CN102067817A - Method for quickly cultivating medicinal anectochilus roxburhii - Google Patents
Method for quickly cultivating medicinal anectochilus roxburhii Download PDFInfo
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- CN102067817A CN102067817A CN2009101546375A CN200910154637A CN102067817A CN 102067817 A CN102067817 A CN 102067817A CN 2009101546375 A CN2009101546375 A CN 2009101546375A CN 200910154637 A CN200910154637 A CN 200910154637A CN 102067817 A CN102067817 A CN 102067817A
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Abstract
The invention provides a method for quickly cultivating medicinal anectochilus roxburhii. The method comprises the following steps of: sterilizing wild anectochilus roxburhii serving as an original strain and then removing roots and leaves of plants; truncating segments of terminal buds and lateral buds of the plants and performing explant culture on an original culture medium; inoculating stem segments with the length of 0.5 to 1.0cm of explants in a value-added culture medium, culturing in dark for 10 to 15 days and transferring into light to culture for 40 to 50 days; and finally, inoculating plantlets in refined rooting culture medium to induce rooting, and transplanting to an equal amount of sterilized pearlite, river sand and peat soil substrates. The invention provides a new method for quickly cultivating the anectochilus roxburhii, which is easy and convenient to operate, has readily available raw materials and short reproductive cycle, is suitable for factory production, and has a high economic benefit.
Description
Technical field
The invention provides a kind of new medicinal roxburgh anoectochilus terminal bud method for quickly cultivating, relate to plant and manually produce culture technology.
Technical background
Roxburgh anoectochilus terminal bud (Anectochilus roxburhii) another name Shorthairy Antenoron, gold thread disappear to the marrow, for the orchid family is opened lip Cymbidium (Anectochilus) herbaceos perennial, belong to precious Chinese herbal medicine, it can be used for treating multiple diseases such as tuberculosis hemoptysis, tuberculosis, diabetes, hypertension, deficiency of the kidney, ephritis.Roxburgh anoectochilus terminal bud have " black genseng " laudatory title, has higher viewing and admiring and medical value.All there is distribution in Asian countries such as China, India, Japan, and China mainly is distributed in ground such as Fujian, Guangdong, Yunnan.
Roxburgh anoectochilus terminal bud has high medical value, has the flat liver of cool blood, clearing heat and detoxicating effect, and often eating roxburgh anoectochilus terminal bud has unique effects to cancer-resisting and treatment diabetes, ephritis, hepatitis, children's's cool breeze, venomous snake bite, also has beautification function.Min Xi, people from the south of Fujian Province meet the honored guest or the major holiday all can be used roxburgh anoectochilus terminal bud stewed chicken, duck or rib soup.Be a kind of rare wholefood of people health care with bright product 50-100g every day every day.Roxburgh anoectochilus terminal bud is because its multiple medicinal efficacy that has causes the attention of the world of medicine day by day, among the people being called " refreshing medicine ", Taiwan is called " king of medicine ", according to market survey in January, 2008, and 1000 yuan of fresh sward retail price per kilograms, because roxburgh anoectochilus terminal bud is strict to ecotope, natural propagation and poor growth add that birds have a liking for food and artificial a large amount of the collection, and wild resource reduces day by day, can not satisfy the clinical treatment needs, domestic relevant department is carrying out roxburgh anoectochilus terminal bud and is breeding research fast.
Under the natural situation, roxburgh anoectochilus terminal bud grows slowly, and seed is not had an endosperm, and no germination ability only could promote the seed germination growth under will mycosymbiosis condition, but its germination rate is extremely low, so its resources of natural crude drugs has been on the verge of exhaustion.Adopt methods such as artificial seed breeding, root division, cottage propagation to need the time longer, and reproductive survival rate is not high, the fast breeding that utilizes tissue culture technique to carry out seedling is to solve the tame crux of roxburgh anoectochilus terminal bud, to fully providing the medicine source significant.The present invention adopts method for plant tissue culture, to the bacterial classification initial stage cultivate, explant growth and the strengthening seedling and rooting stage in later stage optimize, and set up a cover rapid propagation system, producing for the roxburgh anoectochilus terminal bud batch production provides guidance.
Summary of the invention
1. new medicinal roxburgh anoectochilus terminal bud method for quickly cultivating, its feature comprises following step:
(1) explant is cultivated
A wild roxburgh anoectochilus terminal bud material part is adopted back back artificial planting a period of time and is sterilized from the mountain, a part is without directly sterilization of plantation.Remove plant root and blade earlier, intercept the sections of its terminal bud and lateral bud, wash 20min with sterile distilled water, 75% alcohol rinsing 30s, mercuric chloride vibration sterilization 10～13min of 0.1% uses aseptic water washing 3～6 times again, is cutting on the superclean bench on terminal bud, the lateral bud access startup medium.Place 15d in the dark after the inoculation, be transferred to then under the illumination and cultivate a week, explant begins to expand, and grows blade.Starting medium is: MS+6-BA1.0mg/L+NAA1.0mg/L.
(2) inducing clumping bud
Explant is cut into long 0.5-1.0cm and contains the stem section of an axillalry bud, be inoculated in to be positioned on the increment medium and be transferred to cultivation 40-50d illumination under after cultivating 10d in the dark.
Minimal medium is: MS+KT 1.0mg/L+NAA 0.2mg/L
The increment medium is: MS+KT 1.0mg/L+NAA 0.2mg/L+6-BA2.0mg/L+2,4-D 0.4mg/L
(3) root induction and transplanting
The seedling of long 2.0-2.5cm is inoculated in 1/10MS medium and additional NAA0.5mg/L and IBA0.5mg/L to carry out root induction and cultivates, add 5% banana puree and 0.05% active carbon simultaneously, cultivate and choose the good stalwartness group of root system development after 30 days and cultivate strain and carry out acclimatization and transplants.After the tissue culture bottle that opens wide placed greenhouse lower refining seedling 5～7d, the medium that the flush away root is residual was also heeled in vermiculite, preserves moisture and cultivates 10～15d, and direct transplanting is in the matrix of the precious rock of the equivalent of the poison that disappeared, river sand and peat soil.
The invention provides a kind of new roxburgh anoectochilus terminal bud method for quickly breeding, this method is easy and simple to handle, and raw material are easy to get, and the breeding cycle is short, is applicable to batch production production, has good economic benefit.
Claims (8)
1. medicinal roxburgh anoectochilus terminal bud method for quickly cultivating, its feature comprises following step:
(1) explant is cultivated
A wild roxburgh anoectochilus terminal bud material part is adopted back back artificial planting a period of time and is sterilized from the mountain, a part is without directly sterilization of plantation.Remove plant root and blade earlier, intercept the sections of its terminal bud and lateral bud, wash 20min with sterile distilled water, 75% alcohol rinsing 30s, mercuric chloride vibration sterilization 10～13min of 0.1% uses aseptic water washing 3～6 times again, is cutting on the superclean bench on terminal bud, the lateral bud access startup medium.Place 15d in the dark after the inoculation, be transferred to then under the illumination and cultivate a week, explant begins to expand, and grows blade.
(2) inducing clumping bud
Explant is cut into long 0.5-1.0cm and contains the stem section of an axillalry bud, be inoculated in to be positioned on the increment medium and be transferred to cultivation 40-50d illumination under after cultivating 10d in the dark.
(3) root induction and transplanting
The seedling of long 2.0-2.5cm is inoculated in 1/10MS medium and additional NAA 0.5mg/L and IBA 0.5mg/L to carry out root induction and cultivates, add 5% banana puree and 0.05% active carbon simultaneously, cultivate and choose the good stalwartness group of root system development after 30 days and cultivate strain and carry out acclimatization and transplants.After the tissue culture bottle that opens wide placed greenhouse lower refining seedling 5～7d, the medium that the flush away root is residual was also heeled in vermiculite, preserves moisture and cultivates 10～15d, and direct transplanting is in the matrix of the precious rock of the equivalent of the poison that disappeared, river sand and peat soil.
2. tell as claim (1), the bacterial classification that it is characterized in that setting out is wild roxburgh anoectochilus terminal bud.
3. tell as claim (1), it is characterized in that starting medium and consist of: MS+6-BA 1.0mg/L+NAA1.0mg/L.
4. tell as claim (2), it is characterized in that minimal medium selection MS medium, the increment medium is: MS+KT 1.0mg/L+NAA 0.2mg/L+6-BA 2.0mg/L+2,4-D 0.4mg/L, add 6-BA 2.0mg/L+2 simultaneously, 4-D 0.4mg/L can make the increment multiple reach 8.1.
5. tell as claim (2), it is characterized in that at first being put in the dark and to be transferred to that cultivation can produce a fairly large number of bud of growing thickly under the illumination after cultivating.
6. tell as claim (2), it is characterized in that light application time is 10h/d, intensity of illumination is 2000-3000Lx.
7. tell as claim (3), it is characterized in that selecting the 1/10MS medium, the minimal medium that contains the low concentration mineral salt helps the root induction of tissue cultivating seedling and grows.
8. tell as claim (3), it is characterized in that having added in the root media 4 kinds of inducers, be respectively NAA 0.5mg/L, IBA 0.5mg/L, 5% banana puree and 0.05% active carbon.Can significantly improve the strong sprout of roxburgh anoectochilus terminal bud and take root by adding 4 kinds of inducers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101546375A CN102067817A (en) | 2009-11-23 | 2009-11-23 | Method for quickly cultivating medicinal anectochilus roxburhii |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101546375A CN102067817A (en) | 2009-11-23 | 2009-11-23 | Method for quickly cultivating medicinal anectochilus roxburhii |
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| CN102067817A true CN102067817A (en) | 2011-05-25 |
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| CN2009101546375A Pending CN102067817A (en) | 2009-11-23 | 2009-11-23 | Method for quickly cultivating medicinal anectochilus roxburhii |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907327A (en) * | 2012-11-07 | 2013-02-06 | 厦门加晟生物科技有限公司 | Tissue culture propagation method for anoectochilus roxburghii |
| CN103880532A (en) * | 2014-03-21 | 2014-06-25 | 厦门涌泉科技有限公司 | Hydroponic growing method of anoectochilus roburghii |
| CN104737758A (en) * | 2015-03-30 | 2015-07-01 | 中国科学院华南植物园 | Culture method of companumoea root seeds |
| CN108184675A (en) * | 2018-03-15 | 2018-06-22 | 江苏恒诺园林建设有限公司 | Rapid germination culture medium for primary culture of sequoia zhongshanensis |
| CN109042328A (en) * | 2018-09-07 | 2018-12-21 | 天津大学 | A kind of method of roxburgh anoectochilus terminal bud tissue-culturing quick-propagation |
| CN112470904A (en) * | 2020-12-11 | 2021-03-12 | 广西壮族自治区中国科学院广西植物研究所 | Method for improving germination rate of seeds and survival rate of seedlings of pistacia chinensis bunge |
-
2009
- 2009-11-23 CN CN2009101546375A patent/CN102067817A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907327A (en) * | 2012-11-07 | 2013-02-06 | 厦门加晟生物科技有限公司 | Tissue culture propagation method for anoectochilus roxburghii |
| CN102907327B (en) * | 2012-11-07 | 2013-09-04 | 厦门加晟生物科技有限公司 | Tissue culture propagation method for anoectochilus roxburghii |
| CN103880532A (en) * | 2014-03-21 | 2014-06-25 | 厦门涌泉科技有限公司 | Hydroponic growing method of anoectochilus roburghii |
| CN103880532B (en) * | 2014-03-21 | 2015-10-28 | 厦门涌泉科技有限公司 | A kind of water planting implantation methods of Anoectochilus roxburghii |
| CN104737758A (en) * | 2015-03-30 | 2015-07-01 | 中国科学院华南植物园 | Culture method of companumoea root seeds |
| CN108184675A (en) * | 2018-03-15 | 2018-06-22 | 江苏恒诺园林建设有限公司 | Rapid germination culture medium for primary culture of sequoia zhongshanensis |
| CN108184675B (en) * | 2018-03-15 | 2021-09-07 | 江苏恒诺园林建设有限公司 | Rapid germination culture medium for primary culture of sequoia zhongshanensis |
| CN109042328A (en) * | 2018-09-07 | 2018-12-21 | 天津大学 | A kind of method of roxburgh anoectochilus terminal bud tissue-culturing quick-propagation |
| CN112470904A (en) * | 2020-12-11 | 2021-03-12 | 广西壮族自治区中国科学院广西植物研究所 | Method for improving germination rate of seeds and survival rate of seedlings of pistacia chinensis bunge |
| CN112470904B (en) * | 2020-12-11 | 2023-03-24 | 广西壮族自治区中国科学院广西植物研究所 | Method for improving germination rate of seeds and survival rate of seedlings of pistacia chinensis bunge |
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Application publication date: 20110525 |