CN103392460A - Method for propagating wild Vietnamese sophora root through cuttage - Google Patents
Method for propagating wild Vietnamese sophora root through cuttage Download PDFInfo
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- CN103392460A CN103392460A CN2013102418417A CN201310241841A CN103392460A CN 103392460 A CN103392460 A CN 103392460A CN 2013102418417 A CN2013102418417 A CN 2013102418417A CN 201310241841 A CN201310241841 A CN 201310241841A CN 103392460 A CN103392460 A CN 103392460A
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Abstract
The invention relates to a method for propagating a wild Vietnamese sophora root through cottage. The method comprises the following steps that (1) a wild Vietnamese sophora rootstalk is cut, (2) rootstalk sections are placed into nutrient bags, (3) heat-preservation cultivation is conducted for 19-21 days, and (4) seedlings are transplanted to a field. The method solves the problems that in traditional tissue culture, the requirements for indoor propagating conditions are high, and pollution is prone to occurring during transplanting and provides a good basis for vegetative propagation of Vietnamese sophora root plants. Moreover, large-scale practicability realization and industrialization are easy, and a technical path which realizes high survival rate, low cost and strong practicability is provided for large scale tissue culture propagation, production and development in the future biotechnological field.
Description
Technical field
The present invention relates to technical field of plant culture, relates in particular to a kind of cuttage expanding propagation method of wild subprostrate sophora.
Background technology
Subprostrate sophora (Vietnamese Sophora Root) is Dicotyledoneae pulse family Euchrest plant, has another name called sophora tonkinensis Gapnep, root of subprostrate sophora, lima bean lotus, mainly is distributed in Guangxi, Guizhou, Yunnan.Subprostrate sophora is the shrub of high 1～2m, be born in the Limestone Mountain or rock seam of subtropics or temperate zone height above sea level 1000～2000m, be scattered about like the stars in the evergreen broad-leaved sylvan life of cheuch small stream limit, like environment dark and damp, that humus is abundant, usually grow in red soil or yellow earth, the pH value is 4.5～5.5.Therefore, the artificial cultivation of wild subprostrate sophora is difficult for realizing.The dry root and rhizome of subprostrate sophora is common Chinese medicine, but Qinghuo Jiedu, swelling and pain relieving is usually used in throat swelling and aching of gum, cough with lung heat's polydipsia, jaundice, constipation with heat retention.The effects such as that sophorae subprostratae radix extract also has is antitumor, resisting pathogenic microbes, have higher medical value, causes people to the excessive collection of subprostrate sophora, and wild resource is sharply descended.In " the national key protected wild plants register " that promulgate 1997 and 1999; lima bean lotus (being subprostrate sophora) is included as second class protection kind (" Chinese Plants Red Data Book " (first) enters the three-level protective kind by its example), and its stock number in the whole nation is also comparatively rare as seen.Therefore, the cultivation subprostrate sophora, especially wild subprostrate sophora, have great economic worth and important meaning.
The modes of reproduction of subprostrate sophora is generally seminal propagation, but, because its germinating capacity is low, grows slower, and fertility is low, thus the normal method for tissue culture that adopts in prior art, to reach the purpose of Fast-propagation subprostrate sophora.But the germination rate of the seed of wild subprostrate sophora is very low, and higher to the germination environmental requirement, makes the conversion ratio of seed-seedling also very low, adds its poor growth, has seriously restricted the expansion of population.Especially after people cause the destruction in subprostrate sophora habitat to the destruction of forest, though dropping to, the fertility of subprostrate sophora and viability can grow reluctantly, hardly reproducible stage.So general subprostrate sophora tissue culture propagation method not necessarily is applicable to the cultivation of wild subprostrate sophora.
quick breeding method for tissue culture as the disclosed a kind of root of subprostrate sophora of CN102301951B, to get the root of subprostrate sophora seed to carry out disinfection as explant, explant after sterilization is placed in to the MS minimal medium induces germination to obtain in vitro cuttings, described in vitro cuttings is placed in to the MS propagating culture medium and carries out plantlet bud propagation cultivation acquisition Multiple Buds, described Multiple Buds is placed in to MS strong seedling culture base to carry out strong seedling culture and obtains healthy and strong plant, described healthy and strong plant is placed in to the cultivation of MS root media and obtains complete band offspring, taking whole band offspring carries out transplanting in husky bed growth one month after hardening, transplant to land for growing field crops again.This scheme is to germinate and obtain in vitro cuttings take the root of subprostrate sophora seed as explant induction, and its cuttage that is not suitable for wild root of subprostrate sophora is expanded numerous.
And for example the disclosed a kind of root of subprostrate sophora group of CN102715090B is cultivated seedling industrialized production method, to obtain Multiple Buds by after the root of subprostrate sophora seed disinfection, being inoculated on propagating culture medium to cultivate, Multiple Buds is cut into to stem with bud, be laid on the integrated solid medium of breeding rejuvenation face, with tweezers, 1/2 of stem section is pressed in medium again, inoculate after complete to change in culturing room and cultivated 15 days, and then change under the scattering natural lighting and to cultivate 15 days, obtain mounted blade, healthy and strong branch that degree of lignification is higher; Again healthy and strong branch is cut, be inoculated in the hardening synchronization medium of taking root, indoor cultivation 7 days, new root changes the glass booth over to after starting to sprout, the hardening of taking root synchronization was cultivated 25 days, obtain well developed root system, the whole plant of suitable transplanting, the seedling of finally taking root was transplanted to the seedling bed of smooth intensive cultivation at dusk in the cloudy day.This scheme is that after the Multiple Buds that the root of subprostrate sophora seed culture obtains was cut into to stem with bud, the continuation cultivation obtained healthy and strong branch, and its cuttage that is not suitable for wild root of subprostrate sophora is expanded numerous.
The rooting method of a CN102499083B subprostrate sophora root tissue culture seedling leaf stalks for another example, to strong sprout in MS strong seedling culture base by the root of subprostrate sophora tissue culture plant inoculation, make to organize the training seedling leaf and launch to obtain the complete higher band handle compound leaf of degree of lignification, to be with the handle compound leaf to be placed in the MS root media and cultivate, root induction.This scheme is after strong sprout in medium, to obtain being with the handle compound leaf by the root of subprostrate sophora tissue culture plant inoculation, and its cuttage that also is not suitable for wild root of subprostrate sophora is expanded numerous.
Above prior art is to adopt root of subprostrate sophora (being subprostrate sophora) seed to germinate and obtain after test-tube plantlet Fast-propagation and cultivate and obtain Multiple Buds as explant induction, carry out again strong seedling culture and obtain healthy and strong plant, continue to cultivate and obtain complete band offspring, after hardening, be transplanted in husky bed, finally transplant to land for growing field crops; Or by the subprostrate sophora tissue culture plant inoculation to root induction then in strong sprout in medium.Above subprostrate sophora tissue is cultivated strict especially to the requirement of the various growth conditionss such as temperature, humidity, illumination and gnotobasis.Particularly move past in journey outside, if a certain link operate miss causes mould (carefully) fungi pollution, consequence is hardly imaginable, when serious, can cause whole expansion numerous and develop unsuccessfully.Can the test-tube plantlet of in-vitro propagate be widely used in Development and Production, and particularly can the wild plant kind benefit that obtain, depend on whether test-tube plantlet has higher survival rate.The survival rate of wild subprostrate sophora is low, and the test-tube seedling transplanting cost is higher, moves into motility rate outward low, and the tissue culture expanding propagation cost is high, is difficult to practical, batch production on a large scale.
Summary of the invention
In order to solve the problems of the technologies described above, the object of the present invention is to provide a kind of cuttage expanding propagation method of wild subprostrate sophora.
The invention provides following technical scheme:
A kind of cuttage expanding propagation method of wild subprostrate sophora is characterized in that: comprise the following steps:
(1) cutting of wild subprostrate sophora stem:
The stem of wild subprostrate sophora is cut to the stem with bud of 10～12cm, every section 3～4 buds;
(2) the stem section is packed into nutritious bag:
2/3 of cutting gained stem section in step (1) is implanted in nutritious bag, and 1/3 is exposed outside nutritious bag, on the stem section of described implantation nutritious bag, 2～3 buds arranged, and the pH value is housed is 6～7 the humus soil that is comprised of the dirty oil sandy soil in described nutritious bag;
(3) heat insulating culture is 19～21 days:
It is 15 ℃～20 ℃ that the nutritious bag that is implanted with the stem section in step (2) is placed in to temperature, and humidity is, in 50%～70% environment, to water once in every 2 days, cultivates 19～21 days, obtains the subprostrate sophora seedling of taking root;
(4) move into land for growing field crops:
By cultivating the complete subprostrate sophora seedling of taking root in step (3), together with the nutritious bag cuttage, enter large Tanaka, the spacing in the rows of cuttage is 40cm, and the row spacing of field is 50cm.
Humus soil in described step (2) in nutritious bag is the dirty oil sandy soil that the pH value is 6～7 to be put into to the dried pot of high temperature parch with 70～80 ℃ make.
Beneficial effect of the present invention: solved the numerous conditional request of the indoor expansion of traditional group training high, change the problem of pollution outward, for vegetative propagation lima bean lotus plant provides good foundation, be easy to practical, batch production on a large scale, for the extensive tissue culture expanding propagation of biological technical field, production development from now on provide, realize high-servival rate, low cost, practical strong technological approaches.
In prior art, usually adopting the subprostrate sophora seed is that material is organized cultivation, and the inventive method is to adopt the stem section of wild subprostrate sophora to carry out cuttage to expand numerous, adopted the nutritious bag that frying dirty oil sandy soil are housed, innovated the propagation method of wild subprostrate sophora, and method is simple to operate, and production cost is lower.It is 15 ℃～20 ℃ that the nutritious bag that is implanted with wild subprostrate sophora stem section is placed in to temperature, and humidity is in 50%～70% environment, has solved the high problem of the traditional group training numerous conditional request of indoor expansion.When the complete subprostrate sophora of transplanting cultivation is taken root seedling, together with the nutritious bag cuttage, enter large Tanaka, solved the outer problem of changing pollution.
Because subprostrate sophora self has nitrogen fixation, its seed need to be dressed seed with rhizobium prior to seeding usually, to prevent root rot.The humus soil that adopts in nutritious bag of the present invention is the dirty oil sandy soil that the pH value is 6～7 to be put into to the dried pot of high temperature parch with 70～80 ℃ make, can effectively kill the miscellaneous bacteria in humus soil, can imitate wild soil environment again, avoid the subprostrate sophora can't smooth growth because of miscellaneous bacteria in rooting process.The dirty oil sandy soil are a kind of sandy black waxy soils, belong to the black loess penus of chernozem subclass, and this soil species matrix is the Loessial sediments, and color is dark brown or burgundy is granular and crumbled structure, and the content of organic matter is on average greater than 5.0%.Subprostrate sophora grows in red soil and yellow earth usually, but, by the humus soil middle fertility that the dirty oil sandy soil are made, is neutral and has the characteristics such as Li Shui, moisturizing, fertilizer conservation to subacidity, is that the ideal of cultivation stem section is planted material, is also the splendid soil of cultivation subprostrate sophora.Adopting the dirty oil sandy soil of middle fertility, is that because himself has nitrogen fixing capacity, the nitrogenous fertilizer that therefore need to use is very not many because lima bean lotus (being subprostrate sophora) is though need nitrogen many; Low middling fertility field, the timely and appropriate discovery applied nitrogen has effect of increasing production preferably, and the field that fertility is higher is not obvious, uses too much not only waste, but also can cause the underproduction.
The specific embodiment mode
Embodiment 1
(1) cutting of wild subprostrate sophora stem:
The stem of wild subprostrate sophora is cut to the stem with bud of 11cm, every section 4 buds; Stem with bud is placed on superclean bench, puts into 0.1% the mercuric chloride solution 4min that carries out disinfection, after taking-up, use again 70% alcohol rinse once, with aseptic water washing 4 times;
(2) the stem section is packed into nutritious bag:
2/3 of cutting gained stem section in step (1) is implanted in nutritious bag, and 1/3 is exposed outside nutritious bag, on the stem section of described implantation nutritious bag, 3 buds arranged, and the pH value is housed is 6.5 humus soil in described nutritious bag; Humus soil in described nutritious bag is the dirty oil sandy soil that the pH value is 6.5 to be put into to the dried pot of high temperature parch with 75 ℃ make;
(3) heat insulating culture is 20 days:
It is 18 ℃ that the nutritious bag that is implanted with the stem section in step (2) is placed in to temperature, and humidity is, in 60% environment, to water once in every 2 days, cultivates 20 days, obtains the subprostrate sophora seedling of taking root; In cultivation process, prevent a bag internal contamination; In the aforementioned temperature scope, strengthen gradually intensity of illumination in greenhouse, make subprostrate sophora seedling in nutritious bag illumination, the temperature and humidity condition in conforming gradually, when intensity of illumination is high, carry out the shading management, prevent from burning seedling;
(4) move into land for growing field crops:
By cultivating the complete subprostrate sophora seedling of taking root in step (3), together with the nutritious bag cuttage, enter large Tanaka, the spacing in the rows of cuttage is 40cm, and the row spacing of field is 50cm.Before cuttage, land for growing field crops is watered permeable, guarantee cuttage survival rate.After the cuttage of subprostrate sophora seedling enters in land for growing field crops, spray carbendazim or other sterilized pharmaceutics, prevent that seedling from catching an illness.
The cuttage of adopting said method to carry out wild subprostrate sophora is expanded numerous, simple to operate, and efficiency is higher than the traditional group training of subprostrate sophora, and difficult the pollution, and seedling stalwartness, growth indexes all are better than test tube transplants seedlings outward, moves into motility rate outward and can save seedling from damage.
Embodiment 2
(1) cutting of wild subprostrate sophora stem:
The stem of wild subprostrate sophora is cut to the stem with bud of 10cm, every section 3 buds;
(2) the stem section is packed into nutritious bag:
2/3 of cutting gained stem section in step (1) is implanted in nutritious bag, and 1/3 is exposed outside nutritious bag, on the stem section of described implantation nutritious bag, 2 buds arranged, and the pH value is housed is 6 humus soil in described nutritious bag;
Humus soil in described step (2) in nutritious bag is the dirty oil sandy soil that the pH value is 6 to be put into to the dried pot of high temperature parch with 70 ℃ make;
(3) heat insulating culture is 19 days:
It is 15 ℃ that the nutritious bag that is implanted with the stem section in step (2) is placed in to temperature, and humidity is, in 50% environment, to water once in every 2 days, cultivates 19 days, obtains the subprostrate sophora seedling of taking root;
(4) move into land for growing field crops:
By cultivating the complete subprostrate sophora seedling of taking root in step (3), together with the nutritious bag cuttage, enter large Tanaka, the spacing in the rows of cuttage is 40cm, and the row spacing of field is 50cm.
Embodiment 3
(1) cutting of wild subprostrate sophora stem:
The stem of wild subprostrate sophora is cut to the stem with bud of 12cm, every section 4 buds;
(2) the stem section is packed into nutritious bag:
2/3 of cutting gained stem section in step (1) is implanted in nutritious bag, and 1/3 is exposed outside nutritious bag, on the stem section of described implantation nutritious bag, 3 buds arranged, and the pH value is housed is 7 humus soil in described nutritious bag;
Humus soil in described step (2) in nutritious bag is the dirty oil sandy soil that the pH value is 7 to be put into to the dried pot of high temperature parch with 80 ℃ make;
(3) heat insulating culture is 21 days:
It is 20 ℃ that the nutritious bag that is implanted with the stem section in step (2) is placed in to temperature, and humidity is, in 70% environment, to water once in every 2 days, cultivates 21 days, obtains the subprostrate sophora seedling of taking root;
(4) move into land for growing field crops:
By cultivating the complete subprostrate sophora seedling of taking root in step (3), together with the nutritious bag cuttage, enter large Tanaka, the spacing in the rows of cuttage is 40cm, and the row spacing of field is 50cm.
Embodiment 4
(1) cutting of wild subprostrate sophora stem:
The stem of wild subprostrate sophora is cut to the stem with bud of 11m, every section 3 buds;
(2) the stem section is packed into nutritious bag:
2/3 of cutting gained stem section in step (1) is implanted in nutritious bag, and 1/3 is exposed outside nutritious bag, on the stem section of described implantation nutritious bag, 2 buds arranged, and the pH value is housed is 7 humus soil in described nutritious bag;
(3) heat insulating culture is 21 days:
It is 17 ℃ that the nutritious bag that is implanted with the stem section in step (2) is placed in to temperature, and humidity is, in 65% environment, to water once in every 2 days, cultivates 21 days, obtains the subprostrate sophora seedling of taking root;
(4) move into land for growing field crops:
By cultivating the complete subprostrate sophora seedling of taking root in step (3), together with the nutritious bag cuttage, enter large Tanaka, the spacing in the rows of cuttage is 40cm, and the row spacing of field is 50cm.
Get 10, wild subprostrate sophora seed, numbering group 1, adopt the disclosed a kind of root of subprostrate sophora group of CN102715090B to cultivate seedling industrialized production method, every subprostrate sophora seed organizing in 1 is organized respectively to cultivation, add up rooting rate and the survival rate of final subprostrate sophora seedling.Get again 20 sections of wild subprostrate sophora stem with bud, be equally divided into 2 groups, be numbered respectively group 2, group 3, adopt that the every section subprostrate sophora stem section of the method for the invention to organizing 2, in group 3 cultivated respectively, cuttage is expanded numerous, wherein, in the nutritious bag of group 2, adopt the common humus soil made from field, field soil, hay, fallen leaves, stalk, animal waste, bone bits, rice bran, brown sugar, flour, yeast, and organize the humus soil that nutritious bag in 3 adopts frying dirty oil sandy soil of the present invention, add up final subprostrate sophora seedling rooting rate and survival rate.The survival rate comparing result of wild subprostrate sophora is as shown in table 1.
The rooting rate of the wild subprostrate sophora of table 1 and survival rate
| Group | The material number | The number of taking root | The transplant survival number | Rooting rate (%) | Survival rate (%) |
| Group 1 | 10 | 7 | 6 | 70 | 60 |
| Group 2 | 10 | 6 | 5 | 60 | 50 |
| Group 3 | 10 | 9 | 8 | 90 | 80 |
As can be seen from Table 1, the rooting rate of group 3 and survival rate are higher than group 1 and group 2, namely adopt the method for the invention to cultivate wild subprostrate sophora plant to wild subprostrate sophora stem section, its rooting rate and survival rate are higher than the rooting rate and the survival rate that adopt prior art (organizing cultivation with the subprostrate sophora seed), and also culture matrix in nutritious bag adopts common technical scheme humous higher than adopting close with the technical program.Visible, technical solution of the present invention is applicable to the cuttage of wild subprostrate sophora and expands numerous.
Claims (2)
1. the cuttage expanding propagation method of a wild subprostrate sophora is characterized in that: comprise the following steps:
(1) cutting of wild subprostrate sophora stem:
The stem of wild subprostrate sophora is cut to the stem with bud of 10～12cm, every section 3～4 buds;
(2) the stem section is packed into nutritious bag:
2/3 of cutting gained stem section in step (1) is implanted in nutritious bag, and 1/3 is exposed outside nutritious bag, on the stem section of described implantation nutritious bag, 2～3 buds arranged, and the pH value is housed is 6～7 the humus soil that is comprised of the dirty oil sandy soil in described nutritious bag;
(3) heat insulating culture is 19～21 days:
It is 15 ℃～20 ℃ that the nutritious bag that is implanted with the stem section in step (2) is placed in to temperature, and humidity is, in 50%～70% environment, to water once in every 2 days, cultivates 19～21 days, obtains the subprostrate sophora seedling of taking root;
(4) move into land for growing field crops:
By cultivating the complete subprostrate sophora seedling of taking root in step (3), together with the nutritious bag cuttage, enter large Tanaka, the spacing in the rows of cuttage is 40cm, and the row spacing of field is 50cm.
2. preparation method according to claim 1 is characterized in that: the humus soil in described step (2) in nutritious bag is the dirty oil sandy soil that pH value is 6～7 to be put into to the high temperature parch of doing pot with 70～80 ℃ make.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365358A (en) * | 2014-11-18 | 2015-02-25 | 广西大学 | Simple method for subprostrate sophora manual bagging and breeding |
| CN104737770A (en) * | 2015-04-22 | 2015-07-01 | 詹月星 | Cultivating method for intermediate propagation of tetrastigma planicaule |
| CN105409486A (en) * | 2014-09-12 | 2016-03-23 | 紫云自治县燎原中药材产业化种植专业合作社 | Seed breeding method for Sophora Subprostrata |
| CN105409487A (en) * | 2014-09-12 | 2016-03-23 | 紫云自治县燎原中药材产业化种植专业合作社 | Cuttage seedling propagation method for Sophora Subprostrata |
| CN107624534A (en) * | 2017-10-17 | 2018-01-26 | 伊犁师范学院 | A kind of method that Sophora alopecuroide seedling is efficiently bred using rhizome |
| CN108834595A (en) * | 2018-07-18 | 2018-11-20 | 安龙县森圩种养农民专业合作社 | A kind of wild subprostrate sophora cuttage breeding method |
| CN109042184A (en) * | 2018-07-16 | 2018-12-21 | 临沧茂祥种植有限公司 | A kind of wild thin colored root artificial domesticating cultivation method |
| CN112119828A (en) * | 2020-10-10 | 2020-12-25 | 紫云县惠农种植养殖农民专业合作社 | Planting method of subprostrate sophora |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409486A (en) * | 2014-09-12 | 2016-03-23 | 紫云自治县燎原中药材产业化种植专业合作社 | Seed breeding method for Sophora Subprostrata |
| CN105409487A (en) * | 2014-09-12 | 2016-03-23 | 紫云自治县燎原中药材产业化种植专业合作社 | Cuttage seedling propagation method for Sophora Subprostrata |
| CN105409487B (en) * | 2014-09-12 | 2018-08-24 | 紫云自治县燎原中药材产业化种植专业合作社 | A kind of cuttage and seedling culture method of subprostrate sophora |
| CN105409486B (en) * | 2014-09-12 | 2019-02-26 | 紫云自治县燎原中药材产业化种植专业合作社 | A kind of seed growing method of subprostrate sophora |
| CN104365358A (en) * | 2014-11-18 | 2015-02-25 | 广西大学 | Simple method for subprostrate sophora manual bagging and breeding |
| CN104737770A (en) * | 2015-04-22 | 2015-07-01 | 詹月星 | Cultivating method for intermediate propagation of tetrastigma planicaule |
| CN107624534A (en) * | 2017-10-17 | 2018-01-26 | 伊犁师范学院 | A kind of method that Sophora alopecuroide seedling is efficiently bred using rhizome |
| CN109042184A (en) * | 2018-07-16 | 2018-12-21 | 临沧茂祥种植有限公司 | A kind of wild thin colored root artificial domesticating cultivation method |
| CN108834595A (en) * | 2018-07-18 | 2018-11-20 | 安龙县森圩种养农民专业合作社 | A kind of wild subprostrate sophora cuttage breeding method |
| CN112119828A (en) * | 2020-10-10 | 2020-12-25 | 紫云县惠农种植养殖农民专业合作社 | Planting method of subprostrate sophora |
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