CN104839023A - Method for cultivating dendrobium officinale - Google Patents
Method for cultivating dendrobium officinale Download PDFInfo
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- CN104839023A CN104839023A CN201510238468.9A CN201510238468A CN104839023A CN 104839023 A CN104839023 A CN 104839023A CN 201510238468 A CN201510238468 A CN 201510238468A CN 104839023 A CN104839023 A CN 104839023A
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Abstract
The invention discloses a method for cultivating dendrobium officinale, which comprises the following steps: tissue-cultured seedlings of dendrobium officinale are subjected to a disinfection procedure, namely the tissue-cultured seedlings are placed into a culture medium for seed germination culture, wherein the culture medium contains the following components: an MS basic culture medium, 0.4mg/L NAA, 0.5mg/L 1-naphthylacetic acid, 30g/L banana juice, 0.9mg/L paclobutrazol, 45g/L potato juice and 7g/L agaragar, and the pH value of the culture medium is 5.5-6.5; change-over culture of the tissue-cultured seedlings of dendrobium officinale is carried out, namely, tissue-cultured seedlings of dendrobium officinale, which are 1-3cm in plant height and have 2-3 true leaves and a completely developed root system, in a tissue culture room are selected, are changed onto the culture medium prepared in the first step on a super clean worktable, and are placed in a tissue culture room for culture; two months later, the tissue-cultured seedlings are developed into middle seedlings, which are 2.0-3.5cm in height, and the middle seedlings are changed into the same culture medium; two months later, big seedlings which are 5-6cm in height and 1-1.5cm in root length are cultured, and the big seedlings can be taken out of a bottle for plantlet hardening and are then transplanted. Compared with conventional seedling expanding propagation, the seedling expanding propagation speed is improved by 50%, the transplant survival rate is improved to more than 95%, and the yield is improved by more than 40%.
Description
Technical field
The present invention relates to a kind of cultural method of dendrobium candidum, belong to cultivation method technical field.
Background technology
The stem of noble dendrobium is the orchid family (Orchidaceae) Dendrobium (Dendrobium Sw.) plant, and whole world the orchid family about has 500 genus, kind more than 15000; Dendrobium is one of genus maximum in the orchid family.China's the orchid family about has 150 genus, 1000 kinds, is mainly distributed in provinces and regions on the south the Qinling Mountains and the Yangtze river basin; The most of kind of Dendrobium Sw all integrated distribution, between north latitude 15 ° 30 ' ~ 25 ° 12 ', and is prolonged kind more northwards and is then reduced gradually.China's dendrobium candidum is mainly distributed in the ground such as Zhejiang, Guangxi, Hunan, Yunnan, Guizhou.
Harshness due to Growth of Dendrobium candidum environment, artificial cultivation dendrobium candidum must capture the technical barrier of stable variety, prevents the degeneration of iron sheet kind.Therefore tame dendrobium candidum is generally divided into two kinds, and a kind of is the place of production, source at iron sheet, namely traditional Chinese medicine " tunnel " place of production of being particular about, as Yunnan, zhejiang and other places, rely on natural control environment, plant with the form kind of booth, its output, quality are all comparatively subject to local environment restriction.Another kind of then be the mode by imitative wild simulation, a series of facilities and equipment is utilized to simulate the environmental condition such as matrix, temperature, illumination, air quality, humidity of optimum Growth of Dendrobium candidum, formed " microclimate " that specialty is unique, again by modern agriculture management means disease and pest control, guarantee the stable of the variety and quality of dendrobium candidum.
With regard to the selection in officinal dendrobium stem plantation area, inventor thinks and according to the biological property of dendrobium candidum, should take into full account the natural causes (generally selecting the area having dendrobium officinale to distribute) such as the illumination in place, temp. and humidity, ventilation.In order to obtain the dendrobium candidum of high yield, high-quality, standardized planting, intensive cultivating must be accomplished, the growing environment that artificial creation one is superior, making planting conditions substantially close to its wild environmental weather conditions, to meet its growth needs.Generally in greenhouse, seedbed is planted, such several factors can carry out Artificial Control.
Summary of the invention
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Object of the present invention carrys out specific implementation by the following technical programs:
A cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 5.5 ~ 6.5 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 1 ~ 3cm, grow 2 ~ 3 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 25 ± 5 DEG C, the tissue culture room of intensity of illumination 2000 ~ 3000Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 2.0 ~ 3.5cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 5 ~ 6cm after 2 months, seedlings that root length is 1 ~ 1.5cm, seedlings can practice seedling, transplanting by bottle outlet.
Further, NaOH aqueous solution adjust ph to 5.5 ~ 6.5 of described medium 0.1mol/L.
Further, the illumination of the tissue culture room in described step 2 is 12h/12h.
Further, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Embodiment
First it may be noted that embodiments of the invention only disclose preferred embodiment several, not should be appreciated that paired restriction of the invention process, protection scope of the present invention is still as the criterion with the content disclosed in claims.
Embodiment one
A cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 5.5 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 1cm, grow 2 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 20 DEG C, the tissue culture room of intensity of illumination 2000Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 2.0cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 5cm, the long seedlings for 1cm of root after 2 months, seedlings can practice seedling, transplanting by bottle outlet.
Further, the NaOH aqueous solution adjust ph to 5.5 of described medium 0.1mol/L.
Further, the illumination of the tissue culture room in described step 2 is 12h/12h.
Further, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Embodiment two
A cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 6.5 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 3cm, grow 3 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 30 DEG C, the tissue culture room of intensity of illumination 3000Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 3.5cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 6cm, the long seedlings for 1.5cm of root after 2 months, seedlings can practice seedling, transplanting by bottle outlet.
Further, the NaOH aqueous solution adjust ph to 6.5 of described medium 0.1mol/L.
Further, the illumination of the tissue culture room in described step 2 is 12h/12h.
Further, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Embodiment three
A cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 6 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 2cm, grow 2 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 23 DEG C, the tissue culture room of intensity of illumination 2500Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 3cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 5.5cm, the long seedlings for 1.2cm of root after 2 months, seedlings can practice seedling, transplanting by bottle outlet.
Further, the NaOH aqueous solution adjust ph to 6 of described medium 0.1mol/L.
Further, the illumination of the tissue culture room in described step 2 is 12h/12h.
Further, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Embodiment four
A cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 5.7 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 2.5cm, grow 3 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 25 DEG C, the tissue culture room of intensity of illumination 2800Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 3.2cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 2.6cm, the long seedlings for 1.4cm of root after 2 months, seedlings can practice seedling, transplanting by bottle outlet.
Further, the NaOH aqueous solution adjust ph to 5.7 of described medium 0.1mol/L.
Further, the illumination of the tissue culture room in described step 2 is 12h/12h.
Further, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Embodiment five
A cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 5.7 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 2.3cm, grow 3 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 23 DEG C, the tissue culture room of intensity of illumination 2700Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 3.3cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 5.3cm, the long seedlings for 1.3cm of root after 2 months, seedlings can practice seedling, transplanting by bottle outlet.
Further, the NaOH aqueous solution adjust ph to 5.7 of described medium 0.1mol/L.
Further, the illumination of the tissue culture room in described step 2 is 12h/12h.
Further, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
The present invention proposes a kind of cultural method of dendrobium candidum, compared with traditional seeding propagation, the reproductive speed of seedling improves 50%, and transplanting survival rate brings up to more than 95%, output increased more than 40%.
Finally being also pointed out that any entity or individual use or implement technical scheme of the present invention is all to infringement of the present invention, and any entity or individual, without the permission of the applicant, can not implement this patent separately.And any entity or individual are subject to inspiration of the present invention or implement through simple adjustment, also should think the protection domain of this patent.
Claims (4)
1. a cultural method for dendrobium candidum, is characterized in that, the method comprises:
Step one, by candidum tissue culturing seedling through disinfectant program, put into and consist of: MS minimal medium, 0.4mg/L NAA, 0.5mg/L methyl α-naphthyl acetate, 30g/L bananas juice, 0.9mg/L paclobutrazol, 45g/L murphy juice and 7g/L agar and pH value is in 5.5 ~ 6.5 medium, carry out seed germination cultivation;
The switching of step 2, candidum tissue culturing seedling is cultivated and is chosen plant height in group training room and be 1 ~ 3cm, grow 2 ~ 3 true leaves and the complete candidum tissue culturing seedling of root system development, plantlet in vitro is forwarded to by superclean bench on medium that step one prepares, is placed in that temperature is 25 ± 5 DEG C, the tissue culture room of intensity of illumination 2000 ~ 3000Lx is cultivated;
Step 3, after 2 months, plantlet in vitro develops into the middle seedling of 2.0 ~ 3.5cm, is transferred in same medium by middle seedling again, then carries out stock breeding; Turn out high 5 ~ 6cm after 2 months, seedlings that root length is 1 ~ 1.5cm, seedlings can practice seedling, transplanting by bottle outlet.
2. the method for claim 1, is characterized in that, NaOH aqueous solution adjust ph to 5.5 ~ 6.5 of described medium 0.1mol/L.
3. the method for claim 1, is characterized in that, the illumination of the tissue culture room in described step 2 is 12h/12h.
4. the method for claim 1, is characterized in that, in described step 3, seedlings can practice seedling, transplanting by bottle outlet, specifically comprise:
Seedlings are taken off from culturing rack, moves on to rapidly in the booth of double-layered sunshade net, on the ground equably, ensure that surrounding can not be ventilated, and toward bottle wall specking water when temperature is somewhat high at noon; One deck sunshade net is raised, moisturizing, until the stem of seedling becomes bottle green to transplant after 24 hours.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359983A (en) * | 2015-12-24 | 2016-03-02 | 陕西师范大学 | Dendrobium officinale Kimura et Migo protocorm direct-seeding method |
| CN105993935A (en) * | 2016-04-28 | 2016-10-12 | 陕西师范大学 | Rhizome bletillae tissue culture medium, preparation method and application |
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2015
- 2015-05-12 CN CN201510238468.9A patent/CN104839023A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359983A (en) * | 2015-12-24 | 2016-03-02 | 陕西师范大学 | Dendrobium officinale Kimura et Migo protocorm direct-seeding method |
| CN105993935A (en) * | 2016-04-28 | 2016-10-12 | 陕西师范大学 | Rhizome bletillae tissue culture medium, preparation method and application |
| CN105993935B (en) * | 2016-04-28 | 2018-05-22 | 陕西师范大学 | A kind of bletilla tissue culture medium (TCM), preparation method and application |
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Application publication date: 20150819 |