CN105010109B - The ciltivating process of Momordica grosvenori - Google Patents

The ciltivating process of Momordica grosvenori Download PDF

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Publication number
CN105010109B
CN105010109B CN201510385367.4A CN201510385367A CN105010109B CN 105010109 B CN105010109 B CN 105010109B CN 201510385367 A CN201510385367 A CN 201510385367A CN 105010109 B CN105010109 B CN 105010109B
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momordica grosvenori
nutrient solution
culture nutrient
rooted seedling
water culture
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CN105010109A (en
Inventor
潘丽梅
韦荣昌
马小军
白隆华
莫长明
冯世鑫
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Guangxi Botanical Garden of Medicinal Plants
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Guangxi Botanical Garden of Medicinal Plants
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

Abstract

The invention discloses a kind of ciltivating process of Momordica grosvenori, comprise the following steps, transplant:The base portion of Momordica grosvenori rooted seedling after sterilization is coated with sponge, is transplanted into afterwards in the cultivation bed for containing mill water culture nutrient solution, makes the 1/2~5/6 of the root system of Momordica grosvenori rooted seedling to immerse in mill water culture nutrient solution;Water planting management:Momordica grosvenori is taken root after transplantation of seedlings, between the pH value for keeping mill water culture nutrient solution is 5.5~6.5, is passed through air 30min into body daily, when the root system of mill water culture nutrient solution submergence Momordica grosvenori is no more than 1/3, then changes mill water culture nutrient solution.Using the Momordica grosvenori of water planting of the present invention, its mill water culture nutrient solution can meet plant strain growth demand, and the Momordica grosvenori through water planting culture, well developed root system, cane is sturdy, and leaf color is dark green, restoration ecosystem gesture that can be quickly when being transferred to field planting, meet production requirement.

Description

The ciltivating process of Momordica grosvenori
Technical field
The present invention relates to a kind of cultural method of Momordica grosvenori, it is more particularly related to a kind of water planting of Momordica grosvenori Method.
Background technology
Momordica grosvenori is the perennial liana of Curcurbitaceae Momordica grosvenori category, is Chinese distinctive medicinal and sweetener plant, no Only be the herbal species that pharmacopeia is recorded, and the Ministry of Public Health, State Administration of Traditional Chinese Medicine first to be included in be both food and medicine The Chinese medicine of register, it is the famous genunie medicinal materials in Guangxi.The sapling multiplication of Momordica grosvenori is mainly based on tissue cultures, therefore group at present It is most important to train the transplant survival of seedling, conventional tissue culture transplantation of seedlings is mainly to be transplanted to based on greenhouse nutrition cup, but liquid manure is applied With and bad control, the easy burn seedlings of premature application, excessively late apply then be difficult to meet growth require, growth retardation.The invention reside in A kind of Luohanguo With Plantlets of Tissue Culture water culture technique is provided, supplies water, fertilizer, gas, temperature, light by the physiological period of plant completely so that arhat Fruit tissue-cultured seedling well-grown, to improve the tissue-cultured seedling transplanting survival rate of Momordica grosvenori and the speed of growth.Simultaneously because water planting lean on be Artificial climate, do not influenceed, can be produced with the anniversary by Natural Water, temperature, light, broken the limitation in season, greatly improved The production efficiency of Momordica grosvenori seedling.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least will be described later it is excellent Point.
It is a still further object of the present invention to provide a kind of ciltivating process of Momordica grosvenori, pass through the base portion in Momordica grosvenori rooted seedling Sponge is coated, article scratch of the base portion by outside can be protected, Momordica grosvenori rooted seedling is suitable for growing environment.
A further object of the invention is to immerse the mill water culture nutrient solution by the 1/2~5/6 of the root system of Momordica grosvenori rooted seedling In, mill water culture nutrient solution is reserved a part of root not over the pseudobulb of Momordica grosvenori root and is breathed with air contact.
In order to realize according to object of the present invention and further advantage, there is provided a kind of ciltivating process of Momordica grosvenori, bag Include following steps:
Transplanting:The base portion of Momordica grosvenori rooted seedling after sterilization is coated with sponge, is transplanted into contains water planting battalion afterwards In the cultivation bed of nutrient solution, the 1/2~5/6 of the root system of Momordica grosvenori rooted seedling is set to immerse in the mill water culture nutrient solution;
Wherein, matched lid is provided with the cultivation bed, the lid is provided with a first through hole sum Individual aperture is 2~4cm the second through hole with the fixation Momordica grosvenori rooted seedling, and body is inserted with the first through hole, described The outlet side of body and the root of the Momordica grosvenori rooted seedling are in same level;
Water planting management:The Momordica grosvenori is taken root after transplantation of seedlings, keep the pH value of the mill water culture nutrient solution for 5.5~6.5 it Between, air 30min is passed through into the body daily, when the root system that mill water culture nutrient solution submerges the Momordica grosvenori is no more than 1/3, Then change the mill water culture nutrient solution.
Preferably, the breeding method of the Momordica grosvenori rooted seedling is the Momordica grosvenori that will be cultivated by subculture and leaf color is dark green Sterile tissue culture plant inoculation is cultivated to taking root, the naphthalene second containing MS, 0.2mg/L in the root media into root media The sucrose of acid, 0.1mg/L indolebutyric acid, 4.5g/L agar and 30g/L.
Preferably, the mill water culture nutrient solution per 10L contains following component:810~830mg of calcium nitrate tetrahydrate, nitre Sour 580~620mg of potassium, 140~160mg of ammonium dihydrogen phosphate, 365~375mg of magnesium sulfate, disodium ethylene diamine tetraacetate iron 13~ 19mg, four 0.6~0.8mg of hydrated manganese sulfate, 1.0~1.4mg of boric acid, 0.02~0.06mg of Salzburg vitriol, zinc sulfate 0.06~0.1mg and molybdic acid 0.01mg.
Preferably, the 10th~15 day after transplanting, beer waste water nutrient solution is substituted for by the mill water culture nutrient solution, its In, the beer waste water nutrient solution is pressed by beer waste water of the COD value less than or equal to 120mg/L, acidifying bacterium and trace element Mass ratio is 1000: 1~5: 10~15 compositions.
Preferably, the cultivation bed is glass material, and the cultivation bed that transplanting has Momordica grosvenori rooted seedling is placed in into Momordica grosvenori In culture apparatus, and magnetic stir bar is added in cultivation bed, wherein, the Momordica grosvenori culture apparatus includes light-passing board and carrying The magnetic stirring apparatus of the cultivation bed, the light-passing board and magnetic stirring apparatus are fixed by several support columns, the printing opacity LED light source is provided with plate, after Momordica grosvenori takes root transplantation of seedlings, daily with magnetic stir bar and magnetic stirring apparatus to the cultivation Mill water culture nutrient solution in training groove replaces or beer waste water nutrient solution stirring 2-3 hours.
Preferably, the Momordica grosvenori rooted seedling after transplanting maintain the temperature at 22~25 DEG C, intensity of illumination be 8200~ 8500Lx。
Preferably, the hydrogen-oxygen that the reagent for adjusting pH is the salpeter solution that mass fraction is 5% or mass fraction is 5% Change sodium solution.
Preferably, the method for sterilizing Momordica grosvenori rooted seedling is that Momordica grosvenori rooted seedling is put into mass fraction is 0.1% Soaked 10~20 minutes in carbendazim solution.
The present invention comprises at least following beneficial effect:
1st, plant strain growth demand can be met using the Momordica grosvenori of water planting of the present invention, its mill water culture nutrient solution, through water planting culture Momordica grosvenori, well developed root system, cane is sturdy, and leaf color is dark green, restoration ecosystem gesture that can be quickly when being transferred to field planting, meets production Demand.
2nd, sponge is coated by the base portion in Momordica grosvenori rooted seedling, article scratch of the base portion by outside can be protected, make arhat Fruit rooted seedling is suitable for growing environment.
The 3rd, a kind of special cultivation bed is set, by setting first through hole, eliminate the fixation to Momordica grosvenori rooted seedling, can The water planting of large area is carried out to Momordica grosvenori using this device, management is convenient.
4th, air is passed through to the root of the close Momordica grosvenori rooted seedling of cultivation bed, prevents the root anaerobic respiration mistake of Momordica grosvenori It is more, cause rotten so as to generate alcohol.
5th, the 1/2~5/6 of the root system of Momordica grosvenori rooted seedling is immersed in the mill water culture nutrient solution, makes mill water culture nutrient solution will not More than the pseudobulb of Momordica grosvenori root, reserve a part of root and breathed with air contact.
6th, at 10-15 days of Momordica grosvenori rooted seedling water planting, using beer waste water Solution culture method Momordica grosvenori rooted seedling, i.e., Discarded beer waste water is make use of, while the material in beer waste water can promote the speed of growth of Momordica grosvenori, shorten Momordica grosvenori The cultivation time.
7th, by Momordica grosvenori culture apparatus, the liquid in cultivation bed can be stirred, the root of Momordica grosvenori will not be injured, Also the liquid of inside is made to mix evenly, root absorption is more abundant, uses LED light source to set intensity of illumination as 8200-8500Lx It can meet needed for the growth of Momordica grosvenori.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Brief description of the drawings
Fig. 1 present invention is provided with the structure chart of the cultivation bed of lid;
Fig. 2 is the structure chart of Momordica grosvenori culture apparatus of the present invention.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification Word can be implemented according to this.
Embodiment 1
As Figure 1-Figure 2, a kind of ciltivating process of Momordica grosvenori, comprises the following steps:
Transplanting:The base portion of Momordica grosvenori rooted seedling after sterilization is coated with sponge, is transplanted into contains water planting battalion afterwards In the cultivation bed 2 of nutrient solution, the 1/2 of the root system of Momordica grosvenori rooted seedling is set to immerse in the mill water culture nutrient solution;
Wherein, be provided with matched lid 1 on the cultivation bed 2, the lid 1 be provided with a first through hole and Several apertures are 2cm the second through hole with the fixation Momordica grosvenori rooted seedling, and body, the pipe are inserted with the first through hole The outlet side of body and the root of the Momordica grosvenori rooted seedling are in same level;
Water planting management:The Momordica grosvenori is taken root after transplantation of seedlings, keep the mill water culture nutrient solution pH value be 5.5, daily to Air 30min is passed through in the body, when the root system that mill water culture nutrient solution submerges the Momordica grosvenori is no more than 1/3, then changes institute State mill water culture nutrient solution.
Wherein, the breeding method of the Momordica grosvenori rooted seedling is that the Momordica grosvenori that will be cultivated by subculture and leaf color is dark green is sterile Tissue culture plant inoculation is cultivated to taking root into root media, the methyl α-naphthyl acetate containing MS, 0.2mg/L in the root media, The sucrose of 0.1mg/L indolebutyric acid, 4.5g/L agar and 30g/L.
Wherein, the mill water culture nutrient solution per 10L contains following component:Calcium nitrate tetrahydrate 810mg, potassium nitrate 580mg, Ammonium dihydrogen phosphate 140mg, magnesium sulfate 365mg, disodium ethylene diamine tetraacetate iron 13mg, four hydrated manganese sulfate 0.6mg, boric acid 1.0mg, Salzburg vitriol 0.02mg, zinc sulfate 0.06mg and molybdic acid 0.01mg.
Wherein, the 10th day after transplanting, beer waste water nutrient solution is substituted for by the mill water culture nutrient solution, wherein, it is described Beer waste water nutrient solution by COD value less than or equal to 120mg/L beer waste water, acidifying bacterium and trace element be in mass ratio 1000: 1: 10 compositions.
Wherein, the cultivation bed 2 is glass material, and the cultivation bed 2 that transplanting has Momordica grosvenori rooted seedling is placed in into Momordica grosvenori plants Train in device, and magnetic stir bar is added in cultivation bed 2, wherein, the Momordica grosvenori culture apparatus includes light-passing board 4 and carrying The magnetic stirring apparatus 3 of the cultivation bed 2, the light-passing board 4 and magnetic stirring apparatus 3 are fixed by several support columns 6, described LED light source 5 is provided with light-passing board 4, after Momordica grosvenori takes root transplantation of seedlings, uses magnetic stir bar and magnetic stirring apparatus 3 daily Mill water culture nutrient solution in the cultivation bed 2 is replaced or beer waste water nutrient solution stirs 2 hours.
Wherein, the Momordica grosvenori rooted seedling after transplanting maintains the temperature at 22 DEG C, intensity of illumination 8200Lx.
Wherein, the sodium hydroxide that the reagent for adjusting pH is the salpeter solution that mass fraction is 5% or mass fraction is 5% Solution.
Wherein, the method for sterilizing Momordica grosvenori rooted seedling is that Momordica grosvenori rooted seedling is put into more bacterium that mass fraction is 0.1% Soaked 10 minutes in clever solution.
Embodiment 2
As Figure 1-Figure 2, a kind of ciltivating process of Momordica grosvenori, comprises the following steps:
Transplanting:The base portion of Momordica grosvenori rooted seedling after sterilization is coated with sponge, is transplanted into contains water planting battalion afterwards In the cultivation bed 2 of nutrient solution, the 5/6 of the root system of Momordica grosvenori rooted seedling is set to immerse in the mill water culture nutrient solution;
Wherein, be provided with matched lid 1 on the cultivation bed 2, the lid 1 be provided with a first through hole and Several apertures are 4cm the second through hole with the fixation Momordica grosvenori rooted seedling, and body, the pipe are inserted with the first through hole The outlet side of body and the root of the Momordica grosvenori rooted seedling are in same level;
Water planting management:The Momordica grosvenori is taken root after transplantation of seedlings, keep the mill water culture nutrient solution pH value be 6.5, daily to Air 30min is passed through in the body, when the root system that mill water culture nutrient solution submerges the Momordica grosvenori is no more than 1/3, then changes institute State mill water culture nutrient solution.
Wherein, the breeding method of the Momordica grosvenori rooted seedling is that the Momordica grosvenori that will be cultivated by subculture and leaf color is dark green is sterile Tissue culture plant inoculation is cultivated to taking root into root media, the methyl α-naphthyl acetate containing MS, 0.2mg/L in the root media, The sucrose of 0.1mg/L indolebutyric acid, 4.5g/L agar and 30g/L.
Wherein, the mill water culture nutrient solution per 10L contains following component:Calcium nitrate tetrahydrate 830mg, potassium nitrate 620mg, Ammonium dihydrogen phosphate 160mg, magnesium sulfate 375mg, disodium ethylene diamine tetraacetate iron 19mg, four hydrated manganese sulfate 0.8mg, boric acid 1.4mg, Salzburg vitriol 0.06mg, zinc sulfate 0.1mg and molybdic acid 0.01mg.
Wherein, the 15th day after transplanting, beer waste water nutrient solution is substituted for by the mill water culture nutrient solution, wherein, it is described Beer waste water nutrient solution by COD value less than or equal to 120mg/L beer waste water, acidifying bacterium and trace element be in mass ratio 1000: 5: 15 compositions.
Wherein, the cultivation bed 2 is glass material, and the cultivation bed 2 that transplanting has Momordica grosvenori rooted seedling is placed in into Momordica grosvenori plants Train in device, and magnetic stir bar is added in cultivation bed 2, wherein, the Momordica grosvenori culture apparatus includes light-passing board 4 and carrying The magnetic stirring apparatus 3 of the cultivation bed 2, the light-passing board 4 and magnetic stirring apparatus 3 are fixed by several support columns 6, described LED light source 5 is provided with light-passing board 4, after Momordica grosvenori takes root transplantation of seedlings, uses magnetic stir bar and magnetic stirring apparatus 3 daily Mill water culture nutrient solution in the cultivation bed 2 is replaced or beer waste water nutrient solution stirs 3 hours.
Wherein, the Momordica grosvenori rooted seedling after transplanting maintains the temperature at 25 DEG C, intensity of illumination 8500Lx.
Wherein, the sodium hydroxide that the reagent for adjusting pH is the salpeter solution that mass fraction is 5% or mass fraction is 5% Solution.
Wherein, the method for sterilizing Momordica grosvenori rooted seedling is that Momordica grosvenori rooted seedling is put into more bacterium that mass fraction is 0.1% Soaked 20 minutes in clever solution.
Embodiment 3
As Figure 1-Figure 2, a kind of ciltivating process of Momordica grosvenori, comprises the following steps:
Transplanting:The base portion of Momordica grosvenori rooted seedling after sterilization is coated with sponge, is transplanted into contains water planting battalion afterwards In the cultivation bed 2 of nutrient solution, the 1/3 of the root system of Momordica grosvenori rooted seedling is set to immerse in the mill water culture nutrient solution;
Wherein, be provided with matched lid 1 on the cultivation bed 2, the lid 1 be provided with a first through hole and Several apertures are 3cm the second through hole with the fixation Momordica grosvenori rooted seedling, and body, the pipe are inserted with the first through hole The outlet side of body and the root of the Momordica grosvenori rooted seedling are in same level;
Water planting management:The Momordica grosvenori is taken root after transplantation of seedlings, and the pH value for keeping the mill water culture nutrient solution is 6, daily to institute State and air 30min is passed through in body, when the root system that mill water culture nutrient solution submerges the Momordica grosvenori is no more than 1/3, then described in replacing Mill water culture nutrient solution.
Wherein, the breeding method of the Momordica grosvenori rooted seedling is that the Momordica grosvenori that will be cultivated by subculture and leaf color is dark green is sterile Tissue culture plant inoculation is cultivated to taking root into root media, the methyl α-naphthyl acetate containing MS, 0.2mg/L in the root media, The sucrose of 0.1mg/L indolebutyric acid, 4.5g/L agar and 30g/L.
Wherein, the mill water culture nutrient solution per 10L contains following component:Calcium nitrate tetrahydrate 820mg, potassium nitrate 600mg, Ammonium dihydrogen phosphate 150mg, magnesium sulfate 370mg, disodium ethylene diamine tetraacetate iron 16mg, four hydrated manganese sulfate 0.7mg, boric acid 1.2mg, Salzburg vitriol 0.04mg, zinc sulfate 0.08mg and molybdic acid 0.01mg.
Wherein, the 12nd day after transplanting, beer waste water nutrient solution is substituted for by the mill water culture nutrient solution, wherein, it is described Beer waste water nutrient solution by COD value less than or equal to 120mg/L beer waste water, acidifying bacterium and trace element be in mass ratio 1000: 3: 12 compositions.
Wherein, the cultivation bed 2 is glass material, and the cultivation bed 2 that transplanting has Momordica grosvenori rooted seedling is placed in into Momordica grosvenori plants Train in device, and magnetic stir bar is added in cultivation bed 2, wherein, the Momordica grosvenori culture apparatus includes light-passing board 4 and carrying The magnetic stirring apparatus 3 of the cultivation bed 2, the light-passing board 4 and magnetic stirring apparatus 3 are fixed by several support columns 6, described LED light source 5 is provided with light-passing board 4, after Momordica grosvenori takes root transplantation of seedlings, uses magnetic stir bar and magnetic stirring apparatus 3 daily Mill water culture nutrient solution in the cultivation bed 2 is replaced or beer waste water nutrient solution stirs 2.5 hours.
Wherein, the Momordica grosvenori rooted seedling after transplanting maintains the temperature at 23 DEG C, intensity of illumination 8300Lx.
Wherein, the sodium hydroxide that the reagent for adjusting pH is the salpeter solution that mass fraction is 5% or mass fraction is 5% Solution.
Wherein, the method for sterilizing Momordica grosvenori rooted seedling is that Momordica grosvenori rooted seedling is put into more bacterium that mass fraction is 0.1% Soaked 15 minutes in clever solution.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited In specific details and shown here as the legend with description.

Claims (5)

1. a kind of ciltivating process of Momordica grosvenori, it is characterised in that comprise the following steps:
Transplanting:The base portion of Momordica grosvenori rooted seedling after sterilization is coated with sponge, is transplanted into contains mill water culture nutrient solution afterwards Cultivation bed in, make the 1/2~5/6 of the root system of Momordica grosvenori rooted seedling to immerse in the mill water culture nutrient solution;
Wherein, matched lid is provided with the cultivation bed, the lid is provided with a first through hole and several holes Footpath is 2~4cm the second through hole with the fixation Momordica grosvenori rooted seedling, and body, the body are inserted with the first through hole Outlet side and the Momordica grosvenori rooted seedling root in same level;
Water planting management:The Momordica grosvenori is taken root after transplantation of seedlings, between the pH value for keeping the mill water culture nutrient solution is 5.5~6.5, often It is passed through air 30min into the body, when the root system that mill water culture nutrient solution submerges the Momordica grosvenori is no more than 1/3, then more Change the mill water culture nutrient solution;
The breeding method of the Momordica grosvenori rooted seedling is to connect the sterile tissue-cultured seedling of Momordica grosvenori cultivated by subculture and leaf color is dark green Kind into root media, cultivate to taking root, the methyl α-naphthyl acetate containing MS, 0.2mg/L in the root media, 0.1mg/L The sucrose of indolebutyric acid, 4.5g/L agar and 30g/L;
The mill water culture nutrient solution per 10L contains following component:810~830mg of calcium nitrate tetrahydrate, potassium nitrate 580~ 620mg, 140~160mg of ammonium dihydrogen phosphate, 365~375mg of magnesium sulfate, 13~19mg of disodium ethylene diamine tetraacetate iron, four hydrations 0.6~0.8mg of manganese sulfate, 1.0~1.4mg of boric acid, 0.02~0.06mg of Salzburg vitriol, 0.06~0.1mg of zinc sulfate with And molybdic acid 0.01mg;
The 10th~15 day after transplanting, the mill water culture nutrient solution is substituted for beer waste water nutrient solution, wherein, the beer gives up Water nutrition liquid is 1000 in mass ratio by beer waste water of the COD value less than or equal to 120mg/L, acidifying bacterium and trace element:1 ~5:10~15 compositions.
2. the ciltivating process of Momordica grosvenori as claimed in claim 1, it is characterised in that the cultivation bed is glass material, will be moved The cultivation bed that planting has Momordica grosvenori rooted seedling is placed in Momordica grosvenori culture apparatus, and magnetic stir bar is added in cultivation bed, wherein, The Momordica grosvenori culture apparatus includes light-passing board and carries the magnetic stirring apparatus of the cultivation bed, and the light-passing board and magnetic force stir Mix device to fix by several support columns, LED light source is provided with the light-passing board, after Momordica grosvenori takes root transplantation of seedlings, daily The mill water culture nutrient solution in the cultivation bed is replaced with magnetic stir bar and magnetic stirring apparatus or beer waste water nutrient solution stirs 2-3 hours.
3. the ciltivating process of Momordica grosvenori as claimed in claim 2, it is characterised in that the Momordica grosvenori rooted seedling after transplanting keeps temperature Degree is 8200~8500Lx in 22~25 DEG C, intensity of illumination.
4. the ciltivating process of Momordica grosvenori as claimed in claim 3, it is characterised in that the reagent for adjusting pH is that mass fraction is 5% salpeter solution or mass fraction is 5% sodium hydroxide solution.
5. the ciltivating process of Momordica grosvenori as claimed in claim 4, it is characterised in that the method for sterilization Momordica grosvenori rooted seedling is will Momordica grosvenori rooted seedling, which is put into the carbendazim solution that mass fraction is 0.1%, soaks 10~20 minutes.
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Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1217307A (en) * 1998-04-25 1999-05-26 阮芳恺 Plant foliage fertilizer using waste-liquid from beer-production as raw material
CN201499503U (en) * 2009-09-03 2010-06-09 北京中环易达设施园艺科技有限公司 Novel movable pipeline cultivation device
CN101828509B (en) * 2010-05-14 2012-02-29 福建农林大学 Water culture technique for anoectochilus formosanus
CN102276358B (en) * 2011-07-21 2015-10-28 长子县方兴现代农业有限公司 Soilless culture cherry tomato nutrient solution and preparation method
CN102617216B (en) * 2012-04-01 2014-04-23 广西壮族自治区药用植物园 Huperzia serrata cultivation nutrient solution
JP2013236560A (en) * 2012-05-11 2013-11-28 Sharp Corp Hydroponic culture container
CN202958372U (en) * 2012-09-18 2013-06-05 李文虎 Vegetable and flower soilless culture tank
CN103503756A (en) * 2013-10-23 2014-01-15 镇江市丹徒区紫杉生态农业园 Nutrient solution cultivation method for dracaena fragrans
CN104542278B (en) * 2014-12-22 2017-04-19 广西壮族自治区药用植物园 Cultivation method for polyploidy siraitia grosvenorii plant

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