CN102884981A - Zanthoxylum nitidum tissue culture medium - Google Patents
Zanthoxylum nitidum tissue culture medium Download PDFInfo
- Publication number
- CN102884981A CN102884981A CN2012103649005A CN201210364900A CN102884981A CN 102884981 A CN102884981 A CN 102884981A CN 2012103649005 A CN2012103649005 A CN 2012103649005A CN 201210364900 A CN201210364900 A CN 201210364900A CN 102884981 A CN102884981 A CN 102884981A
- Authority
- CN
- China
- Prior art keywords
- medium
- subculture
- component
- rooting
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Hydroponics (AREA)
Abstract
The invention discloses a zanthoxylum nitidum tissue culture medium. The medium comprises a subculture medium and a rooting medium. Improvement is carried out on the basis of an H medium, the macroelement usage is adjusted, and the iron vitriol content is increased. The medium is suitable for culturing zanthoxylum nitidum and single zanthoxylum nitidum tissues from different producing areas. The subculture period takes 20-25 days, the growth coefficient is 4-6, subculture seedlings grow well, small seedlings are thick and order, the leaf color is green, no etiolated seedlings are produced, and the seedling yield is high. The rooting rate of the rooted seedlings can reach 90%, the rooting is quick; and when the temperature is at 25 plus or minus 2 DEG C, the average rooting time is 10-15 days, a root system well grows, the rooting number is larger and averagely 3-5 roots, the seedling is good in lignification, the transplanting survival rate reaches above 90%, and the outplanting time of bottled seedlings is about 30 days averagely.
Description
Technical field
The present invention relates to tissue culture medium (TCM), relate in particular to a kind of Radix zanthoxyli culture medium for tissue culture.
Background technology
Radix zanthoxyli (Zanthoxylum nitidum), be rutaceae, because all there is little hook thorn on its blade Zhong Mai two sides, so claim " Radix zanthoxyli ", have another name called two dorsal stylets, nail-plate thorn (Foochow), enter under mountain tiger, anaesthetic rattan, shinyleaf pricklyash root, the leaf to thread a needle, red barb muscle, Da Ye retouch pawl le (Guangdong, Guangxi).Radix zanthoxyli happiness is grown in warm, sun-drenched place, in the sparse woods on mountain region, hills, level land, the shrubbery, grass slope, deserted mountain have in the thornbush more commonly, mainly be distributed in the ground such as China Guangdong, Guangxi, Fujian, Hainan, Yunnan, Guizhou and Taiwan.Radix zanthoxyli is China's a kind of traditional Chinese medicine commonly used, and rhizome, leaf, pericarp all can be used as medicine, the effects such as the stasis of blood, analgesia, detumescence of invigorating blood circulation, fall apart.Modern medicinal chemical research shows: the rhizome of Radix zanthoxyli contains alkaloid oxynitidine, N-demethoxychelerythrone, dihydronitidine, skimmianine, 6-ethoxychelerythrine, α-allocryptopine, nitidine etc.During topical application, nerve endings is had anesthetic effect, and anaesthetic effect being stable, has no adverse reaction, also without hepatorenal damage etc., is a kind of traditional Chinese medicine with wide prospect in medicine, therefore carries out the basic research of Radix zanthoxyli, and tool is of great significance.
At present, domestic more existing Radix zanthoxyli tissue culture technology Primary Study reports, the general combination of adopting minimal medium (MS) and growth regulator in the selection of medium, but the Radix zanthoxyli provenance place of production is different, conditions of tissue culture is also different, even the Radix zanthoxyli provenance in the same place of production selects different individual plants to make explant, conditions of tissue culture is also different.The MS medium is applicable to part place of production Radix zanthoxyli tissue and cultivates, and part place of production Radix zanthoxyli provenance and Radix zanthoxyli individual plant carry out subculture with the MS medium to be cultivated, and first blade flavescence, bleaches, and then stem flavescence, bleaches, and the bud seedling is dead, can't proceed the subculture cultivation.If different places of production Radix zanthoxyli provenance or Radix zanthoxyli individual plant use a kind of medium, the production operation difficulty is large, and cost is high, is unfavorable for large-scale production.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of Radix zanthoxyli culture medium for tissue culture is provided, this medium comprises subculture medium and root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, disodium ethylene diamine tetraacetate 56.0mg/L, ferrous sulfate heptahydrate 41.7mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: 6-benzyladenine 0.4～1.0mg/L, methyl α-naphthyl acetate 0.2～0.5mg/L;
Surplus is distilled water.
The component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 33.4mg/L, disodium ethylene diamine tetraacetate 44.8mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: root-inducing powder ABT6 number 0.4～0.8mg/L, methyl α-naphthyl acetate 0.2～0.4mg/L;
Surplus is distilled water.
Subculture in the Radix zanthoxyli culture medium for tissue culture of the present invention, the compound method of root media are:
The preparation of 1 mother liquor
1.1 for ease of sampling, should prepare first various mother liquors, be divided into macroelement mother liquor, micro-mother liquor, organic matter mother liquor and each plant growth regulators mother liquor.Sucrose, agar should not be made into mother liquor, directly claim sample when needing;
1.2 each concentration of component of macroelement mother liquor and organic matter mother liquor should become with the concentration of component of medium 10～100 multiple relation, micro-mother liquor becomes 200～500 multiple relation, and the concentration of plant-growing-help chemicals mother liquor should be made into 1mg/ml;
1.3 mother liquor is selected aseptic distilled water, deionized water or ultra-pure water preparation, uses the water that boiled when producing in a large number;
1.4 during preparation macroelement mother liquor, each component should be dissolved separately, mixes one by one by the order of nitrogen, calcium, magnesium, phosphorus afterwards, otherwise causes precipitation easily;
1.5 micro-mother liquor contains the ferrous sulfate of seeing the labile potassium iodide of light and easy oxidation, uses brown bottle and preserves;
1.6 plant growth regulator mother liquor and organic matter mother liquor should place refrigerator to preserve;
1.7 should in time use after the mother liquor preparation, period of storage should not be above 1 month;
1.8 find that mother liquor has precipitation, or growth of microorganism arranged, or algal grown is arranged, should pass into disuse.
The preparation of 2 medium
2.1 according to culture medium prescription, measure in proportion mother liquor, go in the containers such as stainless-steel pan after adding the water constant volume;
2.2 add an amount of coagulating agent and sugar, and stir coagulating agent is fully disperseed, sugar fully dissolves;
2.3 be heated to firm boiling, coagulating agent dissolved fully.If when the self-reacting device that adopts the limit to divide rim to stir is prepared medium, can save the process that heating makes the coagulating agent dissolving;
2.4 be adjusted to suitable acid-base value with 1.0N hydrochloric acid or 1.0N sodium hydroxide;
Install in the culture vessel 2.5 as early as possible medium is divided, in order to avoid culture medium solidifying or retrogradation and be difficult to packing;
2.6 should avoid medium to adhere to bottleneck during the packing medium, in case be stained with, should wipe totally, otherwise cause easily pollution.
The sterilization of 3 medium
Put into the autoclave sterilizer sterilization 3.1 will divide the medium that installs;
3.2 the heating initial stage when the air pressure of sterilizing pan disinfection room reaches 0.05Mpa, is opened condensation trap, drains cold air in the disinfection room;
3.3 when the disinfection room internal gas pressure reaches 0.11Mpa, when temperature reaches 121 ℃, begins timing, keep temperature and pressure sterilization 15～20 minutes;
3.4 close the heating power supply switch, by slow exhaust mode discharging hot gas, when being down to atmospheric pressure, opens indoor air pressure to be sterilized sterilization pot cover or door, take out medium;
3.5 medium should place little air to flow and the clean environment cooling of few dust, otherwise causes mycotic spore to enter culture vessel in the cooling intake process, produces mould contamination.Or the thick cloth parcel cooling of sterilizing with high pressure-temperature.
4 medium storage
4.1 medium should be as far as possible now with the current.
4.2 can be at air clean and immobilising environment short time storage medium, should pass into disuse but storage surpasses 1 month medium.
The method that Radix zanthoxyli tissue of the present invention is cultivated is:
1. spore induction
In fine day choose robust growth, without the Radix zanthoxyli plant of damage by disease and insect, the young shoot of clip semi-lignified is as explant, cut off blade (staying petiole), clean 1～2 time with the water that is added with a little liquid detergent, rinse well with flowing water again, then at superclean bench with 0.1% mercury chloride sterilization 6-7 minute and shake for several times, use at last sterile water wash 4～5 times, the material that has disinfected is sheared the long section into about 2cm, every section is stayed 1～2 axillalry bud, then oblique cutting or vertically be inserted in the medium of induced bud.When explant is cultivated for the first time, need 10～15 days axillalry buds of complete dark cultivation can begin to sprout, and then move to the place cultivation of seeing light.
2. the propagation of bud is cultivated
Sprout when growing to the 1cm left and right sides when axillalry bud, cut axillalry bud and induce the differentiation Multiple Buds can carry out the shoot proliferation of bud, general one month subculture once, the subculture material all is placed on indoor cultivation, culturing room's temperature is (26 ± 2) ℃, illumination 12h.d
-1, illuminance 2000～3000lx.
3. what take root induces
When Multiple Buds grows to the 3cm left and right sides, can choose healthy and strong bud and transfer in the root media, carry out root induction, all the other bud clumps can be transferred to and allow it continue propagation in the subculture medium of newly joining.After budling changes in the root media, cultivating about 15 days under the indoor weak light condition first, root of hair rate (the low indoor cultivation of winter temperature is about 20 days, and the root of hair rate reaches more than 70%) to 50% time is moved outdoor hardening booth in natural scattering illumination cultivation 10～20 days (time autumn and winter is slightly long).
4. the take root transplanting of seedling
The abundant lignification of seedling to be taken root, blade is unfolded, and the leaf look dark green, and the seedling stem is dark green, the caulom elongation, height of seedling 4cm can transplant during the left and right sides.Pour out medium during transplanting, take out seedling, wash the medium that remains on the root, and clip long root system, it is long to keep 2～2.5cm, transplants in the nutritious bag of sterilizing through 0.2% potassium permanganate, the water of drenching, cover film keeps certain temperature and humidity, and will avoid direct sunlight.
Advantage of the present invention is:
1, the Radix zanthoxyli provenance place of production is different with individual plant, and conditions of tissue culture is also different, and the MS medium is applicable to part place of production Radix zanthoxyli tissue and cultivates, and the present invention is more suitable for the Radix zanthoxyli in the different places of production and the tissue of different Radix zanthoxyli individual plants is cultivated.
2, part place of production Radix zanthoxyli provenance is carried out subculture with the MS medium and is cultivated, and first blade flavescence, bleaches, and then stem flavescence, bleaches, and can't proceed subculture and cultivate; With medium of the present invention Radix zanthoxyli is cultivated, adjusted the macroelement consumption, increased the content of ferrous sulfate heptahydrate, subculture cycle 20～25 days, growth coefficient 4～6, subculture seedling well-grown, seedling is sturdy, neat, the leaf look dark green, etiolated seedling (the gradually flavescence of blade, cauline leaf) few, and it is high to get the seedling rate.
3, in root media, increase the content of ferrous sulfate heptahydrate, cultivate with root media that the seedling rooting rate of taking root can reach 90%, to go out root fast, during 25 ± 2 ℃ of temperature, on average going out the root time is 10～15 days, well developed root system, and how go out the root amount on average has 3～5 roots, the seedling lignification is good, transplanting survival rate is high, and transplanting survival rate reaches more than 90%, and it is about 30 days that the bottle seedling goes out the garden time average.
Compare with the Radix zanthoxyli tissue culture medium (TCM) of present prior art, advantage of the present invention is:
Embodiment
The invention will be further described with embodiment for the below, but the present invention is not limited to these embodiment.
Embodiment 1:
The Radix zanthoxyli culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, disodium ethylene diamine tetraacetate 56.0mg/L, ferrous sulfate heptahydrate 41.7mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: 6-benzyladenine 0.4mg/L, methyl α-naphthyl acetate 0.2mg/L;
Surplus is distilled water.
The component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 33.4mg/L, disodium ethylene diamine tetraacetate 44.8mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: root-inducing powder ABT6 0.4mg/L, methyl α-naphthyl acetate 0.2mg/L;
Surplus is distilled water.
Embodiment 2:
The Radix zanthoxyli culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, disodium ethylene diamine tetraacetate 56.0mg/L, ferrous sulfate heptahydrate 41.7mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: 6-benzyladenine 1.0mg/L, methyl α-naphthyl acetate 0.5mg/L;
Surplus is distilled water.
The component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 33.4mg/L, disodium ethylene diamine tetraacetate 44.8mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: root-inducing powder ABT6 0.8mg/L, methyl α-naphthyl acetate 0.4mg/L;
Surplus is distilled water.
Embodiment 3:
The Radix zanthoxyli culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, disodium ethylene diamine tetraacetate 56.0mg/L, ferrous sulfate heptahydrate 41.7mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: 6-benzyladenine 0.6mg/L, methyl α-naphthyl acetate 0.3mg/L;
Surplus is distilled water.
The component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 33.4mg/L, disodium ethylene diamine tetraacetate 44.8mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: root-inducing powder ABT6 0.6mg/L, methyl α-naphthyl acetate 0.3mg/L;
Surplus is distilled water.
Embodiment 4:
The Radix zanthoxyli culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, disodium ethylene diamine tetraacetate 56.0mg/L, ferrous sulfate heptahydrate 41.7mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: 6-benzyladenine 1.0mg/L, methyl α-naphthyl acetate 0.2mg/L;
Surplus is distilled water.
The component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 33.4mg/L, disodium ethylene diamine tetraacetate 44.8mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.5mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, folic acid 0.5mg/L, vitamin h (VH) 0.05mg/L;
(4) plant growth regulator: root-inducing powder ABT6 0.6mg/L, methyl α-naphthyl acetate 0.4mg/L;
Surplus is distilled water.
Comparative Examples:
Subculture medium MS+6-BA2.0+NAA1.0, root media 1/2MS+ABT
10.8+IBA0.6 concentration unit is mg/L.
The medium that utilizes above-described embodiment 1 and Comparative Examples to make is organized cultivation to Radix zanthoxyli, can find out from the result of following table, and the technical indicator of embodiment far surpasses Comparative Examples.
Claims (2)
1. Radix zanthoxyli culture medium for tissue culture, this medium comprises subculture medium and root media, it is characterized in that:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, disodium ethylene diamine tetraacetate 56.0mg/L, ferrous sulfate heptahydrate 41.7mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.5mg/L, puridoxine hydrochloride 0.5mg/L, folic acid 0.5mg/L, vitamin h 0.05mg/L;
(4) plant growth regulator: 6-benzyladenine 0.4-1.0mg/L, methyl α-naphthyl acetate 0.2-0.5mg/L;
Surplus is distilled water.
2. Radix zanthoxyli culture medium for tissue culture according to claim 1 is characterized in that:
The component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1250mg/L, ammonium nitrate 1000mg/L, epsom salt 370mg/L, calcium chloride dihydrate 166mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 33.4mg/L, disodium ethylene diamine tetraacetate 44.8mg/L;
(2) trace element: four water manganese sulphate 25mg/L, white vitriol 10mg/L, boric acid 10mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassium iodide 0.083mg/L, cupric sulfate pentahydrate 0.025mg/L;
(3) organic matter: inositol 100.0mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.5mg/L, puridoxine hydrochloride 0.5mg/L, folic acid 0.5mg/L, vitamin h 0.05mg/L;
(4) plant growth regulator: root-inducing powder ABT6 0.4-0.8mg/L, methyl α-naphthyl acetate 0.2-0.4mg/L;
Surplus is distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210364900 CN102884981B (en) | 2012-09-26 | 2012-09-26 | Zanthoxylum nitidum tissue culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210364900 CN102884981B (en) | 2012-09-26 | 2012-09-26 | Zanthoxylum nitidum tissue culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102884981A true CN102884981A (en) | 2013-01-23 |
| CN102884981B CN102884981B (en) | 2013-07-24 |
Family
ID=47528838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210364900 Expired - Fee Related CN102884981B (en) | 2012-09-26 | 2012-09-26 | Zanthoxylum nitidum tissue culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102884981B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651148A (en) * | 2013-12-31 | 2014-03-26 | 茂名市天一农业科技发展有限公司 | Tissue culturing method of radix zanthoxyli |
| CN106508683A (en) * | 2016-11-24 | 2017-03-22 | 广州中医药大学 | A kind of tissue culture culture medium of Radix Zanthoxyli regrowth and cultural method |
| CN106718879A (en) * | 2016-11-24 | 2017-05-31 | 华南农业大学 | A kind of inducing culture of Radix zanthoxyli callus and abductive approach and application |
| CN106900555A (en) * | 2017-03-21 | 2017-06-30 | 钦州市林业科学研究所 | Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method |
| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
| CN112400695A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Culture medium for culturing evergreen common summer pink |
| CN112400696A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Tissue culture method of evergreen common selfheal fruit-spike bamboo |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099433A (en) * | 2007-08-06 | 2008-01-09 | 柳州两面针股份有限公司 | Reproduction method of plant Zanthoxylum Nitidum |
-
2012
- 2012-09-26 CN CN 201210364900 patent/CN102884981B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099433A (en) * | 2007-08-06 | 2008-01-09 | 柳州两面针股份有限公司 | Reproduction method of plant Zanthoxylum Nitidum |
Non-Patent Citations (4)
| Title |
|---|
| 《植物生理学通讯》 20060228 韦大器等 "两面针的组织培养和快速繁殖" 第73页 1-2 第42卷, 第1期 * |
| 《玉林师范学院学报（自然科学）》 20051231 王小敏等 "两面针的组织培养与快速繁殖" 第70-73页 1-2 第26卷, 第5期 * |
| 王小敏等: ""两面针的组织培养与快速繁殖"", 《玉林师范学院学报（自然科学）》 * |
| 韦大器等: ""两面针的组织培养和快速繁殖"", 《植物生理学通讯》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651148A (en) * | 2013-12-31 | 2014-03-26 | 茂名市天一农业科技发展有限公司 | Tissue culturing method of radix zanthoxyli |
| CN103651148B (en) * | 2013-12-31 | 2015-09-23 | 茂名市天一农业科技发展有限公司 | The method for tissue culture of a kind of Radix zanthoxyli |
| CN106508683A (en) * | 2016-11-24 | 2017-03-22 | 广州中医药大学 | A kind of tissue culture culture medium of Radix Zanthoxyli regrowth and cultural method |
| CN106718879A (en) * | 2016-11-24 | 2017-05-31 | 华南农业大学 | A kind of inducing culture of Radix zanthoxyli callus and abductive approach and application |
| CN106508683B (en) * | 2016-11-24 | 2018-03-20 | 广州中医药大学 | The tissue culture culture medium and cultural method of a kind of Radix zanthoxyli regrowth |
| CN106900555A (en) * | 2017-03-21 | 2017-06-30 | 钦州市林业科学研究所 | Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method |
| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
| CN112400695A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Culture medium for culturing evergreen common summer pink |
| CN112400696A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Tissue culture method of evergreen common selfheal fruit-spike bamboo |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102884981B (en) | 2013-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102884981B (en) | Zanthoxylum nitidum tissue culture medium | |
| CN103651121B (en) | A kind of bletilla differentiation, strong seedling culture base | |
| CN105010109B (en) | The ciltivating process of Momordica grosvenori | |
| CN104082138B (en) | A kind of tissue cultivation rapid breeding method of Radix Aristolochiae Tagalae | |
| CN104585035B (en) | A kind of method obtaining threeleaf akebia aseptic seedling | |
| CN101946709B (en) | Seedling raising method of plateau rhodiola crenulata | |
| CN103798145A (en) | Culture medium for tissue culture of vernonia amygdalina del. | |
| CN107047319B (en) | A kind of method for tissue culture and root media of ginkgo | |
| CN103880532A (en) | Hydroponic growing method of anoectochilus roburghii | |
| CN102823503B (en) | Tissue culture medium for propagating anthurium buds by using buds | |
| CN103798144A (en) | Culture medium for tissue culture of toona ciliate roem. | |
| CN103688865A (en) | Inducing medium and method for improving survival rate of butterfly orchid pedicel | |
| CN101622955B (en) | Culture medium composition suitable for germ-free germination of orchid seeds and method thereof | |
| CN104938184A (en) | Method for cultivating pakchoi which is highly rich in vitamin C | |
| CN102668853A (en) | Blueberry twig plant cuttage root-taking cultivation method | |
| CN103782912B (en) | Culture medium for red cassia tree tissue culture | |
| CN102668990A (en) | Plant tissue culturing method and device | |
| CN105850729A (en) | Verbena bonariensis cultivation method | |
| CN110089407B (en) | Bagging planting and storing method for anoectochilus roxburghii germplasm resources | |
| CN104429974A (en) | Rooting culture medium for culturing dendrobium officinale plantlets | |
| CN110214692A (en) | A kind of non-solid tissue culture culture medium of plant and the non-solid tissue culture method of plant | |
| CN103392594A (en) | Draceana arborea tissue culture seedling rooting induction technology | |
| CN106106083A (en) | A kind of preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) microsphere stem | |
| CN103858764B (en) | Centipeda minima tissue culture rapid propagation medium and centipeda minima one-time seedling formation tissue culture rapid propagation method | |
| CN102805032B (en) | Method for preventing daemonorops margaritae callus browning phenomena from occurring |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130724 Termination date: 20160926 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |