CN103880532A - Hydroponic growing method of anoectochilus roburghii - Google Patents
Hydroponic growing method of anoectochilus roburghii Download PDFInfo
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Abstract
The invention discloses a hydroponic growing method of anoectochilus roburghii. The hydroponic growing method comprises the steps of washing anoectochilus roburghii tissue culture bottle seedlings; transferring to a hydroponic device for culturing; placing the anoectochilus roburghii tissue culture seedlings into a culture bed on the hydroponic device; and only soaking roots into a culture solution, wherein hydroponic growing is divided into two stages: in the first stage, the anoectochilus roburghii is darkly cultured for 15 days at the room temperature of 27 +/-2 DEG C and the humidity of 80%-90%; in the second stage: the anoectochilus roburghii is brightly cultured for 75 days at the room temperature of 27 +/-2 DEG C and the humidity of 80%-90%, the illumination of the anoectochilus roburghii lasts for 9 hours per day and the illumination intensity is 1,700-2,000 Lux. By using the hydroponic growing method, the survival rate can be increased, and the culturing time can be shortened so as to realize industrial planting.
Description
Technical field
The present invention relates to biological technical field, refer in particular to a kind of water planting implantation methods of Anoectochilus roxburghii.
Background technology
Herba Anoectochili roxburghii (Anoectochilus roxburghii (Wall.) Lindl) is the per nnial herb that the orchid family is opened lip Cymbidium, is China tradition valuable ingredient of traditional Chinese medicine, has clearing heat and detoxicating, expelling wind and removing dampness, the effect such as nourishing Yin and moistening lung and hypotensive.Meanwhile, Herba Anoectochili roxburghii plant type is small and exquisite attractive in appearance, leaf grace, and vein golden yellow, is netted arrangement, has high ornamental value.
In prior art, Anoectochilus roxburghii tissue cultured seedling artificial growth is mainly that its defect is taking peat soil, river sand etc. as main planting matrix, not only implantation time is long, and biomass increases slow, and is prone to disease and pest, surviving rate is low, makes Anoectochilus roxburghii can not get very fast development.
Matrix plantation and water planting plantation are two kinds of methods common in plant growing, Anoectochilus roxburghii tissue cultured seedling is planted by water culture and artificial environment control, plant can absorb nutrient more fully, uniformly, increase biomass, reduce disease and pest, improve surviving rate, shorten incubation time, the advantage larger than having with matrix plantation, in view of this, this case produces thus.
Summary of the invention
The object of the present invention is to provide a kind of water planting implantation methods of Anoectochilus roxburghii, to improve surviving rate, shorten incubation time, thereby realize industrialization plantation.
For reaching above-mentioned purpose, solution of the present invention is:
A water planting implantation methods for Anoectochilus roxburghii, gets Anoectochilus roxburghii tissue-culture container seedling, cleans up, and moves to hydroponic device and cultivates, and the tissue cultured seedling of Herba Anoectochili roxburghii is placed in the cultivation bed on hydroponic device, and only root soaks in nutrient solution;
Water planting plantation is divided into two stages, dark cultivation 15 days of first stage, 27 ± 2 DEG C of room temps, humidity 80%-90%, nutrient composition is: saltpetre 113-148 mg/L, ammonium nitrate 62-85 mg/L, potassium primary phosphate 60-80 mg/L, magnesium sulfate heptahydrate 110-165 mg/L, four water-calcium nitrate 230-320 mg/L, potassiumiodide 0.05-0.08 mg/L, boric acid 2.2-3.0 mg/L, four water manganous sulfate 1.2-1.8 mg/L, Zinc Sulphate Heptahydrate 0.02-0.04 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.04 mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 12.0-16.4mg/L, iron vitriol 8.9-12.2mg/L, pH value 5.9,
Subordinate phase has light to cultivate 75 days, 27 ± 2 DEG C of room temps, humidity 80%-90%, illumination every day 9h, intensity is 1700-2000 Lux, nutrient composition is: saltpetre 122-183 mg/L, ammonium nitrate 82-98 mg/L, potassium primary phosphate 60-90 mg/L, magnesium sulfate heptahydrate 108-162 mg/L, four water-calcium nitrate 227-340 mg/L, potassiumiodide 0.06-0.08 mg/L, boric acid 2.3-3.5 mg/L, four water manganous sulfate 1.3-1.9 mg/L, Zinc Sulphate Heptahydrate 0.02-0.04 mg/L, Sodium orthomolybdate 0.02-0.04 mg/L, cupric sulfate pentahydrate 0.03-0.06mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 11.9-17.8mg/L, iron vitriol 8.8-13.3 mg/L, ironic citrate 14.3-21.5 mg/L, pH value 5.9.
The described first stage, the dark nutrient composition of cultivating was: saltpetre 123 mg/L, ammonium nitrate 71 mg/L, potassium primary phosphate 75 mg/L, magnesium sulfate heptahydrate 130 mg/L, four water-calcium nitrate 284 mg/L, potassiumiodide 0.07 mg/L, boric acid 2.9 mg/L, four water manganous sulfate 1.6 mg/L, Zinc Sulphate Heptahydrate 0.03 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.04 mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 13.4 mg/L, iron vitriol 10.0 mg/L, pH value 5.9.
Described subordinate phase has light to cultivate nutrient composition: saltpetre 153 mg/L, ammonium nitrate 82 mg/L, potassium primary phosphate 75 mg/L, magnesium sulfate heptahydrate 135 mg/L, four water-calcium nitrate 284 mg/L, potassiumiodide 0.07 mg/L, boric acid 2.9 mg/L, four water manganous sulfate 1.6 mg/L, Zinc Sulphate Heptahydrate 0.03 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.06mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 14.9 mg/L, iron vitriol 11.1 mg/L, ironic citrate 17.9 mg/L, pH value 5.9.
The described first stage is dark cultivates and subordinate phase has light to cultivate in the time of preparation molysite, and after disodium ethylene diamine tetraacetate, two kinds of compounds of iron vitriol are dissolved respectively, remix is 80 DEG C of water-bath chelatings 30 minutes, then adds in nutrient solution.
The described first stage is dark cultivates and subordinate phase has light to use phosphoric acid to determine pH value in cultivating.
Adopt after such scheme, water planting implantation methods of the present invention has solved Anoectochilus roxburghii tissue cultured seedling and has adopted ordinary method implantation time long, and biomass increases slow, be prone to disease and pest, the problems such as surviving rate is low, production cost height, have realized industrialization to plant fast, after 90 days, Herba Anoectochili roxburghii center line average is 13.9cm, increase 8.5cm, average strain weight reaches 2.6g, weightening finish 1.5g, survival rate of plant 100%, has met the demand that Anoectochilus roxburghii industrialization is produced.
Embodiment
Use the present invention to carry out the specific embodiment of the water planting plantation of Anoectochilus roxburghii tissue cultured seedling:
Get Anoectochilus roxburghii tissue-culture container seedling, clean up, move to hydroponic device and cultivate, the tissue cultured seedling of Herba Anoectochili roxburghii is placed in the cultivation bed on hydroponic device, and only root soaks in nutrient solution; Culture apparatus adopts NFT(nutrient film technology) formula solution culture facilities, this device is made up of nutrition liquid bath, cultivation bed, liquid feeding system, drainage system and the recycle system five parts, realizes the systemic circulation of nutritive medium.
Water planting plantation is divided into two stages, dark cultivation 15 days of first stage, 27 ± 2 DEG C of room temps, humidity 80%-90%, nutrient composition is: saltpetre 113-148 mg/L, ammonium nitrate 62-85 mg/L, potassium primary phosphate 60-80 mg/L, magnesium sulfate heptahydrate 110-165 mg/L, four water-calcium nitrate 230-320 mg/L, potassiumiodide 0.05-0.08 mg/L, boric acid 2.2-3.0 mg/L, four water manganous sulfate 1.2-1.8 mg/L, Zinc Sulphate Heptahydrate 0.02-0.04 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.04 mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 12.0-16.4mg/L, iron vitriol 8.9-12.2mg/L, pH value 5.9, use phosphoric acid to determine pH value.
Preparation nutritive medium uses pure water, and impurity in pure water, germ are few, and nutrient composition is stable.After preparation is dissolved disodium ethylene diamine tetraacetate, two kinds of compounds of iron vitriol respectively when molysite, remix is 80 DEG C of water-bath chelatings 30 minutes, then adds in nutrient solution.
The impact of different dark nutrient solutions on Herba Anoectochili roxburghii
Get Herba Anoectochili roxburghii tissue cultured seedling, center line average is 5.2cm, and weight in average is 1.1g, and 2.5 of average root of hair numbers, do 4 groups of experiments.Regulating culturing room's temperature is 27 ± 2 DEG C, and humidity is 80%-90%, cultivates 15 days.Component 1 height of seedling 6.7 cm, the average strain 1.3g that weighs, 3.6 of average root of hair numbers; Survival rate of plant reaches 100%, has no any yellow leaf and bad root phenomenon, is better than other components, the results are shown in Table 1.
The impact of the different dark nutrient solutions of table 1 on Herba Anoectochili roxburghii
? | Component 1 | Component 2 | Component 3 | Component 4 |
Saltpetre (mg/L) | 123 | 120 | 113 | 148 |
Ammonium nitrate (mg/L) | 71 | 68 | 62 | 85 |
Potassium primary phosphate (mg/L) | 75 | 70 | 60 | 80 |
Magnesium sulfate heptahydrate (mg/L) | 130 | 121 | 110 | 165 |
Four water-calcium nitrate (mg/L) | 284 | 261 | 230 | 320 |
Potassiumiodide (mg/L) | 0.07 | 0.06 | 0.05 | 0.08 |
Boric acid (mg/L) | 2.9 | 2.5 | 2.2 | 3.0 |
Four water manganous sulfates (mg/L) | 1.6 | 1.5 | 1.2 | 1.8 |
Zinc Sulphate Heptahydrate (mg/L) | 0.03 | 0.03 | 0.02 | 0.04 |
Sodium orthomolybdate (mg/L) | 0.03 | 0.03 | 0.03 | 0.03 |
Cupric sulfate pentahydrate (mg/L) | 0.04 | 0.04 | 0.04 | 0.04 |
Cobalt chloride (mg/L) | 0.01 | 0.01 | 0.01 | 0.01 |
Disodium ethylene diamine tetraacetate (mg/L) | 13.4 | 14.9 | 12.0 | 16.4 |
Iron vitriol (mg/L) | 10.0 | 11.1 | 8.9 | 12.2 |
Use phosphoric acid definite value PH | 5.9 | 5.9 | 5.9 | 5.9 |
Center line average (cm) | 6.7 | 6.5 | 6.1 | 6.0 |
Weight in average (g) | 1.3 | 1.05 | 1.13 | 1.2 |
Mean elements (bar) | 3.6 | 3.2 | 2.9 | 2.8 |
Subordinate phase has light to cultivate 75 days, 27 ± 2 DEG C of room temps, humidity 80%-90%, illumination every day 9h, intensity is 1700-2000 Lux, nutrient composition is: saltpetre 122-183 mg/L, ammonium nitrate 82-98 mg/L, potassium primary phosphate 60-90 mg/L, magnesium sulfate heptahydrate 108-162 mg/L, four water-calcium nitrate 227-340 mg/L, potassiumiodide 0.06-0.08 mg/L, boric acid 2.3-3.5 mg/L, four water manganous sulfate 1.3-1.9 mg/L, Zinc Sulphate Heptahydrate 0.02-0.04 mg/L, Sodium orthomolybdate 0.02-0.04 mg/L, cupric sulfate pentahydrate 0.03-0.06mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 11.9-17.8mg/L, iron vitriol 8.8-13.3 mg/L, ironic citrate 14.3-21.5 mg/L, pH value 5.9, use phosphoric acid to determine pH value.
Preparation nutritive medium uses pure water, and impurity in pure water, germ are few, and nutrient composition is stable.After preparation is dissolved disodium ethylene diamine tetraacetate, two kinds of compounds of iron vitriol respectively when molysite, remix is 80 DEG C of water-bath chelatings 30 minutes, then adds in nutrient solution.
The impact of different light nutrient solution on Herba Anoectochili roxburghii
Get Herba Anoectochili roxburghii tissue cultured seedling, center line average is 6.7cm, and weight in average is 1.3g, and 3.6 of average root of hair numbers, do 4 groups of experiments.Regulating culturing room's temperature is 27 ± 2 DEG C, and humidity is 80%-90%, illumination every day 9h, and intensity is 1700~2000 Lux, cultivates 75 days.Component 1 height of seedling 13.9 cm, the average strain 2.6g that weighs, survival rate of plant reaches 100%, and robust plant has no any yellow leaf and bad root phenomenon, is better than other components. the results are shown in Table 2.
The impact of table 2 different light nutrient solution on Herba Anoectochili roxburghii
Composition | Component 1 | Component 2 | Component 3 | Component 4 |
Saltpetre (mg/L) | 153 | 153 | 183 | 122 |
Ammonium nitrate (mg/L) | 82 | 82 | 98 | 67 |
Potassium primary phosphate (mg/L) | 75 | 75 | 90 | 60 |
Magnesium sulfate heptahydrate (mg/L) | 135 | 135 | 162 | 108 |
Four water-calcium nitrate (mg/L) | 284 | 284 | 340 | 227 |
Potassiumiodide (mg/L) | 0.07 | 0.07 | 0.08 | 0.06 |
Boric acid (mg/L) | 2.9 | 2.9 | 3.5 | 2.3 |
Four water manganous sulfates (mg/L) | 1.6 | 1.6 | 1.9 | 1.3 |
Zinc Sulphate Heptahydrate (mg/L) | 0.03 | 0.03 | 0.04 | 0.02 |
Sodium orthomolybdate (mg/L) | 0.03 | 0.03 | 0.04 | 0.02 |
Cupric sulfate pentahydrate (mg/L) | 0.06 | 0.06 | 0.05 | 0.03 |
Cobalt chloride (mg/L) | 0.01 | 0.01 | 0.01 | 0.01 |
Disodium ethylene diamine tetraacetate (mg/L) | 14.9 | 14.9 | 17.8 | 11.9 |
Iron vitriol (mg/L) | 11.1 | 11.1 | 13.3 | 8.8 |
Ironic citrate (mg/L) | 17.9 | 0 | 21.5 | 14.3 |
Use phosphoric acid definite value PH | 5.9 | 5.9 | 5.9 | 5.9 |
Center line average (cm) | 13.9 | 13.5 | 12.8 | 12.4 |
Weight in average (g) | 2.6 | 2.2 | 2.1 | 2.3 |
Surviving rate (%) | 100 | 100 | 100 | 100 |
Illumination cultivation stage nutrient solution has been heightened the content of saltpetre, ammonium nitrate, has increased iron fertilizer ironic citrate, promotes photosynthesis, makes Plant Leaf, footpath Fast Growth.Dark cultivate 15 days and illumination cultivation after 75 days Herba Anoectochili roxburghii center line average be 13.9cm, increase 8.5cm, average strain weight reaches 2.6g, weightening finish 1.5g, survival rate of plant 100%.
But, there is light to cultivate 90 days, illumination every day 9h, intensity is 1700-2000 Lux.After 90 days, Herba Anoectochili roxburghii center line average is 11.7cm, increases 5.3cm, and average strain weight reaches 2.3g, weightening finish 1.2g, survival rate of plant 100%.
Dark cultivation+illumination cultivation is heavily better than illumination cultivation in height of seedling, average strain, and two kinds of training methods the results are shown in Table 3 in 90 days.
The impact of table 3 varying environment control mode on Herba Anoectochili roxburghii
Nutrient solution prescription of the present invention mates completely for the nutritional needs of Anoectochilus roxburghii, and dietetic alimentation is more reasonable.Dark culture environment is conducive to taking root of Anoectochilus roxburghii and grows tall.Described NFT hydroponic device can carry out nutrient solution circulation, and the oxygen level that its nutritive medium is cultivated in liquid circulation is abundant, can allow all cultivation bodies fully and uniformly absorb nutrient, robust plant, and surviving rate obviously improves, and is highly suitable for industrialization and produces.
Claims (5)
1. a water planting implantation methods for Anoectochilus roxburghii, is characterized in that: get Anoectochilus roxburghii tissue-culture container seedling, clean up, move to hydroponic device and cultivate, the tissue cultured seedling of Herba Anoectochili roxburghii is placed in the cultivation bed on hydroponic device, and only root soaks in nutrient solution;
Water planting plantation is divided into two stages, dark cultivation 15 days of first stage, 27 ± 2 DEG C of room temps, humidity 80%-90%, nutrient composition is: saltpetre 113-148 mg/L, ammonium nitrate 62-85 mg/L, potassium primary phosphate 60-80 mg/L, magnesium sulfate heptahydrate 110-165 mg/L, four water-calcium nitrate 230-320 mg/L, potassiumiodide 0.05-0.08 mg/L, boric acid 2.2-3.0 mg/L, four water manganous sulfate 1.2-1.8 mg/L, Zinc Sulphate Heptahydrate 0.02-0.04 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.04 mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 12.0-16.4mg/L, iron vitriol 8.9-12.2mg/L, pH value 5.9,
Subordinate phase has light to cultivate 75 days, 27 ± 2 DEG C of room temps, humidity 80%-90%, illumination every day 9h, intensity is 1700-2000 Lux, nutrient composition is: saltpetre 122-183 mg/L, ammonium nitrate 82-98 mg/L, potassium primary phosphate 60-90 mg/L, magnesium sulfate heptahydrate 108-162 mg/L, four water-calcium nitrate 227-340 mg/L, potassiumiodide 0.06-0.08 mg/L, boric acid 2.3-3.5 mg/L, four water manganous sulfate 1.3-1.9 mg/L, Zinc Sulphate Heptahydrate 0.02-0.04 mg/L, Sodium orthomolybdate 0.02-0.04 mg/L, cupric sulfate pentahydrate 0.03-0.06mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 11.9-17.8mg/L, iron vitriol 8.8-13.3 mg/L, ironic citrate 14.3-21.5 mg/L, pH value 5.9.
2. the water planting implantation methods of a kind of Anoectochilus roxburghii as claimed in claim 1, it is characterized in that: the described first stage, the dark nutrient composition of cultivating was: saltpetre 123 mg/L, ammonium nitrate 71 mg/L, potassium primary phosphate 75 mg/L, magnesium sulfate heptahydrate 130 mg/L, four water-calcium nitrate 284 mg/L, potassiumiodide 0.07 mg/L, boric acid 2.9 mg/L, four water manganous sulfate 1.6 mg/L, Zinc Sulphate Heptahydrate 0.03 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.04 mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 13.4 mg/L, iron vitriol 10.0 mg/L, pH value 5.9.
3. the water planting implantation methods of a kind of Anoectochilus roxburghii as claimed in claim 1, it is characterized in that: described subordinate phase has light cultivation nutrient composition to be: saltpetre 153 mg/L, ammonium nitrate 82 mg/L, potassium primary phosphate 75 mg/L, magnesium sulfate heptahydrate 135 mg/L, four water-calcium nitrate 284 mg/L, potassiumiodide 0.07 mg/L, boric acid 2.9 mg/L, four water manganous sulfate 1.6 mg/L, Zinc Sulphate Heptahydrate 0.03 mg/L, Sodium orthomolybdate 0.03 mg/L, cupric sulfate pentahydrate 0.06mg/L, cobalt chloride 0.01 mg/L, disodium ethylene diamine tetraacetate 14.9 mg/L, iron vitriol 11.1 mg/L, ironic citrate 17.9 mg/L, pH value 5.9.
4. the water planting implantation methods of a kind of Anoectochilus roxburghii as claimed in claim 1, it is characterized in that: the dark cultivation of described first stage and subordinate phase have light to cultivate in the time of preparation molysite, after disodium ethylene diamine tetraacetate, two kinds of compounds of iron vitriol are dissolved respectively, remix is 80 DEG C of water-bath chelatings 30 minutes, then adds in nutrient solution.
5. the water planting implantation methods of a kind of Anoectochilus roxburghii as claimed in claim 1, is characterized in that: the described first stage is dark cultivates and subordinate phase has light to use phosphoric acid to determine pH value in cultivating.
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CN105165581A (en) * | 2015-09-23 | 2015-12-23 | 江西省科学院生物资源研究所 | Chili hydroponic method |
CN105830884A (en) * | 2016-03-29 | 2016-08-10 | 福建农林大学 | Ecological-simulated water-surface cultivation method for Anoectochilus roxburghii |
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CN110511091A (en) * | 2019-09-29 | 2019-11-29 | 西藏思思牧草科技有限公司 | Soilless culture nutrient fluid and the soilless culture method of herbage |
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