CN103460971A - Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings - Google Patents
Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings Download PDFInfo
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Abstract
The invention discloses a method for improving the transplanting survival rate of trichosanthes kirilowii tissue culture seedlings. According to the method, the antibacterial ability of the trichosanthes kirilowii tissue culture seedlings is enhanced by proportioning different substrate varieties with different ratios, seedling-hardening and domesticating processing, transplanting and managing in a restoring growing period after the transplanting. By the method disclosed by the invention, the trichosanthes kirilowii tissue culture seedlings are basically suitable for the external large-land growth environment, the plant root system is thick and strong, the new sprouts are good in growing vigor, the rattans are robust, and the transplanting survival rate can achieve 80-95% which is greatly improved compared with the survival rate of 10-20% of the traditional method. The method is convenient to operate, low in price and suitable for processing in batches and industrialized production, and has excellent economical efficiency and social benefits.
Description
Technical field
The invention belongs to the plant tissue culture field of growing seedlings, specifically relate to hardening, substrate preparation, transplanting and transplant after management improve the method for snakegourd group training transplantation of seedlings survival rate.
Background technology
Snakegourd [Trichosanthes kirilowii Maxim] is Curcurbitaceae snake gourd herbaceous perennial vine plant, and the another name Snakegourd Fruit, be a kind of dioecian plant, is China's tradition parts of generic medicinal plants.The snakegourd whole body is all precious, and its fruit, root all can be used as medicine, and seed claims semen trichosanthis, and root is root of Chinese trichosanthes, has clearing heat and eliminating phlegm, relieving chest stiffness to dissipate mass, moisturizes laxation, the effect such as detumescence and apocenosis.Edible Chinese juniper beach wormwood seed is nutritious, and hypertension, high fat of blood, high cholesterol, acute myocardial ischemia are had to significant protective effect, is middle-aged and old first-selected green health care foods.Simultaneously, Seeds of Trichosanthes kirilowii can improve body immune function, and weight reducing, beauty functions are arranged.Melon seeds outward appearance brown after frying is gorgeous, seed benevolence is full, the mouthfeel profit is continuous, crisp and fragrant is special, and enjoying endless aftertastes of food, be described as " king of melon seeds ".
In recent years, the research and comparison of snakegourd tissue culture technology was many, mainly contained the research of snakegourd quick propagating technology; The snakegourd tissue is cultivated and the research of non-test-tube plantlet fast breeding technique; The research of plant regeneration in the cultivation of snakegourd tissue; The detoxification cultivation of snakegourd shoot tip meristem and Plantlet Differentiation etc.The snakegourd tissue culture technology has tended to ripe at present, but the artificial large-area China that is planted in of snakegourd is also in the starting stage, development speed is relatively slow, main cause is that snakegourd group training transplantation of seedlings survival rate is low, especially 1-2 of the firm bottle outlet of group training seedling month, be mainly because group training seedling is large in the growing environment difference of the environment of culturing room and transplanting, had a strong impact on the transplanting survival rate of snakegourd group training seedling, fail to make group culturation rapid propagating technology to be applied to production practices.
At present, almost do not have R&D institution and enterprise to carry out correlative study for hardening, domestication, the transplanting medium of snakegourd group training seedling.By hardening, domestication, the transplanting medium research of carrying out snakegourd group training seedling, this technology can improve the transplanting survival rate of snakegourd group training seedling greatly.
Summary of the invention
The objective of the invention is to be to provide a kind of method that improves snakegourd group training transplantation of seedlings success rate, can strengthen the antibacterial ability of snakegourd group training seedling, make it better adapt to external environment, can improve the survival rate of snakegourd group training seedling.
For achieving the above object, the present invention takes following technical measures:
The technical solution adopted in the present invention is by substrate preparation and processing, hardening, transplanting and recovery growing season management, makes the snakegourd plant little by little adapt to extraneous natural environment,
A kind of method that improves snakegourd group training transplantation of seedlings survival rate, its step is as follows
(1) tissue is cultivated:
In the indoor tissue cultivation of carrying out the snakegourd seedling according to conventional method, in the present invention, the snakegourd prescription of rooting medium is MS+0.2 (mg/L) NAA.
(2) in-bottle seeding:
Annual late March to late June is chosen snakegourd height of seedling 3-5cm, leaf 2-3 sheet, the aseptic bottle seedling of root 3-5 bar is put in the green house under natural environment, band bottle hardening 5-7d, then open and cultivate the bottle cap hardening, add appropriate distilled water submergence snakegourd root in blake bottle, keep every day air humidity to be greater than 70%, place 3-5d after uncork.
(3) the outer hardening of bottle:
Substrate preparation and processing: the mixture of river sand, vermiculite, peat of take is hardening matrix, and described hardening matrix is by bactericide disinfection, then sabot.
Described river sand is the 4-10 order, apparent density 2-3kg/m
3, bulk density 1-2kg/m
3, modulus of fineness is less than 3, and clay content is less than 2%, and its granularity is between 2.5mm-5mm.
Described vermiculite is the 4-10 order, and specification is 2-4mm, and density is 2.4-2.7g/cm
3.
Described peat proportion is 1.20-1.60, and water content is 70-85%, and the content of organic matter is more than 30%, and content of humic acid is more than 30%, and the pH value is 5-6.5.
In described matrix, the volume of mixture of river sand, vermiculite and peat is than being 1-2:1-2:1-2.
Described matrix bactericide is carbendazim, and the carbendazim that every 1 cubic metre of matrix is sprayed 10L800 times of liquid carries out disinfection.
The vinyl disc that described dress seedling is used is 72 hole seedling culture hole plates, cave, circle 72 holes (6 * 9) dish: upper bore: 40mm, and lower bore: 21mm, highly: 45mm, volume: 35ml, cave dish size: 532mm * 278mm.
Transplant: after uncork is placed, with tweezers, the plant by tissue culture bottle is gently pressed from both sides out, with running water, the medium on plant is rinsed well, and then hung 1h(and hang environment: 15-25 ℃, air humidity remains more than 75%), move in the plastics cave dish be equipped with through sterilization matrix, the strain of planting of each hole, cave, root system stretches as far as possible, compresses matrix while planting as far as possible, the mode of spraying with watering pot after group training seedling is transplanted is watered permeable, keeps air humidity at 70-80%; After the snakegourd seedling grows young leaves and Xin Gen, front 5-7d humidity is controlled at 70-80%, reduces gradually humidity later, and the scope that the humidity of average every day descends is 1%-5%, until identical with the outer land for growing field crops of canopy ambient humidity.
The method of described controlled humidity, the conventional method that available cultivation time control is wet or matrix water for the first time permeable after, directly put into the shed of the transparent plastic film covering of wide 1.2m, long 10m, high 0.5m, the shed surrounding covers and the compacting film with soil, every morning is rod tapped shed for 8:00-9:00, allow the globule formed in the shed plastic film drop onto in seedling-cultivating tray, utilize water cycle to keep in canopy humidity can using water wisely.
(4) recover growing season management: after the snakegourd seedling grows young leaves and Xin Gen, every 5-7d adds and uses the urea that mass volume ratio is 0.05%-0.1%, and plant grows to the 10cm left and right, when root system and matrix can be agglomerating be deviate from, gets final product placing and carry out Field planting.
Suitable shading during whole hardening, make the 35%-40% that intensity of illumination is natural daylight, and intensity of illumination is controlled at 0.6-1.0 ten thousand lux.
During the outer hardening of bottle and recovery growing season management, soil humidity remains on 40-60%.
Compared with prior art, the invention has the advantages that:
A kind of method that improves snakegourd group training transplantation of seedlings survival rate is disclosed first, the method carry out different proportion proportioning, hardening acclimation, transplanting by the different substrates kind and transplant after the recovery growing season management strengthen the antibacterial ability of snakegourd group training aseptic seedling, make it better adapt to external environment, can improve the survival rate of snakegourd group training seedling.The method is easy to operate, and expense is low, is suitable for batch process and batch production production, has good business efficiency and social benefit.
Hardening off method of the present invention has been done very large improvement, make antibacterial ability and the adaptive faculty of seedling that very large improvement arranged, the group training seedling of the substrate culture of taming by hardening adapts to extraneous grown in field environment substantially, plant root is sturdy, the young sprout growing way is good, rattan is healthy and strong, and its transplanting survival rate can reach 80%-95%.Compare and be greatly improved with the 10%-20% survival rate of conventional method.
The accompanying drawing explanation
Fig. 1 is the snakegourd effect photo of a kind of snakegourd group training method for transplanting of the present invention after 3 months;
Matrix in figure is matrix of the present invention.
Fig. 2 is the snakegourd effect photo of a kind of conventional snakegourd group training method for transplanting after 3 months;
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
A kind of method that improves snakegourd group training transplantation of seedlings survival rate:
(1) tissue is cultivated:
At the indoor tissue that carries out the snakegourd seedling according to conventional method, cultivate (list of references: Yang Lina, Liu Jie, Tao Jianmin. the tissue of snakegourd is cultivated and Fast-propagation [J]. the Jiangsu agricultural science, 2008,15 (4): 89-90.),
In the present embodiment, the snakegourd prescription of rooting medium is MS+0.2(mg/L) NAA.
(2) in-bottle seeding: on March 20th, 2013, by the high 3-5cm of 50 young plant, leaf 2-3 sheet, the snakegourd aseptic seedling of root 3-5 bar is put into hardening 7d in green house, then open and cultivate the bottle cap hardening, add appropriate distilled water submergence snakegourd root in blake bottle, keep air humidity to be greater than 70%, place 3d.
(3) the outer hardening of bottle
Substrate preparation and processing: take river sand, vermiculite, peat volume ratio as 1:1:2 carries out proportioning, in the plastics cave dish of packing into, carry out matrix with carbendazim and disinfect, the carbendazim that every 1 cubic metre of matrix is sprayed 10L800 times of liquid carries out disinfection.
Described river sand is the 4-10 order, apparent density 2-3kg/m
3, bulk density 1-2kg/m
3, modulus of fineness is less than 3, and clay content is less than 2%, and its granularity is between 2.5mm-5mm.
Described vermiculite is the 4-10 order, and specification is 2-4mm, and density is 2.4-2.7g/cm
3.
Described peat proportion is 1.20-1.60, and water content is 70-85%, and the content of organic matter is more than 30%, and content of humic acid is more than 30%, and the pH value is 5-6.5.
Transplant: on March 30th, 2013, transplanted, during transplanting with tweezers the plant by tissue culture bottle gently press from both sides out, with running water, the medium on plant is rinsed well, and then hang 1h and transplanted (hang environment: temperature 15-25 ℃, air humidity remains more than 75%), move in the plastics cave dish be equipped with through sterilization matrix, the strain of planting of each hole, cave, root system stretches as far as possible, compresses matrix as far as possible, and the mode of spraying with watering pot after transplanting is watered permeable.Then put into the shed of the transparent plastic film covering of wide 1.2m, long 10m, high 0.5m, the shed surrounding covers and the compacting film with soil, every morning is rod tapped shed for 8:00-9:00, allow the interior globule formed of shed plastic film drop onto in seedling-cultivating tray, utilize water cycle to keep in canopy humidity can using water wisely, keep air humidity at 70-80%.After 15d, plant grows young leaves and Xin Gen, then first open the plastic film at shed two, make air humidity in shed be controlled at 70-80%, after 5d, again plastic film is opened fully, reduce gradually humidity every day, the scope that the humidity of average every day can descend is 1%-5%, until identical with the outer land for growing field crops of canopy ambient humidity.
(4) recover growing season management: after 15 days, the snakegourd seedling grows young leaves and Xin Gen, then every 6d adds and uses the urea that mass volume ratio is 0.05%-0.1% the snakegourd seedling, and after 30d, plant grows to the 10cm left and right, when root system and matrix can be agglomerating be deviate from, placing carries out Field planting.
Suitable shading during whole hardening, make the 35%-40% that intensity of illumination is natural daylight, and intensity of illumination is controlled at 0.6-1.0 ten thousand lux.
The black sunshade net of shading rate 50% for shading.
During the outer hardening of bottle and recovery growing season management, soil humidity remains on 40-60%.
Choose 50 strains and carry out field planting in having refined the plant of seedling, totally 42 strains survive, and survival rate is 84%.
Embodiment 2:
Conventional snakegourd group training method for transplanting:
With embodiment 1, its difference is: will organize the training seedling and be placed on natural conditions lower refining seedling 5-7d, and then open and cultivate bottle cap again after hardening 3-5d, and directly be transplanted to large Tanaka and go.By in above-mentioned plant of having refined seedling, choosing 50 strains, planted, totally 8 strains survive, survival rate only 16%.
Above-described embodiment does not limit the present invention in any form, and the present invention also is not limited to above-mentioned giving an example.The use of the art is equal to replaces or technical scheme that the mode of equivalent transformation obtains, all drops in protection scope of the present invention.
Claims (2)
1. one kind is improved the method that the snakegourd group is trained the transplantation of seedlings survival rate, and its step is as follows:
(1) tissue is cultivated:
In the indoor tissue cultivation of carrying out the snakegourd seedling according to conventional method, the snakegourd prescription of rooting medium is MS+0.2 mg/L NAA;
(2) in-bottle seeding:
Annual late March to late June is chosen height of seedling 3-5cm, leaf 2-3 sheet, the aseptic bottle snakegourd seedling of root 3-5 bar is put in the green house under natural environment, band bottle hardening 5-7d, then open and cultivate the bottle cap hardening, add appropriate distilled water submergence snakegourd root in blake bottle, keep every day air humidity to be greater than 70%, place 3-5d after uncork;
(3) the outer hardening of bottle:
Substrate preparation and processing: the mixture of river sand, vermiculite, peat of take is hardening matrix, and described hardening matrix is by bactericide disinfection, then sabot;
Described river sand is the 4-10 order, apparent density 2-3kg/m
3, bulk density 1-2kg/m
3, modulus of fineness is less than 3, and clay content is less than 2%, and its granularity is between 2.5mm-5mm;
Described vermiculite is the 4-10 order, and specification is 2-4mm, and density is 2.4-2.7g/cm
3;
Described peat proportion is 1.20-1.60, and water content is 70-85%, and the content of organic matter is more than 30%, and content of humic acid is more than 30%, and the pH value is 5-6.5;
In described matrix, the volume of mixture of river sand, vermiculite and peat is than being 1-2:1-2:1-2;
Described matrix bactericide is carbendazim, and the carbendazim that every 1 cubic metre of matrix is sprayed 800 times of liquid of 10L carries out disinfection;
The vinyl disc that described dress seedling is used is 72 hole seedling culture hole plates, cave, circle 72 holes (6 * 9) dish: upper bore: 40mm, and lower bore: 21 mm, highly: 45 mm, volume: 35ml, cave dish size: 532mm * 278mm;
Transplant: uncork is pressed from both sides out the plant in tissue culture bottle with tweezers after placing, and with running water, the medium on plant is rinsed well, and then is hung 1h, hangs environment: 15-25 ℃, and air humidity remains more than 75%; Move in the plastics cave dish be equipped with through sterilization matrix, the strain of planting of each hole, cave, root system stretches, and compresses matrix while planting, and the mode of spraying with watering pot after group training seedling is transplanted is watered permeable, keeps air humidity at 70-80%; After the snakegourd seedling grows young leaves and Xin Gen, front 5-7d air humidity is controlled at 70%-80%, reduces gradually humidity later, and the scope that the humidity of average every day descends is 1%-5%, until identical with the outer land for growing field crops of canopy ambient humidity;
(4) recover growing season management: after the snakegourd seedling grows young leaves and Xin Gen, every 5-7d adds and uses the urea that mass volume ratio is 0.05%-0.1%, and plant grows to the 10cm left and right, when root system and matrix can be agglomerating be deviate from, gets final product placing and carry out Field planting;
Suitable shading during whole hardening, make the 35%-40% that intensity of illumination is natural daylight, and intensity of illumination is controlled at 0.6-1.0 ten thousand lux;
During the outer hardening of bottle and recovery growing season management, soil humidity remains on 40-60%.
2. raising snakegourd group according to claim 1 is trained the method for transplantation of seedlings survival rate, while transplanting rear controlled humidity, matrix water for the first time permeable after, directly put into the shed of the transparent plastic film covering of wide 1.2m, long 10m, high 0.5m, the shed surrounding covers and the compacting film with soil, every morning is rod tapped shed for 8:00-9:00, allows in the shed plastic film globule formed drop onto in seedling-cultivating tray, utilizes water cycle to keep in canopy humidity can using water wisely.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145700A (en) * | 2014-08-28 | 2014-11-19 | 广州白云山中一药业有限公司 | Method for planting radix trichosanthis medicine |
| CN104521756A (en) * | 2014-12-24 | 2015-04-22 | 广西大学 | Method for producing trichosanthes tissue culture seedlings |
| CN106417033A (en) * | 2016-11-10 | 2017-02-22 | 聊城大学 | Rapid Gaotang trichosanthes kirilowii propagation method |
| CN108077022A (en) * | 2017-12-18 | 2018-05-29 | 长沙智博生物科技有限公司 | For the matrix of blueberry hardening and blueberry hardening off method |
| CN111226770A (en) * | 2020-03-07 | 2020-06-05 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Liquid seedling hardening method for improving transplanting survival rate of tissue culture seedlings of cinnamomum kanehirae |
| CN111345230A (en) * | 2020-04-29 | 2020-06-30 | 蒙树生态建设集团有限公司 | Seedling hardening method for iris tissue culture seedlings |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278642A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for training tissue culture seedling of tomato |
| CN101496497A (en) * | 2008-08-09 | 2009-08-05 | 周小红 | Seedling exercising technique for test-tube plantlet with full sunshine and spray |
| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
-
2013
- 2013-09-30 CN CN201310459162.7A patent/CN103460971B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278642A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for training tissue culture seedling of tomato |
| CN101496497A (en) * | 2008-08-09 | 2009-08-05 | 周小红 | Seedling exercising technique for test-tube plantlet with full sunshine and spray |
| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
Non-Patent Citations (5)
| Title |
|---|
| 朱勤等: "食籽栝楼离体快繁的初步研究", 《安徽农业科学》, no. 16, 10 June 2006 (2006-06-10), pages 3965 - 3966 * |
| 邓朝军等: "园艺植物组织培养苗的移栽技术", 《福建果树》, no. 2, 30 June 2006 (2006-06-30), pages 15 - 17 * |
| 郦平等: "食用吊瓜工厂化育苗技术规程", 《上海蔬菜》, no. 5, 15 October 2009 (2009-10-15), pages 50 - 53 * |
| 陈丽英等: "温湿度调控是核心——谈组培苗的炼苗技术", 《中国花卉园艺》, no. 7, 1 April 2004 (2004-04-01), pages 32 - 33 * |
| 陈胜等: "栝楼试管苗瓶外生根技术研究", 《北方园艺》, no. 12, 30 June 2011 (2011-06-30), pages 113 - 115 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145700A (en) * | 2014-08-28 | 2014-11-19 | 广州白云山中一药业有限公司 | Method for planting radix trichosanthis medicine |
| CN104521756A (en) * | 2014-12-24 | 2015-04-22 | 广西大学 | Method for producing trichosanthes tissue culture seedlings |
| CN106417033A (en) * | 2016-11-10 | 2017-02-22 | 聊城大学 | Rapid Gaotang trichosanthes kirilowii propagation method |
| CN108077022A (en) * | 2017-12-18 | 2018-05-29 | 长沙智博生物科技有限公司 | For the matrix of blueberry hardening and blueberry hardening off method |
| CN111226770A (en) * | 2020-03-07 | 2020-06-05 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Liquid seedling hardening method for improving transplanting survival rate of tissue culture seedlings of cinnamomum kanehirae |
| CN111345230A (en) * | 2020-04-29 | 2020-06-30 | 蒙树生态建设集团有限公司 | Seedling hardening method for iris tissue culture seedlings |
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