CN104170738A - Rapid propagation method of acer ginnala maxim. - Google Patents
Rapid propagation method of acer ginnala maxim. Download PDFInfo
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- CN104170738A CN104170738A CN201410449536.1A CN201410449536A CN104170738A CN 104170738 A CN104170738 A CN 104170738A CN 201410449536 A CN201410449536 A CN 201410449536A CN 104170738 A CN104170738 A CN 104170738A
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Abstract
The invention disclosees a rapid propagation method of acer ginnala maxim. The rapid propagation method comprises the steps of sterile seedling obtaining, cluster bud propagation, rooting culture and hardened-seedling transplantation. The acer ginnala maxim prepared by using the method has the advantages of high growth speed, short growth cycle, high propagation coefficient and high survival rate, a plurality of seedlings of acer ginnala maxim can be obtained within a short period of time, and a basis is provided for the development of the medicinal value of the acer ginnala maxim.
Description
Technical field
The present invention relates to the quick-breeding method that herb mixtures tea maple tissue is cultivated, belong to plant technology field.
Background technology
Herb mixtures tea maple,
acer ginnala Maxim., deciduous tree, has another name called Amur maple, mulberry young tea leaves, chicken tea and is distributed in Hubei, Hunan, Guangdong, the ground such as Guangxi.Weak positive, cold-resistant, resistance to dry, avoid waterlogging, anti-flue dust, has very high medical value and practicality, and bark, leaf and fruit all contain tannin, can obtain through refining tannin extract, can be again black dyes.The fiber of bark can be made the raw material of synthetic cotton and papermaking.Tender leaf can replace tealeaves with being beverage after drying, and has the effect reducing blood pressure, and seed oil expression, can be in order to manufacture soap.The modes of reproduction of Chinese medicine is seminal propagation at present, and the breeding cycle is long, and growth conditions requires high, is unfavorable for reaching large-scale production, and the breeding that tissue culture technology is applied to herb mixtures tea maple there is no research both at home and abroad.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for quickly breeding of a kind of herb mixtures tea maple, by the prepared herb mixtures tea maple fast growth of the method, growth cycle is short, growth coefficient is high, survival rate is high, can obtain in a short time a large amount of herb mixtures tea maple seedling, for the exploitation of herb mixtures tea maple medical value provides basis.
Technical problem to be solved by this invention realizes by following scheme:
Get herb mixtures tea maple seed, soak 3min in bleaching powder, soak 10h in water, 5h changes water one time, and on superclean bench, 75% alcohol is processed 30s, and mercury chloride is processed 16min, aseptic water washing 5-7 time.In the herb mixtures tea maple seed access MS+8mg/L kanamycin medium that sterilization treatment is crossed, cultivate, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the aseptic bud seedling growing out, puts into proliferated culture medium MS+IAA0.3 mg/L+ABA1mg/L+ZT2mg/L+4mg/LGA
3+ 50mg/L glutathione, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, the Multiple Buds that propagation cultivates is out put into root media White+NAA0.1mg/L+ lanthanum nitrate 1mg/L, and test-tube plantlet is taken out from blake bottle, washes away the medium of root, carbendazim soaks root, plant in perlite: in the matrix of humus soil=1:1, cultivate, cultivate after 20d, be transplanted into and in rural area soil, carry out land for growing field crops plantation.
Adopting the herb mixtures tea maple survival rate prepared of the present invention is 94%, and the cycle is short, and output is high, and less energy consumption pollutes littlely, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiment.
Embodiment 1
Get herb mixtures tea maple seed, soak 3min in bleaching powder, soak 10h in water, 5h changes water one time, and on superclean bench, 75% alcohol is processed 30s, and mercury chloride is processed 16min, aseptic water washing 5-7 time.In the herb mixtures tea maple seed access MS+8mg/L kanamycin medium that sterilization treatment is crossed, cultivate, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the aseptic bud seedling growing out, puts into proliferated culture medium MS+IAA0.2mg/L+ABA0.5mg/L+ZT1.5mg/L+4mg/LGA
3+ 50mg/L glutathione, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, the Multiple Buds that propagation cultivates is out put into root media White+NAA0.1mg/L+ lanthanum nitrate 0.5mg/L, and the herb mixtures tea maple test-tube plantlet after taking root is first transplanted to greenhouse, then be transplanted to land for growing field crops and carry out hardening cultivation, herb mixtures tea maple survival rate is 90%.
Embodiment 2
Get herb mixtures tea maple seed, soak 3min in bleaching powder, soak 10h in water, 5h changes water one time, and on superclean bench, 75% alcohol is processed 30s, and mercury chloride is processed 16min, aseptic water washing 5-7 time.In the herb mixtures tea maple seed access MS+8mg/L kanamycin medium that sterilization treatment is crossed, cultivate, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the aseptic bud seedling growing out, puts into proliferated culture medium MS+IAA0.5 mg/L+ABA1.5mg/L+ZT3mg/L+4mg/LGA
3+ 50mg/L glutathione, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, the Multiple Buds that propagation cultivates is out put into root media White+NAA0.1mg/L+ lanthanum nitrate 1.5mg/L, and the herb mixtures tea maple test-tube plantlet after taking root is first transplanted to greenhouse, then be transplanted to land for growing field crops and carry out hardening cultivation, herb mixtures tea maple survival rate is 91%.
Embodiment 3
Get herb mixtures tea maple seed, soak 3min in bleaching powder, soak 10h in water, 5h changes water one time, and on superclean bench, 75% alcohol is processed 30s, and mercury chloride is processed 16min, aseptic water washing 5-7 time.In the herb mixtures tea maple seed access MS+8mg/L kanamycin medium that sterilization treatment is crossed, cultivate additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the aseptic bud seedling growing out, puts into proliferated culture medium MS+IAA0.2mg/L+ABA1mg/L+ZT2mg/L+4mg/LGA
3+ 50mg/L glutathione, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, the Multiple Buds that propagation cultivates is out put into root media White+NAA0.1mg/L+ lanthanum nitrate 0.5mg/L, and the herb mixtures tea maple test-tube plantlet after taking root is first transplanted to greenhouse, then be transplanted to land for growing field crops and carry out hardening cultivation, herb mixtures tea maple survival rate is 92%.
Embodiment 4
Get herb mixtures tea maple seed, soak 3min in bleaching powder, soak 10h in water, 5h changes water one time, and on superclean bench, 75% alcohol is processed 30s, and mercury chloride is processed 16min, aseptic water washing 5-7 time.In the herb mixtures tea maple seed access MS+8mg/L kanamycin medium that sterilization treatment is crossed, cultivate, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the aseptic bud seedling growing out, puts into proliferated culture medium MS+IAA0.5 mg/L+ABA1mg/L+ZT3mg/L+4mg/LGA
3+ 50mg/L glutathione, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, the Multiple Buds that propagation cultivates is out put into root media White+NAA0.1mg/L+ lanthanum nitrate 0.5mg/L, and the herb mixtures tea maple test-tube plantlet after taking root is first transplanted to greenhouse, then be transplanted to land for growing field crops and carry out hardening cultivation, herb mixtures tea maple survival rate is 93%.
Claims (7)
1. a method for quickly breeding for herb mixtures tea maple, comprises the sterilization of explant, acquisition, adventitious buds proliferation, culture of rootage and the acclimatization and transplants of aseptic seedling, and its key step is as follows:
(1) get the seed of herb mixtures tea maple, it is carried out to sterilization treatment;
(2) in the herb mixtures tea maple seed access MS+8mg/L kanamycin medium of step (1) sterilization treatment being crossed, cultivate additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature;
(3) get the aseptic bud seedling that step (2) grows out, put into proliferated culture medium MS+IAA0.2-0.5 mg/L+ABA0.5-1.5mg/L+ZT1.5-3mg/L+4mg/LGA
3+ 50mg/L glutathione, additional saccharose 30g/L, agar 6g/L, PH5.8-5.9, illumination 1000lx;
(4) getting step (3) breeds the Multiple Buds of cultivating out and puts into root media White+NAA0.1mg/L+ lanthanum nitrate 0.5-1.5mg/L;
(5) the herb mixtures tea maple test-tube plantlet of getting after step (4) is taken root is first transplanted to greenhouse, is then transplanted to land for growing field crops and carries out hardening cultivation.
2. according to the method for quickly breeding of a kind of herb mixtures tea maple claimed in claim 1, it is characterized in that: described in step (1), the sterilization treatment of herb mixtures tea maple seed is, get herb mixtures tea maple seed, in bleaching powder, soak 3min, in water, soak 10h, 5h changes water one time, and on superclean bench, 75% alcohol is processed 30s, mercury chloride is processed 16min, aseptic water washing 5-7 time.
3. according to the method for quickly breeding of a kind of herb mixtures tea maple claimed in claim 1, it is characterized in that: in the cultivation of step (2) Aseptic Seedling Growth, added kanamycin 8mg/L, be used for eliminating endophyte.
4. according to the method for quickly breeding of a kind of herb mixtures tea maple claimed in claim 1, it is characterized in that: in the proliferated culture medium in step (3), added 4mg/LGA
3with 50mg/L glutathione, glutathione can prevent from occurring in breeding browning, GA
3can promote the propagation of seedling, GA
3medium after need are subject to sterilization is cooled to 60 DEG C to carry out below filtration sterilization.
5. according to the method for quickly breeding of a kind of herb mixtures tea maple claimed in claim 1, it is characterized in that: step has added lanthanum nitrate 0.5-1.5mg/L in (4), and rare-earth substance can promote taking root of plant.
6. according to the method for quickly breeding of a kind of herb mixtures tea maple claimed in claim 1, it is characterized in that: the culture of rootage condition in step (4) is sucrose 20g/L agar 5g/L, PH5.8-5.9, illumination 2000lx, humidity 60-70%, photophase 14h.
7. according to the method for quickly breeding of a kind of herb mixtures tea maple claimed in claim 1, it is characterized in that: in step (5), the hardening cultural method of herb mixtures tea maple for to take out test-tube plantlet from blake bottle, wash away the medium of root, carbendazim soaks root, plant in perlite: in the matrix of humus soil=1:1, cultivate, cultivate after 20 days, be transplanted into and in rural area soil, carry out land for growing field crops plantation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410449536.1A CN104170738A (en) | 2014-09-04 | 2014-09-04 | Rapid propagation method of acer ginnala maxim. |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410449536.1A CN104170738A (en) | 2014-09-04 | 2014-09-04 | Rapid propagation method of acer ginnala maxim. |
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| CN104170738A true CN104170738A (en) | 2014-12-03 |
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| CN201410449536.1A Pending CN104170738A (en) | 2014-09-04 | 2014-09-04 | Rapid propagation method of acer ginnala maxim. |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737905A (en) * | 2015-02-14 | 2015-07-01 | 涂湘炎 | Millettia specisoa tissue culture rooting method |
| CN108077070A (en) * | 2016-11-21 | 2018-05-29 | 四川七彩林业开发有限公司 | A kind of maple tissue cultures culture medium and cultural method |
-
2014
- 2014-09-04 CN CN201410449536.1A patent/CN104170738A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737905A (en) * | 2015-02-14 | 2015-07-01 | 涂湘炎 | Millettia specisoa tissue culture rooting method |
| CN108077070A (en) * | 2016-11-21 | 2018-05-29 | 四川七彩林业开发有限公司 | A kind of maple tissue cultures culture medium and cultural method |
| CN108077070B (en) * | 2016-11-21 | 2020-08-21 | 四川七彩林科股份有限公司 | Culture medium and culture method for maple tissue culture |
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Application publication date: 20141203 |
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