CN108834595A - A kind of wild subprostrate sophora cuttage breeding method - Google Patents
A kind of wild subprostrate sophora cuttage breeding method Download PDFInfo
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- CN108834595A CN108834595A CN201810790627.XA CN201810790627A CN108834595A CN 108834595 A CN108834595 A CN 108834595A CN 201810790627 A CN201810790627 A CN 201810790627A CN 108834595 A CN108834595 A CN 108834595A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The present invention relates to wild subprostrate sophora mating systems, more particularly to a kind of wild subprostrate sophora cuttage breeding method, by being impregnated with auxin solution to cutting, auxin in hormone solution, gibberellin, the basic element of cell division can promote the development in advance of plant, improve the speed of growth, reduce nursery early period and growth of seedling time in later period, before nursery, lead to steam to seedbed and matrix to kill virus, steam can be good at killing bacterium, new pollution will not be brought, very good solution subprostrate sophora expands numerous problem easy to pollute, medium of seedling bed is laid with selection layering and is laid with matrix, i.e. bottom is laid with organic substrate, the mode of upper layer laying inorganic matrix, seedlings plugging is not only contributed in this way to take root, the later period is also facilitated to carry out de- fertilizer.
Description
Technical field
The present invention relates to wild subprostrate sophora mating systems, and in particular to a kind of wild subprostrate sophora cuttage breeding method.
Background technique
Subprostrate sophora is Dicotyledoneae pulse family Euchrest plant also known as sophora tonkinensis Gapnep, root of subprostrate sophora, lima bean lotus, is mainly divided
It is distributed in Guangxi, Guizhou, Yunnan.Subprostrate sophora is the shrub of high 1~2m, is born in the stone in subtropical zone or temperate zone 1000~2000m of height above sea level
It in limestone mountainous region or rock seam, is scattered about like the stars in cheuch small stream side evergreen broad-leaved hayashishita, likes dark and damp, humus environment abundant, it is usually raw
It is longer than in red soil or yellow earth, pH value is 4.5~5.5.Therefore, the artificial culture of wild subprostrate sophora is not easy to realize.Subprostrate sophora is done
Dry root and rhizome be common Chinese medicine, can Qinghuo Jiedu, swelling and pain relieving, be usually used in throat swelling and aching of gum, cough with lung heat's polydipsia, Huang
Subcutaneous ulcer, constipation with heat retention.Sophorae subprostratae radix extract also has the effects that antitumor, resisting pathogenic microbes, and medical value with higher is led
It causes people excessively to acquire subprostrate sophora, declines wild resource sharply.It was promulgated at 1997 and 1999《Special-protection-by-the-State
Wild plant register》In, subprostrate sophora be included for second class protection kind (《Chinese Plants Red Data Book》(first) is included in three-level
Protection kind), it is seen that its stock number in the whole nation is also more rare.Therefore, subprostrate sophora is cultivated, especially wild subprostrate sophora has
Great economic value and important meaning.
The modes of reproduction of subprostrate sophora is usually seminal propagation, but because its germinating power is low, and growth is slower, and fertility is low,
Therefore in the prior art frequently with method for tissue culture, to achieve the purpose that quickly to breed subprostrate sophora.But the kind of wild subprostrate sophora
The germination rate of son is very low, and higher to germination environmental requirement, keeps seed-seedling conversion ratio also very low, in addition its slow growth,
Seriously constrain the expansion of population.Especially after people lead to the destruction in subprostrate sophora habitat to the destruction of forest, subprostrate sophora
Though fertility and viability, which drop to, to be grown reluctantly, hardly reproducible stage.So general subprostrate sophora group
Training propagation method is not necessarily suitable the culture of wild subprostrate sophora.
The plantation for wanting popularization subprostrate sophora needs to find a kind of numerous mode of expansion easy to operate, at low cost and high breeding potential,
In this process, it is also necessary to overcome the problems, such as that subprostrate sophora cutting propagation is easy to pollute.
Summary of the invention
The object of the present invention is to provide a kind of wild subprostrate sophora cuttage breeding methods, impregnate subprostrate sophora cutting by hormone,
The mode in building sterilization seedbed and matrix, to find a kind of inexpensive, numerous mode of the simple subprostrate sophora expansion of operating method.
A kind of wild subprostrate sophora cuttage breeding method includes the following steps：
1) cutting：Annual wild subprostrate sophora plant is selected, plant is cut to the stem with bud of 15~20cm, every section
4~5 buds do cutting, spare；
2) hormone is prepared：It takes one to clean, dry, sterilization container, heteroauxin, 6- furans adenine phosphate, benzyl gland is added
Purine and water are mixed to prepare auxin solution under room temperature；
3) it impregnates：Cutting is placed in auxin solution and carries out soak at room temperature；
4) seedbed：Select seedbed, by seedbed go after cutting weeds and being smooth, be laid on the ground one layer of length and width be 20m ×
The common brick of 10m, makes seedbed be above the ground level, and in seedbed, surrounding inlays out seedbed with plank, takes sunshade around seedbed；
5) matrix：Matrix is laid in seedbed, matrix bottom is leaf mould, and upper layer is sea sand, after being laid with matrix, by seedling
Bed is all covered with high temperature cloth, is imported steam, is carried out antivirus processing；
6) cuttage culture：Will by the cutting that auxin impregnates by the cuttage of 10cm × 10cm line space to sterilized
In processed seedbed, heat preservation, moisturizing, culture obtain wild subprostrate sophora seedling；
7) it transplants：Seedling replanting is gone to big Tanaka, seeding row spacing 30cm × 40cm transverse direction Furrow sowing, transplanting are pressed when transplanting
Preceding application 3cm thickness base fertilizer；
8) field is managed and protected：After Seeding planting, tillage and weeding 1 time every other month, rainy season pays attention to draining, in order to avoid rotten；
9) it harvests：Autumn can carry out subprostrate sophora stem tuber harvesting after transplanting 2~3 years.
Preferably, in step 2), hormone solution be heteroauxin, 6- furans adenine phosphate, benzyladenine and water according to
2：1：1：The mixing of 500 ratios.
Preferably, in step 3), cutting 2/3~3/4, which is inserted into hormone solution, impregnates, and soaking time is 4~6h.
Preferably, in step 4), seedbed height is in 18~20cm.
Preferably, in step 5), leaf mould and sea sand the thickness ratio in seedbed are 4：1, vapor (steam) temperature at 100~110 DEG C,
1~2h of disinfecting time.
Preferably, in step 6), stromal surface is exposed in cutting 1/4~1/3, and nursery bed temperature maintains 20~25 DEG C, humidity
60~70% are maintained, primary every 2~3d watering, incubation time is 25~30d.
Preferably, in step 7), base fertilizer be with 20 parts of chicken manure, 25 parts of cow dung, 15 parts of plant ash, 10 parts of stalk at normal temperature
Ferment organic fertilizer made of 5d.
The beneficial effects of the invention are as follows：
1) a kind of wild subprostrate sophora cuttage breeding method of the present invention, impregnates cutting with auxin solution,
Auxin, gibberellin, the basic element of cell division in hormone solution can promote the development in advance of plant, improve the speed of growth, reduce
Nursery early period and growth of seedling time in later period.
2) a kind of wild subprostrate sophora cuttage breeding method of the present invention leads to steam to seedbed and matrix and kills before nursery
Poison, steam can be good at killing bacterium, will not bring new pollution, and very good solution subprostrate sophora expands numerous easy to pollute ask
Topic.
3) a kind of wild subprostrate sophora cuttage breeding method of the present invention, medium of seedling bed are laid with selection layering and are laid with matrix, i.e. bottom
Layer is laid with organic substrate, and upper layer is laid with the mode of inorganic matrix, not only contributes to seedlings plugging in this way and take root, also the later period is facilitated to be taken off
Fertilizer.
Specific embodiment
The present invention is further described in detail With reference to embodiment, but technical solution provided by the invention
It not only include the content showed in embodiment.
Embodiment 1
A kind of wild subprostrate sophora cuttage breeding method is present embodiments provided, detailed process is as follows：
1, cutting：Annual wild subprostrate sophora plant is selected, plant is cut to the stem with bud of 15cm, every section of 4 buds,
Cutting is done, it is spare；
2, hormone is prepared：One is taken to clean, dry, sterilization container, heteroauxin, 6- furans adenine phosphate, benzyladenine
With water according to 2：1：1：500 ratios mix at normal temperature, and auxin solution is made；
3, it impregnates：Cutting is placed in auxin solution and carries out soak at room temperature, cutting 2/3 is inserted into hormone solution
Middle immersion, soaking time 4h；
4, seedbed：Select seedbed, by seedbed go after cutting weeds and being smooth, be laid on the ground one layer of length and width be 20m ×
The common brick of 10m, makes seedbed be above the ground level, and in seedbed, surrounding inlays out seedbed with plank, and seedbed height takes screening around 18cm, seedbed
Yang Peng；
5, matrix：Matrix is laid in seedbed, matrix bottom is leaf mould, and upper layer is sea sand, and leaf mould and sea sand are in seedling
Thickness ratio is 4 in bed：1, after being laid with matrix, seedbed is all covered with high temperature cloth, steam is imported, carries out antivirus processing, is steamed
Stripping temperature is at 100 DEG C, disinfecting time 1h；
6, cuttage culture：Will by the cutting that auxin impregnates by the cuttage of 10cm × 10cm line space to sterilized
In processed seedbed, stromal surface is exposed in cutting 1/4, and nursery bed temperature maintains 20 DEG C, and humidity maintains 60%, pours every 2d
Water is primary, and incubation time is that 25d obtains wild subprostrate sophora seedling；
7, it transplants：Seedling replanting is gone to big Tanaka, seeding row spacing 30cm × 40cm transverse direction Furrow sowing, transplanting are pressed when transplanting
Preceding application 3cm thickness base fertilizer, base fertilizer be ferment 5d at normal temperature with 20 parts of chicken manure, 25 parts of cow dung, 15 parts of plant ash, 10 parts of stalk and
At organic fertilizer；
8, field is managed and protected：After Seeding planting, tillage and weeding 1 time every other month, rainy season pays attention to draining, in order to avoid rotten；
9, it harvests：Autumn can carry out subprostrate sophora stem tuber harvesting after transplanting 2 years.
Embodiment 2
A kind of wild subprostrate sophora cuttage breeding method is present embodiments provided, detailed process is as follows：
1, cutting：Annual wild subprostrate sophora plant is selected, plant is cut to the stem with bud of 18cm, every section of 4 buds,
Cutting is done, it is spare；
2, hormone is prepared：One is taken to clean, dry, sterilization container, heteroauxin, 6- furans adenine phosphate, benzyladenine
With water according to 2：1：1：500 ratios mix at normal temperature, and auxin solution is made；
3, it impregnates：Cutting is placed in auxin solution and carries out soak at room temperature, cutting 2/3 is inserted into hormone solution
Middle immersion, soaking time 5h；
4, seedbed：Select seedbed, by seedbed go after cutting weeds and being smooth, be laid on the ground one layer of length and width be 20m ×
The common brick of 10m, makes seedbed be above the ground level, and in seedbed, surrounding inlays out seedbed with plank, and seedbed height takes screening around 19cm, seedbed
Yang Peng；
5, matrix：Matrix is laid in seedbed, matrix bottom is leaf mould, and upper layer is sea sand, and leaf mould and sea sand are in seedling
Thickness ratio is 4 in bed：1, after being laid with matrix, seedbed is all covered with high temperature cloth, steam is imported, carries out antivirus processing, is steamed
Stripping temperature is at 105 DEG C, disinfecting time 2h；
6, cuttage culture：Will by the cutting that auxin impregnates by the cuttage of 10cm × 10cm line space to sterilized
In processed seedbed, stromal surface is exposed in cutting 1/4, and nursery bed temperature maintains 23 DEG C, and humidity maintains 65%, pours every 2d
Water is primary, and incubation time is that 28d obtains wild subprostrate sophora seedling；
7, it transplants：Seedling replanting is gone to big Tanaka, seeding row spacing 30cm × 40cm transverse direction Furrow sowing, transplanting are pressed when transplanting
Preceding application 3cm thickness base fertilizer, base fertilizer be ferment 5d at normal temperature with 20 parts of chicken manure, 25 parts of cow dung, 15 parts of plant ash, 10 parts of stalk and
At organic fertilizer；
8, field is managed and protected：After Seeding planting, tillage and weeding 1 time every other month, rainy season pays attention to draining, in order to avoid rotten；
9, it harvests：Autumn can carry out subprostrate sophora stem tuber harvesting after transplanting 2 years.
Embodiment 3
A kind of wild subprostrate sophora cuttage breeding method is present embodiments provided, detailed process is as follows：
1, cutting：Annual wild subprostrate sophora plant is selected, plant is cut to the stem with bud of 20cm, every section of 5 buds,
Cutting is done, it is spare；
2, hormone is prepared：One is taken to clean, dry, sterilization container, heteroauxin, 6- furans adenine phosphate, benzyladenine
With water according to 2：1：1：500 ratios mix at normal temperature, and auxin solution is made；
3, it impregnates：Cutting is placed in auxin solution and carries out soak at room temperature, cutting 3/4 is inserted into hormone solution
Middle immersion, soaking time 6h；
4, seedbed：Select seedbed, by seedbed go after cutting weeds and being smooth, be laid on the ground one layer of length and width be 20m ×
The common brick of 10m, makes seedbed be above the ground level, and in seedbed, surrounding inlays out seedbed with plank, and seedbed height takes screening around 20cm, seedbed
Yang Peng；
5, matrix：Matrix is laid in seedbed, matrix bottom is leaf mould, and upper layer is sea sand, and leaf mould and sea sand are in seedling
Thickness ratio is 4 in bed：1, after being laid with matrix, seedbed is all covered with high temperature cloth, steam is imported, carries out antivirus processing, is steamed
Stripping temperature is at 110 DEG C, disinfecting time 2h；
6, cuttage culture：Will by the cutting that auxin impregnates by the cuttage of 10cm × 10cm line space to sterilized
In processed seedbed, stromal surface is exposed in cutting 1/3, and nursery bed temperature maintains 25 DEG C, and humidity maintains 70%, pours every 3d
Water is primary, and incubation time is that 30d obtains wild subprostrate sophora seedling；
7, it transplants：Seedling replanting is gone to big Tanaka, seeding row spacing 30cm × 40cm transverse direction Furrow sowing, transplanting are pressed when transplanting
Preceding application 3cm thickness base fertilizer, base fertilizer be ferment 5d at normal temperature with 20 parts of chicken manure, 25 parts of cow dung, 15 parts of plant ash, 10 parts of stalk and
At organic fertilizer；
8, field is managed and protected：After Seeding planting, tillage and weeding 1 time every other month, rainy season pays attention to draining, in order to avoid rotten；
9, it harvests：Autumn can carry out subprostrate sophora stem tuber harvesting after transplanting 3 years.
Claims (7)
1. a kind of wild subprostrate sophora cuttage breeding method, which is characterized in that include the following steps：
1) cutting：Annual wild subprostrate sophora plant is selected, plant is cut to the stem with bud of 15~20cm, every section 4~5
Bud does cutting, spare；
2) hormone is prepared：It takes one to clean, dry, sterilization container, heteroauxin, 6- furans adenine phosphate, benzyladenine is added
And water, auxin solution is mixed to prepare under room temperature；
3) it impregnates：Cutting is placed in auxin solution and carries out soak at room temperature；
4) seedbed：With selecting seedbed, it by seedbed goes after cutting weeds and being smooth, being laid with one layer of length and width on the ground is 20m × 10m's
Common brick makes seedbed be above the ground level, and in seedbed, surrounding inlays out seedbed with plank, takes sunshade around seedbed；
5) matrix：Matrix is laid in seedbed, matrix bottom is leaf mould, and upper layer is sea sand, after being laid with matrix, seedbed is complete
Portion is covered with high temperature cloth, is imported steam, is carried out antivirus processing；
6) cuttage culture：Will by the cutting that auxin impregnates by the cuttage of 10cm × 10cm line space to sterile-processed
In the seedbed crossed, heat preservation, moisturizing, culture obtain wild subprostrate sophora seedling；
7) it transplants：Seedling replanting is gone to big Tanaka, seeding row spacing 30cm × 40cm transverse direction Furrow sowing is pressed when transplanting, is applied before transplanting
Add 3cm thickness base fertilizer；
8) field is managed and protected：After Seeding planting, tillage and weeding 1 time every other month, rainy season pays attention to draining, in order to avoid rotten；
9) it harvests：Autumn can carry out subprostrate sophora stem tuber harvesting after transplanting 2~3 years.
2. a kind of wild subprostrate sophora cuttage breeding method as described in claim 1, which is characterized in that the step 2) hormone is molten
Liquid is heteroauxin, 6- furans adenine phosphate, benzyladenine and water according to 2：1：1：The mixing of 500 ratios.
3. a kind of wild subprostrate sophora cuttage breeding method as described in claim 1, which is characterized in that the step 3) cutting 2/
3~3/4 are inserted into hormone solution and impregnate, and soaking time is 4~6h.
4. a kind of wild subprostrate sophora cuttage breeding method as described in claim 1, which is characterized in that the step 4) seedbed is high
Degree is in 18~20cm.
5. a kind of wild subprostrate sophora cuttage breeding method as described in claim 1, which is characterized in that the step 5) leaf mould
Thickness ratio is 4 in seedbed with sea sand：1, vapor (steam) temperature is at 100~110 DEG C, 1~2h of disinfecting time.
6. a kind of wild subprostrate sophora cuttage breeding method as described in claim 1, which is characterized in that the step 6) cutting 1/
4~1/3 expose stromal surface, and nursery bed temperature maintains 20~25 DEG C, and humidity maintains 60~70%, every 2~3d watering one
Secondary, incubation time is 25~30d.
7. a kind of wild subprostrate sophora cuttage breeding method as described in claim 1, which is characterized in that the step 7) base fertilizer is
It is fermented at normal temperature organic fertilizer made of 5d with 20 parts of chicken manure, 25 parts of cow dung, 15 parts of plant ash, 10 parts of stalk.
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|---|---|---|---|---|
| CN115462290A (en) * | 2022-08-18 | 2022-12-13 | 昌江广凌农业科技有限公司 | Sweet potato seedling cultivation method capable of shortening growth cycle |
| CN115462290B (en) * | 2022-08-18 | 2023-09-08 | 昌江广凌农业科技有限公司 | Sweet potato seedling cultivation method capable of shortening growth period |
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