CN105830884B - A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud - Google Patents

A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud Download PDF

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Publication number
CN105830884B
CN105830884B CN201610186069.7A CN201610186069A CN105830884B CN 105830884 B CN105830884 B CN 105830884B CN 201610186069 A CN201610186069 A CN 201610186069A CN 105830884 B CN105830884 B CN 105830884B
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water
terminal bud
roxburgh anoectochilus
anoectochilus terminal
ecology
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CN105830884A (en
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叶乃兴
郑知临
林庆良
郑德勇
林心涛
屈艳勤
杨江帆
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Fujian Agriculture and Forestry University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to a kind of methods of Ecology water-surface cultivation roxburgh anoectochilus terminal bud, including selection Ecology environment, Ecology facility cultivation, the preparation of roxburgh anoectochilus terminal bud foliar fertilizer and fertilizing method.The present invention is suitble to the ecological environment of roxburgh anoectochilus terminal bud growth by selection, adds some simple cultivating facilities, achievees the purpose that Emulational culture roxburgh anoectochilus terminal bud, reaches the maximization of benefit by preparing roxburgh anoectochilus terminal bud special foliar fertilizer and exploring fertilizing method to reduce cost.The method of the Ecology water-surface cultivation roxburgh anoectochilus terminal bud of the present invention, keeps the toxigenic capacity of roxburgh anoectochilus terminal bud lower, nursery stock is more strong, and quality is more preferable.Since the weight of plant and its planting basin body all itself is undertaken by floating body, without other affiliated facilities, it is at low cost, easy for installation and convenient for management.Very crucial practical technique is provided for roxburgh anoectochilus terminal bud production.

Description

A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud
Technical field
The present invention relates to a kind of arable farming methods, and in particular to a kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud belongs to Technical field of crop cultivation.
Background technology
Roxburgh anoectochilus terminal bud Anoectochilus roxburghii also known as Shorthairy Antenoron, rough melic herb are that orchid family Anoectochilus Blume is perennial Rare traditional Chinese medicine, main product have clearing heat and cooling blood, removing dampness and detoxicating and other effects in Fujian, Zhejiang, Taiwan, Jiangxi, Guizhou and other places, use In treatment diabetes, ephritis, acute, chronic hepatitis etc., in the civil laudatory title for enjoying " king of medicine ".Modern research shows that containing in roxburgh anoectochilus terminal bud There is anoectochilus roxburghii glycosides isoreactivity ingredient, has and repair damaged islet cells, restore the pharmacological activity of normal insulin secretion, with gold thread Lotus is that the Chinese patent drug of primary raw material clinically has been used for the treatment of diabetes such as compound roxburgh anoectochilus terminal bud capsule.Due to roxburgh anoectochilus terminal bud kind Son is small, and embryonic development is incomplete, and breeding potential is extremely low under natural conditions, and through developing for many years, wild resource has been on the verge of exhaustion at present. Just become effective way of its wild resource of protection and rational exploitation and utilization with tissue-culturing rapid propagation and transplantation technique large scale cultivating roxburgh anoectochilus terminal bud Diameter.
The quick breeding technology of artificial organ culture at present has been gradually improved, and can have been cultivated under the artificial condition of laboratory big The roxburgh anoectochilus terminal bud test tube seedling of amount, therefore under the premise of solving source of seedling, practicing transplantation of seedlings technology just becomes a period of time from now on The emphasis of interior research, and that there is also test tube seedling transplanting survival rates in mass producing at present is not high, upgrowth situation is undesirable, at The problems such as this is higher.
Invention content
The object of the present invention is to provide a kind of methods of Ecology water-surface cultivation roxburgh anoectochilus terminal bud.
The purpose of the present invention is what is be realized by the following method.
The method of a kind of Ecology water-surface cultivation roxburgh anoectochilus terminal bud of the present invention, it is characterised in that steps are as follows:
1. selecting Ecology environment:Select height above sea level be not less than 400 meters, January temperature on average >=10 DEG C, July average air Temperature≤27 DEG C, air humidity >=70%, the water surface planting under the short small stream side tree shade of illumination straight fluidic time, or the drop using topography The vacant lot for introducing the water into periphery carries out facility cultivation;
2. Ecology facility cultivation:
It selects the water surface under the tree shade of small stream side for Ecology environment, carries out facility cultivation;Or it is put up on the vacant lot on small stream side Vinyl house, on big ceiling plus shading screen;Build pond in canopy, the stream of upstream enters pond water storage from water inlet, extra water from Water outlet flows into the stream in downstream;Using the Ecology environment being manually arranged, facility cultivation is carried out;The facility cultivation: Furrow are connected by rope with floating body on the water surface, the both ends of furrow are articulated on fixed mark post, floating body with the variation of water level and It floats up and down;There is multiple plantation apertures, hole diameter 1cm on floating body;When planting roxburgh anoectochilus terminal bud, by the root water of its bottle seedling Straw bag is included in after living in aperture, and planting density is 4cm × 4cm;
3. roxburgh anoectochilus terminal bud foliar fertilizer is prepared:
A after claiming the soya bean of 100-110g to be soaked in water overnight, takes out soya bean and adds the water of 2-3ml, with pulverizer by its dozen It at paste, is packed into container and adds water to 900ml, seal, stand no less than 1 month, then with two layers of filtered through gauze, filtrate is taken to use It is spare that water is settled to 1000ml;
B claims potassium nitrate 440-460mg, ammonium nitrate 340-360mg, potassium dihydrogen phosphate 49-51mg, magnesium sulfate 79-81mg, Boric acid 14-16mg, manganese sulfate 28-32mg, zinc sulfate 14-16mg, sodium molybdate 2.3-2.6mg, copper sulphate 0.23-0.26mg, second Edetate disodium 37.2-37.4mg ferrous sulfate 27.6-27.9mg inositol 210-230mg, glycine 2-2.2mg, hydrochloric acid It is mixed after thiamine 0.1-0.12mg, puridoxine hydrochloride 0.4-0.6mg water dissolution and is settled to 10L;
C. the use of preceding is 1 by weight by A, B solution:10 ratio mixing, as roxburgh anoectochilus terminal bud foliar fertilizer;
4. fertilizing method:
Roxburgh anoectochilus terminal bud bottle seedling starts fertilization of leaves after planting 15 days, will be sprayed after the water of the 4 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer On blade face, sprayed again every 10-15 days primary;
After roxburgh anoectochilus terminal bud bottle seedling is planted 45 days, it will be sprayed on blade face after the water of the 2 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer, it Afterwards, primary every sprinkling in 10-15 days, until harvest.
The application effect of the present invention:
1. influence of the different cultivation conditions to roxburgh anoectochilus terminal bud bottle seedling transplanting survival rate
Roxburgh anoectochilus terminal bud is middle heliophobous plant, happiness dim light, high humidity, nice and cool transplanting environment.The growth preference temperature of roxburgh anoectochilus terminal bud exists 20~24 DEG C, high temperature easily leads to death.It is measured using air-flow method, roxburgh anoectochilus terminal bud light saturation point is 5000Lx, and light compensation point is 400Lx, maximum light requirement are no more than 6000Lx.According to these conditions, select in Fuzhou City, Fujian Province Min Hou Xian Qishan tourist districts, 400 meters of height above sea level, forest carry out lid rate height, and summer temperature is nice and cool, and locality has wild gold lotus, ecological environment to be well suited for the life of roxburgh anoectochilus terminal bud It is long.Water surface planting under the short small stream side tree shade of an illumination straight fluidic time.
It is floating body with foamed plastics, furrow is connected into rope between floating body, a both ends installation iron ring for furrow is sleeved on fixed On mark post, floating body can change with the variation of water level.Foamed plastics is equipped with multiple implantation holes, aperture 1cm.Roxburgh anoectochilus terminal bud It is included in aperture after being encased with water plant the root of bottle seedling.Planting density is 4cm × 4cm.
Influence for more different cultivation conditions to roxburgh anoectochilus terminal bud bottle seedling transplanting survival rate and increment.We devise three The different cultivation condition of kind, comparison result are as shown in table 1.Wherein, the matrix of substrate culture is peat soil:Husky ﹦ 1:1 (weight Than);Water circulating cultivation is to carry out water cycle using water pump in greenhouse.Statistics survival rate and yield after plantation 6 months.
Test result shows that the survival rate of Ecology water-surface cultivation of the invention and water circulating cultivation obviously compares substrate culture Height, so yield is also naturally high much.
Influence of the different cultivation conditions of table 1 to roxburgh anoectochilus terminal bud bottle seedling transplanting survival rate
Influence of the various concentration and Different Fertilization mode of 2. foliar fertilizer of table to roxburgh anoectochilus terminal bud different stages of growth
2. the influence of the various concentration and Different Fertilization mode of foliar fertilizer to roxburgh anoectochilus terminal bud different stages of growth.
In order to find suitable foliar fertilizer concentration, fertilizing method and interval time, three factors, three are with (3) 4 orthogonal arrages of L9 Horizontal orthogonal test.Each processing combination counts total weight after planting 200 plants, 6 months.Test result is as shown in table 2, foliar fertilizer Concentration survival rate is influenced maximum, concentration more high-survival rate is lower.Since nursery stock death is all in early stage, so, it is applied in early days It is preferable with the foliar fertilizer of low concentration.In terms of the interval time of fertilising, preferably 10 days and 15 days, survival rate is all 94% or more. From the point of view of ultimate output, preferably 1/2 concentration, average product is at 260.7 grams.Fertilising interval time is also with 10 days and 15 days Preferably considers each factor, and bottle seedling uses 1/4 foliar fertilizer after planting 15 days, once every sprinkling in 15 days, continuous 2 times.Then It is primary every sprinkling in 15 days with 1/2 nutrient solution, until harvest.1/2,1/4 concentration described in table 2 refers to 2 times, 4 times of weight Water dilute foliar fertilizer.
3. influence of the different foliar fertilizers of application to roxburgh anoectochilus terminal bud survival rate and yield
Select the formula of MS culture mediums and classical improvement Huo Gelande formulas and inventive formulation do it is a compared with, bottle seedling kind It is primary every fertilising in 15 days after planting 15 days, continuous 10 times.Test result is as shown in table 3, and concentration has apparent negative with survival rate It closes.It is best from the point of view of foliar fertilizer type, or with inventive formulation, either survival rate or yield is all apparently higher than it Its two kinds of formula.
Influence of the different foliar fertilizers of table 3 to roxburgh anoectochilus terminal bud survival rate and yield
* MS is 1962, MS culture mediums, not sugaring and agar powder disclosed in Murashige and Skoog.
* improves Huo Gelan formulas:Four water-calcium nitrate 945mg/L, potassium nitrate 506mg/L, ammonium nitrate 80mg/L, phosphoric acid Potassium dihydrogen 136mg/L, epsom salt 493mg/L, iron salt solutions 2.5ml, liquid microelement 5ml, pH=6.0.
Iron salt solutions:Ferrous sulfate heptahydrate 2.78g, disodium ethylene diamine tetraacetate 3.73g, distilled water 500ml, pH=5.5.
Liquid microelement:Potassium iodide 0.83mg/l, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, molybdenum Sour sodium 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L.
The present invention has the advantages that:
1. a kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud using the present invention, can be with simplified cultivation link, with water planting It compares, the water pump and power consumption of water cycle is saved, to reduce cultivation cost.
2. of the invention be mainly absorbed and utilized nitrogen and phosphorus element progress metabolic activity using roxburgh anoectochilus terminal bud root system, water body can be removed Middle nitrogen, phosphorus nutrition substance, have the function that purifying water body.
3. easy to operate, highly practical, small investment of the invention is suitble to promote in the area for having wild gold lotus.
The present invention is suitble to the ecological environment of roxburgh anoectochilus terminal bud growth by selection, adds some simple cultivating facilities, reaches The purpose of Emulational culture roxburgh anoectochilus terminal bud is reached by preparing roxburgh anoectochilus terminal bud special foliar fertilizer and exploring fertilizing method with reducing cost The maximization of benefit.The method of the Ecology water-surface cultivation roxburgh anoectochilus terminal bud of the present invention, keeps the toxigenic capacity of roxburgh anoectochilus terminal bud lower, nursery stock is more Strong, quality is more preferable.Since the weight of plant and its planting basin body all itself is undertaken by floating body, without other affiliated facilities, at This is low, easy for installation and convenient for management.Very crucial practical technique is provided for roxburgh anoectochilus terminal bud production.
Specific implementation mode
In order to which the present invention is furture elucidated rather than the limitation present invention, it is illustrated below in conjunction with example.
Embodiment one:A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud, includes the following steps:
1, Ecology environment is selected:
Select height above sea level be not less than 400 meters, January temperature on average >=10 DEG C, July temperature on average≤27 DEG C, air humidity >=70%, the water surface planting under the short small stream side tree shade of illumination straight fluidic time;
2. Ecology facility cultivation:
It selects the water surface under the tree shade of small stream side for Ecology environment, carries out facility cultivation;
3. roxburgh anoectochilus terminal bud foliar fertilizer is prepared:
A after claiming the soya bean of 100g to be soaked in water overnight, takes out 2g soya beans and the water of 2g is added to be broken into paste with pulverizer Shape is packed into container and adds water to 900ml, seals, and stands no less than 1 month, then with two layers of filtered through gauze, takes filtrate with originally It is spare that water is settled to 1000ml;
B claims potassium nitrate 450mg, ammonium nitrate 350mg, potassium dihydrogen phosphate 50mg, magnesium sulfate 80mg, boric acid 15mg, manganese sulfate 30mg, zinc sulfate 15mg, sodium molybdate 2.5mg, copper sulphate 0.25mg, disodium ethylene diamine tetraacetate 37.3mg ferrous sulfate 27.8mg It is mixed after inositol 200mg, glycine 2mg, thiamine hydrochloride 0.1mg, puridoxine hydrochloride 0.5mg water dissolution and is settled to 10L;
C. the use of preceding is 1 by weight by A, B solution:10 ratio mixing, as roxburgh anoectochilus terminal bud foliar fertilizer;
4. fertilizing method:
Roxburgh anoectochilus terminal bud bottle seedling starts fertilization of leaves after planting 15 days, will be sprayed after the water of the 4 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer On blade face, sprayed again every 10 days primary;
After plantation 45 days, it will be sprayed on blade face after the water of the 2 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer, later, every 10 Its sprinkling is primary, until harvest.
By the method for the present embodiment, after 1000 plants, 6 months of transplanting roxburgh anoectochilus terminal bud bottle seedling, it is public that roxburgh anoectochilus terminal bud fresh weight 1.428 is harvested altogether Jin.
Embodiment two:A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud, includes the following steps:
1, Ecology environment is selected:Select height above sea level be not less than 400 meters, January temperature on average >=10 DEG C, July average air Temperature≤27 DEG C, air humidity >=70%, the vacant lot that periphery is introduced the water into using the drop of topography carry out facility cultivation;
2. Ecology facility cultivation:
Vinyl house is put up in selection on the vacant lot on small stream side, adds shading screen on big ceiling;Pond, the stream of upstream are built in canopy Enter pond water storage from water inlet, extra water is from the stream that water outlet flows into downstream;Using the Ecology environment being manually arranged, Carry out facility cultivation;
3. roxburgh anoectochilus terminal bud foliar fertilizer is prepared:
A after claiming the soya bean of 110g to be soaked in water overnight, takes out 2g soya beans and the water of 2g is added to be broken into paste with pulverizer Shape is packed into container and adds water to 900ml, seals, and stands no less than 1 month, then with two layers of filtered through gauze, takes filtrate with originally It is spare that water is settled to 1000ml;
B claims potassium nitrate 460mg, ammonium nitrate 360mg, potassium dihydrogen phosphate 51mg, magnesium sulfate 81mg, boric acid 16mg, manganese sulfate 31mg, zinc sulfate 16mg, sodium molybdate 2.6mg, copper sulphate 0.26mg, disodium ethylene diamine tetraacetate 37.4mg ferrous sulfate 27.9mg It mixes and is settled to after inositol 230mg, glycine 2.1mg, thiamine hydrochloride 0.12mg, puridoxine hydrochloride 0.6mg water dissolution 10L;
C. the use of preceding is 1 by weight by A, B solution:10 ratio mixing, as roxburgh anoectochilus terminal bud foliar fertilizer;
4. fertilizing method:
Roxburgh anoectochilus terminal bud bottle seedling starts fertilization of leaves after planting 15 days, will be sprayed after the water of the 4 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer On blade face, sprayed again every 15 days primary;
After plantation 45 days, it will be sprayed on blade face after the water of the 2 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer, later, every 15 Its sprinkling is primary, until harvest.
By the method for the present embodiment, after 1000 plants, 6 months of transplanting roxburgh anoectochilus terminal bud bottle seedling, 1.643 kilograms of fresh weight is harvested altogether.

Claims (2)

1. a kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud, it is characterised in that steps are as follows:
(1) Ecology environment is selected:Select height above sea level be not less than 400 meters, January temperature on average >=10 DEG C, July temperature on average≤ 27 DEG C, air humidity >=70%, the water surface planting under the short small stream side tree shade of illumination straight fluidic time, or using topography drop by water The vacant lot for being introduced into periphery carries out facility cultivation;
(2) Ecology facility cultivation:It selects the water surface under the tree shade of small stream side for Ecology environment, carries out facility cultivation;Or in small stream Vinyl house is put up on the vacant lot on side, adds shading screen on big ceiling;Pond is built in canopy, the stream of upstream enters pond from water inlet and stores Water, extra water is from the stream that water outlet flows into downstream;Using the Ecology environment being manually arranged, facility cultivation is carried out;Institute State facility cultivation:Furrow are connected by rope with floating body on the water surface, the both ends of furrow are articulated on fixed mark post, floating body with The variation of water level and float up and down;There is multiple plantation apertures, hole diameter 1cm on floating body;When planting roxburgh anoectochilus terminal bud, by its bottle The root of seedling is included in after being encased with water plant in aperture, and planting density is 4cm × 4cm;
(3) roxburgh anoectochilus terminal bud foliar fertilizer is prepared:
A after claiming the soya bean of 100-110g to be soaked in water overnight, takes out the water that soya bean adds 2-3ml, paste is broken into pulverizer Shape is packed into container and adds water to 900ml, seals, and stands no less than 1 month, then with two layers of filtered through gauze, takes filtrate water fixed Hold spare to 1000ml;
B claims potassium nitrate 440-460mg, ammonium nitrate 340-360mg, potassium dihydrogen phosphate 49-51mg, magnesium sulfate 79-81mg, boric acid 14-16mg, manganese sulfate 28-32mg, zinc sulfate 14-16mg, sodium molybdate 2.3-2.6mg, copper sulphate 0.23-0.26mg, ethylenediamine Tetraacethyl disodium 37.2-37.4mg, ferrous sulfate 27.6-27.9mg, inositol 210-230mg, glycine 2-2.2mg, hydrochloric acid sulphur It is mixed after amine element 0.1-0.12mg, puridoxine hydrochloride 0.4-0.6mg water dissolution and is settled to 10L;
C. the use of preceding is 1 by weight by A, B solution:10 ratio mixing, as roxburgh anoectochilus terminal bud foliar fertilizer;
(4) fertilizing method:
Roxburgh anoectochilus terminal bud bottle seedling starts to apply foliar fertilizer after planting 15 days, will be sprayed at after the water of the 4 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer On blade face, sprayed again every 10-15 days primary;
After plantation 45 days, it will be sprayed on blade face after the water of the 2 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer, later, every 10-15 days Sprinkling is primary, until harvest.
2. a kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that the fertilising side Method:
A, roxburgh anoectochilus terminal bud bottle seedling starts to apply foliar fertilizer after planting 15 days, will be sprayed after the water of the 4 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer On blade face, sprayed again every 15 days primary;
B, it after planting 45 days, will be sprayed on blade face after the water of the 2 times of weight dilution of roxburgh anoectochilus terminal bud foliar fertilizer, later, every 15 days Sprinkling is primary, until harvest.
CN201610186069.7A 2016-03-29 2016-03-29 A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud Expired - Fee Related CN105830884B (en)

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CN109618898A (en) * 2019-01-29 2019-04-16 福建农林大学 A kind of method of cultivation under woods roxburgh anoectochilus terminal bud

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