivated 10～30 days, is stripped the stem apex that obtains 0.2～0.5mm under anatomical lens;
4), enrichment culture: under aseptic condition, the detoxification stem apex that step 3) is obtained changes in the following proliferated culture medium:
The MS basic culture solution
6-benzylaminopurine (6-BA) 1.0～2.0mg/L
Methyl (NAA) 0.2～0.5mg/L
Sucrose 30000～40000mg/L
Agar 5000～6000mg/L
pH 5.5～6.0
In temperature is 26 ± 2 ℃, and light application time 10～12 hours/day under the condition of intensity of illumination 1500～2000Lx, is cultivated 20～25d, makes 1 stem apex grow into a plurality of indefinite buds;
5), successive transfer culture: the indefinite bud of above-mentioned propagation divided be cut to simple bud or 2～3 clump under aseptic condition, insert in the same proliferated culture medium, under identical culture environment, cultivate, can obtain a large amount of unrooted indefinite buds, the leaf look of propagation seedling is dark green, robust growth, base portion are expanded into bulb shape;
6), directly generate detoxified small seed ball in the bottle: the indefinite bud that subculture is formed cuts blade, and base portion and scale stripping and slicing insert in the following green-ball medium:
The MS basic culture solution
Methyl (NAA) 0.05～0.1mg/L
Sucrose 80000～120000mg/L
Agar 4500～6000mg/L
Active carbon 500～600mg/L
pH 5.5～6.0
In temperature is 20 ± 2 ℃, under the dark condition, cultivates 60～70d, directly generates diameter 0.8～1.2cm around the indefinite bud stripping and slicing reaches, the small seed ball of girth diameter 3.0～3.5cm, and each stripping and slicing can generate 2～4 of small seed balls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100795132A CN100394845C (en) | 2004-10-21 | 2004-10-21 | In-bottle production method of detoxified small seed ball of east lily |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100795132A CN100394845C (en) | 2004-10-21 | 2004-10-21 | In-bottle production method of detoxified small seed ball of east lily |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1762205A true CN1762205A (en) | 2006-04-26 |
| CN100394845C CN100394845C (en) | 2008-06-18 |
Family
ID=36746685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100795132A Expired - Fee Related CN100394845C (en) | 2004-10-21 | 2004-10-21 | In-bottle production method of detoxified small seed ball of east lily |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100394845C (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116424B (en) * | 2007-09-04 | 2010-05-19 | 云南省农业科学院花卉研究所 | Highly effective lily bulblet inducement culture method |
| CN101156552B (en) * | 2007-11-02 | 2010-06-02 | 云南格桑花卉有限责任公司 | Oriental lily tissue culture detoxicating method |
| CN101133717B (en) * | 2007-08-22 | 2011-05-04 | 南京林业大学 | Highly-efficient regeneration method for Siberia lily detoxification test tube plantlet squama bulb from the bulb flake |
| CN102405845A (en) * | 2011-12-12 | 2012-04-11 | 中山火炬职业技术学院 | Tissue culture rapid propagation method of ornamental lily |
| CN102440184A (en) * | 2010-10-14 | 2012-05-09 | 王文和 | Culturing method for utilizing bioreactor to quickly expand lily detoxified cormels |
| CN102440185A (en) * | 2010-10-14 | 2012-05-09 | 赵祥云 | Fast culturing method for lily virus-free seed ball |
| CN102754598A (en) * | 2012-07-10 | 2012-10-31 | 中国长江三峡集团公司 | Method for inducing test-tube bulblet of lily |
| CN104304030A (en) * | 2014-10-31 | 2015-01-28 | 浙江大学宁波理工学院 | Propagation method of oriental lily |
| CN105613283A (en) * | 2014-10-27 | 2016-06-01 | 松桃宏发肉食品有限责任公司 | Fast culturing method for lily virus-free bulblets |
| CN107258540A (en) * | 2017-07-26 | 2017-10-20 | 株洲市农业科学研究所 | The tissue culture method of fast breeding oriental hybrid lily hybrid new breed |
| CN109463277A (en) * | 2018-11-23 | 2019-03-15 | 北京农学院 | Hybrid lily offspring's high-efficiency in-vitro balling-up and the method for shortening juvenile phase |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193656C (en) * | 2003-06-04 | 2005-03-23 | 中国科学院昆明植物研究所 | Quick breeding method of lanzhou lily |
-
2004
- 2004-10-21 CN CNB2004100795132A patent/CN100394845C/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133717B (en) * | 2007-08-22 | 2011-05-04 | 南京林业大学 | Highly-efficient regeneration method for Siberia lily detoxification test tube plantlet squama bulb from the bulb flake |
| CN101116424B (en) * | 2007-09-04 | 2010-05-19 | 云南省农业科学院花卉研究所 | Highly effective lily bulblet inducement culture method |
| CN101156552B (en) * | 2007-11-02 | 2010-06-02 | 云南格桑花卉有限责任公司 | Oriental lily tissue culture detoxicating method |
| CN102440185B (en) * | 2010-10-14 | 2015-05-27 | 赵祥云 | Fast culturing method for lily virus-free seed ball |
| CN102440184A (en) * | 2010-10-14 | 2012-05-09 | 王文和 | Culturing method for utilizing bioreactor to quickly expand lily detoxified cormels |
| CN102440185A (en) * | 2010-10-14 | 2012-05-09 | 赵祥云 | Fast culturing method for lily virus-free seed ball |
| CN102405845A (en) * | 2011-12-12 | 2012-04-11 | 中山火炬职业技术学院 | Tissue culture rapid propagation method of ornamental lily |
| CN102754598A (en) * | 2012-07-10 | 2012-10-31 | 中国长江三峡集团公司 | Method for inducing test-tube bulblet of lily |
| CN105613283A (en) * | 2014-10-27 | 2016-06-01 | 松桃宏发肉食品有限责任公司 | Fast culturing method for lily virus-free bulblets |
| CN104304030A (en) * | 2014-10-31 | 2015-01-28 | 浙江大学宁波理工学院 | Propagation method of oriental lily |
| CN104304030B (en) * | 2014-10-31 | 2016-06-29 | 浙江大学宁波理工学院 | A kind of propagation method of oriental hybrid lily |
| CN107258540A (en) * | 2017-07-26 | 2017-10-20 | 株洲市农业科学研究所 | The tissue culture method of fast breeding oriental hybrid lily hybrid new breed |
| CN109463277A (en) * | 2018-11-23 | 2019-03-15 | 北京农学院 | Hybrid lily offspring's high-efficiency in-vitro balling-up and the method for shortening juvenile phase |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100394845C (en) | 2008-06-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101213942B (en) | Medicinal anoectochilus Formosan tissue culture one-step seedling establishment fast propogation method | |
| CN103858762A (en) | Rapid proliferation method for anoectochilus formosanus tissue culture | |
| CN106358825A (en) | Recyclable cutting substance for growing lithocarpus polystachyus seedlings and application of recyclable cutting substance | |
| CN101049090A (en) | Method for carrying out taking off poison and quick breeding by using strawberry anther | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN100581353C (en) | Artificial rapid reproduction method for rutaceae zanthoxylum plant zanthoxylum dissitum Hemsl | |
| CN1799344A (en) | Artificial quick propagation method for hemsley rockvine root | |
| CN101044840A (en) | Method for fast breeding western azalea and culture medium therefor | |
| CN102047842A (en) | Method for directly regenerating plants by adopting citrullus lanatus cotyledon nodes | |
| CN1762205A (en) | Generation method in the bottle of oriental hybrid lily detoxified small seed ball | |
| CN103651136A (en) | Rhynchostylis tissue culture method | |
| CN101548646B (en) | Method for rapidly propagating aralia elata through somatic embryo and secondary somatic embryogenesis | |
| CN1613297A (en) | Method for tissue culture of lily flowers | |
| CN103070078A (en) | Rapid propagation method for performing tissue culture by using taro stem tip | |
| CN105409748B (en) | A kind of fast breeding method of extra large scirpus scirpus | |
| CN104686344A (en) | Tissue culture method of liriope muscari | |
| CN1695429A (en) | Quick method for breeding rosewood | |
| CN104429958B (en) | The method of rejuvenation in American elm tissue cultured seedling bottle | |
| CN104396746B (en) | A kind of chrysanthemum bulb of fritillary adventitious bud inducing enrichment procedure | |
| CN108142281A (en) | A kind of Cortex Eucommiae method for tissue culture | |
| CN106386504A (en) | Tissue culture method of Aralia Cordata Thunb seedlings | |
| CN109588312A (en) | A kind of method that calanthe plant regeneration system is established | |
| CN109392717A (en) | A kind of cortex cinnamomi tissue culture and rapid propagation method | |
| CN1739341A (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN103548653A (en) | Honeysuckle tissue culture seedling non-tube rootage method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Yuxi Ming Zhu Flower Co., Ltd. Assignor: Flower Research Institute(FRI) of Yunnan Contract record no.: 2011530000024 Denomination of invention: In-bottle production method of detoxified small seed ball of east lily Granted publication date: 20080618 License type: Exclusive License Open date: 20060426 Record date: 20110601 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080618 Termination date: 20141021 |
|
| EXPY | Termination of patent right or utility model |