CN105309311A - Method for breeding improved variety of scrophularia ningpoensis Hemsl. - Google Patents
Method for breeding improved variety of scrophularia ningpoensis Hemsl. Download PDFInfo
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- CN105309311A CN105309311A CN201510747917.2A CN201510747917A CN105309311A CN 105309311 A CN105309311 A CN 105309311A CN 201510747917 A CN201510747917 A CN 201510747917A CN 105309311 A CN105309311 A CN 105309311A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention discloses a method for breeding an improved variety of scrophularia ningpoensis Hemsl., and solves problems that due to long time asexual reproduction of scrophularia ningpoensis Hemsl. in the prior art, virus accumulation is serious, and the yield and the quality are reduced. The method comprises the following steps: 1), screening plants, wherein the pest-free plants are selected; 2) pre-treatment and disinfection, wherein scale-peeled buds are soaked with the 20-time sodium hypochlorite solution for 8-10 min; 3) stem tip peeling, wherein each stem tip with 1-2 leaf primordium are cut; 4) cultivation, wherein the stem tips are placed in a culture medium for cultivation; 5) exercised seedling transplantation, wherein the seedlings are cultivated in the sun, then the seedlings are transplanted to a matrix to be cultivated for 15 days, and finally the seedlings are transplanted to a field. Compared with the conventional asexual reproduction method for scrophularia ningpoensis Hemsl., the yield and the quality of the scrophularia ningpoensis Hemsl. seedlings cultivated by the method are greatly improved.
Description
Technical field
The invention belongs to Chinese herbal medicine field of planting, be specifically related to a kind of radix scrophulariae stock breeding method.
Background technology
Radix scrophulariae is the dry root of goatweed radix scrophulariae (ScrophularianingpoensisHemsl.), begin to be loaded in Shennong's Herbal, there is cool blood enriching yin, effect of purging intense heat and detonicating, cough for consumption of YIN caused by febrile disease, febrile virulent maculae, hectic fever due to yin labor clinically, the disease such as hot eyes, pharyngalgia, diphtheria, carbuncle sore tumefacting virus, be China endemic species, at present only in Chinese natural distribution, whole nation most area all has cultivation, main product in Zhejiang, Anhui, Hubei, the ground such as Guizhou.For conventional bulk medicinal materials, as the raw material of Chinese patent drug, tens kinds of Chinese patent drugs such as tianwangbuxin pills, Tianma Wan, Mailuoning, clearing throat mixture can be produced, now also apply to Cosmetic Manufacture, as plant lubricant, antifreeze frost, crease-proof cream etc.
The modes of reproduction of wild radix scrophulariae utilizes seed to carry out sexual propagation, and medicinal herb grower is in order to preserve fine quality, improve output and shorten growth cycle etc., cultivation radix scrophulariae all adopts vegetative mode, but lack the purification and rejuvenation of kind for many years, virus accumulation is serious, makes the damage by disease and insect incidence of radix scrophulariae increase gradually, therefore need radix scrophulariae to carry out harmless treatment, obtain virus-free seedling and breed.
Plant cell has totipotency, and namely each plant cell is containing whole gene that can produce whole plant.Theoretically, as long as condition is suitable, can Differentiation containing whole genetic cell, produce whole plant.Be generally nontoxic in the meristematic tissue of stem apex and the tip of a root or only containing the virion of extremely low concentration, and in older tissue, viral level improve with the increase from stem apex distance.Principle adopts stem-tip tissue to cultivate and can obtain nontoxic plant accordingly.Utilize this principle, radix scrophulariae can be carried out stock breeding, cultivate virus-free seedling.
Summary of the invention
The invention is intended to provide a kind of radix scrophulariae stock breeding method, because of long-term vegetative propagation, make the problem that virus accumulation is serious to solve radix scrophulariae in prior art.
A kind of radix scrophulariae stock breeding method in this programme, is characterized in that, comprise the following steps:
1) plant screening: when radix scrophulariae is ripe, select the plant without damage by disease and insect;
2) pretreatment and sterilization: adularescent scale parcel got by the radix scrophulariae screened, without cracking, and length is greater than the sub-bud of 3cm, clean by clean water, successively peel off scale, the sub-bud 30s of alcohol rinsing with 70%, then puts into 20 times of liquor natrii hypochloritises and soaks 8-10min, and with aseptic water washing 3-5 time;
3) stem apex is peeled off: by step 2) under the stem apex of sub-bud is placed in microscope after process, with the tweezers of calcination cooling again after alcohol-pickled and scissors, it is processed, elevator bud outer foil, cut the stem apex of 0.1 ~ 0.3mm with 1 ~ 2 leaf primordium, then clean its 3-4 time with sterile distilled water;
4) cultivate: the stem apex obtained in step 3) is put into inducing culture and cultivates, described inducing culture is MS+BA1.5mg/L+NAA1.0mg/L, by the time after growing indefinite bud, continue to cultivate 35 ~ 40 days in inducing culture, to growing Multiple Buds, until Multiple Buds is cut into 2 leaves when growing into 3 ~ 4cm, be about the segment access proliferated culture medium of 1.5cm, proliferated culture medium is MS full dose medium, it within 20 days, is a switching cycle, after propagation to suitable quantity, 30 strains are divided by seedling to be a collection of, criticize in access root media, described root media is 1/2MS+IBA0.05mg/L, be cultured to adularescent root system to grow,
5) hardening is transplanted: the seedling taken root is put into sunlight lower refining seedling 3 days, take out seedling, wash the medium residue on root with water, be then transplanted to earth: peat: perlitic weight ratio is in the matrix of 1:1:1,, transplant to land for growing field crops after 15 days until growth of seedling.
Beneficial effect of the present invention:
The present invention is the mode by Shoot Tip Culture, obtain excellent virus-free radix scrophulariae seedling, plant, plant strain growth is healthy and strong, growing way is consistent, leaf dark green, root system expands, and the incidence of damage by disease and insect only has about 8%, well below the damage by disease and insect incidence of common vegetative propagation about 35%, and output comparatively general planting can increase by 30%, the quality of radix scrophulariae has great lifting, by continuous switching, can obtain a large amount of high-quality seedling, therefore can realize planting needs on a large scale, substantially increase the seed output and quality of radix scrophulariae.
Further, the condition of culture of described breeding phase is temperature 24 ~ 28 DEG C, intensity of illumination 1500 ~ 1800LUX, when stable lower than 24 DEG C, intensity of illumination lower than 1500LUX time, cultivate poor growth, and when temperature be greater than 28 DEG C, intensity of illumination be greater than 1800LUX time, there will be dead phenomenon in cultivating process, therefore select temperature 24 ~ 28 DEG C, intensity of illumination 1500 ~ 1800LUX is optimum condition.
Further, add the root-growing agent that concentration is 0.1 ~ 0.3mg/L in described root media, add root-growing agent, the quantity that can increase its rooting rate He take root, strengthen the success rate and seedling quality of cultivating.
Embodiment
Below by embodiment, the present invention is further detailed explanation:
Control group
In the radix scrophulariae of results the previous year, choose the sub-bud that white does not ftracture, is greater than 3cm without damage by disease and insect, length, planted by traditional implantation methods.
Embodiment 1
A kind of radix scrophulariae stock breeding method in this programme, is characterized in that, comprise the following steps:
1) plant screening: when radix scrophulariae is ripe, select the plant without damage by disease and insect;
2) pretreatment and sterilization: adularescent scale parcel got by the radix scrophulariae screened, without cracking, and length is greater than the sub-bud of 3cm, clean by clean water, successively peel off scale, the sub-bud 30s of alcohol rinsing with 70%, then puts into 20 times of liquor natrii hypochloritises and soaks 9min, and with aseptic water washing 4 times;
3) stem apex is peeled off: by step 2) under the stem apex of sub-bud is placed in microscope after process, with the tweezers of calcination cooling again after alcohol-pickled and scissors, it is processed, elevator bud outer foil, cuts the stem apex of 0.2mm with 2 leaf primordium, then cleans its 3 times with sterile distilled water;
4) cultivate: the stem apex obtained in step 3) is put into inducing culture and cultivates, described inducing culture is MS+BA1.5mg/L+NAA1.0mg/L, by the time after growing indefinite bud, continue to cultivate 38 days in inducing culture, to growing Multiple Buds, until Multiple Buds is cut into 2 leaves when growing into 3cm, be about the segment access proliferated culture medium of 1.5cm, proliferated culture medium is MS full dose medium, it within 20 days, is a switching cycle, after propagation to suitable quantity, 30 strains are divided by seedling to be a collection of, access in root media in batches, described root media is 1/2MS+IBA0.05mg/L, and in root media, add the root-growing agent that concentration is 0.2mg/L, be cultured to white root system to grow, condition of culture is temperature 26 DEG C, intensity of illumination 1600LUX
5) hardening is transplanted: the seedling taken root is put into sunlight lower refining seedling 3 days, then seedling is taken out, wash the medium residue on root with water, be then transplanted to earth: peat: perlite is in the matrix of 1:1:1, the seedling after surviving transplants land for growing field crops after growth is stable.
Get two mu of soils, plant respectively with the seedling that sub-bud and the embodiment 1 of control group are cultivated, statistics damage by disease and insect incidence and output, data are as shown in table 1:
As table 1 data, adopt and originally execute the seedling that example cultivates and plant, the damage by disease and insect incidence of radix scrophulariae can reduce 26.5%, per mu yield can improve 30.8%, as can be seen here, the method adopting stem apex to cultivate, carries out out innoxious to radix scrophulariae, significantly can reduce the damage by disease and insect incidence of radix scrophulariae, and improve output.
Above-described is only embodiments of the invention, and in scheme, the general knowledge such as known concrete structure and characteristic does not do too much description at this.Should be understood that; for a person skilled in the art, under the prerequisite not departing from structure of the present invention, some distortion and improvement can also be made; these also should be considered as protection scope of the present invention, and these all can not affect effect of the invention process and practical applicability.The protection domain that this application claims should be as the criterion with the content of its claim, and the embodiment in specification etc. record the content that may be used for explaining claim.
Claims (3)
1. a radix scrophulariae stock breeding method, is characterized in that, comprises the following steps:
1) plant screening: when radix scrophulariae is ripe, select the plant without damage by disease and insect;
2) pretreatment and sterilization: adularescent scale parcel got by the radix scrophulariae screened, without cracking, and length is greater than the sub-bud of 3cm, clean by clean water, successively peel off scale, the sub-bud 30s of alcohol rinsing with 70%, then puts into 20 times of liquor natrii hypochloritises and soaks 8-10min, and with aseptic water washing 3-5 time;
3) stem apex is peeled off: by step 2) under the stem apex of sub-bud is placed in microscope after process, after alcohol-pickled, then with calcination cooling tweezers and scissors it is processed, elevator bud outer foil, cut the stem apex with 1 ~ 2 leaf primordium, then clean its 3-4 time with sterile distilled water;
4) cultivate: the stem apex obtained in step 3) is put into inducing culture and cultivates, described inducing culture is MS+BA1.5mg/L+NAA1.0mg/L, by the time after growing indefinite bud, continue to cultivate 35 ~ 40 days in inducing culture, to growing Multiple Buds, until Multiple Buds is cut into 2 leaves when growing into 3 ~ 4cm, be about the segment access proliferated culture medium of 1.5cm, proliferated culture medium is MS full dose medium, it within 20 days, is a switching cycle, after propagation to suitable quantity, 30 strains are divided by seedling to be a collection of, access in root media in batches, described root media is 1/2MS+IBA0.05mg/L, be cultured to adularescent root system to grow,
5) hardening is transplanted: the seedling taken root is put into sunlight lower refining seedling 3 days, take out seedling, wash the medium residue on root with water, be then transplanted to earth: peat: perlitic weight ratio is in the matrix of 1:1:1,, transplant to land for growing field crops after 15 days until growth of seedling.
2. radix scrophulariae stock breeding method according to claim 1, is characterized in that, the condition of culture of described breeding phase is temperature 24 ~ 28 DEG C, intensity of illumination 1500 ~ 1800LUX.
3. radix scrophulariae stock breeding method according to claim 1, is characterized in that, adds the root-growing agent that concentration is 0.1 ~ 0.3mg/L in described root media.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830926A (en) * | 2016-04-13 | 2016-08-10 | 广东金作农业科技有限公司 | Tissue culture and rapid propagation method of striga asiatica |
| CN105850739A (en) * | 2016-04-13 | 2016-08-17 | 广东金作农业科技有限公司 | Asiatic witchweed cultivation method |
| CN106069763A (en) * | 2016-06-25 | 2016-11-09 | 杨文平 | The pierre method of Radix Scrophulariae |
| CN106106150A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | Cultivated fine seed strains by the tip of a root method of Radix Scrophulariae |
| CN106106151A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | The prevalent variety cultivation method of Radix Sophorae Flavescentis |
| CN109089808A (en) * | 2018-09-21 | 2018-12-28 | 玉屏县康耀药业有限公司 | A kind of cultural method of radix scrophulariae |
| CN109156346A (en) * | 2018-08-31 | 2019-01-08 | 江苏农林职业技术学院 | Hundred can spend axillary bud micro-propagation method |
| CN116584373A (en) * | 2023-06-19 | 2023-08-15 | 镇坪县农业科学研究所 | Radix scrophulariae hybridization method |
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| CN104365483A (en) * | 2014-11-20 | 2015-02-25 | 安徽省农业科学院园艺研究所 | Scrophularia ningpoensis hemsl breeding method |
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- 2015-11-06 CN CN201510747917.2A patent/CN105309311A/en active Pending
Patent Citations (1)
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| CN104365483A (en) * | 2014-11-20 | 2015-02-25 | 安徽省农业科学院园艺研究所 | Scrophularia ningpoensis hemsl breeding method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830926A (en) * | 2016-04-13 | 2016-08-10 | 广东金作农业科技有限公司 | Tissue culture and rapid propagation method of striga asiatica |
| CN105850739A (en) * | 2016-04-13 | 2016-08-17 | 广东金作农业科技有限公司 | Asiatic witchweed cultivation method |
| CN105830926B (en) * | 2016-04-13 | 2017-10-24 | 广东金作农业科技有限公司 | A kind of quick breeding method for tissue culture of witchweed |
| CN106069763A (en) * | 2016-06-25 | 2016-11-09 | 杨文平 | The pierre method of Radix Scrophulariae |
| CN106106150A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | Cultivated fine seed strains by the tip of a root method of Radix Scrophulariae |
| CN106106151A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | The prevalent variety cultivation method of Radix Sophorae Flavescentis |
| CN106069763B (en) * | 2016-06-25 | 2018-04-24 | 杨文平 | The pierre method of radix scrophulariae |
| CN109156346A (en) * | 2018-08-31 | 2019-01-08 | 江苏农林职业技术学院 | Hundred can spend axillary bud micro-propagation method |
| CN109156346B (en) * | 2018-08-31 | 2021-09-07 | 江苏农林职业技术学院 | Method for micropropagation of axillary buds of Cockflower |
| CN109089808A (en) * | 2018-09-21 | 2018-12-28 | 玉屏县康耀药业有限公司 | A kind of cultural method of radix scrophulariae |
| CN116584373A (en) * | 2023-06-19 | 2023-08-15 | 镇坪县农业科学研究所 | Radix scrophulariae hybridization method |
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Application publication date: 20160210 |