CN101707980B - Sugarcane tissue culture seedling bagging outdoor rooting culture method - Google Patents
Sugarcane tissue culture seedling bagging outdoor rooting culture method Download PDFInfo
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- CN101707980B CN101707980B CN2009101146029A CN200910114602A CN101707980B CN 101707980 B CN101707980 B CN 101707980B CN 2009101146029 A CN2009101146029 A CN 2009101146029A CN 200910114602 A CN200910114602 A CN 200910114602A CN 101707980 B CN101707980 B CN 101707980B
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Abstract
The invention provides a sugarcane tissue culture seedling bagging outdoor rooting culture method, which is characterized by comprising the following steps: putting a sugarcane liquid rooting culture medium into a plastic film bag, sterilizing at high temperature, inoculating, sealing, and putting into a greenhouse, wherein the rooting culture and the seedling hardening process are carried out at the same time under the outdoor natural conditions; and moving the tissue culture seedlings out of the bag after rooting, and planting in groups. Compared with the prior method, the method of the invention does not occupy the space of the culture room during rooting culture, has the advantages of low pollution rate, favorable light acceptance, good quality of roots and seedlings and the like, can remove the seedlings out of the bag for group planting for 5 days in advance, enhances the transplanting survival percent by more than 20%, is convenient for long-distance transportation, and is suitable for mass sugarcane tissue culture seedling rooting culture.
Description
Technical field
The present invention relates to a kind of method of tissue culture, specifically a kind of sugarcane tissue-culture seedling bagging outdoor rooting cultured method.
Background technology
The application organizes culture technique is to accelerate the major technique means of new stock breeding promotion rate of sugarcane and the production of detoxification health seedling, and carries out large-scale application aborning.At present, to make fabric texture the same with other for sugarcane tissue-culture, most Pyrex flask, wide-mouth bottle, the triangular flasks etc. of adopting of culture vessel.The vial consumption is prone to loss greatly again, and especially it takes up room greatly, causes a large amount of wastes of facilities and equipment, and production cost is higher; In addition, need sufficient optical condition, the seedling of particularly taking root in the sugarcane tissue-culture seedling reproductive process; If optical condition is bad; The quality of its root and seedling a little less than, transplanting survival rate is low, improve the quality of root and seedling; Except that needs suitable culture base, otherwise effective technique measure is to prolong the seedling room of taking root to refine the seedling time outward.Adopt vial to carry out culture of rootage except that aforementioned deficiency at present; Also be unfavorable for organizing the refining seedling of training the seedling of taking root; Main performance has, and the one, if tissue cultivating seedling is taken root before, the bottle seedling is not shifted out culturing room's refining seedling of taking root, because the outdoor temperature variation causes lid to expand with heat and contract with cold more greatly; The inside and outside gas of blake bottle exists and exchanges phenomenon, and the air mushroom gets in the bottle and improves pollution rate greatly; The back is shifted out culturing room to the bottle seedling and is refined seedling if the group training is taken root; Because oxygen absorbs in the tissue cultivating seedling rooting process, consumes in most of nutrition of medium and the bottle; Nutritional condition is relatively poor in the bottle, and the refining seedling time is unsuitable long, otherwise the seedling that causes taking root is dead because of oligotrophy; If refining seedling deficiency of time then reduces refining seedling effect.
In addition, in group training process, also utilize sack production at other plants such as bananas, but its medium is a solid, production cost is higher.
Summary of the invention
The invention provides a kind of employing plastic film bag and carry out the outdoor rooting cultured method, do not take the culturing room space, improve the availability of facilities and equipment greatly, reduce production costs; Tissue cultivating seedling is in outdoor in rooting process all the time, and pollution rate is low, near natural conditions, receives light good, and root and seedling are more sturdy; Root media is a liquid, and cost is low, and nutrition is beneficial to tissue cultivating seedling and absorbs, and seedling quality is high.
The present invention realizes through following technical scheme:
With the sugarcane liquid root media plastic film bag of packing into, through autoclaving, booth is put in the sealing of inoculation back, and its culture of rootage is all carried out under outdoor natural conditions with refining seedling process simultaneously, is that extensible sack carries out clump and plants after tissue cultivating seedling is taken root.
Described sugarcane liquid root media is the liquid medium that does not contain agar, and its Main Ingredients and Appearance is MS+NAA5.0mg/L+ white sugar 50g/L, pH value 5.8.
Described plastic film bag is that wall thickness is 0.05mm～0.08mm, and wide 9～11 centimetres, high 11～13 centimetres, light permeable rate is 90～100 polypropylene film bag.
Described autoclaving is with the root media polypropylene film bag being housed, placing damp and hot autoclave sterilization container through 121～125 ℃, 20～30 minutes.
The sealing of described inoculation back, be on superclean bench prepare to carry out culture of rootage organize seedling to insert the medium temperature to have cooled off the sack with the room temperature basically identical, sealed with sealing machine then.
Described booth ventilates, scattered light foot, no direct sunlight, flashing, wind proofing.
Described culture of rootage and refining seedling are on the booth shelf, to become a row with connecting one in the seedling sack of taking root near a vertical pendulum, between every row's sack across about 5 centimetres; Be that extensible sack carries out clump and plants after tissue cultivating seedling is taken root.
Beneficial effect of the present invention
Adopt the present invention, save the culture of rootage chamber, improve propagation seedling culture space, improve production scale greatly; Culture medium cost is low, and the seedling quality of taking root is high, can shift to an earlier date 5～7 day time output clump and plant, and transplanting survival rate is high; Convenient long-distance transport improves productivity effect; Reduce production costs.And whole rooting process is accomplished under outdoor conditions.This method is compared with existing method to be had culture of rootage and not to take the culturing room space, and pollution rate is low, and root media is a liquid; Cost is low, and nutrition is beneficial to tissue cultivating seedling and absorbs, and Miao Shouguang is good; Quality is high, and transplanting survival rate improves more than 20%, is fit to sugarcane tissue-culture seedling rooting in enormous quantities and cultivates.
Embodiment
1. with liquid root media (the MS+NAA5.0mg/L+ white sugar 50g/L of 25～28ml; PH value 5.8) wall thickness of packing into is that 0.05mm～0.08mm, wide 9～11 centimetres, high 11～13 centimetres, light permeable rate are in 90%～100% the polypropylene film bag; Be one group about 10, clamp sack, put into special container (case or bag) then with clip; Put into the autoclave sterilizer sterilization, disinfecting time is 25～30 minutes under 120～125 ℃, 1.1 atmospheric pressure; It is subsequent use that the medium that disinfects is placed the cooling of aseptic culture chamber; (MS is that the most general a kind of medium title is used in Plant Tissue Breeding, and main component is the required number of chemical material of plant growing, its public use of filling a prescription; NAA is a kind of growth hormone analog, the Chinese naa, Shanghai is sowed rich garden supplies Co., Ltd and is provided)
2. above-mentioned special container (case or bag) can be the metal chest of air-vent or bigger bag film or paper bag;
3. after taking out packed medium on the superclean bench, insert and prepare the seedling of organizing of taking root, seal sack with sealing machine then, before the envelope mouth; Sack is opened; So that air content reaches higher level in the sack, if little air is unfavorable for that tissue cultivating seedling is taken root and growth; After sealing sack, whether the inspection sack leaks gas, to the sack of gas leakage to sack front cover time again.
4. the sack of sealing sack be positioned over ventilation well, the outdoor booth of scattered light foot, no direct sunlight, flashing, wind proofing carries out culture of rootage, the sack amount of putting vertical pendulum one by one becomes a row, between every row 5 centimetres at interval, helps printing opacity; Or place have rainproof, sunshade, the place of condition such as ventilation carries out outdoor rooting and cultivates.
5. the sugarcane tissue-culture seedling bagging outdoor rooting cultivates in the Nanning that Best Times is 3～June and 10～December in 1 year, and this time period temperature is higher, and illumination condition is preferably arranged, and it is fast and neat to go out root, and offspring quality is high; 1～February is overcast and rainy many, and temperature is low, and long root is slow, and with good conditionsi heating added lustre to; In 7～September, temperature is too high, and the tissue cultivating seedling growth is fast, is prone to aging, can improve ventilation condition, suitably cooling.
6. in process of rooting culture, be prone to the more globule that condenses in the sack, certain influence arranged receiving light; Simultaneously, because tissue cultivating seedling closely contacts with sack, than long dead seedling can appear if direct sunlight shines the solarization time.
7. daily mean temperature is more than 20 ℃ the time, and the sugarcane tissue-culture seedling is cultivated and begun long root in about 10 days, and root was long neat in 15～20 days, if indoor cultivation need carry out outdoor refining seedling time more than 5 days behind the neat root of bottle seedling length; And the present invention is taken root and the refining seedling carries out simultaneously, behind the long neat root directly output transplant the one-level seedbed, save the refining seedling time, and its offspring quality is good, its survival rate is higher than routine more than 20%.
Claims (6)
1. sugarcane tissue-culture seedling bagging outdoor rooting cultured method; It is characterized in that: with the sugarcane liquid root media of MS+NAA5.0mg/L+ white sugar 50g/L, pH value 5.8; The wall thickness of packing into is that 0.05mm～0.08mm, wide 9～11 centimetres, high 11～13 centimetres, light permeable rate are 90%～100% polypropylene film bag; Through autoclaving, booth is put in the sealing of inoculation back; Its culture of rootage is all carried out under outdoor natural conditions with refining seedling process simultaneously, is that extensible sack carries out clump and plants after tissue cultivating seedling is taken root.
2. according to the said sugarcane tissue-culture seedling bagging outdoor rooting of claim 1 cultured method, it is characterized in that: described sugarcane liquid root media is the liquid medium that does not contain agar.
3. according to the said sugarcane tissue-culture seedling bagging outdoor rooting of claim 1 cultured method, it is characterized in that: described autoclaving is with the polypropylene film bag that root media is housed, and places damp and hot autoclave sterilization container through 121～125 ℃, 20～30 minutes.
4. according to the said sugarcane tissue-culture seedling bagging outdoor rooting of claim 1 cultured method; It is characterized in that: described inoculation back sealing; Be on superclean bench prepare to carry out culture of rootage organize seedling to insert the medium temperature to have cooled off the sack with the room temperature basically identical, sealed with sealing machine then.
5. according to the said sugarcane tissue-culture seedling bagging outdoor rooting of claim 1 cultured method, it is characterized in that: described booth, ventilate, scattered light foot, no direct sunlight, flashing, wind proofing.
6. sugarcane tissue-culture seedling bagging outdoor rooting cultural method according to claim 1; It is characterized in that: described culture of rootage and refining seedling; Be to connect one in the seedling sack of taking root on the booth shelf, to become a row, be separated by 5 centimetres between every row's sack near a vertical pendulum; Be that extensible sack carries out clump and plants after tissue cultivating seedling is taken root.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101146029A CN101707980B (en) | 2009-11-30 | 2009-11-30 | Sugarcane tissue culture seedling bagging outdoor rooting culture method |
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| CN2009101146029A CN101707980B (en) | 2009-11-30 | 2009-11-30 | Sugarcane tissue culture seedling bagging outdoor rooting culture method |
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| CN101707980A CN101707980A (en) | 2010-05-19 |
| CN101707980B true CN101707980B (en) | 2012-04-25 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904262B (en) * | 2010-07-16 | 2012-02-15 | 广西壮族自治区甘蔗研究所 | Ex vitro rooting method of sugarcane test tube plantlets |
| CN102498912B (en) * | 2011-11-23 | 2014-07-23 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
| CN102907328B (en) * | 2012-11-14 | 2014-08-27 | 中国热带农业科学院热带生物技术研究所 | Method for utilizing microelements to accelerate tissue culture seedling of sugarcane |
| CN103430755A (en) * | 2013-09-06 | 2013-12-11 | 广西壮族自治区农业科学院甘蔗研究所 | Method for developing healthy sugarcane seeds |
| CN104920211B (en) * | 2015-05-26 | 2018-06-22 | 广西壮族自治区农业科学院甘蔗研究所 | It improves the take root growth of efficiency of sugarcane test tube seedling photoautotrophy and promotes liquid and method |
| CN109042215B (en) * | 2018-09-07 | 2020-12-25 | 广西生态工程职业技术学院 | Treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings |
| CN109287479A (en) * | 2018-10-15 | 2019-02-01 | 广西壮族自治区农业科学院甘蔗研究所 | The method that a kind of sugarcane health seedling field doubles breeding |
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